Transgenic Research最新文献

{"title":"Overexpression of wheat Wcor80 confers tolerance to low temperature in transgenic plant.","authors":"Luyao Wang, Jiaang Cao, Xuesong Wang, Junbao Zhang, Yushu Chen, Sen Yang, Qingyi Shao, Mengdi Yu, Zhongmin Jin, Lijie Liu","doi":"10.1007/s11248-026-00489-6","DOIUrl":"10.1007/s11248-026-00489-6","url":null,"abstract":"<p><p>Low temperature is a common abiotic stress, which can seriously restrict the normal growth and development of crops. In this study, wheat cold-regulated gene 80 (Wcor80) was isolated from \"Dongnongdongmai 1\" (Dn1) material and characterized. The expression of Wcor80 was induced by brassinolide (BR) and low temperature (LT) treatments, and it was preferentially expressed in the leaves under abscisic acid (ABA) treatment and in the tillering nodes under BR treatment. Overexpression of Wcor80 in transgenic Arabidopsis significantly increased tolerance to LT treatment. Overall, our findings suggest that Wcor80 functions as a positive regulator of resistance to LT stress, providing a theoretical basis for the molecular breeding and cultivation regulation of winter wheat.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147487242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The ladder-shape melting temperature isothermal amplification methods for detection of CaMV 35S and CP4 EPSPS genes in soybean foods.","authors":"Wei Yao, Juan Tian, Dan Zhou, Shanlu Guo, Yajie Wang, Jinxin Liu, Chunmei Song, Xiaohua Zhang, Deguo Wang, Yongzhen Wang, Yao Wang, Bailing Yin","doi":"10.1007/s11248-025-00464-7","DOIUrl":"10.1007/s11248-025-00464-7","url":null,"abstract":"<p><p>Soybean has become the most widely grown genetically modified (GM) crop in the world and it is often used to produce food and feed. With the rapid development of GM technology, there is growing controversy over the safety of GM foods. It is important to develop the methods for detecting GM products rapidly and conveniently. In this paper, the LMTIA method was combined with the Proofman probes for detection of soybean, CaMV 35S soybean and CP4 EPSPS soybean. It was shown that the optimal reaction temperatures for soybean, CaMV 35S soybean and CP4 EPSPS soybean were 59, 61 and 59 °C, respectively, and the sensitivities were correspondingly 10, 100 and 100 pg/µL. The nucleic acid amplification can be completed in 20 min. For the nine non-GM soybean foods tested, neither the Proofman-LMTIA method nor the digital PCR had detected the CaMV 35S and CP4 EPSPS genes, which were consistent with the label claims. The LMTIA method was established for the rapid detection of transgenic soybeans.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147356552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted expression of Glycine max isoflavone synthase enhances daidzein and genistein content in soybean.","authors":"Nandakumar Vidya, Krishnagowdu Saravanan, Jayachandran Halka, Kumaresan Kowsalya, Packiaraj Gurusaravanan, Muthukrishnan Anand, Gomathi Selvavinayagam, Jesudass Joseph Sahayarayan, Muthukrishnan Arun","doi":"10.1007/s11248-026-00485-w","DOIUrl":"10.1007/s11248-026-00485-w","url":null,"abstract":"<p><p>Isoflavonoids are key secondary metabolites in leguminous plants that play essential roles in plant physiology and provide significant health benefits to humans. In the isoflavone biosynthetic pathway, isoflavone synthase (IFS) catalyzes the conversion of naringenin and liquiritigenin into the bioactive isoflavones genistein and daidzein. This study aimed to enhance genistein and daidzein accumulation in soybean seeds through genetic engineering. Agrobacterium tumefaciens strain EHA105 harboring the binary vector pCAMBIA1301 containing GmIFS under the control of a seed-specific promoter (Gmβ-conglycinin) was used to transform modified half-seed explants of soybean cv. JS335. Hygromycin-B-resistant plants were regenerated, hardened, and confirmed by histochemical GUS assay. Molecular analysis by PCR validated the presence of the GmIFS transgene, yielding a 700 bp amplicon. Biochemical analysis revealed that seeds of T₀ transgenic plants showed a 1.53-fold increase in total phenolic content and a 3.67-fold increase in flavonoid content compared to non-transformed controls. Antioxidant assays demonstrated significantly higher DPPH radical-scavenging activity and ferric-reducing antioxidant power (FRAP) in GmIFS-overexpressing plants. HPLC analysis further indicated that transgenic seeds accumulated, on average, 4.07-fold higher daidzein and 1.75-fold higher genistein levels relative to control plants. qRT-PCR analysis showed significantly elevated GmIFS expression in immature cotyledons, mature cotyledons, and seeds of transgenic plants. Overall, these results demonstrate that GmIFS overexpression effectively enhances isoflavone production in soybean seeds, highlighting the potential of metabolic engineering of biosynthetic pathway genes to improve nutritional quality.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147310382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boosting β-carotene in rice and wheat grains through seed-specific expression of a modified wheat or gene.","authors":"Parul Sirohi, Ritika Vishnoi, Suchi Baliyan, Bidya Bhushan Gupta, Pratibha Demiwal, Hugo Germain, Debabrata Sircar, Harsh Chauhan","doi":"10.1007/s11248-026-00486-9","DOIUrl":"10.1007/s11248-026-00486-9","url":null,"abstract":"<p><p>Vitamin A deficiency is a major public health problem affecting up to 50% of the world's population, as staple food crops like wheat and rice, which are often poor in many essential micronutrients such as vitamin A, are major staple food crops. Biofortification of cereal crops with β-carotene (provitamin A) through genetic engineering is a potential solution to overcome vitamin A deficiency. The Orange (Or) protein is involved in the regulation of carotenoid accumulation and previous studies demonstrated high carotenoid accumulation due to a single-nucleotide polymorphism (SNP) in the CDS leading to substitution of Arg to His in the OR protein results in carotenoid accumulation. In the present study, we showed that this substitution of a single amino acid at position 110 (Arg to His) of wild-type wheat TaOr (referred to as TaOr^His110) increased β-carotene accumulation in transgenic wheat and rice plants overexpressing TaOr^His110 under the control of the seed-specific promoter Glu1D1. HPLC analysis revealed increase in β-carotene content in rice grain up to eightfold in case of TP309 (japonica) cultivar, 13-fold in case of IET10364 (indica) cultivar and sevenfold in wheat cv. CPAN1676. Additionally, most of the carotenoid biosynthetic pathway genes were found to be upregulated in TaOr^His110 overexpressing seeds of TP309 and IET10364, which positively correlates with maximum increase in β-carotene content.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147272007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The emerging impact of CRISPR and gene editing on global crop improvement.","authors":"M Mansi, Priyanka Danai","doi":"10.1007/s11248-026-00484-x","DOIUrl":"10.1007/s11248-026-00484-x","url":null,"abstract":"<p><p>The advent of CRISPR-based genome editing has revolutionized crop improvement, offering unprecedented precision and efficiency in modifying key agronomic traits. This review comprehensively examines the mechanisms, applications, and future potential of CRISPR technology in enhancing global crop production. CRISPR-Cas systems, originally identified as adaptive immune mechanisms in bacteria and archaea, have been repurposed for targeted genome editing in plants. The CRISPR-Cas9 system, in particular, has emerged as a powerful tool for introducing site-specific double-strand breaks, enabling precise genetic modifications. The three-stage process of adaptation, expression, and interference underlies the CRISPR mechanism, with guide RNAs directing Cas endonucleases to specific genomic loci. Advances in CRISPR technology have expanded its applications beyond gene knockouts, encompassing base editing, prime editing, and epigenome editing. These innovations have facilitated the development of crops with enhanced yield, stress tolerance, disease resistance, nutritional content, and post-harvest quality. However, challenges related to off-target effects, regulatory hurdles, ethical concerns, and public acceptance must be addressed to fully harness the potential of CRISPR in agriculture. Integration of CRISPR with other cutting-edge technologies, such as synthetic biology, artificial intelligence, and high-throughput phenotyping, holds immense promise for accelerating crop improvement efforts. As research continues to refine CRISPR tools and expand their applicability across diverse plant species, this transformative technology is poised to play a pivotal role in shaping a sustainable, resilient, and productive global food system for future generations.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":"8"},"PeriodicalIF":1.9,"publicationDate":"2026-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146195578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of compact CRISPR/Cas nucleases for citrus genome editing.","authors":"Yulong Zhu, Jingyu Zhang, Yunqiang Ruan, Tiangang Lei, Shunxin Li, Li Cao, Xiuping Zou, Yongrui He, Qiang Li, Shanchun Chen, Aihong Peng","doi":"10.1007/s11248-026-00481-0","DOIUrl":"10.1007/s11248-026-00481-0","url":null,"abstract":"<p><p>Gene editing technology continues to advance, and the range of available editing tools is steadily expanding. Recently, several compact and ultracompact systems have been developed, gaining considerable attention because their components can be efficiently packaged into viral vectors. To identify compact tools suitable for efficient genome editing in citrus, Casπ, CoCas9, along with their respective single guide RNAs, were synthesized, and CRISPR/Casπ and CRISPR/CoCas9 constructs were designed to assess their editing efficiency in 'Wanjincheng' orange (Citrus sinensis Osbeck). The Casπ was able to mediate genome editing in the citrus genome, although with low efficiency. In comparison, CoCas9 showed a transformation efficiency three times higher than that of the widely used SpCas9. Moreover, while the gene editing efficiency of CoCas9 was comparable to that of SpCas9, the significantly elevated transformation efficiency resulted in a significantly higher overall editing efficiency for CoCas9 relative to SpCas9. Mutation profiles generated by CoCas9 and SpCas9 were highly similar, and both nucleases displayed comparable target specificity at three potential off-target sites. These results indicate that Casπ is not suitable for application in citrus genome editing, whereas CoCas9 represents a promising alternative to SpCas9 for efficient and precise genome modification in citrus.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":"7"},"PeriodicalIF":1.9,"publicationDate":"2026-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146150682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of functional genetics tools for a model tetraploid Capsella bursa-pastoris, with focus on homoeolog-aware gene editing.","authors":"D O Omelchenko, A M Barkovskaya, L V Omelchenko, A V Klepikova, A A Penin, M D Logacheva","doi":"10.1007/s11248-026-00482-z","DOIUrl":"10.1007/s11248-026-00482-z","url":null,"abstract":"<p><p>Capsella bursa-pastoris is a recent allotetraploid and a promising model for studying early consequences of polyploidy. One of the intriguing questions in polyploid research is how new functions arise from initially identical or nearly identical homoeologous genes. Functional genetics tools, including genetic editing, can help to understand this process, but they have not been developed for C. bursa-pastoris yet. We present here the results of our study aimed at filling this gap. In particular, we compared the efficiency of floral dip transformation in six accessions of C. bursa-pastoris representing distant populations. The Asian clade accession PGL0025 had the highest efficiency of transformation (~ 1.1%). Comparison of Agrobacterium tumefaciens strains EHA105 and GV3101 (pMP90) showed that the latter is more effective. Also, we created a genome-wide gRNA database for all pairs of homoeologs of the PGL0001 accession of C. bursa-pastoris and integrated it into publicly available genome browser: https://t2e.online/igv_capsella_bursa-pastoris/ . We assessed the possibility of differential editing for two pairs of homoeologous genes with high sequence similarity (> 90%) both in vitro and in silico. Despite the test results that indicated off-target activity, we have succeeded in obtaining lines of plants with homozygous frameshift mutations in each of the homoeologs separately in vivo. We expect that these findings and resources will promote the use of C. bursa-pastoris as a model in functional genetics experiments, in particular, the studies of the fate of duplicated gene after polyploidization event.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":"6"},"PeriodicalIF":1.9,"publicationDate":"2026-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146132414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging trends in genome editing of wild animals.","authors":"Torill Blix, Anne Ingeborg Myhr","doi":"10.1007/s11248-026-00483-y","DOIUrl":"10.1007/s11248-026-00483-y","url":null,"abstract":"<p><p>Globally, nearly one million species are currently threatened with extinction, highlighting the need for more efficient solutions to biological conservation. Genome editing, which allows for faster and more precise changes in genomes, is a promising technique for boosting populations through facilitated adaptation, management of invasive or pathogenic populations, and potentially even facilitating the revival of extinct species. These approaches belong to a new field of research termed conservation biotechnology, which places a great responsibility on researchers and decision makers to ensure sustainability. In this paper, we have mapped the emerging trends in genome editing of wild animals. Current projects primarily focus on population control and de-extinction, with fewer initiatives aimed at preserving threatened species. We then explore four critical dimensions of conservation biotechnology: the technology itself, new perspectives on conservation practices, research organization, and governance and policy. Despite its potential, key questions remain-particularly whether genome editing can increase genetic diversity without causing unintended non-target impacts. Genome editing also provokes new perspectives on conservation practices where ecosystem-wide impact assessment, case-by-case evaluations, and post-release monitoring needs to be prioritized. Furthermore, conservation biotechnology is heavily funded through private funding showing varying stakeholder interest, which can lead to untraditional and less transparent research processes. Stakeholders, including local and indigenous people, are only to a certain degree involved, which may weaken inclusion of local knowledge and monitoring efforts. Finally, concerning governance and policy, there is an urgent need to develop more adequate regulation of conservation biotechnology, as environmental release of genome-edited animals challenges definitions and guidelines in current nature protection laws and GMO regulations. Based on our analysis, we outline key points for further investigation toward a more sustainable approach to conservation biotechnology.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":"5"},"PeriodicalIF":1.9,"publicationDate":"2026-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12881173/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146133004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The transgenic Vip3A poplar plant confers high resistance against Hyphantria cunea Drury.","authors":"Zhian Wang, Xiaoning Zhang, Ye Tang, Ziteng Xu, Yunjiao Xu, Jinmao Wang, Minsheng Yang, Jiahe Wu","doi":"10.1007/s11248-026-00480-1","DOIUrl":"10.1007/s11248-026-00480-1","url":null,"abstract":"<p><p>Poplar is severely damaged by Hyphantria cunea (fall webworm), which significantly reduces tree productivity. However, conventional pest management methods are largely ineffective against fall webworm infestation. In this study, we demonstrated that the Vip3A protein possesses high insecticidal activity against H. cunea by overexpressing a synthetic THI1-Vip3A gene in poplar plants. A dicot codon-optimized Vip3A gene, fused with the THI1 chloroplast signal peptide sequence, was chemically synthesized and introduced into the poplar cv. '741' genome via Agrobacterium-mediated transformation. PCR, RT-PCR, and ELISA analyses confirmed the integration and successful expression of the transgene at both the mRNA and protein levels. The Vip3A protein concentration in chloroplasts was approximately 4.8-fold higher than in the whole leaf extract, indicating that the Vip3A protein was successfully targeted to and accumulated within the chloroplasts by the THI1 signal peptide. Subsequently, four transgenic lines with high Vip3A expression were subjected to H. cunea infestation. Compared to wild-type plants, these four transgenic lines exhibited significantly higher resistance, resulting in pest mortality rates exceeding 95% and significantly reduced leaf damage. Together, these results indicate that Vip3A possesses high insecticidal activity against H. cunea. Therefore, transgenic THI1-Vip3A poplar plants can serve as valuable germplasm for breeding poplar cultivars with high resistance to H. cunea infestation.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":"4"},"PeriodicalIF":1.9,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146114260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of Forsythia intermedia pinoresinol lariciresinol reductase gene in Sesamum indicum increases secoisolariciresinol diglucoside content.","authors":"T Gayatri","doi":"10.1007/s11248-025-00479-0","DOIUrl":"10.1007/s11248-025-00479-0","url":null,"abstract":"<p><p>Sesame (Sesamum indicum), an important oilseed crop, is well known for its therapeutic values. It is one of the richest sources of polyunsaturated fatty acids and health beneficiary lignans. However, nutraceutical potential is restricted due to the presence of secoisolariciresinol diglucoside (SDG), a potent anticarcinogen, in imperceptible amount (~ 0.03 mg/25 g of seeds). Consequently, a logical strategy was implemented to augment the SDG content in sesame by heterologous expression of the pinoresinol-lariciresinol reductase (Fiplr) gene from Forsythia intermedia. Four individual transgenic lines were generated by Agrobacterium-mediated transformation of pCAMBIA/Fiplr gene cassette into sesame and was further confirmed by PCR and Southern blot analyses. Among the Individual transgenic lines, PT₁1.1 was found to demonstrate ~ 3.5-fold higher expression of plr transcript in comparison to other transformants and untransformed control. PT₁1.1 plant also showed ~ twofold increased PLR enzyme activity that led to ~ threefold increased accumulation of SDG content as compared to that in untransformed control when confirmed by the HPLC analyses. Thus, the study suggests that the presently developed transgenic sesame line may have the better dietary prospect for people suffering particularly from estrogen-induced cancers and can emerge having significant medical implications.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":"3"},"PeriodicalIF":1.9,"publicationDate":"2026-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146067293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}