Turkish Journal of Biology最新文献_第3页

Knockdown of SET Domain, Bifurcated 1 suppresses head and neck cancer cell viability and wound-healing ability in vitro 敲除SET结构域，分叉1抑制头部和颈部癌症细胞的生存能力和伤口愈合能力

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2019-10-14 DOI: 10.3906/biy-1903-71

S. Özdaş

{"title":"Knockdown of SET Domain, Bifurcated 1 suppresses head and neck cancer cell viability and wound-healing ability in vitro","authors":"S. Özdaş","doi":"10.3906/biy-1903-71","DOIUrl":"https://doi.org/10.3906/biy-1903-71","url":null,"abstract":"Head and neck cancer (HNC) is the sixth most common cancer worldwide and therefore presents a global public health problem. There are no standard algorithms for the diagnosis and follow-up of the disease, and no effective current treatment approaches exist. Therefore, the discovery of new biomolecules and the design of new strategies to aid in early diagnosis is necessary, along with establishing prognostic factors of HNC. In several cancer studies, the upregulation of SET Domain, Bifurcated 1 (SETDB1) has been reported to be tumor-inducing and to indicate a cancer-invasive prognosis, leading to the modulation of genes associated with different signaling pathways; however, the literature is sparse regarding the relationship between SETDB1 and HNC. In our study, three HNC primary cell lines and their corresponding metastatic cell lines were used. The quantitative reverse transcriptase-polymerase chain reaction and western blotting data indicated that the SETDB1 mRNA and protein expression levels were higher in all metastatic cell lines compared to their primary cell lines (P < 0.05 for all). To investigate the role of SETDB1 in HNC biology, in vitro functional analyses were carried out using small interference RNA (siRNA) technology, cell viability, scratch wound-healing, and the caspase-3 activity assay of gene expression of SETDB1 to compare primary and metastatic cell lines of HNC. Metastatic cells were more susceptible to this suppression, which decreased the vitality of cells and their ability of wound-healing and induced level of caspase-3 activity (P < 0.05 for all). This functional study has shown that SETDB1 plays an important role in head and neck carcinogenesis. Therefore, SETDB1 may be an attractive therapeutic target molecule and also a potential diagnostic and prognostic biomarker in HNC.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2019-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/biy-1903-71","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44221259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

E2F6 is essential for cell viability in breast cancer cells during replication stress E2F6在复制应激期间对癌症乳腺细胞的细胞活力至关重要

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2019-10-14 DOI: 10.3906/biy-1905-6

I. J. Lafta

{"title":"E2F6 is essential for cell viability in breast cancer cells during replication stress","authors":"I. J. Lafta","doi":"10.3906/biy-1905-6","DOIUrl":"https://doi.org/10.3906/biy-1905-6","url":null,"abstract":"E2F6 is a member of the E2F family of transcription factors involved in regulation of a wide variety of genes through both activation and repression. E2F6 has been reported as overexpressed in breast cancers but whether or not this is important for tumor development is unclear. We first checked E2F6 expression in tumor cDNAs and the protein level in a range of breast cancer cell lines. RNA interference-mediated depletion was then used to assess the importance of E2F6 expression in cell lines with regard to cell cycle profile using fluorescence-activated cell sorting and a cell survival assay using (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). The overexpression of E2F6 was confirmed in breast tumor cDNA samples and breast cancer cell lines. Depletion of E2F6 in the breast cancer cells reduced cell viability in MCF-7, T-47D, and MDA-MB-231 cells. There was little effect in the nontumor breast cell line MCF-10A. The deleterious effect on cancer cells was greater during replication stress, leading to an increase in the proportion of breast cancer cells with sub-G1 DNA content. These results suggest that E2F6 might be essential for the survival of breast cancer cells experiencing replication stress, and therefore it could be a target for combined therapy.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2019-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/biy-1905-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49448948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Cytotoxic and genotoxic action of Tagetes patula flower methanol extract and patuletin using the Allium test 用葱试验研究万寿菊花甲醇提取物和球曲素的细胞毒和基因毒作用

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2019-10-14 DOI: 10.3906/biy-1906-7

M. Azhar, A. Farooq, S. Haque, S. Bano, Lubna Zaheer, S. Faizi

{"title":"Cytotoxic and genotoxic action of Tagetes patula flower methanol extract and patuletin using the Allium test","authors":"M. Azhar, A. Farooq, S. Haque, S. Bano, Lubna Zaheer, S. Faizi","doi":"10.3906/biy-1906-7","DOIUrl":"https://doi.org/10.3906/biy-1906-7","url":null,"abstract":"Tagetes patula is used to treat cancer patients in alternative healthcare systems. However, its cytotoxic and genotoxic effects have not been reported. Therefore, themethanol extract of T. patula flower, the ethyl acetate fraction, and the pure compound patuletin were evaluatedusing the Allium test.The methanol extract and fraction contained ~3% and ~36% patuletin, respectively, with ~98% purity. The methanol extract caused inhibition of Allium root growth displaying an IC50 value of ~500 µg/mL, while the fraction and patuletin were more potent by ~2 and ~5 times, respectively. The Allium root tips demonstrated a decline in prophase, metaphase, anaphase, and telophase stages with concomitant decrease in percent mitotic index in the methanol extract (~5.64), fraction, and patuletin (~4) as compared to the control (~7.61). However, in only methanol extract-treated root tips, an increase in metaphase stage was noted. In addition, the methanol extract predominantly induced c-type, misaligned, and multipolar chromosomal abnormalities while the fraction and patuletin displayed fragments and sticky chromosomes. The fraction and patuletin also produced micronuclei (~2%). In conclusion, T. patula flower methanol extract and ethyl acetate fraction are cytotoxicand genotoxic, which most likely could be due to the patuletin. Further preclinical and clinical studies are required to justify its clinical use.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2019-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44782292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

A new Helicoverpa armigera Nucleopolyhedrovirus isolate from Heliothis peltigera (Denis & Schiffermuller) (Lepidoptera: Noctuidae) in Turkey. 土耳其棉铃虫核多角体病毒新分离株(鳞翅目:夜蛾科)

IF 1.1 4区生物学

Turkish Journal of Biology Pub Date : 2019-10-14 eCollection Date: 2019-01-01 DOI: 10.3906/biy-1902-64

Gözde Büşra Eroğlu, Remziye Nalçacioğlu, Zihni Demirbağ

{"title":"A new Helicoverpa armigera Nucleopolyhedrovirus isolate from Heliothis peltigera (Denis & Schiffermuller) (Lepidoptera: Noctuidae) in Turkey.","authors":"Gözde Büşra Eroğlu, Remziye Nalçacioğlu, Zihni Demirbağ","doi":"10.3906/biy-1902-64","DOIUrl":"10.3906/biy-1902-64","url":null,"abstract":"This study reports a new Helicoverpa armigera nucleopolyhedrovirus (NPV) isolated from Heliothis peltigera (Denis & Schiffermuller), collected in the vicinity of Adana, Turkey. Infection was confirmed by tissue polymerase chain reaction and sequence analysis. Results showed that dead H. peltigera larvae contain Helicoverpa armigera nucleopolyhedrovirus. Thus, the isolate was named as HearNPV-TR. Microscopy studies indicated that occlusion bodies were 0.73 to 1.66 μm in diameter. The nucleocapsids are approximately 184 × 41 nm in size. The genome of HearNPV-TR was digested with KpnI and XhoI enzymes and calculated as 130.5 kb. Phylogenetic analysis showed that HearNPV-TR has close relation with the H. armigera SNPV-1073 China isolate. The Kimura analysis confirmed that the isolate is a variant of H. armigera NPV. Bioassays were performed using six different concentrations (1 × 103 to 1 × 108 occlusion bodies (OBs)/mL) on 2nd instar larvae of H. peltigera, H. armigera, Heliothis viriplaca, Heliothis nubigera. LC50 values were calculated to be 9.5 × 103, 1.9 × 104, 8.6 × 104 and 9.2 × 104 OBs/mL within 14 days, respectively. Results showed that it is a promising biocontrol agent against Heliothinae species.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2019-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6823914/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44942014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic and physiological responses in mangrove Kandelia candel roots under short-term high-salinity stress 短期高盐度胁迫下红树红烛根的蛋白质组学和生理响应

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2019-10-14 DOI: 10.3906/biy-1906-22

Jianhong Xing, Dezhuo Pan, Lingxia Wang, Fanglin Tan, Wei Chen

{"title":"Proteomic and physiological responses in mangrove Kandelia candel roots under short-term high-salinity stress","authors":"Jianhong Xing, Dezhuo Pan, Lingxia Wang, Fanglin Tan, Wei Chen","doi":"10.3906/biy-1906-22","DOIUrl":"https://doi.org/10.3906/biy-1906-22","url":null,"abstract":"Kandelia candel is one of the mangrove species that are most resistant to environmental stress. As a typical nonsalt-secreting mangrove plant, K. candel is an ideal biological material to analyze the molecular mechanism of salt tolerance in woody plants. In this study, changes in protein abundance and expression profile in K. candel roots under high-salinity stress of 600 mmol L-1 NaCl were analyzed using isobaric tags for relative and absolute quantification (iTRAQ) assay. Moreover, the physiological parameters associated with metabolic pathways in which the differentially abundant proteins (DAPs) are involved were determined. A total of 5577 proteins were identified by iTRAQ analysis of the K. candel root proteins, of which 227 were DAPs with a fold change ratio >1.2 or a fold change ratio <0.83 and a P-value <0.05. A total of 227 DAPs consisting of 110 up-regulated and 117 down-regulated proteins were identified. Our Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed that the DAPs were primarily involved in biological processes including carbohydrate and energy metabolisms, stress response and defense, cell wall structure, and secondary metabolism. The results of the physiological parameters showed that their profile changes were consistent with those of the proteome analysis. The results of the proteome and physiological parameters showed that K. candel roots could resist high-salinity stress by maintaining a normal Embden-Meyerhof-Parnas and tricarboxylic acid (EMP-TCA) pathway, increasing the activities of various antioxidant enzymes and antioxidant contents, stabilizing the cell wall structure, and accumulating secondary metabolites such as triterpenoids.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2019-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/biy-1906-22","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48020071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

A novel 1,4-naphthoquinone-derived compound induces apoptotic cell death in breast cancer cells 一种新型1,4-萘醌衍生化合物诱导乳腺癌细胞凋亡

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2019-08-05 DOI: 10.3906/biy-1901-19

Didem Karakas, R. Akar, Zeliha Gökmen, N. Deniz, E. Ulukaya

{"title":"A novel 1,4-naphthoquinone-derived compound induces apoptotic cell death in breast cancer cells","authors":"Didem Karakas, R. Akar, Zeliha Gökmen, N. Deniz, E. Ulukaya","doi":"10.3906/biy-1901-19","DOIUrl":"https://doi.org/10.3906/biy-1901-19","url":null,"abstract":"Breast cancer is the most-diagnosed cancer type among women. The triple-negative subtype is an especially aggressive type of breast cancer. Although chemotherapy is almost the only option for the treatment of triple-negative breast cancer (TNBC), currently used chemotherapeutics are not effective enough, considering the poor survival rate of patients. Therefore, novel compounds need to be developed to improve survival rates. It has been known that quinonic compounds, which are found in nature, have antibacterial, antifungal, and antitumorigenic properties. Naphthoquinones are members of the quinone family and are widely used in research due to their promising properties. In this study, we evaluated the cytotoxic activity of a novel naphthoquinone-derived compound (1,4-naphthoquinone (1,4-NQ)) against two different breast cancer cells: a hormone-responsive cell line (MCF-7) and a triple-negative cell line (MDA-MB-231). As a result, 1,4-NQ decreased cell viability in both tested cell lines in a dose-dependent manner. Increased apoptotic markers (presence of pyknotic nuclei, annexin-V positivity, caspase 3/7 activity, and decreased mitochondrial membrane potential) and DNA damage were especially observed in MDA-MB-231 cells after treatment with the compound. Considering the promising cytotoxic effect of the compound, 1,4-NQ needs further evaluation as a potential candidate for the treatment of TNBC.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2019-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/biy-1901-19","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45924891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Characterizing microsatellite polymorphisms using assembly-based and mapping-based tools 使用基于组装和映射的工具表征微卫星多态性

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2019-08-05 DOI: 10.3906/biy-1903-16

Gülfem Demi̇r, C. Alkan

{"title":"Characterizing microsatellite polymorphisms using assembly-based and mapping-based tools","authors":"Gülfem Demi̇r, C. Alkan","doi":"10.3906/biy-1903-16","DOIUrl":"https://doi.org/10.3906/biy-1903-16","url":null,"abstract":"Microsatellite polymorphism has always been a challenge for genome assembly and sequence alignment due to sequencing errors, short read lengths, and high incidence of polymerase slippage in microsatellite regions. Despite the information they carry being very valuable, microsatellite variations have not gained enough attention to be a routine step in genome sequence analysis pipelines. After the completion of the 1000 Genomes Project, which aimed to establish the most detailed genetic variation catalog for humans, the consortium released only two microsatellite prediction sets generated by two tools. Many other large research efforts have failed to shed light on microsatellite variations. We evaluated the performance of three different local assembly methods on three different experimental settings, focusing on genotype-based performance, coverage impact, and preprocessing including flanking regions. All these experiments supported our initial expectations on assembly. We also demonstrate that overlap-layout-consensus (OLC)-basedassembly methods show higher sensitivity to microsatellite variant calling when compared to a de Bruijn graph-based approach. We conclude that assembly with OLC is the better method for genotyping microsatellites. Our pipeline is available at https://github.com/gulfemd/STRAssembly.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2019-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/biy-1903-16","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47010862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Keratin 14 is a novel interaction partner of keratinocyte differentiation regulator: receptor-interacting protein kinase 4 角蛋白14是角化细胞分化调节剂受体相互作用蛋白激酶4的一个新的相互作用伙伴

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2019-08-05 DOI: 10.3906/BIY-1904-37

Ceren Sümer, Asiye Büşra Boz Er, Tuba Dinçer

{"title":"Keratin 14 is a novel interaction partner of keratinocyte differentiation regulator: receptor-interacting protein kinase 4","authors":"Ceren Sümer, Asiye Büşra Boz Er, Tuba Dinçer","doi":"10.3906/BIY-1904-37","DOIUrl":"https://doi.org/10.3906/BIY-1904-37","url":null,"abstract":"The epidermis, the outer layer of the skin, is formed by stratified keratinocyte layers. The self-renewal of the epidermis is provided by sustained proliferation and differentiation of the keratinocyte stem cells localized to the basal layer of the epidermis. Receptor-interacting protein kinase 4 (RIPK4) is an important regulator of keratinocyte differentiation, mutations of which are associated with congenital ectodermal malformations. In an attempt to identify the molecular basis of RIPK4’s function, we applied yeast two-hybrid screen (Y2H) and found basal layer-specific keratin filament component keratin 14 (KRT14) as a novel RIPK4-interacting partner. During keratinocyte differentiation, layer-specific keratin composition is tightly regulated. Likewise, the basal layer specific KRT14/keratin 5 (KRT5) heterodimers are replaced by keratin 1 (KRT1)/keratin 10 (KRT10) in suprabasal layers. The regulation of keratin turnover is under the control of signaling associated with posttranslational modifications in which phosphorylation plays a major role. In this study, we verified the KRT14-RIPK4 interaction, which was identified with Y2H, in mammalian cells and showed that the interaction was direct by using proteins expressed in bacteria. According to our results, the N-terminal kinase domain of RIPK4 is responsible for KRT14-RIPK4 interaction; however, the RIPK4 kinase activity is dispensable for the interaction. In accordance with their interaction, RIPK4 and KRT14 colocalize within the cells, particularly at keratin filaments associated with perinuclear ring-like structures. Moreover, RIPK4 did not show any effect on KRT14/KRT5 heterodimer formation. Our results suggest that RIPK4 may regulate the keratin turnover required for keratinocyte differentiation through interacting with KRT14.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2019-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1904-37","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49099696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Fibrous bone tissue engineering scaffolds prepared by wet spinning of PLGA PLGA湿法纺丝制备纤维骨组织工程支架

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2019-08-05 DOI: 10.3906/biy-1904-63

Nergis Abay Akar, Görke Gürel Peközer, G. Torun Köse

{"title":"Fibrous bone tissue engineering scaffolds prepared by wet spinning of PLGA","authors":"Nergis Abay Akar, Görke Gürel Peközer, G. Torun Köse","doi":"10.3906/biy-1904-63","DOIUrl":"https://doi.org/10.3906/biy-1904-63","url":null,"abstract":"Having a self-healing capacity, bone is very well known to regenerate itself without leaving a scar. However, critical size defects due to trauma, tumor, disease, or infection involve bone graft surgeries in which complication rate is relatively at high levels. Bone tissue engineering appears as an alternative for grafting. Fibrous scaffolds are useful in tissue engineering applications since they have a high surface-to-volume ratio, and adjustable, highly interconnected porosity to enhance cell adhesion, survival, migration, and proliferation. They can be produced in a wide variety of fiber sizes and organizations. Wet spinning is a convenient way to produce fibrous scaffolds with consistent fiber size and good mechanical properties. In this study, a fibrous bone tissue engineering scaffold was produced using poly(lactic-co-glycolic acid) (PLGA). Different concentrations (20%, 25%, and 30%) of PLGA (PLA:PGA 75:25) (Mw = 66,000-107,000) were wet spun using coagulation baths composed of different ratios (75:25, 60:40, 50:50) of isopropanol and distilled water. Scanning electron microscopy (SEM) and in vitro degradation studies were performed to characterize the fibrous PLGA scaffolds. Mesenchymal stem cells were isolated from rat bone marrow, characterized by flow cytometry and seeded onto scaffolds to determine the most appropriate fibrous structure for cell proliferation. According to the results of SEM, degradation studies and cell proliferation assay, 20% PLGA wet spun in 60:40 coagulation bath was selected as the most successful condition for the preparation of wet-spun scaffolds. Wet spinning of different concentrations of PLGA (20%, 25%, 30%) dissolved in dichloromethane using different isopropanol:distilled water ratios of coagulation baths (75:25, 60:40, 50:50) were shown in this study.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2019-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45536348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

A magnetic affinity approach to identify plant GABA-binding proteins 一种鉴定植物gaba结合蛋白的磁亲和方法

IF 2.2 4区生物学

Turkish Journal of Biology Pub Date : 2019-08-02 DOI: 10.3906/biy-1901-79

Jie Zou, Jingzhe Guo, Shisheng Li

引用次数: 1