Tohoku Journal of Experimental Medicine最新文献_第2页

Tubacin Decelerates TGF-β1-Induced Pro-Fibrotic Effects on Human Dermal Fibroblasts by Regulating HDAC6-Mediated Deacetylation of α-Tubulin and Hsp90.

IF 1.7 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-03-20 DOI: 10.1620/tjem.2025.J040

Yanli Wang, Liu Yang, Tao Wang, Xiuli Li, Xin Zhang

引用次数: 0

Prognostic Value of Systemic Immune-Inflammation Index and Systemic Inflammatory Response Index on Functional Status and Mortality in Patients with Critical Acute Ischemic Stroke. 全身免疫炎症指数和全身炎症反应指数对危重急性缺血性脑卒中患者功能状态和死亡率的预后价值

IF 1.7 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-03-15 Epub Date: 2024-09-12 DOI: 10.1620/tjem.2024.J094

Kadir Arslan, Ayca Sultan Sahin

{"title":"Prognostic Value of Systemic Immune-Inflammation Index and Systemic Inflammatory Response Index on Functional Status and Mortality in Patients with Critical Acute Ischemic Stroke.","authors":"Kadir Arslan, Ayca Sultan Sahin","doi":"10.1620/tjem.2024.J094","DOIUrl":"10.1620/tjem.2024.J094","url":null,"abstract":"Neuroinflammation plays an essential role in the pathogenesis of acute ischemic stroke (AIS). This study aims to investigate the predictive value of the systemic immune-inflammation index (SII) and systemic inflammatory response index (SIRI) on mortality and functional limitation in patients with critical AIS. Patients with critical AIS in a tertiary hospital's intensive care unit (ICU) between June 2020 and 2022 were retrospectively examined. Patients were classified according to their 28-day mortality (survivor and non-survivor group) and functional status (poor and good functional outcomes). The performances of SII and SIRI in predicting mortality and functional outcomes were compared. A total of 198 patients were included in the study. The median age of the entire population was 70 (56-86) years, and 52% (n = 103) were male. Coronary vascular disease/heart failure was found to be significantly higher in the mortality group (p = 0.025). While SII was found to be significantly higher in the mortality group (1,180 vs. 811, p = 0.038), SIRI did not show a significant difference (1.82 vs. 1.70, p = 0.257). SII and SIRI were significantly higher in the poor functional outcome group (p < 0.001 and p = 0.015). In the ROC analysis of the functional status prediction performances of SII and SIRI, the cut-off value of SII was ≥ 1,146, the area under the curve (AUC) = 0.645 (0.568-0.722), the cut-off value of SIRI was ≤ 2.54, AUC = 0.600 (0.520-0.680) was detected. SII helps predict 28-day mortality in patients with critical AIS. Both SII and SIRI can predict functional status at discharge.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"91-97"},"PeriodicalIF":1.7,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Molecular Mechanism by which LINC00461 Regulates Carfilzomib Resistance in Multiple Myeloma. LINC00461调控多发性骨髓瘤卡非佐米耐药性的分子机制

IF 1.7 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-03-15 Epub Date: 2024-09-05 DOI: 10.1620/tjem.2024.J082

Lifang Cheng, Fanjuan Zhang

{"title":"The Molecular Mechanism by which LINC00461 Regulates Carfilzomib Resistance in Multiple Myeloma.","authors":"Lifang Cheng, Fanjuan Zhang","doi":"10.1620/tjem.2024.J082","DOIUrl":"10.1620/tjem.2024.J082","url":null,"abstract":"Multiple myeloma (MM) is a frequent haematological malignancy of the bone marrow. Carfilzomib, a first-line treatment for MM, has excellent antitumour effects, but its efficacy eventually decreases due to primary or acquired chemoresistance. Therefore, the regulatory mechanism of carfilzomib resistance has attracted much attention for improving the survival outcomes of patients with MM. By using database analysis combined with quantitative real-time polymerase chain reaction (qRT‒PCR), aberrant lncRNAs in MM were screened in carfilzomib-resistant cells versus carfilzomib-sensitive cells, and the resistance index of cultured carfilzomib-resistant cells was analysed compared to that of parental cells. Furthermore, cell viability, proliferation, and apoptosis in response to treatment with carfilzomib were measured after treatment with LINC00461 by the cell counting kit-8 (CCK-8) method and flow cytometry. The expression of LINC00461 was also verified through qRT‒PCR. Then, the possible miRNA molecules on which LINC00461 may act were investigated by RNA immunoprecipitation (RIP) and dual-luciferase reporter assays. Next, tumorigenesis in mice was evaluated to verify the effect of LINC00461 on carfilzomib-resistant cells. Increased expression of LINC00461 was related to drug resistance in MM patients. Mechanistically, LINC00461 overexpression attenuated the effect of miR-539-3p overexpression and decreased the expression of the downstream protein RAB5A. Moreover, compared with the control group, the LINC00461 knockdown group treated with carfilzomib exhibited decreases in tumour volume and weight. Furthermore, LINC00461 sensitized carfilzomib-sensitive cells by promoting the release of exosomes. These data suggest that LINC00461 plays an important role in the development of carfilzomib resistance.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"99-111"},"PeriodicalIF":1.7,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Novel Potential of Serum SIRT1 and TIMP3 as Biomarkers for In-Stent Restenosis Following Percutaneous Transluminal Angioplasty and Stenting in Arteriosclerosis Obliterans Patients. 探索血清 SIRT1 和 TIMP3 作为动脉硬化闭塞症患者经皮腔内血管成形术和支架置入术后支架内再狭窄生物标志物的新潜力。

IF 1.7 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-03-14 Epub Date: 2024-09-05 DOI: 10.1620/tjem.2024.J083

Han-Jun Wang, Sheng-Yuan Mao

{"title":"Exploring the Novel Potential of Serum SIRT1 and TIMP3 as Biomarkers for In-Stent Restenosis Following Percutaneous Transluminal Angioplasty and Stenting in Arteriosclerosis Obliterans Patients.","authors":"Han-Jun Wang, Sheng-Yuan Mao","doi":"10.1620/tjem.2024.J083","DOIUrl":"10.1620/tjem.2024.J083","url":null,"abstract":"This study aimed to investigate the potential of serum Sirtuin 1 (SIRT1) and tissue inhibitor of metalloproteinase 3 (TIMP3) levels as biomarkers for in-stent restenosis (ISR) in arteriosclerosis obliterans (ASO) patients following percutaneous transluminal angioplasty (PTA) and stenting. A total of 256 ASO patients who underwent successful PTA with stent implantation were included. Serum levels of SIRT1 and TIMP3 were assessed at baseline and 4 weeks post-procedure. After 6 months, 65 patients were identified with ISR. Significant differences were noted in serum SIRT1 and TIMP3 levels between ISR and non-ISR groups at 4 weeks. TIMP3 had a higher AUC (0.782, 95% CI: 0.726-0.831) than SIRT1 (0.737, 95% CI: 0.678-0.789) for predicting ISR at 6 months. Correlation analysis showed a positive association between SIRT1 and TIMP3 levels in ISR patients at 4 weeks, but not in non-ISR patients. Multivariate analysis revealed diabetes (OR = 1.436, 95% CI: 1.205-1.925) and carotid stenosis (OR = 4.551, 95% CI: 1.364-15.185) significantly increased ISR risk, while lower SIRT1 (OR = 0.985, 95% CI: 0.978-0.992) and TIMP3 (OR = 0.574, 95% CI: 0.464-0.710) levels were significantly associated with ISR. Serum SIRT1 and TIMP3 levels at 4 weeks post-procedure are significant predictors of ISR in ASO patients following PTA and stenting. Lower SIRT1 and TIMP3 levels correlate with higher ISR risk. These findings suggest that monitoring serum SIRT1 and TIMP3 levels could be a valuable tool in predicting ISR, which could inform clinical decisions and patient management.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"83-90"},"PeriodicalIF":1.7,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long Noncoding LINC00115 Facilitates Cell Growth and Inhibits Apoptosis by Regulating the miR-4701-5p/P4HB Axis in Bladder Cancer. 长非编码 LINC00115 通过调控 MiR-4701-5p/P4HB 轴促进膀胱癌细胞生长并抑制凋亡

IF 1.7 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-03-14 Epub Date: 2024-08-08 DOI: 10.1620/tjem.2024.J075

Changyuan Dai, Qingwen Li, Lili Wang, Jiajun Zhang, Shuai Yang, Xiaole Zhang

{"title":"Long Noncoding LINC00115 Facilitates Cell Growth and Inhibits Apoptosis by Regulating the miR-4701-5p/P4HB Axis in Bladder Cancer.","authors":"Changyuan Dai, Qingwen Li, Lili Wang, Jiajun Zhang, Shuai Yang, Xiaole Zhang","doi":"10.1620/tjem.2024.J075","DOIUrl":"10.1620/tjem.2024.J075","url":null,"abstract":"Bladder cancer (BCa) is a prevalent urogenital malignancy, imposing a significant burden on health-care systems worldwide. Long noncoding RNAs (lncRNAs) are important regulators of carcinogenesis and affect BCa progression. In this study, the influence of lncRNA LINC00115 on malignant behavior of BCa cells were explored. Bioinformatics method was used for prediction of gene expression and downstream molecules of LIN00115. LINC00115 expression level in BCa cells was measured using RT-qPCR. After LINC00115 depletion, the proportion of viable, proliferative, and apoptotic BCa cells were calculated by methyl thiazolyl tetrazolium (MTT) assays, colony formation assays, and TUNEL staining, respectively. FISH was performed to verify the cellular distribution of LINC00115. The interaction between LINC00115 and miR-4701-5p and the binding between miR-4701-5p and P4HB were confirmed using RNA pulldown, RNA immunoprecipitation (RIP), and luciferase reporter assays. Experimental results showed that LINC00115 was highly expressed in BCa cells. The silencing of LINC00115 restrained BCa cell proliferation and stimulated apoptosis. LINC00115 could directly bind to miR-4701-5p and thus initiate P4HB upregulation in BCa cells. P4HB 3'untranslated region could be targeted by miR-4701-5p. Additionally, Amplification of P4HB expression offset the effects of LINC00115 knockdown on BCa cell proliferative and apoptotic behaviors. In conclusion, LINC00115 facilitates BCa cell growth and inhibits apoptosis via interaction with miR-4701-5p and upregulation of P4HB.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"69-81"},"PeriodicalIF":1.7,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141902954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wnt2 Expression in Cancer-Associated Fibroblasts Precedes Lymph Node Metastasis in Orthotopic Transplantation Mouse Model of Colorectal Cancer.

IF 1.7 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-03-13 DOI: 10.1620/tjem.2025.J039

Hideaki Karasawa, Keigo Murakami, Koh Miura, Hiroyuki Sasaki, Tomoyuki Ono, Taiki Kajiwara, Akihiro Yamamura, Takashi Kamei, Shinobu Ohnuma, Toru Furukawa, Michiaki Unno

引用次数: 0

A Review on the Potential Therapeutic Application of Macrophage Polarization in Recurrent Spontaneous Abortion; With an Emphasis on Natural Components. 关于巨噬细胞极化在复发性自然流产中的潜在治疗应用的综述；重点是天然成分。

IF 1.7 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-03-13 Epub Date: 2024-07-25 DOI: 10.1620/tjem.2024.J069

Tianqi Qiao, Ningning Liu, Yijian Cui, Yanxia Gao, Jing Lu

{"title":"A Review on the Potential Therapeutic Application of Macrophage Polarization in Recurrent Spontaneous Abortion; With an Emphasis on Natural Components.","authors":"Tianqi Qiao, Ningning Liu, Yijian Cui, Yanxia Gao, Jing Lu","doi":"10.1620/tjem.2024.J069","DOIUrl":"10.1620/tjem.2024.J069","url":null,"abstract":"Three or more consecutive spontaneous pregnancy losses are the hallmark of recurrent spontaneous abortion (RSA) as a complex challenge in reproductive health, requiring a comprehensive understanding of contributing factors. Since balanced immune responses are essential for a successful pregnancy. Disruptions in immune responses may be the cause of unfavorable pregnancy outcomes like RSA. Of Note, Following RSA, immunopathological assessment of the placental implantation site markedly showed decidual inflammation, leading to hypothesize that RSA is a pregnancy disorder with an inflammatory etiology. Thus, an in-depth knowledge of how immune cells contribute to inflammation, may lead to the discovery of the novel therapeutic approaches for the prevention and/or treatment of RSA. Numerous studies have investigated the relationships between RSA and different immune cells, including B cells, T cells, decidual dendritic cells, and macrophages. Macrophages are present at the fetomaternal interface throughout pregnancy, and they are beneficial to the processes of embryonic development, placental formation, embryo implantation, and delivery. Macrophages classified as typically activated (M1, with the inflammatory role), or alternatively activated (M2, characterized by the anti-inflammatory role). Plants have a rich supply of strong bioactive components that can polarize macrophages toward an M1 pro-inflammatory state or an M2 anti-inflammatory phenotype. This review focuses on the potential role of derived plant-natural components in influencing macrophage polarization resulting in the management treatment of RSA.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"47-58"},"PeriodicalIF":1.7,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141761070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LncRNA-CR848007.6 Affects Insulin Secretion by Regulating Cell Apoptosis and DNA Damage in Cadmium-Exposed Pancreatic β Cells and is a Novel Biomarker in Patients with Diabetes. LncRNA-CR848007.6通过调节镉暴露的胰腺β细胞的细胞凋亡和DNA损伤影响胰岛素分泌，是糖尿病患者的一种新型生物标记物

IF 1.7 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-03-13 DOI: 10.1620/tjem.2025.J038

Zhiheng Zhou, Shengqing Zhou, Hongda Guo

引用次数: 0

Impacts of High-Intensity One-Year Respiratory Muscle Exercise on Respiratory Muscle Force and Dyspnea at Various Body Trunk Angles in a Tetraplegic Patient.

IF 1.7 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-03-13 DOI: 10.1620/tjem.2025.J037

Tatsuma Okazaki, Ryo Takahashi, Taruhi Matsui, Midori Miyagi, Takahiro Miura, Naoki Suzuki, Satoru Ebihara

引用次数: 0

Practical Coagulation Management in Liver Transplantation Through Point-of-Care Analysis Using the TEG 6s Global Hemostasis System in Japan. 在日本，通过使用 TEG 6s 全球止血系统进行护理点分析，对肝移植中的凝血问题进行实用管理。

IF 1.7 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-03-12 Epub Date: 2024-09-05 DOI: 10.1620/tjem.2024.J087

Muneyuki Matsumura, Kengo Sasaki, Kazuaki Tokodai, Koji Miyazawa, Atsushi Fujio, Hiroyuki Ogasawara, Michiaki Unno, Takashi Kamei

{"title":"Practical Coagulation Management in Liver Transplantation Through Point-of-Care Analysis Using the TEG 6s Global Hemostasis System in Japan.","authors":"Muneyuki Matsumura, Kengo Sasaki, Kazuaki Tokodai, Koji Miyazawa, Atsushi Fujio, Hiroyuki Ogasawara, Michiaki Unno, Takashi Kamei","doi":"10.1620/tjem.2024.J087","DOIUrl":"10.1620/tjem.2024.J087","url":null,"abstract":"Liver transplantation (LT) is the standard treatment for end-stage liver disease. However, owing to a precarious balance between pro- and anticoagulation factors, patients undergoing LT are at high risk of massive bleeding and vascular thromboembolic complications. Thromboelastography (TEG) allows for the rapid, comprehensive, and accurate identification of coagulation monitoring undergoing LT. Newly released TEG 6s global hemostasis systems have been introduced, which we hypothesized could contribute to practical coagulation management in LT. TEG 6s was used for 15 patients undergoing LT at eight preset times during and after LT. Anesthesiologists and a surgical intensive care team managed coagulation during and after LT, based fully on TEG 6s findings. We focused on the citrated kaolin reaction time, citrated kaolin maximum amplitude, and functional fibrinogen maximum amplitude. TEG 6s was also used to determine transfusion principles with a focus on the details of cases with difficult to manage coagulation. Among 15 LT patients, six had massive bleeding-related complications and vascular thromboembolic complications. Case management and detailed TEG 6s results were reviewed. We recommend using the TEG 6s to obtain a comprehensive understanding of coagulation management as this global hemostasis system offers superior insights compared with standard laboratory tests.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"59-67"},"PeriodicalIF":1.7,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0