Fentanyl and Fentanyl Analog Screening Using ASAP-MS With LiveID Confirmation

IF 1.8 3区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Rapid Communications in Mass Spectrometry Pub Date : 2025-01-28 DOI:10.1002/rcm.9994

Karen A. Reyes Monroy, Rachel Koerber, Guido F. Verbeck

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引用次数: 0

Abstract

Rationale

Fentanyl and fentanyl analogs continue to pose a serious threat to the public health. The vast number of fentanyl analogs emerging on the black-market call for optimized analytical methods for the detection, analysis, and characterization of these extremely dangerous drugs.

Methods

Atmospheric pressure solids analysis probe (ASAP) mass spectrometry was used for the rapid analysis of 250 synthetic opioid standards, including 211 fentanyl analogs, 32 non-fentanyl related opioids, and 8 fentanyl precursors. Four cone voltages (5, 15, 35 and 50 V) were used to obtain fingerprint data for each opioid reference sample.

Results

The generated and processed mass spectra of the 250 synthetic opioids analyzed was utilized to create an ASAP⁺ database that contains the largest compendium of mass spectra for fentanyl analogs. The built library was integrated into LiveID software, enabling real-time analyte identification. The efficacy of the software's ability to identify fentanyl analogs in a sample utilizing the spectral library was tested by analyzing five blind and four reaction mixtures. The correct identity of these nine samples was all within the top three ranked matches.

Conclusion

We demonstrate how the RADIAN ASAP, alongside a real-time sample recognition software, can be utilized as a presumptive tool for the screening of fentanyl analogs within samples in question, making it a promising alternative to some of the most commonly used analytical screening techniques.

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用asp - ms筛选芬太尼和芬太尼类似物

理由：芬太尼和芬太尼类似物继续对公众健康构成严重威胁。大量芬太尼类似物出现在黑市上，需要优化分析方法来检测、分析和表征这些极其危险的药物。方法：采用常压固体分析探针（ASAP）质谱法对250种合成阿片类药物标准品进行快速分析，其中包括211种芬太尼类似物、32种非芬太尼相关阿片类药物和8种芬太尼前体。采用5、15、35和50 V四种锥体电压获取每个阿片类药物参考样品的指纹数据。结果：利用生成和处理的250种合成阿片类药物的质谱建立了ASAP+数据库，该数据库包含最大的芬太尼类似物质谱汇编。构建的库集成到LiveID软件中，实现实时分析物识别。该软件利用光谱库在样品中识别芬太尼类似物的能力的有效性通过分析五种盲反应和四种反应混合物进行了测试。这9个样本的正确身份都在排名前三的匹配范围内。结论：我们展示了RADIAN ASAP如何与实时样本识别软件一起，作为一种假定工具，用于筛选芬太尼类似物中的问题样品，使其成为一些最常用的分析筛选技术的有希望的替代方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Rapid Communications in Mass Spectrometry 化学-分析化学

CiteScore

4.10

自引率

5.00%

发文量

219

审稿时长

2.6 months

期刊介绍： Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.