Translational Animal Science最新文献

{"title":"Burlap and buddies: the effects of social enrichment (preweaning mixing) and object enrichment (burlap) on piglet performance, behavior, and welfare in the preweaning environment","authors":"Ashlyn Scott, Arielle Le Heiget, Reyna Stefanson, Jamie Ahloy-Dallaire, Meagan King","doi":"10.1093/tas/txae053","DOIUrl":"https://doi.org/10.1093/tas/txae053","url":null,"abstract":"Abstract When weaned in commercial operations, piglets are not only separated from their sow but also mixed with unfamiliar pigs in an unfamiliar environment with a new diet. These abrupt changes can be stressful for piglets, often having negative welfare consequences. Our objective was to study the effects of early-life preweaning socialization and object enrichment in the preweaning environment. We compared piglet performance, behavior, and welfare across six treatments that combined multi-litter group size (1 vs. 2 vs. 4 litters) and burlap (yes vs. no). We recorded piglet behavior and lesion-scored sows and piglets. Normal conforming data, expressed per experimental unit (and behavior data were averaged over time), were analyzed by ANOVA. When given the opportunity in the sow barn, piglets in multi-litter groups socialized with other litters. Burlap use (P = 0.08) was observed in < 5% of the observations but tended to increase in mixed litter groups. Cross-sucking was observed in ~12% of the observations within mixed litter groups and tended to increase with mixed group size (P = 0.08). While there was no difference in the proportion of piglets nursing (P > 0.10), piglets were less active in the single crate groups and most active in the groups of two litters mixed (P = 0.03). Additionally, observed piglet/sow biting behaviors did not differ between treatments; however, piglet-piglet biting (P = 0.07), and pen object manipulation (P = 0.07) tended to be observed more frequently in non-enriched groups. Piglet displacements were observed more often in non-enriched groups around the pen (P = 0.03) but tended to be observed more often in enriched groups at the teat (P = 0.07). Preweaning socialization and object enrichment had no impact on the average number of piglets weaned per sow or total piglet mortality (P > 0.10). However, the proportion of laid-on piglets decreased as the number of mixed litters increased (P = 0.02). The average number of lesions per piglet did not differ between treatments. Although the final lesion scores of sow teat and udder condition did not differ between treatments (P > 0.10), sow udder scores tended to worsen more in the single litters than in the mixed litters (P = 0.08). Overall, social and object enrichment allows piglets to socialize at a younger age and to redirect their attention toward an object (burlap) which does not negatively impact piglet or sow performance, or behavior, and may improve piglet welfare around weaning.","PeriodicalId":23272,"journal":{"name":"Translational Animal Science","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140724605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of poor maternal diet during gestation are detected in F2 offspring","authors":"N. Tillquist, S. Reed, A. Reiter, M. Kawaida, E. C. Lee, S. Zinn, K. Govoni","doi":"10.1093/tas/txae055","DOIUrl":"https://doi.org/10.1093/tas/txae055","url":null,"abstract":"Abstract Poor maternal nutrition of F0 ewes impairs F1 offspring growth, with minimal differences in glucose tolerance or select metabolic circulating factors, and independent of differences in residual feed intake (RFI). To determine if poor maternal nutrition in F0 ewes alters F2 offspring growth, circulating leptin, feed efficiency, or glucose tolerance, F0 ewes (n = 46) pregnant with twins were fed 100% (control), 60% (restricted), or 140% (over) of National Research Council requirements from days 30 ± 0.02 of gestation until parturition. At 16 to 19 mo of age, female F1 (n = 36) offspring were bred to generate F2 offspring [CON-F2 (n = 12 ewes; 6 rams), RES-F2 (n = 7 ewes; 13 rams), or OVER-F2 (n = 13 ewes; 9 rams) corresponding to diets of the granddam (F0)]. Lamb body weights (BW) and blood samples were collected weekly from days 0 to 28 and every 14 d until day 252 of age. Circulating leptin was measured in serum at days 0, 7, 14, 56, 210, and 252. An intravenous glucose tolerance test was performed at days 133 ± 0.28. At days 167 ± 0.33, individual daily intake was recorded over a 77-d feeding period to determine RFI. Rams were euthanized at days 285 ± 0.93, and body morphometrics, loin eye area (LEA), back fat thickness, and organ weights were collected and bone mineral density (BMD) and length were determined in the right hind leg. During gestation, OVER-F1 ewes tended to be 8.6% smaller than CON-F1 ewes (P ≤ 0.06). F2 offspring were of similar BW from birth to day 70 (P ≥ 0.20). However, from days 84 to 252, RES-F2 offspring tended to be 7.3% smaller than CON-F2 (P ≤ 0.10). Granddam diet did not influence F2 ram body morphometrics, organ or muscle weights, LEA, adipose deposition, or leg BMD (P ≥ 0.84). RES-F2 (−0.20) and CON-F2 (−0.45) rams tended to be more feed efficient than CON-F2 ewes (0.31; P ≤ 0.08). No effects of granddam diet were observed on glucose or insulin average or baseline concentrations, area under the curve, first-phase response, or ratio (P ≥ 0.52). However, CON-F2 rams (297 mg/dL ± 16.5) had a greater glucose peak compared with RES-F2 rams (239 mg/dL ± 11.2; P = 0.05). Peak insulin concentrations were not influenced by granddam diet (P = 0.75). At d 56, RES-F2 and OVER-F2 offspring had 53.5% and 61.8% less leptin compared with CON-F2 offspring, respectively (P ≤ 0.02). These data indicate that poor maternal nutrition impacts offspring growth into the second generation with minimal impacts on offspring RFI, glucose tolerance, and circulating leptin.","PeriodicalId":23272,"journal":{"name":"Translational Animal Science","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140723919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of gas flux and animal performance test duration of growing cattle in confined conditions","authors":"Juan de J Vargas, Maya Swenson, Sara Place","doi":"10.1093/tas/txae056","DOIUrl":"https://doi.org/10.1093/tas/txae056","url":null,"abstract":"Abstract Data from three experiments was analyzed to determine the number of visits and days to assess gas flux (CH4, CO2, and O2), dry matter intake (DMI), and average daily gain (ADG) from growing animals under confined conditions. In experiment 1, 213 animals (461 ± 91 kg initial body weight [BW]) were fed a backgrounding diet and evaluated for 60 d. In experiment 2, 169 steers (488 ± 37 kg initial BW) were fed a finishing diet and assessed for 70 d. In experiment 3, 64 steers (514 ± 42 kg initial BW) were fed a finishing diet and evaluated for 80 d. In each experiment, animals were placed in one pen with one Greenfeed and five SmartFeeds to collect gas flux and feed intake simultaneously. Gas flux was analyzed using data from 161 animals from the three experiments with 100 visits for 2 or more min or 3 or more min. Also, metabolic heat production (MHP) was estimated using the individual gas flux. Daily DMI was calculated as the daily feed intake corrected by the dry matter concentration. ADG was computed as the slope of the regression of the shrunk BW (96% BW) throughout each of the experimental periods. The mean gas flux and MHP were estimated for increasing or decreasing 5-visit intervals starting with the first or the last 5 visits and increasing or decreasing until the full 100-visit dataset was utilized, respectively. Intervals of DMI were estimated for increasing or decreasing 5-d intervals starting with the first or the last 5 d and increasing or decreasing until the end of the experimental period, respectively. Intervals of ADG were estimated for increasing or decreasing measurement period intervals until the end of the experimental period, respectively. Pearson and Spearman correlations were computed between the maximum visits or days and each shortened visit or day interval. The minimum number of visits and days was determined when correlations with the total visits were greater than 0.95. The results indicated that the minimum number of visits needed to quantify CO2, O2, and MHP accurately was 40, while CH4 was 60. A visitation length of 2 min or more or 3 min or more did not modify the gas flux determination. Thus, based on the average daily visitation in these experiments, gas flux data could be collected for 25 d. Additionally, the required days to determine DMI was 30, while ADG could not be assessed in a shorter than 60-d period.","PeriodicalId":23272,"journal":{"name":"Translational Animal Science","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140732085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Bacillus subtilis on in vitro ruminal fermentation and methane production","authors":"E. Sarmikasoglou, P. Sumadong, G. Dagaew, M. L. Johnson, J. Vinyard, G. Salas-Solís, M. Siregar, A. Faciola","doi":"10.1093/tas/txae054","DOIUrl":"https://doi.org/10.1093/tas/txae054","url":null,"abstract":"\u0000 The objective of this study was to evaluate the effect of a proprietary strain of a Bacillus subtilis on in vitro ruminal fermentation and methane production in batch culture serum bottles. One hundred forty-nine batch culture bottles were used in a complete randomized block design. The arrangement of treatments was a 3 × 3 × 4 factorial to evaluate the effects of inoculum, time, diet, and their respective interactions. There were 3 experimental runs total, where run was used as block. Inoculum treatments were 1.85 mg/mL of microcrystalline cellulose (CON); 10 billion B. subtilis plus microcrystalline cellulose (A1); and 60 billion B. subtilis plus microcrystalline cellulose (A2). Diet treatments were 0.50 g of early lactation diet (E, 30% starch), mid-lactation diet (M, 25% starch), or dry cow diet (D, 18% starch). The combination resulted in total of nine treatments. Each treatment had 5 replicates, two of which were used to determine nutrient degradability at 24 and 48 h after inoculation, and three were used to determine pH, ammonia nitrogen (NH3-N), volatile fatty acids (VFA), lactate, total gas, and methane production at 3, 6, 24, and 48 h after inoculation. Fixed effects of inoculum, diet, and their interaction were tested using the GLIMMIX procedure of SAS. Significance was declared at P ≤ 0.05. We observed that, compared to control, the supplementation of B. subtilis, decreased the production of acetate and propionate, while increased the production of butyrate, iso-butyrate, valerate, iso-valerate, and caproate within each respective diet. Additionally, the total methane production exhibited mixed responses depending on the diet type. Overall, the inclusion of B. subtilis under in vitro conditions shows potential to reduce ruminal methane production when supplemented with a mid-lactation diet, constituting a possible methane mitigation additive for dairy cattle diets.","PeriodicalId":23272,"journal":{"name":"Translational Animal Science","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140729708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Online feeding behavior monitoring of individual group-housed grow-finish pigs using a low-frequency RFID electronic feeding system.","authors":"Taran H Funk, Gary A Rohrer, Tami M Brown-Brandl, Brittney N Keel","doi":"10.1093/tas/txae051","DOIUrl":"10.1093/tas/txae051","url":null,"abstract":"<p><p>Early identification of animals in need of management intervention is critical to maximize animal health and welfare and minimize issues with productivity. Feeding behavior, captured by automated feeding systems, can be used to monitor the health and welfare status of individual pigs. Here, we present a framework for monitoring feeding behavior of grow-finish pigs in real time, using a low-frequency radio frequency identification (RFID) system. Using historical data, an autoregressive linear model for predicting daily time at the feeder was developed and utilized to detect anomalous decreases in feeding behavior associated with health status of the pig. A total of 2,826 pigs were individually monitored with our warning system over the entire grow-finish period, and health warnings were compared to caretaker diagnoses. The system detected 55.7% of the caretaker diagnoses, and on average these events were detected 2.8 d earlier than diagnosis by the caretaker. High numbers of potentially spurious health warnings, generated by the system, can be partly explained by the lack of a reliable and repeatable gold standard reference data set. Results from this work provide a solid basis for monitoring individual animals, but further improvements to the system are necessary for practical implementation.</p>","PeriodicalId":23272,"journal":{"name":"Translational Animal Science","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11143491/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141200703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First evaluation of the impact of a targeted subunit vaccine against bovine viral diarrhea virus in feedlot cattle","authors":"D. Bellido, E. Gumina, Gonzalo J Rodríguez Senes, Franco M Chiariotti, Maximiliano Audrito, Pedro M Sueldo, Gustavo M Sueldo, A. Wigdorovitz","doi":"10.1093/tas/txae046","DOIUrl":"https://doi.org/10.1093/tas/txae046","url":null,"abstract":"Abstract Bovine respiratory disease (BRD) is a serious health and economic problem in the beef industry, which is often associated with transportation and caused by different pathogens. In this study, we evaluated the effect of a novel subunit targeted vaccine against bovine viral diarrhea virus (BVDV) in feedlot cattle, a major viral agent of BRD. The core of this novel vaccine is the fusion of the BVDV structural glycoprotein, E2, to a single-chain antibody, APCH, together termed, APCH-E2. The APCH antibody targets the E2 antigen to the major histocompatibility type II molecule (MHC-II) present in antigen-presenting cells. To evaluate the vaccine, 2,992 animals were randomly allocated into two groups, control group (N = 1,491) and treatment group (N = 1,501). Animals of both groups received the routine sanitary plan: two doses of clostridial, respiratory, and rabies vaccines. Animals within the treatment group also received two doses of a targeted subunit vaccine against BVDV. Serum samples were taken on the day of the first inoculation (T0) and 90 d later (T90). Viral circulation was monitored using an anti-P80 ELISA (virus-specific) and immune response was evaluated by anti-E2 ELISA (detects virus and vaccine immune responses). Only animals treated for respiratory disease were considered positive cases of BRD. Results demonstrate that the control group had significantly more animals treated for BRD cases compared to the treatment group (5.9% vs. 3.7%, P = 0.02). The control group had a greater number of animals positive for anti-P80 antibodies and significantly fewer animals positive for anti-E2 antibodies compared to the treatment group (69% vs. 61% and 71% vs. 99%, respectively, P = 0.003), consistent with natural viral circulation within this group. The treatment group, conversely, had fewer animals positive for anti-P80 antibodies and a greater number of animals positive for anti-E2 antibodies, consistent with a robust vaccine-induced antibody response and a reduction of the BVDV circulation within this group. The data indicate the new subunit targeted vaccine induced greater anti-E2 antibodies and reduced the amount of BVD virus circulation within the treatment group leading to a fewer number of animals needing to be treated for BRD.","PeriodicalId":23272,"journal":{"name":"Translational Animal Science","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140734944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of ATP bioluminescence for rapid determination of cleanliness of livestock trailers after a commercial wash","authors":"Flavia G Letsch, Michael W Welch, Matthew Meyer, Grant A Hedblom, Eric Parr, D. Classen, Molly Dillard, Dustin D Boler","doi":"10.1093/tas/txae052","DOIUrl":"https://doi.org/10.1093/tas/txae052","url":null,"abstract":"Abstract Pathogens such as porcine epidemic diarrhea virus (PEDV), porcine reproductive and respiratory syndrome (PRRSV), and E. coli are known to spread by contaminated vehicles and equipment. Pork producers have adopted trailer wash policies where each trailer is washed, disinfected, and dried before it can return to a farm. Cleanliness of livestock trailers after washing is determined by visual inspection rather than any objective method. Adenosine triphosphate (ATP) bioluminescence is used in many industries to provide real-time feedback on surface cleanliness through the detection of ATP from organic sources. That same technology may provide trailer wash facilities a way of objectively characterizing a livestock trailer’s suitability to return to a farm after washing. Two ATP luminometers (3M Clean-Trace and Neogen AccuPoint) were used to estimate the correlation between ATP bioluminescence readings and aerobic bacterial plate counts (APCs) from sampled surfaces and to determine locations within a livestock trailer that can accurately estimate surface cleanliness. Five locations in livestock trailers were evaluated. Those locations included the nose access door (NAD), back door flush gate, rear side access door (RSAD), belly flush gate (BFG), and belly side access door (BSAD). There was a positive log–log association between the two luminometers (r = 0.59, P < 0.01). Every log unit increase in one unit, resulted in a 0.42 log increase (P < 0.01) in the other unit. ATP can come from bacteria, yeasts, molds, and manure. There was a poor association (r ≥ 0.10, P ≥ 0.02) between APCs and the ATP luminometers. Still, an increase in relative light units (RLUs) resulted in a corresponding increase in colony-forming units. The greatest area of surface contamination measured by APC was the NAD. RLUs were also greater in the NAD compared to the RSAD, the BFG, and the BSAD (P ≤ 0.01). Because APCs and luminometer RLUs provided similar outcomes, statistical process control charts were developed to determine control limits for RLUs. This provides real-time feedback to trailer wash workers in determining cleanliness outcomes for livestock trailers. These data suggest that ATP bioluminescence can be a reliable method to monitor cleaning effectiveness in livestock trailers. Bioluminescence is a monitoring tool that should be used in conjunction with microbial methods to monitor procedures for cleaning and disinfection.","PeriodicalId":23272,"journal":{"name":"Translational Animal Science","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140740992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the effects of benzoic acid on nursery and finishing pig growth performance","authors":"Katelyn N. Gaffield, Jenna J. Bromm, J. DeRouchey, M. Tokach, J. Woodworth, R. Goodband, Kiah M. Berg, J. D. De Jong, Courtney L. Pohlen, J. Gebhardt","doi":"10.1093/tas/txae049","DOIUrl":"https://doi.org/10.1093/tas/txae049","url":null,"abstract":"Abstract Three studies were conducted evaluating the use of benzoic acid in swine diets. In experiment 1, 350 weanling barrows (DNA 200 × 400; initially 5.9 ± 0.04 kg) were allotted to one of the five dietary treatments with 14 pens per treatment. Diets were fed in three phases: phase 1 from weaning to day 10, phase 2 from days 10 to 18, and phase 3 from days 18 to 38. Treatment 1 contained no benzoic acid throughout all three phases (weaning to day 42). Treatment 2 included 0.50% benzoic acid throughout all three phases. Treatment 3 contained 0.50% benzoic acid in phases 1 and 2, and 0.25% benzoic acid in phase 3. Treatment 4 contained 0.50% benzoic acid in phases 1 and 2, and no benzoic acid in phase 3. Treatment 5 contained 0.50% benzoic acid in phase 1, 0.25% benzoic acid in phase 2, and no benzoic acid in phase 3. For the overall period, pigs fed 0.50% in the first two phases and 0.25% benzoic acid in the final phase had greater (P < 0.05) average daily gain (average daily gain) than pigs fed no benzoic acid through all three phases, or pigs fed 0.50% in the first two phases and no benzoic acid in the final phase, with pigs fed the other treatments intermediate. Pigs fed 0.50% in the first two phases and 0.25% benzoic acid in the final phase had improved (P < 0.05) gain-to-feed ratio (G:F) compared with pigs fed no benzoic acid throughout all three phases, pigs fed 0.50% in the first two phases and no benzoic acid in the third phase, or pigs fed 0.50%, 0.25%, and no benzoic acid, respectively. For experiment 2, a 101-d trial was conducted using two groups of 1,053 finishing pigs (2,106 total pigs; PIC 337 × 1,050; initially 33.3 ± 1.9 kg). Dietary treatments were corn–soybean meal-dried distillers grains with solubles-based with the addition of none, 0.25%, or 0.50% benzoic acid. Overall, pigs fed increasing benzoic acid had a tendency for increased average daily feed intake (linear, P = 0.083) but decreased G:F (linear, P < 0.05). In experiment 3, 2,162 finishing pigs (DNA 600 × PIC 1050; initially 31.4 ± 2.2 kg) were used in a 109-d trial. Dietary treatments were formulated with or without 0.25% benzoic acid. For the overall experimental period, pigs fed benzoic acid had increased (P < 0.05) G:F. In summary, feeding benzoic acid elicits improved growth performance when fed throughout the entire nursery period while improved G:F in growing-finishing pigs was observed in one experiment, but not in the other.","PeriodicalId":23272,"journal":{"name":"Translational Animal Science","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140741317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional evaluation of Bacillus licheniformis PF9 for its potential in controlling enterotoxigenic Escherichia coli in weaned piglets","authors":"Haoxiang Xu, Joshua Gong, P. Lu, Paula Azevedo, Linyan Li, Hai Yu, Chengbo Yang","doi":"10.1093/tas/txae050","DOIUrl":"https://doi.org/10.1093/tas/txae050","url":null,"abstract":"Abstract During the bacterial selection, isolate PF9 demonstrated tolerance to low pH and high bile salt and an ability to extend the lifespan of Caenorhabditis elegans infected with enterotoxigenic Escherichia coli (ETEC; P < 0.05). Thirty-two weaned piglets susceptible to ETEC F4 were randomly allocated to four treatments as follows: 1) non-challenged negative control group (NNC; basal diet and piglets gavaged with phosphate-buffered saline), 2) negative control group (NC; basal diet and piglets challenged with ETEC F4, 3 × 107 CFU per pig), 3) positive control (PC; basal diet + 80 mg·kg−1 of avilamycin and piglets challenged with ETEC F4), and 4) probiotic candidate (PF9; control basal diet + 2.5 × 109 CFU·kg−1 diet of B. licheniformis PF9 and piglets challenged with ETEC F4). The infection of ETEC F4 decreased average daily gain and gain:feed in the NC group when compared to the NNC group (P < 0.05). The inoculation of ETEC F4 induced severe diarrhea at 3 h postinoculum (hpi), 36, 40 hpi in the NC group when compared to the NNC group (P < 0.05). The supplementation of B. licheniformis PF9 significantly relieved diarrhea severity at 3 hpi when compared to the NC group (P < 0.05). The inoculation of ETEC F4 reduced duodenal, jejunal, and ileal villus height (VH) in the NC group when compared to the NNC group. A significant (P < 0.05) decrease was detected in the duodenal VH in the PC and NNC groups. Moreover, the NNC group had a reduced relative mRNA level of Na+-glucose cotransporter 1 (SGLT1) when compared to the NC group (P < 0.05). Compared to the NC and NNC groups, the supplementation of B. licheniformis PF9 increased the relative mRNA levels of aminopeptidase N, occludin, zonula occludens-1, and SGLT1 (P < 0.05). The supplementation of B. licheniformis PF9 also significantly increased the relative mRNA level of excitatory amino acid transporter 1 when compared to the NC group (P < 0.05). Piglets supplemented with B. licheniformis PF9 showed lower relative abundance of Bacteroidetes in the colon than piglets from the NNC group (P < 0.05). The NNC group had a higher relative abundance of Firmicutes in the ileum than all the challenged piglets (P < 0.05); however, a lower relative abundance of Proteobacteria in the ileum and colon was observed in the NC group (P < 0.05). This study provides evidence that B. licheniformis PF9 has the potential to improve the gut health of piglets under challenging conditions.","PeriodicalId":23272,"journal":{"name":"Translational Animal Science","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140747229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Administering the maternal appeasing substance before slaughter to improve carcass characteristics of finishing cattle","authors":"S. Mackey, R. Cooke, A. Pickett","doi":"10.1093/tas/txae048","DOIUrl":"https://doi.org/10.1093/tas/txae048","url":null,"abstract":"Abstract Two experiments evaluated carcass characteristics of finishing steers administered the maternal bovine appeasing substance (mBAS) prior to slaughter. In Exp. 1, 954 Angus-influenced finishing steers housed in 6 original pens were used. Each original pen was split into a pair of experimental pens 14.3 d ± 3 d prior to slaughter, in a manner that number of steers and average pen body weight (BW; 636 ± 4 kg) were similar. An oiler containing mBAS (Ferappease Finish Cattle 5%; FERA Diagnostics and Biologicals; College Station, TX) was added to one of the experimental pens 7 d prior to slaughter (n = 6), whereas the other pen did not contain an oiler (CON; n = 6). The oiler delivered 120 mL of mBAS/steer during a 7-d period. Steer BW was recorded 7 d prior to and during loading (final BW) to the packing plant. No treatment effects were detected (P ≥ 0.51) for BW gain, final BW, and proportion of carcasses that graded Choice or Prime. Carcass dressing percentage was greater (P = 0.02) in mBAS compared with CON steers (65.9% vs. 64.2%; SEM = 0.5), which was not sufficient to impact hot carcass weight (HCW; P = 0.29). Incidence of dark-cutting carcasses did not differ between treatments (P = 0.23). In Exp. 2, 80 Angus-influenced finishing steers housed in 16 pens (5 steers/pen; 600 ± 4 kg of BW) were used. Pens were arranged in 4 rows of 4 pens/row, and rows were alternately assigned to receive an oiler containing mBAS (n = 8) or mineral oil (CON+; n = 8) 7 d prior to slaughter. Oilers were designed to deliver 120 mL/steer of mBAS or mineral oil during the 7-d period. Steer BW was recorded as in Exp. 1, and a blood sample was collected during exsanguination. No treatment effects were detected (P ≥ 0.20) for BW parameters, carcass marbling score, backfat thickness, Longissimus muscle area, yield grade, and proportion of carcasses that graded Choice or Prime. Carcass dressing was greater (P = 0.02) in mBAS steers compared with CON + (60.6 vs. 59.6%; SEM = 0.3) but HCW did not differ (P = 0.47) between treatments. Plasma cortisol concentration was less (P < 0.01) in mBAS steers compared with CON + (11.7 vs. 20.8 ng/mL; SEM = 1.6). Incidence of dark-cutting carcasses did not differ (P = 0.53) between treatments. In summary, mBAS administration to finishing cattle using oilers during the last 7 d on feed alleviated the adrenocortical stress response elicited by the process of slaughter, which likely resulted in increased carcass dressing.","PeriodicalId":23272,"journal":{"name":"Translational Animal Science","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140751748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}