Turkish Journal of Biology最新文献_第9页

Induction of haploid plants in citrus through gamma-irradiated pollen and ascertainment of ovule age for maximum recovery of haploid plantlets 利用γ辐照花粉诱导柑橘单倍体植株及确定胚珠龄以最大限度恢复单倍体植株

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-06-01 DOI: 10.3906/BIY-1606-28

M. Kundu, A. Dubey, M. Srivastav, S. Malik

引用次数: 8

Morphology, development, and transplant potential of Prunus avium and Cornus sanguinea seedlings growing under different LED lights 不同LED灯下桃李和血茱萸幼苗的形态、发育和移植潜力

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-04-20 DOI: 10.3906/BIY-1607-19

F. Bantis, K. Radoglou

引用次数: 12

Improvement in androgenic response of borage (Borago officinalis L.) cultured anthers using antibrowning agents and picloram 抗褐变剂和picloram对琉璃苣培养花药雄激素反应的改善

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-04-20 DOI: 10.3906/BIY-1606-49

M. Abdollahi, Zahra Chardoli Eshaghi, M. Majdi

引用次数: 5

Optimization of monoclonal antibody expression in CHOcells by employing epigenetic gene regulation tools 应用表观遗传学基因调控工具优化CHO细胞单克隆抗体表达

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-04-02 DOI: 10.3906/biy-1702-18

Fatemeh Nematpour, F. Mahboudi, V. Khalaj, B. Vaziri, S. Ahmadi, M. Ahmadi, Saedeh Ebadat, Fatemeh Davami

{"title":"Optimization of monoclonal antibody expression in CHOcells by employing epigenetic gene regulation tools","authors":"Fatemeh Nematpour, F. Mahboudi, V. Khalaj, B. Vaziri, S. Ahmadi, M. Ahmadi, Saedeh Ebadat, Fatemeh Davami","doi":"10.3906/biy-1702-18","DOIUrl":"https://doi.org/10.3906/biy-1702-18","url":null,"abstract":"Monoclonal antibodies (mAbs) are crucial in pharmaceutical biotechnology. Mammalian cell lines are the most preferred for their production. One of the significant challenging issues of mammalian expression systems is epigenetic gene silencing. Employing epigenetic gene regulation tools can increase the productivity of the mammalian cell lines. Sodium butyrate (NaBut) and valproic acid (VPA) regulate gene expression by inhibiting histone deacetylase. A ubiquitous chromatin-opening element (UCOE) can improve expression by reducing DNA methylation. Here, the separate and combined effects of NaBut and VPA histone deacetylase inhibitors (iHDACs) and UCOE on mAb synthesis were studied. Stable cell lines were generated by non-UCOE (CHO-HL) and UCOE-containing vectors (CHO-UHUL) and cultured in the presence and absence of NaBut or VPA. Expression analysis showed that CHO-UHUL gave a 4-fold greater yield than non-UCOE CHO-HL. Antibody production levels of the CHO-HL and CHO-UHUL cells increased 2-fold and 2.5-fold after NaBut and VPA treatment, respectively. These results indicate that UCOE has more impact on antibody expression than iHDACs. iHDAC treatment exhibited at least a 0.5-fold higher antibody yield in UCOE containing CHO-UHUL cells. Thus utilization of NaBut and VPA (iHDACs) and UCOE resulted in antibody expression improvement, and the combined use of them had a synergistic effect on antibody synthesis. \u0000Keywords: Monoclonal antibodies, Chinese hamster ovary, epigenetic gene silencing, chromatin-opening element, sodium butyrate, valproic acid","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2017-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/biy-1702-18","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46106713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Cytotoxic and apoptotic effects of endemic Centaurea fenzlii Reichardt on the MCF-7 breast cancer cell line 地方性半毛茛对MCF-7乳腺癌细胞系的细胞毒性和凋亡作用

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-04-01 DOI: 10.3906/biy-1609-74

Ümit Yırtıcı, Fatih Göger, Mehmet Sarimahmut, A. Ergene

{"title":"Cytotoxic and apoptotic effects of endemic Centaurea fenzlii Reichardt on the MCF-7 breast cancer cell line","authors":"Ümit Yırtıcı, Fatih Göger, Mehmet Sarimahmut, A. Ergene","doi":"10.3906/biy-1609-74","DOIUrl":"https://doi.org/10.3906/biy-1609-74","url":null,"abstract":"The main purpose of this study was to analyze the cytotoxic activity of an extract obtained from Centaurea fenzlii Reichardt, and the fractions eluted from this extract, in breast cancer cells. After isolation and structural analysis of the fractions were conducted, a meaningful cytotoxic effect was indicated. The goal of the analysis was to reveal the mechanism by which this effect occurs through researching the apoptotic side of these fractions and determining the amount of several proteins that are the products of the genes. Test substances were applied to breast cancer cells and the inhibitory concentration value 50 (IC50) that caused a cytotoxic effect was determined using MTT and ATP assays. The Centaurea fenzlii Reichardt dichloromethane extracts-ethyl acetate fractions (CFDCMEAF) exhibited a stronger growth-inhibitory effect on MCF-7 cells (45.771 μg/mL). The apoptotic effect was studied using double staining and flow cytometry. The death rate in the cells treated with the CFDCM-EAF IC50 dose was approximately 90%: 9.2% living cells, 22.8% necrotic cells, 62.3% late apoptotic cells, and 5.8% early apoptotic cells. Structural analysis of the CFDCM-EAF, which indicated significant cytotoxic effects, was performed using chromatographic methods. Hispidulin was the major component of the CFDCM-EAF by LC-APCI-MS/MS analysis.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/biy-1609-74","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42164662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative/antioxidative status 神经肽Y通过改变氧化/抗氧化状态来降低人绒毛膜癌细胞系的迁移能力

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-04-01 DOI: 10.3906/biy-1606-73

M. Matić, Milica G. Paunović, B. Ognjanović, Stajn As, Z. Saicic

{"title":"Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative/antioxidative status","authors":"M. Matić, Milica G. Paunović, B. Ognjanović, Stajn As, Z. Saicic","doi":"10.3906/biy-1606-73","DOIUrl":"https://doi.org/10.3906/biy-1606-73","url":null,"abstract":"Reduced migration capacity of trophoblast cells leads to poor placentation and correlates with severe pregnancy disorders such as intrauterine growth restriction and preeclampsia. Neuropeptide Y (NPY) is sympathetic cotransmitter involved in various physiological processes and its levels are significantly increased in preeclamptic pregnancy compared to healthy pregnancy. In this study the prooxidative role of NPY and its effects on migration capacity of human trophoblast cell line JEG-3 were investigated together with the effects of nitric oxide (NO) depletion, a molecule that was shown to play an important role in promoting cell migration. The cells were treated for 24 h (short-term stimulation) and 72 h (long-term stimulation) respectively with 1 nM NPY. Oxidative/antioxidative status and the migration index of cells were measured. The results showed increased concentrations of oxidative stress parameters (O2.-, H2O2) and molecules of the antioxidant defense system (reduced glutathione and oxidized glutathione), while the levels of intracellular nitrites (indicators of NO) and cell migration index were significantly decreased in trophoblast cells treated with NPY (both at 24 h and 72 h of exposure) compared to the control cells. These results suggest that NPY may significantly contribute to reduced migration capacity of trophoblast cells by generating oxidative stress and reducing the bioavailability of NO.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/biy-1606-73","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41300191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Biocompatible polymeric coatings do not inherently reduce the cytotoxicity of iron oxide nanoparticles 生物相容性聚合物涂层本身并不会降低氧化铁纳米颗粒的细胞毒性

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-04-01 DOI: 10.3906/BIY-1608-61

A. Ş. Ürkmez, Ece Bayir, E. Bilgi, M. Ozen

{"title":"Biocompatible polymeric coatings do not inherently reduce the cytotoxicity of iron oxide nanoparticles","authors":"A. Ş. Ürkmez, Ece Bayir, E. Bilgi, M. Ozen","doi":"10.3906/BIY-1608-61","DOIUrl":"https://doi.org/10.3906/BIY-1608-61","url":null,"abstract":"Nanotechnology in biomedical research is an emerging and promising tool for different purposes, like high-resolution medical imaging, diagnostics, and targeted drug delivery. Although some experimental efforts focused on determination of the cytotoxicity of nanoparticles (NPs) are present, there are many controversial results. In this study, chitosan (CS)- and poly(acrylic acid) (PAA)-coated iron oxide nanoparticles (IONPs) were used to investigate the possible cytotoxicity of coated IONPs on model mammalian cell line SaOs-2 by evaluating cellular viability and membrane integrity. Increasing concentrations of IONPs increased the cytotoxic effect on SaOs-2 cells with both CS- and PAA-coated IONPs. Cell viability on day 3 was as low as 40% and 48% at 1000 ?M concentration for PAAand CS-coated IONPs, respectively, in 1% FBS-supplemented media. Cytotoxicity determined by the released lactate dehydrogenase (LDH) was as high as 163% with 1000 ?M concentration of CS-IONPs, while there was no significant change in LDH release with PAAcoated IONPs at any concentration. This study reveals that IONPs coated with a biocompatible polymer, which are usually assumed to be nontoxic, show cytotoxicity with increasing concentration and incubation time. The cytotoxicity of nanoparticles intended for biomedical purposes must be evaluated using more than one approach.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1608-61","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43937261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Amperometric biosensor for detection of triglyceride tributyrinbased on zero point charge of activated carbon 基于活性炭零点电荷的三丁酸甘油三酯检测的安培生物传感器

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-04-01 DOI: 10.3906/BIY-1607-24

Mohsen Rezvani, G. Najafpour, M. Mohammadi, H. Zare

{"title":"Amperometric biosensor for detection of triglyceride tributyrinbased on zero point charge of activated carbon","authors":"Mohsen Rezvani, G. Najafpour, M. Mohammadi, H. Zare","doi":"10.3906/BIY-1607-24","DOIUrl":"https://doi.org/10.3906/BIY-1607-24","url":null,"abstract":"A simple and robust single enzyme biosensor was fabricated for triglyceride (TG) detection. Graphite rods were modified with activated carbon (AC) and used as support for lipase immobilization. Chitosan (CHIT) was eventually used to create film on the bioelectrode and retain the immobilized enzyme. To extend the linear range of TG detection, AC was functionalized with carboxyl and then amine groups (AAC) to enhance the isoelectric point of AC. The constructed graphite/AAC/lipase /CHIT bioelectrode was characterized using cyclic voltammetry and field emission scanning electron microscopy (FE-SEM). The accuracy of the developed biosensor was assessed through determination of different concentrations of tributyrin (TB) in buffer solution. Linear responses were found for TB concentration in the range of 50 to 350 mg dL-1 with a detection limit of 9.9 mg dL-1. The biosensor showed good sensitivity of 0.16 μA mg-1 dL. The detected level of TG in several human serum specimens using the constructed biosensor was in good agreement with the results of an automatic biochemical analyzer. The fabricated biosensor was not affected by a number of human serum materials and showed a strong anti-interference ability. The relative standard deviation in reproducibility and repeatability tests was 3.46% and 2.94%, respectively.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1607-24","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48401816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

MicroRNA-34a regulates brain-derived neurotrophic factor in an intracerebral hemorrhage model MicroRNA-34a在脑出血模型中调控脑源性神经营养因子

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-04-01 DOI: 10.3906/BIY-1606-16

Jin Hee Kim, Jae-Sun Choi

{"title":"MicroRNA-34a regulates brain-derived neurotrophic factor in an intracerebral hemorrhage model","authors":"Jin Hee Kim, Jae-Sun Choi","doi":"10.3906/BIY-1606-16","DOIUrl":"https://doi.org/10.3906/BIY-1606-16","url":null,"abstract":"Intracerebral hemorrhages (ICHs) are devastating neurological events frequently resulting in a serious negative prognosis. The exact physiological and disease processes involved in ICHs are complex, but are thought to involve microRNAs (miRNAs), 22 nucleotide small noncoding RNAs that control a variety of normal physiological and disease processes. In this study, we show that a miRNA, miR-34a, regulates BDNF in a model of ICH injury. In particular, we assessed the impact of AM34a, an inhibitor of miR-34a, on the toxicity of thrombin-induced apoptosis and on BDNF-mediated signaling. We investigated the increased expression of miR-34a after an ICH-induced thrombin toxicity injury using a real-time polymerase chain reaction (RT-PCR) and evaluated miR-34a as a therapeutic target. Apoptosis was confirmed by 4',6-diamidino-2-phenylindole using terminal deoxynucleotidyl transferase-mediated digoxigenindUTP- biotin nick-end labeling (TUNEL). The number of apoptotic cells detected by TUNEL after ICH injury was decreased by AM34a. Additionally, the ICH injury model treated with AM34a had a significantly lower caspase-3 level. We performed western blot analyses for BDNF, phosphorylated Akt, and phosphorylated ERK. The levels of BDNF were significantly higher in samples treated with AM34a. Furthermore, the level of phosphorylated Akt and phosphorylated ERK were significantly higher under AM34a. In conclusion, we demonstrated a distinct miRNA expression pattern after an in vitro ICH injury model, and modulation of this pattern can have therapeutic potential. miR-34a antagomir reduced cell death and enhanced neurological recovery by activating the BDNF pro-survival pathway. This suggests that inhibiting miR-34a might be a potential therapeutic target in ICH.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1606-16","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47218002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Heterologous expression and characterization of a high redox potential laccase from Coriolopsis polyzona MUCL 38443 多带毛桑MUCL 38443高氧化还原电位漆酶的异源表达及特性研究

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-04-01 DOI: 10.3906/BIY-1605-51

O. Pinar, Candan Tamerler Behar, A. Karataş

{"title":"Heterologous expression and characterization of a high redox potential laccase from Coriolopsis polyzona MUCL 38443","authors":"O. Pinar, Candan Tamerler Behar, A. Karataş","doi":"10.3906/BIY-1605-51","DOIUrl":"https://doi.org/10.3906/BIY-1605-51","url":null,"abstract":"In this study, a novel laccase gene, named as Cplcc1, and its corresponding cDNA were isolated and characterized from the Coriolopsis polyzona MUCL 38443 strain. The Cplcc1 gene consists of a 1563-bp open reading frame encoding a protein of 520 amino acids with a 20-residue putative signal peptide. The size of the Cplcc1 gene is 2106 bp and it contains ten introns and five potential N-glycosylation sites. Additionally, the isolated full-length Cplcc1 cDNA was successfully expressed in Pichia pastoris. The heterologous expression conditions were also optimized and the highest activity value increased to 800 U L-1 with 1.5% methanol, 0.8 mM CuSO4, and 0.6% L-alanine supplementation. The recombinant laccase was partially purified and the molecular weight was found as approximately 54 kDa. The maximum oxidation activity was observed for 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) at pH 3.0. The optimal temperature was found as 70 °C. On the other hand, at 30 °C, the enzyme was stable for more than a week and its half-life was longer than 8 h. The Km, Vmax, kcat, and kcat Km-1 values of the recombinant laccase were identified as 0.137 mM, 288.6 μmol min-1 L-1, 5.73 x 105 min-1, and 4.18 x 106 min-1 mM-1, respectively. Sodium azide, L-cysteine, and SDS were found as usual inhibitors.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1605-51","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70173556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11