一种鉴定植物gaba结合蛋白的磁亲和方法

IF 1.1 4区生物学 Q3 BIOLOGY

Turkish Journal of Biology Pub Date : 2019-08-02 DOI:10.3906/biy-1901-79

Jie Zou, Jingzhe Guo, Shisheng Li

{"title":"一种鉴定植物gaba结合蛋白的磁亲和方法","authors":"Jie Zou, Jingzhe Guo, Shisheng Li","doi":"10.3906/biy-1901-79","DOIUrl":null,"url":null,"abstract":"In plants, GABA plays a critical role in sexual plant reproduction; however, GABA receptors and the associated detailed signaling mechanisms remain to be elucidated. Our experiments show that the proposed technique is reliable and convenient for probing GABA-binding proteins and could be applicable in similar projects by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl). New probes produced by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl) can obtain useful information on GABA receptors in plants.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":"43 1","pages":"246 - 255"},"PeriodicalIF":1.1000,"publicationDate":"2019-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/biy-1901-79","citationCount":"1","resultStr":"{\"title\":\"A magnetic affinity approach to identify plant GABA-binding proteins\",\"authors\":\"Jie Zou, Jingzhe Guo, Shisheng Li\",\"doi\":\"10.3906/biy-1901-79\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In plants, GABA plays a critical role in sexual plant reproduction; however, GABA receptors and the associated detailed signaling mechanisms remain to be elucidated. Our experiments show that the proposed technique is reliable and convenient for probing GABA-binding proteins and could be applicable in similar projects by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl). New probes produced by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl) can obtain useful information on GABA receptors in plants.\",\"PeriodicalId\":23358,\"journal\":{\"name\":\"Turkish Journal of Biology\",\"volume\":\"43 1\",\"pages\":\"246 - 255\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2019-08-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.3906/biy-1901-79\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Turkish Journal of Biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.3906/biy-1901-79\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Turkish Journal of Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3906/biy-1901-79","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOLOGY","Score":null,"Total":0}

引用次数: 1

摘要

在植物中，GABA在植物有性繁殖中起着关键作用；然而，GABA受体及其相关的详细信号机制仍有待阐明。我们的实验表明，所提出的技术对于探测GABA结合蛋白是可靠和方便的，并且可以通过将GABA的游离羧基共价固定在磁珠（SiMAG羧基）上而应用于类似的项目。通过将GABA的游离羧基共价固定在磁珠（SiMAG羧基）上产生的新探针可以获得植物中GABA受体的有用信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

A magnetic affinity approach to identify plant GABA-binding proteins

In plants, GABA plays a critical role in sexual plant reproduction; however, GABA receptors and the associated detailed signaling mechanisms remain to be elucidated. Our experiments show that the proposed technique is reliable and convenient for probing GABA-binding proteins and could be applicable in similar projects by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl). New probes produced by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl) can obtain useful information on GABA receptors in plants.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Turkish Journal of Biology BIOLOGY-

CiteScore

4.60

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The Turkish Journal of Biology is published electronically 6 times a year by the Scientific and Technological Research Council of Turkey (TÜBİTAK) and accepts English-language manuscripts concerning all kinds of biological processes including biochemistry and biosynthesis, physiology and metabolism, molecular genetics, molecular biology, genomics, proteomics, molecular farming, biotechnology/genetic transformation, nanobiotechnology, bioinformatics and systems biology, cell and developmental biology, stem cell biology, and reproductive biology. Contribution is open to researchers of all nationalities.