Turkish Journal of Pharmaceutical Sciences最新文献

{"title":"Knowledge Assessment During the Medication Process Use by Older Patients on Clinical Routine: A Pilot Study.","authors":"Margarida Espírito-Santo,&nbsp;Tânia Nascimento,&nbsp;Ezequiel Pinto,&nbsp;M Dulce Estêvão","doi":"10.4274/tjps.galenos.2022.85054","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2022.85054","url":null,"abstract":"<p><strong>Objectives: </strong>The consumption of medicines has been increasing over the last decades. The lack of medication knowledge (MK) may affect the process of medication use and, consequently, may lead to negative health outcomes. This study carried out a pilot study using a new tool to assess MK in older patients in a daily clinical practice.</p><p><strong>Materials and methods: </strong>An exploratory cross-sectional study was conducted, including older patients (≥65 years), taking two or more medicines, followed in a regional clinic. Data were collected during a structured interview, which included an algorithm for assessing MK regarding the identification of the medicines and its use and storage conditions. Health literacy and treatment adherence were also assessed.</p><p><strong>Results: </strong>The study enrolled 49 patients, mainly between 65 and 75 years (n: 33; 67.3%) and polymedicated (n: 40; 81.6%), taking a mean of 6.9 ± 2.8 medicines <i>per</i> day. A lack of MK (score <50%) was observed in 15 (30.6%) participant patients. \"Drug strength\" and \"storage conditions\" were the items which presented the lowest score. MK was positively correlated with higher scores for health literacy and treatment adherence. Younger patients (age <65 years old) also had a higher MK score.</p><p><strong>Conclusion: </strong>This study showed that the applied tool could evaluate the MK of the participants and identified specific gaps regarding MK within the process of medicine use. Further studies, with more participants, will allow the confirmation of these findings and will stimulate the development of specific strategies to improve MK, thus contributing to better health outcomes.</p>","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":"20 1","pages":"1-7"},"PeriodicalIF":1.7,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986945/pdf/TJPS-20-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9111959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Folate-Mediated Paclitaxel Nanodelivery Systems: A Comprehensive Review.","authors":"Ashwini K Bawankule,&nbsp;Amol A Tatode,&nbsp;Pranali S Patil,&nbsp;Milind J Umekar","doi":"10.4274/tjps.galenos.2021.26529","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2021.26529","url":null,"abstract":"<p><p>Paclitaxel (PTX) is used as a viable cancer medication in the chemotherapy of breast, ovarian, lung, bladder, neck, head, and esophageal tumors. The focus of this review is to survey various folate-targeting PTX-loaded nanopreparations in both research and clinical applications. There are diverse nanopreparations, including liposomes, micelles, polymeric nanopreparations, lipid nanopreparations, lipoprotein nanocarriers, and other inorganic nanopreparations for folate-associated PTX tumor targeting. Here, the folate targeting PTX-loaded nanopreparations, which have promising results in the constructive treatment of cancer by reducing toxic side-effects and/or improving effectiveness, was mainly reviewed.</p>","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":"20 1","pages":"58-67"},"PeriodicalIF":1.7,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986944/pdf/TJPS-20-58.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10866126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stability Evaluation of the Biosimilar Monoclonal Antibody Using Analytical Techniques.","authors":"Deniz Demirhan","doi":"10.4274/tjps.galenos.2022.47690","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2022.47690","url":null,"abstract":"Objectives\u0000Determination of the drug substance (DS) and drug product (DP) stability is especially important for biosimilar monoclonal antibodies since it can affect the quality, efficacy, and safety of the drugs. The main objective of this study was to determine the stability of the biosimilar candidate (TUR01) using state-of-the-art (current) analytical techniques.\u0000\u0000\u0000Materials and Methods\u0000Analytical techniques used in this study were isoelectric focusing on capillary electrophoresis, capillary electrophoresis-sodium dodecyl sulfate, size exclusion chromatography-ultra-high performance liquid chromatography, binding affinity, and physicochemical and microbiological tests. DS was kept in polyethylene terephthalate copolyester, glycol modified (PETG) bottles at ≤-65.0°C and 5.0 ± 3.0°C for 18 months, where the pre-filled syringe stability study was conducted at 5.0 ± 3.0°C for 24 months and 25.0 ± 2.0°C/60% ± 5 relative humidity (RH) for 6 months. The accelerated condition for DS was accepted as 5.0 ± 3.0°C, while it was 25.0 ± 2.0°C for the DP.\u0000\u0000\u0000Results\u0000The results indicated that TUR01 DS was stable when it was stored under long-term storage conditions at ≤-65°C and at 5 ± 3°C at least 18 months. Also, TUR01 DP was stable at 5 ± 3°C for 24 months and at 25 ± 2°C with 60.5% RH for 2 months without any significant changes.\u0000\u0000\u0000Conclusion\u0000State-of-the-art analytical techniques proved to be invaluable tools for evaluate the stability of the TUR01 DS and drug product.","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":"20 1","pages":"48-57"},"PeriodicalIF":1.7,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986942/pdf/TJPS-20-48.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10866130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resveratrol-Loaded Microsponge Gel for Wound Healing: <i>In Vitro</i> and <i>In Vivo</i> Characterization.","authors":"Vinita Chandrakant Patole,&nbsp;Devyani Awari,&nbsp;Shilpa Chaudhari","doi":"10.4274/tjps.galenos.2022.93275","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2022.93275","url":null,"abstract":"<p><strong>Objectives: </strong>The study was aimed to formulate resveratrol (RSV) loaded microsponges to deliver drug at the wound site and incorporate it in the <i>Moringa oleifera</i> Lam. (Moringaceae) gel base to provide an appropriate moist environment for wound management. RSV, a stilbenoid that activates sirtuins and cell-signaling regulators involved in the process of wound healing.</p><p><strong>Materials and methods: </strong>Microsponges were prepared by oil in oil emulsion solvent diffusion method by optimizing the independent variables; drug: polymer ratio and volume of internal phase solvent and their effects on entrapment efficiency and particle size. Formulation batches were evaluated for drug content, production yield, entrapment efficiency, and <i>in vitro</i> drug release. The microsponges were further incorporated into <i>M. oleifera</i> gum gel, which was then evaluated for spreadability, viscosity, <i>ex vivo</i> diffusion study and <i>in vivo</i> studies using an excision wound model in rats.</p><p><strong>Results: </strong>Scanning electron microscopy revealed spherical and porous nature of the microsponges <i>in vitro</i>-release study of the optimized batch of RSV microsponges showed 80.88% drug release within 8 h. Differential scanning calorimetry results revealed no drug and polymer interaction during the formation of microsponges. An <i>ex vivo</i> diffusion study through goat skin revealed sustained release of RSV through porous microsponges embedded in the gel base at the wound site. An <i>in vivo</i> study performed using an excision wound model showed wound healing and closure within day 8. Histopathology showed increased re-epithelization and reduced ulceration in RSV microsponge gel-treated group compared with sham operated.</p><p><strong>Conclusion: </strong>RSV microsponge gel delivered the drug at the wound site and the gel base provided a moist environment and influenced cell adhesion, thereby promoting faster wound healing.</p>","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":"20 1","pages":"23-34"},"PeriodicalIF":1.7,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986941/pdf/TJPS-20-23.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10859881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genistein Enhances TRAIL-Mediated Apoptosis Through the Inhibition of XIAP and DcR1 in Colon Carcinoma Cells Treated with 5-Fluorouracil","authors":"T. Çal, S. Aydın Dilsiz, H. Canpınar, Ülkü Ündeğer Bucurgat","doi":"10.4274/tjps.galenos.2023.69649","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2023.69649","url":null,"abstract":"Objectives: Colorectal cancer is one of the most common cancers in the world. However, surgical intervention and chemotherapy provide only limited benefits for the recovery and survival of patients. The anti-carcinogenic effect of genistein has attracted attention due to epidemiological studies showing that soybean consumption is associated with a decrease in incidence of cancer. There are limited studies on the effects of genistein in colorectal carcinoma cells. We aimed to investigate the cytotoxic, genotoxic, and apoptotic effects of genistein in SW480 and SW620 colon adenocarcinoma cells treated with 5-fluorouracil, the basis of chemotherapy, and TRAIL ligand, the mediator of apoptosis, both alone and in combination. Materials and Methods: The cytotoxicity and genotoxicity were determined by MTT assay and comet assay, respectively. The apoptotic effects were evaluated by RT-PCR assay, with the additional use of Annexin V FITC, mitochondrial membrane potential, caspase 3, 8 and 9 activity, reactive oxygen species assay kits. Results: According to our findings, genistein, 5-fluorouracil and TRAIL had synergistic apoptotic effects as a result of DR5 up-regulation, ROS production, and DNA damage, which was mediated by increased caspase 3, 8 and 9 activity and decreased mitochondrial membrane potential. Conclusion: The applied combinations of these compounds may contribute to the resistance problem that may occur in the treatment of colorectal cancer, with the decrease in DcR1 and XIAP genes.","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42116508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deiodinase Type III Polymorphism (Rs1190716) Affects The Therapeutic Response to Levothyroxine Short Title: Deiodinase Type III Gene and Hypothyroidism","authors":"A. H. Mohmmed, Suzanne Jubair, B. Khalaf","doi":"10.4274/tjps.galenos.2022.04876","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2022.04876","url":null,"abstract":"Objectives : Levothyroxine (LT4) is the commonly used treatment for hypothyroidism. Deiodinases enzymes control the metabolism and homeostasis of thyroid hormones (THs). Deiodinases type III (DIO3) gene encodes deiodinase type 3 enzyme (D3), the genetic polymorphisms of this gene could affect the levels of THs and then the response to LT4 treatment. This study aims to investigate the single nucleotide polymorphism (SNP), rs1190716; C>T, of DIO3 as a candidate genetic variant that might affect the clinical response to LT4 treatment. Materials and Methods: Two hundred Iraqi hypothyroid female patients who were aged 40 years or older were enrolled in this cross-sectional study. All of them were already on the LT4 treatment for at least 4 months. Thyroid hormones (thyroxin (T4), triiodothyrionine (T3), revers riiodothyrionine (rT3) and diiodothyrionine (T2)) were estimated. Allele specific-polymerase chain reaction technique was performed to detect the rs1190716; C>T SNP. Results: The genotypes distribution of rs1190716; C>T SNP was 10 (4.5%) for the wild type (CC), 50 (22.7%) for the heterozygous mutant type (TC), and 160 (72.7%) for the homozygous mutant type (TT). The patients were divided into three groups according to their genotypes. Significant differences were found in the levels of T4, T3 and T2 among the groups of the patients (P=0.019, P=0.039, P= 0.032, respectively) Conclusion: The rs1190716; C>T SNP could affect the activity of the D3 enzyme and the metabolic homeostasis of the THs, therefore rs1190716; C>T SNP could have an impact in the therapeutic response to LT4 in Iraqi female patients with primary hypothyroidism. Regarding the DIO3 gene, this is a novel finding, hence further studies are needed to conform it.","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43301743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell Therapy and Investigation of Angiogenesis of Fibroblastswith Collagen Hydrogel on the Healing of Diabetic Wounds","authors":"Abbas Zabihi, Sanaz Pashapour, M. Mahmoodi","doi":"10.4274/tjps.galenos.2022.62679","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2022.62679","url":null,"abstract":"INTRODUCTION: A diabetic ulcer is a common disease in diabetic patients. Due to antibiotic resistance, new therapeutic alternatives are being considered in diabetic foot patients to reduce complications and mortality. This study aimed to evaluate the effect of collagen hydrogel on wound healing process in diabetic rats. METHODS: Diabetic wounds were induced with streptozotocin in all 42 male Wistar rats. The rats were divided into four groups: (a) treated with fibroblast cells, (b) collagen hydrogel, (c) collagen cultured with fibroblast cells, and (d) control group. Microscopic and histological (H&E staining and Mason trichrome staining), measurement of wound surface with Image J, skin density and thickness by the ultrasound probe, and skin elasticity with cutometer tool was used to evaluate the wound healing in a days , 14, and 21 after the treatment. RESULTS: The results showed that the treatment of diabetic wounds with fibroblast cells cultured in collagen hydrogel greatly reduces inflammatory responses in the skin tissue and significantly accelerates the healing process. Also, 21 days after the start of treatment, skin elasticity, thickness and density were higher in the collagen + fibroblast group than in the control group.","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48275892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Cross-Sectional Survey of Knowledge, Attitude, and Practices Regarding Influenza Vaccination Among Jordanians Aged 18–64 With Chronic Diseases","authors":"Ola A Bdair, Izzeddin A. Bdair, Esraa Gogazeh, Ola Al-fawares, M. Alwadi, Rawan Badaineh, Fatima Al-tarawneh","doi":"10.4274/tjps.galenos.2022.61798","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2022.61798","url":null,"abstract":"Background : Influenza is a frequent infectious disease that can be prevented and is linked to significant mortality and morbidity. The most economical way to prevent influenza is through vaccination, although this method is not widely used. This study aimed to assess the seasonal influenza vaccination rates and the knowledge and attitudes of Jordanian adults with chronic illnesses toward the influenza vaccine. Methods : A cross-sectional design was employed. A 26-item online survey was utilized to gather data about the patients' knowledge of and attitudes toward the influenza vaccine as well as their status as influenza vaccine recipients. Results : A total of 19% of the 564 study participants had an influenza vaccination. The majority (81%) of individuals reported inconsistent vaccination uptake. The most important factor affect vaccination is the belief the flu is not a threat (39%) and they were not advised by their doctors about the vaccination (32%). Participants with no health insurance and with public insurance had a lower level of vaccination in comparison with private insurance (p = 0.008). Conclusions : The adult population of Jordan with chronic diseases have subpar immunization rates. Also revealed is a blatant misunderstanding about the value of routine influenza vaccination. These findings emphasize how urgently the public needs to be made aware of the effectiveness of the influenza vaccine.","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49502048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Full Validation of a Novel Liquid Chromatography Electrochemical Detection Method for Simultaneous Determination of Nine Catecholamines in Rat Brain","authors":"Saniye Özcan, Murat Kozanlı, Aysun Geven, Nafiz Öncü Can","doi":"10.4274/tjps.galenos.2022.06606","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2022.06606","url":null,"abstract":"INTRODUCTION: Chemical neurotransmission, managed by neurotransmitters, has a crucial role in brain processes such as fear, memory, learning, and pain, or neuropathologies such as schizophrenia, epilepsy, anxiety/depression, and Parkinson’s disease. The measurement of these compounds is to elucidate the disease mechanisms and evaluate the outcomes of therapeutic interventions. However, this can be quite difficult due to various matrix effects and the problems of chromatographic separation of analytes. In the current study; for the first time, an optimized and fully validated fully according to FDA and EMA Bioanalytical Validation Guidance HPLC-EC method was developed for the simultaneous analysis of nine neurotransmitter compounds which are dopamine (DA), homovanilic acid (HVA), vanilmandelic acid (VA) serotonin (SER), 5-hydroxyindole-3-acetic acid (5-HIAA), 4-hydroxy-3-methoxyphenylglycol (MHPG), norepinephrine (NE), 3,4 dihydroxyphenylacetic acid (DOPAC) and 3-methoxytyramine (3-MT) and simultaneously determined in rat brain samples. METHODS: The separation was achieved with 150 mm × 4.6 mm, 2.6 μm F5 Kinetex (Phenomenex, USA) column isocratically, and analysis was carried out HPLC equipped with DECADE II electrochemical detector. RESULTS: The method exhibited good selectivity and correlation coefficient values for each analyte’s calibration curves were >0.99. The detection and quantification limits ranged from 0.01 to 0.03 ng/mL and 3.04 to 9.13 ng/mL, respectively. The stability of the analytes and method robustness were also examined in detail in the study, and the obtained results are also presented statistically. DISCUSSION AND CONCLUSION: The developed fully validated method has been successfully applied to real rat brain samples and important results have been obtained. In the rat brain sample analysis, the least amount of SER and the highest amount of NA were found.","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45169243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advantages and Disadvantages of Two <i>In Vitro</i> Assays in Evaluating Aromatase Activity: \"A Cell-Based and a Cell-Free Assay\".","authors":"Elif İnce Ergüç,&nbsp;Senem Özcan Sezer,&nbsp;Hande Gürer Orhan","doi":"10.4274/tjps.galenos.2021.85530","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2021.85530","url":null,"abstract":"<p><strong>Objectives: </strong>Aromatase is an enzyme that catalyzes the conversion of androgens to estrogens. While inhibition of aromatase is a useful approach for treating breast cancer, it may also have toxicological consequences due to its endocrine disrupting/modulating effect. In this study, sensitivity and performance of two <i>in vitro</i> assays -a cell free and a cell-based- for evaluating aromatase activity were investigated by testing known aromatase inhibitors and partial validation of the methods was performed. Advantages and disadvantages of these methods are also discussed.</p><p><strong>Materials and methods: </strong>Aromatase activity was evaluated <i>via</i> two <i>in vitro</i> models; direct measurement with a cell-free assay using a fluorescent substrate and recombinant human enzyme and indirect evaluation with a cell-based assay where cell proliferation was determined in estrogen receptor positive human breast cancer cells (MCF-7 BUS) in the absence of estrogen and the presence of testosterone.</p><p><strong>Results: </strong>In the cell-free direct measurement assay, reference compounds ketoconazole and aminoglutethimide have been shown to inhibit the aromatase enzyme with half-maximal inhibitory concentration (IC₅₀) values concordant with literature. In cell-based indirect measurement assay, only ketoconazole dose-dependently inhibited cell proliferation with 3.47 x 10^-7 M IC₅₀. Inter-assay and intra-assay reproducibility of both methods was found to be within acceptable deviation levels.</p><p><strong>Conclusion: </strong>Both methods can be successfully applied. However, to evaluate the potential aromatase activity of the novel compounds <i>in vitro</i>, it seems better to perform both the cell-based and the cell-free assays that allows low-moderate biotransformation and eliminate cytotoxicity potential, respectively.</p>","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":"19 6","pages":"626-629"},"PeriodicalIF":1.7,"publicationDate":"2022-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9780571/pdf/TJPS-19-626.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10799612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}