Transfusion Medicine最新文献

{"title":"Differential regulation of erythropoiesis-related microRNAs in blood donors: Erythrocyte glutathione predicts miR-142 expression.","authors":"Aziz Karaca, Şenol Çitli, Medeni Arpa, Eda Yılmaz Kutlu","doi":"10.1111/tme.70088","DOIUrl":"https://doi.org/10.1111/tme.70088","url":null,"abstract":"<p><strong>Background: </strong>Repeat blood donation affects erythrocyte health through physiological adaptations. This study examines how repeat donation affects erythrocyte morphology, oxidative stress and erythropoiesis-regulating microRNAs (miRNAs: miR-142, miR-144, miR-451).</p><p><strong>Methods: </strong>A cross-sectional study included 42 male participants: repeat donors (n = 21, ≥3 donations/year) and first-time donors (n = 21). Whole blood miRNA expression was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR), hemogram parameters by autoanalysers and oxidative markers (malondialdehyde [MDA], glutathione [GSH]) spectrophotometrically. A Directed Acyclic Graph was constructed for variable selection, and separate linear regression models with Gaussian error distribution were fitted for each miRNA in repeat and first-time donor groups, including 2-year donation count, age, body mass index, smoking, erythrocyte morphology (haemoglobin, mean corpuscular volume, red cell distribution width) and oxidative stress parameters (erythrocyte GSH, plasma GSH, erythrocyte MDA, plasma MDA) as predictors.</p><p><strong>Results: </strong>Univariate analysis revealed a significant 3.17-fold upregulation of miR-142 in repeat donors (p = 0.040); miR-144 and miR-451 remained stable. Linear regression analysis identified two key findings: (1) eGSH was a significant predictor of miR-142 expression in first-time donors (β = -0.080, p = 0.041), representing a fundamental redox-miRNA relationship independent of donation status; and (2) smoking approached significance as a predictor of miR-144 in repeat donors (β = 2.819, p = 0.053), approaching significance and suggesting a possible cumulative oxidative stress threshold. No predictor reached significance for miR-451 in either group, confirming its homeostatic stability.</p><p><strong>Conclusions: </strong>Repeat blood donation is associated with significant miR-142-3p upregulation. Linear regression analysis reveals a novel eGSH-miR-142 axis as a fundamental erythrocyte physiological mechanism and provides suggestive evidence for a double-hit hypothesis for miR-144 regulation in smoking donors. miR-451 demonstrates donor-independent homeostatic stability with potential as a blood product quality reference marker.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isoagglutinin dynamics and transfusion dependency after ABO-incompatible haematopoietic stem cell transplants.","authors":"C C Archana, Suryatapa Saha, Shashank Ojha, Navin Khattry, Sachin Punatar, Anant Gokarn, Akanksha Chichra, Sumeet Mirgh, Gourav Bain, Libin Mathew, Amol Tirlotkar","doi":"10.1111/tme.70080","DOIUrl":"https://doi.org/10.1111/tme.70080","url":null,"abstract":"<p><strong>Background: </strong>ABO incompatibility is common in allogeneic haematopoietic stem cell transplantation (HSCT) and may delay erythroid recovery and prolong transfusion support. Data correlating post-transplant isoagglutinin kinetics with transfusion dependency is limited.</p><p><strong>Methods: </strong>We retrospectively analysed ABO-incompatible allogeneic HSCTs performed between June 2008 and December 2023. Patients were classified as major, minor or bidirectional incompatibility. Serial Immunoglobulin M (IgM) and Immunoglobulin G (IgG) anti-A and anti-B isoagglutinin titres were monitored post-transplant. Time to isoagglutinin disappearance, donor red blood cell (RBC) appearance and associations with packed red blood cell (PRBC) and single donor platelet (SDP) transfusion requirements were evaluated.</p><p><strong>Results: </strong>Among 459 allogeneic HSCTs, 183 (39.9%) were ABO-incompatible. Median isoagglutinin disappearance was 28 days in major and 38.5 days in bidirectional incompatibility, with delayed donor RBC appearance in the latter. Anti-A isoagglutinins persisted longer than anti-B. Persistent isoagglutinins correlated with increased early PRBC and SDP transfusion requirements.</p><p><strong>Conclusions: </strong>Isoagglutinin kinetics vary by ABO incompatibility type and are associated with early transfusion dependency after HSCT.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A retrospective analysis of the antigen-negative red blood cell supply conducted at a single centre in China.","authors":"Aiping Zhao, Xiujiao Jiang, Li An, Yuanfeng Chen, Weidong He, Qun Xu, Wenchao Li, Yingfang Pan","doi":"10.1111/tme.70086","DOIUrl":"https://doi.org/10.1111/tme.70086","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to analyse the distribution and demand patterns of antigen-negative red blood cells (RBCs) in Shandong Province, China (2022-2024), with a focus on ABO blood groups and clinically significant antigens. The research was designed to provide rigorous data for advancing precision transfusion protocols and to establish a theoretical basis for optimising regional blood-sharing platforms and facilitating their expansion into a nationwide network. Furthermore, analysis of blood antigen distribution characteristics in broader populations was conducted to validate the feasibility of future expansion plans.</p><p><strong>Methods: </strong>Retrospective data from the Tangshan Qiao 9.5 Modern Blood Management System and clinical transfusion service software (2022-2024) were analysed using Excel 2010 and GraphPad Prism 10. The key metrics included ABO blood type proportions, demand intensity (the ratio of special crossmatched blood to total RBC units) and demographic trends.</p><p><strong>Results: </strong>Type B blood exhibited a 'double-high' pattern (42.76% absolute supply, 1.41% demand intensity), whereas type AB showed elevated demand intensity (1.08%) despite a low total volume supply (10.80%). The Rh (57.90%) and Kidd (22.84%) systems dominated the antigen-negative RBC distribution. Females (63.25%) and patients aged >50 years (65.73%) were the primary recipients, linked to pregnancy-related immune exposure and chronic comorbidities.</p><p><strong>Conclusion: </strong>Routine testing for RhCcEe and Kidd antigens, dynamic inventory strategies for high-demand blood types and regional data-sharing platforms are critical for increasing transfusion safety. Multicentre collaboration and the adoption of international antigen-matching protocols are recommended to address China's ageing population and chronic disease burden.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147782473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimising the workflow for infant ABO blood typing: The role of automated analyser and a manual reflex protocol.","authors":"Eunhui Ji, Seung Hwan Oh, Sun Min Lee, In-Suk Kim, Hyun Ji Lee","doi":"10.1111/tme.70081","DOIUrl":"https://doi.org/10.1111/tme.70081","url":null,"abstract":"<p><strong>Objectives: </strong>We aimed to optimise the infant ABO typing workflow by evaluating the performance of the ORTHO VISION analyser (VISION; QuidelOrtho, San Diego, CA, USA) and establishing a targeted manual reflex protocol.</p><p><strong>Background: </strong>The application of automated analysers in infants poses challenges due to weak antigen and antibody expression.</p><p><strong>Methods and materials: </strong>We reviewed ABO blood typing results from 1178 infants who underwent routine dual testing using both the VISION and the manual tube method at our centre. Samples were grouped by age: Group 1 (under 4 months, N = 898, forward typing only) and Group 2 (4 months to under 1 year, N = 280, both forward and reverse typing).</p><p><strong>Results: </strong>The VISION showed almost perfect agreement with the manual method (weighted kappa >0.94). In Group 1, the manual method showed a higher conclusive rate (100% vs. 98.7%; p < 0.001), resolving 12 technical flags. In Group 2, the VISION demonstrated a higher conclusive rate (71.4% vs. 66.4%, p = 0.006) due to superior sensitivity in detecting early anti-A antibodies (p < 0.001). Inconclusive results in Group 2 correlated significantly with younger age (p < 0.001) but not sex or diagnosis. The proposed algorithm is estimated to reduce the manual workload by 92.2% compared to the previous dual-protocol.</p><p><strong>Conclusion: </strong>The VISION is a reliable alternative to manual testing for infant ABO blood typing. Our proposed targeted reflex algorithm can minimise operational fragmentation and systemic human error, thereby optimising laboratory efficiency while maintaining patient safety.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147782517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of fibrinogen concentrate in coagulation testing aids in the diagnosis and management of rare bleeding disorders.","authors":"Yang Yang, Lulu Zhang, Zekai Lei, Ning Tang","doi":"10.1111/tme.70085","DOIUrl":"https://doi.org/10.1111/tme.70085","url":null,"abstract":"<p><strong>Background: </strong>Many clinical laboratories lack the methods or resources to quickly identify rare haemorrhagic diseases, such as genetic/acquired fibrinogen defects and factor XIII deficiency.</p><p><strong>Case report: </strong>This report explored the value of human fibrinogen concentrate in the diagnosis and management of rare bleeding disorders through three case studies. Case 1 involved acquired hypofibrinogenemia caused by snake bite, where the coagulation abnormality was corrected by adding fibrinogen in vitro, confirming the mechanism of snake venom-specific degradation of fibrinogen. Case 2 utilised fibrinogen solution to establish a thromboelastography mixing test for rapid diagnosis of autoimmune factor XIII inhibitors. Case 3 improved platelet aggregation function in patients with congenital abnormal fibrinogenaemia through fibrinogen solution.</p><p><strong>Conclusion: </strong>These case studies showed that fibrinogen concentrate may serve as a specific additive to assist in the rapid identification of coagulation dysfunction and guide precise treatment.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147780946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Timing of intrapartum transfusion among obstetric patients, stratified by admission and discharge haemoglobin, relative to anaesthesia care: A retrospective cohort study.","authors":"Franklin Dexter, Richard H Epstein, Unyime S Ituk","doi":"10.1111/tme.70082","DOIUrl":"https://doi.org/10.1111/tme.70082","url":null,"abstract":"<p><strong>Background: </strong>We previously validated and implemented criteria for auditing anaesthesiologists with intraoperative transfusion based on low median blood loss of the surgical procedure or absence of anaemia before transfusion. However, obstetrical cases were excluded during implementation, as the peripartum blood management protocol does not include haemoglobin determinations, matching American College of Obstetricians and Gynaecologists recommendations. For non-obstetrical surgery, intraoperative transfusion is associated with an increased probability of postpartum transfusion. Because of the obstetrical transfusion approach, we hypothesized the opposite relationship between intrapartum and postpartum transfusion.</p><p><strong>Methods: </strong>The retrospective cohort study included all 30 160 patients who delivered at the University of Iowa January 2013 through March 2025.</p><p><strong>Results: </strong>Any RBC administration during anaesthesia care reduced the odds of later transfusion (odds ratio 0.14, 99% confidence interval (CI) 0.11%-0.18%). Single-unit RBC administration during anaesthesia reduced such odds (0.09, 99% CI 0.06%-0.11%), as did multiple-unit transfusion (0.18, 99% CI 0.13%-0.24%). McNemar tests had Bonferroni-adjusted p-values <0.0001. While antepartum anaemia (haemoglobin <11 g/dL) increased the probability of transfusion by 7.41% (standard error 0.43%) outside of anaesthesia care, the RBC transfusion decision during anaesthesia care was less influenced by anaemia, 1.52% (0.22%), p < 0.0001. Many (59%, 99% CI 50%-69%) RBC units started during anaesthesia care were administered to patients who were not anaemic (haemoglobin ≥11 g/dL) at admission. Most patients with single-unit RBC transfusion and blood loss <2500 mL had stable haemodynamics.</p><p><strong>Conclusions: </strong>Unlike several categories of non-obstetrical procedures, RBC transfusion during anaesthesia care for delivery was associated with a reduced probability of postpartum RBC transfusion. A reasonable approach for blood bank auditing of anaesthesiologists' obstetric RBC transfusion decision-making would be to review cases when RBC were administered, there was blood loss <2500 mL, no subsequent transfusion, and discharge haemoglobin ≥9 g/dL.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2026-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147782143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Timely geographic access to blood banking facilities: A pan-India modelling study.","authors":"Gaurav Urs, Aamir Miyajiwala, Ankit Raj, Siddhesh Zadey","doi":"10.1111/tme.70075","DOIUrl":"https://doi.org/10.1111/tme.70075","url":null,"abstract":"<p><strong>Background: </strong>Efficient blood transfusion planning requires knowledge of the distribution and accessibility of blood banking facilities (BBFs). We mapped population-level, timely geographic access to BBFs across 735 districts across India.</p><p><strong>Study design and methods: </strong>We conducted a pan-India geospatial modelling study using 2024 data from e-RaktKosh, a centralised blood management system. As outcomes, we estimated BBFs per million people, travel time to the nearest BBF, and access population coverage (APC), defined as the proportion of the population with access to the nearest BBF by motorised transport (60 min) and walking (30 min).</p><p><strong>Results: </strong>In 2024, India had 5679 BBFs, averaging 4.13 facilities per million people. Median travel times to the nearest BBF were 35.25 min by motorised transport and 253.27 min by walking. For APCs, 93.86% of people were within 60 min of their nearest BBF by motorised transport, while 16.21% were within 30 min by walking. There were large differences in APCs across districts and states, with notable rural-urban disparities.</p><p><strong>Discussion: </strong>Access to blood banking facilities in India exhibits geographic disparities, with the northeastern states and island Union Territories having prolonged median travel times and low APC values. Rural areas are disproportionately adversely affected. Future efforts should focus on using timely geographic access data to inform strategic infrastructure planning and resource allocation, enhancing healthcare equity.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147782089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryoprecipitate utilisation, shortages and institutional policies in the United States: A survey of practices and policies.","authors":"Chinelo P Onyenekwu, Mariam Ratiani, Jenna Wade, Angela Treml","doi":"10.1111/tme.70079","DOIUrl":"https://doi.org/10.1111/tme.70079","url":null,"abstract":"<p><strong>Background: </strong>Since the COVID-19 pandemic, demand for blood products has remained high, while donations have declined. Institutional policies guiding the use of these products may affect their availability, utilisation and waste. We surveyed blood banks (BBs) in the United States (U.S.) to assess policies and practices related to cryoprecipitate compatibility and to characterise experiences with administration, shortages and waste.</p><p><strong>Methods: </strong>An 11-question online survey was distributed to U.S. BBs via Qualtrics. Responses were exported to Excel and analysed using descriptive statistics. Variables are reported as frequencies and percentages.</p><p><strong>Results: </strong>Of 241 BBs, 82 responded (34%). Thirty-seven (45%) served community hospitals, 31 (38%) served academic hospitals and 55 (69%) served both adult and paediatric populations. Thirty (38%) BBs reported preferentially administering ABO type-specific cryoprecipitate when available, although none reported restricting transfusion exclusively to type-specific units under all circumstances. Eighteen (23%) limited the number of out-of-group units per patient within 24 h. Ten (13%) BBs reported shortages occurring at least 3-5 times per year. During shortages, 25 (61%) BBs proactively screened orders, and 14 (34%) used fibrinogen concentrate as an alternative. Annual wastage varied, with 12 (15%) BBs discarding more than 50 pooled units, often because unused units were thawed for massive transfusion protocols (MTPs). Strategies to reduce wastage included thawing units later in the MTP cycle, proactive order screening and supplementation with fibrinogen concentrate.</p><p><strong>Conclusion: </strong>Although a subset of BBs reported substantial shortages of cryoprecipitate, some institutions reported significant waste. Targeted strategies, such as limiting units administered, proactively reviewing orders, offering alternative products and modifying thawing policies for MTPs, may improve efficiency and reduce waste.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147634426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liberal versus restrictive transfusion in haemato-oncological patients undergoing autologous stem cell transplantation: A single-centre experience.","authors":"Ayman El Idrissi, Irene M L W Körver-Keularts, Ron J M H E Straat, Erik A M Beckers","doi":"10.1111/tme.70078","DOIUrl":"https://doi.org/10.1111/tme.70078","url":null,"abstract":"<p><strong>Objectives: </strong>Assess the safety and efficacy of restrictive transfusion in haemato-oncological patients undergoing autologous haematopoietic stem cell transplantation (AHSCT).</p><p><strong>Background: </strong>Restrictive transfusion is increasingly advocated within transfusion medicine to prevent over-treatment, lower transfusion-related adverse events and decrease healthcare costs. Yet there is a paucity of evidence regarding safe restrictive transfusion strategies in AHSCT patients.</p><p><strong>Methods: </strong>Double-unit transfusion at a haemoglobin threshold of 8 g/dL (i.e., liberal transfusion) is compared to single-unit transfusion at a haemoglobin threshold of 7 g/dL (i.e., restrictive transfusion) by means of an interrupted time series study. RBC utilisation, survival and the occurrence of complications were assessed for the initial hospitalisation period and at the hundredth day following AHSCT.</p><p><strong>Results: </strong>A group of 28 liberally transfused patients is compared to 18 restrictively transfused patients. Restrictively transfused patients received a median of 0.0 RBC units (IQR 0.0-1.0) and liberally transfused patients 2.0 RBC units (IQR 0.0-4.0) during hospitalisation (U = 127.5, p = 0.002). The number of RBC concentrates administered by day +100 remained significantly lower for restrictively (0.0 units, IQR 0.0-3.0) as opposed to liberally (2.0 units, IQR 0.0-4.0) transfused patients (U = 168.5, p = 0.026). Survival and readmission rates did not differ significantly between both groups. The most prevalent complications, regardless of the applied transfusion protocol, included mucositis, fever of unknown origin and various infections.</p><p><strong>Conclusion: </strong>Restrictive transfusion through combined lowering of the haemoglobin trigger to 7 g/dL and single-unit transfusion effectively decreases RBC utilisation in a homogenous cohort of haemato-oncology patients undergoing AHSCT without hampering safety.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147628765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transfusion management in autoimmune haemolytic anaemia patients: A 5-year experience from a tertiary care referral centre in India.","authors":"Suhasini Sil, Vineet Sharma, Poonam Coshic, Hem Chandra Pandey, Apalak Garg, Chippy C S, Seema Kumari Meena, Suganya Palanisamy, Vidushi Gupta","doi":"10.1111/tme.70033","DOIUrl":"10.1111/tme.70033","url":null,"abstract":"<p><strong>Objectives: </strong>The study aimed to analyse transfusion practices in sensitised autoimmune haemolytic anaemia (AIHA) patients and transfusion outcomes following transfusion of antigen-matched best-matched (AMBM) red cells.</p><p><strong>Background: </strong>Transfusion practices in AIHA patients across resource-poor settings remain a challenge. The outcome of transfusions following the practice of transfusing best-matched (BM) units remains unknown. The data on alloimmunisation rates in sensitised AIHA patients, the utility of adsorption, and transfusion of AMBM red cells from resource-poor settings will enhance the adoption of optimal practices.</p><p><strong>Methods and materials: </strong>Retrospective data on AIHA patients' work-up were collected and analysed for a 5-year interval. Patients were grouped based on the presence or absence of alloimmunisation and whether they received transfusion of AMBM versus BM units based on serological testing. Inter-transfusion interval (ITI) and post-transfusion haemoglobin increment (PTHI) were calculated and compared.</p><p><strong>Results: </strong>Of 368 AIHA patients during the study period, adsorption was performed in 138 patients with a history of a sensitising event. Red cell alloantibodies were identified in 74 patients (53.6%). Shorter ITI was observed in alloimmunised patients when transfused BM red cells versus AMBM red cells [2 (1-3) days vs. 4 (3-5) days; p < 0.001]. Lower PTHI was observed in alloimmunised patients when transfused BM red cells versus AMBM red cells [0.65 (0.45-0.8) g/dl vs. 0.9 (0.7-1.2) g/dL; p < 0.001].</p><p><strong>Conclusion: </strong>High alloimmunisation rates were observed in sensitised AIHA patients. Significantly higher PTHI and longer ITI were observed with transfusion of AMBM units. These findings highlight the benefits of identifying and matching against alloantibodies for better transfusion outcomes.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":"142-149"},"PeriodicalIF":1.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145281026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}