The Japanese journal of experimental medicine最新文献_第5页

Effects of thermotherapy on the morphology and cell cycle of a transplanted ascites hepatoma in rats. 热疗对大鼠移植腹水肝癌形态及细胞周期的影响。

The Japanese journal of experimental medicine Pub Date : 1990-02-01

H Hiraoka

引用次数: 0

Occurrence and character of a putative causative virus of enterically-transmitted non-A, non-B hepatitis in bile. 胆汁中肠传非甲、非乙型肝炎推定病原病毒的发生和特征。

The Japanese journal of experimental medicine Pub Date : 1990-02-01

T Uchida, K Suzuki, K Komatsu, F Iida, T Shikata, T Rikihisa, K Mizuno, S Soe, K M Win, K Nakane

{"title":"Occurrence and character of a putative causative virus of enterically-transmitted non-A, non-B hepatitis in bile.","authors":"T Uchida, K Suzuki, K Komatsu, F Iida, T Shikata, T Rikihisa, K Mizuno, S Soe, K M Win, K Nakane","doi":"","DOIUrl":"","url":null,"abstract":"The present investigation confirms the possibility that the etiological agent of enterically-transmitted non-A, non-B (ET-NANB) hepatitis (type E hepatitis), multiplied in hepatocytes, is excreted into the feces via bile. The fecal extract was inoculated into 7 cynomolgus monkeys. Bile juice was collected directly from the gallbladder by needle puncture after abdominal operation 3 to 6 times during the experimental course. All 7 monkeys developed elevated serum aminotransferases, which began gradually approximately 2 weeks postinoculation and reached a peak at 3 to 5 weeks. In parallel with this elevation, both in time and magnitude, necroinflammation was observed in the livers. The virus-like particles (VLPs) were found in the bile juice of all 7 monkeys and the serial occurrence of VLPs was typified as follows: the VLPs were negative on day 7, appeared on day 10 after inoculation, and were present until the 3rd week when the subjects were sacrificed. While the particles were individually dispersed on day 10, they started to exhibit spontaneous aggregation on and after week 2. Also, empty particles were very rare at first, but increased in ratio compared to full ones over time. Thus, the putative causative virus of ET-NANB hepatitis was demonstrated to be excreted through bile. The spontaneous aggregation of VLPs might be due to the specific antibody secreted into the bile juice and was closely correlated with hepatitis activity. The increase in empty particles might indicate an increase in disorganized assembly of the nucleic acid and protein during virus proliferation.","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13267819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanism of suppression of footpad reaction in mice by 12-tetradecanoylphorbol 13-acetate and 7,12-dimethylbenz[a]anthracene. 12-十四烷酚13-乙酸酯和7,12-二甲基苯[a]蒽抑制小鼠脚垫反应的机制。

The Japanese journal of experimental medicine Pub Date : 1990-02-01

M Tabara, M Watanabe

{"title":"Mechanism of suppression of footpad reaction in mice by 12-tetradecanoylphorbol 13-acetate and 7,12-dimethylbenz[a]anthracene.","authors":"M Tabara, M Watanabe","doi":"","DOIUrl":"","url":null,"abstract":"We reported previously that footpad reaction (FPR) of BALB/c mice against sheep red blood cells (SRBC) was suppressed by the percutaneal administration of 12-tetradecanoyl 13-acetate (TPA), a tumor promoter, following that of 7,12-dimethylbenz[a]anthracene (DMBA), a tumor initiator. This effect could be transferred with Thy-1(+) and Lyt-2(+) spleen cells. These findings suggested that this effect was caused by induction of the antigen nonspecific T-suppressor cells and that the process of the induction consisted of two steps. In the present report, we studied the cells related to the first step of this process. The spleen cells from the donor mice on which 400 nmol of DMBA was painted were transferred to the recipient mice which had been immunized 1 hr before the transfer. Then, 8 nmol of TPA was painted on the recipient mice every day for a week. Nine days after the transfer, FR against SRBC was measured. FPR in the recipient mice was suppressed compared to the control, showing that the effect of DMBA can be transferred with the spleen cells. The results of the treatment of the cells to be transferred with monoclonal antibodies and complement show that the cells which is responsible for the transfer of the effect are Mac-1(+), Thy-1(-), Lyt-2(-) and Ia(-). Also the effect of DMBA could be transferred with peritoneal macrophages from the donor mice on which DMBA was painted and anti Ia antibody treatment of the macrophages did not abrogate the effect of transfer.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13296132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Schistosoma japonicum: the effect of serum fractions precipitated by ammonium sulfate on viability of schistosomula. 日本血吸虫:硫酸铵沉淀血清组分对血吸虫活力的影响。

The Japanese journal of experimental medicine Pub Date : 1989-12-01

Y Irie, K Yasuraoka

引用次数: 0

Expression of Forssman glycolipid and blood group-related antigens A, Le(x), and Le(y) in human gastric cancer and in fetal tissues. Forssman糖脂和血型相关抗原A、Le(x)和Le(y)在人胃癌和胎儿组织中的表达

The Japanese journal of experimental medicine Pub Date : 1989-12-01

K Uemura, H Hattori, K Ono, H Ogata, T Taketomi

{"title":"Expression of Forssman glycolipid and blood group-related antigens A, Le(x), and Le(y) in human gastric cancer and in fetal tissues.","authors":"K Uemura, H Hattori, K Ono, H Ogata, T Taketomi","doi":"","DOIUrl":"","url":null,"abstract":"The expression of Forssman glycolipid antigen in human gastric cancers was investigated by thin-layer chromatogram immunostaining of glycolipid fractions. Incompatible blood group A antigen, Le(x) and Le(y) antigen were also studied in comparison with Forssman antigen. Forssman glycolipid as the pentaglycosylceramide was demonstrated in nine out of 12 gastric cancers, and in three out of 10 adjacent uninvolved tissues. In most cases the content of Forssman glycolipid was increased in the cancers compared with that in the uninvolved counterparts. Forssman pentaglycosylceramide was also detected in some normal gastric mucosae (two out of four), and in a fetal gastrointestinal tract tissue. In immunohistochemical examination of gastric cancer tissues, sialidase treatment revealed a positive staining with anti-Forssman antibody. Some cancer tissues from patients with blood group O were found to contain blood group A-active glycolipids, which could be distinguished from Forssman glycolipid by thin-layer chromatogram immunostaining. The incidence of incompatible A-active glycolipids was two out of 10 cancers from patients with blood group O or B. Le(x)- and Le(y)-active glycolipids were detected in most of the preparations and were not accumulated consistently in the cancers.","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13836183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of a novel antigen (1G12) on the surface of peripheral blood mononuclear cells from cancer patients by a sensitive radioimmunoassay. A possible marker for the early diagnosis of cancer. 用灵敏的放射免疫分析法检测肿瘤患者外周血单核细胞表面的新抗原(1G12)。这可能是癌症早期诊断的标志。

The Japanese journal of experimental medicine Pub Date : 1989-12-01

H Harada, N Takanashi, Y Tsukada, M Takahashi, E Kimura, Y Terashima, H Nakamura, E Takahashi, Y Hayata

{"title":"Detection of a novel antigen (1G12) on the surface of peripheral blood mononuclear cells from cancer patients by a sensitive radioimmunoassay. A possible marker for the early diagnosis of cancer.","authors":"H Harada, N Takanashi, Y Tsukada, M Takahashi, E Kimura, Y Terashima, H Nakamura, E Takahashi, Y Hayata","doi":"","DOIUrl":"","url":null,"abstract":"A murine monoclonal antibody designated 1G12 has been produced by immunizing mice with A549 human lung adenocarcinoma. Using radiolabeled monoclonal antibody 1G12, a sensitive radioimmunoassay to detect 1G12 antigen has been developed. The antigen was detected on the surface of peripheral blood mononuclear cells (PBMC). A study was performed to examine the frequency of distribution of this antigen on the surface of PBMC from healthy donors and patients with cancer. An antigen level of above 30 units per 1 x 10(6) PBMC was considered positive. None of 41 healthy donors (0%) and 16 of 96 patients (16.7%) with benign diseases of the lung, ovary, and uterus were positive. In contrast, 27 of 41 patients (65.8%) with lung cancer, 14 of 18 patients (77.8%) with ovarian cancer, and 16 of 27 patients (59.2%) with uterine cervical cancer had elevated levels of 1G12 antigen. When patients were grouped by stages of cancer, PBMC from patients in relatively early stages (stages I and II) also gave positive results, i.e., 9 of 17 patients (59.2%) with lung cancer, 6 of 10 patients (60%) with ovarian cancer, and 11 of 22 patients (50%) with uterine cervical cancer of these stages were positive. These results suggest that the detection of 1G12 antigen on PBMC of patients may be useful for early diagnosis of cancers.","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13765290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution of varicella-zoster virus strains carrying a PstI-site-less mutation in Japan and DNA change responsible for the mutation. 携带psti -less位点突变的水痘-带状疱疹病毒株在日本的分布以及导致该突变的DNA变化。

The Japanese journal of experimental medicine Pub Date : 1989-12-01

R Hondo, Y Yogo, M Yoshida, A Fujima, S Itoh

引用次数: 0

Effect of long-term treatment with sulfatide on hyperlipidemia and progression of atherosclerosis in WHHL rabbits. 磺胺脂长期治疗对WHHL家兔高脂血症及动脉粥样硬化进展的影响。

The Japanese journal of experimental medicine Pub Date : 1989-12-01

T Taketomi, A Hara, K Uemura, N Kyogashima

{"title":"Effect of long-term treatment with sulfatide on hyperlipidemia and progression of atherosclerosis in WHHL rabbits.","authors":"T Taketomi, A Hara, K Uemura, N Kyogashima","doi":"","DOIUrl":"","url":null,"abstract":"Changes in serum lipids and lipoproteins and progression of atherosclerosis with age were examined in Watanabe heritable hyperlipidemic (WHHL) rabbits with or without treatment with sulfatide. The injection of sulfatide solution caused a reduction of serum triacylglycerols for five months including the period of treatment, but afterwards, failed to lower the level of total cholesterol, triacylglycerols and phospholipids, and to suppress the progression of atherosclerosis. Concentrations of LDL as a major serum lipoprotein in WHHL rabbits with or without the treatment with sulfatide were found to be about 64-fold those of normal rabbits by immunological assay using anti-LDL antiserum, whereas the contents of total cholesterol, triacylglycerols, and phospholipids in WHHL rabbit sera were found to be about 10-fold those of normal rabbits. All WHHL rabbits with or without the treatment with sulfatide contained very small amounts of HDL. Types of apoproteins of isolated LDL and VLDL fractions suggested that the former seemed to be derived from VLDL remnant, and the latter to be derived from chylomicron remnant. It was noted that all WHHL rabbit sera had a significant increased amount of lysophosphatidylcholine, and that the fatty acid composition of total serum lipids had almost no change except for slight decrease in arachidonic acid with age. Pathological examination showed that severe atherosclerotic lesions were not so different between WHHL rabbits with or without treatment with sulfatide at age over 22 months.","PeriodicalId":22530,"journal":{"name":"The Japanese journal of experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13765291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative and qualitative differences in serum HBV DNA between HBeAg positive carriers and those positive for anti-HBe. HBeAg阳性携带者和抗hbe阳性携带者血清HBV DNA的定量和定性差异。

The Japanese journal of experimental medicine Pub Date : 1989-12-01

H Okamoto, S Yotsumoto, F Tsuda, A Machida, M Mayumi

引用次数: 0

Characterization of carbonic anhydrase-rich cells in the outer medullary collecting duct of rat kidney. 大鼠肾外髓集管富碳酸酐酶细胞的表征。

The Japanese journal of experimental medicine Pub Date : 1989-10-01

K Nitta, W Yumura, K Nakamura, S Horita, H Kogo, T Sanaka, H Nihei, N Sugino

引用次数: 0