Rapid Communications in Mass Spectrometry最新文献

{"title":"An Improved Chemical Extraction Procedure for the Sr Isotope Analysis of Liquid Agrifood Samples Applied to Authenticating the Origin of Maple Syrups in Quebec (Canada)","authors":"B. Saar de Almeida,&nbsp;R. Stevenson,&nbsp;M. Sadiki,&nbsp;L. Lagacé,&nbsp;D. Widory","doi":"10.1002/rcm.10066","DOIUrl":"10.1002/rcm.10066","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>We describe a new simplified sample preparation technique, based on dual chromatography for the analysis of ⁸⁷Sr/⁸⁶Sr in liquid agri-food samples. We applied this approach for authenticating the origin of maple syrup products in Quebec, characterizing the ⁸⁷Sr/⁸⁶Sr of soil profiles from geologically distinct maple groves, including maple tree components and maple syrup products.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In our simplified technique, 3 mL of the organic liquids was poured into a 15-mL centrifuge tube, diluted with 10 mL of Mill-Q H₂O, and manually shaken to produce a thin juice. The thin juice was subsequently loaded in two 7.5 mL aliquots onto 10-mL Bio-RadTM chromatography columns containing 4 mL of cleaned AG50-X8 resin (100–200 mesh in a 1-N HCl solution). Once the sample was absorbed by the resin, the organic fraction of the thin juice was eluted by adding 3 × 5 mL of 1-N HCl. The Sr and other cations were subsequently recovered by adding 2 × 5 mL of 6-N HCl.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The maple groves and the ⁸⁷Sr/⁸⁶Sr ratios for maple syrup from 39 different producing areas in Quebec indicated that no isotope fractionation occurs between the syrup, the maple trees, and the corresponding labile fraction of the soil they grew upon. This suggests that ⁸⁷Sr/⁸⁶Sr provides a reliable isotope fingerprint for the provenance of maple syrups. Gathering available agri-food ⁸⁷Sr/⁸⁶Sr data across the Quebec province, we constructed the first bioavailable ⁸⁷Sr/⁸⁶Sr map based on actual agri-food data.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This study reported an improved method for Sr separation for ⁸⁷Sr/⁸⁶Sr studies in liquids by combining two different cation-exchange chromatography steps. Data were used to develop a bioavailable ⁸⁷Sr/⁸⁶Sr map that can be used to predict the geographical origin of agrifood products from southern Quebec.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"39 16","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/rcm.10066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143944681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LC-IRMS Persulfate Oxidation: Case Study on Neonicotinoid-Related Structures","authors":"Felix Niemann,&nbsp;Annika Gruhlke,&nbsp;Maik A. Jochmann,&nbsp;Torsten C. Schmidt","doi":"10.1002/rcm.10067","DOIUrl":"10.1002/rcm.10067","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>Liquid chromatography-isotope ratio mass spectrometry (LC-IRMS) is used to analyze stable carbon isotope ratios of polar nonvolatile compounds. However, challenges with the persulfate-based oxidation interface have been reported, particularly for molecules with recalcitrant structures like those found in neonicotinoids. This study systematically investigates the oxidation efficiency of neonicotinoid-related structures in a commercial LC-IRMS.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Neonicotinoid proxies of varying molecular complexity were evaluated for carbon recovery and stable carbon isotope ratio accuracy. LC-IRMS parameters such as oxidant concentration, reaction time, temperature, acid concentration, and the presence of AgNO₃ catalyst were varied. Carbon recoveries and <i>δ</i>¹³C biases were determined by injecting an oxidation-independent inorganic carbon standard under identical conditions. Elemental analyzer isotope ratio mass spectrometry (EA-IRMS) was used to normalize <i>δ</i>¹³C values.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Several neonicotinoid derivatives exhibited low carbon recovery and significant <i>δ</i>¹³C bias. Increasing oxidant concentration, reactor temperature, and reaction time improved recoveries but did not fully mitigate isotopic biases. The addition of AgNO₃ improved carbon recoveries for most derivatives but introduced variability in <i>δ</i>¹³C values, likely due to shifts in reaction mechanisms. A workflow to identify oxidation problems during method development was proposed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Optimization of LC-IRMS oxidation parameters is critical for urea, guanidine, and nitroguanidine derivatives and similar compounds. A systematic evaluation of oxidation efficiencies under different conditions is needed for optimal mineralization and thus more accurate <i>δ</i>¹³C ratios.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"39 16","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/rcm.10067","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143938831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dried Blood Spot Analysis for Simultaneous Quantification of Antiepileptic Drugs Using Liquid Chromatography–Tandem Mass Spectrometry","authors":"Mariam M. Abady,&nbsp;Ji-Seon Jeong,&nbsp;Ha-Jeong Kwon","doi":"10.1002/rcm.10064","DOIUrl":"10.1002/rcm.10064","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>Dried blood spot (DBS) sampling for the therapeutic drug monitoring of antiepileptic drugs offers practical advantages, including minimal invasiveness and ease of collection. However, for precise therapeutic management, its accuracy and reliability in quantification need to be validated.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This study validates DBS sampling for the analysis of 11 antiepileptic drugs using liquid chromatography-tandem mass spectrometry (LC-MS/MS), overcoming the physicochemical challenges associated with DBS samples.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The acetonitrile-based DBS extraction method demonstrated high efficiency for the 11 antiepileptic drugs. Accuracy and precision within 6% were achieved in both intra- and inter-day assays, with good selectivity, minimal matrix effects, and negligible carryover. All antiepileptic drugs exhibited stability in DBS samples for at least 30 days at room temperature, confirming proper handling and storage of the DBS samples. A 3 mm diameter disc punched from a DBS produced accurate results for all target drugs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The optimized method provided a time- and cost-effective solution, showing a strong correlation between drug concentrations in whole blood, thereby supporting the suitability of DBS sampling as a promising and advanced method for antiepileptic drug monitoring.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"39 16","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/rcm.10064","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143938830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revisiting In-Gas Transformations of Quinate Conjugates Through the LC-qTOF-MS and Molecular Networking Topology","authors":"Nakisani Babra Moyo,&nbsp;Ntakadzeni Edwin Madala","doi":"10.1002/rcm.10068","DOIUrl":"10.1002/rcm.10068","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>The emergence of computational metabolomics tools such as molecular networking and machine learning–based platforms like SIRIUS has significantly advanced MS-based metabolomics studies. These tools enable rapid metabolite identification by deciphering complex fragmentation patterns and chemical transformations occurring during mass spectrometry analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In this study, methanolic extracts of <i>Viscum combreticola</i>, a plant recently shown to contain a rich composition of cinnamic acid–quinates conjugates, were analyzed using the LC-qTOF-MS in combination with a molecular networking approach to explore the chemical complexity of quinate conjugates.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Findings of this study through molecular networking topology revealed that quinic acid undergoes a series of in-gas chemical transformations, including dehydration (–H₂O) and decarboxylation (–CO₂). These transformations yield unique product ions, some of which are associated with other organic acids, such as isocitric acid. By employing the MS² search option on the GNPS2 platform, molecules exhibiting these product ions were readily identified in this study. Therefore, highlighting the potential of this function in GNPS2 for tracing unique fragmentation patterns synonymous with certain molecules that can be used to confirm their identity visually.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The MS² search function can aid in the discovery of new compounds containing the diagnostic ions of interest that could otherwise be easily missed with manual annotation. This study presents a potential validation approach of looking at multiple product ions to confirm the identity of a molecule, particularly in the presence of other compounds with similar fragmentation pathways or shared fragment ions.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"39 16","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/rcm.10068","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143925919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Onsite Analysis of Complex Samples Using Miniature Mass Spectrometry With Direct Sampling and Ionization","authors":"Yiming Zou,&nbsp;Xuan Liu,&nbsp;Linzhi Du,&nbsp;Jiafan Yang,&nbsp;Zhishuang Liu,&nbsp;Zicheng Yuan,&nbsp;Lina Liu,&nbsp;Bicheng Yang,&nbsp;Zhe Shao,&nbsp;Igor A. Popov,&nbsp;Bin Hu","doi":"10.1002/rcm.10056","DOIUrl":"10.1002/rcm.10056","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>Miniature mass spectrometry (mini-MS) is a powerful method for onsite analysis of complex. The onsite sampling/ionization approach is critical in rapid screening of complex samples by mini-MS in field environments. Therefore, new development of direct ionization/sampling method coupled with mini-MS is needed for direct sample analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A direct sampling/ionization kit using wooden tip or plant tissue was developed to couple with mini-MS for onsite analysis of complex samples. Targeted analytes were detected and identified by mini-MS/MS.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Various raw samples including powders, liquid, and viscous samples were directly sampled and ionized using wooden tip. Direct pesticide detection in juice was used to further evaluate the analytical performances of the newly developed wooden tip kit, obtaining excellent sensitivity, reproducibility, and quantitation capacity for direct sample analysis. Furthermore, direct tissue analysis was also successful demonstrated by direct ionization, showing a fast, simple, and efficient method for onsite analysis of biological tissue.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Overall, our data showed that this direct sampling/ionization method using wooden tip or plant tissue coupled with mini-MS approach is simple, fast, and sensitive method for onsite direct analysis of raw samples in field environments.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"39 16","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143925918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-Plug Filtration Purification Combined With Gas Chromatography–Tandem Mass Spectrometry for the Detection of 107 Pesticides in Livestock and Poultry Meat","authors":"Xuwei Guo,&nbsp;Youzhi Su,&nbsp;Jun Liu,&nbsp;Hongqin Lei,&nbsp;Fang Li,&nbsp;Yanmei Li","doi":"10.1002/rcm.10062","DOIUrl":"10.1002/rcm.10062","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>With the global rise in foodborne diseases, excessive pesticide residues in livestock and poultry meat have emerged as a critical food safety issue. China is a large consumer of livestock and poultry meat, and the traditional national method standard can only detect a few or one type of pesticide in these meats. Establishing a method for the simultaneous detection of multiple types of pesticides in these meats can effectively protect people's health and safety.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The extraction process was done using acetonitrile direct extraction, followed by clean-up through multi-plug filtration. Separation was achieved using gas chromatography on an HP-5MS capillary column with a programmed temperature gradient, and detection was performed via mass spectrometry in multiple reaction monitoring modes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The linear ranges of 107 analytes exhibited strong correlation coefficients, all exceeding 0.9901, with limits of quantification spanning from 1.0 to 15.2 μg/kg. The average recoveries, inter-day relative standard deviations (inter-day RSDs), and intra-day relative standard deviations (intra-day RSDs) of the 94 analytes ranged from 70.2% to 120.0%, 4.0% to 16.0%, and 4.5% to 16.8%, respectively, at the three different spiked levels.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>A multi-plug filtration purification combined with gas chromatography-tandem mass spectrometry method was successfully applied to the detection of 107 pesticide residues in livestock and poultry meat, which is simple, reliable, and reproducible. It provides a comprehensive solution for the detection of pesticide residues in livestock and poultry meat.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"39 16","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143925917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of MALDI Matrices and Their Preparation for the MALDI-TOF MS Analysis of Oligonucleotides","authors":"Susanne Dietrich,&nbsp;Anja Dollinger,&nbsp;Andreas Wieser,&nbsp;Christoph Haisch","doi":"10.1002/rcm.10061","DOIUrl":"10.1002/rcm.10061","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>The reproducibility of the analysis of oligonucleotides using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) remains a significant challenge. This is mainly attributed to factors such as the choice and application of the matrix, as well as the inhomogeneity of the sample spots. Although previous studies have explored various parameters, such as ionic and sugar-based matrices, and different spotting methods, none have comprehensively integrated these factors into a unified approach.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Various matrices for the analysis of oligonucleotides and the effects of diverse variables, including different matrices, solvent compositions, additives, and application techniques, on the analytical performance of these matrices were investigated. Mass spectrometry analysis was conducted by a MALDI-TOF MS in linear negative mode.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Our research systematically evaluates the combined effects of diverse variables to enhance the analytical performance of MALDI-TOF MS in oligonucleotide analysis. We focused on the standard deviation of mass-to-charge ratios and the signal-to-noise (S/N) ratios. Out of 48 samples, only 19 met the S/N criteria, which is that the signals must be detectable over the whole mass range of interest (4–10 kDa).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The ionic matrix 6-aza-2-thiothymine (ATT) with 1-methylimidazole resulted consistently in a reduced standard deviation and achieved high mass precisions. Additionally, we observed that the S/N ratios and mass precision of 3-hydroxypicolinic acid (3-HPA) varied significantly depending on the solvent composition and the presence of additives.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"39 15","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/rcm.10061","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143919453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating Intestinal Biotransformation and Gut Microbiota to Uncover the Influence of Tongfu Xiexia Decoction in Rats With Constipation","authors":"Yu-Han Piao,&nbsp;Jing Luo,&nbsp;Tao Ren,&nbsp;Ao Wang,&nbsp;Yu-Yun Li,&nbsp;Li-Jia Pan,&nbsp;Xin-Ze Li,&nbsp;Fang-Tong Li,&nbsp;Yu-Wen Bao,&nbsp;Fei Zheng,&nbsp;Hao Yue","doi":"10.1002/rcm.10065","DOIUrl":"10.1002/rcm.10065","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>Tongfu Xiexia Decoction (TFXXD), a traditional Chinese medicine formula, comprises six herbs: semen raphani, Rehmannia glutinosa, rhubarb, <i>Magnolia officinalis</i>, trifoliate orange, and mirabilite. The aim of this study was to investigate the effects of TFXXD on its biotransformation and microbial abundance in the gut of constipated rats.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Ultrahigh-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to analyze the biotransformation of TFXXD in the colonic contents, and 16S rRNA sequencing was used to assess the structure and diversity of the gut microbiota across various rat groups.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>We identified and analyzed 25 biotransformations of TFXXD in the colonic contents, which undergo various reactions such as deglycosylation, ring opening, and hydration in intestinal bacteria, with 14 originating from trifoliate orange, six from rhubarb, three from Rehmannia glutinosa, one from Semen raphani, and one from <i>M. officinalis</i>. 16S rRNA analysis revealed that TFXXD significantly enhanced the relative abundance of beneficial bacteria, such as <i>Lactobacillus</i> and <i>Bacteroides</i> while significantly reducing <i>Oscillospira</i> abundance. Moreover, TFXXD considerably affected the carbohydrate and amino acid metabolism. Correlation analyses revealed a significant negative correlation between <i>Bacteroides</i> and ACH (Acetylcholine), NO (nitric oxide), D-Lac (D-Lactate), IL-6 (Interleukin-6), TNF-α (tumor necrosis factor-α), and IL-1β (Interleukin-1β) and a significant positive correlation between <i>Bacteroides</i>, hesperetin, and rhein.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>In conclusion, our findings indicate that TFXXD can modulate the structure and diversity of the gut microbiota and enhance the metabolic balance in constipated rats.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"39 15","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143914260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Aerobic Salivary Microorganisms in Patients With Oral Squamous Cell Carcinoma Using MALDI-TOF MS: Preliminary Findings From a Pilot Study","authors":"Milena da Silva Moreira,&nbsp;Giovanna Schmitz,&nbsp;Mariana de Sá Alves,&nbsp;Maria Anita Mendes,&nbsp;Mônica Ghislaine Oliveira Alves,&nbsp;Levy Anderson César Alves,&nbsp;Meriellen Dias,&nbsp;Celso Muller Bandeira,&nbsp;Gabrielle Luana Jimenez Teodoro Nepomuceno,&nbsp;Herculano da Silva Martinho,&nbsp;Janete Dias Almeida","doi":"10.1002/rcm.10063","DOIUrl":"10.1002/rcm.10063","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>Oral squamous cell carcinoma (OSCC) is a multifactorial disease, and emerging evidence links the oral microbiome to its development. Rapid, noninvasive identification of salivary microorganisms may offer novel diagnostic and prognostic insights into oral cancer.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Salivary microbiota from OSCC patients and healthy individuals were analyzed using MALDI Biotyper. Saliva samples were cultured, and microbial identification was performed based on protein spectral profiles using an UltrafleXtreme MALDI-TOF mass spectrometer.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Thirteen OSCC patients (mean age 55 ± 11 years; 69% male) and nineteen healthy controls (mean age 55 ± 10 years; 79% male) were analyzed. Distinct microbial profiles were observed in OSCC patients, including pathogenic species previously associated with carcinogenesis, suggesting potential biomarkers for oral cancer.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The MALDI Biotyper is an effective, noninvasive tool for identifying salivary microbiota. Its application may support early diagnosis and prognosis of OSCC, reinforcing the significance of the oral microbiome in cancer etiology.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"39 15","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/rcm.10063","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143905096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Mineral Element Concentrations in Plasma Samples and Their Correlation to HIV/AIDS","authors":"Long-Hua Zhou,&nbsp;Chang-Hua Feng,&nbsp;Xin-Chen Lin,&nbsp;Wei-Hua Huang,&nbsp;Yue Wu,&nbsp;Yong-Jiu Jin,&nbsp;Yang-Yun Zheng,&nbsp;Shuang-Li Guo,&nbsp;Xiang Xia,&nbsp;Wei-Hua Wang,&nbsp;Xiao-Tong Qi,&nbsp;Hui Li","doi":"10.1002/rcm.10060","DOIUrl":"10.1002/rcm.10060","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>The main purpose of this study was to contribute to the development of new strategies for prevention and management, ultimately improving the diagnosis, treatment, and overall well-being of affected individuals and communities.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Plasma samples were collected from 120 HIV-positive individuals and 120 healthy controls. The concentrations of the elements were determined by ICP-MS and ICP-OES. Statistical analysis was performed with the Kolmogorov–Smirnov normality test, the Mann–Whitney <i>U</i> test and the Spearman's rank correlation test.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The study found that HIV/AIDS patients had lower concentrations of Na, Mg, Ca, Fe, Zn, Se, Rb, and Sr compared to the healthy group (<i>p</i> = 0.000 &lt; 0.05). The concentration of I was also lower in HIV/AIDS patients (<i>p</i> = 0.025 &lt; 0.05). However, there was no significant difference in Cu concentrations between the two groups (<i>p</i> = 0.810 &gt; 0.05). HIV/AIDS patients had higher concentrations of Ba (<i>p</i> = 0.000 &lt; 0.05). Significant positive correlations were observed between several element pairs in HIV/AIDS patients, including Na-Ca, Na-Se, Mg-Ca, Mg-Zn, Mg-Sr, Ca-Zn, Ca-Se, Ca-Sr, Fe-Rb, and Sr-Ba at level 0.01, and Na-Zn, Mg-Rb, Mg-Ba, Ca-Ba, and Fe-I at level 0.05. Negative correlations were found between Na-I, Ca-I, Fe-Cu, and Cu-Rb concentrations in HIV/AIDS patients.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>In summary, by identifying and addressing imbalances or deficiencies in mineral elements, healthcare providers can optimize patient care and support better health outcomes. This research may lead to the development of interventions targeting mineral imbalances and optimizing immune function in individuals with HIV/AIDS.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"39 15","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143904932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}