Journal of Medical - Clinical Research & Reviews最新文献

{"title":"Kinetics of Sulforaphane Supplementation in an Immortalizedhuman Keratinocyte (HaCaT) Cell Line Exposed to UVB Irradiation","authors":"Huda Mohammed Al-barnawi","doi":"10.33425/2639-944x.1045","DOIUrl":"https://doi.org/10.33425/2639-944x.1045","url":null,"abstract":"Introduction: Exposure of skin to UVB irradiation leads to adverse effects on health including skin aging, cancer, etc. Sulforaphane is an antioxidant compound derived naturally from cruciferous vegetables and also known for its chemo-preventive properties. This research project aims to investigate the effect of sulforaphane supplementation in human keratinocyte (HaCaT) cells exposed to UVB irradiation. Methods and Results: Briefly, exposure to UVB has a negative impact in HaCaT cell viability in a manner where the higher the intensity of the exposure the greater the reduction in cell viability. In addition, it was observed that the greatest degree of sulforaphane-induced protection was when the cells were pre-treated (for 24 hrs) and 48 hrs after exposure to the highest dose of UVB irradiation (200mJ/cm2). In fact, at this time point (48 hrs), sulforaphane was found to cause an increased rate of cell proliferation observed at every concentration tested: 0.5μM (122.0%), 1.0μM (130.0%), 2.5μM (136.0%), 5.0μM (141.0%) and 10.0μM (111.0%). Conclusion: Our data suggest that sulforaphane significantly protected HaCaT cells from exposure to UVB. In addition, we have also demonstrated that when added post-exposure, sulforaphane was less effective in providing protection against UVB irradiation, in HaCaT cells, and was even cytotoxic at concentrations of 10.0μM and higher.","PeriodicalId":232854,"journal":{"name":"Journal of Medical - Clinical Research & Reviews","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124487478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incisional Endometriosis: A Clinical Case Report","authors":"Bárbara Luchi Montebeller, A. A. Alves, R. Ferreira, A. Filho","doi":"10.33425/2639-944x.1046","DOIUrl":"https://doi.org/10.33425/2639-944x.1046","url":null,"abstract":"Endometriosis results from the implantation of functional endometrial tissue, involving both glandular and stromal cells, outside the uterine cavity. Only rarely is endometriosis found at the site of an episiotomy, with the incidence rate of this form of the disease ranging from 0.01 to 0.06% of cases. The physiopathology of this form of endometriosis is believed to be related to the implantation of decidual cells secondary to postpartum lochia and is associated with obstetric surgical procedures. This case report provides a brief review of the literature and describes a clinical case involving a patient with a diagnosis of endometriosis in an episiotomy scar. A retrospective, observational study was conducted by analyzing the patient’s medical records. The clinical manifestations of a nodule suggestive of endometriosis at the site of her episiotomy scar were confirmed as those typically found in endometriosis, with the best treatment option being resection of the lesion.","PeriodicalId":232854,"journal":{"name":"Journal of Medical - Clinical Research & Reviews","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129791415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Typhoid Glomerulonephritis in a Child: A Rare Complication of Typhoid Fever","authors":"M. Beshir, D. Rosales","doi":"10.33425/2639-944X.1043","DOIUrl":"https://doi.org/10.33425/2639-944X.1043","url":null,"abstract":"","PeriodicalId":232854,"journal":{"name":"Journal of Medical - Clinical Research & Reviews","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115231579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of Weighted Exercise and Gait Training To Improve Function in the Ataxic Patient: A Case Study on a Patient with Acute Motor-Sensory Axonal Neuropathy","authors":"M. DeGraff, Holly Battsek","doi":"10.33425/2639-944x.1048","DOIUrl":"https://doi.org/10.33425/2639-944x.1048","url":null,"abstract":"Background: Acute motor-sensory axonal neuropathy (AMSAN) is a rare subtype of Guillain Barre Syndrome (GBS) accounting for 3-5% of cases. Common symptoms include impaired joint proprioception, ataxia, and weakness. Treatment includes medication and physical therapy (PT). Research is limited on PT protocols for management of this condition, specifically for treatment of ataxia. Objective: This case demonstrates the effectiveness of weighted exercise and gait training in an ataxic patient with AMSAN in an inpatient rehabilitation unit. Methods: Patient participated in a typical acute inpatient rehabilitation program. In addition, treatment incorporated 2-3lb ankle weights for proprioceptive feedback during supine, sitting, standing, and gait tasks starting week two of rehab stay. At week six ankle weights were removed for all training. Progress was tracked weekly via joint proprioception assessment, FIM scores, and assistance required for functional mobility. Results and Discussion: Patient completed eight weeks of intensive rehabilitation. Patient made significant improvements in proprioception of bilateral great toes, hips, ankles, and knees. Patient’s FIM scores progressed from dependent (0/1) to supervision level (5). This case suggests use of weighted exercise can effectively improve ataxia and functional independence in a patient with AMSAN. Using weights to improve proprioception and ataxia in populations such as multiple sclerosis has been documented however further research is needed in the GBS/ AMSAN population.","PeriodicalId":232854,"journal":{"name":"Journal of Medical - Clinical Research & Reviews","volume":"90 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117189131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lightning in a Bottle: Harnessing Bioelectrogenesis in Microbial Fuel Cells for Electrical Generation","authors":"Tanner Ash, E. Doyle, G. Ssenyonga, Cassie Kraham, S. Scott, J. Staveley, Adil Zuber","doi":"10.33425/2639-944X.1049","DOIUrl":"https://doi.org/10.33425/2639-944X.1049","url":null,"abstract":"Bioelectrogenic microorganisms offer an attractive alternative for generating electricity when traditional methodologies are impractical. Coupled with a fuel cell, these microorganisms provide a potentially auspicious source of renewable energy. A microbial fuel cell was constructed and tested in laboratory and field-based conditions. Preliminary experimentation shows promising results and demonstrates the practical utility of this renewable energy source.","PeriodicalId":232854,"journal":{"name":"Journal of Medical - Clinical Research & Reviews","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116172155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Case Series of Herpes Zoster in Children; Ultraviolet as a Trigger?","authors":"E. Arifin, Ninda Sari, S Suswardana","doi":"10.33425/2639-944X.1044","DOIUrl":"https://doi.org/10.33425/2639-944X.1044","url":null,"abstract":"Background: The condition of immunosuppression is a known condition that triggers reactivation of varicella zoster virus, to its manifestation as herpes zoster. Excessive exposure to ultraviolet is shown to decrease the immune system, even the effects can be systemic. Children often experience excessive UV exposure during play. Is excessive UV exposure a precursor to HZ in children? Case Report: We observed five cases of HZ in children aged 3-14 years; three of them are HZ ophthalmic, one HZ thoracic and one HZ brachialis. All had never received vaccine immunization and no history of intrauterine infection. Only one of them has suffered from varicella. There was no pathologic immunosuppression (HIV infection, organ transplant, immunosuppressant therapy, systemic disease) in these five children, but all children experienced intense UV exposure 3-5 days before the cutaneous lesions appeared. After treatment according to the HZ protocol, all recover without complications. Discussion: Various experimental models prove that UV exposure can suppress the immune system in some conditions: after vaccination, infection and provocation of allergic contact dermatitis in skin exposed directly or indirectly. This condition is in accordance with the state of immunosuppression that can trigger the reactivation of VVZ to HZ. The incidence of HZ is also reported to increase in the summer. All the patients we observed had intense UV exposure a few days before the emergence of HZ cutaneous lesions. Although only one patient clearly showed a history of varicella, subclinical VVZ infection may explain the reactivation of VVZ in all four cases. Based on the various evidence mentioned above, we conclude that intense UV exposure is very likely the main precipitating factor of HZ reactivation in children.","PeriodicalId":232854,"journal":{"name":"Journal of Medical - Clinical Research & Reviews","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128906920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Approach to Diagnosis and Treatment of Mild Traumatic Brain Injury","authors":"Joseph P. Leahy","doi":"10.33425/2639-944x.1042","DOIUrl":"https://doi.org/10.33425/2639-944x.1042","url":null,"abstract":"Our understanding of MTBI has evolved to more appreciate the centrifugal force, or rotational acceleration as a key damaging force. We are also coming to understand that the physiological damage is perhaps the most important aspect. This is not to diminish the importance of looking at the physical insult, and anatomical changes, but rather to appreciate that the physiological function is of course of paramount importance. Advances in imaging, especially in Diffusion Tensor Imaging helped to visualize anatomical damage in the brain. Now we have developed a novel QEEG method that is proving to be accurate and reliable. But more importantly, we now have a treatment that is demonstrably effective. Patients with recent history of MTBI, and also patients with very long histories of multiple MTBI’s were seen. Symptoms ranged the full gamut, including inability to concentrate without pain, memory problems, headaches, fatigue and pain upon exertion, emotional disturbances, etc. A single point EEG was done with each patient for 20 minutes; the data was analyzed using a unique combination of nonlinear equations producing and LCI (concussive index). Treatment was provided with Light MD’s LED light therapy unit for 20 or 40 minute sessions. Several different protocols were needed depending on the severity and how recent the MTBI was. Most patients felt noticeably better after the first treatment, and all patients were significantly improved, if not symptom free after 10 or fewer sessions. Conclusion: The consistent results of the EEG test demonstrate the need for further, larger scale study. The treatment results are the most encouraging, with most patients recovering, demonstrating the need for a larger scale study with more diverse testing included. Methods Testing was performed with a single point EEG unit from Mind Wave Mobile. This unit allowed us to gather non filtered EEG data, over a 20 minute period for each patient. The patients were supine with eyes closed in a dimly lit room with little to no noise. The EEG unit was place over the head with the contact point at FP1. A Bluetooth connection was established with a remote computer which received approximately 650 thousand data points during a 20 minute session. The data was then analyzed using matlab and a proprietary combination of 3 types of stochastic equations. (Research has shown that linear equations do not give reliable results, and preliminary research has demonstrated that nonlinear equations do.) Treatment consisted of LED light therapy using the Light MD unit. This unit allowed us to have a larger bandwidth of wavelengths, and pulse frequency modulation. We have found that certain combinations of wavelength and modulation frequency are too strong for the acute patient, and patients need to progress through a series of 4 different combination protocols in order to avoid flare ups, and allow complete healing. 20 minute sessions were used for more intensely symptomatic patients, and they were ","PeriodicalId":232854,"journal":{"name":"Journal of Medical - Clinical Research & Reviews","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129372083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}