Rapid Communications in Mass Spectrometry最新文献

{"title":"A New Approach to the Analysis of Dissolved Gases Using Passive Samplers, With Portable Mass Spectrometry and Gas Chromatography Techniques","authors":"Antoine Picard,&nbsp;Florent Barbecot,&nbsp;Matéo Lacheux,&nbsp;Matthias S. Brennwald,&nbsp;José A. Corcho-Alvarado","doi":"10.1002/rcm.70118","DOIUrl":"10.1002/rcm.70118","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>Portable mass spectrometry systems such as miniRUEDI enable rapid and low-cost field analysis of dissolved gases with high temporal resolution. However, they remain poorly suited for discrete small-volume water samples. This study presents an approach based on passive sampling for groundwater and surface waters dissolved gases and gas tracer analyses.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Passive samplers (≈25 mL) were deployed in near-air-saturated water for several days to equilibrate with dissolved gases. After retrieval and locking, gas samples were analysed in the laboratory using the miniRUEDI portable mass spectrometry combined with gas chromatography–electron capture detection (GC-ECD).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The combined miniRUEDI and GC-ECD approach enabled reliable and simultaneous measurements of major gases (N₂ and O₂), noble gases (He, Ar and Kr), and transient tracers including SF₆, CFC-12 and CFC-113 from a single 25 mL gas sample. The time required for complete equilibration within the passive samplers was found to be approximately 2 days.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This method extends portable mass spectrometry to discrete low-volume water samples and facilitates the use of transient anthropogenic gas tracers for determining young groundwater ages (&lt; 70 years). Its simplicity, low cost and multi-gas capability also make it promising for tracer plume mapping and environmental monitoring applications.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"40 17","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/rcm.70118","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148248150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toward Metabolomics Analyses With Combined Capillary Vibrating Sharp-Edge Spray Ionization and Atmospheric Pressure Chemical Ionization","authors":"Madison Pursell,&nbsp;Adefolake Ojanuga,&nbsp;Chandrima Banerjee,&nbsp;Ephraim Ezeigbo,&nbsp;Mohammad Rahman,&nbsp;Vikum K. Dewasurendra,&nbsp;Thomas B. Hughart,&nbsp;Matthew B. Johnson,&nbsp;Mst Nigar Sultana,&nbsp;Xiaoting Guo,&nbsp;Gabriel Alexander,&nbsp;Priya Patel,&nbsp;Peng Li,&nbsp;Justin Legleiter,&nbsp;Stephen J. Valentine","doi":"10.1002/rcm.70110","DOIUrl":"10.1002/rcm.70110","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>The varied properties of metabolomics components dictate the tools used in mass spectrometry (MS) characterization including the selection of the ionization source. However, the ionization of select compounds limits characterization efficiency. A simple setup employing vibrating sharp-edge spray ionization (VSSI) accesses two distinct ionization processes that can be performed cyclically during LC.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The MS approach described here couples analyte sample nebulization by VSSI with corona discharge to achieve ionization via an atmospheric pressure chemical ionization (APCI)-like process. A 28-component small molecule standard mixture as well as a <i>C. elegans</i> metabolite extract are analyzed to determine potential for utilization in metabolomics experiments.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>For the standard panel, the cVSSI-APCI approach is shown to better ionize half of all compounds compared to ESI. For the metabolomics extract, cVSSI-APCI is shown to significantly outperform ESI for multiple compounds across a range of reversed-phase LC solution conditions, while ESI is shown to outperform cVSSI-APCI for other species. Manual cycling between field-enabled cVSSI and cVSSI-APCI ionization processes results in relatively rapid equilibration to distinct ionization processes with high reproducibility in compound ionization.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Cycling between ionization processes is accomplished on a timescale suitable with longer timescale LC runs. To obtain sufficient cycling rates for high-throughput metabolomics experiments (~10 to 20 min) will require the use of high-voltage, high-power pulser electronics. The preliminary results suggest such an approach will be successful for online analyses.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"40 17","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/rcm.70110","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148248399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating Aptasensor With Mass-Tag Signal Amplification Strategy for the Detection of Three Steroid Hormones in Serum","authors":"Jia Zhao,&nbsp;Huimin Liu,&nbsp;Liujuan Zhan,&nbsp;Wei Shao,&nbsp;Guangming Huang","doi":"10.1002/rcm.70121","DOIUrl":"10.1002/rcm.70121","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>Steroid hormone testing is critical for assessing endocrine function, diagnosing related disorders, and monitoring therapeutic efficacy. However, current mainstream detection methods have limitations. Although liquid chromatography–tandem mass spectrometry (LC–MS/MS), regarded as the gold standard, offers high sensitivity and specificity, it involves complex and time-consuming procedures. Immunoassays such as enzyme-linked immunosorbent assay (ELISA) are simple and fast but are limited by poor throughput for multitarget detection. Therefore, it is crucial to develop an analytical method that streamlines procedures and enables efficient parallel detection of multiple targets.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We developed an integrated signal-enhanced aptasensor platform for pooled MS detection of three steroid hormones in serum. It combines aptamer recognition with mass-tag amplification. This approach uses biorecognition instead of chromatography, while mass spectrometry enables simultaneous readout of multiple mass tags from combined samples.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The method was evaluated by detecting three steroid hormones (vitamin D, cortisol, and testosterone) in simulated serum samples and human serum samples. The limits of detection (LODs) for these analytes ranged from 0.411 to 6.796 nM, which are below the established clinical cut-off values for each steroid, demonstrating the requisite sensitivity for detection.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This integrated signal-enhanced aptasensor outperforms conventional LC–MS/MS in efficiency and ELISA in throughput, enabling the quantification of multiple serum steroid hormones. Therefore, we believe that this method could be potentially useful in the clinical screening of hormone-related disorders and suitable for the analysis of serum.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"40 17","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148248196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipidome and Targeted Metabolome Analysis of Locational Picrorhiza Kurroa Royle. Ex Benth., Using UPLC-PDA and UHPLC-QTOF-IMS","authors":"Bindu Rawat,&nbsp;Robin Joshi,&nbsp;Dinesh Kumar","doi":"10.1002/rcm.70108","DOIUrl":"10.1002/rcm.70108","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Lipids are a fundamental class of biomolecules essential for membrane structure, energy storage, and cellular signaling, whereas lipidomics is an advanced dedicated way to understand the lipids, including biological lipids.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Keeping in mind the importance of lipid makeup, the current study was focused to understand the lipid diversity present within <i>Picrorhiza kurroa</i> (leaves, rhizomes, and roots) at three different localities of Himachal Pradesh, India: Bhatori (Pangi), Bharmaur (Chamba), and Gumna (Shimla).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Method</h3>\u0000 \u0000 <p>UHPLC-QTOF-IMS was used for lipid profiling of <i>P. kurroa</i> locational samples. The data were analyzed for each extraction parameter. Moreover, targeted specialized metabolites were also analyzed using UPLC-PDA. Further, multivariate analysis including supervised orthogonal projections to latent structures discriminant analysis and unsupervised principal component analysis were employed to understand the lipid makeup, its dissemination, and similarity traits among the samples.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Q-TOF/MS revealed the comprehensive lipidome of <i>P. kurroa</i> that includes the important presence of Cer, FA, (L) PE, TG, PA, PI, PS, MGDG, and DGDG. Leaves of <i>P. kurroa</i> collected from the Pangi region showed higher lipid content than other targeted parts and localities, whereas targeted metabolites including picrosides were present higher in the rhizomes of the Pangi region. Further, statistical analysis showed clear dissemination and similarity traits among the samples. The current study suggested variability among the chemo-profile of different locational samples.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>We concluded that leaves of <i>P. kurroa</i> collected from the Pangi region constitute higher lipid content than other parts and <i>P. kurroa</i> plants collected from another area. Also, the findings revealed that the plant <i>P. kurroa</i> contains Cer, FA, (L)PE, TG, DG, PG, PA, PI, PS, MGDG, and DGDG. The variation in picroside contents was also observed through UPLC-DAD analysis; rhizomes collected from the Bhatori (Pangi) region exhibited the highest picroside content.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"40 17","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148248199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic Profiling of Plasma and Urine of Benzo(a)pyrene-Induced Mouse Models of Lung Cancer","authors":"Nitin Bhardwaj,&nbsp;Ashutosh Singh,&nbsp; Rambabu","doi":"10.1002/rcm.70119","DOIUrl":"10.1002/rcm.70119","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Lung cancer (LC) is one of the most common malignancies with a high fatality rate. The current study recognizes the important metabolites and metabolic pathways in the plasma and urine of a BaP-induced mice model of LC.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods and Results</h3>\u0000 \u0000 <p>LC was generated by administering BaP (50-mg/kg body weight, twice a week for 1 month) orally. Plasma and urine samples were collected after 4 months of BaP treatment and derivatized with BSTFA and 1% TMCS. The metabolomics analysis was performed by using the gas chromatography mass spectrometry (GC–MS), and metabolites were recognized by annotating the peaks with the NIST mass spectra. There were 19 compounds in plasma and 48 compounds in urine, belonging to amino acids, fatty acids, sugars, and some organic categories, showing significant metabolic perturbations. The PCA and PLS-DA models also suggest a perfect discrimination between the metabolites in the plasma and urine of control and tumor-bearing mice. The VIP plot identifies nine compounds in plasma and in urine with maximum VIP scores. The maximum AUC value compounds in plasma (trimethyl phosphate, L-isoleucine, L-proline, 3,3′-oxydipropanol, L-glutamic acid, and 9-octadecenoic acid) and in urine (glycine, L-aspartic acid, L-5-oxoproline, butanedioic acid, glyceric acid, and butanoic acid) could be used as potential diagnostic markers in LC.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The metabolomics profiling of plasma and urine suggests a significant alteration in the relative abundance of fatty acids, sugars, and amino acids as well as potential biomarkers for the detection of LC.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"40 17","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148248331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"40 16","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148094668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"40 16","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148091889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"40 16","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148094372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"40 16","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148094373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"40 16","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148087426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}