Turkish Journal of Biology最新文献_第9页

MicroRNA-34a regulates brain-derived neurotrophic factor in an intracerebral hemorrhage model MicroRNA-34a在脑出血模型中调控脑源性神经营养因子

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-04-01 DOI: 10.3906/BIY-1606-16

Jin Hee Kim, Jae-Sun Choi

{"title":"MicroRNA-34a regulates brain-derived neurotrophic factor in an intracerebral hemorrhage model","authors":"Jin Hee Kim, Jae-Sun Choi","doi":"10.3906/BIY-1606-16","DOIUrl":"https://doi.org/10.3906/BIY-1606-16","url":null,"abstract":"Intracerebral hemorrhages (ICHs) are devastating neurological events frequently resulting in a serious negative prognosis. The exact physiological and disease processes involved in ICHs are complex, but are thought to involve microRNAs (miRNAs), 22 nucleotide small noncoding RNAs that control a variety of normal physiological and disease processes. In this study, we show that a miRNA, miR-34a, regulates BDNF in a model of ICH injury. In particular, we assessed the impact of AM34a, an inhibitor of miR-34a, on the toxicity of thrombin-induced apoptosis and on BDNF-mediated signaling. We investigated the increased expression of miR-34a after an ICH-induced thrombin toxicity injury using a real-time polymerase chain reaction (RT-PCR) and evaluated miR-34a as a therapeutic target. Apoptosis was confirmed by 4',6-diamidino-2-phenylindole using terminal deoxynucleotidyl transferase-mediated digoxigenindUTP- biotin nick-end labeling (TUNEL). The number of apoptotic cells detected by TUNEL after ICH injury was decreased by AM34a. Additionally, the ICH injury model treated with AM34a had a significantly lower caspase-3 level. We performed western blot analyses for BDNF, phosphorylated Akt, and phosphorylated ERK. The levels of BDNF were significantly higher in samples treated with AM34a. Furthermore, the level of phosphorylated Akt and phosphorylated ERK were significantly higher under AM34a. In conclusion, we demonstrated a distinct miRNA expression pattern after an in vitro ICH injury model, and modulation of this pattern can have therapeutic potential. miR-34a antagomir reduced cell death and enhanced neurological recovery by activating the BDNF pro-survival pathway. This suggests that inhibiting miR-34a might be a potential therapeutic target in ICH.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":"41 1","pages":"249-255"},"PeriodicalIF":2.2,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1606-16","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47218002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Amperometric biosensor for detection of triglyceride tributyrinbased on zero point charge of activated carbon 基于活性炭零点电荷的三丁酸甘油三酯检测的安培生物传感器

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-04-01 DOI: 10.3906/BIY-1607-24

Mohsen Rezvani, G. Najafpour, M. Mohammadi, H. Zare

{"title":"Amperometric biosensor for detection of triglyceride tributyrinbased on zero point charge of activated carbon","authors":"Mohsen Rezvani, G. Najafpour, M. Mohammadi, H. Zare","doi":"10.3906/BIY-1607-24","DOIUrl":"https://doi.org/10.3906/BIY-1607-24","url":null,"abstract":"A simple and robust single enzyme biosensor was fabricated for triglyceride (TG) detection. Graphite rods were modified with activated carbon (AC) and used as support for lipase immobilization. Chitosan (CHIT) was eventually used to create film on the bioelectrode and retain the immobilized enzyme. To extend the linear range of TG detection, AC was functionalized with carboxyl and then amine groups (AAC) to enhance the isoelectric point of AC. The constructed graphite/AAC/lipase /CHIT bioelectrode was characterized using cyclic voltammetry and field emission scanning electron microscopy (FE-SEM). The accuracy of the developed biosensor was assessed through determination of different concentrations of tributyrin (TB) in buffer solution. Linear responses were found for TB concentration in the range of 50 to 350 mg dL-1 with a detection limit of 9.9 mg dL-1. The biosensor showed good sensitivity of 0.16 μA mg-1 dL. The detected level of TG in several human serum specimens using the constructed biosensor was in good agreement with the results of an automatic biochemical analyzer. The fabricated biosensor was not affected by a number of human serum materials and showed a strong anti-interference ability. The relative standard deviation in reproducibility and repeatability tests was 3.46% and 2.94%, respectively.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":"41 1","pages":"268-277"},"PeriodicalIF":2.2,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1607-24","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48401816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Heterologous expression and characterization of a high redox potential laccase from Coriolopsis polyzona MUCL 38443 多带毛桑MUCL 38443高氧化还原电位漆酶的异源表达及特性研究

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-04-01 DOI: 10.3906/BIY-1605-51

O. Pinar, Candan Tamerler Behar, A. Karataş

{"title":"Heterologous expression and characterization of a high redox potential laccase from Coriolopsis polyzona MUCL 38443","authors":"O. Pinar, Candan Tamerler Behar, A. Karataş","doi":"10.3906/BIY-1605-51","DOIUrl":"https://doi.org/10.3906/BIY-1605-51","url":null,"abstract":"In this study, a novel laccase gene, named as Cplcc1, and its corresponding cDNA were isolated and characterized from the Coriolopsis polyzona MUCL 38443 strain. The Cplcc1 gene consists of a 1563-bp open reading frame encoding a protein of 520 amino acids with a 20-residue putative signal peptide. The size of the Cplcc1 gene is 2106 bp and it contains ten introns and five potential N-glycosylation sites. Additionally, the isolated full-length Cplcc1 cDNA was successfully expressed in Pichia pastoris. The heterologous expression conditions were also optimized and the highest activity value increased to 800 U L-1 with 1.5% methanol, 0.8 mM CuSO4, and 0.6% L-alanine supplementation. The recombinant laccase was partially purified and the molecular weight was found as approximately 54 kDa. The maximum oxidation activity was observed for 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) at pH 3.0. The optimal temperature was found as 70 °C. On the other hand, at 30 °C, the enzyme was stable for more than a week and its half-life was longer than 8 h. The Km, Vmax, kcat, and kcat Km-1 values of the recombinant laccase were identified as 0.137 mM, 288.6 μmol min-1 L-1, 5.73 x 105 min-1, and 4.18 x 106 min-1 mM-1, respectively. Sodium azide, L-cysteine, and SDS were found as usual inhibitors.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":"41 1","pages":"278-291"},"PeriodicalIF":2.2,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1605-51","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70173556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Genetic engineering of an industrial strain of Streptomyces clavuligerus for further enhancement of clavulanic acid production 为进一步提高棒草酸产量而对棒草链霉菌工业菌株的基因工程研究

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-04-01 DOI: 10.3906/BIY-1608-17

A. Kizildogan, G. Jaccard, Alper Mutlu, Ibrahim Sertdemir, G. Özcengiz

{"title":"Genetic engineering of an industrial strain of Streptomyces clavuligerus for further enhancement of clavulanic acid production","authors":"A. Kizildogan, G. Jaccard, Alper Mutlu, Ibrahim Sertdemir, G. Özcengiz","doi":"10.3906/BIY-1608-17","DOIUrl":"https://doi.org/10.3906/BIY-1608-17","url":null,"abstract":"An industrial clavulanic acid (CA) overproducer Streptomyces clavuligerus strain, namely DEPA, was engineered to further enhance its CA production. Single or multiple copies of ccaR, claR (pathway-specific activators), and cas2 (CA synthase) genes under the control of different promoters were introduced into this strain. CA titers of the resulting recombinants were analyzed by HPLC in a dynamic fashion and compared to the vector-only controls and a wild-type strain of S. clavuligerus while their growth was monitored throughout fermentation. The addition of an extra copy of ccaR, under control of its own promoter or constitutive ermE* promoter (PermE*), led to 7.6- and 2.3-fold increased volumetric levels of CA in respective recombinants, namely the AK9 and ID3 strains. Its highly stable multicopy expression by the glpF promoter (PglpF) provided up to 25.9-fold enhanced volumetric CA titers in the respective recombinant, IDG3. claR expression controlled with its own promoter or ermE* and glpF-mediated amplification in an industrial strain brought about only about 1.2-fold increase in the volumetric CA titers. An extra copy of cas2 integration with PermE* into the S. clavuligerus DEPA genome led to 3.8-fold higher volumetric CA production by GV61. Conclusively, multicopy expression of ccaR under PglpF can result in significantly improved industrial high-titer CA producers.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":"41 1","pages":"342-353"},"PeriodicalIF":2.2,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1608-17","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48300936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Population variation in drought resistance and its relationship with adaptive and physiological seedling traits in Turkish red pine (Pinus brutia Ten.) 土耳其红松(Pinus brutia Ten.)抗旱性种群变异及其与幼苗适应生理性状的关系

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-04-01 DOI: 10.3906/BIY-1608-77

G. Kandemir, S. Önde, F. Temel, Z. Kaya

{"title":"Population variation in drought resistance and its relationship with adaptive and physiological seedling traits in Turkish red pine (Pinus brutia Ten.)","authors":"G. Kandemir, S. Önde, F. Temel, Z. Kaya","doi":"10.3906/BIY-1608-77","DOIUrl":"https://doi.org/10.3906/BIY-1608-77","url":null,"abstract":"Variation in drought resistance and its relationship with adaptive and physiological traits in forest trees are important in choosing suitable seed sources for reforestation and afforestation programs. A common garden experiment using 240 half-sib families originating from coastal and inland populations of Turkish red pine (Pinus brutia) in Turkey was set up with three replicates. The aims were to determine variation of drought damage, height growth, and phenology among populations and to investigate the relationship between drought damage and physiological traits (i.e. plant moisture stress and proline content). Three-year-old seedlings were subjected to drought treatment during the summer of 2000 and adaptive and physiological traits were measured. Except for bud burst, the majority of the variation resided between populations, leading to low heritability estimates for all traits. On average, inland populations were more resistant to drought and taller, with earlier bud burst and bud set times, than coastal populations. Proline content increased with higher drought damage, especially in cold-resistant and inland families. Inland populations are more drought-resistant than coastal populations. The results of the study demonstrate the possibility of selection for drought resistance for Turkish red pine at the population level.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":"41 1","pages":"256-267"},"PeriodicalIF":2.2,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1608-77","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47464758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

The comparison of antioxidant capacity and cytotoxic, anticarcinogenic, and genotoxic effects of Fe@Au nanosphere magnetic nanoparticles Fe@Au纳米球形磁性纳米颗粒的抗氧化能力、细胞毒性、抗癌和基因毒性作用的比较

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-04-01 DOI: 10.3906/biy-1607-2

Hande Yeğenoğlu, B. Aslim, Burcu Guven, A. Zengin, I. Boyaci, Z. Suludere, U. Tamer

{"title":"The comparison of antioxidant capacity and cytotoxic, anticarcinogenic, and genotoxic effects of Fe@Au nanosphere magnetic nanoparticles","authors":"Hande Yeğenoğlu, B. Aslim, Burcu Guven, A. Zengin, I. Boyaci, Z. Suludere, U. Tamer","doi":"10.3906/biy-1607-2","DOIUrl":"https://doi.org/10.3906/biy-1607-2","url":null,"abstract":"Magnetic gold nanoparticles are used in various biomedical, biochemistry, and biotechnology applications due to their controllable size distribution, long-term stability, reduced toxicity, and biocompatibility. Different coating materials, such as proteins, carbohydrates, lipids, and polyphenols, are applied to enhance the biocompatibility of nanoparticles. In this study, the effects of surface coatings of core-shell structured Fe@Au nanosphere magnetic nanoparticles with regard to antioxidant capacity and cytotoxic, anticarcinogenic, and genotoxic properties were investigated. The obtained results demonstrated that avidin-coated Fe@Au nanospheres had higher antioxidant capacities than uncoated nanospheres. Neither avidin-coated nor uncoated nanoparticles had a cytotoxic effect on normal cells (human gingival fibroblast cell line, HGF-1). In addition, they had anticarcinogenic effects on human cervical carcinoma (HeLa), human breast adenocarcinoma (MCF-7), and human colorectal adenocarcinoma (CCL-221). The genotoxic effects of nanoparticles were also evaluated with DNA tail damage ratio.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":"41 1","pages":"302-313"},"PeriodicalIF":2.2,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41663630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

FRET-based characterization of K264A, D345A, and Y335Amutants in the human dopamine transporter 基于FRET的人类多巴胺转运蛋白中K264A、D345A和Y335 Amutants的表征

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-04-01 DOI: 10.3906/BIY-1607-9

O. Orun, P. Tiber

{"title":"FRET-based characterization of K264A, D345A, and Y335Amutants in the human dopamine transporter","authors":"O. Orun, P. Tiber","doi":"10.3906/BIY-1607-9","DOIUrl":"https://doi.org/10.3906/BIY-1607-9","url":null,"abstract":"The dopamine transporter (DAT) plays a role in the termination of dopaminergic neurotransmission; thereby it is accepted as the primary target of various psychostimulants. N-terminal phosphorylation of DAT has been proposed as a regulator in different DAT functions, such as amphetamine-induced efflux or PKC/PKA-mediated responses. To understand the role of N-terminal conformational changes in dopamine transporter structure and function, the fluorescence resonance energy transfer (FRET) method was applied to various DAT constructs fluorescently labeled at the N-terminus and substrate-induced conformational changes were determined using rhodamine-labeled cocaine analog JHC1-64 in three DAT mutants. The results indicated that the construct with YFP inserted into the N-terminal position-55 displayed effective interaction with the substrate and simultaneous mutation of two serine residues (S7 and S12) to alanine or aspartic acid demonstrated similar phenotypes as their wild-type (WT) counterparts. FRET was detectable for N-terminal p55 YFP WT, Ser/Ala, and Ser/Asp forms, but there was no significant difference among the three mutants, contrary to our expectations based on the previously proposed roles of these serine residues. In addition, three mutants (K264A, D345A, and Y335A) implemented in the position-55 YFP background were also investigated and the importance of Y335 in the translocation cycle and in the process of substrate release was verified.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":"41 1","pages":"378-387"},"PeriodicalIF":2.2,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1607-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48261635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The application of mesenchymal progenitor stem cells for the reduction of oxidative stress in animals 间充质祖细胞在降低动物氧化应激中的应用

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-02-20 DOI: 10.3906/BIY-1603-13

Edyta Wojtaś, A. Zachwieja, Anna Zwyrzykowska-Wodzińska, R. Kupczyński, K. Marycz

引用次数: 6

The colonization of Bacilllus thuringiensis strains in bryophytes 苏云金芽孢杆菌在苔藓植物中的定植

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-02-20 DOI: 10.3906/BIY-1510-16

Q. Lin, Peng Zhu, R. Carballar-Lejarazú, I. Gelbič, X. Guan, Lei Xu, Lingling Zhang

引用次数: 4

Identification of proteins differentially accumulated in Enterococcus faecalis under acrylamide exposure 丙烯酰胺暴露下粪肠球菌差异积累蛋白的鉴定