Muhammad Ali, Shahid Y Khan, Hira Butt, Ryan C Navarro, Jessica M Izzi, S Amer Riazuddin
{"title":"Pluripotent Stem Cell-Derived Corneal Endothelial Cells Form a Functional Corneal Endothelium in Canines.","authors":"Muhammad Ali, Shahid Y Khan, Hira Butt, Ryan C Navarro, Jessica M Izzi, S Amer Riazuddin","doi":"10.1167/tvst.14.10.5","DOIUrl":"10.1167/tvst.14.10.5","url":null,"abstract":"<p><strong>Purpose: </strong>We previously reported that cryopreserved human embryonic stem cell (hESC)-derived corneal endothelial cells (CECs) can form a functional corneal endothelium (CE) on denuded Descemet's membrane (DM) in rabbits and monkeys. Here, we extend the utility of cryopreserved CECs to develop a possible treatment for canines with corneal endothelial dysfunction.</p><p><strong>Methods: </strong>We developed a corneal endothelial dysfunction canine model (n = 2) by mechanical scraping of central (8 mm diameter) CE. To regenerate the CE, the canines received an injection of cryopreserved hESC-derived CECs in the anterior chamber of the eye, followed by being placed in a cornea-down position to allow the injected cells to settle on denuded DM. The structural integrity, pachymetry, CE cell density and morphology of the regenerated and the CE of the untreated eyes were examined by optical coherence tomography (OCT), and a specular microscope. Immunohistochemical (IHC) analysis of the regenerated and resident CE was completed with a human-specific nucleoli antibody and other antibodies.</p><p><strong>Results: </strong>The corneas of the cryopreserved hESC-derived CEC-injected eyes developed corneal edema within 24 hours after scraping of the CE and cell injection but regained transparency in one to two weeks after the CEC injection. OCT revealed anatomically normal corneas with pachymetry of the CEC-injected eyes similar to the untreated left eyes, and specular microscopy examination illustrated regenerated CE exhibiting hexagonal/polygonal cells with CEC density of the regenerated CE comparable to the CE of the untreated eye. IHC analysis confirmed the regeneration of a functional CE resulting from the settlement of injected cryopreserved hESC-derived CECs on the denuded DM.</p><p><strong>Conclusions: </strong>The results confirm the regeneration of a functional CE in canines resulting from the settlement of cryopreserved hESC-derived CECs on the denuded DM.</p><p><strong>Translational relevance: </strong>Our data confirm the efficacy of cryopreserved pluripotent stem cell-derived CECs as a therapeutic option to regenerate a functional CE in canines with corneal endothelial dysfunction.</p>","PeriodicalId":23322,"journal":{"name":"Translational Vision Science & Technology","volume":"14 10","pages":"5"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12510385/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Linyu Ni, Alexus Warchock, Erik Krawczyk, Wonsuk Kim, Sayoko E Moroi, Guan Xu, Alan Argento
{"title":"Biomechanical Relationships Between the Aqueous Veins and Perilimbal Sclera During Intraocular Pressure Regulation.","authors":"Linyu Ni, Alexus Warchock, Erik Krawczyk, Wonsuk Kim, Sayoko E Moroi, Guan Xu, Alan Argento","doi":"10.1167/tvst.14.10.9","DOIUrl":"https://doi.org/10.1167/tvst.14.10.9","url":null,"abstract":"<p><strong>Purpose: </strong>A major assumption of microinvasive glaucoma surgeries (MIGS) is that effective intraocular pressure (IOP) reduction can be achieved by enhancing aqueous humor (AH) outflow into the aqueous veins. However, this IOP outcome is not predictable in part due to lack of knowledge concerning the role of tissue biomechanics on AH outflow through the distal aqueous veins within the perilimbal sclera (PLS). The purpose of this study is to investigate the biomechanical response of the aqueous veins and the PLS and their interaction during IOP.</p><p><strong>Methods: </strong>Optical resolution photoacoustic microscopy were implemented to capture the 3D deformations of the aqueous veins and in the surrounding PLS during aqueous outflow in porcine and human whole globes. Partial trabeculotomy was performed to minimize the contribution of the trabecular meshwork to the outflow resistance.</p><p><strong>Results: </strong>The deformations of the tissue components and the IOP were measured in both porcine and human eyes. Strong correlations were found among the relative cross-sectional area of the aqueous veins, strains in the PLS in porcine eyes, and the steady-state (SS) IOP of porcine eyes.</p><p><strong>Conclusions: </strong>The data shows that stiff PLS restricts vein cross-sectional area and results in overall higher SS IOP in an eye.</p><p><strong>Translational relevance: </strong>The findings in this study suggest the potential for predicting the limit of effectiveness of an MIGS given knowledge of the interactive behaviors of the aqueous veins and PLS in an eye.</p>","PeriodicalId":23322,"journal":{"name":"Translational Vision Science & Technology","volume":"14 10","pages":"9"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145252827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiao Tian, Yu Liu, Xiaoling Ning, Qin Ma, Kang Hou, Jinhan Yao, Zhao Wang, Xiaogang Wang
{"title":"3D-OCTA Evaluation of Retinal Microcirculation Abnormalities in the Macular Region of Eyes With Anisometropic Amblyopia.","authors":"Xiao Tian, Yu Liu, Xiaoling Ning, Qin Ma, Kang Hou, Jinhan Yao, Zhao Wang, Xiaogang Wang","doi":"10.1167/tvst.14.10.4","DOIUrl":"10.1167/tvst.14.10.4","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate retinal microvascular functional differences among amblyopic eyes, fellow eyes of patients with anisometropic amblyopia, and healthy controls using three-dimensional optical coherence tomography angiography (3D-OCTA).</p><p><strong>Methods: </strong>This study included 52 participants (78 eyes): 26 amblyopic eyes, 26 fellow eyes in anisometropic amblyopia patients, and 26 age-matched healthy controls. Using 3D-OCTA, retinal microvascular parameters in the parafoveal region were evaluated, including vessel tortuosity index (VTI), vessel volume density (VVD), vessel skeleton density (VSD), and vessel diameter index (VDI) in the superficial vascular plexus, intermediate capillary plexus, and deep capillary plexus. Intergroup differences were analyzed using generalized estimating equations with Bonferroni-adjusted pairwise comparisons.</p><p><strong>Results: </strong>Both amblyopic and fellow eyes in anisometropic amblyopia patients showed reduced VVD and VSD across multiple sectors compared to healthy controls, with further reductions in amblyopic eyes in select regions. The VDI was elevated in both amblyopic and fellow eyes relative to healthy controls (P < 0.05), with no difference between the two (P = 1.00). The VTI was significantly higher in amblyopic eyes than in healthy controls (1.42 ± 0.005 vs. 1.41 ± 0.019; P = 0.01), but differences involving fellow eyes were not significant (P > 0.3).</p><p><strong>Conclusions: </strong>Amblyopic eyes showed decreased retinal perfusion, with increased vessel diameter and tortuosity, and fellow eyes in anisometropic amblyopia patients also demonstrated deviations in vascular parameters compared to healthy controls.</p><p><strong>Translational relevance: </strong>Retinal vascular changes detected by 3D-OCTA may provide non-invasive biomarkers for early detection, progression monitoring, and treatment evaluation of anisometropic amblyopia.</p>","PeriodicalId":23322,"journal":{"name":"Translational Vision Science & Technology","volume":"14 10","pages":"4"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12514982/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Blood-Based Cell-Free DNA in Ophthalmic Oncology.","authors":"Jasmine H Francis, David H Abramson","doi":"10.1167/tvst.14.10.7","DOIUrl":"10.1167/tvst.14.10.7","url":null,"abstract":"<p><strong>Purpose: </strong>Cell-free DNA (cfDNA) has emerged as a useful liquid biopsy biomarker with many translational applications in oncology. Here, we review the uses of blood-based cfDNA in ophthalmic oncology, with a focus on uveal melanoma, retinoblastoma, conjunctival tumors, choroidal metastases, indeterminate choroidal lesions, and ocular histiocytosis.</p><p><strong>Methods: </strong>A systematic literature search in MEDLINE, PubMed, Web of Science, and Scopus from October 2017 to June 2025 was performed. Data extraction included tumor diagnosis, number of patients in the cohort, characteristics of the blood-based cfDNA assay employed (including technology used for mutation identification), number and types of genes analyzed, performance metrics for the assay, and any clinical impact.</p><p><strong>Results: </strong>The use of blood-based cfDNA in the field of ophthalmic oncology has been studied to varying degrees. Perhaps its most well-researched area is uveal melanoma, in which applications for both primary and metastatic uveal melanoma continue to be evaluated. Its use in retinoblastoma has been investigated by a small number of groups. There is minimal but intriguing work on the use of blood-based cfDNA in for conjunctival tumors, choroidal metastases, tumors of indeterminate origin, and ocular histiocytosis.</p><p><strong>Conclusions: </strong>cfDNA science bridges the gap between basic research and clinical care. Within the field of ophthalmic oncology, cfDNA may be useful as a diagnostic/prognostic tool, for surveillance of metastatic or minimally residual disease, to identify driver mutations for treatment selection, as a biomarker of treatment response, for screening for recurrent or new cancer, and to detect new molecule clones.</p>","PeriodicalId":23322,"journal":{"name":"Translational Vision Science & Technology","volume":"14 10","pages":"7"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12514988/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145233533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anat Galor, Bonnie Henderson, Cristos Ifantides, James A Stefater, Tomasz P Stryjewski
{"title":"The Safety and Tolerability of a Single Scleral Treatment With a Cooling Device in Rabbits.","authors":"Anat Galor, Bonnie Henderson, Cristos Ifantides, James A Stefater, Tomasz P Stryjewski","doi":"10.1167/tvst.14.10.2","DOIUrl":"10.1167/tvst.14.10.2","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the preclinical safety of a single treatment of a topical scleral cooling device.</p><p><strong>Methods: </strong>Two Good Laboratory Practice prospective preclinical device safety studies were performed: study 1 in normal healthy rabbit eyes (n = 5) and study 2 in healthy rabbit eyes that underwent a surgically induced corneal epithelial defect (n = 3). Following sedation, eyes were treated for 10 minutes by applying the cooling device to the sclera. Key assessments for both studies included clinical observations, Schirmer's I and II tests, ophthalmic examinations, intraocular pressure (IOP) measurements, and histopathology. Study 1 assessments were performed on days -3, 7, 14, 21, 28, 42 (±2 days), and 56 (±2 days). In study 2, a 3-mm diameter corneal defect was created in both eyes of each animal 14 days after the cooling device application. Corneal fluorescein staining was monitored at 3, 6, 24, 30, 48, 54, and 72 hours after defect creation.</p><p><strong>Results: </strong>Minimal changes in conjunctival redness, peripheral corneal haze, and fluorescein staining were observed, which completely self-resolved within 4 weeks after treatment. Schirmer's I and II and IOP results were within normal ranges in all animals. All epithelial defects in both groups had fully healed within 48 hours.</p><p><strong>Conclusions: </strong>No adverse effects were observed within 8 weeks following a single scleral application of the topical cooling device in rabbit eyes.</p><p><strong>Translational relevance: </strong>This article introduces a novel ocular cooling device for the treatment of chronic ocular surface pain, a condition with a considerable unmet need for more effective therapies.</p>","PeriodicalId":23322,"journal":{"name":"Translational Vision Science & Technology","volume":"14 10","pages":"2"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12510379/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145201173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jenny Park, Mohammad Kazemi, Mitalee Tamhane, Jie Shen
{"title":"Computational Fluid Dynamics Modeling of Intravitreal Ranibizumab Bolus Versus Subretinal ABBV-RGX-314 Transgene Product in Human Eyes.","authors":"Jenny Park, Mohammad Kazemi, Mitalee Tamhane, Jie Shen","doi":"10.1167/tvst.14.10.6","DOIUrl":"10.1167/tvst.14.10.6","url":null,"abstract":"<p><strong>Purpose: </strong>ABBV-RGX-314 is being developed for neovascular age-related macular degeneration (nAMD). Computational fluid dynamics (CFDs) modeling in the eye enables simulation of drug distribution incorporating geometry and substructures of the eye across species. Given the similarity between ranibizumab and ABBV-RGX-314 transgene product (TP), ranibizumab intraocular pharmacokinetic (PK) data from literature were used to simulate intraocular drug distribution of ABBV-RGX-314 TP. This investigation aims to use CFD modeling to estimate retinal TP level based on aqueous humor (AH) TP level following subretinal (SR) injection of ABBV-RGX-314 in patients with nAMD.</p><p><strong>Methods: </strong>Ocular distribution of ranibizumab following a single intravitreal (IVT) injection was modeled in both monkey and human eyes independently. Following model validation, ABBV-RGX-314 TP distribution in human eyes was simulated following retinal transduction of ABBV-RGX-314.</p><p><strong>Results: </strong>Iterative simulations were performed to achieve similar AH ABBV-RGX-314 TP levels in patients with nAMD from phase I/IIa Study RGX-314-001. The CFD simulation estimated corresponding retinal TP concentrations of 1.86 to 5.50 µg/g at steady-state, which was assumed to be reached by 28 days and falls within the range of the estimated retinal ranibizumab trough retinal ranibizumab concentration (Ctrough; 0.718-5.37 µg/g) following monthly and every other month (EOM) dosing of 0.5 mg ranibizumab in patients with nAMD.</p><p><strong>Conclusions: </strong>The current study results predict that the 2 pivotal trial ABBV-RGX-314 doses (6.4E10 and 1.3E11 genome copies/eye) are expected to achieve and maintain sufficient retinal ABBV-RGX-314 TP levels for the treatment of nAMD.</p><p><strong>Translational relevance: </strong>CFD modeling effectively bridges limited human ocular PK data with rich preclinical data, supporting model-informed drug development (MIDD) for clinical dose selection.</p>","PeriodicalId":23322,"journal":{"name":"Translational Vision Science & Technology","volume":"14 10","pages":"6"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12510396/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Francesco Martino, Sonia Ortiz-Peregrina, Miriam Casares-López, Pilar Granados-Delgado, José J Castro-Torres, Rosario G Anera
{"title":"Influence of Cannabidiol Use on Visual Function and Optical Quality: A Randomized Controlled Trial.","authors":"Francesco Martino, Sonia Ortiz-Peregrina, Miriam Casares-López, Pilar Granados-Delgado, José J Castro-Torres, Rosario G Anera","doi":"10.1167/tvst.14.10.10","DOIUrl":"https://doi.org/10.1167/tvst.14.10.10","url":null,"abstract":"<p><strong>Purpose: </strong>To study the influence of different concentrations of vaporized cannabidiol (CBD; 0%, 15%, and 30%) on visual function and optical quality.</p><p><strong>Methods: </strong>A randomized, crossover, double-blind, placebo-controlled, experimental study was conducted. A total of 30 participants with a mean age of 26.0 ± 6.3 years completed the study. Placebo (0 mg of CBD), 15% (16 mg of CBD), and 30% (32 mg of CBD) concentrations of CBD were employed. Visual function was evaluated through various tests, including pupil size, static and dynamic visual acuity, contrast sensitivity, dot motion detection, visual disturbance index (VDI), and stereoacuity. Optical quality was assessed by log(s), modulation transfer function (MTF) cutoff, objective scattering index (OSI), and Strehl ratio. Self-perceived visual effects were also recorded.</p><p><strong>Results: </strong>There was no significant impairment of static and dynamic visual acuity. Contrast sensitivity was unaffected by CBD use. Mean dot motion detection showed no differences among the three concentrations. In optical quality, none of the parameters worsened under CBD use. No changes were observed for the VDI. In addition, no deterioration was observed for stereoacuity at distance or near. No changes in pupil size were found after CBD consumption.</p><p><strong>Conclusions: </strong>This non-psychotropic CBD did not appear to adversely affect vision and seems to be a safe substance in the short term at the concentrations assessed.</p><p><strong>Translational relevance: </strong>The use of this cannabinoid would not be dangerous for tasks that rely heavily on vision. This study could be useful and helpful for evidence-based decision-making for public health policy on its use.</p>","PeriodicalId":23322,"journal":{"name":"Translational Vision Science & Technology","volume":"14 10","pages":"10"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145275913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparisons of Visibility of Two-Color (RG) to Three-Color (RGB) Ultra-Widefield Images for Ten Types of Fundus Lesions.","authors":"Keitaro Mizumoto, Kumiko Kato, Hisashi Matsubara, Yoshitsugu Matsui, Shinichiro Chujo, Yoko Mase, Yukiko Muramoto, Kengo Ikesugi, Mineo Kondo","doi":"10.1167/tvst.14.10.11","DOIUrl":"https://doi.org/10.1167/tvst.14.10.11","url":null,"abstract":"<p><strong>Purpose: </strong>To compare the visibility of fundus lesions between RGB and RG images obtained with an ultra-widefield (UWF) fundus imaging device (Optos) for 10 types of fundus lesions.</p><p><strong>Methods: </strong>UWF images from 30 patients representing 10 types of fundus lesions were analyzed: vessel sheathing, optic disc cupping, cotton wool spots, epiretinal membrane, laser photocoagulation scars, retinal drusen, retinal hemorrhage, retinal/choroidal detachment, chorioretinal atrophy, and macular degeneration. Three images of each type of lesion were used, and 26 board-certified ophthalmologists compared them. The raters compared the visibility of lesions on a five-point scale: RG significantly better = -2; RG slightly better = -1; equal = 0; RGB slightly better = +1; and RGB significantly better = +2. The Wilcoxon signed-rank test was used to determine the significance of the differences.</p><p><strong>Results: </strong>RGB images were rated significantly more visually favorable than the RG images for all 10 lesions (P < 0.01). The greatest improvements in perceived visibility in RGB images were observed for vessel sheathing (50.7%), optic disc cupping (49.8%), cotton wool spots (46.9%), and an epiretinal membrane (46.7%). Conversely, macular degeneration (22.7%) and chorioretinal atrophy (25.1%) had minimal advantages in RGB images.</p><p><strong>Conclusions: </strong>RGB imaging improves the visibility of white and superficial fundus lesions but adds little benefit for deeper located lesions.</p><p><strong>Translational relevance: </strong>The results indicate that RGB imaging, which includes blue laser light, improves the visibility of superficial and white retinal lesions. These findings support the optimized use of color imaging modalities in clinical practice based on lesion characteristics.</p>","PeriodicalId":23322,"journal":{"name":"Translational Vision Science & Technology","volume":"14 10","pages":"11"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145275941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Karen M Chen, Kevin W Chen, Vlad Diaconita, Stanley Chang, Leejee H Suh
{"title":"OphthoACR (Ophthalmology Automated Chart Review): An AI-Powered Tool for Complete Automation of Ophthalmology Chart Reviews and Cohort Data Analysis.","authors":"Karen M Chen, Kevin W Chen, Vlad Diaconita, Stanley Chang, Leejee H Suh","doi":"10.1167/tvst.14.10.8","DOIUrl":"https://doi.org/10.1167/tvst.14.10.8","url":null,"abstract":"<p><strong>Purpose: </strong>Retrospective chart reviews in ophthalmology are essential for gaining clinical insights, but they remain labor-intensive and prone to error. Despite digitization through electronic health records, extracting and interpreting lengthy, unstructured patient histories remains challenging, particularly in ophthalmology, which relies heavily on both imaging and text-based reports. We introduce OphthoACR, a Health Insurance Portability and Accountability Act-compliant artificial intelligence (AI)-powered tool for automated chart review and cohort analyses in ophthalmology.</p><p><strong>Methods: </strong>OphthoACR was applied to extract 16 variables of increasing task difficulty from the complete chart histories of 91 patients who underwent secondary intraocular lens surgery at the Columbia University Irving Medical Center from January 2020 to August 2024, for a total of 5834 unique documents. The tool integrates a fine-tuned large language model into a robust pipeline to extract and contextualize unstructured clinical data, including operative reports and imaging documents. OphthoACR's performance was compared to manual and AI-assisted chart reviews.</p><p><strong>Results: </strong>OphthoACR achieved 94% accuracy in extracting variables of interest, significantly outperforming manual review (83%). It demonstrated 97% specificity, 92% sensitivity, and a Cohen's κ of 0.70, indicating robust agreement. Average time for OphthoACR to process a patient chart was 80 seconds, a 95% reduction compared to the manual review's average of 25.2 minutes. For cohort-wide processing, the improvement was 99.9% due to parallel processing of patients' charts.</p><p><strong>Conclusions: </strong>OphthoACR significantly improves the accuracy and efficiency of ophthalmology chart reviews, offering an unprecedented automated solution to analyze large patient cohorts.</p><p><strong>Translational relevance: </strong>OphthoACR provides end-to-end automation of retrospective chart reviews, transforming the currently labor-intensive manual process into an efficient, accurate, and scalable solution that substantially enhances clinical research.</p>","PeriodicalId":23322,"journal":{"name":"Translational Vision Science & Technology","volume":"14 10","pages":"8"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145252822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Early Fluctuations of Exudative Lesions as Predictors of Two-Year Clinical Outcomes in Neovascular Age-Related Macular Degeneration.","authors":"Jiaxin Pu, Xuenan Zhuang, Miaoling Li, Xinlei Hao, Guiqin He, Yongyue Su, Linling Xia, Feng Wen","doi":"10.1167/tvst.14.10.1","DOIUrl":"10.1167/tvst.14.10.1","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate how early fluctuations of exudative lesions correlate with 2-year outcomes in neovascular age-related macular degeneration (nAMD).</p><p><strong>Methods: </strong>This prospective study included 92 treatment-naïve nAMD eyes treated with anti-vascular endothelial growth factor (anti-VEGF) therapy using a 3+PRN regimen over 2 years. Volumes of seven exudative lesion types were measured: subretinal fluid (SRF); intraretinal fluid (IRF); vascular and avascular subretinal hyperreflective material (vSHRM and avSHRM, respectively); and serous, fibrovascular, and hemorrhagic pigment epithelial detachment (sPED, fvPED, and hPED, respectively). Four fluctuation parameters were calculated during the 3-month loading phase. Relationships between these early fluctuations and clinical outcomes, including best-corrected visual acuity (BCVA) and injection frequency, were analyzed.</p><p><strong>Results: </strong>A total of 92 eyes (92 patients) were included, with a mean age of 65.65 ± 7.20 years. After adjusting for baseline characteristics, fluctuations in both vSHRM and avSHRM were identified as independent predictors of 2-year BCVA prognosis, with greater fluctuations associated with poorer visual outcomes (vSHRM Stdβ range, 0.671-0.797; avSHRM Stdβ range, 0.722-0.856; all P ≤ 0.001). However, only IRF fluctuation metrics were found to be independently associated with injection frequency over 2 years (Stdβ range, 0.327-0.532; all P < 0.05), with greater fluctuations correlating with more frequent injections.</p><p><strong>Conclusions: </strong>SHRM fluctuations during the loading phase predict 2-year visual outcomes, and IRF variations correlate with injection frequency in nAMD.</p><p><strong>Translational relevance: </strong>Early fluctuation patterns of exudative lesions can serve as predictive biomarkers to guide personalized anti-VEGF treatment strategies and optimize long-term visual outcomes in nAMD patients.</p>","PeriodicalId":23322,"journal":{"name":"Translational Vision Science & Technology","volume":"14 10","pages":"1"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12510388/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145200843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}