TECHNOLOGY最新文献_第2页

Multi-omic network-based interrogation of rat liver metabolism following gastric bypass surgery featuring SWATH proteomics. 利用 SWATH 蛋白组学对胃旁路手术后大鼠肝脏新陈代谢进行基于多组学网络的分析。

TECHNOLOGY Pub Date : 2017-09-01 Epub Date: 2017-09-26 DOI: 10.1142/S233954781750008X

Gautham Vivek Sridharan, Matthew D'Alessandro, Shyam Sundhar Bale, Vicky Bhagat, Hugo Gagnon, John M Asara, Korkut Uygun, Martin L Yarmush, Nima Saeidi

{"title":"Multi-omic network-based interrogation of rat liver metabolism following gastric bypass surgery featuring SWATH proteomics.","authors":"Gautham Vivek Sridharan, Matthew D'Alessandro, Shyam Sundhar Bale, Vicky Bhagat, Hugo Gagnon, John M Asara, Korkut Uygun, Martin L Yarmush, Nima Saeidi","doi":"10.1142/S233954781750008X","DOIUrl":"10.1142/S233954781750008X","url":null,"abstract":"Morbidly obese patients often elect for Roux-en-Y gastric bypass (RYGB), a form of bariatric surgery that triggers a remarkable 30% reduction in excess body weight and reversal of insulin resistance for those who are type II diabetic. A more complete understanding of the underlying molecular mechanisms that drive the complex metabolic reprogramming post-RYGB could lead to innovative non-invasive therapeutics that mimic the beneficial effects of the surgery, namely weight loss, achievement of glycemic control, or reversal of non-alcoholic steatohepatitis (NASH). To facilitate these discoveries, we hereby demonstrate the first multi-omic interrogation of a rodent RYGB model to reveal tissue-specific pathway modules implicated in the control of body weight regulation and energy homeostasis. In this study, we focus on and evaluate liver metabolism three months following RYGB in rats using both SWATH proteomics, a burgeoning label free approach using high resolution mass spectrometry to quantify protein levels in biological samples, as well as MRM metabolomics. The SWATH analysis enabled the quantification of 1378 proteins in liver tissue extracts, of which we report the significant down-regulation of Thrsp and Acot13 in RYGB as putative targets of lipid metabolism for weight loss. Furthermore, we develop a computational graph-based metabolic network module detection algorithm for the discovery of non-canonical pathways, or sub-networks, enriched with significantly elevated or depleted metabolites and proteins in RYGB-treated rat livers. The analysis revealed a network connection between the depleted protein Baat and the depleted metabolite taurine, corroborating the clinical observation that taurine-conjugated bile acid levels are perturbed post-RYGB.","PeriodicalId":22332,"journal":{"name":"TECHNOLOGY","volume":"5 3","pages":"139-184"},"PeriodicalIF":0.0,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5956888/pdf/nihms910203.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36115228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypoxia Impairs Mesenchymal Stromal Cell-Induced Macrophage M1 to M2 Transition. 缺氧损害间充质基质细胞诱导的巨噬细胞M1向M2的转化。

TECHNOLOGY Pub Date : 2017-06-01 DOI: 10.1142/S2339547817500042

Renea A Faulknor, Melissa A Olekson, Emmanuel C Ekwueme, Paulina Krzyszczyk, Joseph W Freeman, François Berthiaume

{"title":"Hypoxia Impairs Mesenchymal Stromal Cell-Induced Macrophage M1 to M2 Transition.","authors":"Renea A Faulknor, Melissa A Olekson, Emmanuel C Ekwueme, Paulina Krzyszczyk, Joseph W Freeman, François Berthiaume","doi":"10.1142/S2339547817500042","DOIUrl":"https://doi.org/10.1142/S2339547817500042","url":null,"abstract":"The transition of macrophages from the pro-inflammatory M1 to the anti-inflammatory M2 phenotype is crucial for the progression of normal wound healing. Persistent M1 macrophages within the injury site may lead to an uncontrolled macrophage-mediated inflammatory response and ultimately a failure of the wound healing cascade, leading to chronic wounds. Mesenchymal stromal cells (MSCs) have been widely reported to promote M1 to M2 macrophage transition; however, it is unclear whether MSCs can drive this transition in the hypoxic environment typically observed in chronic wounds. Here we report on the effect of hypoxia (1% O2) on MSCs' ability to transition macrophages from the M1 to the M2 phenotype. While hypoxia had no effect on MSC secretion, it inhibited MSC-induced M1 to M2 macrophage transition, and suppressed macrophage expression and production of the anti-inflammatory mediator interleukin-10 (IL-10). These results suggest that hypoxic environments may impede the therapeutic effects of MSCs.","PeriodicalId":22332,"journal":{"name":"TECHNOLOGY","volume":"5 2","pages":"81-86"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1142/S2339547817500042","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35923931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

μNeurocircuitry: Establishing in vitro models of neurocircuits with human neurons. μ神经回路:用人神经元建立体外神经回路模型。

TECHNOLOGY Pub Date : 2017-06-01 DOI: 10.1142/S2339547817500054

Joseph A Fantuzzo, Lidia De Filippis, Heather McGowan, Nan Yang, Yi-Han Ng, Apoorva Halikere, Jing-Jing Liu, Ronald P Hart, Marius Wernig, Jefrey D Zahn, Zhiping P Pang

{"title":"μNeurocircuitry: Establishing in vitro models of neurocircuits with human neurons.","authors":"Joseph A Fantuzzo, Lidia De Filippis, Heather McGowan, Nan Yang, Yi-Han Ng, Apoorva Halikere, Jing-Jing Liu, Ronald P Hart, Marius Wernig, Jefrey D Zahn, Zhiping P Pang","doi":"10.1142/S2339547817500054","DOIUrl":"https://doi.org/10.1142/S2339547817500054","url":null,"abstract":"Neurocircuits in the human brain govern complex behavior and involve connections from many different neuronal subtypes from different brain regions. Recent advances in stem cell biology have enabled the derivation of patient-specific human neuronal cells of various subtypes for the study of neuronal function and disease pathology. Nevertheless, one persistent challenge using these human-derived neurons is the ability to reconstruct models of human brain circuitry. To overcome this obstacle, we have developed a compartmentalized microfluidic device, which allows for spatial separation of cell bodies of different human-derived neuronal subtypes (excitatory, inhibitory and dopaminergic) but is permissive to the spreading of projecting processes. Induced neurons (iNs) cultured in the device expressed pan-neuronal markers and subtype specific markers. Morphologically, we demonstrate defined synaptic contacts between selected neuronal subtypes by synapsin staining. Functionally, we show that excitatory neuronal stimulation evoked excitatory postsynaptic current responses in the neurons cultured in a separate chamber.","PeriodicalId":22332,"journal":{"name":"TECHNOLOGY","volume":"5 2","pages":"87-97"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1142/S2339547817500054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35296396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Ultrasound imaging and segmentation of bone surfaces: A review. 骨表面超声成像与分割:综述。

TECHNOLOGY Pub Date : 2017-06-01 Epub Date: 2017-03-31 DOI: 10.1142/S2339547817300049

Ilker Hacihaliloglu

引用次数: 49

Rapid antibiotic sensitivity testing in microwell arrays. 微孔阵列快速抗生素敏感性试验。

TECHNOLOGY Pub Date : 2017-06-01 Epub Date: 2017-05-16 DOI: 10.1142/S2339547817500030

Fatemeh Jalali, Felix Ellett, Daniel Irimia

{"title":"Rapid antibiotic sensitivity testing in microwell arrays.","authors":"Fatemeh Jalali, Felix Ellett, Daniel Irimia","doi":"10.1142/S2339547817500030","DOIUrl":"https://doi.org/10.1142/S2339547817500030","url":null,"abstract":"The widespread bacterial resistance to a broad range of antibiotics necessitates rapid antibiotic susceptibility testing before effective treatment could start in the clinic. Among resistant bacteria, Staphylococcus aureus is one of the most important, and Methicillin-resistant (MRSA) strains are a common cause of life threatening infections. However, standard susceptibility testing for S. aureus is time consuming and thus the start of effective antibiotic treatment is often delayed. To circumvent the limitations of current susceptibility testing systems, we designed an assay that enables measurements of bacterial growth with higher spatial and temporal resolution than standard techniques. The assay consists of arrays of microwells that confine small number of bacteria in small spaces, where their growth is monitored with high precision. These devices enabled us to investigate the effect of different antibiotics on S. aureus growth. We measured the Minimal Inhibitory Concentration (MIC) in less than 3 hours. In addition to being significantly faster than the 48 hours needed for traditional microbiological methods, the assay is also capable of differentiating the specific effects of different antibiotic classes on S. aureus growth. Overall, this assay has the potential to become a rapid, sensitive, and robust tool for use in hospitals and laboratories to assess antibiotic sensitivity.","PeriodicalId":22332,"journal":{"name":"TECHNOLOGY","volume":"5 2","pages":"107-114"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1142/S2339547817500030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35296397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Building an experimental model of the human body with non-physiological parameters. 建立非生理参数的人体实验模型。

TECHNOLOGY Pub Date : 2017-03-01 Epub Date: 2017-03-31 DOI: 10.1142/S2339547817500029

Joseph M Labuz, Christopher Moraes, David R Mertz, Brendan M Leung, Shuichi Takayama

{"title":"Building an experimental model of the human body with non-physiological parameters.","authors":"Joseph M Labuz, Christopher Moraes, David R Mertz, Brendan M Leung, Shuichi Takayama","doi":"10.1142/S2339547817500029","DOIUrl":"https://doi.org/10.1142/S2339547817500029","url":null,"abstract":"New advances in engineering and biomedical technology have enabled recent efforts to capture essential aspects of human physiology in microscale, in-vitro systems. The application of these advances to experimentally model complex processes in an integrated platform - commonly called a 'human-on-a-chip (HOC)' - requires that relevant compartments and parameters be sized correctly relative to each other and to the system as a whole. Empirical observation, theoretical treatments of resource distribution systems and natural experiments can all be used to inform rational design of such a system, but technical and fundamental challenges (e.g. small system blood volumes and context-dependent cell metabolism, respectively) pose substantial, unaddressed obstacles. Here, we put forth two fundamental principles for HOC design: inducing in-vivo-like cellular metabolic rates is necessary and may be accomplished in-vitro by limiting O2 availability and that the effects of increased blood volumes on drug concentration can be mitigated through pharmacokinetics-based treatments of solute distribution. Combining these principles with natural observation and engineering workarounds, we derive a complete set of design criteria for a practically realizable, physiologically faithful, five-organ millionth-scale (× 10-6) microfluidic model of the human body.","PeriodicalId":22332,"journal":{"name":"TECHNOLOGY","volume":"5 1","pages":"42-59"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1142/S2339547817500029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35173704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Cell-free vascular grafts: Recent developments and clinical potential. 无细胞血管移植:最近的发展和临床潜力。

TECHNOLOGY Pub Date : 2017-03-01 Epub Date: 2017-03-31 DOI: 10.1142/S2339547817400015

Sindhu Row, Ana Santandreu, Daniel D Swartz, Stelios T Andreadis

引用次数: 18

Recent advances in nonbiofouling PDMS surface modification strategies applicable to microfluidic technology. 适用于微流控技术的无生物污损 PDMS 表面改性策略的最新进展。

TECHNOLOGY Pub Date : 2017-03-01 Epub Date: 2017-02-07 DOI: 10.1142/S2339547817300013

Aslihan Gokaltun, Martin L Yarmush, Ayse Asatekin, O Berk Usta

{"title":"Recent advances in nonbiofouling PDMS surface modification strategies applicable to microfluidic technology.","authors":"Aslihan Gokaltun, Martin L Yarmush, Ayse Asatekin, O Berk Usta","doi":"10.1142/S2339547817300013","DOIUrl":"10.1142/S2339547817300013","url":null,"abstract":"In the last decade microfabrication processes including rapid prototyping techniques have advanced rapidly and achieved a fairly mature stage. These advances have encouraged and enabled the use of microfluidic devices by a wider range of users with applications in biological separations and cell and organoid cultures. Accordingly, a significant current challenge in the field is controlling biomolecular interactions at interfaces and the development of novel biomaterials to satisfy the unique needs of the biomedical applications. Poly(dimethylsiloxane) (PDMS) is one of the most widely used materials in the fabrication of microfluidic devices. The popularity of this material is the result of its low cost, simple fabrication allowing rapid prototyping, high optical transparency, and gas permeability. However, a major drawback of PDMS is its hydrophobicity and fast hydrophobic recovery after surface hydrophilization. This results in significant nonspecific adsorption of proteins as well as small hydrophobic molecules such as therapeutic drugs limiting the utility of PDMS in biomedical microfluidic circuitry. Accordingly, here, we focus on recent advances in surface molecular treatments to prevent fouling of PDMS surfaces towards improving its utility and expanding its use cases in biomedical applications.","PeriodicalId":22332,"journal":{"name":"TECHNOLOGY","volume":"5 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501164/pdf/nihms867054.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35156741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A cost-effective micromilling platform for rapid prototyping of microdevices. 一个具有成本效益的微铣削平台，用于微器件的快速原型设计。

TECHNOLOGY Pub Date : 2016-12-01 Epub Date: 2016-12-23 DOI: 10.1142/S2339547816200041

Daniel P Yen, Yuta Ando, Keyue Shen

引用次数: 22

Donor variability among anti-inflammatory pre-activated mesenchymal stromal cells. 抗炎前活化间充质间质细胞的供体差异性。

TECHNOLOGY Pub Date : 2016-09-01 DOI: 10.1142/S2339547816500084

Andrea Gray, Rene S Schloss, Martin Yarmush

{"title":"Donor variability among anti-inflammatory pre-activated mesenchymal stromal cells.","authors":"Andrea Gray, Rene S Schloss, Martin Yarmush","doi":"10.1142/S2339547816500084","DOIUrl":"https://doi.org/10.1142/S2339547816500084","url":null,"abstract":"Therapeutic mesenchymal stromal cells (MSCs) are attractive in part due to their immunomodulatory properties, achieved by their paracrine secretion of factors including prostaglandin E2 (PGE2). Despite promising pre-clinical data, demonstrating clinical efficacy has proven difficult. The current studies were designed to develop approaches to pre-induce desired functions from naïve MSCs and examine MSC donor variability, two factors contributing to this disconnect. MSCs from six human donors were pre-activated with interleukin 1 beta (IL-1β) at a concentration and duration identified as optimal or interferon gamma (IFN-γ) as a comparator. Their secretion of PGE2 after pre-activation and secondary exposure to pro-inflammatory molecules was measured. Modulation of tumor necrosis factor alpha (TNF-α) secretion from M1 pro-inflammatory macrophages by co-cultured pre-activated MSCs was also measured. Our results indicated that pre-activation of MSCs with IL-1β resulted in upregulated PGE2 secretion post exposure. Pre-activation with IL-1β or IFN-γ resulted in higher sensitivity to induction by secondary stimuli compared to no pre-activation. While IL-1β pre-activation led to enhanced MSC-mediated attenuation of macrophage TNF-α secretion, IFN-γ pre-activation resulted in enhanced TNF-α secretion. Donor variability was noted in PGE2 secretion and upregulation and the level of improved or impaired macrophage modulation.","PeriodicalId":22332,"journal":{"name":"TECHNOLOGY","volume":"4 3","pages":"201-215"},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1142/S2339547816500084","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36074458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15