Transfusion Medicine最新文献

{"title":"Recruiting racialised youth to a stem cell registry in Canada.","authors":"Kelly Holloway, Ufuoma Muwhen, Warren B Fingrut, Jennie Haw","doi":"10.1111/tme.13155","DOIUrl":"https://doi.org/10.1111/tme.13155","url":null,"abstract":"<p><strong>Objectives: </strong>The aim of this paper is to report results of racialised young adults' awareness of the stem cell registry. Further, we examine recruitment materials and outreach strategies to increase stem cell awareness and registration among young, racialised Canadians.</p><p><strong>Background: </strong>Patients who are not White face disparities in securing unrelated donors because of the disproportionate representation of White registrants on stem cell registries, differences in genetic diversity across ethnicities, and attrition rates for donor registries.</p><p><strong>Methods/materials: </strong>This qualitative study is informed by community-based participatory research. Focus groups were conducted with young adults ages 17-35 who self-identify as Black, Indigenous, and people of colour (BIPOC)/racialised, are comfortable speaking in English, and live in Canada.</p><p><strong>Results: </strong>Five 2-h focus groups were conducted with 17 participants in total. Participants spoke to the interconnected themes of knowledge, trust, and engagement with their community. They were motivated by the idea of contributing to a more diverse registry. These perspectives informed their insight into what materials and events would resonate with their peers and their communities.</p><p><strong>Conclusion: </strong>Our findings point to some concrete suggestions for materials that can resonate with young racialised Canadians. Further, stem cell registries should work collaboratively with young adults and young adult organisations to co-develop educational and recruitment materials and build relationships over time, developing their knowledge of stem cells and raising their confidence to host stem cell events within their communities.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracorporeal photopheresis-New insights into an old procedure.","authors":"Sabine Seiffert, Janine Kirchberg, Enrica Bach, Victoria Menger, Mandy Brückner, Ulrich Sack, Ulrike Köhl, Uwe Platzbecker, Andreas Boldt, Marco Herling, Vladan Vučinić","doi":"10.1111/tme.13156","DOIUrl":"https://doi.org/10.1111/tme.13156","url":null,"abstract":"<p><strong>Background: </strong>Extracorporeal photopheresis (ECP) is a safe immunomodulatory strategy that induces cell-type selective apoptosis through photodynamic processes. Despite decades of use, the mechanisms underlying ECP remain largely unexplored, particularly in studies examining specific immune cell subsets in ex vivo setups.</p><p><strong>Aims: </strong>This proof-of-concept pilot study presents data on apoptosis and proliferation of T-lymphocytes following ex vivo ECP application to leukocyte concentrates (LC) and peripheral blood (PB) samples from healthy donors.</p><p><strong>Methods: </strong>LC and PB were diluted to a haematocrit of 2% and treated with 8-methoxypsoralen, followed by ECP (ECP+) or no ECP (ECP-) in a discontinued system. Apoptosis of mononuclear cells was assessed 48 h post-ECP using annexin V and 7 Aminoactinomycin D (7-AAD) staining with flow-cytometric quantification. The proliferative capacity of non-apoptotic T-lymphocytes was measured after 72 h of post-ECP stimulation with anti-CD3/CD28 cross-linking, using Violet Proliferation Dye 450.</p><p><strong>Results: </strong>ECP exposure significantly reduced the median T-cell receptor-induced proliferation of viable T-lymphocytes from both LC (4.6%, p = 0.02) and PB (4.2%, p = 0.03). However, 7-AAD staining 48 h post-ECP showed no significant differences in the proportions of apoptotic cells in this experimental model.</p><p><strong>Conclusion: </strong>Ex vivo ECP treatment inhibited T-lymphocyte proliferation in both LC and PB from healthy individuals, suggesting this as a key mode of action. Our findings highlight ECP's potential applications, including its implications for modern immune therapies' adverse effects. Further analyses of functional characteristics of remaining vital cells are necessary.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance of a large Brazilian network of blood banks during the COVID-19 pandemic: Impact assessment and time series analysis (2016-2023).","authors":"Maria Clara F Silva-Malta, Elias M Oliveira, Fabiana C C Piassi, Maisa A Ribeiro, Kátia C Coelho, Daniela O W Rodrigues, Ricardo V F Carvalho, Junia G M Cioffi, Marina L Martins","doi":"10.1111/tme.13152","DOIUrl":"https://doi.org/10.1111/tme.13152","url":null,"abstract":"<p><strong>Background: </strong>During the COVID-19 pandemic, blood banks faced the risk of shortages of blood components and adopted measures to mitigate this threat. The present study aims to describe the influence of the pandemic on a large hemotherapy centre, including data from 21 regional blood centres (Hemominas-Minas Gerais/Brazil).</p><p><strong>Methods: </strong>Time series for the blood donor attendance were constructed covering 8 years (2016-2023). Blood centre performance indicators from the pandemic period (2020-2023) were compared with the pre-pandemic period (2016-2019).</p><p><strong>Results: </strong>During the pandemic, a 11.6% decline in the production of blood components was observed (Semiannual average of 355 511 vs. 402 528 units). The first half of 2022 was the period with the highest number of COVID-19 cases (third wave) and the lowest production. The drop in the number of candidates for blood donation was more pronounced in the most populated cities. An increase in returning donors was observed, as well as a decrease in the deferral rate. The time series analysis indicated a strong downward trend in blood donors during the pandemic period but with a tendency to recover from the second half of 2023.</p><p><strong>Conclusion: </strong>The COVID-19 pandemic significantly affected the hemotherapy system in Minas Gerais, resulting in a drop in the production of blood components. The operation of Hemominas as a network of blood centers contributed to mitigating the effects of the pandemic, alleviating the scarcity of blood components, especially in the most populated cities, where blood donation was very affected and where the largest and most complex hospitals are located.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Practice of ABO antibody titration in a transplant center: From tube method to manual gel column testing to automated column agglutination technology.","authors":"Jun-Bo Sun, Li-Wen Chen, Li-Li Tao, Qing-Si Wu, Qian-Qian Yu, Zhi-Wei Xie","doi":"10.1111/tme.13127","DOIUrl":"10.1111/tme.13127","url":null,"abstract":"<p><strong>Background and objectives: </strong>High-quality ABO antibody titre testing is required for ABO-incompatible haematopoietic stem cell transplantation and kidney transplantation. To assess the feasibility of automated ABO titration as an alternative to manual and semi-automatic titration during the peri-transplant period, a comparative study was conducted internally in a transfusion medicine laboratory.</p><p><strong>Materials and methods: </strong>This study was performed in two stages. Firstly, the differences in anti-A/B detecting using conventional tube test (CTT) and manual column agglutination technology (CAT) were compared in group O pregnant women. Then, manual and automated CAT were applied to simultaneously detect ABO antibody levels in non-AB-group adult haematological oncology patients.</p><p><strong>Results: </strong>In the first stage, four groups of 192 comparable results were generated from 48 subjects, which showed a high correlation between CTT and manual CAT (p < 0.001). However, the detection sensitivity of the latter was at least 1 fold higher dilution than that of the former. Fifty-six patients were tested in the second stage by simultaneous manual and automated CAT, and the paired titers differed within an acceptable range (±1 fold dilution). However, the concordance was slightly higher in group IgM (85.4%) than in group IgG (80.5%). Blood group specificity of ABO titers was also observed in this population, and no association between titers and other factors was found.</p><p><strong>Conclusion: </strong>Automated CAT standardises ABO titre testing and saves labor costs, although manual review of weak positive reactions is still necessary. Due to the blood group specificity of ABO antibodies, special attention should be paid to the quality control and clinical relevance of titre testing for group O recipients in ABO-mismatched transplantation.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":"258-265"},"PeriodicalIF":1.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of conventional coagulation tests and ROTEM in identifying trauma-induced coagulopathy for massive haemorrhage protocol activation.","authors":"Sakara Hutspardol, Tyrone Borja, Jenna Kroeker, Xiu Qing Wang, Jian Mi, David Zamar, Geoffrey Chan, Tyler Smith, Harvey Hawes, Andrew W Shih","doi":"10.1111/tme.13128","DOIUrl":"10.1111/tme.13128","url":null,"abstract":"<p><strong>Objectives: </strong>Trauma-induced coagulopathy (TIC) can be fatal but preventable if recognised early. With emerging uses of rotational thromboelastometry (ROTEM) to guide transfusions in trauma, patient outcomes with TIC-defined by initial ROTEM and conventional coagulation tests (CCTs) during massive haemorrhage protocol (MHP) activations were evaluated at a primary trauma centre in British Columbia.</p><p><strong>Methods: </strong>This retrospective observational study included adult trauma patients requiring MHP from June 1, 2020, to May 31, 2022. TIC, defined by initial results including (1) ROTEM-based EXTEM A10 <40 mm, EXTEM CT >100 s, EXTEM ML30 >10%, FIBTEM A10 <10 mm; and (2) CCT-based INR ≥1.8, PTT ≥1.5 times of upper normal limit, platelets <50 x 10⁹/L, and Clauss Fibrinogen <1.5 g/L, was assessed for its correlation with mortality. Modified Poisson regression was used to model 28-day mortality.</p><p><strong>Results: </strong>Twenty-two of sixty-eight patients (32%) had abnormal ROTEM but normal CCTs. TIC defined by CCTs was associated with increased mortality [24 h: 5/13 (38%) vs. 5/55 (9%), p = 0.025; 28d: 8/13 (62%) vs. 11/55 (20%), p = 0.002]; compared to ROTEM, which was not [24 h: 7/35 (20%) vs. 3/33 (9%), p = 0.307; 28d: 11/35 (31%) vs. 9/33 (27%), p = 0.594], despite requiring significantly higher blood component transfusion within the first 4 and 24 h of MHP (p-values<0.05).</p><p><strong>Conclusions: </strong>ROTEM is more sensitive in identifying TIC. Patients with abnormal CCTs had a higher death rate, and those with abnormal ROTEM had no significantly increased mortality. A prospective study is required to assess the effects of ROTEM further.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":"266-274"},"PeriodicalIF":1.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of an indigenous questionnaire for assessing clinician's knowledge towards transfusion medicine and bedside transfusion practices.","authors":"Anubhav Gupta, Hari Krishan Dhawan, Romesh Jain, Ratti Ram Sharma, Vipin Kaushal, Amarjeet Singh, Neelam Marwaha","doi":"10.1111/tme.13130","DOIUrl":"10.1111/tme.13130","url":null,"abstract":"<p><strong>Introduction: </strong>Due to the non-availability of formal training during residency for clinicians in transfusion medicine, there is a prevalent knowledge gap for transfusion practices, which leads to increased patient risks and elevated healthcare costs. There is no indigenous questionnaire available, which could be used for knowledge assessment. This study aims to develop and validate an Indigenous questionnaire to assess clinicians' knowledge of transfusion medicine and bedside transfusion practices.</p><p><strong>Materials and methods: </strong>The questionnaire was designed using a nominal group technique involving subject experts, considering the type of information required, themes, appropriate wording, layout, and presentation. The questionnaire consisted of 25 knowledge-based questions and 4 general questions. Piloting of the questionnaire was done to check for feasibility, validation, and reliability. Content validity was assessed by six experts using the Content Validity Index (CVI). Reliability was assessed using test-retest and split-half methods, with a sample of 56 participants. Cronbach's alpha and Kappa statistics were used to measure internal consistency and agreement, respectively.</p><p><strong>Results: </strong>The questionnaire displayed acceptable feasibility with a mean difficulty score of 6.93 on a scale of 1-10, with test-retest responses showing near-perfect agreement (kappa value 0.8-0.99). All experts gave more than 70% agreement on the relevance of content, with a mean CVI of 85%. Test-retest reliability showed near-perfect agreement (Kappa 0.8-0.99, p = 0.008) and good internal consistency (Cronbach's α = 0.806). The split-half method yielded a Cronbach's α of 0.89 and an intraclass correlation coefficient of 0.88 (95% CI 0.57, 0.97, p = 0.001) Construct validity was confirmed through factor analysis.</p><p><strong>Conclusion: </strong>The developed indigenous questionnaire is a reliable and valid tool for assessing the knowledge of clinicians towards transfusion medicine and bedside transfusion practices. The detailed, methodical strategy used to prepare and validate the questionnaire ensures its applicability and relevance in various clinical settings and can be easily adopted by others intending to prepare similar questionnaires. The questionnaire is available with the author and, on demand, may be provided for knowledge assessment.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":"215-225"},"PeriodicalIF":1.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The experience of the Turkish Red Crescent blood banks in the 2023 Türkiye earthquake.","authors":"Aziz Karaca, Levent Sağdur, Mustafa Nuri Günçıkan, Mustafa Yılmaz, Nazlı Sözmen, Kerem Kınık, Fatma Meriç Yılmaz","doi":"10.1111/tme.13129","DOIUrl":"10.1111/tme.13129","url":null,"abstract":"<p><strong>Aim: </strong>This cross-sectional study aimed to determine the impact of a severe earthquake on blood donation as per blood safety and donation behaviour.</p><p><strong>Materials and methods: </strong>This retrospective cross-sectional study included a total of 263 779 whole-blood donors at the Turkish Red Crescent during two periods: before (25 January 2023 - 5 February 2023) and after (6 February 2023 - 17 February 2023) the earthquake that occurred on 6 February 2023. Demographic data describing the donors, donor types, the number of blood donations by province, blood donations by blood groups, hospital demand, and transfusion transmissible infection (TTI) rates according to donor type were evaluated.</p><p><strong>Results: </strong>There was a 129% increase in blood donations after the earthquake. his increase was significant among female donors, younger age groups, and first-time donors. However, a significant increase in TTI rates was not detected in either first time donors or repeat donors.</p><p><strong>Conclusion: </strong>Our study focuses on blood banking activities of the Turkish Red Crescent during the 2023 Türkiye earthquake. While previous disasters have shown excess blood collection, we emphasise the need for a balanced approach to avoid wastage and shortages. Our study emphasises the importance of efficient blood utilisation and the necessity of regular, voluntary blood donations to ensure a stable supply, especially for high-demand blood groups like O Rh (-).</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":"235-242"},"PeriodicalIF":1.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The efficacy and safety of therapeutic thrombocytapheresis in patients with extreme thrombocytosis.","authors":"Serhat Çelik, Zeynep Tuğba Güven, Muzaffer Keklik, Ali Ünal, Leylagül Kaynar","doi":"10.1111/tme.13150","DOIUrl":"10.1111/tme.13150","url":null,"abstract":"<p><strong>Introduction: </strong>Therapeutic thrombocytapheresis (TTA) is used to promptly reduce platelet count to prevent thrombohemorrhagic complications that may develop in extreme thrombocytosis. In this study, the efficacy and safety of TTA were investigated in our patients who underwent TTA for extreme thrombocytosis for more than 20 years.</p><p><strong>Materials and methods: </strong>Forty-four patients who underwent TTA due to extreme thrombocytosis between January 2000 and December 2021 in Erciyes University, Faculty of Medicine Therapeutic Apheresis Unit were analysed retrospectively. Extreme thrombocytosis was defined as a platelet count >1000 × 10⁹/L.</p><p><strong>Results: </strong>The median percentage of platelet reduction was 62.2% (28.6%-90.1%). TTA was administered with Spectra Optia in 45.5% (n = 20) and Fresenius in 54.5% (n = 24) of the patients. No statistically significant difference was observed between age, gender, diagnoses, and devices used regarding TTA efficiency. The percentage of platelet reduction was higher in those who had a platelet precount above 1500 × 10⁹/L than those who did not (64.5% ± 9.9% vs. 57.3% ± 12.9%, p = 0.045). No major complications were observed. Mild side effects were observed in five (11.4%) patients.</p><p><strong>Conclusion: </strong>TTA provided prompt, effective, and safe reduction of platelet count in the treatment of extreme thrombocytosis.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":"253-257"},"PeriodicalIF":1.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reducing allogenic blood exposure in paediatric cellular therapy collections: A conservative prime strategy.","authors":"Andriana Pavlovich, Yamac Akgun","doi":"10.1111/tme.13151","DOIUrl":"10.1111/tme.13151","url":null,"abstract":"<p><strong>Background and objectives: </strong>Mononuclear cell (MNC) collection is critical for paediatric patients undergoing cellular therapies such as chimeric antigen receptor T-cell and haematopoietic stem cell transplantation. In children, extracorporeal volume (ECV) often exceeds 15% of total blood volume (TBV), traditionally necessitating red blood cell (RBC) priming to reduce the risk of haemodynamic instability. However, RBC priming introduces allogenic blood exposure and related complications. This study evaluated the safety and feasibility of unprimed MNC collection in paediatric patients with ECV/TBV ratios greater than 15%.</p><p><strong>Materials and methods: </strong>We retrospectively reviewed two paediatric patients with B-cell acute lymphoblastic leukaemia (B-ALL) who underwent MNC collection using the Spectra Optia system in continuous mononuclear cell collection mode. Both patients had ECV/TBV ratios of 16% and 17%, respectively. Vital signs and laboratory parameters were monitored throughout and after the procedures to assess for adverse events, haemodynamic instability, and need for transfusion.</p><p><strong>Results: </strong>Both patients completed MNC collection without adverse reactions, interruptions, or clinically significant changes in vital signs. Post-procedural haemoglobin and platelet counts showed no major declines, and neither patient required transfusion. Collection efficiency met institutional standards, and no symptoms of hypovolemia or citrate toxicity were observed.</p><p><strong>Conclusion: </strong>Unprimed MNC collection can be safely performed in paediatric patients even when the ECV/TBV ratio exceeds 15%. With careful monitoring and procedural planning, this conservative strategy may reduce allogenic blood exposure without compromising safety or collection efficiency.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":"250-252"},"PeriodicalIF":1.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors affecting the efficacy of human leukocyte antigen-selected platelet provision: A large retrospective study in the United Kingdom.","authors":"W M Howell, L Quaye, L Mumford, S Phillips, K Mepani, S Mohamed, C J Brown","doi":"10.1111/tme.13123","DOIUrl":"10.1111/tme.13123","url":null,"abstract":"<p><strong>Background: </strong>A large, retrospective study was designed to interrogate current NHS Blood and Transplant (NHSBT) HLA matching strategies for the provision of HLA selected platelets (HLA SP) and to determine whether additional factors such as ABO blood group matching, patient diagnosis, patient and/or donor age, sex, ethnicity, age of platelet unit at transfusion and possibly seasonal variation also play a role in transfusion efficacy.</p><p><strong>Materials and methods: </strong>Data for 56 640 HLA SP transfusions over a 3-year period were collected. Transfusions with missing data for any factor under consideration were excluded, resulting in a cohort of 13 044 transfusions for analysis. Univariable and multivariable regression models were used to determine if any factors influenced an increase in platelet count of ≥10 × 10⁹/L. A stepwise logistic regression was applied, such that each influential factor was adjusted for effects on other factors included in the study.</p><p><strong>Results: </strong>HLA match grade was confirmed as a significant factor in transfusion efficacy, with ABO mismatched units 20% less likely to give an adequate platelet increment (≥10 × 10⁹/L). Platelet donor age, gender and ethnicity were not significant. Conversely, patient diagnosis, ethnicity, gender and age showed significant associations with platelet increments. Some seasonal variation in efficacy of platelet transfusion was also demonstrated.</p><p><strong>Conclusion: </strong>This study has demonstrated the efficacy of HLA SP transfusions in refractory patients with a wide range of diagnoses, the importance of HLA match grade, plus the marginal effect of ABO matching. A wide range of donor-related factors was excluded, while a number of patient-related factors were identified, requiring more extensive investigation in ongoing and independent studies, with implications for donor registry, clinical and laboratory practices.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":"205-214"},"PeriodicalIF":1.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}