Methyloraptor flagellatus gen.Nov.,sp. nov.,以甲烷球菌属养甲烷菌为食的隶属于 Ancalomicrobiaceae 的新型兼性滋养甲基细菌

IF 3.3 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Victoria A. Saltykova , Olga V. Danilova , Igor Y. Oshkin , Svetlana E. Belova , Natalia E. Suzina , Nikolai V. Pimenov , Svetlana N. Dedysh
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引用次数: 0

摘要

在一个以甲烷球菌属的甲烷营养细菌为生长基质、以连续模式运行的生物反应器中,发现了一种形态明显的微生物联合体。这种联合体由球形的甲基球菌细胞和拉长的杆状菌组成,拉长的杆状菌产生莲座状聚集,抑制了培养过程。从这些细菌中分离出的菌株 S20T,经鉴定属于α-蛋白细菌科 Ancalomicrobiaceae,但与该科的特征成员仅有很远的关系(16S rRNA 基因序列相似度为 93.9-95.1 %)。菌株 S20T 由需氧、运动、兼性甲基营养细菌组成,生长温度为 10 至 45 °C(最适温度为 30 至 35 °C),pH 值范围为 4.5 至 8.5(最适 pH 值为 6.0)。这些细菌能够附着在甲基球菌细胞上,破坏甲烷营养细胞壁的完整性,可能是为了吸食甲醇。与甲基单胞菌也观察到了同样的相互作用。菌株 S20T 的完整基因组序列包括一条 5.0 Mb 的染色体和一条 0.26 Mb 的质粒;DNA G + C 含量为 68.4%。基因组编码 3 个 rRNA 操作子和约 4400 个蛋白质,包括 MxaFI 和 XoxF 类甲醇脱氢酶、丝氨酸途径的所有酶以及完整的趋化途径、单极多糖粘附素和多种肽酶。其基因组序列与早先描述的 Ancalomicrobiaceae 物种的平均氨基酸相同度为 67.20-69.56%。我们建议将这些细菌归为一个新属和新种,即 Methyloraptor flagellatus gen.新种,模式菌株为 S20T(=KCTC 8649T = VKM B-3853T)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Methyloraptor flagellatus gen. Nov., sp. nov., novel Ancalomicrobiaceae-affiliated facultatively methylotrophic bacteria that feed on methanotrophs of the genus Methylococcus
A morphologically conspicuous microbial association was detected in a bioreactor running in a continuous mode with methanotrophic bacteria of the genus Methylococcus and natural gas as a growth substrate. The association consisted of spherical Methylococcus cells colonized by elongated rods, which produced rosette-like aggregates and inhibited the cultivation process. An isolate of these bacteria, strain S20T, was obtained and identified as belonging to the alphaproteobacterial family Ancalomicrobiaceae but displaying only a distant relationship (93.9–95.1 % 16S rRNA gene sequence similarity) to characterized members of this family. Strain S20T was represented by aerobic, motile, facultatively methylotrophic bacteria, which grew between 10 and 45 °C (optimum 30–35 °C) in a pH range of 4.5–8.5 (optimum pH 6.0). These bacteria were capable of attaching to Methylococcus cells and breaking the integrity of methanotroph cell walls, presumably to feed on methanol. The same interaction was observed with Methylomonas species. The finished genome sequence of strain S20T consisted of a 5.0 Mb chromosome and one plasmid, 0.26 Mb in size; the DNA G + C content was 68.4 %. The genome encoded 3 rRNA operons and ~ 4400 proteins including MxaFI- and XoxF-like methanol dehydrogenases, all enzymes of the serine pathway as well as a complete chemotaxis pathway, a unipolar polysaccharide adhesin, and a wide range of peptidases. The genome sequence displayed 67.20–69.56 % average amino acid identity to those of earlier described Ancalomicrobiaceae species. We propose to classify these bacteria as representing a novel genus and species, Methyloraptor flagellatus gen. Nov., sp. nov., with the type strain S20T (=KCTC 8649T = VKM B-3853T).
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来源期刊
Systematic and applied microbiology
Systematic and applied microbiology 生物-生物工程与应用微生物
CiteScore
7.50
自引率
5.90%
发文量
57
审稿时长
22 days
期刊介绍: Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
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