Turkish Journal of Biology最新文献_第10页

Genetic engineering of an industrial strain of Streptomyces clavuligerus for further enhancement of clavulanic acid production 为进一步提高棒草酸产量而对棒草链霉菌工业菌株的基因工程研究

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-04-01 DOI: 10.3906/BIY-1608-17

A. Kizildogan, G. Jaccard, Alper Mutlu, Ibrahim Sertdemir, G. Özcengiz

{"title":"Genetic engineering of an industrial strain of Streptomyces clavuligerus for further enhancement of clavulanic acid production","authors":"A. Kizildogan, G. Jaccard, Alper Mutlu, Ibrahim Sertdemir, G. Özcengiz","doi":"10.3906/BIY-1608-17","DOIUrl":"https://doi.org/10.3906/BIY-1608-17","url":null,"abstract":"An industrial clavulanic acid (CA) overproducer Streptomyces clavuligerus strain, namely DEPA, was engineered to further enhance its CA production. Single or multiple copies of ccaR, claR (pathway-specific activators), and cas2 (CA synthase) genes under the control of different promoters were introduced into this strain. CA titers of the resulting recombinants were analyzed by HPLC in a dynamic fashion and compared to the vector-only controls and a wild-type strain of S. clavuligerus while their growth was monitored throughout fermentation. The addition of an extra copy of ccaR, under control of its own promoter or constitutive ermE* promoter (PermE*), led to 7.6- and 2.3-fold increased volumetric levels of CA in respective recombinants, namely the AK9 and ID3 strains. Its highly stable multicopy expression by the glpF promoter (PglpF) provided up to 25.9-fold enhanced volumetric CA titers in the respective recombinant, IDG3. claR expression controlled with its own promoter or ermE* and glpF-mediated amplification in an industrial strain brought about only about 1.2-fold increase in the volumetric CA titers. An extra copy of cas2 integration with PermE* into the S. clavuligerus DEPA genome led to 3.8-fold higher volumetric CA production by GV61. Conclusively, multicopy expression of ccaR under PglpF can result in significantly improved industrial high-titer CA producers.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1608-17","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48300936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Population variation in drought resistance and its relationship with adaptive and physiological seedling traits in Turkish red pine (Pinus brutia Ten.) 土耳其红松(Pinus brutia Ten.)抗旱性种群变异及其与幼苗适应生理性状的关系

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-04-01 DOI: 10.3906/BIY-1608-77

G. Kandemir, S. Önde, F. Temel, Z. Kaya

{"title":"Population variation in drought resistance and its relationship with adaptive and physiological seedling traits in Turkish red pine (Pinus brutia Ten.)","authors":"G. Kandemir, S. Önde, F. Temel, Z. Kaya","doi":"10.3906/BIY-1608-77","DOIUrl":"https://doi.org/10.3906/BIY-1608-77","url":null,"abstract":"Variation in drought resistance and its relationship with adaptive and physiological traits in forest trees are important in choosing suitable seed sources for reforestation and afforestation programs. A common garden experiment using 240 half-sib families originating from coastal and inland populations of Turkish red pine (Pinus brutia) in Turkey was set up with three replicates. The aims were to determine variation of drought damage, height growth, and phenology among populations and to investigate the relationship between drought damage and physiological traits (i.e. plant moisture stress and proline content). Three-year-old seedlings were subjected to drought treatment during the summer of 2000 and adaptive and physiological traits were measured. Except for bud burst, the majority of the variation resided between populations, leading to low heritability estimates for all traits. On average, inland populations were more resistant to drought and taller, with earlier bud burst and bud set times, than coastal populations. Proline content increased with higher drought damage, especially in cold-resistant and inland families. Inland populations are more drought-resistant than coastal populations. The results of the study demonstrate the possibility of selection for drought resistance for Turkish red pine at the population level.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1608-77","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47464758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

The comparison of antioxidant capacity and cytotoxic, anticarcinogenic, and genotoxic effects of Fe@Au nanosphere magnetic nanoparticles Fe@Au纳米球形磁性纳米颗粒的抗氧化能力、细胞毒性、抗癌和基因毒性作用的比较

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-04-01 DOI: 10.3906/biy-1607-2

Hande Yeğenoğlu, B. Aslim, Burcu Guven, A. Zengin, I. Boyaci, Z. Suludere, U. Tamer

{"title":"The comparison of antioxidant capacity and cytotoxic, anticarcinogenic, and genotoxic effects of Fe@Au nanosphere magnetic nanoparticles","authors":"Hande Yeğenoğlu, B. Aslim, Burcu Guven, A. Zengin, I. Boyaci, Z. Suludere, U. Tamer","doi":"10.3906/biy-1607-2","DOIUrl":"https://doi.org/10.3906/biy-1607-2","url":null,"abstract":"Magnetic gold nanoparticles are used in various biomedical, biochemistry, and biotechnology applications due to their controllable size distribution, long-term stability, reduced toxicity, and biocompatibility. Different coating materials, such as proteins, carbohydrates, lipids, and polyphenols, are applied to enhance the biocompatibility of nanoparticles. In this study, the effects of surface coatings of core-shell structured Fe@Au nanosphere magnetic nanoparticles with regard to antioxidant capacity and cytotoxic, anticarcinogenic, and genotoxic properties were investigated. The obtained results demonstrated that avidin-coated Fe@Au nanospheres had higher antioxidant capacities than uncoated nanospheres. Neither avidin-coated nor uncoated nanoparticles had a cytotoxic effect on normal cells (human gingival fibroblast cell line, HGF-1). In addition, they had anticarcinogenic effects on human cervical carcinoma (HeLa), human breast adenocarcinoma (MCF-7), and human colorectal adenocarcinoma (CCL-221). The genotoxic effects of nanoparticles were also evaluated with DNA tail damage ratio.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41663630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

FRET-based characterization of K264A, D345A, and Y335Amutants in the human dopamine transporter 基于FRET的人类多巴胺转运蛋白中K264A、D345A和Y335 Amutants的表征

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-04-01 DOI: 10.3906/BIY-1607-9

O. Orun, P. Tiber

{"title":"FRET-based characterization of K264A, D345A, and Y335Amutants in the human dopamine transporter","authors":"O. Orun, P. Tiber","doi":"10.3906/BIY-1607-9","DOIUrl":"https://doi.org/10.3906/BIY-1607-9","url":null,"abstract":"The dopamine transporter (DAT) plays a role in the termination of dopaminergic neurotransmission; thereby it is accepted as the primary target of various psychostimulants. N-terminal phosphorylation of DAT has been proposed as a regulator in different DAT functions, such as amphetamine-induced efflux or PKC/PKA-mediated responses. To understand the role of N-terminal conformational changes in dopamine transporter structure and function, the fluorescence resonance energy transfer (FRET) method was applied to various DAT constructs fluorescently labeled at the N-terminus and substrate-induced conformational changes were determined using rhodamine-labeled cocaine analog JHC1-64 in three DAT mutants. The results indicated that the construct with YFP inserted into the N-terminal position-55 displayed effective interaction with the substrate and simultaneous mutation of two serine residues (S7 and S12) to alanine or aspartic acid demonstrated similar phenotypes as their wild-type (WT) counterparts. FRET was detectable for N-terminal p55 YFP WT, Ser/Ala, and Ser/Asp forms, but there was no significant difference among the three mutants, contrary to our expectations based on the previously proposed roles of these serine residues. In addition, three mutants (K264A, D345A, and Y335A) implemented in the position-55 YFP background were also investigated and the importance of Y335 in the translocation cycle and in the process of substrate release was verified.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1607-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48261635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The application of mesenchymal progenitor stem cells for the reduction of oxidative stress in animals 间充质祖细胞在降低动物氧化应激中的应用

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-02-20 DOI: 10.3906/BIY-1603-13

Edyta Wojtaś, A. Zachwieja, Anna Zwyrzykowska-Wodzińska, R. Kupczyński, K. Marycz

引用次数: 6

The colonization of Bacilllus thuringiensis strains in bryophytes 苏云金芽孢杆菌在苔藓植物中的定植

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-02-20 DOI: 10.3906/BIY-1510-16

Q. Lin, Peng Zhu, R. Carballar-Lejarazú, I. Gelbič, X. Guan, Lei Xu, Lingling Zhang

引用次数: 4

Identification of proteins differentially accumulated in Enterococcus faecalis under acrylamide exposure 丙烯酰胺暴露下粪肠球菌差异积累蛋白的鉴定

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-02-20 DOI: 10.3906/BIY-1606-23

A. Bocian, K. Hus, M. Jaromin, M. Tyrka, A. Lyskowski

引用次数: 3

Lipocalin gene expression is varied in developmental stages by larval nutritional stress in Drosophila 脂质体蛋白基因在果蝇发育阶段受幼虫营养应激的影响而变化

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-02-20 DOI: 10.3906/biy-1604-35

N. Ayhan, Pınar Güler, Banu Şebnem Önder

{"title":"Lipocalin gene expression is varied in developmental stages by larval nutritional stress in Drosophila","authors":"N. Ayhan, Pınar Güler, Banu Şebnem Önder","doi":"10.3906/biy-1604-35","DOIUrl":"https://doi.org/10.3906/biy-1604-35","url":null,"abstract":"Lipocalin genes NLaz, GLaz, and Karl are evolutionarily conserved genes in Drosophila melanogaster. There are studies on lipocalin gene expression differences under diverse diet conditions, but these studies have focused mainly on age-dependent expression profiles of these genes. The main aim of our study is to determine lipocalin expression in the developmental period by nutritional manipulation with an isofemale-based design. Three larval developmental periods have been researched under normal and restricted diets. We found significant differences between lines during their developmental time-related lipocalin expression. Here, we demonstrate that upregulations in the early developmental stages of lipocalin genes under stressful conditions resulted in unaffected developmental time. The possible reason for high expression is the activation of stress signal pathways in order to buffer the harmful effects of nutritional restriction. Our data showed that the early developmental period (48-72 h) is especially crucial to tolerate the dietary stress with respect to GLaz and NLaz expression. Results of this experiment have shown that the expression profiles of lipocalin genes have line-specific pathways to nutritional stress. Their expression depends on the genetic background corresponding to development time results. Our results highlight the transcriptional changes of lipocalins associated with developmental time in larvae, developed in a dietary-restricted medium.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2017-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/biy-1604-35","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43300852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of the function and expression pattern of auxin response factor B3 in the model legume plant Medicago truncatula 生长素反应因子B3在模式豆科植物截茎苜蓿中的功能和表达模式的评估

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-02-01 DOI: 10.3906/BIY-1602-36

M. Revalska, V. Vassileva, G. Zehirov, S. Goormachtig, A. Iantcheva

{"title":"Assessment of the function and expression pattern of auxin response factor B3 in the model legume plant Medicago truncatula","authors":"M. Revalska, V. Vassileva, G. Zehirov, S. Goormachtig, A. Iantcheva","doi":"10.3906/BIY-1602-36","DOIUrl":"https://doi.org/10.3906/BIY-1602-36","url":null,"abstract":"The phytohormone auxin is a critical signal molecule, regulating fundamental processes in plant growth and development, such as shaping the root and shoot architecture, organ patterning, and nodulation. Auxin regulates plant gene expression mainly through auxin response factors (ARFs), which bind to auxin response elements in the promoter, upstream of auxin-activated genes. Here we examine and assess the function and expression pattern of a gene described as an auxin response factor, containing a DNA-binding pseudobarrel and B3 DNA-binding domains, from Medicago truncatula (MtARF-B3). For the model legume species M. truncatula, stable transgenic plants with MtARF-B3 overexpression, downregulation, and transcriptional reporters were constructed. Phenotypic and morphological evaluation of the obtained transgenic plants confirmed the important role of MtARF-B3 in general plant growth and development, modeling of root architecture, and development of seeds. Detailed histochemical and transcriptional analysis revealed expression of the gene in various stages of somatic embryogenesis, during formation of plant organs and tissues, and symbiotic nodulation. The fact that MtARF-B3 was strongly expressed in stamens and pollen grains in M. truncatula suggests that this gene could play a role in the fertility of this model legume.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1602-36","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46349809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

The Sca1+ mesenchymal stromal subpopulation promotes dendritic cell commitment in the niche 鳞状+间充质基质亚群促进树突状细胞在生态位中的承诺

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2017-02-01 DOI: 10.3906/BIY-1510-81

Na Li, D. Yin, Huijie Zhang, Jinmei Xu, F. Wen, Zheng Liu, Yaozhen Chen, Ning An, Jiajia Xin, Yazhou Wang, Wen Yin, Xingbin Hu

{"title":"The Sca1+ mesenchymal stromal subpopulation promotes dendritic cell commitment in the niche","authors":"Na Li, D. Yin, Huijie Zhang, Jinmei Xu, F. Wen, Zheng Liu, Yaozhen Chen, Ning An, Jiajia Xin, Yazhou Wang, Wen Yin, Xingbin Hu","doi":"10.3906/BIY-1510-81","DOIUrl":"https://doi.org/10.3906/BIY-1510-81","url":null,"abstract":"The hematopoietic microenvironment regulates self-renewal and differentiation of hematopoietic stem cells. Mesenchymal stromal cells (MSCs) contribute to the niche and participate in supporting dendritic cell (DC) commitment in vitro and in vivo. However, due to MSCs being heterogenic, it is necessary to understand the function of the MSC subpopulation in modulating the DC commitment. The current study showed that one of the bone-related Sca1+ MSCs enhanced bone marrow cell differentiation into DCs by direct cell-to-cell contact. Furthermore, the expression of STAT3 and STAT5 genes decreased in supernatant cells after Sca1+ MSCs were cocultured with bone marrow cells. In contrast, the expression level of caspase-3 in cells increased. At the same time, the presentation of costimulatory molecules CD80/CD86 and the migration of activated DCs towards lymph nodes were augmented by Sca1+ MSCs. The results demonstrated that the Sca1+ MSC subpopulation promotes the differentiation of bone marrow cells into DCs by direct cell-to-cell contact via STAT3/STAT5 and caspase-3 signaling. Meanwhile, Sca1+ MSCs also mediate the activation and migration capabilities of DCs.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1510-81","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42709180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2