Transgenic Research最新文献_第4页

An advanced cytosine base editor enabled the generation of cattle with a stop codon in the β-lactoglobulin gene. 一种先进的胞嘧啶碱基编辑器使牛在β-乳球蛋白基因中具有停止密码子。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2025-03-14 DOI: 10.1007/s11248-025-00435-y

Qiang Ding, Zhaokang Cui, Qianqian Shi, Yan Zhang, Nan He, Rihong Guo, Yu Tian, Shaoxian Cao, Jifeng Zhong, Huili Wang

{"title":"An advanced cytosine base editor enabled the generation of cattle with a stop codon in the β-lactoglobulin gene.","authors":"Qiang Ding, Zhaokang Cui, Qianqian Shi, Yan Zhang, Nan He, Rihong Guo, Yu Tian, Shaoxian Cao, Jifeng Zhong, Huili Wang","doi":"10.1007/s11248-025-00435-y","DOIUrl":"10.1007/s11248-025-00435-y","url":null,"abstract":"β-Lactoglobulin (BLG) is an allergen present in milk that can induce an acute immune response in certain individuals. The successful use of cytosine base editors (CBEs) can introduce stop codons into premature mRNA, thereby generating animals with disrupted genes that negatively regulate target traits. In this study, we employed a CBE system to target the major milk allergen BLG in bovine embryos, mammary epithelial cells, and live cattle. First, the precise single-base editing of the BLG gene in bovine embryos was achieved by designing an effective sgRNA to induce a c.61C > T substitution in the coding region, converting codon 21Gln (p.21Gln) to a premature stop codon. Sanger sequencing revealed an editing efficiency of 83.3% (20 out of 24 embryos), including two homozygous edits. Second, a bovine mammary epithelial cell line harboring BLG edits was constructed using the same CBE system. Sequencing showed that the designed sgRNA1 enabled the simultaneous conversion of three consecutive cytosines (c.59-61CCC > TTT) to thymines. At position c.61, single-cell clones exhibited monoallelic or biallelic editing (BLGc.61C > T), with monoallelic edits at positions c.59 and c.60 (CC > TT). Gene expression analysis confirmed that the BLGc.61C > T mutation effectively suppressed BLG expression at both the mRNA and protein levels, even in monoallelically edited cells. Finally, we successfully generated a heterozygous BLGc.61C > T single-base-edited dairy cow that despite its heterozygosity, showed significantly reduced BLG expression in the mammary epithelial cells and milk. Collectively, this study demonstrates the feasibility of using CBEs to disrupt BLG expression in dairy cows and provides a foundation for application in generating hypoallergenic dairy products.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"34 1","pages":"14"},"PeriodicalIF":2.7,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143634684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome editing research initiatives and regulatory landscape of genome edited crops in India. 印度基因组编辑研究计划和基因组编辑作物的监管前景。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2025-03-13 DOI: 10.1007/s11248-025-00432-1

Neha Sharma, Kanika Thakur, Rasna Zinta, Vikas Mangal, Dalamu, Jagesh Kumar Tiwari, Salej Sood, Som Dutt, Vinod Kumar, Brajesh Singh, Ajay Kumar Thakur

{"title":"Genome editing research initiatives and regulatory landscape of genome edited crops in India.","authors":"Neha Sharma, Kanika Thakur, Rasna Zinta, Vikas Mangal, Dalamu, Jagesh Kumar Tiwari, Salej Sood, Som Dutt, Vinod Kumar, Brajesh Singh, Ajay Kumar Thakur","doi":"10.1007/s11248-025-00432-1","DOIUrl":"10.1007/s11248-025-00432-1","url":null,"abstract":"Food and nutritional security are the top priorities in Indian agriculture. Exponential population growth coupled with climate change effects has become a serious challenge for sustainable agriculture. Genome editing has revolutionized the agricultural sector because of its ability to create precise, stable and predictable modifications in the genome and therefore, offers great opportunities for crop improvement in India. However, for harvesting the real benefits of this technology in agriculture sector, there is a strong need of creating awareness among the end users and development of suitable policies for regularization of genome edited products. Many regulatory agencies around the world have been modernizing their regulatory approaches to be more risk proportionate and to reflect a more science-based approach. In this article, recent research initiatives and developments undertaken by different Indian institutes/organizations for the genetic improvement of agricultural and horticultural crops via genome editing technologies are summarized. Furthermore, to benefit from this potential technology in our country, regulatory policies must be clear, science-based and proportionate. Therefore, in the present review, the regulatory policies related to the genome editing of crop products in India are discussed in detail. This review will sensitize researchers and stakeholders to the application of genome editing techniques in crop improvement and various biosafety committees involved in the development and regulation of genome edited crops.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"34 1","pages":"13"},"PeriodicalIF":2.7,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive transcriptomic analysis of myostatin-knockout pigs: insights into muscle growth and lipid metabolism. 肌生成抑制素敲除猪的综合转录组学分析：洞察肌肉生长和脂质代谢。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2025-02-20 DOI: 10.1007/s11248-025-00431-2

Nasar Khan, Zhouyan Li, Akbar Ali, Biaohu Quan, Jindan Kang, Munib Ullah, Xi-Jun Yin, Muhammad Shafiq

{"title":"Comprehensive transcriptomic analysis of myostatin-knockout pigs: insights into muscle growth and lipid metabolism.","authors":"Nasar Khan, Zhouyan Li, Akbar Ali, Biaohu Quan, Jindan Kang, Munib Ullah, Xi-Jun Yin, Muhammad Shafiq","doi":"10.1007/s11248-025-00431-2","DOIUrl":"10.1007/s11248-025-00431-2","url":null,"abstract":"Pigs are a vital source of protein worldwide, contributing approximately 43% of global meat production. Recent genetic advancements in the myostatin (MSTN) gene have facilitated the development of double-muscling traits in livestock. In this study, we investigate the transcriptomic profiles of second-generation MSTN-knockout (MSTN-/-) pigs, generated through CRISPR/Cas9 gene editing and somatic cell nuclear transfer (SCNT). Using RNA sequencing, we compared the transcriptomic landscapes of muscle tissues from MSTN-/- pigs and wild-type (WT) counterparts. The sequencing yielded an average unique read mapping rate of 86.7% to the Sus scrofa reference genome. Our analysis revealed 15,142 differentially expressed genes (DEGs), including 121 novel genes, with 2554 genes upregulated and 1629 downregulated in the MSTN-/- group relative to the wild-type group. Notable transcriptomic changes were identified in genes associated with muscle development, lipid metabolism, and other physiological processes. These findings provide valuable insights into the molecular consequences of MSTN inactivation, with potential applications in the optimization of livestock breeding and advancements in biomedical research.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"34 1","pages":"12"},"PeriodicalIF":2.7,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143469350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of transgene on salt tolerance of tobacco. 转基因对烟草耐盐性的影响。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2025-01-28 DOI: 10.1007/s11248-025-00430-3

Jie Sun, Yan Dong, Yuemei Meng, Jingran Bi, Hongmei Liu, Junjie Ren, Jinmao Wang, Yachao Ren, Minsheng Yang

{"title":"Effect of transgene on salt tolerance of tobacco.","authors":"Jie Sun, Yan Dong, Yuemei Meng, Jingran Bi, Hongmei Liu, Junjie Ren, Jinmao Wang, Yachao Ren, Minsheng Yang","doi":"10.1007/s11248-025-00430-3","DOIUrl":"10.1007/s11248-025-00430-3","url":null,"abstract":"To explore the effects of salt-tolerance gene accumulation on salt tolerance in transgenic plant, we used four types of plant expression vector (N27, N28, N29, and N30) carrying mtlD, mtlD + gutD, mtlD + gutD + BADH, mtlD + gutD + BADH + sacB genes respectively, to transform tobacco through Agrobacterium-mediated method. Transgenic lines were identified through polymerase chain reaction (PCR) detection. Transgenic lines and non-transgenic plant (CK) were subjected to 6‰ sodium chloride solution stress; then, fluorescence quantitative PCR (FQ-PCR) and salt tolerance indexes were used to assess characteristics. PCR showed the exogenous genes had been integrated into the tobacco genome. FQ-PCR showed under clean water treatment the target genes were expressed in all transgenic plants at the transcriptional level. The transcript abundances of target genes changed with the number of genes increased, and improved following salt stress. Comparative analyses of salt tolerance indexes showed height growth, biomass (except for N29), chlorophyll content, net photosynthetic rate, Fv/Fm, and PI of all transgenic plants and CK were lower under salt stress than under clean water treatment, to varying degrees. However, the descent ratio was smaller in transgenic plants. A comprehensive evaluation of multiple salt-tolerance indicators performed using the membership function method showed the average salt tolerance of each vector transgenic line was higher than that of CK, and salt tolerance was greater in transgenic polyvalent gene lines than in transgenic monovalent gene lines. The average salt tolerance was N29 > N28 > N30 > N27 > CK. This study provides a theoretical and practical reference for salt tolerance breeding in other plants.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"34 1","pages":"11"},"PeriodicalIF":2.7,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143053782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NtLPA1 overexpression regulates the growth of tobacco and enhances resistance to blight. NtLPA1过表达调控烟草生长，增强烟草抗枯萎病能力。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2025-01-09 DOI: 10.1007/s11248-024-00420-x

Guiqin Shi, Yanxiao Bu, Lei Chi, Xifeng Zhang, Yuqing Meng, Shijie Zhang, Geng Tian

引用次数: 0

Expression of Agrobacterium Isopentenyl transferase (IPT) gene in wheat improves drought tolerance. 异戊烯基转移酶（IPT）基因在小麦中的表达提高了小麦的抗旱性。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2025-01-09 DOI: 10.1007/s11248-024-00421-w

Sidra Ijaz, Aftab Bashir, Kauser A Malik

{"title":"Expression of Agrobacterium Isopentenyl transferase (IPT) gene in wheat improves drought tolerance.","authors":"Sidra Ijaz, Aftab Bashir, Kauser A Malik","doi":"10.1007/s11248-024-00421-w","DOIUrl":"https://doi.org/10.1007/s11248-024-00421-w","url":null,"abstract":"Drought, as an abiotic stressor, globally limits cereal productivity, leading to early aging of leaves and lower yields. The expression of the isopentenyl transferase (IPT) gene, which is involved in cytokinin (CK) biosynthesis, can delay drought-induced leaf senescence. In this study, the Agrobacterium Isopentenyl transferase (IPT) gene was introduced into two local hexaploid wheat cultivars, NR-421 and FSD-2008. The expression cassette was developed containing the IPT gene under transcriptional regulation of the stress-inducible promoter 'Dehydrin,' sourced from Hordeum vulgare. The gene expression cassette was assembled in pSB219M, a modified transformation vector for monocots, equipped with both an antibiotic (spectinomycin) and an herbicide selection marker (BASTA). Initial screening of transgenic plants involved BASTA selection (2 and 3 mg/L) and was subsequently confirmed through PCR analysis. The transformation efficiencies of NR-421 and FSD-2008 were 0.4% and 0.3%, respectively. The qRT-PCR analysis under stress conditions showed a 13.5-fold higher expression of the IPT gene in T2 transgenic plants of NR-421 and a 5.8-fold higher expression in those of FSD-2008 than in non-transgenic controls. Under stress conditions, the wheat transgenic plants exhibited increased chlorophyll and relative water content. Additionally, for total soluble proteins, two transgenic lines from the NR-421 variety showed a significant increase, whereas no notable change was observed in the FSD-2008 transgenics. Moreover, the transgenic lines displayed increased plant height, higher fresh and dry biomass, and increased seed weight compared to the non-transgenic controls. These findings highlight that stress-inducible expression of the IPT gene in wheat leads to enhanced grain yield and subsequently improved drought tolerance.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"34 1","pages":"7"},"PeriodicalIF":2.7,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142955703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resistance of Populus davidiana × P. bolleana overexpressing cinnamoyl-CoA reductase gene to Lymantria dispar larvae. 山杨的抗性研究。过表达肉桂酰辅酶a还原酶基因对异毒蛾幼虫的影响。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2025-01-09 DOI: 10.1007/s11248-024-00426-5

Ye Li, Ruiqiong Zhang, Lili Sun, Chuanwang Cao

引用次数: 0

Development of a new flippase-dependent mouse model for red fluorescence-based isolation of KRAS^G12D oncogene-expressing tumor cells. 建立一种新的翻转酶依赖小鼠模型，用于红色荧光分离表达KRASG12D癌基因的肿瘤细胞。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2025-01-09 DOI: 10.1007/s11248-024-00429-2

Dusan Hrckulak, Jakub Onhajzer, Michaela Krausova, Monika Stastna, Vitezslav Kriz, Lucie Janeckova, Vladimir Korinek

{"title":"Development of a new flippase-dependent mouse model for red fluorescence-based isolation of KRASG12D oncogene-expressing tumor cells.","authors":"Dusan Hrckulak, Jakub Onhajzer, Michaela Krausova, Monika Stastna, Vitezslav Kriz, Lucie Janeckova, Vladimir Korinek","doi":"10.1007/s11248-024-00429-2","DOIUrl":"10.1007/s11248-024-00429-2","url":null,"abstract":"Proto-oncogene KRAS, GTPase (KRAS) is one of the most intensively studied oncogenes in cancer research. Although several mouse models allow for regulated expression of mutant KRAS, selective isolation and analysis of transforming or tumor cells that produce the KRAS oncogene remains a challenge. In our study, we present a knock-in model of oncogenic variant KRASG12D that enables the \"activation\" of KRASG12D expression together with production of red fluorescent protein tdTomato. Both proteins are expressed from the endogenous Kras locus after recombination of a transcriptional stop box in the genomic DNA by the enzyme flippase (Flp). We have demonstrated the functionality of the allele termed RedRas (abbreviated KrasRR) under in vitro conditions with mouse embryonic fibroblasts and organoids and in vivo in the lung and colon epithelium. After recombination with adenoviral vectors carrying the Flp gene, the KrasRR allele itself triggers formation of lung adenomas. In the colon epithelium, it causes the progression of adenomas that are triggered by the loss of tumor suppressor adenomatous polyposis coli (APC). Importantly, cells in which recombination has successfully occurred can be visualized and isolated using the fluorescence emitted by tdTomato. Furthermore, we show that KRASG12D production enables intestinal organoid growth independent of epidermal growth factor (EGF) signaling and that the KRASG12D function is effectively suppressed by specific inhibitor MRTX1133.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"34 1","pages":"9"},"PeriodicalIF":2.7,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11717838/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142955700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring genetic mapping and co-expression patterns to illuminate significance of Tbx20 in cardiac biology. 探索基因定位和共表达模式，阐明Tbx20在心脏生物学中的意义。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2025-01-07 DOI: 10.1007/s11248-024-00423-8

Dezhong Zhang, Xiao Shang, Quanquan Ji, Li Niu

{"title":"Exploring genetic mapping and co-expression patterns to illuminate significance of Tbx20 in cardiac biology.","authors":"Dezhong Zhang, Xiao Shang, Quanquan Ji, Li Niu","doi":"10.1007/s11248-024-00423-8","DOIUrl":"https://doi.org/10.1007/s11248-024-00423-8","url":null,"abstract":"The transcription factor Tbx20 is integral to heart development and plays a significant role in various cardiac diseases. Despite its established importance, the regulatory mechanisms and functional significance of Tbx20 remain incompletely understood. To elucidate these mechanisms, we initially conducted eQTL mapping to identify genetic loci associated with Tbx20 expression in heart tissue from BXD mice. Co-expression and enrichment analyses revealed pathways linked to Tbx20, including dilated cardiomyopathy, hypertrophic cardiomyopathy, and FoxO signaling. Additionally, protein-protein interaction studies identified essential cardiac proteins, such as Myl2 and Myl7, along with upstream regulators like Mef2c. To validate our bioinformatic findings, we performed quantitative reverse transcription polymerase chain reaction (qRT-PCR) to assess the relative mRNA expression levels of TBX20 and Mef2c in the heart tissues of BXD mice compared to their parental strains (B6 and D2). Our results demonstrated significant up-regulation of both TBX20 and Mef2c in the BXD group relative to the parental strains. Conversely, both genes were down-regulated in B6, D2, Control, and Treatment groups when compared to BXD mice. These findings confirm the predicted regulatory roles of TBX20 and Mef2c in cardiac development as suggested by our initial analyses.This study not only reinforces the critical role of Tbx20 in cardiac gene regulation but also highlights its potential as a therapeutic target for cardiovascular disorders. Further investigations into Tbx20 and its interactions will enhance our understanding of heart biology and contribute to the development of targeted therapies for heart diseases.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"34 1","pages":"5"},"PeriodicalIF":2.7,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142955702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Do confined field trials add value for the environment risk assessment of genetically modified Brassica napus L. in Japan? 日本转基因甘蓝型油菜的限制性田间试验是否增加了环境风险评估的价值？

IF 2.7 3区生物学

Transgenic Research Pub Date : 2025-01-07 DOI: 10.1007/s11248-024-00425-6

Kei Takamoto, Kaori Inazu, Shuichi Nakai, Koichi Inoue, Mai Tsuda

{"title":"Do confined field trials add value for the environment risk assessment of genetically modified Brassica napus L. in Japan?","authors":"Kei Takamoto, Kaori Inazu, Shuichi Nakai, Koichi Inoue, Mai Tsuda","doi":"10.1007/s11248-024-00425-6","DOIUrl":"10.1007/s11248-024-00425-6","url":null,"abstract":"The environmental risk assessment (ERA) of genetically modified (GM) crops in Japan requires collecting data from a comparative study of a GM and non-GM control in an in-country confined field trial (CFT). This in-country CFT requirement is used to address concerns that differences in the local environmental conditions may lead to differences in growth and/or risks of GM crops. However, this requirement for in-country CFT has recently been exempted for certain GM maize and GM cotton traits, and instead CFT data from other countries are used to inform the ERA of these GM events. However, in-country CFTs continue to be required for GM B. napus. Our objective is to assess whether using B. napus as a host crop increases the potential for differences between GM B. napus and conventional B. napus that may have an impact on biodiversity occurring only under the Japanese environment. In this paper agronomic data was compiled from seven local CFTs of GM B. napus events to assess the potential for differences between GM and non-GM B. napus for three key areas; competitiveness, potential to produce harmful substances, and outcrossing. Considering these elements, the need for conducting CFTs locally for ERA of future GM B. napus traits is discussed. The assessment concluded that conducting CFT locally is not necessary for GM B. napus events if traits do not bring competitive advantage or produce harmful substances only under Japanese environment.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"34 1","pages":"6"},"PeriodicalIF":2.7,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11706835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142955701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0