Transgenic Research最新文献

{"title":"Expression dynamics of mCry3A and eCry3.1Ab transgenes in Bt corn hybrids across growth and environments.","authors":"Isaac Oyediran, Jared Conville, Eric Boudreau","doi":"10.1007/s11248-025-00478-1","DOIUrl":"10.1007/s11248-025-00478-1","url":null,"abstract":"<p><p>In 2017 and 2018, studies were conducted in Iowa, US across three environments with a history of greater than expected corn rootworm injury i.e., greater than one node of injury to (US EPA 2009) and one environment without rootworm injury, and at different growth stages, to determine the expression levels of mCry3A and eCry3.1Ab transgenes in the roots of different Bt corn hybrids namely the molecular stack (MZIR098), breeding stack (MIR604 × 5307), 5307 and MIR604. ELISA results showed that expressions of both mCry3A and eCry3.1Ab transgenes, were higher in the MZIR098 molecular stack and breeding stack (MIR604 × 5307), than the MIR604 and 5307 at V3 and VT growth stages over both years. To protect the roots from feeding damage by the corn rootworm larvae, expression of the transgenes must be high at the V3 growth stage. The expression of the transgene was significantly impacted by the stage of plant growth while the environments with greater than expected corn rootworm injury did not impact expression of the transgenes. It was found that the expression of mCry3A and eCry3.1Ab transgenes were high at the V3 plant growth stage compared to the VT growth stage. Stacking two or more genes together in the same plant such as the molecular and breeding stacks have the potential to protect roots in environments with higher-than-expected damage and slow down the evolution of resistance in field populations of rootworms.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":"2"},"PeriodicalIF":1.9,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145985301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploratory conversations with biodiversity-oriented civil society groups on the potential applications of gene drive-modified mosquitoes for malaria control in Tanzania.","authors":"Marceline F Finda, Maganga Sambo, Goodluck Malika, Rukiya Njalambaha, Sheikha Salum, Fredros O Okumu","doi":"10.1007/s11248-025-00476-3","DOIUrl":"10.1007/s11248-025-00476-3","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Gene drive-modified mosquitoes (GDMMs) are gaining attention as sustainable tools to complement existing malaria control strategies. Their ability to self-propagate and spread through wild mosquito populations offers the promise of low-cost, long-lasting impact, but also raises ecological, ethical, and governance concerns. In this evolving debate, civil society organizations (CSOs) are pivotal actors in shaping dialogue, representing community concerns, and influencing policy decisions. This study examined the perspectives and recommendations of biodiversity-oriented CSOs on the governance, testing, and potential application of GDMMs for malaria control in Tanzania. An exploratory qualitative design was employed, involving eight in-depth interviews, one focus group discussion, and three large group discussions with representatives from ten biodiversity-focused CSOs in Tanzania. Participants were selected purposively based on prior involvement in national or regional dialogues related to biotechnology; and the discussions focused on concerns, uncertainties and needs associated with testing and potential use of GDMMs for malaria control, as well as the balance of prospective benefits against long-term environmental risks. Transcripts were analyzed thematically using NVivo 12 Plus. Participants expressed cautious support for research on GDMMs for malaria control but raised concerns about scientific uncertainty, limited local expertise, inadequate transparency, potential transboundary effects and technological dependency. They emphasized the importance of generating robust, context-specific evidence before considering any environmental releases of gene drives; and highlighted concerns over inadequate accountability, particularly the lack of clarity on who would assume responsibility if adverse outcomes arise. They also advocated for early, inclusive, transparent, and continuous engagement with both target communities and the broader public. Lastly, to ensure objective and impartial oversight, they recommended development of local expertise that is independent of technology developers and sponsors. The CSOs' perspectives were diverse but broadly aligned with the precautionary principle, calling for preventive action amid uncertainty, clear accountability, and the pursuit of safer alternatives. Although many expressed serious reservations about gene drive mosquitoes, there was a shared recognition that research on the technology is necessary, provided it is conducted under controlled, transparent, and auditable conditions. Overall, these exploratory discussions underscored the need for: (i) balanced dialogue between advocates and skeptics, (ii) robust ethical and regulatory frameworks covering the full life cycle of the technology, (iii) sustained community and stakeholder engagement from the early stages of research and development, (iv) enhancements of in-country capacity, and (v) national sovereignty in decision-making regarding GDMMs. Demonst","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":"1"},"PeriodicalIF":1.9,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12795890/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145960495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A CRISPR/Cas9 assisted strategy for the conditional expression of human NF-kappaB c-Rel cDNA in mouse T cells: design, prospects, and challenges.","authors":"Corynn N Appolonia, Joshua T Centore, Sadhanshu Shukla, Julia Hluck, Ronald A Conlon, Parameswaran Ramakrishnan","doi":"10.1007/s11248-025-00470-9","DOIUrl":"10.1007/s11248-025-00470-9","url":null,"abstract":"<p><p>Nuclear factor-κB protein c-Rel is a critical regulator of autoimmune diabetes. We found that c-Rel O-GlcNAcylation at serine-350 increases with hyperglycemia, which results in increased transcription of proautoimmune Th1 cytokines, interleukin-2 (IL-2) and interferon-gamma (IFN-γ), and decreased transcription of the T regulatory cell transcription factor forkhead box 3 (FOXP3). To further study the translational relevance of c-Rel S350 O-GlcNAcylation in autoimmune diabetes, we sought to generate transgenic non-obese diabetic (NOD) mice conditionally expressing wildtype or mutant S350A human c-Rel cDNA in T cells downstream of the endogenous mouse REL promoter. We used CRISPR-Cas9 gene editing to insert a unique designer cassette containing floxed mouse c-Rel cDNA-STOP sequence to maintain whole body c-Rel expression, followed by a linker and human c-Rel cDNA-STOP sequence. Using comprehensive PCR analyses and high-throughput sequencing, we confirmed successful insertion of the cassette at the mouse REL locus and the expected deletion of the mouse c-Rel cDNA specifically in T cells following CD4-Cre mating. Additional characterization revealed that the knock-in transgenic mice lacked endogenous mouse c-Rel, further confirming desired interference with its natural start codon. Unexpectedly, these mice lacked mouse and human c-Rel protein expression from inserted cDNAs, which mechanistically correlated with increased CpG methylation of the c-Rel promoter region. Thus, our study presents a unique, universal molecular design and method for the generation of conditional knock-in transgenic mice expressing human genes at the endogenous mouse promoter. It also reveals a potential locus-specific challenge that may arise during the development of such novel transgenic mouse models.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"34 1","pages":"56"},"PeriodicalIF":1.9,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145775710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Agrobacterium-mediated genetic transformation: a gateway for efficient CRISPR/Cas9 gene editing in leguminous.","authors":"Imane Sarroukh, Mohammed Ibriz, Lamia Yakkou, Nezha Lebkiri, Mohamed Fokar, Driss Iraqi, Fatima Gaboun, Ghizlane Diria, Rabha Abdelwahd","doi":"10.1007/s11248-025-00468-3","DOIUrl":"10.1007/s11248-025-00468-3","url":null,"abstract":"<p><p>Climate change enhances the damaging consequences of abiotic and biotic stressors, leading to severe soil fertility loss and ecosystem degradation worldwide. Leguminous have contributed significantly to replenishing soil nitrogen via symbiotic nitrogen fixation, contributing approximately 15% of nitrogen input, which is crucial for soil health and enhancing crop production. There is an increasing integration of new biotechnological interventions, such as genome editing, including the CRISPR/Cas9 system, and transgenesis, in addition to classical breeding, to make agriculture more resilient. In this review, we examine several elements that influence the genetic transformation system employing Agrobacterium tumefaciens strains in leguminous to make it an ideal vehicle for CRISPR/Cas9 component delivery. The variables investigated in our study included the incubation period, co-cultivation duration, bacterial density, selectable marker, concentration, and growth regulators used. In addition, the selection and efficiency of the explant choice for transformation should be considered in future studies. However, there have been parallel recommendations for the gradual application of selectable markers such as kanamycin.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"34 1","pages":"57"},"PeriodicalIF":1.9,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145775677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reaumuria trigyna transcription factor RtWRKY49 confers cold tolerance to Arabidopsis thaliana.","authors":"Chao Du, Ziqi Gao","doi":"10.1007/s11248-025-00477-2","DOIUrl":"10.1007/s11248-025-00477-2","url":null,"abstract":"<p><p>Cold stress can greatly narrow the development and living spaces of plants. As one endangered xerophytic shrub, Reaumuria trigyna can adapt the harsh cold, but the underlying molecular mechanisms remain unknown. Here, the RtWRKY49 gene of Reaumuria trigyna was screened and encoded a nuclear WRKY transcription protein. RtWRKY49 expression mainly located in stem of Reaumuria trigyna, activated by cold, salt and ABA treatments but inhibited by heat and drought treatments. The overexpression of RtWRKY49 remarkably improved cold tolerance in Arabidopsis with the increased growth, chlorophyll content and decreased levels of malondialdehyde, oxygen free radical and hydrogen peroxide. Under cold stress, RtWRKY49 transgenic Arabidopsis had greater antioxidase activities (SOD, POD, CAT, and DHAR), AsA, and proline levels than wild-type plants, with a significant upregulation of AtCAT1, AtPOD, AtDHAR2, and AtP5CS1 genes. Our findings indicated that the RtWRKY49 protein improved the cold tolerance of plants by coordinating the antioxidant and osmoregulation pathways to maintain plant homeostasis under cold stress. In addition to offering the RtWRKY49 as a promising target gene in breeding cold stress resistant crop, our work also provides a basis for the research of Reaumuria trigyna response to cold stress.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"34 1","pages":"55"},"PeriodicalIF":1.9,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145775698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue culture optimization and genome editing for yield improvement of an Indian rice landrace Chittimuthyalu.","authors":"Faisal Yousuf, Manish Solanki, Surya Satyanarayana Singh, Suvarna Rani Ch, C N Neeraja, R M Sundaram, Satendra Kumar Mangrauthia","doi":"10.1007/s11248-025-00474-5","DOIUrl":"10.1007/s11248-025-00474-5","url":null,"abstract":"<p><p>Chittimuthyalu, a rice landrace from Southern India, is known for its pleasant aroma, rich nutritive value, and excellent cooking qualities. However, it has a poor plant type (tall and weak stem prone to lodging) and is low yielding. The efforts to improve such valuable rice accessions with existing cross-breeding or random mutagenesis often result in undesirable traits due to linkage drag or untargeted mutations in large numbers. Genome editing, the most precise breeding tool, offers a viable solution to address such issues. In this study, we developed an efficient tissue culture protocol for callus induction, transformation, and regeneration of Chittimuthyalu. The highest callus induction frequency was achieved on L3 basal media enriched with 2.5 mg/l 2,4-Dichlorophenoxyacetic acid (2,4-D) and 600 mg/l of both proline and glutamine. For regeneration, a combination of Thidiazuron (TDZ), 6-Benzylaminopurine (BAP), and kinetin yielded an optimal regeneration frequency. The optimized tissue culture protocol was utilized to transform a multiplex gene editing construct developed by combining the four guide RNAs designed from yield and disease resistance-associated genes OsDEP1, OsTB1, OsCKX2, and OsSWEET14. The OsDEP1genome-edited rice plants exhibit thicker culm, enhanced grain size, ~ 100% increase in the thousand-grain weight, and ~ 50% increase in total grain yield per plant. The optimized tissue culture protocol and development of further edits in the remaining genes will pave the way for improving the agronomic traits of Chittimuthyalu. This study also highlights much-needed efforts to develop efficient tissue culture and genome editing methods for wild rice species and landraces, which will help bring these hardy, climate-resilient, and nutrient-rich accessions into mainstream cultivation.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"34 1","pages":"54"},"PeriodicalIF":1.9,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145744666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional divergence of zebrafish keap1 paralogs revealed by CRISPR/Cas9-mediated gene editing: a specialized role for keap1b in inflammation.","authors":"Vu Thanh Nguyen, Bui Thi Thu Van, Tran Ngoc Uyen, Nguyen Xuan Tong, Thanh Luu Pham, Nguyen Hoang Thuy Vy, Dinh Thi Thuy, Nguyen Phuong Thuy, Makoto Kobayashi","doi":"10.1007/s11248-025-00475-4","DOIUrl":"10.1007/s11248-025-00475-4","url":null,"abstract":"<p><p>The Keap1/Nrf2 signaling pathway is a master regulator of cellular defense against oxidative and electrophilic stress. In teleosts like zebrafish (Danio rerio), whole-genome duplication resulted in two keap1 paralogs, keap1a and keap1b, whose functional specificities remain incompletely understood. This study investigates the divergent roles of these paralogs by comparing the responses of established keap1a and novel keap1b knockout larvae to distinct chemical stressors. By comparing the responses of keap1b^dl40, keap1a^dl07, and nfe2l2a^dl703 (Nrf2a) larvae to these stressors, we uncovered a striking functional dichotomy. While loss of either paralog conferred resistance to H₂O₂-induced oxidative stress, keap1b^dl40 larvae, unlike their keap1a^dl07 counterparts, exhibited extreme sensitivity to the lethal effects of CuSO₄ exposure, with survival rates plummeting to ~ 25%. This heightened sensitivity to copper sulfate was associated with a blunted transcriptional response of inflammatory markers tnf-a and c3a, suggesting that Keap1b is critical for modulating the Nrf2a-mediated response to inflammatory stress in orchestrating a viable inflammatory response. This work clarifies the non-redundant, vital function of Keap1b in the response to heavy metal-induced stress and provides a valuable genetic resource (keap1b^dl40 null allele) for future studies.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"34 1","pages":"53"},"PeriodicalIF":1.9,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145701882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the cucumber ATG6 and its promoter to enhance abiotic stress tolerance and drive stress-responsive transgene expression in plants.","authors":"Issariya Dachphun, Phasanat Supphokha, Supachai Vuttipongchaikij, Anongpat Suttangkakul","doi":"10.1007/s11248-025-00472-7","DOIUrl":"10.1007/s11248-025-00472-7","url":null,"abstract":"<p><p>Developing climate-resilient plants with enhanced tolerance to abiotic stresses such as drought and salinity is essential for mitigating the impact of climate change on agriculture. One promising approach is the heterologous expression of stress-responsive genes under appropriate regulatory elements. In this study, we evaluated the potential of the CsATG6 gene from cucumber (Cucumis sativus) to improve abiotic stress tolerance through heterologous expression and examined its promoter's utility for driving transgene expression in dicots. Overexpression of CsATG6 in Arabidopsis enhanced seedling root growth under nitrogen deficiency, drought, and salt stress, and improved survival rates following high-salinity treatment, indicating its potential application in stress-tolerant crop development. The CsATG6 promoter was cloned and analyzed for cis-acting elements responsive to environmental stimuli, hormones, and developmental signals. A series of 5' deletion constructs were fused to a GUS reporter and tested in Arabidopsis, tobacco leaves, and cassava calli under various treatments, including salicylic acid (SA), abscisic acid (ABA), drought, salt, and nitrogen starvation. Among the tested constructs, pCsATG6::GUS-1027 and pCsATG6::GUS-512 showed strong and inducible expression in response to ABA, SA, and multiple abiotic stresses. Furthermore, an ABA-repressive element within the promoter was identified and functionally validated via site-directed mutagenesis. Together, these findings demonstrate that CsATG6 is a valuable candidate gene for enhancing stress tolerance through heterologous expression, and that its promoter fragments can serve as stress- and hormone-responsive regulatory elements for transgene expression in some dicots, including economically important crops like cassava.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"34 1","pages":"52"},"PeriodicalIF":1.9,"publicationDate":"2025-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145669857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Avenues of genome editing for color trait improvement in ornamental fishes: current status and future perspectives.","authors":"S S Priyanka, Gowhar Iqbal, N C Nidarshan, Kriti Kumari, Sheetal Vanjre, Kiran Rasal, Arvind Sonwane, Manoj Brahmane, Mukunda Goswami","doi":"10.1007/s11248-025-00473-6","DOIUrl":"10.1007/s11248-025-00473-6","url":null,"abstract":"<p><p>Ornamental fish industries are growing sectors contributing significantly to livelihood, trade and export, driven by the worldwide demand for colourful and unique species. Pigmentation is the focal point of the visual appeal of ornamental fish, market value, and species-specific interaction. The pigment cells are called chromatophores, derived from neural crest cells and controlled by sophisticated genetic mechanisms, conferring these fish with distinctive colours and patterns. Historically, selective breeding and dietary pigment supplementation have been applied to enhance colouration. Such traditional practices, however, are prone to disadvantages such as slow development, genetic ambiguity, and unforeseen consequences. With the invention of genome editing, such as CRISPR-Cas9, researchers now have a sensitive and powerful tool to control pigmentation traits at the genetic level. Central pigmentation gene manipulation, such as Tyr, Mc1r, and Slc45a2, can allow researchers to introduce stable and uniform color changes. Such enhancements confer unparalleled control of fish colour, a promising avenue in the ornamental fish industry. This review discusses the genetic nature of fish pigmentation. It reports recent advances in CRISPR-based modifications and describes their possible applications and implications for future ornamental fish breeding.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"34 1","pages":"51"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145649466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel tamoxifen-inducible Mct8-CreERT2 mouse model for targeted studies of Mct8-expressing cells and thyroid hormone transport and function.","authors":"Anna Molenaar, Noémi Mallet, Marin Bralo, Luciano J Hoeher, Sonja C Schriever, Ekta Pathak, Miriam Bernecker, Timo D Müller, Ali Ertürk, Alberto Cebrian-Serrano, Paul T Pfluger","doi":"10.1007/s11248-025-00471-8","DOIUrl":"https://doi.org/10.1007/s11248-025-00471-8","url":null,"abstract":"<p><p>Deficiency of the Monocarboxylate Transporter 8 (MCT8) severely impairs thyroid hormone (TH) transport into the brain, disrupting brain development as well as peripheral TH homeostasis. Studies assessing MCT8 expression patterns and tissue-specific pathologies induced by local TH-deficiency are often inconclusive due to unreliable antibody staining and the lack of functional tools to specifically target MCT8-expressing cells. For this purpose, we generated non-inducible Mct8-Cre and tamoxifen-inducible Mct8-CreERT2 mice. Mct8-Cre;Sun1-sfGFP mice demonstrated ubiquitous Sun1-sfGFP expression, due to early recombination driven by Mct8 gene expression at the stage of trophoblast implantation. Tamoxifen injection in 6-week-old Mct8-CreERT2 mice induced reporter expression specifically in Mct8-expressing cells in the brain and peripherally in liver, kidney, and thyroid, without leaky reporter expression in vehicle controls. Using vDISCO tissue clearing and 3D-imaging of GFP-nanobody-boosted mice, we further identified the sublingual salivary gland and the prostate as prominent Mct8-expressing organs. Nuclei from Mct8-expressing cells in the brain could selectively be enriched using fluorescence-activated nuclei sorting on Mct8-CreERT2;Sun1-sfGFP mice and characterized as choroid plexus cells and tanycytes. Our new inducible Mct8-CreERT2 line provides researchers with a tool to reliably mark, enrich, and characterize Mct8-expressing cells and to genetically modify genes specifically in these cells to study thyroid hormone transport and function.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"34 1","pages":"50"},"PeriodicalIF":2.0,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12660349/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145640366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}