Fentanyl and Fentanyl Analog Screening Using ASAP-MS With LiveID Confirmation

IF 1.8 3区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Rapid Communications in Mass Spectrometry Pub Date : 2025-01-28 DOI:10.1002/rcm.9994

Karen A. Reyes Monroy, Rachel Koerber, Guido F. Verbeck

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引用次数: 0

Abstract

Rationale

Fentanyl and fentanyl analogs continue to pose a serious threat to the public health. The vast number of fentanyl analogs emerging on the black-market call for optimized analytical methods for the detection, analysis, and characterization of these extremely dangerous drugs.

Methods

Atmospheric pressure solids analysis probe (ASAP) mass spectrometry was used for the rapid analysis of 250 synthetic opioid standards, including 211 fentanyl analogs, 32 non-fentanyl related opioids, and 8 fentanyl precursors. Four cone voltages (5, 15, 35 and 50 V) were used to obtain fingerprint data for each opioid reference sample.

Results

The generated and processed mass spectra of the 250 synthetic opioids analyzed was utilized to create an ASAP⁺ database that contains the largest compendium of mass spectra for fentanyl analogs. The built library was integrated into LiveID software, enabling real-time analyte identification. The efficacy of the software's ability to identify fentanyl analogs in a sample utilizing the spectral library was tested by analyzing five blind and four reaction mixtures. The correct identity of these nine samples was all within the top three ranked matches.

Conclusion

We demonstrate how the RADIAN ASAP, alongside a real-time sample recognition software, can be utilized as a presumptive tool for the screening of fentanyl analogs within samples in question, making it a promising alternative to some of the most commonly used analytical screening techniques.

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来源期刊

Rapid Communications in Mass Spectrometry 化学-分析化学

CiteScore

4.10

自引率

5.00%

发文量

219

审稿时长

2.6 months

期刊介绍： Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.