The Japanese journal of experimental medicine最新文献_第2页

Mechanism of protection provided by active immunization with porins in mice challenged with Salmonella typhi. 鼠伤寒沙门菌主动免疫对鼠伤寒沙门菌的保护机制。

The Japanese journal of experimental medicine Pub Date : 1990-10-01

P Sharma, B K Sharma, S Sharma, I J Rawal, S N Saxena, D Panigrahi, N K Ganguly

引用次数: 0

Production of monoclonal antibody against human prostatic adenocarcinoma and its immunohistochemical properties. 抗人前列腺腺癌单克隆抗体的制备及其免疫组织化学性质研究。

The Japanese journal of experimental medicine Pub Date : 1990-10-01

H Matsuki, S Ozono, T Yoshie, K Kubota, Y Hirao, N Konishi, H Hashimoto, M Ohshima, Y Hiasa, E Okajima

{"title":"Production of monoclonal antibody against human prostatic adenocarcinoma and its immunohistochemical properties.","authors":"H Matsuki, S Ozono, T Yoshie, K Kubota, Y Hirao, N Konishi, H Hashimoto, M Ohshima, Y Hiasa, E Okajima","doi":"","DOIUrl":"","url":null,"abstract":"We have generated a monoclonal antibody against prostatic adenocarcinoma, PC-Ab (IgM) was derived from a fusion using the fresh prostatic tissue of adenocarcinoma as the immunogen. Initial screening was performed by enzyme-linked immunosorbent assay (ELISA) on the soluble fraction of the immunogen. The specific analysis was performed by the avidin-biotin-complex immunoperoxidase method on paraffin-embedded sections of normal, benign and malignant neoplastic tissues from the prostate and various organs. PC-Ab reacted with well differentiated adenocarcinoma (83.3%), moderately differentiated adenocarcinoma (92.2%), and poorly differentiated adenocarcinoma (90.2%), respectively. According to classification by stage, the reaction rates with stage T0, T1, T2, T3 and T4 were 77.3%, 80.0%, 95.2%, 91.3% and 92.9%, respectively but no significant differences in the stages were seen among these groups. PC-Ab reacted with the epithelium of the normal prostate and benign prostatic adenoma, and human fetal tissues. Molecular weight and isoelectric point of the antigen recognized by this PC-Ab was estimated to be 57,000 daltons and 7.0, respectively. These results indicate that PC-Ab reacts with the antigen associated with human prostatic adenocarcinoma.","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12870662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Common and subtypic determinants of hepatitis B surface antigen particles: susceptibility to reduction and/or alkylation evaluated with monoclonal antibodies. 乙型肝炎表面抗原颗粒的常见和亚型决定因素:单克隆抗体评估对还原和/或烷基化的敏感性

The Japanese journal of experimental medicine Pub Date : 1990-10-01

P Luengrojanakul, H Ohnuma, K Tachibana, S Usuda, H Okamoto, T Tanaka, F Tsuda, A Machida, M Mayumi

{"title":"Common and subtypic determinants of hepatitis B surface antigen particles: susceptibility to reduction and/or alkylation evaluated with monoclonal antibodies.","authors":"P Luengrojanakul, H Ohnuma, K Tachibana, S Usuda, H Okamoto, T Tanaka, F Tsuda, A Machida, M Mayumi","doi":"","DOIUrl":"","url":null,"abstract":"The specificity of five monoclonal antibodies, three raised against hepatitis B surface antigen (HBsAg) particles and two against envelope polypeptides, was tested for on a panel of 366 sera containing HBsAg of various subtypes (131 adw, 146 adr, 39 ayw and 50 ayr). Three monoclonals bound to HBsAg irrespective of subtypes, and therefore, were directed to the common antigenic determinants of HBsAg. Of these, two raised against particles (No. 824 and No. 7922) did not bind with reduced HBsAg particles. The other raised against peptides (No. 5124) bound to reduced HBsAg particles. It did not, however, bind to reduced and alkylated HBsAg particles, thereby indicating that it was directed to an epitope involving cysteine residues not contributing to the conformation. The remaining two monoclonals were directed to subtypic determinants not identical to any of d, y, w and r determinants. The subtypic determinant detectable by one of them (No. 4403), raised against HBsAg polypeptides, markedly increased after reduction of HBsAg particles with or without alkylation. In contrast, the subtypic determinant, detectable by the other monoclonal (No. 2155) raised against particles, substantially decreased after reduction. Non-identity of common or subtypic determinants detectable by the five monoclonals were established by blocking tests in which labeled antibody was competed by non-labeled antibody, of a homologous or heterologous specificity, for the binding with HBsAg. These monoclonals would be useful in studies for immunochemical configuration of HBsAg particles and epidemiology of novel subtypic determinants.","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12870661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The pathological differences of rejection in the heart allografts which are transplanted alone or with synchronous skin grafts in rats. 大鼠同种异体心脏单独移植与同步皮肤移植排斥反应的病理差异。

The Japanese journal of experimental medicine Pub Date : 1990-10-01

K Kogure, S M Lim

引用次数: 0

Renal brushborder membrane vesicle. Study of marker enzymes and uptake of nutrients in Mycobacterium leprae infected mice. 肾刷缘膜小泡。麻风分枝杆菌感染小鼠标记酶及营养物质摄取的研究。

The Japanese journal of experimental medicine Pub Date : 1990-10-01

M Kohli, V K Sharma, C Vaishnavi, N K Ganguly, S Kaur, K S Chugh

引用次数: 0

Strain difference in neoplastic response to DMBA powder dusted onto mammary tissues between Wistar/Furth and Copenhagen strains of rats. Wistar/Furth和Copenhagen大鼠对乳腺组织喷撒DMBA粉末肿瘤反应的品系差异。

The Japanese journal of experimental medicine Pub Date : 1990-10-01

N Kusunose, T Shoji, A Tsubura, M Yamamoto, S Morii

{"title":"Strain difference in neoplastic response to DMBA powder dusted onto mammary tissues between Wistar/Furth and Copenhagen strains of rats.","authors":"N Kusunose, T Shoji, A Tsubura, M Yamamoto, S Morii","doi":"","DOIUrl":"","url":null,"abstract":"To investigate genetic and sex factors in the local tumorigenesis by 7,12-dimethylbenz(alpha)anthracene (DMBA) in the mammary gland, both males and females of two different inbred strains of rats, Wistar/Furth (WF) and Copenhagen (COP), were subjected to dusting with approximately 1 mg of DMBA powder directly onto the exposed inguinal mammary tissue at 30 days of age. Locally growing tumors to 2 cm in mean diameter were harvested during 28 weeks after the carcinogen application. The incidence of macroscopic tumors of mammary origin was 100% in 15 WF females, 19% in 16 WF males, 85% in 20 COP females, and 74% in 19 COP males. Histologic pictures indicated the carcinomatous pattern composing mainly of differentiated adenocarcinoma of ductular cells in 12 tumors (80%) from WF females but not in any tumors from the other groups. On the other hand, they showed the sarcomatous pattern characterized by undifferentiated sarcoma of stromal cells in 2 tumors (13%) from WF females, 3 tumors (19%) from WF males, 16 tumors (80%) from COP females, and 14 tumors (74%) from COP males. The other 2 tumors from 1 WF and 1 COP females revealed the carcinosarcomatous pattern. Therefore, mammary ductular cells of WF are highly susceptible to DMBA and may be modified by sex factors in their carcinogenesis. Mammary stromal cells of COP are extremely susceptible to DMBA independently of sex factors.","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13281980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of hepatitis C virus RNA by a two-stage polymerase chain reaction with two pairs of primers deduced from the 5'-noncoding region. 用从5'-非编码区推导出的两对引物两阶段聚合酶链反应检测丙型肝炎病毒RNA。

The Japanese journal of experimental medicine Pub Date : 1990-08-01

H Okamoto, S Okada, Y Sugiyama, T Tanaka, Y Sugai, Y Akahane, A Machida, S Mishiro, H Yoshizawa, Y Miyakawa

引用次数: 0

Histopathological and immunopathological evaluation of filarial glomerulonephritis in Dirofilaria immitis infected dogs. 免疫双丝虫感染犬丝状肾小球肾炎的组织病理学和免疫病理学评价。

The Japanese journal of experimental medicine Pub Date : 1990-08-01

K Nakagaki, M Hayasaki, I Ohishi

{"title":"Histopathological and immunopathological evaluation of filarial glomerulonephritis in Dirofilaria immitis infected dogs.","authors":"K Nakagaki, M Hayasaki, I Ohishi","doi":"","DOIUrl":"","url":null,"abstract":"Filarial glomerulonephritis was studied using Dirofilaria immitis infected dogs. Of 34 infected dogs examined, 15 dogs (44.1%) had histopathological lesions in the kidney. These lesions included an increased number of mesangial cells and increased thickness of the matrix, the infiltration of the small round and plasma cells into the interstitium and thickening of the basement membrane. Deposits of IgG were demonstrated in the infected dogs, whereas C3 deposits were found in all dogs. Combined immunoglobulin and complement deposits were not always found in the dogs with histopathological lesions. The mean concentration (expressed as absorbance) of circulating immune complexes (CIC) was 0.675 +/- 0.517 in infected dogs, and 0.132 +/- 0.092 in uninfected dogs. Although there was significant difference in the level of CIC between infected and uninfected dogs (P less than 0.001), 11 dogs (32.4%) in infected group were negative. Otherwise, the CIC levels were correlated to the adult worm burden (r = 0.848; P less than 0.001) but not to the number of circulating microfilariae (mf) (r = 0.398; P less than 0.05). Transfer of mf to 7 naive dogs was performed to clarify the role of mf in the pathogenesis of filariasis. Antibodies to crude mf antigen became detectable two weeks after the transfer. Neither pathologic findings nor deposits of IgG and C3 in the kidney were found in dogs examined 20 days or 70 days after transfer. There was no evidence that histopathological lesions were induced by live mf, suggesting that adult worm burdens may be more closely related to filarial nephropathy.","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13443530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Augmented inhibition of tumor cell proliferation in the combined use of disulfiram with ascorbic acid and the involvement of oxygen free radicals. 双硫仑与抗坏血酸联合使用和氧自由基参与增强肿瘤细胞增殖的抑制作用。

The Japanese journal of experimental medicine Pub Date : 1990-08-01

H Mashiba, K Matsunaga

引用次数: 0

Natural autoantibodies against Tamm-Horsfall glycoprotein in normal individuals in relation to age and in adult patients with kidney diseases. 正常个体与年龄和肾脏疾病成人患者抗Tamm-Horsfall糖蛋白的天然自身抗体

The Japanese journal of experimental medicine Pub Date : 1990-08-01

M Pinto, C Oron, O Pinto, G Peer

引用次数: 0