Transplant immunology最新文献

{"title":"Immune reconstitution and cidofovir administration rescue human adenovirus hepatitis after allogeneic hematopoietic cell transplantation","authors":"Takahiro Tomoda ,&nbsp;Akira Nishimura ,&nbsp;Takahiro Kamiya ,&nbsp;Kumi Inoue ,&nbsp;Harutaka Katano ,&nbsp;Shun Iida ,&nbsp;Akihiro Hoshino ,&nbsp;Takeshi Isoda ,&nbsp;Kohsuke Imai ,&nbsp;Michiko Kajiwara ,&nbsp;Masatoshi Takagi ,&nbsp;Hirokazu Kanegane ,&nbsp;Nozomu Hanaoka ,&nbsp;Tomohiro Morio","doi":"10.1016/j.trim.2024.102093","DOIUrl":"10.1016/j.trim.2024.102093","url":null,"abstract":"<div><p>Human adenovirus infection (HAdV) may be fatal in patients undergoing allogeneic hematopoietic cell transplantation (HCT). Cidofovir is effective in only a part of the post-HCT HAdV infection. Therefore, posttransplant immune reconstitution is important for HAdV clearance. We describe the detailed immune reconstitution and response of adenovirus-specific T cells in a patient with inborn errors of immunity who had disseminated HAdV infection with hepatitis post-HCT and was treated with cidofovir.</p><p>Though the patient received cidofovir for only 19 days starting from Day 72 after HCT because of renal dysfunction, we observed T-cell reconstitution, a decrease in HAdV copy number, and amelioration of the symptoms of HAdV infection after Day 90. We initially observed expanded NK and CD8⁺CD45RO⁺ memory subsets and later gradual increase of naïve T cells eveloped after cessation of cidofovir treatment. An increase in adenovirus-specific IFN-γ secretion from 2 to 4 months after HCT was confirmed by ELISpot assay.</p><p>The progression of immune reconstitution and cidofovir treatment are considered to have contributed to survival in this patient. Optimization of transplantation methods, prompt appropriate antiviral medication, and virus-specific T-cell therapy would be necessary as the better strategy for systemic HAdV infection.</p></div>","PeriodicalId":23304,"journal":{"name":"Transplant immunology","volume":"86 ","pages":"Article 102093"},"PeriodicalIF":1.6,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of COVID-19 on anti-HLA antibodies in kidney transplantation","authors":"Marcos Vinicius de Sousa ,&nbsp;Bruno Teixeira Gomes ,&nbsp;Ana Claudia Gonçalez ,&nbsp;Marilda Mazzali","doi":"10.1016/j.trim.2024.102092","DOIUrl":"10.1016/j.trim.2024.102092","url":null,"abstract":"<div><p>The effects of COVID-19 on the immune profile of kidney transplant recipients are unknown. Immunosuppression adjustment during the illness can increase the risk for <em>de novo</em> donor-specific anti-HLA antibodies (DSA) and acute rejection episodes. This single-center retrospective study includes adult kidney transplant recipients diagnosed with COVID-19 between March 2020 and December 2022, screened for anti-HLA antibodies (AbHLA) pre-transplant and after COVID-19. Analyzed data comprised demographics, immunosuppressive therapy before and during the illness, hospitalization rate, and AbHLA specificity. Two hundred sixty-seven transplant recipients were included and divided according to the pre-transplant AbHLA profile: absent [PRA- (<em>n</em> = 206, 77%)], non-DSA (<em>N</em> = 46, 17%), and DSA+ (<em>n</em> = 15, 6%). The DSA+ group was younger (40.5 ± 16.5; PRA- 50.3 ± 13.4; non-DSA 49.3 ± 11.7 years; <em>p</em> = 0.02). The hospitalization rate was higher in groups with preformed AbHLA (DSA+ <em>n</em> = 8, 53%; non-DSA = 24, 52%; PRA- <em>n</em> = 54, 26%; <em>p</em> &lt; 0.01). Immunosuppression was maintained in 222 (83%), withdrawn in 33 (12%), and reduced in 11 (4%) cases without difference among groups. Twenty-two (8%) cases of <em>de novo</em> DSA were observed after COVID-19 [PRA-, <em>n</em> = 16 (73%) and non-DSA, <em>n</em> = 6 (27%)]. In the DSA+ group, the AbHLA profile remained stable. There were 6 (2%) cases of post-COVID-19 antibody-mediated rejection (DSA+ <em>n</em> = 4, 66%; non-DSA <em>n</em> = 1, 17%, PRA- n = 1, 17%) without T cell-mediated rejection cases. Post-COVID-19 <em>de novo</em> DSA was more frequent in groups without pre-transplant AbHLA, not having association with changes in immunosuppressive therapy.</p></div>","PeriodicalId":23304,"journal":{"name":"Transplant immunology","volume":"86 ","pages":"Article 102092"},"PeriodicalIF":1.6,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic value of selenoprotein changes in renal tissues for acute rejection of kidney transplantation as revealed by transcriptomics","authors":"Xingyu Pan ,&nbsp;Rong Zhu ,&nbsp;Jinpu Peng,&nbsp;Hongyu Tang,&nbsp;Nini An,&nbsp;Jun Pei","doi":"10.1016/j.trim.2024.102082","DOIUrl":"10.1016/j.trim.2024.102082","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;p&gt;There seems to be a close link between the changing levels of selenoproteins, which are important for maintaining redox homeostasis in the body, and acute rejection of kidney transplants. The aim of this study was to explore the diagnostic value of selenoprotein change characteristics in renal tissues for acute rejection of kidney transplantation.&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;p&gt;We first explored the potential biological functions of 25 selenoproteins in the human body by enrichment analysis and used the HPA database to clarify the expression levels of selenoproteins in kidney tissues; We then constructed a diagnostic model using “Logistic regression analysis” and “Nomogram model”; Calibration curves and ROC curves were used to evaluate the diagnostic models, and clinical decision curves (DCA) were used to assess the diagnostic value of selenoprotein changes to the clinic; Single-gene GSEA enrichment analysis to further explore the potential regulatory mechanisms of selenoproteins; The Cibersort algorithm explores the level of immune cell infiltration and uses correlation analysis to clarify the correlation between selenoproteins and immune cells; We further assessed the diagnostic value of selenoproteins in kidney transplantation ABMR and TCMR, respectively. Finally, we validated the expression level of selenoproteins in kidney tissues by constructing a rat model of acute rejection of kidney transplantation using transcriptome sequencing.&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;p&gt;Our enrichment analysis revealed that selenoproteins are mainly closely associated with biological functions such as oxidative stress, inflammation, and immune regulation (&lt;strong&gt;P&lt;0.05&lt;/strong&gt;); The HPA database suggests that a total of 23 selenoproteins can be expressed in kidney tissue. We constructed a diagnostic model using these 23 selenoproteins, and both calibration curves and ROC curves proved that their change levels have good diagnostic value for acute rejection of kidney transplantation, and DCA curves proved the role of selenoproteins in clinical decision-making; Single-gene GSEA enrichment analysis revealed that selenoproteins are closely associated with immune regulation-related pathways (&lt;strong&gt;P&lt;0.05&lt;/strong&gt;); The Cibersort algorithm identified 10 immune cell infiltration levels that were significantly altered during acute rejection of kidney transplantation (&lt;strong&gt;P&lt;0.05&lt;/strong&gt;), while correlation analyses indicated that selenoproteins correlate with multiple immune cell infiltrations; In ABMR and TCMR, we again verified the diagnostic value of selenoprotein changes in acute rejection of kidney transplantation. Finally, we found significant differences in the expression levels of nine selenoproteins in a rat model of acute rejection of kidney transplantation (&lt;strong&gt;P&lt;0.05&lt;/strong&gt;).&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;p&gt;Changes in selenoproteins in renal tissues have good diagnostic value for acute rejection of ki","PeriodicalId":23304,"journal":{"name":"Transplant immunology","volume":"85 ","pages":"Article 102082"},"PeriodicalIF":1.6,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141604200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bushen Huoxue decotion-containing serum prevents chondrocyte pyroptosis in a m6A-dependent manner in facet joint osteoarthritis","authors":"","doi":"10.1016/j.trim.2024.102083","DOIUrl":"10.1016/j.trim.2024.102083","url":null,"abstract":"<div><h3>Background</h3><p>Facet joint osteoarthritis (FJOA) is a common lumbar osteoarthritis characterized by degeneration of small joint cartilage. Bushen Huoxue decotion (BSHXD) has good therapeutic effects on OA. Our work aimed to further probe the pharmacological effects of BSHXD-containing serum (BSHXD-CS) on FJOA and define underlying the mechanisms invovled.</p></div><div><h3>Methods</h3><p>To establish a FJOA cell model, primary rat chondrocytes were treated with LPS. The mRNA and protein expressions were assessed using qRT-PCR and western blot, respectively. The secretion levels of pro-inflammatory cytokines were measured by ELISA. Cell viability was determined by CCK8 assay. The global m⁶A level was detected by the kit, and NLRP3 mRNA m⁶A level was determined by Me-RIP assay. The molecular interactions were analyzed by RIP and RNA pull-down assays.</p></div><div><h3>Results</h3><p>BSHXD-CS treatment relieved LPS-induced cell injury, inflammation, NLRP3 inflammasome and pyroptosis in chondrocytes (all <em>p</em> &lt; 0.05). LPS-induced NLRP3 upregulation in chondrocytes was related to its high m⁶A modification level (<em>p</em> &lt; 0.05). It was also observed that BSHXD-CS reduced LPS-induced m⁶A modification in chondrocytes via repressing STAT3 (all <em>p</em> &lt; 0.05), suggesting BSHXD-CS could repress NLRP3 expression via m⁶A-dependent manner. Moreover, DAA, a m⁶A specific inhibitor, was proved to strengthen the protectively roles of BSHXD-CS on LPS-challenged pytoptosis (all <em>p</em> &lt; 0.05).</p></div><div><h3>Conclusion</h3><p>BSHXD-CS inhibited NLRP3 inflammasome activation and pyroptosis in chondrocytes to repress OA progression by reducing RNA m⁶A modification.</p></div>","PeriodicalId":23304,"journal":{"name":"Transplant immunology","volume":"86 ","pages":"Article 102083"},"PeriodicalIF":1.6,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141601877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling the potential of TIM-3 gene polymorphism in allogeneic hematopoietic stem cell transplantation - a preliminary study","authors":"Sylwia Biały ,&nbsp;Jagoda Siemaszko ,&nbsp;Małgorzata Sobczyk-Kruszelnicka ,&nbsp;Wojciech Fidyk ,&nbsp;Iwona Solarska ,&nbsp;Barbara Nasiłowska-Adamska ,&nbsp;Patrycja Skowrońska ,&nbsp;Maria Bieniaszewska ,&nbsp;Agnieszka Tomaszewska ,&nbsp;Grzegorz W. Basak ,&nbsp;Sebastian Giebel ,&nbsp;Tomasz Wróbel ,&nbsp;Katarzyna Bogunia-Kubik","doi":"10.1016/j.trim.2024.102084","DOIUrl":"10.1016/j.trim.2024.102084","url":null,"abstract":"<div><h3>Background</h3><p>T-cell immunoglobulin and mucin-domain containing-3 (TIM-3) molecule is a key regulator of the immune response by exerting an inhibitory effect on various types of immune cells. Understanding the role of TIM-3 in hematopoietic stem cell transplantation (HSCT) may improve transplant outcomes. Our study evaluated the potential association between <em>TIM-3</em> polymorphisms, namely rs1036199 (<em>A</em> &gt; <em>C</em>) or rs10515746 (<em>C</em> &gt; <em>A</em>), changes which are located in exon 3 and the promoter region of the <em>TIM-3</em> gene, and post-HSCT outcomes.</p></div><div><h3>Methods</h3><p>One-hundred and twenty allogeneic HSCT patients and their respective donors were enrolled and genotyped for <em>TIM-3</em> single nucleotide polymorphisms (SNPs) using real-time PCR with TaqMan assays.</p></div><div><h3>Results</h3><p>We found that the presence of the rare alleles and heterozygous genotypes of studied SNP in recipients tended to protect against or increase the risk for acute graft-versus-host disease (aGvHD). For the rs1036199 polymorphism, recipients with the <em>AC</em> heterozygous genotype (<em>p</em> = 0.0287) or carrying the rarer <em>C</em> allele (<em>p</em> = 0.0334) showed a lower frequency of aGvHD development along all I-IV grades. A similar association was detected for the rs10515746 polymorphism as recipients with the <em>CA</em> genotype (<em>p</em> = 0.0095) or the recessive <em>A</em> allele (<em>p</em> = 0.0117) less frequently developed aGvHD. Furthermore, the rarer <em>A</em> allele of rs10515746 SNP was also associated with a prolonged aGvHD-free survival (<em>p</em> = 0.0424). Cytomegalovirus (CMV) infection was more common in patients transplanted with <em>TIM-3</em> rs10515746 mismatched donors (<em>p</em> = 0.0229) and this association was also found to be independent of HLA incompatibility and pre-transplant CMV-IgG status. Multivariate analyses confirmed the role of these recessive alleles and donor-recipient <em>TIM-3</em> incompatibility as an independent factor in aGvHD and CMV development.</p></div><div><h3>Conclusions</h3><p>Polymorphism of TIM-3 molecule may affect the immune response in HSCT patients. The recessive alleles of rs1036199 and rs10515746 SNPs decreased the risk of developing aGvHD. <em>TIM-3</em> donor-recipient genetic matching may also affect the risk of post-transplant CMV infection, indicating the potential value of genetic profiling in optimizing transplant strategies.</p></div>","PeriodicalId":23304,"journal":{"name":"Transplant immunology","volume":"85 ","pages":"Article 102084"},"PeriodicalIF":1.6,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S096632742400100X/pdfft?md5=acdfaecb98a72ad556f23bf2a8e74970&pid=1-s2.0-S096632742400100X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141591487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction immunosuppression strategies and outcomes post-lung transplant: A single center experience","authors":"Tathagat Narula ,&nbsp;Francisco Alvarez ,&nbsp;Yousif Abdelmoneim ,&nbsp;David Erasmus ,&nbsp;Zhuo Li ,&nbsp;Mohamed Elrefaei","doi":"10.1016/j.trim.2024.102081","DOIUrl":"10.1016/j.trim.2024.102081","url":null,"abstract":"<div><h3>Purpose</h3><p>Currently 80% of lung transplant centers use induction immunosuppression. However, there is a lack of standardization of induction protocols within and across lung transplant centers. This study explores the association of two different induction immunosuppression strategies used at our center [single dose rabbit antithymocyte globulin (rATG) vs. alemtuzumab] compared to no induction with immunologic and clinical outcomes after lung transplantation.</p></div><div><h3>Methods</h3><p>A total of 174 consecutive lung transplant recipients (LTR) between 2016 and 2019 were included in the analysis. Twenty nine LTR (16.7%) received no induction, 22 LTR (12.6%) received alemtuzumab, 123 LTR (70.6%) received a single dose of rATG; 1.5 mg/kg within 24 h of transplant for induction. All LTR had a negative flow cytometry crossmatch on the day of the transplant. All LTR were assessed for de novo HLA donor-specific antibodies (DSA) development and clinical outcomes, including the risk of acute cellular rejection (ACR), antibody-mediated rejection (AMR), chronic lung allograft dysfunction (CLAD), and overall survival post-transplant.</p></div><div><h3>Results</h3><p>The median lung allocation score (LAS) was significantly higher in LTR that did not receive Induction immunosuppression <strong>(76; range = 35.3–94.3)</strong> compared to induction with rATG <strong>(41.6; range = 31.6–91)</strong> and alemtuzumab <strong>(51; range = 33.1–88.2)</strong> (<em>p</em> &lt; 0.001). De novo HLA DSA were detected in 50/174 (28.7%) LTR within 12 months post-transplant. They were detected in 13/29 (44.8%) LTR without induction immunosuppression compared to 28/123 (22.8%) and 9/22 (40.9%) LTR with rATG and alemtuzumab induction, respectively (<em>p</em> = 0.02). The percent freedom from ACR rates between LTR who received alemtuzumab induction was significantly higher compared to LTR who received rATG or no induction at 1 (<em>p</em> = 0.02), 2 (<em>p</em> = 0.01) and 3 (<em>p</em> = 0.05) years post-transplant. In addition, the overall 1-year survival rates were significantly higher in LTR who received rATG or alemtuzumab induction compared to LTR without induction immunosuppression (<em>p</em> = 0.02).</p></div><div><h3>Conclusion</h3><p>Induction immunosuppression strategies utilizing rATG or Alemtuzumab have unique and contrasting benefits in LTR. Combination of alemtuzumab induction and a lower dose of maintenance immunosuppression may reduce the incidence of ACR in LTR. Single-dose rATG or alemtuzumab induction immunosuppression may also improve the 1 year overall LTR survival compared to no induction.</p></div>","PeriodicalId":23304,"journal":{"name":"Transplant immunology","volume":"85 ","pages":"Article 102081"},"PeriodicalIF":1.6,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141580931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prior to ABOi liver transplant with PD-1 inhibitor in patients with hepatocellular carcinoma: A case report","authors":"Yipeng Pan ,&nbsp;Jicheng Hu ,&nbsp;Tao Li ,&nbsp;Shanbin Zhang ,&nbsp;Wanbang Zhou ,&nbsp;Jiangbo Sun ,&nbsp;Jianli Wang ,&nbsp;Wei Li ,&nbsp;Jian Xu","doi":"10.1016/j.trim.2024.102079","DOIUrl":"10.1016/j.trim.2024.102079","url":null,"abstract":"<div><h3>Background</h3><p>Liver transplantation (LT) is a unique and effective method for treating end-stage liver diseases and acute liver failure, bringing hope to many patients with liver cancer. LT is currently widely used in the treatment of liver diseases. However, there have been no patients with liver cancer who have undergone ABO-incompatible (ABOi) LT after treatment with the programmed cell death protein 1 (PD-1) inhibitor reported in the literature.</p></div><div><h3>Case presentation</h3><p>A patient with liver cancer who received sintilimab injection, an anti-PD1 therapy, before LT was admitted in the transplantation centre. This patient underwent ABOi LT. The perioperative treatment strategy of this patient was reported. A desensitisation protocol was conducted urgently for the patient before operation, and the immunosuppression programme of LT was adjusted. After operation, isoagglutinin titer and liver function indicators were strictly monitored. The patient recovered well after operation, and no sign of rejection reaction was observed.</p></div><div><h3>Conclusion</h3><p>We reported a patient with hepatocellular carcinoma (HCC) who received PD-1 inhibitor treatment before operation and successfully underwent ABOi LT. The present case report provides novel insights into the perioperative management of utilizing PD-1 inhibitors prior to ABOi LT in patients diagnosed with hepatocellular carcinoma (HCC).</p></div>","PeriodicalId":23304,"journal":{"name":"Transplant immunology","volume":"85 ","pages":"Article 102079"},"PeriodicalIF":1.6,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141535401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship between compliance with immunosuppressive therapy and religious attitudes of kidney transplant patients","authors":"Hatice Güzel ,&nbsp;Özlem Ovayolu ,&nbsp;Nimet Ovayolu ,&nbsp;Sümeyra Mihrap Ilter","doi":"10.1016/j.trim.2024.102080","DOIUrl":"10.1016/j.trim.2024.102080","url":null,"abstract":"<div><h3>Objective</h3><p>This study was conducted to examine the relationship between adherence to immunosuppressive therapy and religious attitudes of kidney transplant patients.</p></div><div><h3>Method</h3><p>The research was conducted descriptively with patients followed in the transplantation clinic of the between 2015 and 2019. The sample consisted of 142 patients who met the study criteria. Before starting the study, necessary permissions were obtained from the institution, ethics committee and patients.</p></div><div><h3>Results</h3><p>There was a significant relationship between marital status, educational status, income status and the mean score of the immunosuppressive treatment adherence scale, and between family type and the mean score of the religious attitude scale (<em>p</em> &lt; 0.05). Of these results only; It was determined that there was a significant relationship between the priority order of drugs in life, duration of renal failure and time after transplantation and drug compliance scale average score (<em>p</em> &lt; 0.05). Those who do not want to donate their kidneys to their relatives, those who do not want to donate organs when they die, those whose religious beliefs affect drug compliance, the duration of kidney failure is between 1 and 12 months and the period after transplantation 13- It was determined that those who had 60 months had a “more positive religious attitude” (<em>p</em> &lt; 0.05).</p></div><div><h3>Conclusion</h3><p>It was found that the mean score of the immunosuppressive treatment compliance scale of kidney transplant patients was at a good level, while the mean score of religious attitude was below the middle level. In addition, there was no significant relationship between the mean score of the immunosuppressive treatment compliance scale and the mean score of the religious attitude scale.</p></div>","PeriodicalId":23304,"journal":{"name":"Transplant immunology","volume":"85 ","pages":"Article 102080"},"PeriodicalIF":1.6,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141535403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The circ_0003928/miR-31-5p/MAPK6 cascade affects high glucose-induced inflammatory response, fibrosis and oxidative stress in HK-2 cells","authors":"","doi":"10.1016/j.trim.2024.102078","DOIUrl":"10.1016/j.trim.2024.102078","url":null,"abstract":"<div><h3>Background</h3><p>Diabetic nephropathy (DN) is a severe diabetic complication disorder. Circular RNAs (circRNAs) actively participate in DN pathogenesis. In this report, we sought to define a new mechanism of circ_0003928 in regulating high glucose (HG)-induced HK-2 cells.</p></div><div><h3>Methods</h3><p>To construct a DN cell model, we treated HK-2 cells with HG. Cell viability and apoptosis were detected by CCK-8 and flow cytometry, respectively. The inflammatory cytokines were quantified by ELISA. Protein analysis was performed by immunoblotting, and mRNA expression was detected by quantitative PCR. The circ_0003928/miR-31-5p and miR-31-5p/MAPK6 relationships were validated by RNA pull-down and luciferase assays.</p></div><div><h3>Results</h3><p>HG promoted HK-2 cell apoptosis, fibrosis and oxidative stress. Circ_0003928 and MAPK6 levels were enhanced and miR-31-5p level was decreased in HK-2 cells after HG treatment. Circ_0003928 disruption promoted cell growth and inhibited apoptosis, inflammatory response, fibrosis and oxidative stress in HG-induced HK-2 cells. Circ_0003928 targeted miR-31-5p, and MAPK6 was a target of miR-31-5p. Circ_0003928 regulated MAPK6 expression through miR-31-5p. The functions of circ_0003928 disruption in HG-induced HK-2 cells were reversed by miR-31-5p downregulation or MAPK6 upregulation.</p></div><div><h3>Conclusion</h3><p>Circ_0003928 exerts regulatory impacts on HG-induced apoptosis, inflammation, fibrosis and oxidative stress in human HK-2 cells by the miR-31-5p/MAPK6 axis.</p></div>","PeriodicalId":23304,"journal":{"name":"Transplant immunology","volume":"86 ","pages":"Article 102078"},"PeriodicalIF":1.6,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141535402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roles of M1 and M2 macrophage infiltration in post-renal transplant antibody-mediated rejection","authors":"Xiaoxiao Shao","doi":"10.1016/j.trim.2024.102076","DOIUrl":"10.1016/j.trim.2024.102076","url":null,"abstract":"<div><h3>Background</h3><p>We aimed to analyze the roles of M1 and M2 macrophage infiltration in post-renal transplant antibody-mediated rejection (AMR).</p></div><div><h3>Methods</h3><p>A total of 102 recipients who underwent renal allotransplant from January 2020 to February 2023 were divided into an immune tolerance group (<em>n</em> = 56) and a rejection group (<em>n</em> = 46). The transplant renal biopsy specimens were harvested by ultrasound-guided puncture. The M1 and M2 macrophages in renal tissues were counted, and the M1/M2 ratio was calculated. The numbers of M1 and M2 macrophages and M1/M2 ratios in patients with different severities of interstitial fibrosis/tubular atrophy (IF/TA) and different degrees of tubulointerstitial inflammatory cell infiltration were compared. The predictive values of M1 and M2 macrophages and M1/M2 ratio for post-renal transplant AMR were clarified.</p></div><div><h3>Results</h3><p>The rejection group had significantly more M1 and M2 macrophages and higher M1/M2 ratio than those of the immune tolerance group (<em>P &lt;</em> 0.05). In the rejection group, infiltrating macrophages were mainly distributed in the glomerular and interstitial capillaries, with M1 macrophages being the predominant type. With increasing severity of IF/TA, the numbers of M1 and M2 macrophages and M1/M2 ratio rose in patients with post-renal transplant AMR (<em>P &lt;</em> 0.05). The numbers and ratio had significant positive correlations with the levels of blood urea nitrogen and serum creatinine (<em>P &lt;</em> 0.05). The areas under the curves (AUCs) of numbers and M1 and M2 macrophages and M1/M2 ratio for predicting post-renal transplant AMR were 0.856, 0.839 and 0.887, respectively. The combined detection had AUC of 0.911 (95% CI: 0.802–0.986), sensitivity of 90.43% and specificity of 83.42%.</p></div><div><h3>Conclusions</h3><p>Significant macrophage infiltration is present in the case of post-renal transplant AMR, and closely related to the severity of IF/TA and the degree of tubulointerstitial inflammatory cell infiltration.</p></div>","PeriodicalId":23304,"journal":{"name":"Transplant immunology","volume":"85 ","pages":"Article 102076"},"PeriodicalIF":1.6,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141493573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}