Ambient Ionization Mass Spectrometry for Fatty Acids Composition Investigation of Natural Lipids: A Multidisciplinary Workshop.

IF 1.8 3区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Rapid Communications in Mass Spectrometry Pub Date : 2025-01-23 DOI:10.1002/rcm.9985

Stanislav I Pekov

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引用次数: 0

Abstract

Rationale: Teaching mass spectrometry essentials is usually connected with one of the basic courses for undergrads. Thus, specific previous knowledge is required from students. However, the necessity of teaching mass spectrometry essentials to students of different academic specializations and multidisciplinary groups can arise in every academic group. A specific workshop is needed to address such a demand.

Methods: The presented workshop consisted of several thematic parts: assembling an ambient ionization ion source using improvised materials, preparing biological samples for analysis, data acquisition, and interpretation of data to solve a simple problem from the real world.

Results: The first part of the work consisted of assembling an ambient ionization setup and obtaining mass spectra of substances from standard solutions, natural mixtures, and biological fluids such as saliva. The second half of the workshop consists of analyzing the composition of fatty acids of natural and artificial fats using the same ion source. The identification of oils is a simple model problem that makes the workshop attractive for attendees with different backgrounds.

Conclusions: The workshop provides students with practical skills that are highly valuable in fundamental and applied mass spectrometry. Students familiarize themselves with the basic concepts, instrument use, and mass spectra interpretation. They achieve basic hands-on experience in experimentation procedures and the practice of using mass spectrometry to solve problems related to real life.

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环境电离质谱法用于天然脂类脂肪酸组成的研究：一个多学科研讨会。

理据：质谱基本知识的教学通常与本科生的一门基础课程相联系。因此，需要学生具备具体的前期知识。然而，对不同学术专业和多学科群体的学生教授质谱基本知识的必要性在每个学术群体中都可能出现。需要一个专门的讲习班来满足这种需求。方法：本次研讨会包括几个主题部分：使用临时材料组装环境电离离子源，准备用于分析的生物样品，数据采集和数据解释，以解决现实世界中的一个简单问题。结果：工作的第一部分包括组装一个环境电离装置，并从标准溶液、天然混合物和生物流体（如唾液）中获得物质的质谱。工作坊的后半部分包括分析使用相同离子源的天然脂肪和人工脂肪的脂肪酸组成。油的识别是一个简单的模型问题，使研讨会吸引了来自不同背景的与会者。结论：本课程为学生提供了在质谱基础和应用方面极具价值的实践技能。学生熟悉基本概念、仪器使用和质谱解释。他们获得了实验程序的基本实践经验，并使用质谱法解决与现实生活有关的问题。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Rapid Communications in Mass Spectrometry 化学-分析化学

CiteScore

4.10

自引率

5.00%

发文量

219

审稿时长

2.6 months

期刊介绍： Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.