Plant Biology最新文献_第6页

The impact of seasonal variations in inner bark width and xylem growth on irreversible annual stem diameter measurements. 内层树皮宽度和木质部生长的季节变化对不可逆的年度茎粗测量的影响。

IF 3.4 3区生物学

Plant Biology Pub Date : 2026-08-25 DOI: 10.1111/plb.70281

J Gričar, K Eler

{"title":"The impact of seasonal variations in inner bark width and xylem growth on irreversible annual stem diameter measurements.","authors":"J Gričar, K Eler","doi":"10.1111/plb.70281","DOIUrl":"https://doi.org/10.1111/plb.70281","url":null,"abstract":"To better interpret continuous xylem growth measurements from dendrometers, we evaluated how the widths of developing xylem and phloem, along with seasonal trends in inner bark width (conducting phloem, non-conducting phloem and the youngest periderm), contribute to irreversible annual stem diameter changes in Pinus nigra, Fraxinus ornus and Ostrya carpinifolia. Histometric analyses were performed on microcores taken from tree stems in 2020. The seasonal patterns of xylem and phloem formation, as well as changes in inner bark width and xylem growth, varied both within and among species. Of the total annual stem radial increment, xylem and phloem increments accounted for 40.5% and 8.1% in P. nigra, 9.6% and 2.5% in F. ornus, and 17.6% and 7.1% in O. carpinifolia, respectively. The remaining portion comprised inner bark tissues, with non-conducting phloem forming the largest fraction and thus exerting a major influence on variation in inner bark width. Despite seasonal variation in inner bark width, its width at the beginning and end of the growing season was similar across all species, indicating that subtracting inner bark width from the annual stem radial increment at the end of the growing season provides a reasonable estimate of annual xylem increment. However, seasonal changes in inner bark width differed from those of xylem growth, suggesting that xylem phenology cannot be reliably assessed without considering its ontogeny at the cellular level.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148816827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant endemism of the Balkan high-mountain fens: a community-level synthesis and their climate change perspective. 巴尔干高山沼泽的植物特有种：群落水平的综合及其气候变化视角。

IF 3.4 3区生物学

Plant Biology Pub Date : 2026-08-16 DOI: 10.1111/plb.70279

M Hájek, P Hájková, M Chobanova, Z Plesková, E Bergmeier, P Lazarević, D Szokala, N Berisha, L Shuka, D Dítě, T Peterka, R Čusterevska, D Sopotlieva, J Divíšek, I Goia, B Vrábel, Đ Milanović, S Hristovski, D Stešević, P Bureš, I Apostolova

{"title":"Plant endemism of the Balkan high-mountain fens: a community-level synthesis and their climate change perspective.","authors":"M Hájek, P Hájková, M Chobanova, Z Plesková, E Bergmeier, P Lazarević, D Szokala, N Berisha, L Shuka, D Dítě, T Peterka, R Čusterevska, D Sopotlieva, J Divíšek, I Goia, B Vrábel, Đ Milanović, S Hristovski, D Stešević, P Bureš, I Apostolova","doi":"10.1111/plb.70279","DOIUrl":"https://doi.org/10.1111/plb.70279","url":null,"abstract":"The phenomenon of Balkan high-mountain fen plant endemism, unique within Europe, remains poorly studied. We compiled an original dataset of wetland vegetation-plot data across nine countries where Balkan endemics and subendemics occur. We tested the diversity patterns for endemics, non-endemic habitat specialists of bryophytes and vascular plants and matrix-derived taxa with respect to climate, palaeoclimate, edaphic and topographic factors. We used Spearman correlations, Spatial Autoregressive Error Models, Canonical Correspondence Analysis and Principal Component Analysis of abiotic factors with richness patterns projected using Generalised Additive Models. Two major hotspots of fen endemism, centred around the Rila and Korab Mts, were identified. Species composition of endemics generally follows an east-to-west gradient that coincides with palaeoclimatic patterns. Palaeoclimate was influential for community-scale richness of bryophytes and narrow-range endemics, the latter being positively affected by the Bølling-Allerød precipitation peak and the wet-tundra climate during the glacial. The richness of other groups was affected by recent climate, edaphic factors and topography. Water pH differentiated major vegetation types and affected the richness of matrix-derived taxa and fen bryophytes, but had a minor impact on Balkan endemics, except those associated with serpentinite. Several endemic taxa usually co-exist even in small wetland patches. Palaeoclimate shows that fen endemics evidently experienced drier, warmer summers than today over the last 21,000 years; yet they were more affected by past precipitation. If we conserve Balkan high-mountain fens, their catchment properties and hydrology, and maintain moderate grazing, we increase the likelihood that their endemics will withstand ongoing climate change.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148758770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strawberry micropropagation simplifies endomicrobiome and highlights potential for in vitro biotization with beneficial rhizobacteria. 草莓的微繁殖简化了体内微生物组，并突出了与有益的根瘤菌体外生物化的潜力。

IF 3.4 3区生物学

Plant Biology Pub Date : 2026-08-16 DOI: 10.1111/plb.70278

A M G Sepúlveda, E da Conceição Jesus, Y C Molina, V H Buttrós, L Q Carvalho, M Pasqual, J Dória

{"title":"Strawberry micropropagation simplifies endomicrobiome and highlights potential for in vitro biotization with beneficial rhizobacteria.","authors":"A M G Sepúlveda, E da Conceição Jesus, Y C Molina, V H Buttrós, L Q Carvalho, M Pasqual, J Dória","doi":"10.1111/plb.70278","DOIUrl":"https://doi.org/10.1111/plb.70278","url":null,"abstract":"Strawberry micropropagation produces uniform, disease-free planting material. However, repeated in vitro subculturing can simplify the endophytic microbiome, reducing plant vigour and acclimatization success. This study investigated the impact of successive in vitro generations on the endomicrobiome of Fragaria × ananassa cv. San Andreas and determined if targeted biotization with beneficial rhizobacteria can enhance plant performance. Profiled endophytic bacterial and fungal communities were analysed in the mother plant and across three in vitro generations using amplicon sequencing of the 16S rRNA gene and ITS markers. Ten plant growth-promoting bacterial strains were tested in vitro. The most effective strains were combined into consortia, and their impact on growth, survival and phenolic and flavonoid accumulation was assessed under in vitro and ex vitro acclimatization conditions. Successive subcultures simplified the endophytic assemblage. The mother plant exhibited substantially higher bacterial alpha-diversity than in vitro generations, with richness and Shannon diversity declining significantly (P <0.001) across all subcultures. In vitro biotization with A. brasilense Ab-V5 and A. brasilense Ab-V6 increased total dry biomass from 190.63 mg in the control to 216.75 mg and 209.11 mg, respectively. Under ex vitro conditions, several inoculated treatments achieved 100% survival compared with 75% in the non-inoculated control. Prolonged in vitro maintenance reduces the complexity of the strawberry endomicrobiome by imposing a selective bottleneck. Targeted introduction of beneficial bacteria partially compensates by enhancing growth, survival and secondary metabolite accumulation, supporting microbiome-assisted strategies to improve tissue culture performance and ex vitro establishment.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148758619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drought-induced embolism shapes sex-specific responses by regulating hydraulic conductivity in Populus cathayana and Salix babylonica. 干旱诱导的栓塞通过调节白杨和巴比伦柳的水力传导来形成性别特异性反应。

IF 3.4 3区生物学

Plant Biology Pub Date : 2026-08-16 DOI: 10.1111/plb.70280

L Tan, X Zhang, F Chen, L Xia, S Zhang

{"title":"Drought-induced embolism shapes sex-specific responses by regulating hydraulic conductivity in Populus cathayana and Salix babylonica.","authors":"L Tan, X Zhang, F Chen, L Xia, S Zhang","doi":"10.1111/plb.70280","DOIUrl":"https://doi.org/10.1111/plb.70280","url":null,"abstract":"Drought is a key constraint on plant growth and development. Poplars and willows are dioecious plants, and sex-specific responses to drought differ across species. However, little is known about how xylem embolism drives sex-specific hydraulic failure. In this study, male and female Populus cathayana and Salix babylonica were used to investigate the role of embolism in sex-specific drought responses. Drought significantly reduced plant growth and photosynthetic capacity, increased membrane damage and reactive oxygen species accumulation, altered vessel structure and aggravated embolism. P. cathayana males maintained higher net photosynthetic rates than females, whereas S. babylonica females performed better than males under drought stress. P. cathayana males exhibited larger vessel area and diameter but lower density than females, while the opposite sex pattern was observed in S. babylonica. In terms of hydraulic function, male P. cathayana and female S. babylonica had lower embolism degrees, higher hydraulic conductivity and maintained more stable conductivity than their opposite sexes. Embolism degree was strongly correlated with both the standardized vessel structure index and the standardized photosynthetic index (R2 = 0.81 and 0.93 for P. cathayana; 0.78 and 0.91 for S. babylonica, respectively). Overall, male P. cathayana and female S. babylonica were more resistant to embolism, sustaining higher photosynthetic performance and growth. These findings suggest that drought alters xylem vessel structure, thereby inducing embolism and restricting growth, which forms the core mechanism underlying sex-specific drought responses in P. cathayana and S. babylonica.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148766142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Floral adaptation and modular trait evolution associated with a shift from bee to hummingbird pollination. 从蜜蜂传粉到蜂鸟传粉的转变与花的适应和模块化特征进化有关。

IF 3.4 3区生物学

Plant Biology Pub Date : 2026-08-06 DOI: 10.1111/plb.70277

P Juárez, K M Kay

{"title":"Floral adaptation and modular trait evolution associated with a shift from bee to hummingbird pollination.","authors":"P Juárez, K M Kay","doi":"10.1111/plb.70277","DOIUrl":"https://doi.org/10.1111/plb.70277","url":null,"abstract":"Pollination shifts result in floral divergence, yet how such shifts influence variation and correlation among floral traits is unclear. We examine whether a transition from bee to hummingbird pollination in sister species of Neotropical spiral gingers - Costus kuntzei, with ancestral bee pollination, and Costus wilsonii, with derived hummingbird pollination - drives phenotypic variation, functional integration and morphological modularity. Using field measurements and observations, we assess pollinator assemblages and pollination syndrome divergence by quantifying a suite of floral traits related to shape, size, colour, scent and nectar reward. We then test whether the shift from bee to hummingbird pollination has led to differences in floral phenotypic variation and integration, and we use geometric morphometric analyses to evaluate modularity hypotheses based on floral function and genetic or developmental constraints. We find that C. wilsonii exhibits changes in, and loss of, multiple floral traits, along with extreme specialization on a single hermit hummingbird species. In contrast, C. kuntzei is visited by a more species-rich pollinator assemblage of euglossine bees and a tabanid fly. Both plant species show similar patterns of floral variation, integration and modularity, with reduced floral variation and modularity patterns concentrated in traits involved in pollinator fit and pollen transfer. The findings suggest that phenotypic patterns reflect functional traits shaped by divergent pollinator-mediated adaptation. This floral adaptation has resulted in stable patterns of trait variation and correlation, consistent with selection for efficient pollination and pollinator fit in both species.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148682803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Saturating mutagenesis on the Ser118 residue responsible for the spectral tuning mechanism of the orange/green-reversible cyanobacteriochrome AM1_1499g1. 橙/绿可逆蓝藻色素AM1_1499g1光谱调谐机制Ser118残基的饱和诱变

IF 3.4 3区生物学

Plant Biology Pub Date : 2026-08-06 DOI: 10.1111/plb.70275

A Yoneda, T Suzuki, R Narikawa

{"title":"Saturating mutagenesis on the Ser118 residue responsible for the spectral tuning mechanism of the orange/green-reversible cyanobacteriochrome AM1_1499g1.","authors":"A Yoneda, T Suzuki, R Narikawa","doi":"10.1111/plb.70275","DOIUrl":"https://doi.org/10.1111/plb.70275","url":null,"abstract":"Cyanobacteriochromes (CBCRs) are widely distributed photoreceptors in cyanobacteria, crucial for regulating light-responsive phenomena. CBCR GAF domains form a stable covalent bond with a linear tetrapyrrole chromophore via the canonical Cys residue and exhibit reversible photoconversion. The DXCF (Asp-Xxx-Cys-Phe) -type CBCRs, a dominant subgroup across various clades, are characterized by a highly conserved second Cys residue involved in both phycocyanobilin (PCB)-to-phycoviolobilin (PVB) isomerization and reversible ligation to the chromophore. The AM1_1499g1 GAF domain, categorized as a DXCF-type CBCR, is unique due to the evolutionary loss of this second Cys residue, which is substituted by Ser118. This domain exhibits an orange/green-reversible photocycle alongside thermochromism in its photoproduct state, suggesting a novel spectral tuning mechanism to be elucidated. To elucidate the molecular basis of the spectral tuning mechanism in AM1_1499g1, we performed saturation mutagenesis at the Ser118 position and comprehensively performed spectral analyses of the resulting S118X variants. Spectral analyses classified the S118X mutants into five distinct functional groups based on PCB-to-PVB isomerization activity, thermochromism and photocycle property. These results lead us to propose that the side chain property at Ser118, located near the chromophore centre, affects the chromophore conformation, especially the A-ring conformation, thereby controlling the domain's spectral properties. Framed within the evolutionary transition of AM1_1499g1, our findings demonstrate that single-site mutagenesis at Ser118 is sufficient to recapitulate a broad range of spectral properties. This variation functionally mimics the natural evolutionary divergence of XRG (extended red/green)-type CBCR domains, highlighting the exceptional molecular plasticity of this photoreceptor family.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148682798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global research trends and hotspots of plant physiologically active substances: quantitative visualization analysis based on CiteSpace. 全球植物生理活性物质研究趋势与热点：基于CiteSpace的定量可视化分析

IF 3.4 3区生物学

Plant Biology Pub Date : 2026-08-01 Epub Date: 2026-06-13 DOI: 10.1111/plb.70219

P Chu, Y He

{"title":"Global research trends and hotspots of plant physiologically active substances: quantitative visualization analysis based on CiteSpace.","authors":"P Chu, Y He","doi":"10.1111/plb.70219","DOIUrl":"10.1111/plb.70219","url":null,"abstract":"Plant-derived physiologically active substances represent a rapidly evolving interdisciplinary research field with significant implications for human health, nutrition and industry. Utilizing CiteSpace-based bibliometric and visualization analyses, this study systematically reviewed 8,096 documents from the Web of Science Core Collection (WoSCC-SCIE) published between 2000 and 2024. The results highlighted exponential growth in research outputs, emphasizing a notable shift from basic compound isolation and antioxidant assays towards advanced mechanistic studies, functional food applications and innovative nano-delivery systems. The thematic evolution illustrated a clear transition across distinct research stages, driven by technological advances in metabolomics and synthetic biology, rising consumer demands for natural products and stringent regulatory frameworks. China, India, and the United States emerged as global research leaders, with expanding international collaboration networks facilitating knowledge dissemination. Core institutions, including the Chinese Academy of Sciences and India's Council of Scientific and Industrial Research, significantly influenced global research dynamics through extensive interdisciplinary cooperation. Emerging hotspots identified through keyword burst analysis encompassed plant immunity modulation, gut microbiota interactions, metabolic disease interventions and molecular dynamics simulations. Future research priorities highlighted include integrative multi-omics methodologies, climate-resilient phytochemical production, synthetic biology-driven compound biosynthesis and enhanced clinical translation frameworks. Overall, this bibliometric investigation provides an essential roadmap for guiding future research strategies, promoting interdisciplinary collaborations and facilitating sustainable industrial exploitation of plant-derived bioactive substances.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":"1743-1760"},"PeriodicalIF":3.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13358663/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148248488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical treatment rescues reduced growth of the autoimmune mutant chs3-2D without compromising its immune responses. 化学治疗可以在不影响其免疫反应的情况下挽救自身免疫突变体chs3-2D的生长。

IF 3.4 3区生物学

Plant Biology Pub Date : 2026-08-01 Epub Date: 2026-03-22 DOI: 10.1111/plb.70211

M Keijzer, M Liebers, J Seufer, L Voll, C Meier, S Hoth

{"title":"Chemical treatment rescues reduced growth of the autoimmune mutant chs3-2D without compromising its immune responses.","authors":"M Keijzer, M Liebers, J Seufer, L Voll, C Meier, S Hoth","doi":"10.1111/plb.70211","DOIUrl":"10.1111/plb.70211","url":null,"abstract":"Plant development in many species including Arabidopsis relies on the accurate balance between growth and defence. A high degree of plant immunity is often accompanied by reduced growth. On the one hand, this growth-defence trade-off depends on the redistribution of metabolic power to either growth or defence. On the other hand, its regulation requires distinct molecular signalling pathways. Reduced growth of the Arabidopsis autoimmune mutant chs3-2D, which shows strong resistance to pathogens, has been rescued by the addition of the low molecular weight compound Ro 8-4304. The application of Ro 8-4304, however, also resulted in a decrease of immune responses to wild-type levels. We applied chemical modification of Ro 8-4304 to identify a derivative that rescues growth of the autoimmune mutant chs3-2D without compromising it immune capacity. In a screening of Ro 8-4304 derivatives, removal of a fluorine atom resulted in the derivative Ro-A03 that rescued growth, but did not affect the increased immune gene expression in chs3-2D. In the presence of Ro-A03 chs3-2D seedlings showed wild-type-like growth, but were more resistant to bacterial pathogens than wild-type seedlings, in line with salicylic acid levels that were higher than in the absence of Ro-A03. We generated a compound by chemical modification that can efficiently rescue growth without decreasing the immune response of the chs3-2D autoimmune mutant.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":"1686-1697"},"PeriodicalIF":3.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13358709/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147497132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ibero-Balearic plant disjunctions: genomic support for rapid and recurrent long-distance colonizations of the endangered Diplotaxis ibicensis (Brassicaceae) despite no dispersal syndromes. 伊比利亚-巴利阿里群岛植物的分离：尽管没有传播综合征，但濒危的ibicensis（芸苔科）快速和反复的长距离殖民的基因组支持。

IF 3.4 3区生物学

Plant Biology Pub Date : 2026-08-01 Epub Date: 2026-06-15 DOI: 10.1111/plb.70227

L Bezares, M Fernández-Mazuecos, E Cano, J Borràs, J Cerca, J Cursach, P Vargas

{"title":"Ibero-Balearic plant disjunctions: genomic support for rapid and recurrent long-distance colonizations of the endangered Diplotaxis ibicensis (Brassicaceae) despite no dispersal syndromes.","authors":"L Bezares, M Fernández-Mazuecos, E Cano, J Borràs, J Cerca, J Cursach, P Vargas","doi":"10.1111/plb.70227","DOIUrl":"10.1111/plb.70227","url":null,"abstract":"Diplotaxis ibicensis is a western Mediterranean plant endemic to the Balearic Islands and a small area of the eastern Iberian Peninsula. This study investigates the biogeographical relationships among 16 populations across its entire distribution using two complementary approaches: (i) an ITS-based phylogenetic analysis to reconstruct Diplotaxis species relationships and divergence times, and (ii) phylogenomic and population genomic analyses of D. ibicensis via genotyping-by-sequencing (GBS) to assess divergence among populations. The ITS phylogeny supports D. ibicensis as a monophyletic group closely related to the western Mediterranean species D. siettiana, D. brevisiliqua and D. ilorcitana. A time-calibrated tree estimates the most recent common ancestor of these four species in the Pliocene-Pleistocene (3.68-0.77 Ma), well after the Messinian Salinity Crisis (5.96-5.3 Ma). GBS analyses identified six well-supported clades of D. ibicensis individuals, four of them restricted to single islands and the mainland, while two clades contained populations from different islands. These results suggest that rapid long-distance dispersal (LDD) events occurred across permanent marine barriers, followed by secondary colonizations among distant islands, but strong isolation in others. The highest genetic diversity (π) occurs on the island of Ibiza, while the greatest genetic differentiation (FST) is found between mainland and Cabrera island populations. Recurrent LDD events contrast with the absence of specialized LDD traits in pollen, fruits and seeds. A review of studies of Ibero-Balearic species reveals similar patterns of LDD across the so-called Ibiza Channel. Conservation strategies are proposed based on patterns of genetic diversity and differentiation.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":"1560-1572"},"PeriodicalIF":3.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13358666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148248501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PgUGT84K2, a Panax ginseng glycosyltransferase, catalyses IBA glycosylation and enhances abiotic stress tolerance in transgenic Arabidopsis. PgUGT84K2是一种人参糖基转移酶，可催化IBA糖基化，提高转基因拟南芥对非生物胁迫的耐受性。

IF 3.4 3区生物学

Plant Biology Pub Date : 2026-08-01 Epub Date: 2026-03-25 DOI: 10.1111/plb.70206

F Chang, S Ma, Y Yu, Z Wang, J He, X Gong, G Zhang

{"title":"PgUGT84K2, a Panax ginseng glycosyltransferase, catalyses IBA glycosylation and enhances abiotic stress tolerance in transgenic Arabidopsis.","authors":"F Chang, S Ma, Y Yu, Z Wang, J He, X Gong, G Zhang","doi":"10.1111/plb.70206","DOIUrl":"10.1111/plb.70206","url":null,"abstract":"Glycosylation plays a crucial role in modulating auxin homeostasis through the formation of auxin glycoside conjugates. However, the involved mechanisms and relevant physiological significances are largely unknown or poorly understood. PgUGT84K2 enzyme was purified and the enzyme activity was identified by HPLC and LC/MS analyses. We heterologously overexpressed PgUGT84K2 in Arabidopsis thaliana and analysed glycosyltransferase activity and glycosylated metabolites IBA-Glc in transgenic plants. Drought and salt stress assays of the transgenic plants were conducted and the expression of related genes was analysed by quantitative RT-PCR. Panax ginseng glycosyltransferase PgUGT84K2 was at the first time identified to be an IBA glycosyltransferase. Assessment of enzyme activity and IBA conjugates in transgenic plants ectopically expressing PgUGT84K2 indicated that the PgUGT84K2 catalytic specificity was maintained in planta. Heterologous overexpression of PgUGT84K2 in Arabidopsis thaliana led to significantly enhanced tolerance to drought and salt stresses, with improved germination rates, seedling greening rates and seedling survival rates than the wild type. Overexpression of PgUGT84K2 enhanced ROS scavenging in transgenic plants and quantitative RT-PCR analysis revealed the up-regulation of key antioxidant enzyme genes SOD1, SOD3, CAT2, CAT3 and ABA biosynthesis genes AAO1, AAO3, along with down-regulation of ABA signalling repressors EM1 and EM6. Our results suggest that PgUGT84K2, an IBA glycosyltransferase, might enhance stress tolerance through modulating ABA signalling and enhancing ROS scavenging via the enzymatic antioxidant system, and provide a potential strategy to breed drought and salt resistant P. ginseng.","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":" ","pages":"1669-1685"},"PeriodicalIF":3.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13358660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147508347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0