Transgenic Research最新文献_第9页

CRISPR/Cas9-mediated knock-in of masu salmon (Oncorhyncus masou) elongase gene in the melanocortin-4 (mc4r) coding region of channel catfish (Ictalurus punctatus) genome. CRISPR/ cas9介导的渠道鲶鱼基因组黑素皮质素-4 (mc4r)编码区masu salmon (Oncorhyncus masou)伸长酶基因敲入

IF 3 3区生物学

Transgenic Research Pub Date : 2023-08-01 DOI: 10.1007/s11248-023-00346-w

Michael Coogan, De Xing, Baofeng Su, Veronica Alston, Andrew Johnson, Mohd Khan, Karim Khalil, Ahmed Elaswad, Shangjia Li, Jinhai Wang, Cuiyu Lu, Wenwen Wang, Darshika Hettiarachchi, Mei Shang, Tasnuba Hasin, Zhenkui Qin, Roger Cone, Ian A E Butts, Rex A Dunham

{"title":"CRISPR/Cas9-mediated knock-in of masu salmon (Oncorhyncus masou) elongase gene in the melanocortin-4 (mc4r) coding region of channel catfish (Ictalurus punctatus) genome.","authors":"Michael Coogan, De Xing, Baofeng Su, Veronica Alston, Andrew Johnson, Mohd Khan, Karim Khalil, Ahmed Elaswad, Shangjia Li, Jinhai Wang, Cuiyu Lu, Wenwen Wang, Darshika Hettiarachchi, Mei Shang, Tasnuba Hasin, Zhenkui Qin, Roger Cone, Ian A E Butts, Rex A Dunham","doi":"10.1007/s11248-023-00346-w","DOIUrl":"https://doi.org/10.1007/s11248-023-00346-w","url":null,"abstract":"Channel catfish, Ictalurus punctatus, have limited ability to synthesize Ω-3 fatty acids. The ccβA-msElovl2 transgene containing masu salmon, Oncorhynchus masou, elongase gene driven by the common carp, Cyprinus carpio, β-actin promoter was inserted into the channel catfish melanocortin-4 receptor (mc4r) gene site using the two-hit two-oligo with plasmid (2H2OP) method. The best performing sgRNA resulted in a knockout mutation rate of 92%, a knock-in rate of 54% and a simultaneous knockout/knock-in rate of 49%. Fish containing both the ccβA-msElovl2 transgene knock-in and mc4r knockout (Elovl2) were 41.8% larger than controls at 6 months post-hatch (p = 0.005). Mean eicosapentaenoic acid (EPA, C20:5n-3) levels in Elov2 mutants and mc4r knockout mutants (MC4R) were 121.6% and 94.1% higher than in controls, respectively (p = 0.045; p = 0.025). Observed mean docosahexaenoic acid (DHA, C22:6n-3) and total EPA + DHA content was 32.8% and 45.1% higher, respectively, in Elovl2 transgenic channel catfish than controls (p = 0.368; p = 0.025). To our knowledge this is the first example of genome engineering to simultaneously target transgenesis and knock-out a gene in a commercially important aquaculture species for multiple improved performance traits. With a high transgene integration rate, improved growth, and higher omega-3 fatty acid content, the use of Elovl2 transgenic channel catfish appears beneficial for application on commercial farms.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"32 4","pages":"251-264"},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9971200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential oncogenic effect of tissue-specific expression of JC polyoma T antigen in digestive epithelial cells. 消化上皮细胞组织特异性表达JC多瘤T抗原的潜在致癌作用。

IF 3 3区生物学

Transgenic Research Pub Date : 2023-08-01 DOI: 10.1007/s11248-023-00352-y

Hua-Chuan Zheng, Hang Xue, Hong-Zhi Sun, Wen-Jing Yun, Zheng-Guo Cui

{"title":"The potential oncogenic effect of tissue-specific expression of JC polyoma T antigen in digestive epithelial cells.","authors":"Hua-Chuan Zheng, Hang Xue, Hong-Zhi Sun, Wen-Jing Yun, Zheng-Guo Cui","doi":"10.1007/s11248-023-00352-y","DOIUrl":"https://doi.org/10.1007/s11248-023-00352-y","url":null,"abstract":"JC polyoma virus (JCPyV), a ubiquitous polyoma virus that commonly infects people, is identified as the etiologic factor for progressive multifocal leukoencephalopathy and has been closely linked to various human cancers. Transgenic mice of CAG-loxp-Laz-loxp T antigen were established. T-antigen expression was specifically activated in gastroenterological target cells with a LacZ deletion using a cre-loxp system. Gastric poorly-differentiated carcinoma was observed in T antigen-activated mice using K19-cre (stem-like cells) and PGC-cre (chief cells), but not Atp4b-cre (parietal cells) or Capn8-cre (pit cells) mice. Spontaneous hepatocellular and colorectal cancers developed in Alb-cre (hepatocytes)/T antigen and villin-cre (intestinal cells)/T antigen transgenic mice respectively. Gastric, colorectal, and breast cancers were observed in PGC-cre/T antigen mice. Pancreatic insulinoma and ductal adenocarcinoma, gastric adenoma, and duodenal cancer were detected in Pdx1-cre/T antigen mice. Alternative splicing of T antigen mRNA occurred in all target organs of these transgenic mice. Our findings suggest that JCPyV T antigen might contribute to gastroenterological carcinogenesis with respect to cell specificity. Such spontaneous tumor models provide good tools for investigating the oncogenic roles of T antigen in cancers of the digestive system.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"32 4","pages":"305-319"},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10409682/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10023784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

GEnZ explorer: a tool for visualizing agroclimate to inform research and regulatory risk assessment. GEnZ探索者:可视化农业气候的工具，为研究和监管风险评估提供信息。

IF 3 3区生物学

Transgenic Research Pub Date : 2023-08-01 DOI: 10.1007/s11248-023-00354-w

Rachel L Melnick, Larissa Jarvis, Paul Hendley, Monica Garcia-Alonso, Marc J Metzger, Navin Ramankutty, John L Teem, Andrew Roberts

{"title":"GEnZ explorer: a tool for visualizing agroclimate to inform research and regulatory risk assessment.","authors":"Rachel L Melnick, Larissa Jarvis, Paul Hendley, Monica Garcia-Alonso, Marc J Metzger, Navin Ramankutty, John L Teem, Andrew Roberts","doi":"10.1007/s11248-023-00354-w","DOIUrl":"https://doi.org/10.1007/s11248-023-00354-w","url":null,"abstract":"Confined field trials (CFT) of genetically engineered (GE) crops are used to generate data to inform environmental risk assessments (ERA). ERAs are required by regulatory authorities before novel GE crops can be released for cultivation. The transportability of CFT data to inform risk assessment in countries other than those where the CFT was conducted has been discussed previously in an analysis showing that the primary difference between CFT locations potentially impacting trial outcomes is the physical environment, particularly the agroclimate. This means that data from trials carried out in similar agroclimates could be considered relevant and sufficient to satisfy regulatory requirements for CFT data, irrespective of the country where the CFTs are conducted. This paper describes the development of an open-source tool to assist in determining the transportability of CFT data. This tool provides agroclimate together with overall crop production information to assist regulators and applicants in making informed choices on whether data from previous CFTs can inform an environmental risk assessment in a new country, as well as help developers determine optimal locations for planning future CFTs. The GEnZ Explorer is a freely available, thoroughly documented, and open-source tool that allows users to identify the agroclimate zones that are relevant for the production of 21 major crops and crop categories or to determine the agroclimatic zone at a specific location. This tool will help provide additional scientific justification for CFT data transportability, along with spatial visualization, to help ensure regulatory transparency.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"32 4","pages":"321-337"},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10409678/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9967584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A DEAD box helicase Psp68 positively regulates salt stress responses in marker-free transgenic rice plants. DEAD盒解旋酶Psp68正调控无标记转基因水稻的盐胁迫反应。

IF 3 3区生物学

Transgenic Research Pub Date : 2023-08-01 DOI: 10.1007/s11248-023-00353-x

Mst Sufara Akhter Banu, Kazi Md Kamrul Huda, Md Harun-Ur-Rashid, Shahanaz Parveen, Narendra Tuteja

{"title":"A DEAD box helicase Psp68 positively regulates salt stress responses in marker-free transgenic rice plants.","authors":"Mst Sufara Akhter Banu, Kazi Md Kamrul Huda, Md Harun-Ur-Rashid, Shahanaz Parveen, Narendra Tuteja","doi":"10.1007/s11248-023-00353-x","DOIUrl":"https://doi.org/10.1007/s11248-023-00353-x","url":null,"abstract":"Helicases are the motor proteins not only involved in transcriptional and post-transcription process but also provide abiotic stress tolerance in many crops. The p68, belong to the SF2 (DEAD-box helicase) family proteins and overexpression of Psp68 providing enhanced tolerance to transgenic rice plants. In this study, salinity tolerant marker-free transgenic rice has been developed by overexpressing Psp68 gene and phenotypically characterized. The Psp68 overexpressing marker-free transgenic rice plants were initially screened in the rooting medium containing salt stress and 20% polyethylene glycol (PEG). Stable integration and overexpression of Psp68 in marker-free transgenic lines were confirmed by molecular analyses including PCR, southern, western blot, and qRT-PCR analyses. The marker-free transgenic lines showed enhanced tolerance to salinity stress as displayed by early seed germination, higher chlorophyll content, reduced necrosis, more survival rate, improved seedling growth and more grain yield per plant. Furthermore, Psp68 overexpressing marker-free transgenics also accumulated less Na+ and higher K+ ions in the presence of salinity stress. Phenotypic analyses also revealed that marker-free transgenic rice lines efficiently scavenge ROS-mediated damages as displayed by lower H2O2 and malondialdehyde content, delayed electrolyte leakage, higher photosynthetic efficiency, membrane stability, proline content and enhanced activities of antioxidants enzymes. Overall, our results confirmed that Psp68 overexpression confers salinity stress tolerance in marker-free transgenics, hence the technique could be utilized to develop genetically modified crops without any biosafety issues.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"32 4","pages":"293-304"},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9970416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Agrobacterium-mediated in planta transformation protocol through coleoptile in rice. 农杆菌介导水稻胚芽鞘转化的研究进展。

IF 3 3区生物学

Transgenic Research Pub Date : 2023-08-01 DOI: 10.1007/s11248-023-00355-9

Un-Hyang Ho, Sam-Rang Song, Yong-Il Choe, Myong-Hyok Pak, Mi-Hyang Kim, Kang Kim, Tong-Su Ho

{"title":"Development of Agrobacterium-mediated in planta transformation protocol through coleoptile in rice.","authors":"Un-Hyang Ho, Sam-Rang Song, Yong-Il Choe, Myong-Hyok Pak, Mi-Hyang Kim, Kang Kim, Tong-Su Ho","doi":"10.1007/s11248-023-00355-9","DOIUrl":"https://doi.org/10.1007/s11248-023-00355-9","url":null,"abstract":"Genetic modification of rice is mainly carried out by Agrobacterium-mediated transformation of callus accompanied by tissue culture. It is time consuming, laborious and unapplicable for cultivars unable to induce callus. In this study, we have reported a novel gene transfer protocol that involves pulling out primary leaf from coleoptile and injection of Agrobacterium culture into the empty channel. Out of 25 plants survived after injection of Agrobacterium tumefaciens EHA105 culture harboring pCAMBIA1301-RD29A-AtDREB1A, 8 T0 plants revealed the expected size of around 811 bp corresponding to AtDREB1A gene and Southern blotting analysis on 18 T1 plants suggested introgression of AtDREB1A. 3 T2 lines (7-9, 12-3, 18-6) exhibited accumulation of free proline and soluble sugars, yet increase of chlorophyll content, but decrease of electrolyte leakage and methane dicarboxylic aldehyde under cold stress condition at the vegetative growth stage. Yield components investigation on T2 lines showed earlier heading date and no yield loss compared to wild type plants grown under normal condition. GUS expression analysis and integrated transgene detection in T0 and T1 plants followed by evaluation of cold stress tolerance in T2 lines suggest the advantage of this in planta transformation protocol to obtain transgenic rice.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"32 4","pages":"339-349"},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9970916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of MuRF1 in Duroc pigs promotes skeletal muscle hypertrophy. 杜洛克猪MuRF1的缺失促进了骨骼肌肥大。

IF 3 3区生物学

Transgenic Research Pub Date : 2023-06-01 DOI: 10.1007/s11248-023-00342-0

Jiaping Li, Yiqing Hu, Jiajia Li, Haitao Wang, Hanyu Wu, Chengcheng Zhao, Tan Tan, Li Zhang, Di Zhu, Xu Liu, Ning Li, Xiaoxiang Hu

{"title":"Loss of MuRF1 in Duroc pigs promotes skeletal muscle hypertrophy.","authors":"Jiaping Li, Yiqing Hu, Jiajia Li, Haitao Wang, Hanyu Wu, Chengcheng Zhao, Tan Tan, Li Zhang, Di Zhu, Xu Liu, Ning Li, Xiaoxiang Hu","doi":"10.1007/s11248-023-00342-0","DOIUrl":"https://doi.org/10.1007/s11248-023-00342-0","url":null,"abstract":"Muscle mass development depends on increased protein synthesis and reduced muscle protein degradation. Muscle ring-finger protein-1 (MuRF1) plays a key role in controlling muscle atrophy. Its E3 ubiquitin ligase activity recognizes and degrades skeletal muscle proteins through the ubiquitin-proteasome system. The loss of Murf1, which encodes MuRF1, in mice leads to the accumulation of skeletal muscle proteins and alleviation of muscle atrophy. However, the function of Murf1 in agricultural animals remains unclear. Herein, we bred F1 generation Murf1+/- and F2 generation Murf1-/- Duroc pigs from F0 Murf1-/- pigs to investigate the effect of Murf1 knockout on skeletal muscle development. We found that the Murf1+/- pigs retained normal levels of muscle growth and reproduction, and their percentage of lean meat increased by 6% compared to that of the wild type (WT) pigs. Furthermore, the meat color, pH, water-holding capacity, and tenderness of the Murf1+/- pigs were similar to those of the WT pigs. The drip loss rate and intramuscular fat decreased slightly in the Murf1+/- pigs. However, the cross-sectional area of the myofibers in the longissimus dorsi increased in the adult Murf1+/- pigs. The skeletal muscle proteins MYBPC3 and actin, which are targeted by MuRF1, accumulated in the Murf1+/- and Murf1-/- pigs. Our findings show that inhibiting muscle protein degradation in MuRF1-deficient Duroc pigs increases the size of their myofibers and their percentage of lean meat without influencing their growth or pork quality. Our study demonstrates that Murf1 is a target gene for promoting skeletal muscle hypertrophy in pig breeding.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"32 3","pages":"153-167"},"PeriodicalIF":3.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9537400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Mn-sod, PAL1, aos1 and HPL genes in soybean plants overexpressing the NmDef02 defensin. 过表达 NmDef02 防御素的大豆植株中 Mn-sod、PAL1、aos1 和 HPL 基因的表达。

IF 3 3区生物学

Transgenic Research Pub Date : 2023-06-01 Epub Date: 2023-05-02 DOI: 10.1007/s11248-023-00350-0

Alejandro E Morales, Natacha Soto, Celia Delgado, Yuniet Hernández, Leonardo Carrillo, Camilo Ferrero, Gil A Enríquez

{"title":"Expression of Mn-sod, PAL1, aos1 and HPL genes in soybean plants overexpressing the NmDef02 defensin.","authors":"Alejandro E Morales, Natacha Soto, Celia Delgado, Yuniet Hernández, Leonardo Carrillo, Camilo Ferrero, Gil A Enríquez","doi":"10.1007/s11248-023-00350-0","DOIUrl":"10.1007/s11248-023-00350-0","url":null,"abstract":"Plant defensins are a potential tool in crop improvement programs through biotechnology. Their antifungal action makes them attractive molecules for the production of transgenic plants. Information is currently lacking on what happens to the expression of defense genes in transgenic plants that overexpress a defensin. Here we show the relative expression of four defense-related genes: Mn-sod, PAL1, aos1 and HPL evaluated in two transgenic soybean events (Def1 and Def17) constitutively expressing the NmDef02 defensin gene from Nicotiana megalosiphon. The expression of these defense genes showed a differential profile in the transgenic events, with the increased expression of the aos1 gene and the repression of the Mn-sod gene in both events, when compared to the non-transgenic control. Furthermore, the expression of the PAL1 gene only increased in the Def17 event. The results indicate that although there were some changes in the expression of defense genes in transgenic plants overexpressing the defensin NmDef02; the morphoagronomic parameters evaluated were similar to the non-transgenic control. Understanding the molecular changes that occur in these transgenic plants could be of interest in the short, medium and long term.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"32 3","pages":"223-233"},"PeriodicalIF":3.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9588586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A transgenic mouse line for assaying tissue-specific changes in endoplasmic reticulum proteostasis. 用于检测组织特异性内质网蛋白稳态变化的转基因小鼠品系。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2023-06-01 Epub Date: 2023-05-03 DOI: 10.1007/s11248-023-00349-7

Reinis Svarcbahs, Sarah M Blossom, Helena S Baffoe-Bonnie, Kathleen A Trychta, Lacey K Greer, James Pickel, Mark J Henderson, Brandon K Harvey

{"title":"A transgenic mouse line for assaying tissue-specific changes in endoplasmic reticulum proteostasis.","authors":"Reinis Svarcbahs, Sarah M Blossom, Helena S Baffoe-Bonnie, Kathleen A Trychta, Lacey K Greer, James Pickel, Mark J Henderson, Brandon K Harvey","doi":"10.1007/s11248-023-00349-7","DOIUrl":"10.1007/s11248-023-00349-7","url":null,"abstract":"Maintenance of calcium homeostasis is important for proper endoplasmic reticulum (ER) function. When cellular stress conditions deplete the high concentration of calcium in the ER, ER-resident proteins are secreted into the extracellular space in a process called exodosis. Monitoring exodosis provides insight into changes in ER homeostasis and proteostasis resulting from cellular stress associated with ER calcium dysregulation. To monitor cell-type specific exodosis in the intact animal, we created a transgenic mouse line with a Gaussia luciferase (GLuc)-based, secreted ER calcium-modulated protein, SERCaMP, preceded by a LoxP-STOP-LoxP (LSL) sequence. The Cre-dependent LSL-SERCaMP mice were crossed with albumin (Alb)-Cre and dopamine transporter (DAT)-Cre mouse lines. GLuc-SERCaMP expression was characterized in mouse organs and extracellular fluids, and the secretion of GLuc-SERCaMP in response to cellular stress was monitored following pharmacological depletion of ER calcium. In LSL-SERCaMP × Alb-Cre mice, robust GLuc activity was observed only in the liver and blood, whereas in LSL-SERCaMP × DAT-Cre mice, GLuc activity was seen in midbrain dopaminergic neurons and tissue samples innervated by dopaminergic projections. After calcium depletion, we saw increased GLuc signal in the plasma and cerebrospinal fluid collected from the Alb-Cre and DAT-Cre crosses, respectively. This mouse model can be used to investigate the secretion of ER-resident proteins from specific cell and tissue types during disease pathogenesis and may aid in the identification of therapeutics and biomarkers of disease.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"32 3","pages":"209-221"},"PeriodicalIF":2.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10195735/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9580568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of Agrobacterium-mediated transformation system to Juglans sigillata Dode 'Qianhe-7'. 农杆菌介导的核桃‘千河7号’转化体系的建立

IF 3 3区生物学

Transgenic Research Pub Date : 2023-06-01 DOI: 10.1007/s11248-023-00348-8

Rong Wei, Wen'e Zhang, Chunxiang Li, Zhenkun Hao, Dong Huang, Wenlong Zhang, Xuejun Pan

{"title":"Establishment of Agrobacterium-mediated transformation system to Juglans sigillata Dode 'Qianhe-7'.","authors":"Rong Wei, Wen'e Zhang, Chunxiang Li, Zhenkun Hao, Dong Huang, Wenlong Zhang, Xuejun Pan","doi":"10.1007/s11248-023-00348-8","DOIUrl":"https://doi.org/10.1007/s11248-023-00348-8","url":null,"abstract":"An efficient genetic transformation system is of great significance for verifying gene function and improving plant breeding efficiency by gene engineering. In this study, a stable Agrobacterium mediated genetic transformation system of Juglans sigillata Dode 'Qianhe-7' was investigated using callus and negative pressure-assisted and ultrasonic-assisted transformation selection. The results showed that the axillary shoot leaves were suitable to induce callus and the callus proliferation rate could reach 516.27% when induction calli were cultured on DKW medium containing 0.5 mg L-1 indole-3-butyric acid, 1.2 mg L-1 2,4-dichlorophenoxyacetic acid and 0.5 mg L-1 kinetin for 18 d. In addition, negative pressure infection was the optimal infection method with the lowest browning rate (0.00%), high GFP conversion rate (16.67%), and better growth status. To further prove the feasibility of this genetic transformation system, the flavonol synthetase (JsFLS5) gene was successfully transformed into the into leaf-derived callus of 'Qianhe-7'. JsFLS5 expression and the content of total flavonoids in transformed callus were improved significantly compared with the untransformed callus, which proved that we had an efficient and reliable genetic transformation system using leaf-derived callus of Juglans sigillata.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"32 3","pages":"193-207"},"PeriodicalIF":3.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9535569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Efficacy of drought-tolerant and insect-protected transgenic TELA® maize traits in Nigeria. 尼日利亚抗旱防虫转基因TELA®玉米性状的效果研究

IF 3 3区生物学

Transgenic Research Pub Date : 2023-06-01 DOI: 10.1007/s11248-023-00345-x

M Oyekunle, R S Adamu, E Ndou, Y Beyene, M M Abdulmalik, S O Oikeh

{"title":"Efficacy of drought-tolerant and insect-protected transgenic TELA® maize traits in Nigeria.","authors":"M Oyekunle, R S Adamu, E Ndou, Y Beyene, M M Abdulmalik, S O Oikeh","doi":"10.1007/s11248-023-00345-x","DOIUrl":"https://doi.org/10.1007/s11248-023-00345-x","url":null,"abstract":"Assessment of efficacy of drought tolerance (DT) and insect protection (Bt) genes in maize genotypes is invaluable for commercialization and production of transgenic maize in Nigeria. Seven maize hybrids, known as TELA® maize, with stacked events of Bt insect protection (MON89034) and drought tolerance (MON87460; DroughtGard®) and their respective non-GM versions (isohybrids) developed through the TELA Maize Project were evaluated in confined field trial site at Zaria in 2020 and 2021. The objective was to assess the efficacy of stacked DT and Bt genes to seek deregulation and commercialization of both traits in Nigeria. Significant (P < 0.05-0.01) differences were observed among genotypes (G), environments (E) and genotype × environment interaction (GEI) for grain yield and most other traits under stem borer (moth species) and fall armyworm infested, drought stress, and optimum-moisture conditions, except E and GEI under drought. TELA® GM hybrids with Bt MON89034 had 19% higher yield than their non-GM isogenic versions, and 40% higher yield than the commercial checks under the target pests infestation. The foliar damage score of all the TELA® GM genotypes was ≤ 2 relative to their non-GM isogenic versions which scored ≥ 4, indicating the effectiveness of the Bt MON89034 gene in conferring resistance against stem borer and fall armyworm. Under moderate drought, pairwise comparison showed TELA® GM Hybrid 1-1 and Hybrid 2-1 had 12.4-20.4% higher (P < 0.01) yield than their isogenic versions. Under optimum-moisture condition with pests controlled, the TELA® GM and their isogenic hybrids were similar, but both had 32% higher yield than the commercial checks. Adoption of TELA® GM technology by farmers as adaptation strategy to cope with climate change, will ensure sustainability of maize production and productivity in Nigeria.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"32 3","pages":"169-178"},"PeriodicalIF":3.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9540354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0