TECHNOLOGY最新文献_第4页

Accounting for biological aggregation in heating and imaging of magnetic nanoparticles. 在磁性纳米粒子的加热和成像中考虑生物聚集。

TECHNOLOGY Pub Date : 2014-09-01 DOI: 10.1142/S2339547814500198

Michael L Etheridge, Katie R Hurley, Jinjin Zhang, Seongho Jeon, Hattie L Ring, Christopher Hogan, Christy L Haynes, Michael Garwood, John C Bischof

{"title":"Accounting for biological aggregation in heating and imaging of magnetic nanoparticles.","authors":"Michael L Etheridge, Katie R Hurley, Jinjin Zhang, Seongho Jeon, Hattie L Ring, Christopher Hogan, Christy L Haynes, Michael Garwood, John C Bischof","doi":"10.1142/S2339547814500198","DOIUrl":"10.1142/S2339547814500198","url":null,"abstract":"Aggregation is a known consequence of nanoparticle use in biology and medicine; however, nanoparticle characterization is typically performed under the pretext of well-dispersed, aqueous conditions. Here, we systematically characterize the effects of aggregation on the alternating magnetic field induced heating and magnetic resonance (MR) imaging performance of iron oxide nanoparticles (IONPs) in non-ideal biological systems. Specifically, the behavior of IONP aggregates composed of ~10 nm primary particles, but with aggregate hydrodynamic sizes ranging from 50 nm to 700 nm, was characterized in phosphate buffered saline and fetal bovine serum suspensions, as well as in gels and cells. We demonstrate up to a 50% reduction in heating, linked to the extent of aggregation. To quantify aggregate morphology, we used a combination of hydrodynamic radii distribution, intrinsic viscosity, and electron microscopy measurements to describe the aggregates as quasifractal entities with fractal dimensions in the 1.8-2.0 range. Importantly, we are able to correlate the observed decrease in magnetic field induced heating with a corresponding decrease in longitudinal relaxation rate (R1) in MR imaging, irrespective of the extent of aggregation. Finally, we show in vivo proof-of-principle use of this powerful new imaging method, providing a critical tool for predicting heating in clinical cancer hyperthermia.","PeriodicalId":22332,"journal":{"name":"TECHNOLOGY","volume":"2 3","pages":"214-228"},"PeriodicalIF":0.0,"publicationDate":"2014-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4219565/pdf/nihms-630195.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32799546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of mind-body awareness training on the early learning of a brain-computer interface. 身心意识训练对脑机接口早期学习的影响。

TECHNOLOGY Pub Date : 2014-09-01 DOI: 10.1142/S233954781450023X

Kaitlin Cassady, Albert You, Alex Doud, Bin He

引用次数: 43

Brain-on-a-chip microsystem for investigating traumatic brain injury: Axon diameter and mitochondrial membrane changes play a significant role in axonal response to strain injuries. 研究外伤性脑损伤的脑芯片微系统:轴突直径和线粒体膜的变化在轴突应变损伤反应中起重要作用。

TECHNOLOGY Pub Date : 2014-06-01 DOI: 10.1142/S2339547814500095

Jean-Pierre Dollé, Barclay Morrison, Rene S Schloss, Martin L Yarmush

{"title":"Brain-on-a-chip microsystem for investigating traumatic brain injury: Axon diameter and mitochondrial membrane changes play a significant role in axonal response to strain injuries.","authors":"Jean-Pierre Dollé, Barclay Morrison, Rene S Schloss, Martin L Yarmush","doi":"10.1142/S2339547814500095","DOIUrl":"https://doi.org/10.1142/S2339547814500095","url":null,"abstract":"Diffuse axonal injury (DAI) is a devastating consequence of traumatic brain injury, resulting in significant axon and neuronal degeneration. Currently, therapeutic options are limited. Using our brain-on-a-chip device, we evaluated axonal responses to DAI. We observed that axonal diameter plays a significant role in response to strain injury, which correlated to delayed elasticity and inversely correlated to axonal beading and axonal degeneration. When changes in mitochondrial membrane potential (MMP) were monitored an applied strain injury threshold was noted, below which delayed hyperpolarization was observed and above which immediate depolarization occurred. When the NHE-1 inhibitor EIPA was administered before injury, inhibition in both hyperpolarization and depolarization occurred along with axonal degeneration. Therefore, axonal diameter plays a significant role in strain injury and our brain-on-a-chip technology can be used both to understand the biochemical consequences of DAI and screen for potential therapeutic agents.","PeriodicalId":22332,"journal":{"name":"TECHNOLOGY","volume":"2 2","pages":"106"},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1142/S2339547814500095","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32567007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Rapid ratiometric biomarker detection with topically applied SERS nanoparticles. 局部应用SERS纳米颗粒的快速比例生物标志物检测。

TECHNOLOGY Pub Date : 2014-06-01 DOI: 10.1142/S2339547814500125

Yu Winston Wang, Altaz Khan, Madhura Som, Danni Wang, Ye Chen, Steven Y Leigh, Daphne Meza, Patrick Z McVeigh, Brian C Wilson, Jonathan T C Liu

{"title":"Rapid ratiometric biomarker detection with topically applied SERS nanoparticles.","authors":"Yu Winston Wang, Altaz Khan, Madhura Som, Danni Wang, Ye Chen, Steven Y Leigh, Daphne Meza, Patrick Z McVeigh, Brian C Wilson, Jonathan T C Liu","doi":"10.1142/S2339547814500125","DOIUrl":"https://doi.org/10.1142/S2339547814500125","url":null,"abstract":"Multiplexed surface-enhanced Raman scattering (SERS) nanoparticles (NPs) offer the potential for rapid molecular phenotyping of tissues, thereby enabling accurate disease detection as well as patient stratification to guide personalized therapies or to monitor treatment outcomes. The clinical success of molecular diagnostics based on SERS NPs would be facilitated by the ability to accurately identify tissue biomarkers under time-constrained staining and detection conditions with a portable device. In vitro, ex vivo and in vivo experiments were performed to optimize the technology and protocols for the rapid detection (0.1-s integration time) of multiple cell-surface biomarkers with a miniature fiber-optic spectral-detection probe following a brief (5 min) topical application of SERS NPs on tissues. Furthermore, we demonstrate that the simultaneous detection and ratiometric quantification of targeted and nontargeted NPs allows for an unambiguous assessment of molecular expression that is insensitive to nonspecific variations in NP concentrations.","PeriodicalId":22332,"journal":{"name":"TECHNOLOGY","volume":"2 2","pages":"118-132"},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1142/S2339547814500125","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32522380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 62

Eradication of multidrug-resistant A. baumannii in burn wounds by antiseptic pulsed electric field. 抗菌脉冲电场根除烧伤创面耐多药鲍曼不动杆菌。

TECHNOLOGY Pub Date : 2014-06-01

Alexander Golberg, G Felix Broelsch, Daniela Vecchio, Saiqa Khan, Michael R Hamblin, William G Austen, Robert L Sheridan, Martin L Yarmush

{"title":"Eradication of multidrug-resistant A. baumannii in burn wounds by antiseptic pulsed electric field.","authors":"Alexander Golberg, G Felix Broelsch, Daniela Vecchio, Saiqa Khan, Michael R Hamblin, William G Austen, Robert L Sheridan, Martin L Yarmush","doi":"","DOIUrl":"","url":null,"abstract":"Emerging bacterial resistance to multiple drugs is an increasing problem in burn wound management. New non-pharmacologic interventions are needed for burn wound disinfection. Here we report on a novel physical method for disinfection: antiseptic pulsed electric field (PEF) applied externally to the infected burns. In a mice model, we show that PEF can reduce the load of multidrug resistant Acinetobacter baumannii present in a full thickness burn wound by more than four orders of magnitude, as detected by bioluminescence imaging. Furthermore, using a finite element numerical model, we demonstrate that PEF provides non-thermal, homogeneous, full thickness treatment for the burn wound, thus, overcoming the limitation of treatment depth for many topical antimicrobials. These modeling tools and our in vivo results will be extremely useful for further translation of the PEF technology to the clinical setting, as they provide the essential elements for planning of electrode design and treatment protocol.","PeriodicalId":22332,"journal":{"name":"TECHNOLOGY","volume":"2 2","pages":"153-160"},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4117356/pdf/nihms593617.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32557855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A microfluidic device to select for cells based on chemotactic phenotype. 一种基于趋化表型的细胞选择微流控装置。

TECHNOLOGY Pub Date : 2014-06-01 DOI: 10.1142/S2339547814200015

Saumendra Bajpai, Michael J Mitchell, Michael R King, Cynthia A Reinhart-King

引用次数: 7

Aqueous two-phase systems enable multiplexing of homogeneous immunoassays. 水两相系统使均质免疫测定的多路复用。

TECHNOLOGY Pub Date : 2014-06-01 DOI: 10.1142/S2339547814500150

Arlyne B Simon, John P Frampton, Nien-Tsu Huang, Katsuo Kurabayashi, Sophie Paczesny, Shuichi Takayama

{"title":"Aqueous two-phase systems enable multiplexing of homogeneous immunoassays.","authors":"Arlyne B Simon, John P Frampton, Nien-Tsu Huang, Katsuo Kurabayashi, Sophie Paczesny, Shuichi Takayama","doi":"10.1142/S2339547814500150","DOIUrl":"https://doi.org/10.1142/S2339547814500150","url":null,"abstract":"Quantitative measurement of protein biomarkers is critical for biomarker validation and early disease detection. Current multiplex immunoassays are time consuming costly and can suffer from low accuracy. For example, multiplex ELISAs require multiple, tedious, washing and blocking steps. Moreover, they suffer from nonspecific antibody cross-reactions, leading to high background and false-positive signals. Here, we show that co-localizing antibody-bead pairs in an aqueous two-phase system (ATPS) enables multiplexing of sensitive, no-wash, homogeneous assays, while preventing nonspecific antibody cross-reactions. Our cross-reaction-free, multiplex assay can simultaneously detect picomolar concentrations of four protein biomarkers ((C-X-C motif) ligand 10 (CXCL10), CXCL9, interleukin (IL)-8 and IL-6) in cell supernatants using a single assay well. The potential clinical utility of the assay is demonstrated by detecting diagnostic biomarkers (CXCL10 and CXCL9) in plasma from 88 patients at the onset of the clinical symptoms of chronic graft-versus-host disease (GVHD).","PeriodicalId":22332,"journal":{"name":"TECHNOLOGY","volume":"2 2","pages":"176"},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1142/S2339547814500150","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32552885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Faraday instability-based micro droplet ejection for inhalation drug delivery. 基于法拉第不稳定性的微滴喷射吸入给药。

TECHNOLOGY Pub Date : 2014-03-01 DOI: 10.1142/S233954781450006X

C S Tsai, R W Mao, S K Lin, Y Zhu, S C Tsai

引用次数: 16

Diffeomorphometry and geodesic positioning systems for human anatomy. 人体解剖用差形测量和测地线定位系统。

TECHNOLOGY Pub Date : 2014-03-01 DOI: 10.1142/S2339547814500010

Michael I Miller, Laurent Younes, Alain Trouvé

{"title":"Diffeomorphometry and geodesic positioning systems for human anatomy.","authors":"Michael I Miller, Laurent Younes, Alain Trouvé","doi":"10.1142/S2339547814500010","DOIUrl":"https://doi.org/10.1142/S2339547814500010","url":null,"abstract":"The Computational Anatomy project has largely been a study of large deformations within a Riemannian framework as an efficient point of view for generating metrics between anatomical configurations. This approach turns D'Arcy Thompson's comparative morphology of human biological shape and form into a metrizable space. Since the metric is constructed based on the geodesic length of the flows of diffeomorphisms connecting the forms, we call it diffeomorphometry. Just as importantly, since the flows describe algebraic group action on anatomical submanifolds and associated functional measurements, they become the basis for positioning information, which we term geodesic positioning. As well the geodesic connections provide Riemannian coordinates for locating forms in the anatomical orbit, which we call geodesic coordinates. These three components taken together - the metric, geodesic positioning of information, and geodesic coordinates - we term the geodesic positioning system. We illustrate via several examples in human and biological coordinate systems and machine learning of the statistical representation of shape and form.","PeriodicalId":22332,"journal":{"name":"TECHNOLOGY","volume":"2 1","pages":"36"},"PeriodicalIF":0.0,"publicationDate":"2014-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1142/S2339547814500010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32402322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 61

Organomatics and organometrics: Novel platforms for long-term whole-organ culture. 器官学和器官计量学:长期全器官培养的新平台。

TECHNOLOGY Pub Date : 2014-03-01 DOI: 10.1142/S2339547814300029

Bote G Bruinsma, Martin L Yarmush, Korkut Uygun

引用次数: 12