TECHNOLOGY最新文献_第3页

Collagen and heparan sulfate coatings differentially alter cell proliferation and attachment in vitro and in vivo. 胶原蛋白和硫酸肝素涂层在体外和体内不同程度地改变细胞增殖和附着。

TECHNOLOGY Pub Date : 2016-09-01 Epub Date: 2016-01-07 DOI: 10.1142/S2339547816400033

Christopher M Walthers, Chase J Lyall, Alireza K Nazemi, Puneet V Rana, James C Y Dunn

引用次数: 1

Electropermanent magnet actuation for droplet ferromicrofluidics. 液滴铁磁微流体的电永磁驱动。

TECHNOLOGY Pub Date : 2016-06-01 Epub Date: 2016-05-13 DOI: 10.1142/S2339547816500023

José I Padovani, Stefanie S Jeffrey, Roger T Howe

引用次数: 13

Dynamic deformability of sickle red blood cells in microphysiological flow. 微生理流动中镰状红细胞的动态变形能力。

TECHNOLOGY Pub Date : 2016-06-01 DOI: 10.1142/S2339547816400045

Y Alapan, Y Matsuyama, J A Little, U A Gurkan

{"title":"Dynamic deformability of sickle red blood cells in microphysiological flow.","authors":"Y Alapan, Y Matsuyama, J A Little, U A Gurkan","doi":"10.1142/S2339547816400045","DOIUrl":"https://doi.org/10.1142/S2339547816400045","url":null,"abstract":"In sickle cell disease (SCD), hemoglobin molecules polymerize intracellularly and lead to a cascade of events resulting in decreased deformability and increased adhesion of red blood cells (RBCs). Decreased deformability and increased adhesion of sickle RBCs lead to blood vessel occlusion (vaso-occlusion) in SCD patients. Here, we present a microfluidic approach integrated with a cell dimensioning algorithm to analyze dynamic deformability of adhered RBC at the single-cell level in controlled microphysiological flow. We measured and compared dynamic deformability and adhesion of healthy hemoglobin A (HbA) and homozygous sickle hemoglobin (HbS) containing RBCs in blood samples obtained from 24 subjects. We introduce a new parameter to assess deformability of RBCs: the dynamic deformability index (DDI), which is defined as the time-dependent change of the cell's aspect ratio in response to fluid flow shear stress. Our results show that DDI of HbS-containing RBCs were significantly lower compared to that of HbA-containing RBCs. Moreover, we observed subpopulations of HbS containing RBCs in terms of their dynamic deformability characteristics: deformable and non-deformable RBCs. Then, we tested blood samples from SCD patients and analyzed RBC adhesion and deformability at physiological and above physiological flow shear stresses. We observed significantly greater number of adhered non-deformable sickle RBCs than deformable sickle RBCs at flow shear stresses well above the physiological range, suggesting an interplay between dynamic deformability and increased adhesion of RBCs in vaso-occlusive events.","PeriodicalId":22332,"journal":{"name":"TECHNOLOGY","volume":"4 2","pages":"71-79"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1142/S2339547816400045","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9395862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 54

High-throughput evaluation of synthetic metabolic pathways. 合成代谢途径的高通量评价。

TECHNOLOGY Pub Date : 2016-03-01 Epub Date: 2015-12-16 DOI: 10.1142/S233954781640001X

Justin R Klesmith, Timothy A Whitehead

引用次数: 2

Microwell arrays reveal cellular heterogeneity during the clonal expansion of transformed human cells. 微孔阵列揭示了转化人细胞克隆扩增过程中的细胞异质性。

TECHNOLOGY Pub Date : 2015-12-01 DOI: 10.1142/S2339547815200046

Tim C Chang, Weiliang Tang, William Jen Hoe Koh, Alexander J E Rettie, Mary J Emond, Raymond J Monnat, Albert Folch

引用次数: 6

Fast sorting of CD4+ T cells from whole blood using glass microbubbles. 利用玻璃微泡快速分选全血中的CD4+ T细胞。

TECHNOLOGY Pub Date : 2015-03-01 DOI: 10.1142/S2339547815500016

Chia-Hsien Hsu, Chihchen Chen, Daniel Irimia, Mehmet Toner

引用次数: 12

"Universal" vitrification of cells by ultra-fast cooling. 超高速冷却“通用”玻璃化细胞。

TECHNOLOGY Pub Date : 2015-03-01 DOI: 10.1142/S2339547815500053

Yun Seok Heo, Sunitha Nagrath, Alessandra L Moore, Mahnaz Zeinali, Daniel Irimia, Shannon L Stott, Thomas L Toth, Mehmet Toner

{"title":"\"Universal\" vitrification of cells by ultra-fast cooling.","authors":"Yun Seok Heo, Sunitha Nagrath, Alessandra L Moore, Mahnaz Zeinali, Daniel Irimia, Shannon L Stott, Thomas L Toth, Mehmet Toner","doi":"10.1142/S2339547815500053","DOIUrl":"https://doi.org/10.1142/S2339547815500053","url":null,"abstract":"Long-term preservation of live cells is critical for a broad range of clinical and research applications. With the increasing diversity of cells that need to be preserved (e.g. oocytes, stem and other primary cells, genetically modified cells), careful optimization of preservation protocols becomes tedious and poses significant limitations for all but the most expert users. To address the challenge of long-term storage of critical, heterogeneous cell types, we propose a universal protocol for cell vitrification that is independent of cell phenotype and uses only low concentrations of cryoprotectant (1.5 M PROH and 0.5 M trehalose). We employed industrial grade microcapillaries made of highly conductive fused silica, which are commonly used for analytical chemistry applications. The minimal mass and thermal inertia of the microcapillaries enabled us to achieve ultrafast cooling rates up to 4,000 K/s. Using the same low, non-toxic concentration of cryoprotectant, we demonstrate high recovery and viability rates after vitrification for human mammary epithelial cells, rat hepatocytes, tumor cells from pleural effusions, and multiple cancer cell lines.","PeriodicalId":22332,"journal":{"name":"TECHNOLOGY","volume":"3 1","pages":"64-71"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1142/S2339547815500053","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33253664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Stain-less staining for computed histopathology. 计算机组织病理学无染色。

TECHNOLOGY Pub Date : 2015-03-01 DOI: 10.1142/S2339547815200010

David Mayerich, Michael J Walsh, Andre Kadjacsy-Balla, Partha S Ray, Stephen M Hewitt, Rohit Bhargava

引用次数: 52

Microengineered cell and tissue systems for drug screening and toxicology applications: Evolution of in-vitro liver technologies. 用于药物筛选和毒理学应用的微工程细胞和组织系统：体外肝脏技术的发展。

TECHNOLOGY Pub Date : 2015-03-01 DOI: 10.1142/S2339547815300012

O B Usta, W J McCarty, S Bale, M Hegde, R Jindal, A Bhushan, I Golberg, M L Yarmush

{"title":"Microengineered cell and tissue systems for drug screening and toxicology applications: Evolution of in-vitro liver technologies.","authors":"O B Usta, W J McCarty, S Bale, M Hegde, R Jindal, A Bhushan, I Golberg, M L Yarmush","doi":"10.1142/S2339547815300012","DOIUrl":"10.1142/S2339547815300012","url":null,"abstract":"The liver performs many key functions, the most prominent of which is serving as the metabolic hub of the body. For this reason, the liver is the focal point of many investigations aimed at understanding an organism's toxicological response to endogenous and exogenous challenges. Because so many drug failures have involved direct liver toxicity or other organ toxicity from liver generated metabolites, the pharmaceutical industry has constantly sought superior, predictive in-vitro models that can more quickly and efficiently identify problematic drug candidates before they incur major development costs, and certainly before they are released to the public. In this broad review, we present a survey and critical comparison of in-vitro liver technologies along a broad spectrum, but focus on the current renewed push to develop \"organs-on-a-chip\". One prominent set of conclusions from this review is that while a large body of recent work has steered the field towards an ever more comprehensive understanding of what is needed, the field remains in great need of several key advances, including establishment of standard characterization methods, enhanced technologies that mimic the in-vivo cellular environment, and better computational approaches to bridge the gap between the in-vitro and in-vivo results.","PeriodicalId":22332,"journal":{"name":"TECHNOLOGY","volume":"3 1","pages":"1-26"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4494128/pdf/nihms691093.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33898374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stochastic variations of migration speed between cells in clonal populations. 克隆群体中细胞间迁移速度的随机变化。

TECHNOLOGY Pub Date : 2014-09-01 DOI: 10.1142/S2339547814200027

Jun Yan, Daniel Irimia

引用次数: 0