Transgenic Research最新文献_第4页

Long-term (10-year) monitoring of transposon-mediated transgenic cattle. 对转座子介导的转基因牛进行长期（10 年）监测。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-10-01 Epub Date: 2024-08-28 DOI: 10.1007/s11248-024-00401-0

Soo-Young Yum, Bae Young Choi, Gyeong-Min Gim, Kyeong-Hyeon Eom, Seong-Beom Lee, Daehyun Kim, Euntaek Lim, Do-Yoon Kim, Seong-Eun Heo, Donghwan Shim, Goo Jang

{"title":"Long-term (10-year) monitoring of transposon-mediated transgenic cattle.","authors":"Soo-Young Yum, Bae Young Choi, Gyeong-Min Gim, Kyeong-Hyeon Eom, Seong-Beom Lee, Daehyun Kim, Euntaek Lim, Do-Yoon Kim, Seong-Eun Heo, Donghwan Shim, Goo Jang","doi":"10.1007/s11248-024-00401-0","DOIUrl":"10.1007/s11248-024-00401-0","url":null,"abstract":"The production of transgenic animals using non-viral methods has raised questions regarding their long-term health and genomic stability. In this study, we evaluated these aspects in transgenic cattle over ten years, using transposon-mediated gene transfer. Our longitudinal analysis included a comprehensive health assessment and whole-genome DNA resequencing. We found no significant alterations in physiological parameters or health complications in transposon-mediated transgenic cattle that exceeded 10 years of age. Genomic analysis revealed that the rates of somatic mutations and copy number variations in transgenic cattle were comparable to those in non-transgenic cattle. Furthermore, structural variants were infrequent, suggesting that transposon-mediated gene insertion did not compromise genomic integrity. These findings highlight the viability of transposon systems for generating transgenic livestock, potentially expanding their applications in agriculture and biotechnology. This study contributes significantly to our understanding of the long-term implications of transgenesis in large animals and supports the safety and stability of this method.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":" ","pages":"503-512"},"PeriodicalIF":2.7,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11588892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142081705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OsHRZ1 negatively regulates rice resistant to Magnaporthe oryzae infection by targeting OsVOZ2. OsHRZ1 通过靶向 OsVOZ2 负向调控水稻对 Magnaporthe oryzae 感染的抗性。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-10-01 Epub Date: 2024-09-27 DOI: 10.1007/s11248-024-00415-8

Jia-Ying Sun, Zeng-Ran Zhou, Yu-Qi Wang, Dong-Yu Zhu, Dian-Rong Ma

{"title":"OsHRZ1 negatively regulates rice resistant to Magnaporthe oryzae infection by targeting OsVOZ2.","authors":"Jia-Ying Sun, Zeng-Ran Zhou, Yu-Qi Wang, Dong-Yu Zhu, Dian-Rong Ma","doi":"10.1007/s11248-024-00415-8","DOIUrl":"10.1007/s11248-024-00415-8","url":null,"abstract":"Rice blast disease caused by Magnaporthe oryzae significantly reduces yield production. Blast resistance is closely associated with iron (Fe) status, but the mechanistic basis linking iron status to immune function in rice remains largely unknown. Here, iron-binding haemerythrin RING ubiquitin ligases OsHRZ1 was confirmed to play key roles in iron-mediated rice blast resistance. The expression of OsHRZ1 was suppressed by M. oryzae inoculation and high iron treatment. Both mutants of OsHRZ1 enhanced rice resistance to M. oryzae. OsPR1a was up-regulated in OsHRZ1 mutants. Yeast two-hybrid, bimolecular fluorescence complementation, and Co-IP assay results indicated that OsHRZ1 interacts with Vascular Plant One Zinc Finger 2 (OsVOZ2) in the nucleus. Additionally, the vitro ubiquitination assay indicated that OsHRZ1 can ubiquitinate OsVOZ2 and mediate the degradation of OsVOZ2. The mutants of OsVOZ2 showed reduced resistance to M. oryzae and down-regulated the expression of OsPR1a. Yeast one-hybrid, EMSA, and dual-luciferase reporter assay results indicated that OsVOZ2 directly binds to the promoter of OsPR1a, activating its expression. In summary, OsHRZ1 plays an important role in rice disease resistance by mediated degradation of OsVOZ2 thus shaping PR gene expression dynamics in rice cells. This highlights an important link between iron signaling and rice pathogen defenses.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":" ","pages":"489-501"},"PeriodicalIF":2.7,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional analysis of root-preferential oil palm metallothionein promoter in tobacco. 烟草根系偏好油棕金属硫蛋白启动子的功能分析

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-10-01 Epub Date: 2024-08-09 DOI: 10.1007/s11248-024-00396-8

Subhi Siti Masura, Noor Azmi Shaharuddin, Mat Yunus Abdul Masani, Kuang-Lim Chan, Eng-Ti Leslie Low, Pek-Lan Chan, Abdul Rahman Siti Rahmah, Nadzirah Amiruddin, Mohd Puad Abdullah, Azzreena Mohamad Azzeme, Ghulam Kadir Ahmad Parveez, Omar Abd Rasid

{"title":"Functional analysis of root-preferential oil palm metallothionein promoter in tobacco.","authors":"Subhi Siti Masura, Noor Azmi Shaharuddin, Mat Yunus Abdul Masani, Kuang-Lim Chan, Eng-Ti Leslie Low, Pek-Lan Chan, Abdul Rahman Siti Rahmah, Nadzirah Amiruddin, Mohd Puad Abdullah, Azzreena Mohamad Azzeme, Ghulam Kadir Ahmad Parveez, Omar Abd Rasid","doi":"10.1007/s11248-024-00396-8","DOIUrl":"10.1007/s11248-024-00396-8","url":null,"abstract":"Root-specific or preferential promoters are essential to genetically modify plants with beneficial root traits. We have characterised the promoter from an oil palm metallothionein gene (EgMT) and performed a serial 5' deletion analysis to identify the region(s) essential for transgenes expression in roots. Stable functional characterisation of tobacco transgenic lines using the T1 generation showed that a deletion construct, designated as RSP-2D (1107 bp), directed strong GUS expression at all stages of root development, particularly in mature roots. Other constructs, RSP-2A (2481 bp) and RSP-2C (1639 bp), drove GUS expression in roots with an intensity lower than RSP-2D. The promoter activity was also detectable in seed pods and immature seeds, albeit at lower levels than CaMV35S. The promoter activity may also be induced by wounding as intact GUS staining was observed at the flower- and leaf-cutting sites of T1 samples carrying either RSP-2C or RSP-2D constructs. The promoter sequence contains cis-acting elements that may act as negative regulators and be responsible for root specificity. The results further indicated that the 5' UTR and ATATT sequences are essential for strong promoter activity. This study highlights the potential of RSP-2D promoter as a tool for modifying root traits through genetic engineering.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":" ","pages":"383-397"},"PeriodicalIF":2.7,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of dehydroascorbate reductase gene IbDHAR1 improves the tolerance to abiotic stress in sweet potato. 过表达脱氢抗坏血酸还原酶基因 IbDHAR1 可提高甘薯对非生物胁迫的耐受性。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-10-01 Epub Date: 2024-09-09 DOI: 10.1007/s11248-024-00408-7

Qirui Cheng, Xuan Zou, Yuan Wang, Zhe Yang, Xiangpo Qiu, Sijie Wang, Yanxin Yang, Dongjing Yang, Ho Soo Kim, Xiaoyun Jia, Lingzhi Li, Sang-Soo Kwak, Wenbin Wang

{"title":"Overexpression of dehydroascorbate reductase gene IbDHAR1 improves the tolerance to abiotic stress in sweet potato.","authors":"Qirui Cheng, Xuan Zou, Yuan Wang, Zhe Yang, Xiangpo Qiu, Sijie Wang, Yanxin Yang, Dongjing Yang, Ho Soo Kim, Xiaoyun Jia, Lingzhi Li, Sang-Soo Kwak, Wenbin Wang","doi":"10.1007/s11248-024-00408-7","DOIUrl":"10.1007/s11248-024-00408-7","url":null,"abstract":"Dehydroascorbate reductase (DHAR), an indispensable enzyme in the production of ascorbic acid (AsA) in plants, is vital for plant tolerance to various stresses. However, there is limited research on the stress tolerance functions of DHAR genes in sweet potato (Ipomoea batatas [L.] Lam). In this study, the full-length IbDHAR1 gene was cloned from the leaves of sweet potato cultivar Xu 18. The IbDHAR1 protein is speculated to be located in both the cytoplasm and the nucleus. As revealed by qRT-PCR, the relative expression level of IbDHAR1 in the proximal storage roots was much greater than in the other tissues, and could be upregulated by high-temperature, salinity, drought, and abscisic acid (ABA) stress. The results of pot experiments indicated that under high salinity and drought stress conditions, transgenic Arabidopsis and sweet potato plants exhibited decreases in H2O2 and MDA levels. Conversely, the levels of antioxidant enzymes APX, SOD, POD, and ACT, and the content of DHAR increased. Additionally, the ratio of AsA/DHA was greater in transgenic lines than in the wild type. The results showed that overexpression of IbDHAR1 intensified the ascorbic acid-glutathione cycle (AsA-GSH) and promoted the activity of the related antioxidant enzyme systems to improve plant stress tolerance and productivity.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":" ","pages":"427-443"},"PeriodicalIF":2.7,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142155038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Mouse Model for Conditional Expression of Activated β-Catenin in Epidermal Keratinocytes. 表皮角质形成细胞中活化β-Catenin条件表达的小鼠模型

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-10-01 Epub Date: 2024-08-07 DOI: 10.1007/s11248-024-00402-z

Vineet K Maurya, Yan Ying, John P Lydon

{"title":"A Mouse Model for Conditional Expression of Activated β-Catenin in Epidermal Keratinocytes.","authors":"Vineet K Maurya, Yan Ying, John P Lydon","doi":"10.1007/s11248-024-00402-z","DOIUrl":"10.1007/s11248-024-00402-z","url":null,"abstract":"We report the generation and characterization of the K5: CAT bigenic mouse in which the constitutively activated form of β-catenin (ΔN89 β-catenin) is conditionally expressed in cytokeratin-5 (K5) positive epidermal keratinocytes. Following short-term doxycycline intake during the telogen resting phase, the adult K5: CAT bigenic develops enlarged pilosebaceous units that expand deep into the dermis, an expansion usually observed during the anagen growth phase. Prolonged doxycycline treatment results in significant thickening and folding of the K5: CAT epidermis. During this persistent induction period, there is clear evidence of increased keratinocyte proliferation, particularly in the epidermal basal cell layer and the outer root sheath of the hair follicle. This unscheduled increase in cellular proliferation likely explains the decrease in hair density observed in the K5: CAT mouse following persistent doxycycline intake. Numerous hyperplastic endometrioid cysts, which display cornification toward their lumens, are also observed during this treatment period. Remarkably, de-induction of ΔN89 β-catenin expression through doxycycline withdrawal results in a marked reversal of the skin phenotype, suggesting that these morphological changes are dependent on continued signaling by β-catenin and/or its downstream molecular mediators. Joining a small group of mouse models for conditional β-catenin signaling, our K5: CAT mouse model will be particularly useful in identifying those molecular mediators of β-catenin that are responsible for initiating and maintaining these phenotypic responses in the K5: CAT skin. Such studies are predicted to shed more light on β-catenin signaling in epidermal epithelial morphogenesis, hair follicle cycling, and hair growth pathologies.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":" ","pages":"513-525"},"PeriodicalIF":2.7,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11588969/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141898312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mosquito pollination of plants: an overview of their role and an assessment of the possible contribution of disease vectors. 蚊子为植物授粉：概述其作用并评估病媒的可能贡献。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-10-01 Epub Date: 2024-08-22 DOI: 10.1007/s11248-024-00394-w

Woodbridge A Foster

{"title":"Mosquito pollination of plants: an overview of their role and an assessment of the possible contribution of disease vectors.","authors":"Woodbridge A Foster","doi":"10.1007/s11248-024-00394-w","DOIUrl":"10.1007/s11248-024-00394-w","url":null,"abstract":"Mosquitoes visit flowers to obtain sugar or other nutrients and therefore possibly serve as major or minor pollinators of some plant species. They also often derive plant nutrients from other sources, such as extrafloral nectaries and honeydew. In a few cases, the plant-mosquito relationship is close, and mosquito pollination has been confirmed. Most plant species visited by mosquitoes, however, appear to depend on multiple means of pollination, particularly other flower-feeding insects. In addition, most mosquito species visit the flowers of many kinds of plants, possibly dispersing pollen in both biologically meaningful and irrelevant ways. This apparent lack of selectivity by both plants and mosquitoes liberates each of them from dependence on an unreliable pollen vehicle or nutrient source. A hypothetical pollinating role for the two top vectors of devastating human-disease pathogens, Anopheles gambiae or Aedes aegypti, relies on indirect evidence. So far, this evidence suggests that their participation in pollen transfer of native, introduced, or beneficial plants is negligible. The few plant species likely to be pollinated by these vectors are mostly invasive, harmful weeds associated with humans. That conclusion draws support from four characteristics of these vectors: (1) the numerous alternative potential pollinators of the flowers they visit; (2) their common use of diverse non-floral sources of nutrients; (3) the females' infrequent sugar feeding and heavy reliance on human blood for energy; and (4) their relatively low population densities. From these traits it follows that focused suppression or elimination of these two vectors, by whatever means, is highly unlikely to have adverse effects on pollination in endemic biotic communities or on ornamental plants or food crops.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":" ","pages":"297-322"},"PeriodicalIF":2.7,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11588815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142018707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tracing scientific and technological development in genetically modified crops 追踪转基因作物的科技发展情况

IF 3 3区生物学

Transgenic Research Pub Date : 2024-09-18 DOI: 10.1007/s11248-024-00412-x

Anurag Kanaujia, Solanki Gupta

{"title":"Tracing scientific and technological development in genetically modified crops","authors":"Anurag Kanaujia, Solanki Gupta","doi":"10.1007/s11248-024-00412-x","DOIUrl":"https://doi.org/10.1007/s11248-024-00412-x","url":null,"abstract":"Genetically Modified (GM) Organisms have been used in various domains since their introduction in the 1980s. According to ISAAA data, the use of GM crops in agriculture has also increased significantly in the past 30 years. However, even after 3 decades of commercialisation, GM crops are still surrounded with controversies with different countries adopting varying approaches to their introduction in the consumer markets, owing to different stances of various stakeholders. Motivated by this multitude of opinions, and absence of knowledge mapping, this study has undertaken scientometric analysis of the publication (Web of Science) and patent (Lens.org) data about genetically modified technology use in agriculture to explore the changing knowledge patterns and technological advancements in the area. It explores both scientific and technological perspectives regarding the use of Genetically Modified Crops, by using publication as well as patent data. The findings of this study highlight the major domains of research, technology development, and leading actors in the ecosystem. These findings can be helpful in taking effective policy decisions, and furthering the research activities. It presents a composite picture using both publications and patent data. Further, it will be of utility to explore the other technologies which are replacing GM technology in agriculture in future studies.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"49 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142267168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bending away from salt: a SMB-AUX1 story 远离盐：SMB-AUX1 的故事

IF 3 3区生物学

Transgenic Research Pub Date : 2024-09-17 DOI: 10.1007/s11248-024-00409-6

Hamza Sohail, Iqra Noor, Xuewen Xu, Xuehao Chen, Xiaodong Yang

引用次数: 0

Field performance and nitrous oxide emissions of transgenic nitrogen use efficient rice lines cultivated in tropical paddy fields 热带稻田中种植的转基因氮利用高效水稻品系的田间表现和一氧化二氮排放量

IF 3 3区生物学

Transgenic Research Pub Date : 2024-09-13 DOI: 10.1007/s11248-024-00410-z

Atmitri Sisharmini, Anicetus Wihardjaka, Wening Enggarini, Aniversari Apriana, Aris Hairmansis, Bahagiawati Amirhusin

{"title":"Field performance and nitrous oxide emissions of transgenic nitrogen use efficient rice lines cultivated in tropical paddy fields","authors":"Atmitri Sisharmini, Anicetus Wihardjaka, Wening Enggarini, Aniversari Apriana, Aris Hairmansis, Bahagiawati Amirhusin","doi":"10.1007/s11248-024-00410-z","DOIUrl":"https://doi.org/10.1007/s11248-024-00410-z","url":null,"abstract":"Nitrogen (N) fertilizers make up the majority of the input used in rice production, and their excess application leads to significant environmental pollution. Developing rice varieties with improved nitrogen use efficiency (NUE) is essential to maintain the sustainability of rice production. This study aims to evaluate the performance of transgenic Oryza sativa japonica cv. Kitaake expressing the barley (Hordeum vulgare) alanine aminotransferase (HvAlaAT) gene in response to different levels of N fertilizer application under tropical paddy field conditions. Results from this study demonstrate that transgenic nitrogen use efficient Kitaake rice (Kitaake NUE) displays a grain yield increase of up to 41% compared to Kitaake null. Transgenic Kitaake NUE expressing the HvAlaAT gene displays a higher N uptake and achieves a higher nitrogen use efficiency compared to control plants while maintaining lower nitrous oxide (N2O) fluxes. The reduction in N2O emissions in Kitaake NUE compared to Kitaake null ranges from 37.5 to 96.3%. The transgenic Kitaake NUE used in this study has potential as a donor to improve the nitrogen use efficiency of indica rice for better adaptability to tropical conditions.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"18 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142206160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NORE1A loss promotes MASLD/MASH NORE1A 缺失会促进 MASLD/MASH

IF 3 3区生物学

Transgenic Research Pub Date : 2024-09-09 DOI: 10.1007/s11248-024-00407-8

Howard Donninger, Katherine Hobbing, Gavin E. Arteel, Geoffrey J. Clark

引用次数: 0