发布求助
文献互助 智能选刊 最新文献

The Japanese journal of experimental medicine最新文献

筛选
英文 中文
erbB-2 gene expression in colorectal cancer. erbB-2基因在结直肠癌中的表达。
The Japanese journal of experimental medicine Pub Date : 1990-12-01
G J Tsioulias, T Muto, Y Morioka, T Yamamoto, S Mori
{"title":"erbB-2 gene expression in colorectal cancer.","authors":"G J Tsioulias,&nbsp;T Muto,&nbsp;Y Morioka,&nbsp;T Yamamoto,&nbsp;S Mori","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The expression of the erbB-2 gene product was studied immunohistochemically on 38 normal colonic mucosae, 14 adenomas and 44 colon cancers, with the use of two anti-erbB-2 antibodies, a rabbit polyclonal antibody specific for the intracellular domain and a mouse monoclonal antibody specific for the extracellular domain. Normal mucosae and adenomas were not stained. Five cases (11%) of colon cancer were positive with the polyclonal anti-erbB-2 antibody, while only one case was positive with the monoclonal antibody. Most of the positive cases were in Dukes C stage. The rare overexpression of the erbB-2 protein in colon cancer seems to indicate a minor role for the gene in colorectal tumorigenesis.</p>","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13140044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization and change of phospholipids in the aorta of Watanabe hereditable hyperlipidemic rabbit. 渡边遗传性高脂血症家兔主动脉磷脂的特征及变化。
The Japanese journal of experimental medicine Pub Date : 1990-12-01
A Hara, T Taketomi
{"title":"Characterization and change of phospholipids in the aorta of Watanabe hereditable hyperlipidemic rabbit.","authors":"A Hara,&nbsp;T Taketomi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The characterization and change of lipids in aorta with the progression of atherosclerosis were elucidated in Watanabe hereditable hyperlipidemic (WHHL) rabbit, an animal model for human familial hypercholesterolemia as compared with those of normal rabbits. The amount of total phospholipids in the aorta of WHHL rabbit increased about 6-fold that of normal level. Sphingomyelin showed the largest increase and phosphatidylcholine the second in the aorta of WHHL rabbit. The fatty acid composition of sphingomyelin was characteristic of higher content of unsaturated fatty acids. It was suggested that the specific increase in both sphingomyelin and its unsaturated fatty acid content might be ascribed to regulation mechanism to recover the membrane fluidity which was reduced by the marked accumulation of cholesterol in the aorta of WHHL rabbit. Glycerophospholipids in the aorta of WHHL rabbit also contained more unsaturated fatty acids than those in normal ones. It was suggested that the higher amounts of unsaturated fatty acids of glycerophospholipids might be derived from those of serum lipoproteins and that they also might help to restore or recover membrane fluidity in the cholesterol-accumulated aorta of WHHL rabbit. Phosphatidylcholine in the aorta of WHHL rabbit contained a large amount of alkylacyl type-homologue (25-fold normal level) which was a precursor of platelet-activating factor (PAF). The increase of lysophosphatidylcholine, a potent chemotactic factor for monocyte, was also observed in WHHL rabbit. The increase of these chemical mediators indicated severe pathologic state in the atherosclerotic lesions of the aorta of WHHL rabbit.</p>","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13251690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protein extractabilities from the erythrocyte membranes in patients with chorea-acanthocytosis. 舞蹈病-棘细胞增多症患者红细胞膜蛋白的提取。
The Japanese journal of experimental medicine Pub Date : 1990-12-01
K Asano, M Oshima
{"title":"Protein extractabilities from the erythrocyte membranes in patients with chorea-acanthocytosis.","authors":"K Asano,&nbsp;M Oshima","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13305779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Suppression of footpad reaction in mice by anthralin. 炭疽素对小鼠脚垫反应的抑制作用。
The Japanese journal of experimental medicine Pub Date : 1990-12-01
M Tabara, M Watanabe
{"title":"Suppression of footpad reaction in mice by anthralin.","authors":"M Tabara,&nbsp;M Watanabe","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In the preceding papers, we showed that the treatment of BALB/c mice with 12-O-tetradecanoylphorbol 13-acetate (TPA), a typical tumor promoter, suppressed footpad reaction (FPR) against sheep red blood cells (SRBC) temporarily but that this effect became lasting when TPA was administered to mice which had been treated with 7,12-dimethyl[a]-anthracene (DMBA), a typical tumor initiator, and that the effect of DMBA and TPA was caused by the induction of antigen-nonspecific T suppressor cells. In this work, we studied the effect of anthralin, a tumor promoter which has not the structure of phorbol ester, on FPR of BALB/c mice against SRBC. Painting of anthralin (80 nmol) suppressed FPR continuously (more than 7 days) unlike that of TPA. However, when anthralin was administered for 7 days following the treatment with 400 nmol of DMBA, the suppressive effect could be transferred with Thy-1 and Lyt-2 positive spleen cells whereas the suppressive effect by the painting of anthralin only for 7 days could not be transferred with the spleen cells.</p>","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13283629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Aggregated distribution of infective spots composed of Leptotrombidium pallidum, highly prevalent with Rickettsia tsutsugamushi, demonstrated by sentinel voles, Microtus montebelli, on the ground. 由白衣细体组成的感染点聚集分布，与恙虫病立克次体高度流行，由地面上的蒙特贝利田鼠哨兵田鼠证明。
The Japanese journal of experimental medicine Pub Date : 1990-12-01
M Takahashi, M Murata, K Machida, E Hori, A Kawamura, H Tanaka
{"title":"Aggregated distribution of infective spots composed of Leptotrombidium pallidum, highly prevalent with Rickettsia tsutsugamushi, demonstrated by sentinel voles, Microtus montebelli, on the ground.","authors":"M Takahashi,&nbsp;M Murata,&nbsp;K Machida,&nbsp;E Hori,&nbsp;A Kawamura,&nbsp;H Tanaka","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In the epidemiological surveys on scrub typhus at Chichibu City near Tokyo, an area, 350 m by 35 m, in the Hitsujiyama Park was found to be heavily infested with Rickettsia tsutsugamushi (Rt). Distribution of trombiculid mites and their infection rates in this area were studied using sentinel animals, 48 Microtus montebelli and 10 ddY mice. The surveys were conducted 6 times in the autumn in 1985 and 1986. At the first 2 surveys, 16 animals were placed at random, and 4 restricted areas were identified as highly populated with trombiculid mites. For the detailed survey, each of 4 areas was divided into 2 m x 2 m quadrats to settle a sentinel animal to each. A total of mites collected by all sentinel animals was 331 Leptotrombidium pallidum, 175 L. fuji and 16 Gahrliepia saduski. Almost all mites were collected by M. montebelli, except only 1 L. fuji in 10 mice. From a M. montebelli in a quadrat, 157 L. pallidum were recovered, whereas the number/vole was 0 to 24 in the others. L. fuji was also highly aggregated at 2 sites. Rt was detected from individual mites by avidin-biotin immunofluorescence or isolated by the mouse passage from individual or pooled mites. Only Karp strain of Rt was detected or isolated from L. pallidum at a ratio of 31/286 (10.8%). No Rt was found from L. fuji or G. saduski. The infection rate in L. pallidum was especially high in 3 voles at ratios of 6/11, 2/5 and 3/5, respectively. Out of 14 sentinel M. montebelli with infected L. pallidum, 12 (85.7%) were infected with Rt. It was concluded that L. pallidum was distributed in aggregated clusters to form the mite islands and was infected heavily at the specific sites to make the infective spots.</p>","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13283630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Estrogen and progesterone receptor levels in 7,12-dimethylbenz[a]anthracene-induced rat mammary tumors in each week--check points about experimental studies using DMBA-induced tumors. 7,12-二甲基苯[a]蒽诱导大鼠乳腺肿瘤每周雌激素和孕激素受体水平——dba诱导肿瘤实验研究的检查点
The Japanese journal of experimental medicine Pub Date : 1990-12-01
Y Iino, M Izuo, R Horiuchi
{"title":"Estrogen and progesterone receptor levels in 7,12-dimethylbenz[a]anthracene-induced rat mammary tumors in each week--check points about experimental studies using DMBA-induced tumors.","authors":"Y Iino,&nbsp;M Izuo,&nbsp;R Horiuchi","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13283631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Suppression of cytoplasmic Ca2+ response and protein secretion by oxidizing agents. 氧化剂抑制细胞质Ca2+反应和蛋白分泌。
The Japanese journal of experimental medicine Pub Date : 1990-12-01
M Tomono, T Urayama
{"title":"Suppression of cytoplasmic Ca2+ response and protein secretion by oxidizing agents.","authors":"M Tomono,&nbsp;T Urayama","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The influence of the oxidizing agents (H2O2, KO2 and Vitamin K) on the action of vasopressin to guinea pig hepatocytes was investigated from the view-point of cytoplasmic Ca2+ concentration and protein secretion. 10 nmol/l vasopressin brought about increase in prothrombin secretion along with increase in the cytoplasmic Ca2+ concentration compared to the non-stimulation level. The pretreatment of the cells with 1 mumol/l of the oxidizing agents, however, led to suppression of Ca2+ elevation and inhibited the vasopressin-induced prothrombin secretion completely, while no leak if lactic dehydrogenase (LDH), Na+ and K+ were detected. The same results of the inhibition in fibrinogen and albumin secretion were observed. These results suggested a possibility that the oxidizing agents such as the peroxides act on some site of cellular signal transduction system in cell membrane to reduce the cytoplasmic Ca2+ level and to suppress the vasopressin-induced secretion.</p>","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13123315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A new technique of in vitro assay of antifilarials using different life-forms of Acanthocheilonema viteae. 利用不同生命形式的棘条线虫体外测定抗丝虫药的新技术。
The Japanese journal of experimental medicine Pub Date : 1990-12-01
D P Singh, S Misra, R K Chatterjee
{"title":"A new technique of in vitro assay of antifilarials using different life-forms of Acanthocheilonema viteae.","authors":"D P Singh,&nbsp;S Misra,&nbsp;R K Chatterjee","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An approach has been made to develop an in vitro screening system to evaluate antifilarial efficacy of compounds and an effort has been made to establish correlation between in vivo and in vitro screening technique. The in vitro experiments were conducted simultaneously using three life-forms (adult, microfilaria and infective larva) of Acanthocheilonema viteae using five antifilarial agents representing four chemical groups. All the selected antifilarials were known to be active against one or more life-stages of human lymphatic or animal filariids. Diethylcarbamazine and Centperazine showed 100% microfilaricidal and infective larvicidal actions at concentrations of 0.5 and 0.25 mg/ml and 0.5 and 0.0313 mg/ml respectively with no effect on adult worms even at 1 mg/ml. Levamisole was effective against all the three life-stages killing 100% adult worms at 1 mg/ml, infective larvae at 0.0625 mg/ml and microfilariae at 0.0125 mg/ml, while mebendazole exhibited activity only against adult worms (100% at 0.5 mg/ml). Ivermectin killed adult females and microfilariae at 0.063 and 0.5 mg/ml respectively but did not affect infective larvae even up to 1 mg/ml concentration. The study indicated that in vitro screening system can be used for primary screening of potential antifilarial agents provided three life-forms of A. viteae are used simultaneously to avoid false negative results. It would however be more appropriate if a few compounds of a particular chemical class are initially assessed both in vivo and in vitro for validity of subsequent test results in vitro.</p>","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13251689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Long term effects of adult-thymectomy on natural killer activity and spontaneous tumor incidence. 成人胸腺切除术对自然杀伤活性和自发性肿瘤发生率的长期影响。
The Japanese journal of experimental medicine Pub Date : 1990-10-01
A Hussain, H Mayumi, K Taniguchi, K Tanaka, K Nomoto
{"title":"Long term effects of adult-thymectomy on natural killer activity and spontaneous tumor incidence.","authors":"A Hussain,&nbsp;H Mayumi,&nbsp;K Taniguchi,&nbsp;K Tanaka,&nbsp;K Nomoto","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Long term effects of adult-thymectomy on both natural killer (NK) activity and spontaneous tumor incidence were investigated in C57BL/6 mice maintained in specific pathogen free atmosphere. As compared with the age matched sham-thymectomized mice, a substantially low incidence of spontaneous tumour (6%) and a high level of splenic NK activity were noted one and a half year later in C57BL/6 mice thymectomized at 6 weeks of age. In contrast, sham-thymectomized mice showed a high incidence of the spontaneous tumor (57%) associated with a low level of splenic NK activity. These results suggest that NK cells play a critical role to prevent the occurrence of spontaneous tumor in vivo.</p>","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13235480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mono-sulfated glycosphingolipids of human amnion. 人羊膜的单硫酸盐鞘糖脂。
The Japanese journal of experimental medicine Pub Date : 1990-10-01
K Asano, M Oshima
{"title":"Mono-sulfated glycosphingolipids of human amnion.","authors":"K Asano,&nbsp;M Oshima","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Two mono-sulfated glycosphingolipids were isolated from the human amnion. By carbohydrate analysis and acid hydrolysis, the structure of these glycolipids are proposed to be galactosylsulfatide (SM4s) and lactosylsulfatide (SM3). The major fatty acids of SM4s and SM3 were C16:0. The yields of SM4s and SM3 from the amnion were about 340 and 110 pmol/g wet wt, respectively.</p>","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13235482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信