Systematic Entomology最新文献

{"title":"Genome size evolution in grasshoppers (Orthoptera: Caelifera: Acrididae)","authors":"Kuo Sun,&nbsp;Yingchun Lu,&nbsp;Yuan Huang,&nbsp;Huateng Huang","doi":"10.1111/syen.12586","DOIUrl":"10.1111/syen.12586","url":null,"abstract":"<p>Grasshoppers (Orthoptera: Acrididae) are known for their significantly enlarged genome compared to other insects. However, our understanding of the evolutionary dynamics of genome size (GS) with this family is still limited. This study measured the GS of 62 grasshopper species using flow cytometry and assembled 10 new mitochondrial genomes for comparative phylogenetic analyses. An expanded species sampling discovered several grasshopper species with giant GS surpassing the previous insect record. We then applied recently developed methods to test more complicated, heterogeneous evolutionary models. We found that grasshopper GS has a strong phylogenetic signal and does not correlate with species' body size or flight ability. These results support the neutral or near-neutral hypotheses of GS evolution. However, GS had accelerated rates of evolution on some grasshopper lineages, suggesting heterogeneity in its evolutionary dynamics. Ancestral state reconstruction indicates that the large genome evolved before the origin of the Acrididae family. Future studies with more species measurements will help assess the frequency of macroevolutionary shifts and identify possible mechanisms for these shifts in grasshopper GS evolution.</p>","PeriodicalId":22126,"journal":{"name":"Systematic Entomology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2023-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45658906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogeny of the Poritiinae (Lepidoptera: Lycaenidae), butterflies with ant associations and unusual lichenivorous diets","authors":"John H. Boyle,&nbsp;Marianne Espeland,&nbsp;Szabolcs Sáfián,&nbsp;Robert Ducarme,&nbsp;Alan J. Gardiner,&nbsp;James W. Coleman,&nbsp;Alan Heath,&nbsp;Stewart Fisher,&nbsp;Steve C. Collins,&nbsp;Dino J. Martins,&nbsp;Kwaku Aduse-Poku,&nbsp;Michel Libert,&nbsp;Even Dankowicz,&nbsp;Akito Y. Kawahara,&nbsp;David J. Lohman,&nbsp;Naomi E. Pierce","doi":"10.1111/syen.12585","DOIUrl":"10.1111/syen.12585","url":null,"abstract":"<p>The Poritiinae are a diverse subfamily of lycaenid butterflies with about 700 species divided into two major groups: the Asian endemic tribe Poritiini, and the African endemic tribe Liptenini. Among these, the Liptenini are notable for their lichenivorous diet and the strong but apparently non-mutualistic ant associations of many species. We present the first molecular phylogeny for this subfamily, based on data from 14 gene regions, and including 218 representatives from 177 taxa (approximately 25% of species) in 50 of the 58 (86%) recognized genera. From this analysis, we confirm the division of the subfamily into two tribes, and we rearrange the Liptenini tribe into six subtribes, Durbaniina, Pentilina, Liptenina, Iridanina and Epitolina, plus a new tribe, Cooksoniina <b>subtrib. n.</b>, to fill a gap in the nomenclature revealed by the phylogenetic analysis. We also point to several genera in need of further taxonomic revision. Ancestral range reconstruction could not infer the range of the common ancestor of the Poritiinae; however, the common ancestor of the Poritiini was likely Asian, while that of the Liptenini was likely African, with subsequent narrowing of ranges in several lineages.</p>","PeriodicalId":22126,"journal":{"name":"Systematic Entomology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/syen.12585","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45296863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Origin, evolution and systematics of the genus Poecilimon (Orthoptera: Tettigoniidae)—An outburst of diversification in the Aegean area","authors":"Simeon B. Borissov,&nbsp;Klaus-Gerhard Heller,&nbsp;Battal Çıplak,&nbsp;Dragan P. Chobanov","doi":"10.1111/syen.12580","DOIUrl":"10.1111/syen.12580","url":null,"abstract":"<p>Our study focuses on the origin, dispersal patterns, evolutionary strategies and systematics of <i>Poecilimon</i>, the largest bush-cricket genus in the Palaearctic with over 150 taxa described. We employ phylogenetic and divergence time estimation analyses based on multilocus sequence data (ND2 + COI + 12 S + 16 S + ITS+28 S), perform ancestral area reconstruction, and track the evolution of behavioural (evolution of sound communication) and morpho-physiological traits (body size and shape, and spermatophore size) in this genus. Based on our results, we propose a revised systematics of <i>Poecilimon</i>, including description of a new species, <i>P. nivalis</i> <b>sp. n.</b>, and hypothesize three stages in the evolution of <i>Poecilimon</i>. (1) In the early evolution of the genus in Tortonian, when open dry habitats appeared in the Eastern Mediterranean, diversification rates were low and speciation was possibly induced by vicariance and habitat fragmentation; physiology and morphology during this period retained their ancestral states but the evolution of main lineages may have been accompanied by behavioural specializations. (2) Climate cooling and aridification during the Messinian induced dispersals and adaptation to new habitats, followed by physiological and behavioural adaptations; major clades formed or started diversifying. (3) Starting at the end of Messinian and continuing through the Plio- and Pleistocene, a few dispersal events from Anatolia to the Balkans took place and climatic oscillations were followed by allo- and parapatric divergence of habitat specialists, while ecological adaptations enhanced song diversity and led to morpho-physiological changes.</p>","PeriodicalId":22126,"journal":{"name":"Systematic Entomology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2023-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49299264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inclusion of rare taxa from Blattidae and Anaplectidae improves phylogenetic resolution in the cockroach superfamily Blattoidea","authors":"Wenbo Deng,&nbsp;Xinxing Luo,&nbsp;Simon Y. W. Ho,&nbsp;Shuran Liao,&nbsp;Zongqing Wang,&nbsp;Yanli Che","doi":"10.1111/syen.12560","DOIUrl":"10.1111/syen.12560","url":null,"abstract":"<p>Cockroaches are an ecologically and economically important insect group, but some fundamental aspects of their evolutionary history remain unresolved. In particular, there are outstanding questions about some of the deeper relationships among cockroach families. As a group transferred from Blaberoidea Saussure to Blattoidea Latreille, the evolutionary history of the family Anaplectidae Walker requires re-evaluation. In our study, we infer the phylogeny of Blattoidea based on the mitochondrial genomes of 28 outgroup taxa and 67 ingroup taxa, including 25 newly sequenced blattoid species mainly from the families Anaplectidae and Blattidae Latreille. Our results indicate that Blattoidea is the sister group of the remaining Blattodea Brunner von Wattenwyl and that Blattoidea can be divided into three main clades: Blattidae + Tryonicidae McKittrick &amp; Mackerras, Lamproblattidae McKittrick + Anaplectidae and Termitoidae Latreille + Cryptocercidae Handlirsch. Our analyses provide robust support for previously uncertain hypotheses. The sister group of Termitoidae + Cryptocercidae (Xylophagodea Engel) is inferred to constitute the rest of Blattoidea, for the first time. Within Blattidae, <i>Hebardina</i> Bey-Bienko is placed as the sister lineage to the rest of Blattidae. The subfamily Archiblattinae is polyphyletic, Blattinae is paraphyletic and Polyzosteriinae is monophyletic (Macrocercinae Roth not included); the genus <i>Periplaneta</i> Burmrister is polyphyletic. Based on the results of our phylogenetic analyses, we have revised these taxa. A new subfamily, Hebardininae <b>subfam.nov.</b>, is proposed in Blattidae. Archiblattinae and <i>Shelfordella</i> Adelung are synonymized with Blattinae and <i>Periplaneta</i>, respectively: Archiblattinae Kirby <b>syn.nov.</b> and <i>Shelfordella</i> Adelung <b>syn.nov.</b> Our inferred divergence times indicate that Blattoidea emerged in the Late Triassic, with six families in Blattoidea diverging in the Middle and Late Jurassic. We suggest that the divergences among lineages of Asian Blattidae and Anaplectidae were driven by the uplift of the Himalayas and deglaciation during the Quaternary, leading to the present-day distributions of these taxa.</p>","PeriodicalId":22126,"journal":{"name":"Systematic Entomology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2023-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/syen.12560","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42589056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Image-based taxonomic classification of bulk insect biodiversity samples using deep learning and domain adaptation","authors":"Tomochika Fujisawa,&nbsp;Víctor Noguerales,&nbsp;Emmanouil Meramveliotakis,&nbsp;Anna Papadopoulou,&nbsp;Alfried P. Vogler","doi":"10.1111/syen.12583","DOIUrl":"10.1111/syen.12583","url":null,"abstract":"<p>Complex bulk samples of insects from biodiversity surveys present a challenge for taxonomic identification, which could be overcome by high-throughput imaging combined with machine learning for rapid classification of specimens. These procedures require that taxonomic labels from an existing source data set are used for model training and prediction of an unknown target sample. However, such transfer learning may be problematic for the study of new samples not previously encountered in an image set, for example, from unexplored ecosystems, and require methods of domain adaptation that reduce the differences in the feature distribution of the source and target domains (training and test sets). We assessed the efficiency of domain adaptation for family-level classification of bulk samples of Coleoptera, as a critical first step in the characterization of biodiversity samples. Neural network models trained with images from a global database of Coleoptera were applied to a biodiversity sample from understudied forests in Cyprus as the target. Within-dataset classification accuracy reached 98% and depended on the number and quality of training images, and on dataset complexity. The accuracy of between-datasets predictions (across disparate source–target pairs that do not share any species or genera) was at most 82% and depended greatly on the standardization of the imaging procedure. An algorithm for domain adaptation, domain adversarial training of neural networks (DANN), significantly improved the prediction performance of models trained by non-standardized, low-quality images. Our findings demonstrate that existing databases can be used to train models and successfully classify images from unexplored biota, but the imaging conditions and classification algorithms need careful consideration.</p>","PeriodicalId":22126,"journal":{"name":"Systematic Entomology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/syen.12583","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41419891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inadequate molecular identification protocols for invasive pests threaten biosecurity","authors":"Camiel Doorenweerd,&nbsp;Michael San Jose,&nbsp;Luc Leblanc,&nbsp;Daniel Rubinoff","doi":"10.1111/syen.12581","DOIUrl":"10.1111/syen.12581","url":null,"abstract":"<p>Molecular markers are crucial to identify new pest invasions before they can become established but current methods are often inadequate.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":22126,"journal":{"name":"Systematic Entomology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2022-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49082952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of mitogenomic and morphological data disentangles the systematics of Pollenia and establishes a revised phylogenetic hypothesis for the Polleniidae","authors":"Nikolas P. Johnston,&nbsp;Marcin Piwczyński,&nbsp;Paulina Trzeciak,&nbsp;Kinga Walczak,&nbsp;Krzysztof Szpila","doi":"10.1111/syen.12576","DOIUrl":"10.1111/syen.12576","url":null,"abstract":"<p>The Polleniidae (Diptera) are a family of flies best known for species of the genus <i>Pollenia</i>, which overwinter inside human dwellings. Previously divided across the Calliphoridae, Tachinidae and Rhinophoridae, the polleniid genera have only recently been united. Several studies have utilized molecular data to analyse polleniid phylogenetic relationships, although all have suffered from low taxon sampling or insufficient phylogenetic signal in molecular markers. To alleviate these problems, we utilized two automated organellar genome extraction software, GetOrganelle and MitoFinder, to assemble mitogenomes from genome skimming data from 22 representatives of the polleniid genera: <i>Dexopollenia</i>, <i>Melanodexia</i>, <i>Morinia, Pollenia</i> and <i>Xanthotryxus</i>. From these analyses, we provide 14 new mitogenomes for the Polleniidae and perform phylogenetic analyses of 13 protein-coding mitochondrial genes using both maximum likelihood and Bayesian inference. Subfamilial phylogenetic relationships within the Polleniidae are interrogated and <i>Pollenia</i> is found to form a monophyletic clade sister to <i>Melanodexia, Morinia</i> and <i>Dexopollenia</i>, providing no evidence for the synonymisation of any of these genera. Our topology conflicts with previous morphology-based cladistic interpretations, with the <i>amentaria, griseotomentosa, semicinerea</i> and <i>viatica</i> species-groups resolving as non-monophyletic. We provide support for our topology through analysis of adult morphology and male and female terminalia, while identifying new diagnostic characters for some of the clades of the <i>Pollenia</i>. To test the validity of the current diagnostic morphology in the Polleniidae, newly assembled cytochrome C oxidase subunit 1 (COI) data are combined with a polleniid COI barcode reference library and analysed using the species delimitation software ASAP. COI barcodes support the current morphologically defined species within the <i>Pollenia</i>.</p>","PeriodicalId":22126,"journal":{"name":"Systematic Entomology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2022-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/syen.12576","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42260712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogeny and systematics of Sphaeriusidae (Coleoptera: Myxophaga): minute living fossils with underestimated past and present-day diversity","authors":"Martin Fikáček,&nbsp;Shûhei Yamamoto,&nbsp;Keita Matsumoto,&nbsp;Rolf G. Beutel,&nbsp;David R. Maddison","doi":"10.1111/syen.12571","DOIUrl":"10.1111/syen.12571","url":null,"abstract":"<p>Sphaeriusidae (Coleoptera: Myxophaga) is a group of shiny, blackish and hemispherical riparian beetles, known for their miniaturized bodies. They are worldwide in distribution, but very limited information is available about taxonomic and morphological diversity, and natural and evolutionary history. The aim of this study is to help fill in these gaps. We examined the external morphology of modern representatives using scanning electron microscopy (SEM), and reconstructed the phylogeny of the family using five DNA markers (cytochrome oxidase I, 18S rRNA, 28S rRNA, CAD and wingless). Our results suggest a larger morphological diversity than previously expected, corresponding to the deep genetic divergences of principal lineages. We also examined two inclusions in 99-million-year-old Burmese amber. The integration of all evidence allows us to recognize three genera: the extinct genus †<i>Burmasporum</i> Kirejtshuk, the newly defined genus <i>Bezesporum</i> <b>gen.nov.</b> preserved in Burmese amber (<i>B. burmiticum</i> <b>sp.nov.</b>) and present in the modern fauna of Southeast Asia, and the genus <i>Sphaerius</i> Waltl with a world-wide distribution. <i>Sphaerius</i> species are morphologically highly uniform, with the exception of species from Australia and South Africa, which share some characteristics with <i>Bezesporum</i> <b>gen.nov.</b> despite being resolved as deeply nested lineages of <i>Sphaerius</i> by DNA data. The presence of <i>Bezesporum</i> <b>gen.nov.</b> in Burmese amber and in recent fauna indicates that Sphaeriusidae largely maintained their specific morphology and specialized riparian lifestyle for at least 100 million years. Therefore, they can be considered an exceptionally conserved group, with a minimum of evolutionary changes over a long period. Our study also demonstrates that the species numbers and fine-scale morphological diversity of Sphaeriusidae are larger than expected in both the past and present-day faunas. Both were apparently underestimated due to the minute body size and cryptic habits of these beetles.</p>","PeriodicalId":22126,"journal":{"name":"Systematic Entomology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2022-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45401657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Minute moss beetles in the Southern Hemisphere: Molecular phylogeny, historical biogeography and habitat shifts (Coleoptera: Hydraenidae)","authors":"David T. Bilton,&nbsp;Manfred A. Jäch,&nbsp;Ignacio Ribera,&nbsp;Emmanuel F. A. Toussaint","doi":"10.1111/syen.12567","DOIUrl":"10.1111/syen.12567","url":null,"abstract":"<p>Minute moss beetles (Hydraenidae) are one of the most speciose and widespread families of aquatic Coleoptera, with an estimated 4000 extant species, found in the majority of aquatic habitats from coastal rock pools to mountain streams and from the Arctic Circle to the Antarctic islands. Molecular phylogenetic works have improved our understanding of the evolutionary history of the megadiverse <i>Hydraena</i>, <i>Limnebius</i> and <i>Ochthebius</i> in recent years, but most genera in the family have not yet been included in any phylogenetic analyses, particularly most of those which are restricted to the Southern Hemisphere. Using a multimarker molecular matrix, sampling over 40% of described species richness and 75% of currently recognized genera, we infer a comprehensive molecular phylogeny of these predominantly Gondwanan Hydraenidae. Whilst the genera we focus on are morphologically diverse, and currently classified across all four hydraenid subfamilies, our phylogenetic analyses suggest that these Gondwanan genera may instead constitute a single clade. As a result of our findings, the African genus <i>Oomtelecopon</i> Perkins <b>syn.n.</b> is shown to nest within <i>Coelometopon</i> Janssens, the New Zealand <i>Homalaena</i> Ordish <b>syn.n.</b> and <i>Podaena</i> Ordish <b>syn.n.</b> are synonymised with <i>Orchymontia</i> Broun, and the South African <i>Pterosthetops</i> Perkins <b>syn.n.</b> is synonymised with <i>Prosthetops</i> Waterhouse, resulting in Pterosthetopini Perkins <b>syn.n.</b> being synonymised with Prosthetopini Perkins. <i>Mesoceratops</i> Bilton &amp; Jäch <b>gen.n.</b> is erected to accommodate six former members of <i>Mesoceration</i> Janssens, which is shown to be polyphyletic. We propose the replacement name <i>Orchymontia ordishi</i> Jäch &amp; Bilton <b>nom.n.</b> for <i>Homalaena dilatata</i> Ordish, 1984 (now a junior homonym); altogether 39 new combinations are proposed. Our Bayesian divergence times infer an origin for this ‘Gondwana group’ of genera in Africa plus Madagascar in the mid-Cretaceous and suggest that both vicariant and dispersal processes, together with extinctions, have shaped the biogeographic history of these beetles in the Southern Hemisphere during the Cretaceous, resulting in geographically conserved extant lineages. Finally, we reconstruct ancestral habitat shifts across our phylogeny, revealing numerous changes in habitat occupancy in these genera, including multiple origins of fully terrestrial, humicolous taxa in different regions.</p>","PeriodicalId":22126,"journal":{"name":"Systematic Entomology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2022-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/syen.12567","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43652530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenomic inference of two widespread European leaf miner species complexes suggests mechanisms for sympatric speciation (Lepidoptera: Nepticulidae: Ectoedemia)","authors":"Camiel Doorenweerd,&nbsp;Kyung Min Lee,&nbsp;Erik J. van Nieukerken,&nbsp;Marko Mutanen","doi":"10.1111/syen.12579","DOIUrl":"10.1111/syen.12579","url":null,"abstract":"<p>Leafmining insects have extraordinarily intimate relationships with their host plants and are therefore prime candidates for potential ecological speciation in sympatry, but how commonly this mode of speciation occurs in any group of life remains debated. Prior research on the pygmy moth (Nepticulidae) genus <i>Ectoedemia</i> using morphology, DNA barcoding, and multi-marker phylogenetic approaches left two unresolved species complexes, each with each four described species. In this study, we thoroughly sampled each complex across a range of host plants and localities across Europe. We used a double digest restriction-site associated DNA (ddRAD) sequencing approach to clarify species boundaries. In the <i>E. rubivora</i> complex, ddRAD data resolved all four species, contrary to morphological and COI data, which supports a potential scenario of host plant-driven speciation where the host plant specialization provides an ecological barrier to hybridization. However, we found no indication of host race formation within the oligophagous <i>E. atricollis</i> (Stainton). In the <i>E. subbimaculella</i> complex, SNP data only partly distinguishes between the parapatric <i>E. subbimaculella</i> (Haworth) and <i>E. heringi</i> (Toll), but with some statistical overlap, suggesting incomplete lineage sorting which may represent early phases of host-based ecological speciation, or admixture following a period of isolation.</p>","PeriodicalId":22126,"journal":{"name":"Systematic Entomology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2022-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/syen.12579","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46357206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}