The Japanese journal of experimental medicine最新文献_第6页

Thermal injury-induced non-specific resistance to fatal Pseudomonas aeruginosa burn-infection in mice. 热损伤诱导小鼠对致命铜绿假单胞菌烧伤感染的非特异性抗性。

The Japanese journal of experimental medicine Pub Date : 1989-10-01

M Pinto, T Zehavi-Willner

{"title":"Thermal injury-induced non-specific resistance to fatal Pseudomonas aeruginosa burn-infection in mice.","authors":"M Pinto, T Zehavi-Willner","doi":"","DOIUrl":"","url":null,"abstract":"Nonlethal thermal injury in mice results in rapid death by immediate injection of 10(3) viable P. aeruginosa in the skin of the burn sites. Resistance to the lethal burn combined with P. aeruginosa infection developed 24 h after initial thermal injury and reached maximal effect 7 days later; it then continued for at least 21 days. The optimal survival was achieved when the first thermal injury was made for 7 seconds at 350 degrees C. Increased resistance, but for a short period could also be obtained by injection of lipopolysaccharide (LPS) 1-4 days prior to the burn-P. aeruginosa infection. However, when the LPS was injected immediately after the burn-infection, the lethal effect was increased. The induction of late protection after thermal injury and bacterial infection was demonstrated with P. aeruginosa organisms only. Under similar schedule of thermal injury resistance was not induced by infection with Semliki forest virus. On the contrary viral infection increased the susceptibility of burned mice to a fatal outcome. Immune or natural antibodies were not elevated in the sera of post burn mice. Furthermore, delayed type hypersensitivity response, as evaluated by a footpad weight assay was inhibited and this inhibition persisted at least for 7 days post burn. The thymus weight and its lymphoid cell content in thermally injured mice decreased significantly 7 days post burn, whereas the weight of the spleen increased and it contained fewer lymphocytes per gram tissue. We suggest that endotoxin entering the systemic circulation post-burn might be one of the factors contributing to the early sensitivity and the late protection against the fatal P. aeruginosa burn-infection.","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13703040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential usefulness of a cultured glioma cell line induced by Rous sarcoma virus in B10.A mouse as an immunotherapy model. 劳斯肉瘤病毒诱导的B10神经胶质瘤细胞系培养的潜在用途。小鼠作为免疫治疗模型。

The Japanese journal of experimental medicine Pub Date : 1989-10-01

K Sakamoto, H Hoshino, Y Kiuchi, G Nakano, Y Nagamachi

{"title":"Potential usefulness of a cultured glioma cell line induced by Rous sarcoma virus in B10.A mouse as an immunotherapy model.","authors":"K Sakamoto, H Hoshino, Y Kiuchi, G Nakano, Y Nagamachi","doi":"","DOIUrl":"","url":null,"abstract":"A cultured glioma cell line, SR-B10.A, which was derived from a brain tumor induced in an adult female B10.A mouse by Rous sarcoma virus (RSV), has been established. The morphological appearance of the tumor produced by s.c. inoculating SR-B10.A cells was analogous to an astrocytoma of human glioma. Glial fibrillary acidic protein as well as S-100 protein was positive in these SR-B10.A tumor cells. A population doubling time of the cultured cells was 18.5 hours. Chromosomal analysis revealed a defect in one of the sex chromosomes. Integration of RSV genome was proven to be positive in SR-B10.A cells. It was possible to generate cytotoxic effector cells in the syngeneic B10.A mouse against SR-B10.A. The tumor-bearing syngeneic hosts harbored a suppressor activity in the splenocytes. Although recombinant human tumor necrosis factor (rH-TNF) had no growth inhibitory effect on the SR-B10.A cells in vitro, the s.c. implanted and growing tumor regressed when rH-TNF was administered intratumorally several times. In addition, this anti-tumor effect was completely abrogated when the host mice were treated with wholebody x-ray irradiation prior to the tumor cells inoculation. In contrast, neither rH-TNF (i.v.) nor cyclophosphamide (i.p.) induced the regression of SR-B10.A, indicating that efficacy of the locally administered rH-TNF is dependent on the host immune mechanism. These results suggest that SR-B10.A is a potentially useful tumor model in evaluating efficacy of immunomodulators.","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13702461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of atrial natriuretic peptide on sodium homeostasis in experimental renal failure. 心房利钠肽在实验性肾衰竭钠稳态中的作用。

The Japanese journal of experimental medicine Pub Date : 1989-10-01

K Nitta, M Naruse, T Sanaka, K Tsuchiya, N Sugino

{"title":"Role of atrial natriuretic peptide on sodium homeostasis in experimental renal failure.","authors":"K Nitta, M Naruse, T Sanaka, K Tsuchiya, N Sugino","doi":"","DOIUrl":"","url":null,"abstract":"In a study of the role of atrial natriuretic peptide (ANP) in sodium homeostasis in experimental renal failure, we found that a infusion of ANP at 0.25 microgram/min for 15 min produced an increase in the glomerular filtration rate (GFR) and fractional excretion of sodium (FENa) in five-sixth nephrectomized (5/6 Nx) rats. Renal vascular resistance (RVR) was lower during the base-line period and did not change after the administration of 100 ng/ml ANP to isolated perfused kidney (IPK) from adriamycin-treated rats. Furthermore, fractional excretion of ANP (FEANP) by IPK decreased in kidneys from adriamycin-treated rats as compared to that in kidneys from control rats. Finally, after 5/6 Nx, levels of plasma immunoreactive ANP (ir-ANP) gradually increased but excretion of water and sodium did not change during normal intake of sodium. The increase in levels of ir-ANP was accompanied by an increase in the rates of excretion of water and sodium was observed 2 days later but these rates returned to the base-line values after 2 weeks. These findings suggest that ANP plays an important role in the adjustment of acute changes in the volume of extracellular fluid during experimental renal failure.","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13677722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructural observations on damage to schistosomula of Schistosoma japonicum by mouse neutrophils in vitro. 小鼠中性粒细胞对日本血吸虫体外损伤的超微结构观察。

The Japanese journal of experimental medicine Pub Date : 1989-10-01

Y Irie, K Yasuraoka

引用次数: 0

Immunohistochemical study on the excretion of a drug (methamphetamine) by salivary glands. 唾液腺对药物(甲基苯丙胺)排泄的免疫组织化学研究

The Japanese journal of experimental medicine Pub Date : 1989-10-01

A Kajitani, M Kaiho, Y Okada, I Ishiyama

引用次数: 0

Schistosomiasis on Bohol Island, Philippines, with special emphasis on the successful discovery of new habitats of the vector snail, Oncomelania quadrasi, and area-wide mollusciciding. 菲律宾保和岛的血吸虫病，特别强调成功发现了病媒蜗牛的新栖息地，并在整个地区进行了灭虫。

The Japanese journal of experimental medicine Pub Date : 1989-08-01

K Yasuraoka, A T Santos, B L Blas, H Tanaka, H Matsuda, Y Irie, H Shimomura, R Pangilinan

引用次数: 0

Antitumor activity of an extract of Cordyceps sinensis (Berk.) Sacc. against murine tumor cell lines. 冬虫夏草提取物的抗肿瘤活性研究Sacc。抗小鼠肿瘤细胞系。

The Japanese journal of experimental medicine Pub Date : 1989-08-01

J Yoshida, S Takamura, N Yamaguchi, L J Ren, H Chen, S Koshimura, S Suzuki

{"title":"Antitumor activity of an extract of Cordyceps sinensis (Berk.) Sacc. against murine tumor cell lines.","authors":"J Yoshida, S Takamura, N Yamaguchi, L J Ren, H Chen, S Koshimura, S Suzuki","doi":"","DOIUrl":"","url":null,"abstract":"A warm water-extract (ECS) prepared from dried Cordyceps sinensis (Berk.) Sacc., a Chinese traditional medicine, was tested for antitumor activity in vivo and in vitro. Ehrlich ascites carcinoma cells (EAC), allogeneic to ICR mice and Meth A fibrosarcoma (Meth A), syngeneic to BALB/c mice were used as the target tumor cell lines. Mice were inoculated i.p. with 1 x 10(6) EAC or 1 x 10(5) Meth A on Day 0, and ECS or saline (control) was injected i.p. to the mice from Day 1 to Day 4. ECS-treatment increased the median survival time of the allogeneic mice inoculated with EAC to 316% of the control. Eight of the 10 ECS-treated mice survived on the 60th day (Day 60) after EAC implantation. ECS-treatment also increased the median survival time of the syngeneic mice inoculated with Meth A to 312% of the control. Half of the ECS-treated mice survived on Day 60. On the other hand, no cytotoxic effect of ECS was found on either EAC or Meth A in vitro. The antitumor effect of ECS seen in the allogeneic mice was significantly reduced when the mice received whole body X-irradiation (5 Gy) before EAC implantation. These results suggest that the antitumor effect of ECS may be mediated through its immunomodulating action.","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13743449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of ciclosporin on the renal histopathological aspects in (NZB x NZW)F1 mice. 环孢素对(NZB x NZW)F1小鼠肾脏组织病理学的影响。

The Japanese journal of experimental medicine Pub Date : 1989-08-01

M Nishikawa, M Nagata, M Takeda, T Sato, T Sanaka, N Sugino

引用次数: 0

Enzyme immunoassay (ELISA) for the detection of anticarbohydrate antibodies in rheumatic fever and rheumatic heart disease. 酶免疫分析法(ELISA)用于风湿热和风湿性心脏病抗碳水化合物抗体的检测。

The Japanese journal of experimental medicine Pub Date : 1989-08-01

V Wahi, N K Ganguly, R Sehgal, I S Anand, N Kaur, R N Chakravarti, P L Wahi

{"title":"Enzyme immunoassay (ELISA) for the detection of anticarbohydrate antibodies in rheumatic fever and rheumatic heart disease.","authors":"V Wahi, N K Ganguly, R Sehgal, I S Anand, N Kaur, R N Chakravarti, P L Wahi","doi":"","DOIUrl":"","url":null,"abstract":"Antibodies to group A streptococcal polysaccharide were estimated in the following groups of patients: (I) Patients with uncomplicated streptococcal pharyngitis: 10 patients, followed up for 3 months. (II) Patients with acute rheumatic fever: 8 patients with first attack followed up for one year. (III) Patients with reactivated rheumatic heart disease: 10 patients, followed up for one year. (IV) Patients with chronic rheumatic heart disease: followed up for one year. (V) normal controls without any history of sore throat/fever/vaccination/rheumatic disease: 10 patients followed up for one year. Group (I) patients did not show any significant elevation in anticarbohydrate antibodies by both ELISA and RIA. In the case of (III) and (IV), antibody levels were significantly higher as compared to group (V) and remained so till one year of follow up. In group (II) patients there was no significant rise in antibody levels. There was a good correlation between the ELISA and RIA used to detect the antibody levels. These findings suggest that the use of ELISA to detect anticarbohydrate antibody can be of help in diagnosing cases of rheumatic heart disease (both acute and chronic RHD).","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13830139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of monoclonal antibodies against nuclear antigens in a DNA affinity purified protein fraction from chick embryo extract. 鸡胚提取物DNA亲和纯化蛋白片段中核抗原单克隆抗体的制备。

The Japanese journal of experimental medicine Pub Date : 1989-08-01

S Matsuhashi, Y Arai, T Watanabe, K Hori

引用次数: 0