Tissue engineering最新文献_第2页

Design of silk-like biomaterials inspired by mussel-adhesive protein. 丝质生物材料的设计灵感来自于贻贝黏附蛋白。

Tissue engineering Pub Date : 2007-12-01 DOI: 10.1089/ten.2006.0448

Mingying Yang, Kazuo Yamauchi, Masato Kurokawa, Tetsuo Asakura

引用次数: 26

Strategic directions in tissue engineering. 组织工程的战略方向。

Tissue engineering Pub Date : 2007-12-01 DOI: 10.1089/ten.2007.0335

Peter C Johnson, Antonios G Mikos, John P Fisher, John A Jansen

引用次数: 141

Microvascular transplantation after acute myocardial infarction. 急性心肌梗死后微血管移植。

Tissue engineering Pub Date : 2007-12-01 DOI: 10.1089/ten.2007.0025

Benjamin R Shepherd, James B Hoying, Stuart K Williams

{"title":"Microvascular transplantation after acute myocardial infarction.","authors":"Benjamin R Shepherd, James B Hoying, Stuart K Williams","doi":"10.1089/ten.2007.0025","DOIUrl":"https://doi.org/10.1089/ten.2007.0025","url":null,"abstract":"The primary objective of this study was to evaluate epicardial transplantation of an intact microvascular network for treatment of myocardial ischemia in a murine model of acute myocardial infarction. We describe transplantation of an intact microvascular network constructed from isolated microvascular segments stabilized in a 3-dimensional matrix to the epicardial surface after acute myocardial infarction. This microvascular graft was implanted as a patch on the epicardium of mice after left coronary artery ligation. After 14 and 28 days of implantation, left ventricular (LV) function was assessed and grafts evaluated via histology and cytochemistry. Inosculation of microvessels within the graft with host coronary microcirculation occurred as early as 7 days after initial tissue grafting. Morphologic evaluation of the grafts revealed arterioles, venules, capillaries, and erythrocytes within vascular lumina. Control grafts of collagen alone remained avascular. LV infarct size was smaller, and LV function improved in treated animals. Engraftment of whole microvascular units can be achieved to support cell-assisted vascular remodeling. Microvascular grafts may provide therapeutic benefit as a primary treatment or serve as a microvascular platform for cardiac repair and regeneration.","PeriodicalId":23102,"journal":{"name":"Tissue engineering","volume":" ","pages":"2871-9"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/ten.2007.0025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40986517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 68

Fabrication of an optimal urethral graft using collagen-sponge tubes reinforced with Copoly(L-lactide/epsilon-caprolactone) fabric. 用胶原-海绵管和共聚(l -丙交酯/epsilon-己内酯)织物增强的尿道移植物的制备。

Tissue engineering Pub Date : 2007-12-01 DOI: 10.1089/ten.2007.0052

Isao Kanatani, Akihiro Kanematsu, Yasuyuki Inatsugu, Masaaki Imamura, Hiromitsu Negoro, Noriyuki Ito, Shingo Yamamoto, Yasuhiko Tabata, Yoshito Ikada, Osamu Ogawa

{"title":"Fabrication of an optimal urethral graft using collagen-sponge tubes reinforced with Copoly(L-lactide/epsilon-caprolactone) fabric.","authors":"Isao Kanatani, Akihiro Kanematsu, Yasuyuki Inatsugu, Masaaki Imamura, Hiromitsu Negoro, Noriyuki Ito, Shingo Yamamoto, Yasuhiko Tabata, Yoshito Ikada, Osamu Ogawa","doi":"10.1089/ten.2007.0052","DOIUrl":"https://doi.org/10.1089/ten.2007.0052","url":null,"abstract":"An ideal biomaterial for urethral reconstruction has not been developed. To create a urethral graft biomaterial with optimal biodegradability and biocompatibility, a copoly(L-lactide/epsilon-caprolactone) [P(LA/CL)] fabric tube was combined with a type I collagen sponge. The P(LA/CL) fibers were knitted into a vascular stent style (Type 1) or weaved into a mesh style (Type 2) to prepare P(LA/CL) tubes. The tubes were dipped in aqueous collagen solution and lyophilyzed to prepare the P(LA/CL)-collagen sponge graft. The grafts were applied to a 1.5-cm rabbit urethral defect (n = 14 for each condition), and tissue repair was evaluated using urethrographical, urethroscopical, and histological examination 1, 3, and 6 months after surgery. Although epithelialization was observed after 1 month in all Type 1 grafts, stenoses, fistulae, or stone formation was seen in 7 of the rabbits. In some cases, P(LA/CL) fibers prolapsed into the urethral lumen, causing stone formation. Only 3 rabbits survived for 6 months, and 2 of these had stenoses. For the Type 2 graft, all urethras were patent, without fistulae or stenoses, over the entire observation period. Histologically, urethral structure was disorganized for the Type 1 graft, whereas the urethral tissue on the Type 2 graft was slightly fibrotic but completely epithelialized and supported by a regenerated smooth muscle layer at 6 months. These findings suggest that creation of a scaffold suitable for urethral tissue regeneration will depend not only on the biomaterial composition, but also on the fabrication technique.","PeriodicalId":23102,"journal":{"name":"Tissue engineering","volume":"13 12","pages":"2933-40"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/ten.2007.0052","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27069059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Integrated 3-dimensional expansion and osteogenic differentiation of murine embryonic stem cells. 小鼠胚胎干细胞的整合三维扩增与成骨分化。

Tissue engineering Pub Date : 2007-12-01 DOI: 10.1089/ten.2007.0072

Wesley L Randle, Jae Min Cha, Yu-Shik Hwang, K L Andrew Chan, Sergei G Kazarian, Julia M Polak, Athanasios Mantalaris

{"title":"Integrated 3-dimensional expansion and osteogenic differentiation of murine embryonic stem cells.","authors":"Wesley L Randle, Jae Min Cha, Yu-Shik Hwang, K L Andrew Chan, Sergei G Kazarian, Julia M Polak, Athanasios Mantalaris","doi":"10.1089/ten.2007.0072","DOIUrl":"https://doi.org/10.1089/ten.2007.0072","url":null,"abstract":"Embryonic stem cell (ESC) culture is fragmented and laborious and involves operator decisions. Most protocols consist of 3 individual steps: maintenance, embryoid body (EB) formation, and differentiation. Integration will assist automation, ultimately aiding scale-up to clinically relevant numbers. These problems were addressed by encapsulating undifferentiated murine ESCs (mESCs) in 1.1% (w/v) low-viscosity alginic acid, 0.1% (v/v) porcine gelatin hydrogel beads (d = 2.3 mm). Six hundred beads containing 10,000 mESCs per bead were cultured in a 50-mL high-aspect-ratio vessel bioreactor. Bioreactor cultures were rotated at 17.5 revolutions per min, cultured in maintenance medium containing leukemia inhibitory factor for 3 days, replaced with EB formation medium for 5 days followed by osteogenic medium containing L-ascorbate-2-phosphate (50 microg/mL), beta-glycerophosphate (10 mM), and dexamethasone (1 microM) for an additional 21 days. After 29 days, 84 times as many cells per bead were observed and mineralized matrix was formed within the alginate beads. Osteogenesis was confirmed using von Kossa, Alizarin Red S staining, alkaline phosphatase activity, immunocytochemistry for osteocalcin, OB-cadherin, collagen type I, reverse transcriptase polymerase chain reaction, microcomputed tomography (micro-computed tomography) and Fourier transform infrared spectroscopic imaging. This simplified, integrated, and potentially scaleable methodology could enable the production of 3-demensional mineralized tissue from ESCs for potential clinical applications.","PeriodicalId":23102,"journal":{"name":"Tissue engineering","volume":" ","pages":"2957-70"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/ten.2007.0072","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41019148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 75

In vitro assessment of axonal growth using dorsal root ganglia explants in a novel three-dimensional collagen matrix. 在新型三维胶原基质中使用背根神经节外植体对轴突生长的体外评估。

Tissue engineering Pub Date : 2007-12-01 DOI: 10.1089/ten.2007.0116

Ahmet Bozkurt, Gary A Brook, Sven Moellers, Franz Lassner, Bernd Sellhaus, Joachim Weis, Michael Woeltje, Julian Tank, Christina Beckmann, Paul Fuchs, Leon Olde Damink, Frank Schügner, Ingo Heschel, Norbert Pallua

{"title":"In vitro assessment of axonal growth using dorsal root ganglia explants in a novel three-dimensional collagen matrix.","authors":"Ahmet Bozkurt, Gary A Brook, Sven Moellers, Franz Lassner, Bernd Sellhaus, Joachim Weis, Michael Woeltje, Julian Tank, Christina Beckmann, Paul Fuchs, Leon Olde Damink, Frank Schügner, Ingo Heschel, Norbert Pallua","doi":"10.1089/ten.2007.0116","DOIUrl":"https://doi.org/10.1089/ten.2007.0116","url":null,"abstract":"The goal of this study was the development of a bioartificial nerve guide to induce axonal regeneration in the peripheral nervous system (PNS). In this in vitro study, the ability of a novel, 3-dimensional (3D), highly oriented, cross-linked porcine collagen scaffold to promote directed axonal growth has been studied. Collagen nerve guides with longitudinal guidance channels were manufactured using a series of chemical and mechanical treatments with a patented unidirectional freezing process, followed by freeze-drying (pore sizes 20-50 microm). Hemisected rat dorsal root ganglia (DRG) were positioned such that neural and non-neural elements could migrate into the collagen scaffold. After 21 days, S100-positive Schwann cells (SCs) migrated into the scaffold and aligned within the guidance channels in a columnar fashion, resembling \"Bands of Büngner.\" Neurofilament-positive axons (mean length +/- SD 756 microm +/- 318 microm, maximum 1496 microm) from DRG neurons entered the scaffold where the growth within the guidance channels was closely associated with the oriented SCs. This study confirmed the importance of SCs in the regeneration process (neurotrophic theory). The alignment of SCs within the guidance channels supported directional axonal growth (contact guidance theory). The microstructural properties of the scaffold (open, porous, longitudinal pore channels) and the in vitro data after DRG loading (axonal regeneration along migrated and columnar-aligned SCs resembling \"Band of Büngner\") suggest that this novel oriented 3D collagen scaffold serves as a basis for future experimental regeneration studies in the PNS.","PeriodicalId":23102,"journal":{"name":"Tissue engineering","volume":"13 12","pages":"2971-9"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/ten.2007.0116","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27048629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 165

Dependence of neovascularization mechanisms on the molecular microenvironment. 新生血管形成机制对分子微环境的依赖性。

Tissue engineering Pub Date : 2007-12-01 DOI: 10.1089/ten.2007.0031

Eliane R Popa, Barry W A van der Strate, Linda A Brouwer, Henko Tadema, Martin Schipper, Brian Fernandes, Marc Hendriks, Marja J A van Luyn, Martin C Harmsen

{"title":"Dependence of neovascularization mechanisms on the molecular microenvironment.","authors":"Eliane R Popa, Barry W A van der Strate, Linda A Brouwer, Henko Tadema, Martin Schipper, Brian Fernandes, Marc Hendriks, Marja J A van Luyn, Martin C Harmsen","doi":"10.1089/ten.2007.0031","DOIUrl":"https://doi.org/10.1089/ten.2007.0031","url":null,"abstract":"In vivo vascularization of implanted (bio)artificial constructs is essential for their proper function. Vascularization may rely on sprouting angiogenesis, vascular incorporation of bone marrow-derived endothelial cells (BMDECs), or both. Here we investigated the relative contribution of these 2 mechanisms to neovascularization in a mouse model of a foreign body reaction (FBR) to subcutaneously implanted Dacron and in hind limb ischemia (HLI) in relation to the molecular microenvironment at these neovascularization sites. Neovascularization was studied in C57Bl/6 mice reconstituted with enhanced green fluorescent protein (EGFP) transgenic bone marrow. Sprouting angiogenesis, detected using nuclear incorporation of bromodeoxyuridine in endothelial cells was present in both models, whereas vascular incorporation of EGFP(+) BMDECs was restricted to HLI. In HLI, the presence of a pro-angiogenic molecular microenvironment comprising vascular endothelial growth factor, fibroblast growth factor 2, and granulocyte colony-stimulating factor corroborated the importance of these factors for vascular BMDEC incorporation, whereas this microenvironment was absent in FBR. Enhanced mobilization of BMDECs by granulocyte-macrophage colony-stimulating factor administration or by combining HLI and FBR with Dacron did not induce incorporation of BMDECs in FBR neovessels. We conclude that the efficacy of BMDEC-based therapy is not generally warranted, but it depends on the molecular microenvironment in the targeted tissue.","PeriodicalId":23102,"journal":{"name":"Tissue engineering","volume":" ","pages":"2913-21"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/ten.2007.0031","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41036175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Vascularized adipose tissue grafts from human mesenchymal stem cells with bioactive cues and microchannel conduits. 利用生物活性线索和微通道导管从人类间充质干细胞中提取血管化脂肪组织移植物。

Tissue engineering Pub Date : 2007-12-01 DOI: 10.1089/ten.2007.0078

Michael S Stosich, Barb Bastian, Nicholas W Marion, Paul A Clark, Gwendolen Reilly, Jeremy J Mao

{"title":"Vascularized adipose tissue grafts from human mesenchymal stem cells with bioactive cues and microchannel conduits.","authors":"Michael S Stosich, Barb Bastian, Nicholas W Marion, Paul A Clark, Gwendolen Reilly, Jeremy J Mao","doi":"10.1089/ten.2007.0078","DOIUrl":"10.1089/ten.2007.0078","url":null,"abstract":"Vascularization is critical to the survival of engineered tissues. This study combined biophysical and bioactive approaches to induce neovascularization in vivo. Further, we tested the effects of engineered vascularization on adipose tissue grafts. Hydrogel cylinders were fabricated from poly(ethylene glycol) diacrylate (PEG) in four configurations: PEG alone, PEG with basic fibroblast growth factor (bFGF), microchanneled PEG, or both bFGF-adsorbed and microchanneled PEG. In vivo implantation revealed no neovascularization in PEG, but substantial angiogenesis in bFGF-adsorbed and/or microchanneled PEG. The infiltrating host tissue consisted of erythrocyte-filled blood vessels lined by endothelial cells, and immunolocalized to vascular endothelial growth factor (VEGF). Human mesenchymal stem cells were differentiated into adipogenic cells, and encapsulated in PEG with both microchanneled and adsorbed bFGF. Upon in vivo implantation subcutaneously in immunodeficient mice, oil red O positive adipose tissue was present and interspersed with interstitial fibrous (IF) capsules. VEGF was immunolocalized in the IF capsules surrounding the engineered adipose tissue. These findings suggest that bioactive cues and/or microchannels promote the genesis of vascularized tissue phenotypes such as the tested adipose tissue grafts. Especially, engineered microchannels may provide a generic approach for modifying existing biomaterials by providing conduits for vascularization and/or diffusion.","PeriodicalId":23102,"journal":{"name":"Tissue engineering","volume":"13 12","pages":"2881-90"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4035031/pdf/nihms-232010.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27008823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ECM molecules mediate both Schwann cell proliferation and activation to enhance neurite outgrowth. ECM分子介导雪旺细胞增殖和激活，促进神经突生长。

Tissue engineering Pub Date : 2007-12-01 DOI: 10.1089/ten.2007.0055

Stephanie J Armstrong, Mikael Wiberg, Giorgio Terenghi, Paul J Kingham

{"title":"ECM molecules mediate both Schwann cell proliferation and activation to enhance neurite outgrowth.","authors":"Stephanie J Armstrong, Mikael Wiberg, Giorgio Terenghi, Paul J Kingham","doi":"10.1089/ten.2007.0055","DOIUrl":"https://doi.org/10.1089/ten.2007.0055","url":null,"abstract":"Tissue engineering using a combination of biomaterials and cells represents a new approach to nerve repair. We have investigated the effect that extracellular matrix (ECM) molecules have on Schwann cell (SC) attachment and proliferation on the nerve conduit material poly-3-hydroxybutyrate (PHB), and SC influence on neurite outgrowth in vitro. Initial SC attachment to PHB mats was unaffected by ECM molecules but proliferation increased (laminin > fibronectin > collagen). SCs seeded onto ECM-coated culture inserts suspended above a monolayer of NG108-15 cells determined the effect of released diffusible factors. The effect of direct contact between the two cell types on ECM molecules was also investigated. In both systems SCs enhanced neurite number per cell and percentage of NG108-15 cells sprouting neurites. NG108-15 cells grown in direct contact with SCs had significantly longer neurites than those exposed to diffusible factors when seeded on laminin or fibronectin. Diffusible factors released from SCs cultured on ECM molecules appear to initiate neurite outgrowth, whereas SC-neuron contact promotes neurite elongation. SC proliferation was maximal on poly-D-lysine-coated surfaces, but these cells did not influence neurite outgrowth to the levels of laminin or fibronectin. This suggests that ECM molecules enhance cell number and activate SCs to release neurite promoting factors. Addition of ECM molecules to PHB nerve conduits containing SCs is likely to provide benefits for the treatment of nerve injuries.","PeriodicalId":23102,"journal":{"name":"Tissue engineering","volume":"13 12","pages":"2863-70"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/ten.2007.0055","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26912726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 140

Neonatal pig liver-derived progenitors for insulin-producing cells: an in vitro study. 新生猪肝脏来源的胰岛素生成细胞祖细胞:一项体外研究。

Tissue engineering Pub Date : 2007-12-01 DOI: 10.1089/ten.2007.0098

Leda Racanicchi, Giuseppe Basta, Pia Montanucci, Lucia Guido, Alessandra Pensato, Valentina Conti, Riccardo Calafiore

{"title":"Neonatal pig liver-derived progenitors for insulin-producing cells: an in vitro study.","authors":"Leda Racanicchi, Giuseppe Basta, Pia Montanucci, Lucia Guido, Alessandra Pensato, Valentina Conti, Riccardo Calafiore","doi":"10.1089/ten.2007.0098","DOIUrl":"https://doi.org/10.1089/ten.2007.0098","url":null,"abstract":"Beta (beta)-cell replacement represents an attractive approach for the possible cure of type 1 insulin-dependent diabetes mellitus (IDDM). In a search for potential sources of insulin-secreting cells for IDDM substitution therapy, we have focused on the neonatal pig liver, which is putatively enriched in multipotent stem cells. We then isolated cells measuring 10 to 15 microm in diameter, identified as small cells, characterized by a high proliferation rate and positive staining for immature liver and pancreatic endocrine cell markers (i.e., insulin and pancreatic duodenal homeobox). The ability of these cells to transdifferentiate into pancreatic beta-like cells under culture conditions with exendin-4 (Ex-4) or high glucose concentration was examined. We observed that insulin secretion was not physiological in basal conditions, although it became responsive to glucose after 5 days of exposure to Ex-4. This beta-cell-like phenotype remained physiologically stable, even after stimulus withdrawal. Based on these observations, we contend that the proposed cell and tissue model might offer several advantages as a candidate for substitution cell therapy in IDDM, because the neonatal pig liver seems enriched in cells, with a mixed pancreas-liver phenotype, that are easier to purify and grow in culture and are more functional than other beta-like cells upon in vitro single short-term stimulation challenge.","PeriodicalId":23102,"journal":{"name":"Tissue engineering","volume":"13 12","pages":"2923-31"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/ten.2007.0098","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27031267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7