Tohoku Journal of Experimental Medicine最新文献_第2页

SPARCL1 Regulates Proliferation and Angiogenesis of HUVECs Through the DLL4/NOTCH1 Pathway in Preeclampsia. SPARCL1通过DLL4/NOTCH1通路调控子痫前期HUVECs的增殖和血管生成

IF 1.7 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-07-17 DOI: 10.1620/tjem.2025.J092

Lingfang Liu, Yan Gao, Li Ao, Chao Yang, Wanyu Zhao, Chunmei Deng, Qian Zhu, Yan Chen, Qiuli Lan, Qian Hao, Yang Wang

引用次数: 0

Association Between Weekend Catch-Up Sleep and Perceived Stress Among Adolescents in the Republic of Korea: A Nationwide Cross-Sectional Study. 大韩民国青少年周末补觉与感知压力之间的关系：全国横断面研究》。

IF 1.7 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-07-15 Epub Date: 2024-09-12 DOI: 10.1620/tjem.2024.J097

Hoon Jo, Joo Ho Cheong, Mun Sik Chung, Dong Yu Kim, Ju In Kim, Sung-Kyung Kim

{"title":"Association Between Weekend Catch-Up Sleep and Perceived Stress Among Adolescents in the Republic of Korea: A Nationwide Cross-Sectional Study.","authors":"Hoon Jo, Joo Ho Cheong, Mun Sik Chung, Dong Yu Kim, Ju In Kim, Sung-Kyung Kim","doi":"10.1620/tjem.2024.J097","DOIUrl":"10.1620/tjem.2024.J097","url":null,"abstract":"Insufficient sleep is common among adolescents in the Republic of Korea because of competitive academic environments. Many students compensate for lack of sleep through weekend catch-up sleep (CUS), however the degree of effectiveness is unclear. This study aimed to determine the association between weekend CUS and perceived stress in a large, school-based Korean adolescent sample. Data from the Korean Youth Risk Behavior Web-based Survey 2020 (KYRBWS) was used. The participants included 46,187 middle and high school students in the Republic of Korea who participated in self-administered questionnaire surveys. Weekend CUS was divided into four categories: 0 h, 0-1 h, 1-2 h, and ≥ 2 h. Odds ratios (ORs) with 95% confidence intervals (CIs) were derived using logistic regression analyses. We found that the risk of perceived stress decreased significantly in weekend CUS groups (0 < CUS < 1 [OR]: 0.879, [95% CI: 0.808-0.957]; 1 ≤ CUS < 2 [OR]: 0.852, [95% CI: 0.785-0.924]; 2 ≥ CUS [OR]: 0.836, [95% CI: 0.775-0.902]) compared to the non-CUS group after adjusting for several confounding variables. Furthermore, the higher CUS group tended to have a lower risk of perceived stress. However, in the Grade 12 participants, in both the unadjusted and adjusted models, the weekend CUS groups did not show a significant association with the risk of perceived stress. Our study showed that weekend CUS is associated with a low risk of perceived stress among Korean adolescents.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"265-272"},"PeriodicalIF":1.7,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The ZIC2-Claudin-18.2 Axis Stimulates Pancreatic Cancer Progression and Metastasis via Activation of the ERK1/2 Signaling Pathway. ZIC2-Claudin-18.2轴通过激活ERK1/2信号通路刺激胰腺癌进展和转移

IF 1.7 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-07-11 Epub Date: 2024-10-31 DOI: 10.1620/tjem.2024.J114

Xiaoping Zhou, Lanying Zou, Jun Xu, Huichuan Zhao

{"title":"The ZIC2-Claudin-18.2 Axis Stimulates Pancreatic Cancer Progression and Metastasis via Activation of the ERK1/2 Signaling Pathway.","authors":"Xiaoping Zhou, Lanying Zou, Jun Xu, Huichuan Zhao","doi":"10.1620/tjem.2024.J114","DOIUrl":"10.1620/tjem.2024.J114","url":null,"abstract":"Claudin-18.2 is considered a promising target in cancer treatment. However, studies on the role of Claudin-18.2 in pancreatic cancer are scarce, and a systematic exploration of its mechanisms of action in disease progression is missing. This research sought to reveal the detailed mechanisms by Claudin-18.2 impacts pancreatic cancer development and metastasis. Claudin-18.2 expression levels in pancreatic cancer were investigated through The Cancer Genome Atlas (TCGA) database, with subsequent validation by immunohistochemistry and quantitative reverse transcription polymerase chain reaction analysis. The potential regulatory transcription factors (TFs) for Claudin-18 were forecasted by the KnockTF database. Pearson's correlation was applied to ascertain the correlation between Claudin-18 and ZIC2, and the Molotool was utilized to analyze their potential binding sites. The TCGA database facilitated our analysis of ZIC2 expression levels within pancreatic cancer. Activation of the ERK signaling pathway was validated by western blot (WB). The colony formation assay evaluated cell proliferation, while the cell scratch and Transwell assays were used to determine cell migration and invasion abilities. WB was used to detect the expression of E-cadherin, N-cadherin and Vimentin associated with epithelial-mesenchymal transition. The ZIC2-Claudin-18.2 regulatory axis, via ERK1/2 signaling pathway activation, enhanced the malignant behaviors of pancreatic cancer. Claudin-18.2, when highly expressed in pancreatic cancer, facilitated tumor malignancy, primarily through activating the ERK1/2 signaling pathway. Additionally, ZIC2 was identified as an upstream regulatory molecule for Claudin-18.2. Our findings reveal that ZIC2, a TF, can upregulate Claudin-18.2, and initiate the ERK1/2 signaling pathway, eventually facilitating the malignant progression of pancreatic cancer.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"247-256"},"PeriodicalIF":1.7,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142547647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Neurotrophic Tyrosine Kinase Receptor Type I NTRK1 Polymorphism on Epidural Analgesia with Hydromorphone during Labor. 神经营养酪氨酸激酶受体 I 型 NTRK1 多态性对分娩期间使用氢吗啡酮硬膜外镇痛的影响

IF 1.7 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-07-11 Epub Date: 2024-11-21 DOI: 10.1620/tjem.2024.J135

Kangli Lu, Yujiao Huang, Zhicai Chen, Kuncheng Cai

{"title":"Effects of Neurotrophic Tyrosine Kinase Receptor Type I NTRK1 Polymorphism on Epidural Analgesia with Hydromorphone during Labor.","authors":"Kangli Lu, Yujiao Huang, Zhicai Chen, Kuncheng Cai","doi":"10.1620/tjem.2024.J135","DOIUrl":"10.1620/tjem.2024.J135","url":null,"abstract":"Epidural labor analgesia is a widely employed method of analgesia in current clinical practice. This study aimed to investigate the relationship between NTRK1 gene polymorphism and epidural labor analgesia, offering a novel perspective for labor analgesia related research. Epidural labor analgesia was administered with hydromorphone in 208 eligible pregnant women. Genomic DNA was extracted from EDTA anticoagulant blood samples. The NTRK1 single nucleotide polymorphisms (SNPs) rs1800880 (T > C) and rs6334 (G > A) were genotyped utilizing PCR-RFLP. Parameters such as the 24 h visual analogue score (VAS), modified Bromage score, 48 h patient controlled intravenous analgesia (PCIA) compression counts, dosage of anesthetic, adverse reactions were meticulously recorded. The immune functions were assessed by flow cytometry, and oxidative stress levels were measured by radioimmunoassay. The findings indicated that NTRK1 rs1800880 T > C and rs6334 G > A were similar, 24 h VAS score and anesthetic dosage were decreased. rs1800880 C allele was no significant difference in adverse reactions, but the level of oxidative stress was significantly reduced. The nausea and emesis response of rs6334 mutant AA was significantly reduced. Both rs1800880 TC + CC vs. TT and rs6334 GA + AA vs. GG showed significant effects on 24 h VAS score. Mutations in rs1800880 and rs6334 reduce postoperative pain, adverse reactions, and oxidative stress responses. The NTRK1 SNP may play an important role in labor analgesia and may be an effective predictor of individual pain perception, thereby providing patients with a more personalized approach to labor analgesia.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"257-264"},"PeriodicalIF":1.7,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effects of Presurgical Infant Orthopedic Treatment on the Maxillary Arch, Craniofacial Form, and Lip Morphology of Patients with Unilateral Cleft Lip and Palate, Aged up to 5 Years. 术前婴儿整形治疗对5岁以下单侧唇腭裂患者上颌弓、颅面形态和唇部形态的影响

IF 1.7 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-07-10 DOI: 10.1620/tjem.2025.J090

Mika Unno, Shinobu Tsuchiya, Chieko Miura, Akimitsu Sato, Yoshimichi Imai, Kaoru Igarashi

引用次数: 0

Association between Iron Intake and Mental Health in Older Adults: The Yamagata Cohort Study. 老年人铁摄入量与心理健康之间的关系：山形队列研究

IF 1.7 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-07-10 DOI: 10.1620/tjem.2025.J089

Yuuki Tanaka, Tsuneo Konta, Haruka Ono, Yoshiyuki Ueno, Kaori Sakurada

引用次数: 0

Long Non-coding RNA RASSF8-AS1 Promotes M1 Macrophage Polarization in Osteoarthritis via Moderating miR-27a-3p. 长非编码 RNA RASSF8-AS1 通过调节 miR-27a-3p 促进骨关节炎中 M1 型巨噬细胞的极化

IF 1.7 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-07-09 Epub Date: 2024-09-12 DOI: 10.1620/tjem.2024.J092

Haijun Shi, Meizhi Liu, Wenhan Ma, Zhigang Chen, Yongyun Shi

{"title":"Long Non-coding RNA RASSF8-AS1 Promotes M1 Macrophage Polarization in Osteoarthritis via Moderating miR-27a-3p.","authors":"Haijun Shi, Meizhi Liu, Wenhan Ma, Zhigang Chen, Yongyun Shi","doi":"10.1620/tjem.2024.J092","DOIUrl":"10.1620/tjem.2024.J092","url":null,"abstract":"Long non-coding RNAs are major regulators in the pathophysiology of osteoarthritis (OA), which involves the dysfunction of cartilage and synovium. The aim of this study was, therefore, to discover the role of RASSF8-AS1 in cartilage degradation and M1 macrophage polarization during OA. Healthy and OA cartilage and synovium were collected. After measuring RASSF8-AS1 levels in tissues and lipopolysaccharide (LPS)- or IL-1β-induced cells, the role of RASSF8-AS1 in the expression of cartilage degradation markers and M1 macrophage molecules was assessed in vitro. The apoptotic rate of IL-1β-stimulated chondrocytes, with or without RASSF8-AS1 overexpression, was quantified using flow cytometry. RASSF8-AS1 was signiﬁcantly upregulated not only in cartilage and synovium from OA patients, but also in IL-1β- or LPS-induced cells, compared to normal controls. A decrease in RASSF8-AS1 level increased the expression of chondrogenic markers, but reduced the expression of genes encoding matrix-degrading proteases, thereby reducing cell apoptosis. Downregulation of RASSF8-AS1 reduced the M1 macrophage markers in RAW264.7 cells and bone marrow-derived macrophages. RASSF8-AS1 may be a ceRNA for miR-27a-3p. These ﬁndings support the role of RASSF8-AS1 as a promoting factor of cartilage degradation and M1 macrophage polarization in OA.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"219-227"},"PeriodicalIF":1.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of the lncRNA HOTAIR Gene rs4759314 with Susceptibility to Recurrent Spontaneous Abortion in a Chinese Han Population. 中国汉族人群中 lncRNA HOTAIR 基因 rs4759314 与复发性流产易感性的关系

IF 1.7 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-07-09 Epub Date: 2024-09-05 DOI: 10.1620/tjem.2024.J084

Binglai Luo, Xiaoli Liu, Jiangang Huang, Jinyong Wang

{"title":"Association of the lncRNA HOTAIR Gene rs4759314 with Susceptibility to Recurrent Spontaneous Abortion in a Chinese Han Population.","authors":"Binglai Luo, Xiaoli Liu, Jiangang Huang, Jinyong Wang","doi":"10.1620/tjem.2024.J084","DOIUrl":"10.1620/tjem.2024.J084","url":null,"abstract":"This study was to examine the association between the long noncoding RNA (lncRNA) HOTAIR polymorphism (rs4759314, rs4759314) and the risk of recurrent spontaneous abortion (RSA) in Chinese Han women. 378 women with RSA and 361 pregnant women with normal pregnancies were selected as study subjects. The expression level of lncRNA HOTAIR was detected by fluorescence quantitative PCR. PCR restriction fragment length polymorphism (PCR-RFLP) method was used to detect genotyping. Logistic regression analysis was used to assess the independent factors of HOTAIR rs4759314 affecting recurrent miscarriage. The lncRNA HOTAIR gene expression level was significantly reduced in RSA patients. Rs1899663 polymorphism and rs4759314 GG genotype do not significantly increase the risk of developing RSA (P > 0.050). Rs4759314 AG genotype and rs4759314G allele significantly increased the risk of developing RSA (P < 0.050). Logistic analysis showed a significant correlation between rs4759314 and the occurrence of RSA (OR = 0.367, 95% CI = 0.232-0.580, P < 0.050). The lncRNA HOTAIR rs4759314 locus may serve as a biomarker for RSA susceptibility in the Chinese Han population.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"229-235"},"PeriodicalIF":1.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deubiquitinating Enzyme MINDY1 Facilitates Immune Escape in Breast Cancer by Maintaining the Stability of Immune Checkpoint Protein PD-L1. 去泛素化酶 MINDY1 通过维持免疫检查点蛋白 PD-L1 的稳定性促进乳腺癌的免疫逃逸

IF 1.7 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-07-08 Epub Date: 2024-10-24 DOI: 10.1620/tjem.2024.J111

Liang Ren, Li Wang, Zhewei Cao, Xuelin Yi, Yiran Chen, Yang Yang, Ya Liu

{"title":"Deubiquitinating Enzyme MINDY1 Facilitates Immune Escape in Breast Cancer by Maintaining the Stability of Immune Checkpoint Protein PD-L1.","authors":"Liang Ren, Li Wang, Zhewei Cao, Xuelin Yi, Yiran Chen, Yang Yang, Ya Liu","doi":"10.1620/tjem.2024.J111","DOIUrl":"10.1620/tjem.2024.J111","url":null,"abstract":"Featured with frequent recurrence and distant metastasis, the mortality of breast cancer (BC) increased year by year in recent decades. MINDY lysine 48 deubiquitinase 1 (MINDY1) played an oncogenic role in several tumors. The role of MINDY1 deserved further study in BC. A wide ranges of assays including qRT-PCR, Western blotting, CCK-8 flow cytometry analysis, co-immunoprecipitation assay, Pearson's coefficient tests, and tumor xenograft assay were designed and carried out to determine the role of MINDY1 in BC. Both MINDY1 and programmed death-ligand 1 (PD-L1) is upregulated in BC tissues and cells. In addition, knockdown of MINDY1 attenuated cell viability and promoted the activation of T cells. Mechanistically, MINDY1 stabilized PD-L1 protein by interacting with PD-L1 in BC cells, and MINDY1 is positively associated with PD-L1 in BC tissues. Moreover, rescue assay revealed that the effect of MINDY1 silencing on cell viability and T cell activation was reversed by PD-L1 overexpression. The in vivo study also demonstrated that the effect of MINDY1 knockdown on tumor growth induced by was counteracted by PD-L1 overexpression.In conclusion, we identified PD-L1 as a novel target of MINDY1 and established a significant association between MINDY1 and the cancer immune response. Importantly, our findings reveal that MINDY1 promoted BC progression via PD-L1-mediated immune evasion.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"237-246"},"PeriodicalIF":1.7,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142508642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KLF16-Induced Increase of GARS Promotes Glycolysis in Hepatocellular Carcinoma by Stabilizing FOXK1. klf16诱导的GARS升高通过稳定FOXK1促进肝细胞癌的糖酵解。

IF 1.7 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-07-03 DOI: 10.1620/tjem.2025.J087

Jinghui Wang, Ruijuan Fan, Jun Li, Hanbo Qiu, Lizhu Lin

引用次数: 0