Transactions of the ASABE最新文献

{"title":"Techno-Economic Analysis of Constant-Flow Woodchip Bioreactors","authors":"Lindsey M. Hartfiel, M. Soupir, K. Rosentrater","doi":"10.13031/TRANS.14300","DOIUrl":"https://doi.org/10.13031/TRANS.14300","url":null,"abstract":"HighlightsTechno-economic analysis was performed for multiple scales of bioreactors operated under a variety of conditions.The unit cost decreased as the bioreactor size increased.The unit cost increased in bioreactors with longer HRTs and bypass flow due to reduced treatment capacity.One large bioreactor was more cost-effective than multiple smaller bioreactors.Abstract. Woodchip denitrification bioreactors are a relatively new, edge-of-field technology used to reduce nitrate-nitrogen (NO3-N) from subsurface tile drainage. The removal rate of nitrate is influenced by many factors, including temperature, dissolved oxygen, and hydraulic residence time (HRT). The objective of this study was to conduct a techno-economic analysis (TEA) for four scales of woodchip denitrification bioreactors operating at three HRTs (2, 8, and 16 h), designed with bypass flow or with a low probability of bypass flow, to determine the cost to remove 1 kg of NO3-N at each bioreactor scale and at each HRT. Several assumptions were made: the flow rate required to achieve a 2 h HRT on a per m3 basis could be achieved at all scales, the same mass removal of NO3-N was achieved on a per cubic meter basis, and the 2 h HRT did not have any bypass flow at each scale. With these assumptions, the lowest unit cost was observed for the large-scale bioreactor sized to have a low probability of bypass flow at 16 h HRT, with a resulting cost of $0.74 kg-1 NO3-N removed. The highest unit cost was observed for the pilot-scale bioreactor designed with bypass flow to achieve a 16 h HRT at a cost of $60.13 kg-1 NO3-N removed. At longer HRTs with bypass flow, a greater percent removal of nitrate has been observed with a lower mass removal rate. By having a low probability of bypass flow in the design, a higher mass removal and percent removal of nitrate were observed, leading to the above results. Contrasting this trend, the total and annual costs were highest for the large-scale bioreactor and lowest for the pilot-scale bioreactor. However, it was determined that 783%, 280%, and 54% increases in total cost for the pilot-, small-, and medium-scale bioreactors would be incurred to implement the number of bioreactors (66, 24, and 4, respectively) required to treat the same volume of flow as one large bioreactor. These results can be used to inform future design decisions and inform stakeholders of the approximate unit cost of installing a denitrifying woodchip bioreactor over a range of expected field conditions. While a larger bioreactor with a low probability of bypass flow may represent a more cost-effective investment, the potential for unintended, negative byproducts needs to be considered in the design. Keywords: Denitrification, Nitrate, Tile drainage, Water quality, Woodchip bioreactor.","PeriodicalId":23120,"journal":{"name":"Transactions of the ASABE","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81079831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical Concentration and Spatial Uniformity of a Premixing In-Line Injection System Attached to a Variable-Rate Orchard Sprayer","authors":"Zhihong Zhang, Heping Zhu, Zhiming Wei, R. Salcedo","doi":"10.13031/trans.14113","DOIUrl":"https://doi.org/10.13031/trans.14113","url":null,"abstract":"HighlightsA newly developed premixing in-line injection system attached to a variable-rate orchard sprayer was evaluated.Tests were conducted to verify the in-line injection system performance using a vertical spray patternator.Concentration accuracy and spatial distribution uniformity were determined with a fluorescent tracer.Uniform spray mixtures were obtained for different spray viscosities and duty cycle combinations.Abstract. Pesticide spray application efficiency is highly dependent on the chemical concentration accuracy and spatial distribution uniformity. In this study, the performance of a newly developed premixing in-line injection system was evaluated when it was attached to a laser-guided, pulse width modulated (PWM), variable-rate orchard sprayer. The chemical concentration accuracy was determined with respect to spray deposition with a fluorescent tracer, and the spatial distribution uniformity was determined with spray deposits at different heights on a vertical spray patternator. Outdoor tests were conducted with 27 combinations of target chemical concentration (1.0%, 1.5%, and 2.0%), viscosity of the simulated pesticide (1.0, 12.0, and 24.0 mPa·s), and various spray outputs manipulated with PWM duty cycles. For each injection loop, the amounts of the chemical concentrate and water discharged into the mixing line were measured separately in response to preset target concentrations. The results showed that the measured concentrations were consistent across the patternator heights, spray viscosities, and duty cycle combinations. For all treatments, the mean absolute percentage error (MAPE) of the measured concentration was 6.96%, indicating that the concentration accuracy of the system was acceptable. The mean coefficient of variation was 3.35%, indicating that the spatial distribution uniformity of the system was in the desirable range. In addition, there was little variation in chemical concentration for spray mixtures collected at different heights on the patternator. Thus, the premixing in-line injection system could adequately dispense chemical concentrate and water to produce accurate concentrations and uniform spray mixtures for variable-rate nozzles to discharge to targets. Keywords: Environment protection, Precision pesticide application, Laser-guided sprayer, Tank mixture disposal, Specialty crop.","PeriodicalId":23120,"journal":{"name":"Transactions of the ASABE","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79093011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological Activity of the Extracts from Pecan Shelling Industry Byproducts","authors":"Canan Sevimli, Y. Gezgin, A. Oz, Shaymaa Al Sharqi, Z. P. Gumus, N. Dunford","doi":"10.13031/TRANS.14439","DOIUrl":"https://doi.org/10.13031/TRANS.14439","url":null,"abstract":"Highlights First study on characterization of the extracts obtained from industrial pecan nut processing byproducts. Chemical composition and biological activity of the extracts varied with the pecan cultivars used in the process. Pecan shell extracts exhibited anticancer, antioxidant and antimicrobial activity. Abstract Industrial processing of pecan nuts produces large amounts of shells. They have economic significance to pecan growers and processors. Hence, it is imperative that valorization of the pecan processing byproducts are explored. In this study, byproducts from commercial pecan nut shelling operations were evaluated as potential sources of biologically active phytochemicals. Shelling byproducts from Pawnee, Native and Stuart cultivars were examined. Aqueous ethanol shell extracts were analyzed for their chemical composition using the QTOF-MS technique. Total phenolic content, DPPH Radical Scavenging Capacity, anticancer and antimicrobial activity of the samples were investigated. Chemical composition and biological activity of the shell extracts varied significantly with the pecan cultivar and type of the byproduct stream used in the study. Presence of nut meat pieces in the byproducts resulted in high oil content in the extracts. The extract from Pawnee cultivar showed high DDPH activity, low IC50 for the cancer cell lines and high IC50 for the healthy cell line Vero. Extracts from all the cultivars had antimicrobial activity against Escherichia coli at relatively high disk loadings. This is the first report on the biological activity of extracts obtained from commercial pecan shelling operation byproducts. The findings of this study have significant practical implications and provide the initial data much needed for valorization of industrial byproducts.","PeriodicalId":23120,"journal":{"name":"Transactions of the ASABE","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78931147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dielectric Properties of Switchgrass and Corn Stover in the Radio Frequency Range","authors":"A. M. Souza, S. Birrell, B. Steward","doi":"10.13031/TRANS.13940","DOIUrl":"https://doi.org/10.13031/TRANS.13940","url":null,"abstract":"The dielectric properties of biological materials are relevant when developing moisture content sensors. However, little is known about the permittivities of switchgrass and corn stover in a wider frequency range. The goal of this research was to determine their dielectric constants and loss factors at different moisture contents across a frequency range of 5 Hz to 13 MHz and with the material static and in motion inside a sample container. The permittivity of these materials was calculated by measuring their admittance in a test fixture using an impedance analyzer at three different moisture levels (9.0% to 30.5%). Overall, the materials‘ dielectric properties increased with moisture but decreased with frequency. Prediction models were developed using the data in a frequency range of 10 kHz to 5 MHz. Model coefficients of determination were higher than 0.90 in general, except for the model measuring the loss factor of switchgrass in motion. Additionally, the dielectric constant was not different with the materials static or in motion, but the loss factor values were distinct. This work can be used for the development of electrical moisture content sensors for switchgrass and corn stover.","PeriodicalId":23120,"journal":{"name":"Transactions of the ASABE","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87910983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DRAINMOD-P: A Model for Simulating Phosphorus Dynamics and Transport in Drained Agricultural Lands: II. Model Testing","authors":"Manal H. Askar, M. Youssef, D. Hesterberg, K. King, A. Amoozegar, R. Skaggs, G. Chescheir, E. Ghane","doi":"10.13031/trans.14510","DOIUrl":"https://doi.org/10.13031/trans.14510","url":null,"abstract":"HighlightsDRAINMOD-P was tested using a dataset from a drained field with desiccation cracks.Surface and subsurface phosphorus losses were mainly in the particulate form.Surface runoff was a major pathway for phosphorus loss in this field.The model performance in predicting edge-of-field phosphorus loss is promising.Abstract. The recently developed phosphorus (P) model DRAINMOD-P was tested using a four-year dataset from a subsurface-drained field in northwest Ohio with significant potential for desiccation cracking or preferential flow. The model satisfactorily predicted subsurface drainage discharge, with a monthly Nash-Sutcliffe efficiency (NSE) of 0.59 and index of agreement (IOA) of 0.89. Lack of annual water budget closure was reported and was likely caused by uncertainty in measured surface runoff and/or modeling approaches representing macropore flow. More than 80% of predicted surface and subsurface P losses were in the particulate form. Surface runoff was the major pathway for P loss, contributing 78% of predicted total P (TP) load. On average, predicted macropore flow represented about 15% of drainage discharge and contributed 21% of DRP loss via subsurface drains. The performance of DRAINMOD-P in predicting monthly dissolved reactive P and TP losses through subsurface drains can be rated as poor (NSE = 0.33 and IOA = 0.60) and very good (NSE = 0.81 and IOA = 0.95), respectively. DRAINMOD-P demonstrated potential for simulating P fate and transport in drained cropland. More testing is needed to further examine newly incorporated hydrological and biogeochemical components of the model. Keywords: Agricultural drainage, Edge-of-field phosphorus load, Macropore flow, Phosphorus model, Sediment yield, Water quality modeling.","PeriodicalId":23120,"journal":{"name":"Transactions of the ASABE","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87919951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil Particle Modeling and Parameter Calibration for Use with Discrete Element Method","authors":"Yukun Yang, Baoqin Wen, Longpeng Ding, Liqiao Li, Xinghua Chen, Jingbin Li","doi":"10.13031/trans.14083","DOIUrl":"https://doi.org/10.13031/trans.14083","url":null,"abstract":"HighlightsSoil particle shapes were statistically analyzed, and four representative particles were obtained.A particle model was established using three-dimensional non-contact surface topography.This study used a response surface design method to calibrate significant soil parameters.The simulation parameters were verified by rotary tiller experiment.Abstract. The discrete element method (DEM) has broad prospects for application in soil-tool simulations. To ensure the reliability of simulations, appropriate simulation parameters and particle modeling are essential. Therefore, in this article, a method combining simulation and actual tests is proposed to calibrate the critical soil parameters. First, the effect of soil particle shape on particle contact was considered. Soil particle shapes were statistically analyzed using an improved GrabCut algorithm and k-means algorithm. Four representative soil particles were obtained. Second, a soil particle model was established by microscope and three-dimensional non-contact surface topography. Finally, taking the angle of repose as the response value, the three parameters with significant effects on the angle of repose, i.e., soil shear modulus, Hertz-Mindlin with Johnson-Kendall-Roberts contact model (JKR), and soil-soil restitution coefficient, were obtained via a Plackett-Burman experiment. The optimal value intervals of the significant parameters were determined by the steepest climbing test. A polynomial regression model between the angle of repose and the three significant parameters was established with a Box-Behnken experiment using three factors and three levels. The interactions between the three significant parameters were not significant, as revealed by response surface analysis. The optimal values of the significant parameters were obtained by taking the actual angle of repose as the target and resulted in a soil shear module of 9.8 MPa, JKR of 0.063, and soil-soil restitution coefficient of 0.478. To verify the reliability of the calibrated parameters, the soil angles of repose from the simulation and from actual tests were compared and analyzed. For a simulated angle of repose of 38.5°, the actual angle of repose was 38.6°, and the relative error was 0.26%. DEM was also used to simulate a rotary tiller with the calibrated parameters. The maximum error of the simulated soil throwing angle was less than 10% when compared with the actual throwing angle. The experimental results showed that the calibrated parameters were accurate and can provide a reference for the selection of soil discrete element parameters. Keywords: Angle of repose, Numerical simulation, Parameter calibration, Shape survey, Soil.","PeriodicalId":23120,"journal":{"name":"Transactions of the ASABE","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73459413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental Research on the Bending and Fracture Characteristics of Cotton Stalk","authors":"Peng Jiang, Yaping Li, Jiali Li, Hewei Meng, Xiangbin Peng, Bingcheng Zhang, Jiaxing He, Za Kan","doi":"10.13031/trans.14589","DOIUrl":"https://doi.org/10.13031/trans.14589","url":null,"abstract":"HighlightsA two-factor randomized block design was used to study the influence of experimental factors on indicators.Specific fracture energy can indicate the relationship between mass and power.A cotton stalk model was established using the discrete element method (DEM).Abstract. Effectively chopping of the mixture of mulch film and cotton stalk recycled by machine is the only way to achieve subsequent separation of the materials. Cotton stalk is one of the main components of the mixture. According to the working principle of a chopping device, the bending and fracture characteristics of cotton stalk samples were measured. A two-factor random block design was used to study the effects of moisture content and sample location on the plant on the mechanical characteristics of the stalk samples. According to the results, the specific fracture energy of the stalk samples was calculated. The results showed that the relationship between the moisture content and bending performance of the samples was an inverse proportional function in general. However, when the moisture content was 20% to 30%, the fracture energy in the double-support bending tests was low, which was therefore the most suitable condition for chopping. In addition, a cotton stalk model was established using the discrete element method (DEM), and the optimal parameter combination was determined. Compared with the actual test results, the model error of the peak bending force was 1.20%. This study can support the analysis of chopping device simulation and material preparation in experimental research. Keywords: Bending fracture characteristics, Cotton stalk, Discrete element method, Three-point bending test.","PeriodicalId":23120,"journal":{"name":"Transactions of the ASABE","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74373485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediction of Synchronization Time for Tractor Power-Shift Transmission Using Multi-Body Dynamic Simulation","authors":"Hyun-Woo Han, Jung-Su Han, W. Chung, Ji-Tae Kim, Young-Jun Park","doi":"10.13031/trans.14233","DOIUrl":"https://doi.org/10.13031/trans.14233","url":null,"abstract":"HighlightsPrediction of synchronization time was performed for a power-shift transmission.We derived an analytical equation for synchronization time and developed a multi-body dynamics model.Model results were compared with results of a power-shift test on a synchronizer.Reduced computation and design time was achieved for automatic transmission design.Abstract. Synchronization time determines the capacity of a shift actuator for an automatic transmission system. Existing approaches for measuring this time only consider one rotational inertia and therefore cannot be applied to the power-shift transmission (PST) of a tractor with wet multi-plate clutches on both sides of the synchronizer. This study aims to predict the PST synchronization time by considering time-varying axial forces as first-order functions and the equivalent rotational inertias of the hub and the gear. First, we derive an analytical equation for the synchronization time. We then develop a multi-body dynamics (MBD) model that includes the drag torque of the wet multi-plate clutches. The MBD model is composed of a synchronizer, a linkage, and an output shaft of a shift actuator as a rigid-body system. A power-shift test was performed on the synchronizer at two shift stages requiring the maximum shift force of the system. The torque of the shift actuator (the input of the shift system) and the angular displacement of the output shaft of the shift actuator (the output of the shift system) were measured. The results of the simulation model were then compared with those of the shift test. Compared with the test results, the simulation results were validated within 7.63% accuracy, based on the maximum value for the torque of the shift actuator. The proposed equation was validated within a maximum error range of 8.25%. The proposed equation did not consider drag torque of the wet multi-plate clutches because that torque is much smaller than the cone torque of the synchronizer in the target shift system. The proposed equation can reduce computation time and will enable more precise sizing of the synchronizer and shift actuator in the early design stages of automatic transmissions. Keywords: Multi-body dynamics, Power-shift transmission, Synchronization time, Synchronizer, Tractor transmission.","PeriodicalId":23120,"journal":{"name":"Transactions of the ASABE","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76636947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of Cover Crops for Water Pollutant Reduction from Agricultural Areas","authors":"R. Christianson, J. Fox, N. Law, Carol Wong","doi":"10.13031/trans.14028","DOIUrl":"https://doi.org/10.13031/trans.14028","url":null,"abstract":"HighlightsNitrogen loss reduction due to a cover crop tends to improve with increased cover crop biomass production.Mixed phosphorus loss reduction results in cold climates where freeze-thaw cycles occur and can increase dissolved phosphorus losses.Cereal rye was the primary cover crop studied and tended to provide the most water quality benefits.Abstract. Mitigating nutrient losses from agricultural fields retains these nutrients for subsequent crop production and reduces the risk to downstream water quality. This study evaluated the impact of cover crops, as part of an annual cropping system, on reducing nutrient losses and enhancing water quality. Cover crop literature focusing on water quality was reviewed to determine important factors regarding cover crop performance and cost. Results show that a grass-based cover crop and mixes with grasses tend to increase nitrate loss reduction (40%) compared to legumes (negligible). Biomass growth was also important, with early seeding or growth of a cover crop in areas with increased growing degree days enhancing performance. For phosphorus loss, benefits did not necessarily increase with increasing biomass. Further, dissolved phosphorus concentrations may increase due to freeze-thaw cycles (23%), although overall dissolved phosphorus losses tend to decrease due to less runoff (34%). Cover crop implementation costs ranged from a savings of $25 to $44 ha-1 year-1 before soybeans and corn, respectively, when implementing a cover crop for five straight years to a cost of $193 ha-1 year-1. Including a cover crop in annual crop rotations with adequate time in the fall for germination and growth can reduce nitrogen and phosphorus losses from production agriculture to help meet water quality goals across the U.S. Keywords: Catch crop, Nitrogen, NRCS, Phosphorus, Practice Code 340, USDA, Water quality.","PeriodicalId":23120,"journal":{"name":"Transactions of the ASABE","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77610826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperspectral Imaging with Cost-Sensitive Learning for High-Throughput Screening of Loblolly Pine (Pinus taeda L.) Seedlings for Freeze Tolerance","authors":"Yuzhen Lu, K. Payn, P. Pandey, J. Acosta, Austin J. Heine, Trevor D. Walker, Sierra N. Young","doi":"10.13031/TRANS.14708","DOIUrl":"https://doi.org/10.13031/TRANS.14708","url":null,"abstract":"HighlightsA hyperspectral imaging approach was developed for freeze-tolerance phenotyping of loblolly pine seedlings.Image acquisition was conducted before and periodically after artificial freezing of the seedlings.A hyperspectral data processing pipeline was developed to extract the spectra from seedling segments.Cost-sensitive support vector machine (SVM) was used for classifying stressed and healthy seedlings.Post-freeze scanning of seedlings on day 41 achieved the highest screening accuracy of 97%.Abstract. Loblolly pine (Pinus taeda L.) is a commercially important timber species planted across a wide temperature gradient in the southeastern U.S. It is critical to ensure that the planting stock is suitably adapted to the growing environment to achieve high productivity and survival. Long-term field studies, although considered the most reliable method for assessing cold hardiness of loblolly pine, are extremely resource-intensive and time-consuming. The development of a high-throughput screening tool to characterize and classify freeze tolerance among different genetic entries of seedlings will facilitate accurate deployment of highly productive and well-adapted families across the landscape. This study presents a novel approach using hyperspectral imaging to screen loblolly pine seedlings for freeze tolerance. A diverse population of 1549 seedlings raised in a nursery were subjected to an artificial mid-winter freeze using a freeze chamber. A custom-assembled hyperspectral imaging system was used for in-situ scanning of the seedlings before and periodically after the freeze event, followed by visual scoring of the frozen seedlings. A hyperspectral data processing pipeline was developed to segment individual seedlings and extract the spectral data. Examination of the spectral features of the seedlings revealed reductions in chlorophylls and water concentrations in the freeze-susceptible plants. Because the majority of seedlings were freeze-stressed, leading to severe class imbalance in the hyperspectral data, a cost-sensitive learning technique that aims to optimize a class-specific cost matrix in classification schemes was proposed for modeling the imbalanced hyperspectral data, classifying the seedlings into healthy and freeze-stressed phenotypes. Cost optimization was effective for boosting the classification accuracy compared to regular modeling that assigns equal costs to individual classes. Full-spectrum, cost-optimized support vector machine (SVM) models achieved geometric classification accuracies of 75% to 78% before and within 10 days after the freeze event, and up to 96% for seedlings 41 days after the freeze event. The top portions of seedlings were more indicative of freeze events than the middle and bottom portions, leading to better classification accuracies. Further, variable selection enabled significant reductions in wavelengths while achieving even better accuracies of up to 97% than full-spectrum SVM modeling. This study ","PeriodicalId":23120,"journal":{"name":"Transactions of the ASABE","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78723058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}