血浆循环miR-638、miR-6511b-5p、miR-3613-5p、miR-455-3p、miR-5787和miR-548a-3p作为急性髓系白血病患者同种异体造血干细胞移植后免疫重建的无创生物标志物

IF 1.4 4区 医学 Q4 IMMUNOLOGY
Marzieh Izadifard , Mohammad Ahmadvand , Bahram Chahardouli , Mohammad Vaezi , Ghasem Janbabai , Ghazal Seghatoleslami , Mehran Bahrami , Marjan Yaghmaie , Maryam Barkhordar
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引用次数: 0

摘要

同种异体造血干细胞移植(Allogeneic hematopoietic stem cell transplantation, alloo - hsct)是治疗急性髓系白血病(AML)的一种可行方法,尽管它存在免疫重建延迟和造血重建失败等风险。本研究旨在探索循环miRNA水平作为移植后免疫重建的生物标志物的潜力。方法:这项观察性研究是在2020-2023年在伊朗sharati医院接受hla匹配的兄弟姐妹供体的异基因造血干细胞移植的新生非m3 AML患者中进行的。因此,采用十色多参数流式细胞术对移植前和移植后第30天采集的血液样本进行NK细胞、T细胞和B细胞的免疫表型测定。同时,使用定量逆转录聚合酶链反应(RT qPCR)定量miR-638、miR-6511b-5p、miR-3613-5p、miR-455-3p、miR-5787和miR-548a-3p的血浆水平。结果:miR-638、miR-3613-5p、miR-455-3p、miR-548a-3p的表达与CD4+ T细胞、CD4+/CD8+ T细胞比值、CD3-/16+/56-细胞、血小板计数呈正相关。升高的miR-455-3p和miR-3613-5p表达与更高的CD3-/16+/56-细胞相关(P分别 = 0.0475和P = 0.0325)。同样,miR-638上调与CD4+ T细胞(P = 0.0112)和CD4+/CD8+ T细胞比值(P = 0.006)升高相关,而miR-548a-3p上调与CD4+/CD8+ T细胞比值(P = 0.0353)和血小板计数(P = 0.0191)升高相关。相反,miR-3613-5p和miR-6511b-5p与CD8+ T细胞呈显著负相关(P = 0.03和P = 0.0246),而miR-5787与CD3+/16-/56+细胞呈负相关(P = 0.025)。结论:我们的研究结果表明,细胞亚群的分化受特定mirna的调控。此外,基于mirna的策略可能用于AML的免疫治疗。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Plasma-circulating miR-638, miR-6511b-5p, miR-3613-5p, miR-455-3p, miR-5787, and miR-548a-3p as noninvasive biomarkers of immune reconstitution post-allogeneic hematopoietic stem cell transplantation in acute myeloid leukemia patients

Introduction

Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a viable treatment option for acute myeloid leukemia (AML), though it carries risks including delayed immune reconstitution and hematopoietic reconstitution failure. This study aimed to explore the potential of circulating miRNA levels as biomarkers for post-transplant immune reconstitution.

Methods

This observational study was carried out on de novo non-M3 AML patients receiving allo-HSCT from HLA-matched sibling donors at Shariati Hospital, Iran in 2020–2023. Accordingly, the immunophenotype of NK cells, T cells, and B cells was determined by ten-color multiparameter flow cytometry on blood samples collected pre-transplantation and at day +30 post-transplantation. Concurrently, plasma levels of miR-638, miR-6511b-5p, miR-3613-5p, miR-455-3p, miR-5787, and miR-548a-3p were quantified using quantitative reverse transcription–polymerase chain reaction (RT qPCR).

Results

The expression of miR-638, miR-3613-5p, miR-455-3p, and miR-548a-3p positively correlated with CD4+ T cells, CD4+/CD8+ T cell ratio, CD3/16+/56 cells, and platelet count. Elevated miR-455-3p and miR-3613-5p expressions were associated with higher CD3/16+/56 cells (P = 0.0475 and P = 0.0325, respectively). Similarly, miR-638 upregulation correlated with increases in CD4+ T cells (P = 0.0112) and the CD4+/CD8+ T cell ratio (P = 0.006), while miR-548a-3p upregulation was associated with increases in the CD4+/CD8+ T cell ratio (P = 0.0353) and platelet count (P = 0.0191). Conversely, miR-3613-5p and miR-6511b-5p had considerable negative correlations with CD8+ T cells (P = 0.03 and P = 0.0246, respectively), whereas miR-5787 negatively correlated with CD3+/16/56+ cells (P = 0.025).

Conclusion

Our findings suggest that differentiation of cell subpopulations is regulated by specific miRNAs. Furthermore, miRNA-based strategies may be developed for immunotherapeutic treatments of AML.
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来源期刊
Transplant immunology
Transplant immunology 医学-免疫学
CiteScore
2.10
自引率
13.30%
发文量
198
审稿时长
48 days
期刊介绍: Transplant Immunology will publish up-to-date information on all aspects of the broad field it encompasses. The journal will be directed at (basic) scientists, tissue typers, transplant physicians and surgeons, and research and data on all immunological aspects of organ-, tissue- and (haematopoietic) stem cell transplantation are of potential interest to the readers of Transplant Immunology. Original papers, Review articles and Hypotheses will be considered for publication and submitted manuscripts will be rapidly peer-reviewed and published. They will be judged on the basis of scientific merit, originality, timeliness and quality.
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