鳞状+间充质基质亚群促进树突状细胞在生态位中的承诺

IF 1.1 4区生物学 Q3 BIOLOGY

Turkish Journal of Biology Pub Date : 2017-02-01 DOI:10.3906/BIY-1510-81

Na Li, D. Yin, Huijie Zhang, Jinmei Xu, F. Wen, Zheng Liu, Yaozhen Chen, Ning An, Jiajia Xin, Yazhou Wang, Wen Yin, Xingbin Hu

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引用次数: 2

摘要

造血微环境调节造血干细胞的自我更新和分化。间充质基质细胞（MSCs）在体外和体内对小生境有贡献，并参与支持树突状细胞（DC）的结合。然而，由于MSC是异质性的，有必要了解MSC亚群在调节DC承诺中的功能。目前的研究表明，一种与骨相关的Sca1+MSCs通过直接的细胞间接触增强了骨髓细胞向DC的分化。此外，Sca1+MSC与骨髓细胞共培养后，上清液中STAT3和STAT5基因的表达降低。相反，细胞中胱天蛋白酶-3的表达水平增加。同时，Sca1+MSCs增强了共刺激分子CD80/CD86的呈递和活化的DC向淋巴结的迁移。结果表明，Sca1+MSC亚群通过STAT3/STAT5和胱天蛋白酶-3信号传导，通过细胞间直接接触，促进骨髓细胞分化为DC。同时，Sca1+MSCs也介导DC的激活和迁移能力。

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The Sca1+ mesenchymal stromal subpopulation promotes dendritic cell commitment in the niche

The hematopoietic microenvironment regulates self-renewal and differentiation of hematopoietic stem cells. Mesenchymal stromal cells (MSCs) contribute to the niche and participate in supporting dendritic cell (DC) commitment in vitro and in vivo. However, due to MSCs being heterogenic, it is necessary to understand the function of the MSC subpopulation in modulating the DC commitment. The current study showed that one of the bone-related Sca1+ MSCs enhanced bone marrow cell differentiation into DCs by direct cell-to-cell contact. Furthermore, the expression of STAT3 and STAT5 genes decreased in supernatant cells after Sca1+ MSCs were cocultured with bone marrow cells. In contrast, the expression level of caspase-3 in cells increased. At the same time, the presentation of costimulatory molecules CD80/CD86 and the migration of activated DCs towards lymph nodes were augmented by Sca1+ MSCs. The results demonstrated that the Sca1+ MSC subpopulation promotes the differentiation of bone marrow cells into DCs by direct cell-to-cell contact via STAT3/STAT5 and caspase-3 signaling. Meanwhile, Sca1+ MSCs also mediate the activation and migration capabilities of DCs.

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来源期刊

Turkish Journal of Biology BIOLOGY-

CiteScore

4.60

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The Turkish Journal of Biology is published electronically 6 times a year by the Scientific and Technological Research Council of Turkey (TÜBİTAK) and accepts English-language manuscripts concerning all kinds of biological processes including biochemistry and biosynthesis, physiology and metabolism, molecular genetics, molecular biology, genomics, proteomics, molecular farming, biotechnology/genetic transformation, nanobiotechnology, bioinformatics and systems biology, cell and developmental biology, stem cell biology, and reproductive biology. Contribution is open to researchers of all nationalities.