Marike Palmer , Nancy O. Nou , Lara A. Turello , Carolyn E. Power , John C. Ong , Jonathan K. Covington , Dengxun Lai , Xavier Mayali , Anna-Louise Reysenbach , Yan-Ling Qi , Jeremy A. Dodsworth , Jian-Yu Jiao , Lan Liu , Zheng-Han Lian , Meng-Meng Li , Yitai Liu , Ranjani Murali , Brandon R. Briggs , Hongyan Zuo , Stephen R. Quake , Brian P. Hedlund
{"title":"Nitrogen fixation in Pampinifervens, a new species-rich genus of Aquificaceae that inhabits a wide pH range in terrestrial hot springs","authors":"Marike Palmer , Nancy O. Nou , Lara A. Turello , Carolyn E. Power , John C. Ong , Jonathan K. Covington , Dengxun Lai , Xavier Mayali , Anna-Louise Reysenbach , Yan-Ling Qi , Jeremy A. Dodsworth , Jian-Yu Jiao , Lan Liu , Zheng-Han Lian , Meng-Meng Li , Yitai Liu , Ranjani Murali , Brandon R. Briggs , Hongyan Zuo , Stephen R. Quake , Brian P. Hedlund","doi":"10.1016/j.syapm.2025.126644","DOIUrl":"10.1016/j.syapm.2025.126644","url":null,"abstract":"<div><div>Members of the <em>Aquificota</em> play important roles in hydrogen, sulfur, and carbon cycles in geothermal systems as ubiquitous and abundant chemolithoautotrophs; however, their roles in the nitrogen cycle are poorly defined. Here, we show that <em>Aquificaceae</em> isolate T-2<sup>T</sup> from Tengchong, China, fixes nitrogen based on growth with dinitrogen as the sole nitrogen source and incorporation of <sup>15</sup>N atoms when grown with <sup>15</sup>N<sub>2</sub>. We further show evidence that suggests its nitrogen fixation (<em>nif</em>) genes are transcribed in situ in a nearby spring. We propose the name <em>Pampinifervens diazotrophicum</em> gen. et sp. nov., with strain T-2<sup>T</sup> (= JCM 35475<sup>T</sup> = DSM 116324<sup>T</sup>) as the nomenclatural type. Its close relative designated <em>Pampinifervens florentissimum</em> sp. nov. T-8<sup>T</sup> (= CGMC 1.5214<sup>T</sup> = JCM 33569<sup>T</sup>) does not fix nitrogen but respires nitrate. Analysis of 104 metagenome-assembled genomes from 84 metagenomes allowed us to circumscribe the genus <em>Pampinifervens</em> and identify at least 16 sympatric <em>Pampinifervens</em> species that dominate the <em>Aquificaceae</em> populations in diverse hot springs in southwest China, with distinct species globally. Analysis of these <em>Pampinifervens</em> genomes revealed variable presence of <em>nif</em> and denitrification genes and frequent gene loss and horizontal transfer. Genes for pH homeostasis were also present in <em>Pampinifervens</em> genomes, with a proliferation in species from Tengchong, consistent with the wide pH growth range of both <em>Pampinifervens</em> isolates and the prevalence of <em>Pampinifervens</em> in both alkaline and acidic springs. The dynamic evolution of <em>nif</em> and denitrification genes, along with pH homeostasis genes, may facilitate the diversification of <em>Pampinifervens</em> into diverse springs with different nitrogen availability.</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 5","pages":"Article 126644"},"PeriodicalIF":4.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144749143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Astrobacterium formosum gen. nov., sp. nov., a novel psychrotolerant rosette-forming bacterium from a laboratory psychrophilic EGSB bioreactor","authors":"V.V. Potokina , S.N. Parshina , A.Y. Merkel , A.G. Elcheninov , A.V. Vishnyakova , A.N. Avtukh , N.A. Kostrikina , V.M. Tereshina , Y.V. Litti","doi":"10.1016/j.syapm.2025.126640","DOIUrl":"10.1016/j.syapm.2025.126640","url":null,"abstract":"<div><div>A new psychrotolerant, facultative anaerobic bacterium, strain Astr-EGSB<sup>T</sup>, was isolated from a syntrophic propionate-oxidizing consortium obtained from a low-temperature expanded granular sludge bed (EGSB) bioreactor fed with a mixture of volatile fatty acids. The cells of this strain were Gram-negative and non-spore-forming rods (0.5<strong>·</strong>0.8–1.5<strong>·</strong>9 μm), grew either as individual cells or formed rosettes of various sizes, resembling stars. Bacteria reproduced by budding, and each cell has a sticky, non-reproductive pole that allows them to attach to other cells, forming rosette-like clusters. This strain could grow between 5 and 40 °C, with pH ranging from 4.5 to 7.5 and NaCl concentration of 0 to 2 %. It could degrade a variety of carbon substrates, including sugars, alcohols, casamine acids and several organic acids, but could not grow under photoautotrophic conditions. During glucose fermentation, the products include lactate, acetate, isobutyrate, CO<sub>2</sub>, and traces of succinate. Major cellular fatty acids were C<sub>18:1</sub>ω9c and C<sub>19:0</sub> cyclo <em>ω</em>9c, menaquinone МХ-10(H10). Main polar lipids were phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. Sequence comparison of the 16S rRNA gene revealed that strain Astr-EGSB<sup>T</sup> was related to <em>Pseudorhodoplanes sinuspersici</em> RIPI 110<sup>T</sup> with a similarity of 95.0 %. Based on phylogenomic analysis using 120 marker genes, strain Astr-EGSB<sup>T</sup> belongs to the distinct genus-level phylogenetic lineage that is most related to the genus <em>Rhodoplanes</em>. The G + C content of genomic DNA was 65.8 mol%. Based on genomic and physiological characteristics of strain Astr-EGSB<sup>T</sup>, a new genus and a new species, <em>Astrobacterium formosum</em> gen. Nov., sp. nov., is proposed, with Astr-EGSB<sup>T</sup> as the type strain.</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 5","pages":"Article 126640"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sana Bari , Yuwei Zhang , Valery Patsekin , J. Paul Robinson , Bartek Rajwa , Andrew Gehring , James Lindsay , Don Kulasiri , Stephen L.W. On
{"title":"Assessment of cluster analysis of elastic light scatter profiles for the identification of foodborne Bacteria","authors":"Sana Bari , Yuwei Zhang , Valery Patsekin , J. Paul Robinson , Bartek Rajwa , Andrew Gehring , James Lindsay , Don Kulasiri , Stephen L.W. On","doi":"10.1016/j.syapm.2025.126641","DOIUrl":"10.1016/j.syapm.2025.126641","url":null,"abstract":"<div><div>Elastic Light Scatter (ELS) profiling is a novel approach for simultaneous detection and identification of bacteria cultured on solid agar media. The profiles comprise a range of different scatter features that can be used jointly or individually as a basis for comparison. We examined the utility of cluster analysis of ELS profiles for classification and identification of bacteria of relevance to foods. A total of 1562 colonies from 48 strains, representing 17 different species distributed among four genera, were examined. Each of three scatter-derived features (Zernike moments, pseudo-Zernike moments, proprietary Patsekin elements) were used individually and in combination for the cluster analysis. Of these, a combination of Patsekin elements and pseudo-Zernike moments yielded clusters that best reflected the known taxonomic relationships among the strains examined. Evidence of Genus-level markers of colony architecture was seen and there was a general agreement of clustering at the species level. Nonetheless, some individual colonies did not cluster with the majority of others from the same taxon, which could reflect an aberrant ELS phenotype, or known challenges in depicting strain relationships using cluster analytical methods. However, when compared with UMAP data processing, relationships between individual colonies were more easily discerned by inspecting the dendrogram. Cluster analysis of ELS profiles is a useful adjunctive tool for the classification and identification of bacteria and results may also be helpful in informing the development and improvement of other data analytical tools for ELS profile analysis.</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 5","pages":"Article 126641"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144686304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Galina B. Slobodkina , Alexander Y. Merkel , Kseniya V. Kondrasheva , Alexandra R. Stroeva , Elizaveta A. Bonch-Osmolovskaya , Kahramon D. Davranov , Alexander I. Slobodkin
{"title":"Taxonomic and metabolic diversity of microbial communities in a thermal water stream in Uzbekistan and proposal of two new classes of uncultivated bacteria, Desulfocorpusculia class. nov. and Tepidihabitantia class. nov., named following the rules of SeqCode","authors":"Galina B. Slobodkina , Alexander Y. Merkel , Kseniya V. Kondrasheva , Alexandra R. Stroeva , Elizaveta A. Bonch-Osmolovskaya , Kahramon D. Davranov , Alexander I. Slobodkin","doi":"10.1016/j.syapm.2025.126650","DOIUrl":"10.1016/j.syapm.2025.126650","url":null,"abstract":"<div><div>Thermal ecosystems in Uzbekistan remain poorly characterized, particularly through culture-independent approaches. In this study, we performed 16S rRNA gene metabarcoding and metagenomic sequencing of microbial communities from a hot stream formed by the discharge of thermal artesian groundwater in the Navoiy region. The taxonomic composition of microbial communities varied with temperature and sample type, with the phylum <em>Chloroflexota</em> abundant in most samples. Members of <em>Aquificota</em>, <em>Deinococcota,</em> and <em>Thermotogota</em> dominated in sediments with temperatures around 60 °C, while <em>Desulfobacterota</em> and <em>Cyanobacteriota</em> were more abundant at lower temperatures. Metagenomic sequencing of the microbial community in sediment under orange-brown mat (54 °C) revealed a dominance of the phyla <em>Chloroflexota, Armatimonadota, Aquificota, Ignavibacteriota, Desulfobacterota</em> and <em>Bacteroidota</em>. Metagenomic data indicated that fermentation, aerobic degradation of organic matter, hydrogen oxidation, and sulfur cycling are the main metabolic processes. Two high-quality metagenome-assembled genomes (MAGs) were described as novel taxa named following the rules of SeqCode. Genome analysis suggests that both organisms are heterotrophic anaerobes capable of sulfate reduction or fermentation. We provide the description of <em>Desulfocorpusculum asiaticum</em><sup>Ts</sup> gen. nov., sp. nov. affiliated with the novel family <em>Desulfocorpusculaceae</em> fam. nov., order <em>Desulfocorpusculales</em> ord. nov. and class <em>Desulfocorpusculia</em> class. nov.; and the description of the new species and genus <em>Tepidihabitans asiaticus</em><sup>Ts</sup> gen. nov., sp. nov. affiliated with the novel family <em>Tepidihabitantaceae</em> fam. nov., order <em>Tepidihabitantales</em> ord. nov. and class <em>Tepidihabitantia</em> class. nov.</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 5","pages":"Article 126650"},"PeriodicalIF":4.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144921308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Olga A. Podosokorskaya , Alexander Y. Merkel , Andrei A. Novikov , Alexandra A. Klyukina , Alexander G. Elcheninov
{"title":"Pyranulibacter aquaticus gen. nov., sp. nov., the first nitrate-reducing representative of the class Ignavibacteria from an Iturup well","authors":"Olga A. Podosokorskaya , Alexander Y. Merkel , Andrei A. Novikov , Alexandra A. Klyukina , Alexander G. Elcheninov","doi":"10.1016/j.syapm.2025.126639","DOIUrl":"10.1016/j.syapm.2025.126639","url":null,"abstract":"<div><div>A novel facultatively anaerobic moderately thermophilic bacterium, strain 4301-Me<sup>T</sup> was isolated from the well 13k on the Iturup Island (Kuril Islands, Russian Federation). Gram-stain-negative, motile rods were present singly, in pairs, rosettes, and aggregates, or formed biofilms. The strain grew optimally at 50–55 °C, pH 6.7–7.0 and 0.2–0.4 % NaCl. It did not require yeast extract for growth and utilized mono-, di- and polysaccharides (cellulose, starch, xanthan gum, locust bean gum, glucomannan, lichenan, amorphous chitin) as well as proteinaceous substrates (gelatin, peptone, beef and yeast extract). Growth under anaerobic conditions was observed in the presence and absence of external electron acceptors. Sulfur, thiosulfate, nitrate, arsenate, Fe-citrate, and ferrihydrite were reduced with acetate, or yeast extract as electron donors. The respiratory quinone was MK-7. Major cellular fatty acids were iso-C<sub>15:0</sub>, iso-C<sub>16:0</sub>, anteiso-C<sub>15:0</sub>, 3OH-C<sub>14:0</sub>, 3OH-C<sub>13:0</sub>. The size of the genome and genomic DNA G + C content of strain 4301-Me<sup>T</sup> were 3.34 Mb and 33 %, respectively. According to the 16S rRNA gene sequence and conserved protein sequences phylogenies, strain 4301-Me<sup>T</sup> represented a distinct lineage of the family <em>Melioribacteraceae</em> within the class <em>Ignavibacteria</em>. Based on phylogenomic analysis and phenotypic features, the novel isolate was assigned to a novel genus, for which the name <em>Pyranulibacter</em> gen. nov. is proposed. Strain 4301-Me<sup>T</sup> represents its type species <em>Pyranulibacter aquaticus</em> sp. nov. (=CGMCC 1.18061<sup>T</sup> = B-3822<sup>T</sup> = BIM B-2047<sup>T</sup> = UQM 41592<sup>T</sup>).</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 5","pages":"Article 126639"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144623499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Juliana Botero, Margo Cnockaert, Yakun Zhang, Charlotte Peeters, Peter Vandamme
{"title":"Carnimonas bestiolae sp. nov. and Cernens ardua gen. nov., sp. nov.: new halotolerant bacteria from the invasive solitary bee Megachile sculpturalis","authors":"Juliana Botero, Margo Cnockaert, Yakun Zhang, Charlotte Peeters, Peter Vandamme","doi":"10.1016/j.syapm.2025.126648","DOIUrl":"10.1016/j.syapm.2025.126648","url":null,"abstract":"<div><div>Fifteen isolates from gut samples of the invasive solitary bee <em>Megachile sculpturalis</em> remained unidentified after matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis. Phylogenomic and overall genome relatedness indices analyses of five representative isolates demonstrated that 14 isolates represented a novel <em>Carnimonas</em> species for which we propose the name <em>Carnimonas bestiolae</em> sp. nov., with LMG 33810<sup>T</sup> as the type strain. A single isolate represented a distinct lineage within the <em>Halomonadaceae</em> family with the genera <em>Carnimonas</em> and <em>Halotalea</em> as nearest neighbor taxa and is classified as <em>Cernens ardua</em> gen. nov., sp. nov., with LMG 33818<sup>T</sup> as the type strain. Comparative genomic analysis and physiological characterization revealed functional differences between <em>C. bestiolae, Ce. ardua</em> and established <em>Carnimonas</em> and <em>Halotalea</em> species in central metabolism, fatty acid metabolism, and osmoregulatory pathways, the latter being consistent with adaptation to a saline environment. A reanalysis of previously published insect microbiome studies in which <em>Carnimonas</em> was reported, revealed 16S rRNA amplicon sequence variants with >99 % identity to <em>C. bestiolae</em> LMG 33810<sup>T</sup> 16S rRNA in gut samples of <em>Megachile sculpturalis</em>, <em>Anthidium florentinum</em>, and the psyllid <em>Leptynoptera sulfurea</em>. In contrast, 16S rRNA amplicon sequence variants corresponding to <em>Ce. ardua</em> were rarely detected and only at low relative abundances, suggesting a transient association with the insect gut.</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 5","pages":"Article 126648"},"PeriodicalIF":4.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144878890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Peter Vandamme , Charlotte Peeters , Amanda Hettiarachchi , Margo Cnockaert , Aurélien Carlier
{"title":"Corrigendum to “Govania unica gen. nov., sp. nov., a rare biosphere bacterium that represents a novel family in the class Alphaproteobacteria” [Syst. Appl. Microbiol. 46(3) (2023) 126405]","authors":"Peter Vandamme , Charlotte Peeters , Amanda Hettiarachchi , Margo Cnockaert , Aurélien Carlier","doi":"10.1016/j.syapm.2025.126642","DOIUrl":"10.1016/j.syapm.2025.126642","url":null,"abstract":"","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 5","pages":"Article 126642"},"PeriodicalIF":4.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144733375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kamlesh Jangid , Wen-Jun Li , Stephanus Venter , Wee Fei Aaron Teo , William Whitman
{"title":"BISMiS Live: a digital platform for global communication among microbial systematists","authors":"Kamlesh Jangid , Wen-Jun Li , Stephanus Venter , Wee Fei Aaron Teo , William Whitman","doi":"10.1016/j.syapm.2025.126646","DOIUrl":"10.1016/j.syapm.2025.126646","url":null,"abstract":"<div><div>BISMiS Live, launched on March 20, 2021, emerged as a timely response to the disruption caused by the CoViD-19 pandemic, offering a global virtual platform for discourse in microbial systematics. Spearheaded by the Bergey's International Society for Microbial Systematics (BISMiS), this monthly webinar series hosted renowned experts and engaged thousands of participants across 84 countries. The initiative emphasized inclusivity, scientific depth, and digital accessibility, with sessions archived on YouTube (@<span><span>BISMiS_</span><svg><path></path></svg></span>) for broader impact. Behind each event lies a dedicated team managing speaker coordination, technical execution, and global outreach. BISMiS Live has grown into an educational resource, integrated into university curricula and inspiring the next generation of microbial taxonomists. With suggestions for future innovation, such as student lightning talks, thematic series, and podcast formats, the platform is poised to evolve further. BISMiS Live stands as a digital bridge connecting systematists worldwide, sustaining knowledge exchange and community through innovation and collaboration.</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 5","pages":"Article 126646"},"PeriodicalIF":4.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144864605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hao-hao Wang , Chi Zhang , Ya-qin Chen , Yi-fan Guo , Li-jian Ding , Sheng-fa Zhang , Gai-jun Du , Wei-yan Zhang , Shan He
{"title":"Genomic characterization of Gillisia xinjiangensis sp. nov.: insights into nitrogen metabolism, stress adaptation, and application potential","authors":"Hao-hao Wang , Chi Zhang , Ya-qin Chen , Yi-fan Guo , Li-jian Ding , Sheng-fa Zhang , Gai-jun Du , Wei-yan Zhang , Shan He","doi":"10.1016/j.syapm.2025.126645","DOIUrl":"10.1016/j.syapm.2025.126645","url":null,"abstract":"<div><div>High-altitude saline lakes host distinctive microbial communities characterized by specialized adaptations to extreme environmental conditions. However, the underlying mechanisms enabling bacterial survival and physiological function in these habitats remain largely unexplored. In this study, a novel Gram-stain-negative, non-motile, rod-shaped bacterium, designated strain Q332<sup>T</sup>, was isolated from sediment samples collected from Achikkul Lake, a high-altitude saline lake in Xinjiang, northwestern China. The strain was facultatively aerobic, and grew optimally at 28 °C, pH 7.0, and 3.0 % (<em>w</em>/<em>v</em>) NaCl. Catalase and oxidase activities were positive. Major fatty acids identified in strain Q332<sup>T</sup> included anteiso‐C<sub>15:0</sub>, anteiso-C<sub>17:1</sub>, iso‐C<sub>15:0</sub> and C<sub>16:0</sub>. The primary respiratory quinone detected was menaquinone-6 (MK-6). Polar lipid analyses identified phosphatidylethanolamine (PE), one unidentified acyloxyacyl phosphatidylglycerol (APGL), three unidentified lipids (L), three glycolipids (GL), one phospholipid (PL), and four amidolipids (AL). The genomic DNA G + C content is 38.9 %. Genomic characterization revealed a versatile metabolic repertoire, including unique C1-unit metabolic pathways and comprehensive nitrogen cycling capabilities, notably denitrification and assimilatory nitrate reduction. Furthermore, strain Q332<sup>T</sup> displayed robust mechanisms for stress adaptation, encompassing tolerance to cold and osmotic stress, defenses against oxidative stress, and resistance to heavy metals. Based on phenotypic, chemotaxonomic, phylogenetic, and genomic features, strain Q332<sup>T</sup> is considered to represent a novel species of the genus <em>Gillisia</em>, for which the name <em>Gillisia xinjiangensis</em> sp. nov. is proposed. The type strain is Q332<sup>T</sup> (=KCTC 102200<sup>T</sup> = MCCC 1K09490<sup>T</sup>).</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 5","pages":"Article 126645"},"PeriodicalIF":4.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144864606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Erratum to “Polyphasic taxonomic description of Streptomyces tritrimontium sp. nov., actinomycetes isolated from moonmilk in Szczelina Chochołowska cave of Tatra Mountains in Poland and emended descriptions of Streptomyces manipurensis Nimaichand et al. 2021 and Streptomyces nojiriensis Ishida et al. 1967” [Syst. Appl. Microbiol. 48 (2025) 126627]","authors":"Patrycja Bielańska , Patrycja Golińska , Elaine Romines , Łukasz Rąbalski , Grzegorz Węgrzyn","doi":"10.1016/j.syapm.2025.126647","DOIUrl":"10.1016/j.syapm.2025.126647","url":null,"abstract":"","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 5","pages":"Article 126647"},"PeriodicalIF":4.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145006601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}