Transgenic Research最新文献_第4页

Transgenic expression of artificial microRNA targeting soybean mosaic virus P1 gene confers virus resistance in plant. 转基因表达靶向大豆花叶病毒 P1 基因的人工 microRNA 可使植物产生抗病毒性。

IF 3 3区生物学

Transgenic Research Pub Date : 2024-06-01 Epub Date: 2024-06-06 DOI: 10.1007/s11248-024-00388-8

Muhammad Faizan Latif, Jingquan Tan, Wang Zhang, Wenxuan Yang, Tinghui Zhuang, Wenlong Lu, Yanglin Qiu, Xinying Du, Xinjian Zhuang, Tong Zhou, Jiban K Kundu, Jinlong Yin, Kai Xu

{"title":"Transgenic expression of artificial microRNA targeting soybean mosaic virus P1 gene confers virus resistance in plant.","authors":"Muhammad Faizan Latif, Jingquan Tan, Wang Zhang, Wenxuan Yang, Tinghui Zhuang, Wenlong Lu, Yanglin Qiu, Xinying Du, Xinjian Zhuang, Tong Zhou, Jiban K Kundu, Jinlong Yin, Kai Xu","doi":"10.1007/s11248-024-00388-8","DOIUrl":"10.1007/s11248-024-00388-8","url":null,"abstract":"RNA silencing is an innate immune mechanism of plants against invasion by viral pathogens. Artificial microRNA (amiRNA) can be engineered to specifically induce RNA silencing against viruses in transgenic plants and has great potential for disease control. Here, we describe the development and application of amiRNA-based technology to induce resistance to soybean mosaic virus (SMV), a plant virus with a positive-sense single-stranded RNA genome. We have shown that the amiRNA targeting the SMV P1 coding region has the highest antiviral activity than those targeting other SMV genes in a transient amiRNA expression assay. We transformed the gene encoding the P1-targeting amiRNA and obtained stable transgenic Nicotiana benthamiana lines (amiR-P1-3-1-2-1 and amiR-P1-4-1-2-1). Our results have demonstrated the efficient suppression of SMV infection in the P1-targeting amiRNA transgenic plants in an expression level-dependent manner. In particular, the amiR-P1-3-1-2-1 transgenic plant showed high expression of amiR-P1 and low SMV accumulation after being challenged with SMV. Thus, a transgenic approach utilizing the amiRNA technology appears to be effective in generating resistance to SMV.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141261809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Introgression of Δ¹-pyrroline-5-carboxylate synthetase (PgP5CS) confers enhanced resistance to abiotic stresses in transgenic tobacco. Δ1-吡咯啉-5-羧酸合成酶（PgP5CS）的导入增强了转基因烟草对非生物胁迫的抗性。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-06-01 Epub Date: 2024-05-13 DOI: 10.1007/s11248-024-00385-x

Gothandapani Sellamuthu, Avijit Tarafdar, Rahul Singh Jasrotia, Minakshi Chaudhary, Harinder Vishwakarma, Jasdeep C Padaria

{"title":"Introgression of Δ1-pyrroline-5-carboxylate synthetase (PgP5CS) confers enhanced resistance to abiotic stresses in transgenic tobacco.","authors":"Gothandapani Sellamuthu, Avijit Tarafdar, Rahul Singh Jasrotia, Minakshi Chaudhary, Harinder Vishwakarma, Jasdeep C Padaria","doi":"10.1007/s11248-024-00385-x","DOIUrl":"10.1007/s11248-024-00385-x","url":null,"abstract":"Δ1-pyrroline-5-carboxylate synthetase (P5CS) is one of the key regulatory enzymes involved in the proline biosynthetic pathway. Proline acts as an osmoprotectant, molecular chaperone, antioxidant, and regulator of redox homeostasis. The accumulation of proline during stress is believed to confer tolerance in plants. In this study, we cloned the complete CDS of the P5CS from pearl millet (Pennisetum glaucum (L.) R.Br. and transformed into tobacco. Three transgenic tobacco plants with single-copy insertion were analyzed for drought and heat stress tolerance. No difference was observed between transgenic and wild-type (WT) plants when both were grown in normal conditions. However, under heat and drought, transgenic plants have been found to have higher chlorophyll, relative water, and proline content, and lower malondialdehyde (MDA) levels than WT plants. The photosynthetic parameters (stomatal conductance, intracellular CO2 concentration, and transpiration rate) were also observed to be high in transgenic plants under abiotic stress conditions. qRT-PCR analysis revealed that the expression of the transgene in drought and heat conditions was 2-10 and 2-7.5 fold higher than in normal conditions, respectively. Surprisingly, only P5CS was increased under heat stress conditions, indicating the possibility of feedback inhibition. Our results demonstrate the positive role of PgP5CS in enhancing abiotic stress tolerance in tobacco, suggesting its possible use to increase abiotic stress-tolerance in crops for sustained yield under adverse climatic conditions.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140913711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coexistence field trials between MON810 and conventional maize in Mallorca as a basis for a regional regulatory proposal based on scientific evidence in the times of genome editing. 在马略卡岛进行的 MON810 和传统玉米共存田间试验，作为在基因组编辑时代根据科学证据提出地区监管建议的基础。

IF 3 3区生物学

Transgenic Research Pub Date : 2024-06-01 Epub Date: 2024-05-07 DOI: 10.1007/s11248-024-00384-y

Juan Antonio Vives-Vallés, Maria Corujo, Maria Pla, Jeroni Galmés

{"title":"Coexistence field trials between MON810 and conventional maize in Mallorca as a basis for a regional regulatory proposal based on scientific evidence in the times of genome editing.","authors":"Juan Antonio Vives-Vallés, Maria Corujo, Maria Pla, Jeroni Galmés","doi":"10.1007/s11248-024-00384-y","DOIUrl":"10.1007/s11248-024-00384-y","url":null,"abstract":"This paper reports the first coexistence field trials between transgenic and conventional maize carried out under Mediterranean island conditions. Their purpose was to assess the local validity of pollen barriers and sowing delays as coexistence strategies as a basis for a regional regulation on the subject. Two field trials were performed in two agricultural states of Alcudia and Palma, in Mallorca (Spain). In the first one, two adjacent plots were synchronously sown with conventional and transgenic maize, respectively. In the second trial, the previous design was replicated, and two additional plots sown with GM maize were added, paired with their respective conventional recipient plots sown 2 and 4 weeks later. All conventional plots were located downwind from their respective GM plots. Of the two conventional plots in sowing synchrony, only one of them required a 2.25 m pollen barrier to meet the 0.9% labeling threshold. A 4-week sowing delay between GM and non-GM plots proved to be enough to keep the GM content of the recipient plots below the legal threshold. However, with a 2-week sowing delay additional coexistence measures such as pollen barriers might be needed, as suggested in the literature. Results are consistent with previous research conducted in the northeast of Spain, thus validating in the island's agroclimatic conditions a model successfully tested in that peninsular region which allows to accurately estimate the need and width of pollen barriers. The results presented here could perhaps be extrapolated to other islands, coastal areas, and regions with stable prevailing winds during the maize flowering season.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11176244/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140864923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation and characterization of cerebellar granule neurons specific knockout mice of Golli-MBP 小脑颗粒神经元特异性Golli-MBP基因敲除小鼠的产生和特征描述

IF 3 3区生物学

Transgenic Research Pub Date : 2024-04-29 DOI: 10.1007/s11248-024-00382-0

Haruko Miyazaki, Saki Nishioka, Tomoyuki Yamanaka, Manabu Abe, Yukio Imamura, Tomohiro Miyasaka, Nobuto Kakuda, Toshitaka Oohashi, Tomomi Shimogori, Kazuhiro Yamakawa, Masahito Ikawa, Nobuyuki Nukina

{"title":"Generation and characterization of cerebellar granule neurons specific knockout mice of Golli-MBP","authors":"Haruko Miyazaki, Saki Nishioka, Tomoyuki Yamanaka, Manabu Abe, Yukio Imamura, Tomohiro Miyasaka, Nobuto Kakuda, Toshitaka Oohashi, Tomomi Shimogori, Kazuhiro Yamakawa, Masahito Ikawa, Nobuyuki Nukina","doi":"10.1007/s11248-024-00382-0","DOIUrl":"https://doi.org/10.1007/s11248-024-00382-0","url":null,"abstract":"Golli–myelin basic proteins, encoded by the myelin basic protein gene, are widely expressed in neurons and oligodendrocytes in the central nervous system. Further, prior research has shown that Golli–myelin basic protein is necessary for myelination and neuronal maturation during central nervous system development. In this study, we established Golli–myelin basic protein-floxed mice to elucidate the cell-type-specific effects of Golli–myelin basic protein knockout through the generation of conditional knockout mice (Golli–myelin basic proteinsfl/fl; E3CreN), in which Golli–myelin basic proteins were specifically deleted in cerebellar granule neurons, where Golli–myelin basic proteins are expressed abundantly in wild-type mice. To investigate the role of Golli–myelin basic proteins in cerebellar granule neurons, we further performed histopathological analyses of these mice, with results indicating no morphological changes or degeneration of the major cellular components of the cerebellum. Furthermore, behavioral analysis showed that Golli–myelin basic proteinsfl/fl; E3CreN mice were healthy and did not display any abnormal behavior. These results suggest that the loss of Golli–myelin basic proteins in cerebellar granule neurons does not lead to cerebellar perturbations or behavioral abnormalities. This mouse model could therefore be employed to analyze the effect of Golli–myelin basic protein deletion in specific cell types of the central nervous system, such as other neuronal cells and oligodendrocytes, or in lymphocytes of the immune system.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140828151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulatory landscape and public perception for gene-edited bananas in the Southeast Asian region 东南亚地区对基因编辑香蕉的监管情况和公众看法

IF 3 3区生物学

Transgenic Research Pub Date : 2024-04-10 DOI: 10.1007/s11248-024-00379-9

Nurzatil Sharleeza Mat Jalaluddin, Abdulah Al-Hadi Ahmad Fuaad, Rofina Yasmin Othman

{"title":"Regulatory landscape and public perception for gene-edited bananas in the Southeast Asian region","authors":"Nurzatil Sharleeza Mat Jalaluddin, Abdulah Al-Hadi Ahmad Fuaad, Rofina Yasmin Othman","doi":"10.1007/s11248-024-00379-9","DOIUrl":"https://doi.org/10.1007/s11248-024-00379-9","url":null,"abstract":"Banana is a premier fruit crop in many parts of the world especially Southeast Asia. The demand for banana has contributed to significant national income to primary banana producers in the SEA region such as the Philippines, Indonesia, Thailand, Vietnam, and Malaysia. However, the widely traded banana industry is plagued by numerous threats including pests and diseases, post-harvest issues and extreme climate vulnerability. To address these challenges, new breeding techniques such as gene editing have been explored for breeding programs to develop improved banana varieties. The first gene-edited non-browning banana has been deregulated in the Philippines recently, and more regulatory applications are expected to submit for approvals soon. Hence, it is timely to review the policy options for gene editing that have been adopted and discussed in the Southeast Asian countries and highlight the implications of differing regulatory approaches to gene editing for trading activities. Positive stakeholders’ perceptions and public acceptance are key factors in allowing the benefits of gene editing and thus appropriate outreach strategies are important to gain acceptance and avoid the “GMO stigma” that may be associated with gene-edited products.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140585818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prey-mediated effects of Mpp51Aa2-producing cotton on longevity and reproduction of Orius majusculus 产生 Mpp51Aa2 的棉花在猎物介导下对黄莺寿命和繁殖的影响

IF 3 3区生物学

Transgenic Research Pub Date : 2024-04-05 DOI: 10.1007/s11248-024-00378-w

Michael Meissle

{"title":"Prey-mediated effects of Mpp51Aa2-producing cotton on longevity and reproduction of Orius majusculus","authors":"Michael Meissle","doi":"10.1007/s11248-024-00378-w","DOIUrl":"https://doi.org/10.1007/s11248-024-00378-w","url":null,"abstract":"Genetically engineered (GE) cotton event MON 88702, producing Mpp51Aa2 (previously mCry51Aa2) from Bacillus thuringiensis (Bt), controls sucking pests, such as Lygus spp. (Hemiptera: Miridae) and thrips (Thysanoptera). Ingesting high doses of the insecticidal protein resulted in adverse effects on life table parameters of beneficial, predatory Orius spp. (Hemiptera: Anthocoridae). This triggered laboratory studies with more realistic food treatments, including different combinations of prey types with and without Bt protein to further characterize risks to this important group of non-target organisms. In this work, exclusive feeding of frozen spider mites (Tetranychus urticae, Acari: Tetranychidae) from Bt cotton confirmed adverse effects on longevity and fecundity of O. majusculus adults. Alternate feeding of Bt protein-containing spider mites and Bt-free Ephestia kuehniella (Lepidoptera: Pyralidae) eggs mitigated effects on longevity, but not on fecundity. When living larvae of Spodoptera littoralis (Lepidoptera: Noctuidae) from Bt cotton were fed to the predators, however, no effects on longevity and reproduction of female O. majusculus were observed, despite the fact that Bt protein concentrations in larvae were almost as high as concentrations in spider mites. When a diverse mix of prey species with various Bt protein concentrations is consumed in the field, it is unlikely that exposure of Orius spp. to Mpp51Aa2 is high enough to exert adverse effects on predator populations. MON 88702 cotton may thus be a valuable tool for integrated management of sucking pests.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140585789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Membrane-based inverse-transition purification facilitates a rapid isolation of various spider-silk elastin-like polypeptide fusion proteins from extracts of transgenic tobacco 基于膜的反转纯化技术有助于从转基因烟草提取物中快速分离各种蜘蛛丝弹性蛋白样多肽融合蛋白

IF 3 3区生物学

Transgenic Research Pub Date : 2024-04-04 DOI: 10.1007/s11248-024-00375-z

H. M. Gruchow, P. Opdensteinen, J. F. Buyel

{"title":"Membrane-based inverse-transition purification facilitates a rapid isolation of various spider-silk elastin-like polypeptide fusion proteins from extracts of transgenic tobacco","authors":"H. M. Gruchow, P. Opdensteinen, J. F. Buyel","doi":"10.1007/s11248-024-00375-z","DOIUrl":"https://doi.org/10.1007/s11248-024-00375-z","url":null,"abstract":"Plants can produce complex pharmaceutical and technical proteins. Spider silk proteins are one example of the latter and can be used, for example, as compounds for high-performance textiles or wound dressings. If genetically fused to elastin-like polypeptides (ELPs), the silk proteins can be reversibly precipitated from clarified plant extracts at moderate temperatures of ~ 30 °C together with salt concentrations > 1.5 M, which simplifies purification and thus reduces costs. However, the technologies developed around this mechanism rely on a repeated cycling between soluble and aggregated state to remove plant host cell impurities, which increase process time and buffer consumption. Additionally, ELPs are difficult to detect using conventional staining methods, which hinders the analysis of unit operation performance and process development. Here, we have first developed a surface plasmon resonance (SPR) spectroscopy-based assay to quantity ELP fusion proteins. Then we tested different filters to prepare clarified plant extract with > 50% recovery of spider silk ELP fusion proteins. Finally, we established a membrane-based purification method that does not require cycling between soluble and aggregated ELP state but operates similar to an ultrafiltration/diafiltration device. Using a data-driven design of experiments (DoE) approach to characterize the system of reversible ELP precipitation we found that membranes with pore sizes up to 1.2 µm and concentrations of 2–3 M sodium chloride facilitate step a recovery close to 100% and purities of > 90%. The system can thus be useful for the purification of ELP-tagged proteins produced in plants and other hosts.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140602069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The SpRY Cas9 variant release the PAM sequence constraint for genome editing in the model plant Physcomitrium patens SpRY Cas9 变体释放 PAM 序列约束，在模式植物 Physcomitrium patens 中进行基因组编辑

IF 3 3区生物学

Transgenic Research Pub Date : 2024-04-04 DOI: 10.1007/s11248-024-00381-1

Julie Calbry, Guillaume Goudounet, Florence Charlot, Anouchka Guyon-Debast, Pierre-François Perroud, Fabien Nogué

{"title":"The SpRY Cas9 variant release the PAM sequence constraint for genome editing in the model plant Physcomitrium patens","authors":"Julie Calbry, Guillaume Goudounet, Florence Charlot, Anouchka Guyon-Debast, Pierre-François Perroud, Fabien Nogué","doi":"10.1007/s11248-024-00381-1","DOIUrl":"https://doi.org/10.1007/s11248-024-00381-1","url":null,"abstract":"Genome editing via CRISPR/Cas has enabled targeted genetic modifications in various species, including plants. The requirement for specific protospacer-adjacent motifs (PAMs) near the target gene, as seen with Cas nucleases like SpCas9, limits its application. PAMless SpCas9 variants, designed with a relaxed PAM requirement, have widened targeting options. However, these so-call PAMless SpCas9 still show variation of editing efficiency depending on the PAM and their efficiency lags behind the native SpCas9. Here we assess the potential of a PAMless SpCas9 variant for genome editing in the model plant Physcomitrium patens. For this purpose, we developed a SpRYCas9i variant, where expression was optimized, and tested its editing efficiency using the APT as a reporter gene. We show that the near PAMless SpRYCas9i effectively recognizes specific PAMs in P. patens that are not or poorly recognized by the native SpCas9. Pattern of mutations found using the SpRYCas9i are similar to the ones found with the SpCas9 and we could not detect off-target activity for the sgRNAs tested in this study. These findings contribute to advancing versatile genome editing techniques in plants.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140586180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Specific activity and utility of recombinant cellobiohydrolase II (Cel6A) produced in maize endosperm. 玉米胚乳中产生的重组纤维生物水解酶 II (Cel6A) 的特异性活性和实用性。

IF 3 3区生物学

Transgenic Research Pub Date : 2024-04-01 Epub Date: 2024-03-07 DOI: 10.1007/s11248-024-00376-y

Enio Duque Y Duque, Milena Aguirre, Nathan C Hood, Elizabeth E Hood

{"title":"Specific activity and utility of recombinant cellobiohydrolase II (Cel6A) produced in maize endosperm.","authors":"Enio Duque Y Duque, Milena Aguirre, Nathan C Hood, Elizabeth E Hood","doi":"10.1007/s11248-024-00376-y","DOIUrl":"10.1007/s11248-024-00376-y","url":null,"abstract":"Cellobiohydrolase II (CBH II) is an exo-glucanase that is part of a fungal mixture of enzymes from a wood-rot fungus, Trichoderma reesei. It is therefore difficult to purify and to establish a specific activity assay. The gene for this enzyme, driven by the rice Os glutelin promoter, was transformed into High II tissue culture competent corn, and the enzyme accumulated in the endosperm of the seed. The transgenic line recovered from tissue culture was bred into male and female elite Stine inbred corn lines, stiff stalk 16083-025 (female) and Lancaster MSO411 (male), for future production in their hybrid. The enzyme increases its accumulation throughout its 6 generations of back crosses, 27-266-fold between T1 and T2, and 2-10-fold between T2 and T3 generations with lesser increases in T4-T6. The germplasm of the inbred lines replaces the tissue culture corn variety germplasm with each generation, with the ultimate goal of producing a high-yielding hybrid with the transgene. The CBH II enzyme was purified from T5 inbred male grain 10-fold to homogeneity with 47.5% recovery. The specific activity was determined to be 1.544 units per µg protein. The corn-derived CBH II works in biopolishing of cotton by removing surface fibers to improve dyeability and increasing glucose from corn flour for increasing ethanol yield from starch-based first-generation processes.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140050437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of a hydrodynamic delivery system in ducks. 建立鸭子水动力输送系统。

IF 3 3区生物学

Transgenic Research Pub Date : 2024-04-01 Epub Date: 2024-03-09 DOI: 10.1007/s11248-024-00377-x

Zhanji Zhao, Jiabing Zhu, Lijian Zhou, Nan Sun, Kaile Chang, Xiaoyue Hu, Yuting Hu, Mingzhi Ren, Yan Cheng, Derong Xu, Hongbo Xin, Chunbo Zhang

{"title":"Establishment of a hydrodynamic delivery system in ducks.","authors":"Zhanji Zhao, Jiabing Zhu, Lijian Zhou, Nan Sun, Kaile Chang, Xiaoyue Hu, Yuting Hu, Mingzhi Ren, Yan Cheng, Derong Xu, Hongbo Xin, Chunbo Zhang","doi":"10.1007/s11248-024-00377-x","DOIUrl":"10.1007/s11248-024-00377-x","url":null,"abstract":"Chronic hepatitis B virus (HBV) poses a significant global health challenge as it can lead to acute or chronic liver disease and hepatocellular carcinoma (HCC). To establish a safety experimental model, a homolog of HBV-duck HBV (DHBV) is often used for HBV research. Hydrodynamic-based gene delivery (HGD) is an efficient method to introduce exogenous genes into the liver, making it suitable for basic research. In this study, a duck HGD system was first constructed by injecting the reporter plasmid pLIVE-SEAP via the ankle vein. The highest expression of SEAP occurred when ducks were injected with 5 µg/mL plasmid pLIVE-SEAP in 10% bodyweight volume of physiological saline for 6 s. To verify the distribution and expression of exogenous genes in multiple tissues, the relative level of foreign gene DNA and β-galactosidase staining of LacZ were evaluated, which showed the plasmids and their products were located mainly in the liver. Additionally, β-galactosidase staining and fluorescence imaging indicated the delivered exogenous genes could be expressed in a short time. Further, the application of the duck HGD model on DHBV treatment was investigated by transferring representative anti-HBV genes IFNα and IFNγ into DHBV-infected ducks. Delivery of plasmids expressing IFNα and IFNγ inhibited DHBV infection and we established a novel efficient HGD method in ducks, which could be useful for drug screening of new genes, mRNAs and proteins for anti-HBV treatment.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140068757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0