Marzieh Izadifard , Mohammad Ahmadvand , Bahram Chahardouli , Mohammad Vaezi , Ghasem Janbabai , Ghazal Seghatoleslami , Mehran Bahrami , Marjan Yaghmaie , Maryam Barkhordar
{"title":"血浆循环miR-638、miR-6511b-5p、miR-3613-5p、miR-455-3p、miR-5787和miR-548a-3p作为急性髓系白血病患者同种异体造血干细胞移植后免疫重建的无创生物标志物","authors":"Marzieh Izadifard , Mohammad Ahmadvand , Bahram Chahardouli , Mohammad Vaezi , Ghasem Janbabai , Ghazal Seghatoleslami , Mehran Bahrami , Marjan Yaghmaie , Maryam Barkhordar","doi":"10.1016/j.trim.2025.102240","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><div>Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a viable treatment option for acute myeloid leukemia (AML), though it carries risks including delayed immune reconstitution and hematopoietic reconstitution failure. This study aimed to explore the potential of circulating miRNA levels as biomarkers for post-transplant immune reconstitution.</div></div><div><h3>Methods</h3><div>This observational study was carried out on de novo non-M3 AML patients receiving allo-HSCT from HLA-matched sibling donors at Shariati Hospital, Iran in 2020–2023. Accordingly, the immunophenotype of NK cells, T cells, and B cells was determined by ten-color multiparameter flow cytometry on blood samples collected pre-transplantation and at day +30 post-transplantation. Concurrently, plasma levels of miR-638, miR-6511b-5p, miR-3613-5p, miR-455-3p, miR-5787, and miR-548a-3p were quantified using quantitative reverse transcription–polymerase chain reaction (RT qPCR).</div></div><div><h3>Results</h3><div>The expression of miR-638, miR-3613-5p, miR-455-3p, and miR-548a-3p positively correlated with CD4<sup>+</sup> T cells, CD4<sup>+</sup>/CD8<sup>+</sup> T cell ratio, CD3<sup>−</sup>/16<sup>+</sup>/56<sup>−</sup> cells, and platelet count. Elevated miR-455-3p and miR-3613-5p expressions were associated with higher CD3<sup>−</sup>/16<sup>+</sup>/56<sup>−</sup> cells (<em>P</em> = 0.0475 and <em>P</em> = 0.0325, respectively). Similarly, miR-638 upregulation correlated with increases in CD4<sup>+</sup> T cells (<em>P</em> = 0.0112) and the CD4<sup>+</sup>/CD8<sup>+</sup> T cell ratio (<em>P</em> = 0.006), while miR-548a-3p upregulation was associated with increases in the CD4<sup>+</sup>/CD8<sup>+</sup> T cell ratio (<em>P</em> = 0.0353) and platelet count (<em>P</em> = 0.0191). Conversely, miR-3613-5p and miR-6511b-5p had considerable negative correlations with CD8<sup>+</sup> T cells (P = 0.03 and <em>P</em> = 0.0246, respectively), whereas miR-5787 negatively correlated with CD3<sup>+</sup>/16<sup>−</sup>/56<sup>+</sup> cells (<em>P</em> = 0.025).</div></div><div><h3>Conclusion</h3><div>Our findings suggest that differentiation of cell subpopulations is regulated by specific miRNAs. Furthermore, miRNA-based strategies may be developed for immunotherapeutic treatments of AML.</div></div>","PeriodicalId":23304,"journal":{"name":"Transplant immunology","volume":"91 ","pages":"Article 102240"},"PeriodicalIF":1.4000,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Plasma-circulating miR-638, miR-6511b-5p, miR-3613-5p, miR-455-3p, miR-5787, and miR-548a-3p as noninvasive biomarkers of immune reconstitution post-allogeneic hematopoietic stem cell transplantation in acute myeloid leukemia patients\",\"authors\":\"Marzieh Izadifard , Mohammad Ahmadvand , Bahram Chahardouli , Mohammad Vaezi , Ghasem Janbabai , Ghazal Seghatoleslami , Mehran Bahrami , Marjan Yaghmaie , Maryam Barkhordar\",\"doi\":\"10.1016/j.trim.2025.102240\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Introduction</h3><div>Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a viable treatment option for acute myeloid leukemia (AML), though it carries risks including delayed immune reconstitution and hematopoietic reconstitution failure. This study aimed to explore the potential of circulating miRNA levels as biomarkers for post-transplant immune reconstitution.</div></div><div><h3>Methods</h3><div>This observational study was carried out on de novo non-M3 AML patients receiving allo-HSCT from HLA-matched sibling donors at Shariati Hospital, Iran in 2020–2023. Accordingly, the immunophenotype of NK cells, T cells, and B cells was determined by ten-color multiparameter flow cytometry on blood samples collected pre-transplantation and at day +30 post-transplantation. Concurrently, plasma levels of miR-638, miR-6511b-5p, miR-3613-5p, miR-455-3p, miR-5787, and miR-548a-3p were quantified using quantitative reverse transcription–polymerase chain reaction (RT qPCR).</div></div><div><h3>Results</h3><div>The expression of miR-638, miR-3613-5p, miR-455-3p, and miR-548a-3p positively correlated with CD4<sup>+</sup> T cells, CD4<sup>+</sup>/CD8<sup>+</sup> T cell ratio, CD3<sup>−</sup>/16<sup>+</sup>/56<sup>−</sup> cells, and platelet count. Elevated miR-455-3p and miR-3613-5p expressions were associated with higher CD3<sup>−</sup>/16<sup>+</sup>/56<sup>−</sup> cells (<em>P</em> = 0.0475 and <em>P</em> = 0.0325, respectively). Similarly, miR-638 upregulation correlated with increases in CD4<sup>+</sup> T cells (<em>P</em> = 0.0112) and the CD4<sup>+</sup>/CD8<sup>+</sup> T cell ratio (<em>P</em> = 0.006), while miR-548a-3p upregulation was associated with increases in the CD4<sup>+</sup>/CD8<sup>+</sup> T cell ratio (<em>P</em> = 0.0353) and platelet count (<em>P</em> = 0.0191). Conversely, miR-3613-5p and miR-6511b-5p had considerable negative correlations with CD8<sup>+</sup> T cells (P = 0.03 and <em>P</em> = 0.0246, respectively), whereas miR-5787 negatively correlated with CD3<sup>+</sup>/16<sup>−</sup>/56<sup>+</sup> cells (<em>P</em> = 0.025).</div></div><div><h3>Conclusion</h3><div>Our findings suggest that differentiation of cell subpopulations is regulated by specific miRNAs. Furthermore, miRNA-based strategies may be developed for immunotherapeutic treatments of AML.</div></div>\",\"PeriodicalId\":23304,\"journal\":{\"name\":\"Transplant immunology\",\"volume\":\"91 \",\"pages\":\"Article 102240\"},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2025-05-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Transplant immunology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0966327425000681\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Transplant immunology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0966327425000681","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
Plasma-circulating miR-638, miR-6511b-5p, miR-3613-5p, miR-455-3p, miR-5787, and miR-548a-3p as noninvasive biomarkers of immune reconstitution post-allogeneic hematopoietic stem cell transplantation in acute myeloid leukemia patients
Introduction
Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a viable treatment option for acute myeloid leukemia (AML), though it carries risks including delayed immune reconstitution and hematopoietic reconstitution failure. This study aimed to explore the potential of circulating miRNA levels as biomarkers for post-transplant immune reconstitution.
Methods
This observational study was carried out on de novo non-M3 AML patients receiving allo-HSCT from HLA-matched sibling donors at Shariati Hospital, Iran in 2020–2023. Accordingly, the immunophenotype of NK cells, T cells, and B cells was determined by ten-color multiparameter flow cytometry on blood samples collected pre-transplantation and at day +30 post-transplantation. Concurrently, plasma levels of miR-638, miR-6511b-5p, miR-3613-5p, miR-455-3p, miR-5787, and miR-548a-3p were quantified using quantitative reverse transcription–polymerase chain reaction (RT qPCR).
Results
The expression of miR-638, miR-3613-5p, miR-455-3p, and miR-548a-3p positively correlated with CD4+ T cells, CD4+/CD8+ T cell ratio, CD3−/16+/56− cells, and platelet count. Elevated miR-455-3p and miR-3613-5p expressions were associated with higher CD3−/16+/56− cells (P = 0.0475 and P = 0.0325, respectively). Similarly, miR-638 upregulation correlated with increases in CD4+ T cells (P = 0.0112) and the CD4+/CD8+ T cell ratio (P = 0.006), while miR-548a-3p upregulation was associated with increases in the CD4+/CD8+ T cell ratio (P = 0.0353) and platelet count (P = 0.0191). Conversely, miR-3613-5p and miR-6511b-5p had considerable negative correlations with CD8+ T cells (P = 0.03 and P = 0.0246, respectively), whereas miR-5787 negatively correlated with CD3+/16−/56+ cells (P = 0.025).
Conclusion
Our findings suggest that differentiation of cell subpopulations is regulated by specific miRNAs. Furthermore, miRNA-based strategies may be developed for immunotherapeutic treatments of AML.
期刊介绍:
Transplant Immunology will publish up-to-date information on all aspects of the broad field it encompasses. The journal will be directed at (basic) scientists, tissue typers, transplant physicians and surgeons, and research and data on all immunological aspects of organ-, tissue- and (haematopoietic) stem cell transplantation are of potential interest to the readers of Transplant Immunology. Original papers, Review articles and Hypotheses will be considered for publication and submitted manuscripts will be rapidly peer-reviewed and published. They will be judged on the basis of scientific merit, originality, timeliness and quality.