Systematic and applied microbiology最新文献

{"title":"Description of the first cultured representative of “Candidatus Synoicihabitans” genus, isolated from deep-sea sediment of South China Sea","authors":"Tariq Ahmad ,&nbsp;Sidra Erum Ishaq ,&nbsp;Lewen Liang ,&nbsp;Jialin Hou ,&nbsp;Ruize Xie ,&nbsp;Yijing Dong ,&nbsp;Tiantian Yu ,&nbsp;Fengping Wang","doi":"10.1016/j.syapm.2024.126490","DOIUrl":"https://doi.org/10.1016/j.syapm.2024.126490","url":null,"abstract":"<div><p>In this study we describe the first cultured representative of <em>Candidatus</em> Synoicihabitans genus, a novel strain designated as LMO-M01^T, isolated from deep-sea sediment of South China Sea. This bacterium is a facultative aerobe, Gram-negative, non-motile, and has a globular-shaped morphology, with light greenish, small, and circular colonies. Analysis of the 16S rRNA gene sequences of strain LMO-M01^T showed less than 93% similarity to its closest cultured members. Furthermore, employing advanced phylogenomic methods such as comparative genome analysis, average nucleotide identity (ANI), average amino acids identity (AAI), and digital DNA-DNA hybridization (dDDH), placed this novel species within the candidatus genus Synoicihabitans of the family <em>Opitutaceae</em>, Phylum <em>Verrucomicrobiota</em>. The genomic analysis of strain LMO-M01^T revealed 175 genes, encoding putative carbohydrate-active enzymes. This suggests its metabolic potential to degrade and utilize complex polysaccharides, indicating a significant role in carbon cycling and nutrient turnover in deep-sea sediment. In addition, the strain’s physiological capacity to utilize diverse biopolymers such as lignin, xylan, starch, and agar as sole carbon source opens up possibilities for sustainable energy production and environmental remediation. Moreover, the genome sequence of this newly isolated strain has been identified across diverse ecosystems, including marine sediment, fresh water, coral, soil, plants, and activated sludge highlighting its ecological significance and adaptability to various environments. The recovery of strain LMO-M01^T holds promise for taxonomical, ecological and biotechnological applications. Based on the polyphasic data, we propose that this ecologically important strain LMO-M01^T represents a novel genus (previously <em>Candidatus</em>) within the family <em>Opitutaceae</em> of phylum <em>Verrucomicrobiota</em>, for which the name <em>Synoicihabitans lomoniglobus</em> gen. nov., sp. nov. was proposed. The type of strain is LMO-M01^T (= CGMCC 1.61593^T = KCTC 92913^T).</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139700113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiphasic investigations imply transfer of orange-/red-pigmented strains of the bean pathogen Curtobacterium flaccumfaciens pv. flaccumfaciens to a new species as C. aurantiacum sp. nov., elevation of the poinsettia pathogen C. flaccumfaciens pv. poinsettiae to the species level as C. Poinsettiae sp. nov., and synonymy of C. albidum with C. citreum","authors":"Ebrahim Osdaghi ,&nbsp;S. Mohsen Taghavi ,&nbsp;Mozhde Hamidizade ,&nbsp;Mehdi Kariminejhad ,&nbsp;Amal Fazliarab ,&nbsp;Habibeh Hajian Maleki ,&nbsp;Steve Baeyen ,&nbsp;Geraldine Taghouti ,&nbsp;Marie-Agnes Jacques ,&nbsp;Johan Van Vaerenbergh ,&nbsp;Perrine Portier","doi":"10.1016/j.syapm.2024.126489","DOIUrl":"10.1016/j.syapm.2024.126489","url":null,"abstract":"<div><p><em>Curtobacterium flaccumfaciens</em> (<em>Microbacteriaceae</em>), a plant-pathogenic coryneform species includes five pathovars with valid names and a number of proposed – but unvalidated – new members. In this study, phenotypic features and DNA similarity indexes were investigated among all <em>C. flaccumfaciens</em> members. Results showed that the <em>C. flaccumfaciens</em> pv. <em>poinsettiae</em> strains causing bacterial canker of <em>Euphorbia pulcherrima</em> in the USA as well as the orange-/red-pigmented strains of <em>C. flaccumfaciens</em> pv. <em>flaccumfaciens</em> pathogenic on dry beans in Iran are too distinct from each other and from the type strain of the species to be considered members of <em>C. flaccumfaciens</em>. Hence, the latter two groups were elevated at the species level as <em>C. poinsettiae</em> sp. nov. (ATCC 9682^T = CFBP 2403^T = ICMP 2566^T = LMG 3715^T = NCPPB 854^T as type strain), and <em>C. aurantiacum</em> sp. nov. (50R^T = CFBP 8819^T = ICMP 22071^T as type strain). Within the emended species <em>C. flaccumfaciens</em> comb. nov., yellow-pigmented strains causing bacterial wilt of dry beans and those causing bacterial canker of <em>Euphorbia pulcherrima</em> in Europe were retained as <em>C. flaccumfaciens</em> pv. <em>flaccumfaciens</em> and <em>C. flaccumfaciens</em> pv<em>. poinsettiae,</em> respectively; while taxonomic position of the sugar beet pathogen <em>C. flaccumfaciens</em> pv. <em>beticola</em> ATCC BAA144^PT was confirmed. The newly described onion pathogen <em>C. allii</em> was also reclassified as <em>C. flaccumfaciens</em> pv<em>. allii</em> with the pathotype strain LMG 32517^PT. Furthermore, <em>C. flaccumfaciens</em> pv. <em>basellae</em> causing bacterial leaf spot of malabar spinach (<em>Basella rubra</em>) was transferred to <em>C. citreum</em> pv. <em>basellae</em> with ATCC BAA143^PT as pathotype.</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139679730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Andean soil-derived lignocellulolytic bacterial consortium as a source of novel taxa and putative plastic-active enzymes","authors":"Laura Díaz-García ,&nbsp;Maria Chuvochina ,&nbsp;Golo Feuerriegel ,&nbsp;Boyke Bunk ,&nbsp;Cathrin Spröer ,&nbsp;Wolfgang R. Streit ,&nbsp;Luis M. Rodriguez-R ,&nbsp;Jörg Overmann ,&nbsp;Diego Javier Jiménez","doi":"10.1016/j.syapm.2023.126485","DOIUrl":"10.1016/j.syapm.2023.126485","url":null,"abstract":"<div><p><span><span>An easy and straightforward way to engineer microbial environmental communities is by setting up liquid enrichment cultures<span> containing a specific substrate as the sole source of carbon. Here, we analyzed twenty single-contig high-quality metagenome-assembled genomes (MAGs) retrieved from a microbial consortium (T6) that was selected by the dilution-to-stimulation approach using Andean soil as </span></span>inoculum<span><span> and lignocellulose<span> as a selection pressure. Based on genomic metrics (e.g., average nucleotide and amino acid identities) and </span></span>phylogenomic analyses, 15 out of 20 MAGs were found to represent novel bacterial species, with one of those (MAG_26) belonging to a novel genus closely related to </span></span><em>Caenibius</em> spp. (<span><em>Sphingomonadaceae</em></span>). Following the rules and requirements of the SeqCode, we propose the name <em>Andeanibacterium colombiense</em> gen. nov., sp. nov. for this taxon. A subsequent functional annotation of all MAGs revealed that MAG_7 (<em>Pseudobacter hemicellulosilyticus</em><span> sp. nov.) contains 20, 19 and 16 predicted genes from carbohydrate-active enzymes families GH43, GH2 and GH92, respectively. Its lignocellulolytic gene profile resembles that of MAG_2 (the most abundant member) and MAG_3858, both of which belong to the </span><span><em>Sphingobacteriaceae</em></span><span> family. Using a database that contains experimentally verified plastic-active enzymes (PAZymes), twenty-seven putative bacterial polyethylene terephthalate (PET)-active enzymes (i.e., alpha/beta-fold hydrolases) were detected in all MAGs. A maximum of five putative PETases were found in MAG_3858, and two PETases were found to be encoded by </span><em>A. colombiense</em><span>. In conclusion, we demonstrate that lignocellulose-enriched liquid cultures coupled with genome-resolved metagenomics are suitable approaches to unveil the hidden bacterial diversity and its polymer-degrading potential in Andean soil ecosystems.</span></p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138561074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Halomonas rhizosphaerae sp. nov. and Halomonas kalidii sp. nov., two novel moderate halophilic phenolic acid-degrading species isolated from saline soil","authors":"Lian Xu ,&nbsp;Hua-Mei Wei ,&nbsp;Ye-Nan Sun ,&nbsp;Qi Wu ,&nbsp;Xiao-Yan Gao ,&nbsp;Biao Shen ,&nbsp;Ji-Quan Sun","doi":"10.1016/j.syapm.2024.126488","DOIUrl":"10.1016/j.syapm.2024.126488","url":null,"abstract":"<div><p><span>Four vanillic acid-degrading bacterial strains, named LR5S13</span>^T, LR5S20, and M4R5S39^T and LN1S58, were isolated from <em>Kalidium cuspidatum</em><span><span><span> rhizosphere and bulk soils, respectively. </span>Phylogenetic analysis based on 16S </span>rRNA gene as well as core genome revealed that LR5S13</span>^T and LR5S20 clustered closely with each other and with <span><em>Halomonas</em><em> ventosae</em></span> Al12^T, and that the two strains shared the highest similarities (both 99.3 %) with <em>H. ventosae</em> Al12^T, in contrast, M4R5S39^T and LN1S58 clustered together and with <em>Halomonas heilongjiangensis</em> 9–2^T, and the two strains shared the highest similarities (99.4 and 99.2 %, respectively) with <em>H. heilongjiangensis</em> 9–2^T. The average nucleotides identities based on BLAST (ANIb) and digital DNA-DNA hybridization (dDDH) values of strains LR5S13^T to LR5S20, and M4R5S39^T<span> to LN1S58, were both higher than the threshold values for delineation of a species. The ANIb and dDDH values of the four strains to their closely relatives were lower than the threshold values. All four strains take phosphatidylethanolamine<span>, phosphatidylglycerol<span>, and diphosphatidylglycerol as the major polar lipids, Summed Feature 8, Summed Feature 3, and C</span></span></span>_16:0 as the major fatty acids. Based on the phylogenetic and phenotypic results, the four strains should be classified as two novel <em>Halomonas</em> species. Therefore, <em>Halomonas rhizosphaerae</em> sp. nov. (type strain LR5S13^T = KCTC 8016^T = CGMCC 1.62049^T) and <em>Halomonas kalidii</em> (type strain M4R5S39^T = KCTC 8015^T = CGMCC 1.62047^T<span>) are proposed. The geographical distribution analysis based on 16S rRNA gene revealed that the two novel species are widely distributed across the globe, specifically in highly saline habits, especially in Central and Eastern Asia.</span></p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139517363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First single-strain enrichments of Electrothrix cable bacteria, description of E. aestuarii sp. nov. and E. rattekaaiensis sp. nov., and proposal of a cable bacteria taxonomy following the rules of the SeqCode","authors":"Lea E. Plum-Jensen,&nbsp;Andreas Schramm,&nbsp;Ian P.G. Marshall","doi":"10.1016/j.syapm.2024.126487","DOIUrl":"10.1016/j.syapm.2024.126487","url":null,"abstract":"<div><p>Cable bacteria are electrically conductive, filamentous Desulfobulbaceae, which are morphologically, functionally, and phylogenetically distinct from the other members of this family. Cable bacteria have not been obtained in pure culture and were therefore previously described as candidate genera, <em>Candidatus</em> Electrothrix and <em>Ca.</em> Electronema; a representative of the latter is available as single-strain sediment enrichment. Here we present an improved workflow to obtain the first single-strain enrichments of <em>Ca.</em> Electrothrix and report their metagenome-assembled genomes (MAGs) and morphology. Based on these results and on previously published high-quality MAGs and morphological data of cable bacteria from both candidate genera, we propose to adopt the genus names <em>Electrothrix</em> and <em>Electronema</em> following the rules of the Code of Nomenclature of Prokaryotes Described from Sequence Data (SeqCode), with <em>Electrothrix communis</em> RB^TS and <em>Electronema aureum</em> GS^TS, respectively, as the nomenclatural types of the genera. Furthermore, based on average nucleotide identity (ANI) values &lt; 95 % with any described species, we propose two of our three single-strain enrichment cultures as novel species of the genus <em>Electrothrix</em>, with the names <em>E. aestuarii</em> sp. nov. and <em>E. rattekaaiensis</em> sp. nov., according to the SeqCode.</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0723202024000018/pdfft?md5=bf0854542f33b2bd8f04b55b6069e5f4&pid=1-s2.0-S0723202024000018-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139583548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Halapricum hydrolyticum sp. nov., a beta-1,3-glucan utilizing haloarchaeon from hypersaline lakes” [Syst. Appl. Microbiol. 46(6) (2023) 126471]","authors":"Dimitry Y. Sorokin ,&nbsp;Alexander G. Elcheninov ,&nbsp;Alexander Y. Merkel ,&nbsp;Nicole J. Bale ,&nbsp;Jaap Sininghe-Damste ,&nbsp;Ilya V. Kublanov","doi":"10.1016/j.syapm.2023.126475","DOIUrl":"10.1016/j.syapm.2023.126475","url":null,"abstract":"","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S072320202300084X/pdfft?md5=b08dbeb76b153e9b8d1c6430271f412f&pid=1-s2.0-S072320202300084X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138470939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In the footsteps of Heinz Schlesner and Peter Hirsch: Exploring the untapped diversity of the phylum Planctomycetota in isolates from the 1980s to the early 2000s","authors":"Nicolai Kallscheuer ,&nbsp;Carmen E. Wurzbacher ,&nbsp;Ruth A. Schmitz ,&nbsp;Christian Jogler","doi":"10.1016/j.syapm.2023.126486","DOIUrl":"10.1016/j.syapm.2023.126486","url":null,"abstract":"<div><p>Recent sampling and strain isolation campaigns have accelerated research on the bacterial phylum <em>Planctomycetota</em>. The contribution of more than 100 novel isolates to the open collection of currently 123 described planctomycetal species in the last decade benefited greatly from pioneering work conducted in the second half of the last century. One of those pioneers was Heinz Schlesner, who investigated budding and prosthecate bacteria from habitats world-wide during his time at Christian-Albrechts-University Kiel. An outcome of his research was a strain collection with more than 500 isolates belonging to different bacterial phyla, many of which are uncharacterised members of the phylum <em>Planctomycetota</em>. Due to the lack of affordable genome sequencing techniques at the time of their isolation, most of them were characterised based on phenotypic features and DNA-DNA hybridisation experiments. After the retirement of Heinz Schlesner in 2002, the collection was stored for several years and transferred to Jena in 2019. To get a glimpse on the diversity of members from the phylum <em>Planctomycetota</em> in Schlesner’s collection, we here summarised from his records and publications all available information about the collection regarding sampling habitat and phylogeny. Furthermore, we conducted an updated phylogenetic analysis for a representative excerpt of the collection based on the 16S rRNA gene sequence of 59 strains Schlesner deposited in the NCBI database during strain characterisation studies published in the 1980s until the early 2000s. The results support that strains from his collection are still a valuable contribution to expand the cultivated diversity of the understudied phylum <em>Planctomycetota</em>.</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0723202023000954/pdfft?md5=198f1fcaa4217bb2eebb6bb62ff3f810&pid=1-s2.0-S0723202023000954-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138683869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multilocus sequence and phenotypic analysis of Pectobacterium and Dickeya type strains for identification of soft rot Pectobacteriaceae from symptomatic potato stems and tubers in Pennsylvania","authors":"Amanda M. Mainello-Land ,&nbsp;Shaheen Bibi ,&nbsp;Beth Gugino ,&nbsp;Carolee T. Bull","doi":"10.1016/j.syapm.2023.126476","DOIUrl":"10.1016/j.syapm.2023.126476","url":null,"abstract":"<div><p>Outbreaks of potato blackleg and soft rot caused by <span><em>Pectobacterium</em></span> species and more recently <span><em>Dickeya</em></span><span> species across the U.S.<span> mid-Atlantic region have caused yield loss due to poor emergence as well as losses from stem and tuber rot. To develop management strategies for soft rot diseases, we must first identify which members of the soft rot </span></span><em>Pectobacteriaceae</em> are present in regional potato plantings. However, the rapidly expanding number of soft rot <em>Pectobacteriaceae</em> species and the lack of readily available comparative data for type strains of <em>Pectobacterium</em> and <em>Dickeya</em> hinder quick identification. This manuscript provides a comparative analysis of soft rot <em>Pectobacteriaceae</em><span> and a comprehensive comparison of type strains from this group using rep-PCR, MLSA and 16S sequence analysis, as well as phenotypic and physiological analyses using Biolog GEN III plates. These data were used to identify isolates cultured from symptomatic potato stems collected between 2016 and 2018. The isolates were characterized for phenotypic traits and by sequence analysis to identify the bacteria from potatoes with blackleg and soft rot symptoms in Pennsylvania potato fields. In this survey, </span><span><em>P. actinidiae, P. brasiliense, P. polonicum, P. </em><em>polaris</em><em>, P. punjabense, P. parmentieri,</em></span> and <em>P. versatile</em> were identified from Pennsylvania for the first time. Importantly, the presence of <em>P. actinidiae</em> in Pennsylvania represents the first report of this organism in the U.S. As expected, <em>P. carotorvorum</em> and <em>D. dianthicola</em> were also isolated. In addition to a resource for future work studying the <em>Dickeya</em> and <em>Pectobacterium</em> associated with potato blackleg and soft rot, we provide recommendations for future surveys to monitor for quarantine or emerging soft rot <em>Pectobacteriace</em> regionally.</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2023-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138534230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel method for the whole-cell detection of environmental microorganisms using hemin and tyramide signal amplification (Hemin-TSA) with a desired fluorescent dye","authors":"Kampachiro Urasaki ,&nbsp;Yu-You Li ,&nbsp;Kengo Kubota","doi":"10.1016/j.syapm.2023.126473","DOIUrl":"https://doi.org/10.1016/j.syapm.2023.126473","url":null,"abstract":"<div><p><span>A method called hemin-tyramide signal amplification (Hemin-TSA) was developed for visualization of environmental microorganisms using hemin and tyramide signal amplification. In Hemin-TSA, hemin, which has </span>peroxidase<span><span> activity, is bound to microbial cells, and a desired fluorescent dye is deposited on the microbial cells by a hemin-catalyzed TSA reaction. The protocol was initially optimized in terms of hemin concentration, hemin binding time and repeated reaction times of TSA. Hemin-TSA showed a comparative or improved signal-to-noise ratio compared to DAPI staining. The shapes of fluorescent signals obtained from microbial cells were almost morphologically identical to those observed in phase contrast microscopy. Hemin-TSA staining provided more accurate cell counts than DAPI staining, especially for actively growing cells, for which two or three spotty DAPI signals were obtained from a single cell. In addition, microbial cells that were not detected by DAPI staining were detected by Hemin-TSA with fluorescein, which enabled us to avoid high non-specific fluorescence under UV excitation. The method developed in this study allows us to visually detect microbial cells in various environments with the characteristics of better cell morphological identification, improved enumeration accuracy and </span>selectivity of fluorescent dyes.</span></p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134655286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Halapricum hydrolyticum sp. nov., a beta-1,3-glucan utilizing haloarchaeon from hypersaline lakes","authors":"Dimitry Y. Sorokin ,&nbsp;Alexander G. Elcheninov ,&nbsp;Alexander Y. Merkel ,&nbsp;Nicole J. Bale ,&nbsp;Jaap Sininghe-Damste ,&nbsp;Ilya V. Kublanov","doi":"10.1016/j.syapm.2023.126471","DOIUrl":"10.1016/j.syapm.2023.126471","url":null,"abstract":"<div><p><span>Two strains of neutrophilic haloaloarchaea were selectively enriched from hypersaline lakes in southwestern Siberia<span> using β-1,3-glucans as a substrate. The strains were nearly identical in their phenotypes and according to phylogenomic analysis, and represent a distant novel species group in the genus </span></span><em>Halapricum</em> of the family <em>Haloarculaceae.</em><span><span> The main phenotypic property of the novel isolates is the ability to hydrolyze and grow with the </span>polysaccharides<span><span><span><span> curdlan and pachyman. Such potential has, to date, not been seen in any other </span>haloarchaea in pure cultures. The strains are obligately aerobic saccharolytics. Apart from the insoluble β-1,3-glucans, they utilized soluble α-glucans (starch, </span>pullulan and glycogen) and a limited number of sugars. The major ether-bound polar </span>phospholipids<span><span> include PGP-Me and PG. The glyco- and sulfolipids were absent. The major respiratory </span>menaquinone is MK-8:8. On the basis of their unique physiological properties and the results of phylogenomic analysis, the isolates are suggested to be classified into a novel species </span></span></span><em>Halapricum hydrolyticum</em> sp. nov. (type strain HArc-curdl5-1^T = DSM 114193^T = UQM 41587^T).</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2023-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41213588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}