Transfusion Medicine最新文献

{"title":"Zika virus surveillance post-epidemic in blood donors from São Paulo, Brazil 2016-2020.","authors":"Suzete Cleusa Ferreira, José Eduardo Levi, Anna Shoko Nishiya, Cesar de Almeida-Neto, Jerenice Esdras Ferreira, Juliana Derriga, Nanci A Salles, Silvia Petrossi Gallo Polato, Katia C Dantas, Edmir Boturão-Neto, Martha Mathias Rocha, Vanderson Rocha, Alfredo Mendrone-Jr","doi":"10.1111/tme.70030","DOIUrl":"10.1111/tme.70030","url":null,"abstract":"<p><strong>Introduction: </strong>Zika virus (ZIKV) is primarily transmitted through the bite of the Aedes aegypti mosquito, though transmission via blood transfusion has also been documented. During the 2015 ZIKV epidemic in Brazil, severe complications were observed in pregnant women, leading to fetal microcephaly. This study evaluated the persistence of ZIKV in blood donated by healthy individuals during the post-epidemic period from 2016 to 2020.</p><p><strong>Methods: </strong>Blood donor samples from 109 296 individuals were screened for ZIKV RNA using nucleic acids extracted from plasma pools (six donors per pool). These samples had previously undergone routine nucleic acid testing (NAT) for HBV, HCV and HIV.</p><p><strong>Results: </strong>Viral RNA (Ribonucleic Acid) was detected in a donor sample from the city of Santos in May 2016, resulting in a prevalence of 0.0009%. The positive donor was confirmed through viral sequencing using the Sanger method. Sequencing and phylogenetic analysis of an envelope gene amplicon revealed that the Zika virus RNA detected belonged to the Asian clade. This Asian lineage strain emerged in Brazil, Fortaleza, in 2015, isolated in northeastern Brazil in 2015, an area where most cases of microcephaly associated with ZIKV have been reported. A follow-up sample collected 1 month after donation showed seroconversion.</p><p><strong>Conclusion: </strong>The detection of ZIKV RNA by NAT in a donated blood sample demonstrates that, although extremely rare, the virus is still present. Periodic active surveillance of blood donations for viruses associated with past outbreaks may help identify an incipient resurgence before it develops into a new epidemic.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":"59-65"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12882743/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145309274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of a new solid-phase ABO and RhD blood grouping kit.","authors":"Yuelong Xu, Rong Wang, Dongmei Ge, Xingyu Huang, Yuanming Yang, Zifan Meng, Haiyan Wang, Ying Li","doi":"10.1111/tme.70032","DOIUrl":"10.1111/tme.70032","url":null,"abstract":"<p><strong>Objectives: </strong>Rapid and accurate identification of blood groups is the foundation of emergency blood support. We performed a complete assessment of a new solid-phase kit for ABO forward grouping and RhD grouping (ABD Kit, InTec Products, Xiamen, China) on the analytical performance, which was compared with those of traditional methods.</p><p><strong>Methods: </strong>We analysed 1260 clinical samples using the ABD Kit, including weakly agglutinated samples of A, B and D antigen, and compared the test results with those via Gel card, test tube and slide methods. We also validated the results of typing blood samples containing weak antigens using first-generation gene sequencing.</p><p><strong>Results: </strong>The ABO forward group and RhD group of 1260 samples was determined using ABD Kit, revealing that the inter-batch repeatability rate of the kit was 100%. The results of the kit were compared with those of the gel card method, revealing that the detection accuracy of the kit was also 100%, which was confirmed by comparing the detection of weak antigen samples with the results of first-generation gene sequencing. The accuracy of the test tube agglutination method was 100%. In contrast, the accuracy of the slide agglutination method was low, especially in the detection of weak A blood antigens (agglutination strength 1+), weak B blood antigens (agglutination strength 1+) and weak D blood antigens (agglutination strength 1+ or 2+).</p><p><strong>Conclusions: </strong>The ABD Kit (InTec Products, Xiamen, China) showed high sensitivity, reproducibility and specificity, indicative of excellent analytical performance. It is a reliable, practical and promising solution for rapid and accurate identification of blood types.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":"28-36"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145281034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracorporeal photopheresis-New insights into an old procedure.","authors":"Sabine Seiffert, Janine Kirchberg, Enrica Bach, Victoria Menger, Mandy Brückner, Ulrich Sack, Ulrike Köhl, Uwe Platzbecker, Andreas Boldt, Marco Herling, Vladan Vučinić","doi":"10.1111/tme.13156","DOIUrl":"10.1111/tme.13156","url":null,"abstract":"<p><strong>Background: </strong>Extracorporeal photopheresis (ECP) is a safe immunomodulatory strategy that induces cell-type selective apoptosis through photodynamic processes. Despite decades of use, the mechanisms underlying ECP remain largely unexplored, particularly in studies examining specific immune cell subsets in ex vivo setups.</p><p><strong>Aims: </strong>This proof-of-concept pilot study presents data on apoptosis and proliferation of T-lymphocytes following ex vivo ECP application to leukocyte concentrates (LC) and peripheral blood (PB) samples from healthy donors.</p><p><strong>Methods: </strong>LC and PB were diluted to a haematocrit of 2% and treated with 8-methoxypsoralen, followed by ECP (ECP+) or no ECP (ECP-) in a discontinued system. Apoptosis of mononuclear cells was assessed 48 h post-ECP using annexin V and 7 Aminoactinomycin D (7-AAD) staining with flow-cytometric quantification. The proliferative capacity of non-apoptotic T-lymphocytes was measured after 72 h of post-ECP stimulation with anti-CD3/CD28 cross-linking, using Violet Proliferation Dye 450.</p><p><strong>Results: </strong>ECP exposure significantly reduced the median T-cell receptor-induced proliferation of viable T-lymphocytes from both LC (4.6%, p = 0.02) and PB (4.2%, p = 0.03). However, 7-AAD staining 48 h post-ECP showed no significant differences in the proportions of apoptotic cells in this experimental model.</p><p><strong>Conclusion: </strong>Ex vivo ECP treatment inhibited T-lymphocyte proliferation in both LC and PB from healthy individuals, suggesting this as a key mode of action. Our findings highlight ECP's potential applications, including its implications for modern immune therapies' adverse effects. Further analyses of functional characteristics of remaining vital cells are necessary.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":"53-58"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12882749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Absence of dengue, zika and chikungunya viral nucleic acid in blood donations in China.","authors":"Ru Xu, Ying Yan, Hui Han, Weichao Sun, Jianfeng Liang, Bin Li, Yuwei Zhao, Pincan Su, Yongshui Fu, Xia Rong, Lunan Wang","doi":"10.1111/tme.70027","DOIUrl":"10.1111/tme.70027","url":null,"abstract":"","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":"87-89"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145446018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Postpartum IV iron sucrappenose, and rates of RBC transfusion: A quality improvement initiative.","authors":"Itamar D Futterman, Erum Azhar, Nicole F Geller, Janet L Stein, Howard Minkoff","doi":"10.1111/tme.70028","DOIUrl":"10.1111/tme.70028","url":null,"abstract":"<p><strong>Background: </strong>Transfusions of packed red blood cells (RBC) have long been used to correct postpartum anaemia. Given the ongoing shortage of packed RBC and transfusion reactions, we sought to determine if the use of IV iron sucrose infusions could decrease transfusions in the postpartum period.</p><p><strong>Methods: </strong>We conducted a six-year quasi-experimental interrupted time series study from January 2016 to December 2021. The study was divided into three phases: 1. pre-intervention phase, 2. roll-out phase, and 3. post-intervention phase. Our intervention focused on a holistic transfusion prevention bundle that incorporated provider education regarding transfusion practices, antepartum optimization, and cell saver use. The main outcome was change over time from the use of IV iron sucrose over allogenic blood products in the setting of postpartum anaemia. Statistical process control (SPC) was used to explore the effect of our bundled intervention over time.</p><p><strong>Results: </strong>46 478 deliveries were recorded. The XmR control chart showed a shift between phases 1 and 3, as indicated with a special cause signal. The U control chart demonstrated that IV iron infusions increased from 5.6 per 1000 births in phase 1 to 136.4 per 1000 births in phase 3. On Poisson regression, the rate of IV iron sucrose infusions increased 19-fold [RI 19.65 (95% CI 16.00-24.14 p < 0.0001)], while the rate of transfusions decreased by 60% [RI 0.4 (95% CI 0.33-0.49 p < 0.001)] from pre- to post-intervention.</p><p><strong>Conclusion: </strong>A holistic transfusion prevention bundle was associated with both a reduction in the number of patients transfused and the rate of multiple-unit RBC transfusions.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":"37-44"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145309322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel missense variant of the RHAG gene in the first Iraqi case of the Rh-null phenotype.","authors":"Muqdad M N Al-Mousawi, Saad S Merza, Rozhgar A Khailany, Ali Abdulghani Ramadhan, Adil A Othman, Sanea A Ahmed, Nasir Al-Allawi","doi":"10.1111/tme.70029","DOIUrl":"10.1111/tme.70029","url":null,"abstract":"","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":"84-86"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145207623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tocilizumab provides a potential therapeutic option for the management of hyperhaemolysis syndrome in sickle cell disease: A case series and brief narrative overview of the literature.","authors":"S Wolf, B Singh, A Zaidi, P Greaves, F Oyesanya, S Bennett, B Kaya, F Barroso, P Telfer","doi":"10.1111/tme.70026","DOIUrl":"10.1111/tme.70026","url":null,"abstract":"<p><strong>Background and objectives: </strong>Hyperhaemolysis syndrome is a life-threatening complication of transfusion, potentially triggered by macrophage activation, with limited treatment options. Tocilizumab, an anti-IL6 monoclonal antibody, has mechanistic rationale for use and has been shown to be effective in a small number of cases. In this paper, we review four cases of hyperhaemolysis treated with tocilizumab in the context of the existing literature.</p><p><strong>Materials and methods: </strong>Cases of use of tocilizumab in hyperhaemolysis were identified from two large specialist haemoglobinopathy centres between the period January 2021 and March 2025. Clinical and laboratory data were collected.</p><p><strong>Results: </strong>Four cases of hyperhaemolysis treated with IVIG, steroids and tocilizumab were reported. In all cases, haemolysis responded rapidly to tocilizumab therapy. Two patients subsequently received RBC transfusions without haemolysis; two patients died from causes unrelated to haemolysis.</p><p><strong>Conclusions: </strong>This case series supports the use of tocilizumab as a therapeutic option for rapid resolution of haemolysis. It is generally widely available and should be considered a suitable and cost-effective alternative to currently available options.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":"66-71"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12882761/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145259207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening, genetic analysis, and clinical transfusion implications of thalassaemia and glucose-6-phosphate dehydrogenase deficiency in blood donors.","authors":"Zhi-Xiao Chen, Bao-Ying Chen, Rong-Huo Liu, Jian-Cheng Huang, Jia-Min Mo, Zhen-Yuan Mai, Yan-Qing Zeng, Yu-Chan Huang, Yan-Bin Cao, Bai-Ru Lai, Wei-Feng Xu, Li-Ye Yang","doi":"10.1111/tme.70018","DOIUrl":"10.1111/tme.70018","url":null,"abstract":"<p><strong>Background: </strong>This study aims to explore the prevalence, genotypes, and clinical applications of thalassaemia and glucose-6-phosphate dehydrogenase (G6PD) deficiency among blood donors, offering scientific support for the safety of clinical blood transfusions.</p><p><strong>Materials and methods: </strong>Haematological and G6PD enzyme activity tests were conducted on 1028 blood donors from one coastal city in southern China. A detailed genotypic analysis was performed on samples potentially carrying thalassaemia and G6PD deficiency, and their clinical significance was subsequently explored.</p><p><strong>Results: </strong>Among 1028 blood donors, 137 individuals were found to carry the thalassaemia gene (13.33%). The specific distribution is as follows: 111 cases of α-thalassaemia (10.80%), 21 cases of β-thalassaemia (2.04%), and five cases with both α- and β-thalassaemia (0.49%). The -^SEA/αα genotype was the most common thalassaemia genotype (8.17%). Additionally, 121 individuals (11.77%) carried the G6PD deficiency gene, with the c.1388G>A variant being the most prevalent (3.7%). Compared to recipients of blood from normal donors, patients who received transfusions from donors with thalassaemia or G6PD deficiency did not exhibit significant differences in the risk of adverse reactions or the extent of haemoglobin concentration elevation.</p><p><strong>Conclusion: </strong>The -^SEA/αα genotype is the most prevalent in α-thalassaemia, while c.1388G>A is the most common genetic variant in G6PD deficiency. Individuals with G6PD deficiency exhibit higher MCV and MCH values. Additionally, blood donated by carriers of thalassaemia or G6PD deficiency does not increase the risk of adverse transfusion reactions. The transfusion of blood from donors with thalassaemia or G6PD deficiency showed no significant difference in haemoglobin elevation compared to that from normal donors.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":"20-27"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145507410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A blood safety perspective on emerging arboviral infections in the United Kingdom.","authors":"Piya Rajendra, Shannah Secret, Su Brailsford, Tanya Golubchik, Peter Simmonds, Heli Harvala","doi":"10.1111/tme.70041","DOIUrl":"10.1111/tme.70041","url":null,"abstract":"<p><p>A core focus of the blood services is to maintain the blood supply whilst simultaneously being vigilant for potential threats to blood safety. At present, West Nile virus (WNV), Usutu virus (USUV), Dengue virus (DENV) and Tick-borne encephalitis virus (TBEV) are considered primary arboviral threats to blood safety in the UK and Northern Europe. Climate change and globalisation have enhanced the frequency of WNV and DENV cases being reported in Europe, furthering the likelihood of their spread to the UK. Furthermore, both TBEV and USUV have already been identified in reservoir hosts in England and the first human cases of TBEV infections acquired in England have been recently documented. Existing policy to protect the blood supply against emerging viral risks is based on donor deferral or nucleic acid test (NAT) screening for those recently returning from WNV endemic areas, only. Constant evaluation of the current policy is necessary to assess the feasibility of donor deferral if the case numbers within Europe continue to increase, and to determine if selective screening for these viruses is needed. Regardless of the testing and prevention strategies decided upon by the blood services, frequent review of these policies will be necessary to reflect the national and wider disease epidemiology of these arboviral infections.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":"10-19"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12882747/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145446041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality of platelet concentrates after three-day storage following 265 nm ultraviolet C-light-emitting diode irradiation.","authors":"Tomoya Hayashi, Yoshihiko Sakurai, Yoshihiro Fujimura, Kumiko Oguma, Yuichi Mishima, Fumiya Hirayama, Yoshihiko Tani, Yoshihiro Takihara, Takafumi Kimura","doi":"10.1111/tme.70025","DOIUrl":"10.1111/tme.70025","url":null,"abstract":"<p><strong>Objectives: </strong>We aimed to investigate the quality of platelets after storage following irradiation with ultraviolet C (UVC) light-emitting diodes (LED).</p><p><strong>Background: </strong>Controlling transfusion-related infections, particularly bacterial contamination of platelet concentrates (PCs), is urgently required. UVC-LEDs have attracted considerable attention as potential solutions to this problem.</p><p><strong>Methods: </strong>PCs (5.5 mL) were irradiated with 265 nm UVC-LED for up to 60 min and then stored at 22°C with shaking for 3 days. PC quality parameters, such as platelet count, biochemical profiles, including electrolytes and metabolism, activation markers and platelet aggregability, were analysed before and after storage. Prior to the storage study, to validate the appropriateness of the UVC-LED dose used, the PCs were inoculated with Staphylococcus aureus or Bacillus cereus, and their colony-forming ability was evaluated after irradiation with the same dose of UVC-LED.</p><p><strong>Results: </strong>We confirmed that S. aureus and B. cereus colonies decreased with the irradiation dose (by 1.7 log and 1.2 log at 38.7 and 40.4 mJ cm^-2, respectively). Platelet count decreased immediately after 60-minute irradiation to 32.5 mJ cm^-2, reaching approximately 80% of the level in the control without irradiation, but no further decrease was recorded after storage. The biochemical profiles and activation markers showed little alterations.</p><p><strong>Conclusion: </strong>These results indicate that UVC-irradiated platelets maintain sufficient quality for practical use even after storage. Although this was a bench-scale study, our findings suggest that irradiation of PCs with 265 nm UVC-LED may enhance the safety of blood transfusions while preserving their efficacy.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":"45-52"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12882763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145252864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}