一种鉴定植物gaba结合蛋白的磁亲和方法

IF 1.1 4区生物学 Q3 BIOLOGY

Turkish Journal of Biology Pub Date : 2019-08-02 DOI:10.3906/biy-1901-79

Jie Zou, Jingzhe Guo, Shisheng Li

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引用次数: 1

摘要

在植物中，GABA在植物有性繁殖中起着关键作用；然而，GABA受体及其相关的详细信号机制仍有待阐明。我们的实验表明，所提出的技术对于探测GABA结合蛋白是可靠和方便的，并且可以通过将GABA的游离羧基共价固定在磁珠（SiMAG羧基）上而应用于类似的项目。通过将GABA的游离羧基共价固定在磁珠（SiMAG羧基）上产生的新探针可以获得植物中GABA受体的有用信息。

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A magnetic affinity approach to identify plant GABA-binding proteins

In plants, GABA plays a critical role in sexual plant reproduction; however, GABA receptors and the associated detailed signaling mechanisms remain to be elucidated. Our experiments show that the proposed technique is reliable and convenient for probing GABA-binding proteins and could be applicable in similar projects by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl). New probes produced by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl) can obtain useful information on GABA receptors in plants.

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来源期刊

Turkish Journal of Biology BIOLOGY-

CiteScore

4.60

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The Turkish Journal of Biology is published electronically 6 times a year by the Scientific and Technological Research Council of Turkey (TÜBİTAK) and accepts English-language manuscripts concerning all kinds of biological processes including biochemistry and biosynthesis, physiology and metabolism, molecular genetics, molecular biology, genomics, proteomics, molecular farming, biotechnology/genetic transformation, nanobiotechnology, bioinformatics and systems biology, cell and developmental biology, stem cell biology, and reproductive biology. Contribution is open to researchers of all nationalities.