Transgenic Research最新文献_第7页

The potential of metabolomics in assessing global compositional changes resulting from the application of CRISPR/Cas9 technologies. 代谢组学在评估CRISPR/Cas9技术应用导致的全球成分变化中的潜力

IF 3 3区生物学

Transgenic Research Pub Date : 2023-08-01 DOI: 10.1007/s11248-023-00347-9

Margit Drapal, Eugenia M A Enfissi, Juliana Almeida, Elzbieta Rapacz, Marilise Nogueira, Paul D Fraser

{"title":"The potential of metabolomics in assessing global compositional changes resulting from the application of CRISPR/Cas9 technologies.","authors":"Margit Drapal, Eugenia M A Enfissi, Juliana Almeida, Elzbieta Rapacz, Marilise Nogueira, Paul D Fraser","doi":"10.1007/s11248-023-00347-9","DOIUrl":"https://doi.org/10.1007/s11248-023-00347-9","url":null,"abstract":"Exhaustive analysis of genetically modified crops over multiple decades has increased societal confidence in the technology. New Plant Breeding Techniques are now emerging with improved precision and the ability to generate products containing no foreign DNA and mimic/replicate conventionally bred varieties. In the present study, metabolomic analysis was used to compare (i) tobacco genotypes with and without the CRISPR associated protein 9 (Cas9), (ii) tobacco lines with the edited and non-edited DE-ETIOLATED-1 gene without phenotype and (iii) leaf and fruit tissue from stable non-edited tomato progeny with and without the Cas9. In all cases, multivariate analysis based on the difference test using LC-HRMS/MS and GC-MS data indicated no significant difference in their metabolomes. The variations in metabolome composition that were evident could be associated with the processes of tissue culture regeneration and/or transformation (e.g. interaction with Agrobacterium). Metabolites responsible for the variance included quantitative changes of abundant, well characterised metabolites such as phenolics (e.g. chlorogenic acid) and several common sugars such as fructose. This study provides fundamental data on the characterisation of gene edited crops, that are important for the evaluation of the technology and its assessment. The approach also suggests that metabolomics could contribute to routine product-based analysis of crops/foods generated from New Plant Breeding approaches.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10322889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel approach to achieving more efficient production of the mature form of human IL-37 in plants. 一种在植物中更有效地生产成熟形式的人IL-37的新方法。

IF 3 3区生物学

Transgenic Research Pub Date : 2023-08-01 DOI: 10.1007/s11248-023-00351-z

Yao Zhang, Nadiyah Alqazlan, Zihe Meng, Jingyao Zhao, Nan Liu, Yuxin Zhang, Mingfeng Feng, Shengwu Ma, Aoxue Wang

{"title":"A novel approach to achieving more efficient production of the mature form of human IL-37 in plants.","authors":"Yao Zhang, Nadiyah Alqazlan, Zihe Meng, Jingyao Zhao, Nan Liu, Yuxin Zhang, Mingfeng Feng, Shengwu Ma, Aoxue Wang","doi":"10.1007/s11248-023-00351-z","DOIUrl":"https://doi.org/10.1007/s11248-023-00351-z","url":null,"abstract":"Interleukin-37 is a newly discovered cytokine that plays a pivotal role in suppressing innate inflammation and acquired immunity. We have recently expressed both the mature(mat-) and pro-forms of human IL-37b in plants and demonstrated that while both forms of the plant-made hIL-37b are functional, pmat-hIL37b exhibited significantly greater activity than ppro-IL-37b. Compared to ppro-hIL-37b, on the other hand, the expression level of pmat-hIL-37b was substantially lower (100.5 µg versus 1.05 µg/g fresh leaf mass or 1% versus 0.01% TSP). Since the difference between ppro-hIL-37b and pmat-hIL-37b is that ppro-hIL-37b contains a signal sequence not cleavable by plant cells, we reasoned that this signal sequence would play a key role in stabilizing the ppro-hIL-37b protein. Here, we describe a novel approach to enhancing pmat-hIL-37b production in plants based on incorporation of a gene sequence encoding tobacco etch virus (TEV) protease between the signal peptide and the mature hIL-37b, including a TEV cleavage site at the C-termini of TEV protease. The rationale is that when expressed as a sp-TEV-matIL-37b fusion protein, the stabilizing properties of the signal peptide of pro-hIL-37b will be awarded to its fusion partners, resulting in increased yield of target proteins. The fusion protein is then expected to cleave itself in vivo to yield a mature pmat-hIL-37b. Indeed, when a sp-TEV-matIL-37b fusion gene was expressed in stable-transformed plants, a prominent band corresponding to dimeric pmat-hIL-37b was detected, with expression yields reaching 42.5 µg/g fresh leaf mass in the best expression lines. Bioassays demonstrated that plant-made mature pmat-hIL-37b is functional.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9961004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative safety assessment of genetically modified crops: focus on equivalence with reference varieties could contribute to more efficient and effective field trials. 转基因作物的比较安全性评估:注重与参考品种的等效性可以促进更高效和有效的田间试验。

IF 3 3区生物学

Transgenic Research Pub Date : 2023-08-01 Epub Date: 2023-05-22 DOI: 10.1007/s11248-023-00344-y

Gijs A Kleter, Hilko van der Voet, Jasper Engel, Jan-Pieter van der Berg

{"title":"Comparative safety assessment of genetically modified crops: focus on equivalence with reference varieties could contribute to more efficient and effective field trials.","authors":"Gijs A Kleter, Hilko van der Voet, Jasper Engel, Jan-Pieter van der Berg","doi":"10.1007/s11248-023-00344-y","DOIUrl":"10.1007/s11248-023-00344-y","url":null,"abstract":"The initial compositional analysis of plants plays an important role within the internationally harmonized comparative safety assessment approach for genetically modified plants. Current EFSA guidance prescribes two types of comparison, namely difference tests with regard to a conventional comparator or control, and equivalence tests with regard to a collection of commercial reference varieties. The experience gained so far shows that most of the statistically significant differences between the test and control can be discounted based on the fact that they are still within equivalence limits of reference varieties with a presumed history of safe use. Inclusion of a test variety and reference varieties into field trial design, and of the statistical equivalence test would already suffice for the purpose of finding relevant parameters that warrant further assessment, hence both the inclusion of a conventional counterpart and the performance of difference testing can be omitted. This would also allow for the inclusion of safety testing regimes into plant variety testing VCU (value for cultivation and use) or other, independent variety trials.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10409827/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9967553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas9-mediated knock-in of masu salmon (Oncorhyncus masou) elongase gene in the melanocortin-4 (mc4r) coding region of channel catfish (Ictalurus punctatus) genome. CRISPR/ cas9介导的渠道鲶鱼基因组黑素皮质素-4 (mc4r)编码区masu salmon (Oncorhyncus masou)伸长酶基因敲入

IF 3 3区生物学

Transgenic Research Pub Date : 2023-08-01 DOI: 10.1007/s11248-023-00346-w

Michael Coogan, De Xing, Baofeng Su, Veronica Alston, Andrew Johnson, Mohd Khan, Karim Khalil, Ahmed Elaswad, Shangjia Li, Jinhai Wang, Cuiyu Lu, Wenwen Wang, Darshika Hettiarachchi, Mei Shang, Tasnuba Hasin, Zhenkui Qin, Roger Cone, Ian A E Butts, Rex A Dunham

{"title":"CRISPR/Cas9-mediated knock-in of masu salmon (Oncorhyncus masou) elongase gene in the melanocortin-4 (mc4r) coding region of channel catfish (Ictalurus punctatus) genome.","authors":"Michael Coogan, De Xing, Baofeng Su, Veronica Alston, Andrew Johnson, Mohd Khan, Karim Khalil, Ahmed Elaswad, Shangjia Li, Jinhai Wang, Cuiyu Lu, Wenwen Wang, Darshika Hettiarachchi, Mei Shang, Tasnuba Hasin, Zhenkui Qin, Roger Cone, Ian A E Butts, Rex A Dunham","doi":"10.1007/s11248-023-00346-w","DOIUrl":"https://doi.org/10.1007/s11248-023-00346-w","url":null,"abstract":"Channel catfish, Ictalurus punctatus, have limited ability to synthesize Ω-3 fatty acids. The ccβA-msElovl2 transgene containing masu salmon, Oncorhynchus masou, elongase gene driven by the common carp, Cyprinus carpio, β-actin promoter was inserted into the channel catfish melanocortin-4 receptor (mc4r) gene site using the two-hit two-oligo with plasmid (2H2OP) method. The best performing sgRNA resulted in a knockout mutation rate of 92%, a knock-in rate of 54% and a simultaneous knockout/knock-in rate of 49%. Fish containing both the ccβA-msElovl2 transgene knock-in and mc4r knockout (Elovl2) were 41.8% larger than controls at 6 months post-hatch (p = 0.005). Mean eicosapentaenoic acid (EPA, C20:5n-3) levels in Elov2 mutants and mc4r knockout mutants (MC4R) were 121.6% and 94.1% higher than in controls, respectively (p = 0.045; p = 0.025). Observed mean docosahexaenoic acid (DHA, C22:6n-3) and total EPA + DHA content was 32.8% and 45.1% higher, respectively, in Elovl2 transgenic channel catfish than controls (p = 0.368; p = 0.025). To our knowledge this is the first example of genome engineering to simultaneously target transgenesis and knock-out a gene in a commercially important aquaculture species for multiple improved performance traits. With a high transgene integration rate, improved growth, and higher omega-3 fatty acid content, the use of Elovl2 transgenic channel catfish appears beneficial for application on commercial farms.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9971200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential oncogenic effect of tissue-specific expression of JC polyoma T antigen in digestive epithelial cells. 消化上皮细胞组织特异性表达JC多瘤T抗原的潜在致癌作用。

IF 3 3区生物学

Transgenic Research Pub Date : 2023-08-01 DOI: 10.1007/s11248-023-00352-y

Hua-Chuan Zheng, Hang Xue, Hong-Zhi Sun, Wen-Jing Yun, Zheng-Guo Cui

{"title":"The potential oncogenic effect of tissue-specific expression of JC polyoma T antigen in digestive epithelial cells.","authors":"Hua-Chuan Zheng, Hang Xue, Hong-Zhi Sun, Wen-Jing Yun, Zheng-Guo Cui","doi":"10.1007/s11248-023-00352-y","DOIUrl":"https://doi.org/10.1007/s11248-023-00352-y","url":null,"abstract":"JC polyoma virus (JCPyV), a ubiquitous polyoma virus that commonly infects people, is identified as the etiologic factor for progressive multifocal leukoencephalopathy and has been closely linked to various human cancers. Transgenic mice of CAG-loxp-Laz-loxp T antigen were established. T-antigen expression was specifically activated in gastroenterological target cells with a LacZ deletion using a cre-loxp system. Gastric poorly-differentiated carcinoma was observed in T antigen-activated mice using K19-cre (stem-like cells) and PGC-cre (chief cells), but not Atp4b-cre (parietal cells) or Capn8-cre (pit cells) mice. Spontaneous hepatocellular and colorectal cancers developed in Alb-cre (hepatocytes)/T antigen and villin-cre (intestinal cells)/T antigen transgenic mice respectively. Gastric, colorectal, and breast cancers were observed in PGC-cre/T antigen mice. Pancreatic insulinoma and ductal adenocarcinoma, gastric adenoma, and duodenal cancer were detected in Pdx1-cre/T antigen mice. Alternative splicing of T antigen mRNA occurred in all target organs of these transgenic mice. Our findings suggest that JCPyV T antigen might contribute to gastroenterological carcinogenesis with respect to cell specificity. Such spontaneous tumor models provide good tools for investigating the oncogenic roles of T antigen in cancers of the digestive system.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10409682/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10023784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

GEnZ explorer: a tool for visualizing agroclimate to inform research and regulatory risk assessment. GEnZ探索者:可视化农业气候的工具，为研究和监管风险评估提供信息。

IF 3 3区生物学

Transgenic Research Pub Date : 2023-08-01 DOI: 10.1007/s11248-023-00354-w

Rachel L Melnick, Larissa Jarvis, Paul Hendley, Monica Garcia-Alonso, Marc J Metzger, Navin Ramankutty, John L Teem, Andrew Roberts

{"title":"GEnZ explorer: a tool for visualizing agroclimate to inform research and regulatory risk assessment.","authors":"Rachel L Melnick, Larissa Jarvis, Paul Hendley, Monica Garcia-Alonso, Marc J Metzger, Navin Ramankutty, John L Teem, Andrew Roberts","doi":"10.1007/s11248-023-00354-w","DOIUrl":"https://doi.org/10.1007/s11248-023-00354-w","url":null,"abstract":"Confined field trials (CFT) of genetically engineered (GE) crops are used to generate data to inform environmental risk assessments (ERA). ERAs are required by regulatory authorities before novel GE crops can be released for cultivation. The transportability of CFT data to inform risk assessment in countries other than those where the CFT was conducted has been discussed previously in an analysis showing that the primary difference between CFT locations potentially impacting trial outcomes is the physical environment, particularly the agroclimate. This means that data from trials carried out in similar agroclimates could be considered relevant and sufficient to satisfy regulatory requirements for CFT data, irrespective of the country where the CFTs are conducted. This paper describes the development of an open-source tool to assist in determining the transportability of CFT data. This tool provides agroclimate together with overall crop production information to assist regulators and applicants in making informed choices on whether data from previous CFTs can inform an environmental risk assessment in a new country, as well as help developers determine optimal locations for planning future CFTs. The GEnZ Explorer is a freely available, thoroughly documented, and open-source tool that allows users to identify the agroclimate zones that are relevant for the production of 21 major crops and crop categories or to determine the agroclimatic zone at a specific location. This tool will help provide additional scientific justification for CFT data transportability, along with spatial visualization, to help ensure regulatory transparency.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10409678/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9967584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A DEAD box helicase Psp68 positively regulates salt stress responses in marker-free transgenic rice plants. DEAD盒解旋酶Psp68正调控无标记转基因水稻的盐胁迫反应。

IF 3 3区生物学

Transgenic Research Pub Date : 2023-08-01 DOI: 10.1007/s11248-023-00353-x

Mst Sufara Akhter Banu, Kazi Md Kamrul Huda, Md Harun-Ur-Rashid, Shahanaz Parveen, Narendra Tuteja

{"title":"A DEAD box helicase Psp68 positively regulates salt stress responses in marker-free transgenic rice plants.","authors":"Mst Sufara Akhter Banu, Kazi Md Kamrul Huda, Md Harun-Ur-Rashid, Shahanaz Parveen, Narendra Tuteja","doi":"10.1007/s11248-023-00353-x","DOIUrl":"https://doi.org/10.1007/s11248-023-00353-x","url":null,"abstract":"Helicases are the motor proteins not only involved in transcriptional and post-transcription process but also provide abiotic stress tolerance in many crops. The p68, belong to the SF2 (DEAD-box helicase) family proteins and overexpression of Psp68 providing enhanced tolerance to transgenic rice plants. In this study, salinity tolerant marker-free transgenic rice has been developed by overexpressing Psp68 gene and phenotypically characterized. The Psp68 overexpressing marker-free transgenic rice plants were initially screened in the rooting medium containing salt stress and 20% polyethylene glycol (PEG). Stable integration and overexpression of Psp68 in marker-free transgenic lines were confirmed by molecular analyses including PCR, southern, western blot, and qRT-PCR analyses. The marker-free transgenic lines showed enhanced tolerance to salinity stress as displayed by early seed germination, higher chlorophyll content, reduced necrosis, more survival rate, improved seedling growth and more grain yield per plant. Furthermore, Psp68 overexpressing marker-free transgenics also accumulated less Na+ and higher K+ ions in the presence of salinity stress. Phenotypic analyses also revealed that marker-free transgenic rice lines efficiently scavenge ROS-mediated damages as displayed by lower H2O2 and malondialdehyde content, delayed electrolyte leakage, higher photosynthetic efficiency, membrane stability, proline content and enhanced activities of antioxidants enzymes. Overall, our results confirmed that Psp68 overexpression confers salinity stress tolerance in marker-free transgenics, hence the technique could be utilized to develop genetically modified crops without any biosafety issues.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9970416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Agrobacterium-mediated in planta transformation protocol through coleoptile in rice. 农杆菌介导水稻胚芽鞘转化的研究进展。

IF 3 3区生物学

Transgenic Research Pub Date : 2023-08-01 DOI: 10.1007/s11248-023-00355-9

Un-Hyang Ho, Sam-Rang Song, Yong-Il Choe, Myong-Hyok Pak, Mi-Hyang Kim, Kang Kim, Tong-Su Ho

{"title":"Development of Agrobacterium-mediated in planta transformation protocol through coleoptile in rice.","authors":"Un-Hyang Ho, Sam-Rang Song, Yong-Il Choe, Myong-Hyok Pak, Mi-Hyang Kim, Kang Kim, Tong-Su Ho","doi":"10.1007/s11248-023-00355-9","DOIUrl":"https://doi.org/10.1007/s11248-023-00355-9","url":null,"abstract":"Genetic modification of rice is mainly carried out by Agrobacterium-mediated transformation of callus accompanied by tissue culture. It is time consuming, laborious and unapplicable for cultivars unable to induce callus. In this study, we have reported a novel gene transfer protocol that involves pulling out primary leaf from coleoptile and injection of Agrobacterium culture into the empty channel. Out of 25 plants survived after injection of Agrobacterium tumefaciens EHA105 culture harboring pCAMBIA1301-RD29A-AtDREB1A, 8 T0 plants revealed the expected size of around 811 bp corresponding to AtDREB1A gene and Southern blotting analysis on 18 T1 plants suggested introgression of AtDREB1A. 3 T2 lines (7-9, 12-3, 18-6) exhibited accumulation of free proline and soluble sugars, yet increase of chlorophyll content, but decrease of electrolyte leakage and methane dicarboxylic aldehyde under cold stress condition at the vegetative growth stage. Yield components investigation on T2 lines showed earlier heading date and no yield loss compared to wild type plants grown under normal condition. GUS expression analysis and integrated transgene detection in T0 and T1 plants followed by evaluation of cold stress tolerance in T2 lines suggest the advantage of this in planta transformation protocol to obtain transgenic rice.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9970916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of MuRF1 in Duroc pigs promotes skeletal muscle hypertrophy. 杜洛克猪MuRF1的缺失促进了骨骼肌肥大。

IF 3 3区生物学

Transgenic Research Pub Date : 2023-06-01 DOI: 10.1007/s11248-023-00342-0

Jiaping Li, Yiqing Hu, Jiajia Li, Haitao Wang, Hanyu Wu, Chengcheng Zhao, Tan Tan, Li Zhang, Di Zhu, Xu Liu, Ning Li, Xiaoxiang Hu

{"title":"Loss of MuRF1 in Duroc pigs promotes skeletal muscle hypertrophy.","authors":"Jiaping Li, Yiqing Hu, Jiajia Li, Haitao Wang, Hanyu Wu, Chengcheng Zhao, Tan Tan, Li Zhang, Di Zhu, Xu Liu, Ning Li, Xiaoxiang Hu","doi":"10.1007/s11248-023-00342-0","DOIUrl":"https://doi.org/10.1007/s11248-023-00342-0","url":null,"abstract":"Muscle mass development depends on increased protein synthesis and reduced muscle protein degradation. Muscle ring-finger protein-1 (MuRF1) plays a key role in controlling muscle atrophy. Its E3 ubiquitin ligase activity recognizes and degrades skeletal muscle proteins through the ubiquitin-proteasome system. The loss of Murf1, which encodes MuRF1, in mice leads to the accumulation of skeletal muscle proteins and alleviation of muscle atrophy. However, the function of Murf1 in agricultural animals remains unclear. Herein, we bred F1 generation Murf1+/- and F2 generation Murf1-/- Duroc pigs from F0 Murf1-/- pigs to investigate the effect of Murf1 knockout on skeletal muscle development. We found that the Murf1+/- pigs retained normal levels of muscle growth and reproduction, and their percentage of lean meat increased by 6% compared to that of the wild type (WT) pigs. Furthermore, the meat color, pH, water-holding capacity, and tenderness of the Murf1+/- pigs were similar to those of the WT pigs. The drip loss rate and intramuscular fat decreased slightly in the Murf1+/- pigs. However, the cross-sectional area of the myofibers in the longissimus dorsi increased in the adult Murf1+/- pigs. The skeletal muscle proteins MYBPC3 and actin, which are targeted by MuRF1, accumulated in the Murf1+/- and Murf1-/- pigs. Our findings show that inhibiting muscle protein degradation in MuRF1-deficient Duroc pigs increases the size of their myofibers and their percentage of lean meat without influencing their growth or pork quality. Our study demonstrates that Murf1 is a target gene for promoting skeletal muscle hypertrophy in pig breeding.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9537400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Mn-sod, PAL1, aos1 and HPL genes in soybean plants overexpressing the NmDef02 defensin. 过表达 NmDef02 防御素的大豆植株中 Mn-sod、PAL1、aos1 和 HPL 基因的表达。

IF 3 3区生物学

Transgenic Research Pub Date : 2023-06-01 Epub Date: 2023-05-02 DOI: 10.1007/s11248-023-00350-0

Alejandro E Morales, Natacha Soto, Celia Delgado, Yuniet Hernández, Leonardo Carrillo, Camilo Ferrero, Gil A Enríquez

{"title":"Expression of Mn-sod, PAL1, aos1 and HPL genes in soybean plants overexpressing the NmDef02 defensin.","authors":"Alejandro E Morales, Natacha Soto, Celia Delgado, Yuniet Hernández, Leonardo Carrillo, Camilo Ferrero, Gil A Enríquez","doi":"10.1007/s11248-023-00350-0","DOIUrl":"10.1007/s11248-023-00350-0","url":null,"abstract":"Plant defensins are a potential tool in crop improvement programs through biotechnology. Their antifungal action makes them attractive molecules for the production of transgenic plants. Information is currently lacking on what happens to the expression of defense genes in transgenic plants that overexpress a defensin. Here we show the relative expression of four defense-related genes: Mn-sod, PAL1, aos1 and HPL evaluated in two transgenic soybean events (Def1 and Def17) constitutively expressing the NmDef02 defensin gene from Nicotiana megalosiphon. The expression of these defense genes showed a differential profile in the transgenic events, with the increased expression of the aos1 gene and the repression of the Mn-sod gene in both events, when compared to the non-transgenic control. Furthermore, the expression of the PAL1 gene only increased in the Def17 event. The results indicate that although there were some changes in the expression of defense genes in transgenic plants overexpressing the defensin NmDef02; the morphoagronomic parameters evaluated were similar to the non-transgenic control. Understanding the molecular changes that occur in these transgenic plants could be of interest in the short, medium and long term.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9588586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0