Transgenic Research最新文献_第8页

β1,3-galactosyltransferase on chromosome 6 is essential for the formation of Lewis ^a structure on N-glycan in Oryza sativa. 6号染色体上的β1,3-半乳糖基转移酶对水稻N-聚糖上Lewis a结构的形成至关重要。

IF 3 3区生物学

Transgenic Research Pub Date : 2023-10-01 Epub Date: 2023-08-04 DOI: 10.1007/s11248-023-00360-y

Jae-Wan Jung, Seong-Ryong Kim

{"title":"β1,3-galactosyltransferase on chromosome 6 is essential for the formation of Lewis a structure on N-glycan in Oryza sativa.","authors":"Jae-Wan Jung, Seong-Ryong Kim","doi":"10.1007/s11248-023-00360-y","DOIUrl":"10.1007/s11248-023-00360-y","url":null,"abstract":"β1,3-galactose is the component of outer-chain elongation of complex N-glycans that, together with α1,4-fucose, forms Lewis a structures in plants. Previous studies have revealed that N-glycan maturation is mediated by sequential attachment of β1,3-galactose and α1,4-fucose by individual β1,3-galactosyltransferase (GalT) and α1,4-fucosyltransferase (1,4-FucT), respectively. Although GalT from several species has been studied, little information about GalT from rice is available. I therefore characterized three GalT candidate genes on different chromosomes in Oryza sativa. Seeds of rice lines that had T-DNA insertions in regions corresponding to individual putative GalT genes were obtained from a Rice Functional Genomic Express Database and plants grown until maturity. Homozygotes were selected from the next generation by genotyping PCR, and used for callus induction. Callus extracts of two independent T-DNA mutant rice which have T-DNA insertions at the same gene on chromosome 6 but in different exons showed highly reduced band intensity on a western blots using an anti-Lewis a antibody. Cell extracts and cultured media from suspension culture of the one of these mutant rice were further analysed by N-glycan profiling using matrix-associated laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF). Identified N-glycan species containing β1,3-galactose from both cell extracts and cultured media of knock-out mutant were less than 0.5% of total N-glycans while that of WT cells were 9.8% and 49.1%, respectively. This suggests that GalT located on rice chromosome 6 plays a major role in N-glycan galactosylation, and mutations within it lead to blockage of Lewis a epitope formation.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":" ","pages":"487-496"},"PeriodicalIF":3.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9925536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity of transgenes in sustainable management of insect pests. 转基因在害虫可持续管理中的多样性。

IF 3 3区生物学

Transgenic Research Pub Date : 2023-10-01 Epub Date: 2023-08-12 DOI: 10.1007/s11248-023-00362-w

V Rakesh, Vinay K Kalia, Amalendu Ghosh

{"title":"Diversity of transgenes in sustainable management of insect pests.","authors":"V Rakesh, Vinay K Kalia, Amalendu Ghosh","doi":"10.1007/s11248-023-00362-w","DOIUrl":"10.1007/s11248-023-00362-w","url":null,"abstract":"Insecticidal transgenes, when incorporated and expressed in plants, confer resistance against insects by producing several products having insecticidal properties. Protease inhibitors, lectins, amylase inhibitors, and chitinase genes are associated with the natural defenses developed by plants to counter insect attacks. Several toxin genes are also derived from spiders and scorpions for protection against insects. Bacillus thuringiensis Berliner is a microbial source of insecticidal toxins. Several methods have facilitated the large-scale production of transgenic plants. Bt-derived cry, cyt, vip, and sip genes, plant-derived genes such as lectins, protease inhibitors, and alpha-amylase inhibitors, insect cell wall-degrading enzymes like chitinase and some proteins like arcelins, plant defensins, and ribosome-inactivating proteins have been successfully utilized to impart resistance to insects. Besides, transgenic plants expressing double-stranded RNA have been developed with enhanced resistance. However, the long-term effects of transgenes on insect resistance, the environment, and human health must be thoroughly investigated before they are made available for commercial planting. In this chapter, the present status, prospects, and future scope of transgenes for insect pest management have been summarized and discussed.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":" ","pages":"351-381"},"PeriodicalIF":3.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9972851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Assessment of co-infection with BNYVV and BSCTV on resistance against Rhizomania disease in transgenic sugar beet plants. BNYVV和BSCTV联合感染对转基因甜菜植株抗根病能力的评价。

IF 3 3区生物学

Transgenic Research Pub Date : 2023-10-01 Epub Date: 2023-09-01 DOI: 10.1007/s11248-023-00364-8

Maryam Khoshnami, Bahar Zare, Hamideh Mardani-Mehrabad, Farshad Rakhshandehroo, Mohammad Amin Baghery, Mohammad Ali Malboobi

{"title":"Assessment of co-infection with BNYVV and BSCTV on resistance against Rhizomania disease in transgenic sugar beet plants.","authors":"Maryam Khoshnami, Bahar Zare, Hamideh Mardani-Mehrabad, Farshad Rakhshandehroo, Mohammad Amin Baghery, Mohammad Ali Malboobi","doi":"10.1007/s11248-023-00364-8","DOIUrl":"10.1007/s11248-023-00364-8","url":null,"abstract":"Sugar beet is an economically important crop and one of the major sources of sucrose around the world. Beet necrotic yellow vein virus (BNYVV) and Beet severe curly top virus (BSCTV) are two widespread viruses in sugar beet that cause severe damage to its performance. Previously, we have successfully produced resistance to BNYVV based on RNA silencing in sugar beet by introducing constructs carrying the viral coat-protein-encoding DNA sequence, CP21, in sense and anti-sense orientations. Yet, the RNA silencing-mediated resistance to a specific virus could be affected by other ones as a part of synergistic interactions. In this study, we assayed the specificity of the induced resistance against BNYVV in two sets of transgenic events, S3 and S6 carrying 5'-UTR with or without CP21-coding sequences, respectively. These events were subjected to viral challenges with either BNYVV, an Iranian isolate of BSCTV (BSCTV-Ir) or both. All the plants inoculated with just BSCTV-Ir displayed curly-leaf symptoms. However, partial resistance was evident in S3 events as shown by mild symptoms and reduced PCR amplification of the BSCTV-Ir coat protein encoding sequence. Based on the presented data, resistance to BNYVV was stable in almost all the transgenic plants co-infected with BSCTV-Ir, except for one event, S3-229. In general, it seems that the co-infection does not affect the resistance to BNYVV in transgenic plants. These findings demonstrated that the introduced RNA silencing-mediated resistance against BNYVV in transgenic sugar beets is specific and is not suppressed after co-infection with a heterologous virus.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":" ","pages":"475-485"},"PeriodicalIF":3.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10503044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas9-mediated multiple guide RNA-targeted mutagenesis in the potato. CRISPR/Cas9介导的马铃薯多导RNA靶向诱变。

IF 3 3区生物学

Transgenic Research Pub Date : 2023-10-01 Epub Date: 2023-06-18 DOI: 10.1007/s11248-023-00356-8

Laura Abeuova, Balnur Kali, Dilnur Tussipkan, Ainash Akhmetollayeva, Yerlan Ramankulov, Shuga Manabayeva

{"title":"CRISPR/Cas9-mediated multiple guide RNA-targeted mutagenesis in the potato.","authors":"Laura Abeuova, Balnur Kali, Dilnur Tussipkan, Ainash Akhmetollayeva, Yerlan Ramankulov, Shuga Manabayeva","doi":"10.1007/s11248-023-00356-8","DOIUrl":"10.1007/s11248-023-00356-8","url":null,"abstract":"CRISPR/Cas9 technology has become the most efficient method for genome editing in many plant species, including important industrial crops such as potatoes. This study used three target regions (T1, T2, and T3) in gbss exon I, whose sequences were first inserted into the BbsI sites in the appropriate guide RNA (gRNA) vector (pEn-Chimera, pMR203, pMR204, and pMR205), and then localized between the AtU6 promoter and the gRNA scaffold sequence. Expression vectors were constructed by introducing gRNA genes into the pMR287 (pYUCas9Plus) plasmids using the MultiSite Gateway system by attR and attL sites. The three target regions of mutant potato lines were analyzed. The use of CRISPR/Cas9-mediated multiple guide RNA-targeted mutagenesis allowed tri- or tetra-allelic mutant potato lines to be generated. Multiple nucleotide substitutions and indels within and around the three target sites caused a frameshift mutation that led to a premature stop codon, resulting in the production of gbss-knockout plants. Mutation frequencies and analysis of mutation patterns suggested that the stably transformed Cas9/multiple guide RNA expression constructs used in this study can induce targeted mutations efficiently in the potato genome. Full knockout of the gbss gene was analyzed by CAPS, Sanger sequencing and iodine staining. The present study demonstrated successful CRISPR/Cas9-mediated multiple guide RNA-targeted mutagenesis in the potato gbss gene by Agrobacterium-mediated transformation, resulting in an amylose-free phenotype.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":" ","pages":"383-397"},"PeriodicalIF":3.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9658479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of cucumber UBL5 promoter as a tool for transgene expression and genome editing in plants. 黄瓜UBL5启动子作为植物转基因表达和基因组编辑工具的评估。

IF 3 3区生物学

Transgenic Research Pub Date : 2023-10-01 Epub Date: 2023-06-23 DOI: 10.1007/s11248-023-00359-5

Kamonchanok Taway, Issariya Dachphun, Supachai Vuttipongchaikij, Anongpat Suttangkakul

{"title":"Evaluation of cucumber UBL5 promoter as a tool for transgene expression and genome editing in plants.","authors":"Kamonchanok Taway, Issariya Dachphun, Supachai Vuttipongchaikij, Anongpat Suttangkakul","doi":"10.1007/s11248-023-00359-5","DOIUrl":"10.1007/s11248-023-00359-5","url":null,"abstract":"Transgene expression and genome editing can help improve cucumber varieties to better respond to climate change. This study aimed to evaluate the applicability of the CsUBL5 promoter in transgene expression and genome editing in cucumber. The CsUBL5 promoter was cloned and analyzed to identify cis-elements that respond to abiotic signals, hormones, signal molecules, and nutrient treatments. 5' deletion constructs of the promoter were tested for their ability to drive GUS reporter expression in cucumber cotyledons, Arabidopsis seedlings, and tobacco leaves, and their response to various treatments including SA, light, drought, IAA, and GA was determined. The results showed that the CsUBL5 promoter effectively drove transgene expression in these plants, and their expressions under treatments were consistent with the predicted cis-elements, with some exceptions. Furthermore, the pCsUBL5-749 deletion construct can improve genome editing efficiency in cucumber when driving Cas9 expression. The editing efficiency of two sgRNAs targeting the ATG6 gene in cucumber was up to 4.6-fold higher using pCsUBL5-749 compared to a rice UBI promoter, although the effects of changing promoter on the editing efficiency is sgRNA specific. These findings highlight the potential utility of the CsUBL5 promoter for improving cucumber varieties through genetic engineering and genome editing. It also demonstrates the importance of modulating Cas9 expression to increase genome editing efficiency in cucumbers.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":" ","pages":"437-449"},"PeriodicalIF":3.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9668750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-expression of four penaeidins in transgenic rice seeds: an alternative strategy for substitute antibiotic agricultural products. 四种对虾毒素在转基因水稻种子中的共表达：替代抗生素农产品的替代策略。

IF 3 3区生物学

Transgenic Research Pub Date : 2023-10-01 Epub Date: 2023-08-03 DOI: 10.1007/s11248-023-00361-x

Xinyuan Song, Yu Qiao, Jian Ma, Xue Zhang, Jie Liu, Wen Xin, Shaochen Xing, Yunpeng Wang

{"title":"Co-expression of four penaeidins in transgenic rice seeds: an alternative strategy for substitute antibiotic agricultural products.","authors":"Xinyuan Song, Yu Qiao, Jian Ma, Xue Zhang, Jie Liu, Wen Xin, Shaochen Xing, Yunpeng Wang","doi":"10.1007/s11248-023-00361-x","DOIUrl":"10.1007/s11248-023-00361-x","url":null,"abstract":"The co-expression of multiple antimicrobial peptides (AMPs) in genetically modified (GM) crops can give plants a broader antibacterial spectrum and lower the pathogen risk of drug resistance. Therefore, four penaeidins (shrimp-derived AMPs) were fused and encoded in an artificial gene (PEN1234), driven by the seed-specific promoter Pzein, with the aim of co-expression in seeds of transgenic rice. The resistant rice plants, acquired via Agrobacterium-mediated transformation and glufosinate screening, were identified by PCR and the modified disk-diffusion method, and eight GM lines with high AMP content in the seeds were obtained. Among them, the PenOs017 line had the largest penaeidin content, at approximately 251-300 μg/g in seeds and 15-47 μg/g in roots and leaves. The AMPs in the seeds kept their antibacterial properties even after the seed had been boiled in hot water and could significantly inhibit the growth of methicillin-resistant Staphylococcus aureus, and AMPs in the leaves could effectively inhibit Xanthomonas oryzae pv. Oryzae. The results indicate that PenOs017 seeds containing AMPs are an ideal raw-material candidate for antibiotic-free food and feed, and may require fewer petrochemical fungicides or bactericides for disease control during cultivation than conventional rice.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":" ","pages":"463-473"},"PeriodicalIF":3.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9930423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioproduction of n-3 polyunsaturated fatty acids by nematode fatty acid desaturases and elongase in Drosophila melanogaster. 线虫脂肪酸去饱和酶和伸长酶在果蝇中生物生产n-3多不饱和脂肪酸。

IF 3 3区生物学

Transgenic Research Pub Date : 2023-10-01 Epub Date: 2023-08-24 DOI: 10.1007/s11248-023-00363-9

Mai Sato, Ryoma Ota, Satoru Kobayashi, Kimiko Yamakawa-Kobayashi, Takeshi Miura, Atsushi Ido, Yuya Ohhara

{"title":"Bioproduction of n-3 polyunsaturated fatty acids by nematode fatty acid desaturases and elongase in Drosophila melanogaster.","authors":"Mai Sato, Ryoma Ota, Satoru Kobayashi, Kimiko Yamakawa-Kobayashi, Takeshi Miura, Atsushi Ido, Yuya Ohhara","doi":"10.1007/s11248-023-00363-9","DOIUrl":"10.1007/s11248-023-00363-9","url":null,"abstract":"n-3 polyunsaturated fatty acids (n-3 PUFAs), including α-linolenic acid and eicosapentaenoic acid (EPA), are essential nutrients for vertebrates including humans. Vertebrates are n-3 PUFA-auxotrophic; hence, dietary intake of n-3 PUFAs is required for their normal physiology and development. Although fish meal and oil have been utilized as primary sources of n-3 PUFAs by humans and aquaculture, these traditional n-3 PUFA sources are expected to be exhausted because of the increasing consumption requirements of humans. Hence, it is necessary to establish alternative n-3 PUFA sources to reduce the gap between the supply and demand of n-3 PUFAs. Here, we investigated whether insects, which are considered as a novel source of essential nutrients, could store n-3 PUFAs by the forced expression of n-3 PUFA biosynthetic enzymes. We utilized Drosophila as an insect model to generate transgenic strains expressing Caenorhabditis elegans PUFA biosynthetic enzymes and examined their effects on the proportion of fatty acids. The ubiquitous expression of methyl-end desaturase FAT-1 prominently enhanced the proportions of α-linolenic acid, indicating that FAT-1 is useful for metabolic engineering to fortify α-linolenic acid in insect. Furthermore, the ubiquitous expression of nematode front-end desaturases (FAT-3 and FAT-4), PUFA elongase (ELO-1), and FAT-1 led to EPA bioproduction. Hence, nematode PUFA biosynthetic genes may serve as powerful genetic tools for enhancing the proportion of EPA in insects. This study represents the first step toward the establishment of n-3 PUFA-producing insects.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":" ","pages":"411-421"},"PeriodicalIF":3.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10063130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential of metabolomics in assessing global compositional changes resulting from the application of CRISPR/Cas9 technologies. 代谢组学在评估CRISPR/Cas9技术应用导致的全球成分变化中的潜力

IF 3 3区生物学

Transgenic Research Pub Date : 2023-08-01 DOI: 10.1007/s11248-023-00347-9

Margit Drapal, Eugenia M A Enfissi, Juliana Almeida, Elzbieta Rapacz, Marilise Nogueira, Paul D Fraser

{"title":"The potential of metabolomics in assessing global compositional changes resulting from the application of CRISPR/Cas9 technologies.","authors":"Margit Drapal, Eugenia M A Enfissi, Juliana Almeida, Elzbieta Rapacz, Marilise Nogueira, Paul D Fraser","doi":"10.1007/s11248-023-00347-9","DOIUrl":"https://doi.org/10.1007/s11248-023-00347-9","url":null,"abstract":"Exhaustive analysis of genetically modified crops over multiple decades has increased societal confidence in the technology. New Plant Breeding Techniques are now emerging with improved precision and the ability to generate products containing no foreign DNA and mimic/replicate conventionally bred varieties. In the present study, metabolomic analysis was used to compare (i) tobacco genotypes with and without the CRISPR associated protein 9 (Cas9), (ii) tobacco lines with the edited and non-edited DE-ETIOLATED-1 gene without phenotype and (iii) leaf and fruit tissue from stable non-edited tomato progeny with and without the Cas9. In all cases, multivariate analysis based on the difference test using LC-HRMS/MS and GC-MS data indicated no significant difference in their metabolomes. The variations in metabolome composition that were evident could be associated with the processes of tissue culture regeneration and/or transformation (e.g. interaction with Agrobacterium). Metabolites responsible for the variance included quantitative changes of abundant, well characterised metabolites such as phenolics (e.g. chlorogenic acid) and several common sugars such as fructose. This study provides fundamental data on the characterisation of gene edited crops, that are important for the evaluation of the technology and its assessment. The approach also suggests that metabolomics could contribute to routine product-based analysis of crops/foods generated from New Plant Breeding approaches.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"32 4","pages":"265-278"},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10322889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel approach to achieving more efficient production of the mature form of human IL-37 in plants. 一种在植物中更有效地生产成熟形式的人IL-37的新方法。

IF 3 3区生物学

Transgenic Research Pub Date : 2023-08-01 DOI: 10.1007/s11248-023-00351-z

Yao Zhang, Nadiyah Alqazlan, Zihe Meng, Jingyao Zhao, Nan Liu, Yuxin Zhang, Mingfeng Feng, Shengwu Ma, Aoxue Wang

{"title":"A novel approach to achieving more efficient production of the mature form of human IL-37 in plants.","authors":"Yao Zhang, Nadiyah Alqazlan, Zihe Meng, Jingyao Zhao, Nan Liu, Yuxin Zhang, Mingfeng Feng, Shengwu Ma, Aoxue Wang","doi":"10.1007/s11248-023-00351-z","DOIUrl":"https://doi.org/10.1007/s11248-023-00351-z","url":null,"abstract":"Interleukin-37 is a newly discovered cytokine that plays a pivotal role in suppressing innate inflammation and acquired immunity. We have recently expressed both the mature(mat-) and pro-forms of human IL-37b in plants and demonstrated that while both forms of the plant-made hIL-37b are functional, pmat-hIL37b exhibited significantly greater activity than ppro-IL-37b. Compared to ppro-hIL-37b, on the other hand, the expression level of pmat-hIL-37b was substantially lower (100.5 µg versus 1.05 µg/g fresh leaf mass or 1% versus 0.01% TSP). Since the difference between ppro-hIL-37b and pmat-hIL-37b is that ppro-hIL-37b contains a signal sequence not cleavable by plant cells, we reasoned that this signal sequence would play a key role in stabilizing the ppro-hIL-37b protein. Here, we describe a novel approach to enhancing pmat-hIL-37b production in plants based on incorporation of a gene sequence encoding tobacco etch virus (TEV) protease between the signal peptide and the mature hIL-37b, including a TEV cleavage site at the C-termini of TEV protease. The rationale is that when expressed as a sp-TEV-matIL-37b fusion protein, the stabilizing properties of the signal peptide of pro-hIL-37b will be awarded to its fusion partners, resulting in increased yield of target proteins. The fusion protein is then expected to cleave itself in vivo to yield a mature pmat-hIL-37b. Indeed, when a sp-TEV-matIL-37b fusion gene was expressed in stable-transformed plants, a prominent band corresponding to dimeric pmat-hIL-37b was detected, with expression yields reaching 42.5 µg/g fresh leaf mass in the best expression lines. Bioassays demonstrated that plant-made mature pmat-hIL-37b is functional.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"32 4","pages":"279-291"},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9961004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative safety assessment of genetically modified crops: focus on equivalence with reference varieties could contribute to more efficient and effective field trials. 转基因作物的比较安全性评估:注重与参考品种的等效性可以促进更高效和有效的田间试验。

IF 3 3区生物学

Transgenic Research Pub Date : 2023-08-01 Epub Date: 2023-05-22 DOI: 10.1007/s11248-023-00344-y

Gijs A Kleter, Hilko van der Voet, Jasper Engel, Jan-Pieter van der Berg

{"title":"Comparative safety assessment of genetically modified crops: focus on equivalence with reference varieties could contribute to more efficient and effective field trials.","authors":"Gijs A Kleter, Hilko van der Voet, Jasper Engel, Jan-Pieter van der Berg","doi":"10.1007/s11248-023-00344-y","DOIUrl":"10.1007/s11248-023-00344-y","url":null,"abstract":"The initial compositional analysis of plants plays an important role within the internationally harmonized comparative safety assessment approach for genetically modified plants. Current EFSA guidance prescribes two types of comparison, namely difference tests with regard to a conventional comparator or control, and equivalence tests with regard to a collection of commercial reference varieties. The experience gained so far shows that most of the statistically significant differences between the test and control can be discounted based on the fact that they are still within equivalence limits of reference varieties with a presumed history of safe use. Inclusion of a test variety and reference varieties into field trial design, and of the statistical equivalence test would already suffice for the purpose of finding relevant parameters that warrant further assessment, hence both the inclusion of a conventional counterpart and the performance of difference testing can be omitted. This would also allow for the inclusion of safety testing regimes into plant variety testing VCU (value for cultivation and use) or other, independent variety trials.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"32 4","pages":"235-250"},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10409827/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9967553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0