Haya: The Saudi Journal of Life Sciences最新文献

{"title":"Recent Advances in Bacterial and Viral Genomics for Cancer Therapy","authors":"Mamoon Tajamal, Ambreen Qasim, Qasim Javed, Marium Majeed, S. Imtiaz, Mehwish Mehwish, Taiyyibah Basharat, S. Yousaf, R. Javed","doi":"10.36348/sjls.2020.v07i10.004","DOIUrl":"https://doi.org/10.36348/sjls.2020.v07i10.004","url":null,"abstract":"Bacterial and viral therapies have gained enough success and attention for biological cancer treatments. There is a need to protect the living tissues of the body that are helpful for activating the immune responses. Many failures are associated with the conservational therapies that render them for the current era of medical sciences. Viral and bacterial genomes have been used for targeting the initial basis of metastasis and inhibiting the growth of cancerous cells. S. pyogenes OK-432 strain showed action against cancerous cells with intact bindings to the proliferative tissues. Phenazine 1-carboxylic acids have anticancer nature and pose a high level of cytotoxicity against the. Salmonella strain (KST0650) showed maximum potential against CT26 cancer cells. Oncolytic biologically and virologically group of the controlled viruses that have been used for tumor treatment. Some critical cancers under medications such as hormone therapy- induced helpful for prolonged life span. Pro-active drugs and combinations of probiotics also prevent the bacteria from synthesizing the protective layer of peptidoglycan.","PeriodicalId":219819,"journal":{"name":"Haya: The Saudi Journal of Life Sciences","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126034491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Earnings Management, Conceptual Framework and Literature Review: Saudi Arabia as a Case Study","authors":"Yasmin Abdulaziz Al-Harby, Bilel Jarraya","doi":"10.36348/sjls.2020.v07i10.003","DOIUrl":"https://doi.org/10.36348/sjls.2020.v07i10.003","url":null,"abstract":"This article provides the conceptual framework for earnings management, which begins by presenting the concept of earnings management, then the actions, motives, techniques, measurement, and then the consequences of this practice. This article also presented studies that dealt with earnings management in the Saudi context. This is because the Kingdom of Saudi Arabia has recently witnessed a clear development in companies and changes in accounting systems which in turn provides an opportunity for more research on earnings management in Saudi Arabia. Several studies provide evidence that EM occurs in Saudi companies, and there are many motivations to practice earnings management in Saudi companies.","PeriodicalId":219819,"journal":{"name":"Haya: The Saudi Journal of Life Sciences","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114461678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bitterleaf (Venonia amygdalina) and Water leaf (Talinum triangulare) in Pullets Nutrition: Effects on Blood Vitamins and Minerals","authors":"F. Igene","doi":"10.36348/sjls.2020.v07i10.002","DOIUrl":"https://doi.org/10.36348/sjls.2020.v07i10.002","url":null,"abstract":"The effect of bitter leaf (Venonia amygdalina) and water leaf (Talinum triangulare) extracts on blood vitamins and minerals composition of pullet birds were investigated. A total of 456 day-old Isa brown pullet chicks were allotted to four dietary treatments in six replicates in a completely randomized design experiment. Aqueous extracts of bitter leaf and water leaf were used as test dietary treatments for the birds. In treatment 1, a commercial multivitamin (vitalyte) was given in water (1g/L) to the birds to serve as the control. In treatments 2 and 3 aqueous extracts of bitter leaf and waterleaf were given at 100ml/L of water, while in treatment 4 mixed bitter leaf and water leaf aqueous extract (50% each in composition) was given. On the last day of the feeding trial, blood samples were collected from the birds for vitamins and minerals analysis. The observed results showed that dietary treatments significantly affected the blood serum parameters. Vitamin A was significantly higher (P < 0.05) in the vitalyte birds. The values ranged from 0.27iu in the bitter leaf birds to 0.37iu in the vitalyte. Vitamin C was more abundant (1.84iu) in the bitter leaf treated pullets and was significantly reduced (1.21iu) in the vitalyte. Vitamin D was significantly (P < 0.05) higher (0.35 iu) in the water leaf as well as vitamin E (10.14iu) while they were highly reduced considerably in the vitalyte administered birds, 0.16 and 4.83iu respectively. On mineral composition, while bitter leaf was the highest Ca (14.24mg/dl) source, K (5.46 mmol/L) and Mg (2.78mg/dl) were highly increased in the water leaf administered birds. Pullets on the control vitalyte had higher Na (132.75 mmol/L) and Fe (1.42g/dl). It can however be concluded that bitter leaf and water leaf as used in this study are potential sources of some vitamins and minerals in pullet chick nutrition.","PeriodicalId":219819,"journal":{"name":"Haya: The Saudi Journal of Life Sciences","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125950751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Bush Buck (Gongronema latifolia) as a Dietary Supplement on Haematological and Serum Biochemical Indices of Broiler Chickens","authors":"F. Igene, Iwegbu A","doi":"10.36348/sjls.2020.v07i10.001","DOIUrl":"https://doi.org/10.36348/sjls.2020.v07i10.001","url":null,"abstract":"The high cost of conventional feed ingredients for livestock and competition between man and his animals, necessitate the need for alternative and under-utilized plant materials in poultry nutrition. However, in doing so, it is important to ensure that such plant materials are not deleterious to the animals. This research therefore examines the effect of bush buck (Gongronema latifolia) leaf extracts as dietary supplements on haematological and serum biochemical parameters of broiler chickens. Fresh leaves (6kg) of bush buck (Gongronema latifolia) were after rinsing with de-ionized water air-dried for ten (10) days under room temperature and were ground in a hammer mill to pass through 0.5mm sieve. It was thereafter dissolved in de-ionized warm water (40oC) at 1kg per 5 litres of water and was left to settle for 1 hour before decanting the supernatant. One hundred and ninety-two (192) broiler chicks, after brooding for two weeks were divided into six (6) dietary treatment groups of thirty two (32) birds each. Birds on dietary treatment 1, had no vitamin/mineral supplement in water, while dietary treatment 2 birds had supplemental commercial vitamin/mineral premix (vitalyte extra). Dietary treatments 3, 4, 5 and 6 had Gongronema latifolia leaf warm water extract in the following ratios; 25, 50, 75, and 100ml/litre of water respectively. The vitalyte extra was administered (5g/l) once a week while the birds had access to feed and water/GLLE ad libitum. On the last day of the experiment, blood samples were collected from the birds for haematological analysis. All the assayed parameters such as packed cell volume (PCV), white blood cell (WBC), red blood cell (RBC), heterophil (H), lymphocyte (L), monocyte (M), eosinophil (E), basophil (B), haemoglobin (Hb), mean corpuscular volume (MCV), mean corpuscular haemoglobin concentration (MCHC) and mean corpuscular haemoglobin (MCH) were significantly (P<0.05) influenced by the dietary treatments. The analyzed serum biochemical indices such as total protein, albumin, globulin, creatinine, uric acid, aspartate aminotransferase, alanine aminotransferase, sodium and potassium were generally better in the 25ml/l GLLE. Glucose was significantly reduced in the vitalyte treatment while total cholesterol, high and low density lipoprotein were least in the 50ml/l GLLE treatment. In conclusion, the results above revealed that the Gongronema latifolia leaf warm (40oC) water extract of 50ml/l was safer and supported better health and wellbeing of the broiler chickens. This shows that Gongronema latifolia leaf warm (40oC) water extract could be used to wholly replace the conventional vitamins and minerals (vitalyte extra) in broiler diets without any adverse effect on growth and health performance of the birds.","PeriodicalId":219819,"journal":{"name":"Haya: The Saudi Journal of Life Sciences","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122380583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering Beneficial Health Promoting Properties of Lactic Acid Bacteria Isolated From Infant Gut Microbiome","authors":"K. Raghavan, A. Savithri, Honey Chandran C, Sabu K. Thomas","doi":"10.36348/sjls.2022.v07i09.003","DOIUrl":"https://doi.org/10.36348/sjls.2022.v07i09.003","url":null,"abstract":"Aim and Objective: To evaluate the probiotic potenties along with safety of probiotics within infant faecal origin. Methods and Results: The present study isolates lactic acid bacteria from infant feces were characterized and identified by 16S rRNA sequencing. On evaluation of in vitro probiotic capability, the test isolates exhibited in vitro probiotic potency, antagonistic property and better cell surface properties. The Bile salt hydrolase activity, cholesterol assimilation, antioxidant activity and angiotensin converting enzyme inhibitory action of the test isolates proves their health conferring potency. Standard protocols were used for the estimation of lymphocyte proliferation rate, phagocytosis rate, cytokine response and faecal enzyme levels. Animal experiment using different doses of L. plantarum (MBTU-S1B) substantiated its immunotolerance against inhibitory effects of supraoptimal concentration of both T and B cell mitogens with significant reduction on β-Glucosidase and Nitroreductase activity with p< 0.05, while contrasting effect was noticed for β-Glucuronidase. Findings of the in vivo study proved higher dose of probiotic would not provide any additional benefit on host body. Conclusions: The test isolates possesed in vitro probiotic potency and the in vivo study with MBTU- S1B proved their applicability in nutraceutical and pharmaceutical industries. Significance and Impact of Study: To conflict disease outbreaks by improving general immunity via probiotics.","PeriodicalId":219819,"journal":{"name":"Haya: The Saudi Journal of Life Sciences","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130642903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Lactic Acid Bacteria on the Quality Attributes of Tea-Like Product from Blends of Drumstick (Moringa oleifera) Leaves and Ginger (Zingiber officinale)","authors":"Okorieuku C. B. N, Oaoye, O. A, Joel. N, S. Y, A. Y, M. A.","doi":"10.36348/sjls.2022.v07i09.002","DOIUrl":"https://doi.org/10.36348/sjls.2022.v07i09.002","url":null,"abstract":"Moringa oleifera leaves and Ginger crops still remain an underutilized commodity because of limited information on its wider use. Moringa oleifera is very rich in nutrition but has undesirable taste and flavor. However, addition of ginger takes the edge off the bitter spinachy taste of Moringa oleifera leaves. Lactic acid bacteria (LAB) are important microbes that have long been used in both traditional and modern industrial food fermentation. Lactic acid bacteria can be used for production of food due to their ability to coax microorganisms into producing In the process of food fermentation; microorganisms produce enzymes to break down complex compounds to simple bio-molecules for several biological activities such as proteinase, amylase, mannase, cellulase, and catalase. The aim of this research work is to determine the effect of Lactic acid bacteria (LAB) fermentation on the quality attributes of tea-like beverage produced from blends of Moringa oleifera leaves and Ginger(Zingiber officinale). The results showed that The phenotypic and biochemical characteristic of Blends of Drumstick leaves (Moringa oleifera) and Ginger (Zingiber officinale) reveals that the isolates were presumptively categorized to belong to the genus Lactobacillus, the isolates are gram positive, cocci, rod shaped, either, short rod or long rods, non-sporulating, ferments carbohydrate, non-motile and catalase negative. Technological properties of isolates are the properties that indicate the quality of the isolate to be used as a starter culture in the course of fermentation. A total of 35 colonies were isolated from the blend samples. Of them, 3 colonies were identified as Lactobacillus species, through observation of phenotypic characters, and identification, It was found that L. plantarum had tolerance under acid conditions, as its viable cell count remained at pH 2.0 and under 1.0 % bile salt condition, after 72 h incubation time. The proximate and energy content of fermented beverage produced from the blends shows that percent Dry matter, Moisture content, Crude protein, Ether extract , Crude fibre, Ash, carbohydrate and energy value were ranged from 10.00- 30.00, 4.0-5.0, 35.00 -71.00, 3.0-5.0, 11.00 - 18.00,3.0-10,7.0-15 and 74Kcal - 396Kcal respectively. The study concludes that The phenotypic and biochemical characteristic of blends of drum stick leaves (Moringa oleifera) and Ginger (Zingiber officinale) reveals that the isolates were to belong to the genus Lactobacillus. Also, Many of the LAB metabolites such as low molecular weight acids, alcohols, carbon dioxide, diacetyl and hydrogen peroxides have broad spectrum antimicrobial activity against other species of bacteria. The proximate and energy value of the beverage plays a crucial role in assessing its nutritional significance (P<0.05). Consequently, the fermentation of these blends with LAB species might provide a means of retaining more of the nutrients that are trapped in the matrix of the cells of these p","PeriodicalId":219819,"journal":{"name":"Haya: The Saudi Journal of Life Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130911047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Insights for Improving Agriculture Soils through Nanotechnology","authors":"N. Azam, Muhammad Waqas Khan, Saba Sardar, Iqra Yousaf, Alina Zahid, Anam Ismail, Syeda Summiya, Ramsha Mushtaq, Muhammad Sheeraz Javed","doi":"10.36348/sjls.2022.v07i09.001","DOIUrl":"https://doi.org/10.36348/sjls.2022.v07i09.001","url":null,"abstract":"Different efforts have been made especially in the fields of nanotechnology that employed various combinations of nanoparticles to overcome the stresses occurred in the agricultural lands. Nanoparticles that potentially designed through nanotechnology applied to the soil in the form nanofertilizers that increased the yield also good impact on soil composition. Application of nano-SiO2 improves also seed germination throiugh rooting applications, chlorophyll content with proline accumulation. Soil composition also improved the overall fruits and vegetables varieties in such a way that nanoparticles makes intact combinations with inner layers of soil. Biological applications of iron sulfate (FeSO4) in the form of foliar spray also showed excellent resistance to the salinity stress tolerance in the case of sunflower cultivars. Nano zeolite; another type of newly modified materials efficient for long term delivery of nutrients and minerals in soil composition and mineral balance ensures for plant growth. Nanopesticides can be used for efficient crop protection also promote plant stress tolerance and soil enhancement. Nano-Si that improves the various other stresses in plants species such as nickle and chromium. Nanotechnology has several advantages over other technologies due to cost efficient and simple to use and imply on more effective treatment and remediation methods.","PeriodicalId":219819,"journal":{"name":"Haya: The Saudi Journal of Life Sciences","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121244758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variations In The PEFR With Various Factors With Respect To Age, Body Mass Index, Blood Pressure And Heart Rate Among Males And Females Patients","authors":"Md. Soroar Hossain, Sarwat Jahan Zubyra, Mohammad Zakir Hossain, Tridip Kanti Barman, Md. Towfiqul Islam, Sk. Abdul Fattah","doi":"10.36348/sjls.2022.v07i08.002","DOIUrl":"https://doi.org/10.36348/sjls.2022.v07i08.002","url":null,"abstract":"Introduction: PEFR is considered as the simplest index of pulmonary function. Ventilatory function tests like PEFR provide a better understanding of the changes in the lungs from a diagnostic viewpoint. It is effort dependent and reflects mainly the calibre of the bronchi and larger bronchioles, which are subjected to reflex bronchoconstriction. Objective: To study the variations in the PEFR with various factors with respect to age, body mass index, blood pressure and heart rate among males and females patients. Methods: This was a cross-sectional study conducted at Department of Medicine, Shaheed Tajuddin Ahmad Medical College Hospital, Gazipur, Bangladesh from January to December 2020 among young male and female subjects. The subjects with no history of cardio, availability & capacity to cooperate adequately, non- smokers; and no history of any drug intake which could affect the lung functioning were included in the study. PEFR was measured with the subject comfortably seated during the same time of the day for all subjects. It was determined in litres/minute with the help of “The Peak”, Individualised Peak flow meter from Multispiro Inc. Results: Out of the total subjects 121 subjects, 45.4% were ≤18 years followed by 19-20 (42.1%) and >20 (12.3%). There were 37.1% males and 62.8% females. PEFR was found to be significantly (p=0.0001) higher among males compared to females in all the age groups. PEFR increased with increasing age in both male and female subjects. PEFR was found to be significantly (p<0.0) higher among males compared to females in the entire body mass index (BMI) category. PEFR increased with increasing BMI in both male and female subjects. Conclusion: PEFR is higher among male than female subject. Also, PEFR was higher in males than females in all the age groups and BMI status. The effects of various factors like age, height, weight, BSA and BMI on the PEFR values have also been discussed in this study. PEFR increases with increase in age and BMI in both male and female subjects.","PeriodicalId":219819,"journal":{"name":"Haya: The Saudi Journal of Life Sciences","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126668287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Concentration of Heavy Metals in the Sediments of the River Nun Estuary, Around Akassa, Niger Delta, Nigeria","authors":"G. H., A. J","doi":"10.36348/sjls.2022.v07i08.001","DOIUrl":"https://doi.org/10.36348/sjls.2022.v07i08.001","url":null,"abstract":"Levels of the heavy metals lead (Pb), cadmium (Cd), nickel (Ni), zinc (Zn), and manganese (Mn) were analyzed in the sediments of the River Nun estuary. This was done in order gauge the impact of anthropogenic inputs such as pesticides, fertilizers, oil spillage and other industrial and anthropogenic wastes disposal, especially at the coastal parts of the region on bottom sediments. Three sampling Stations were chosen for the purpose of this research. Station 1 (Buo-Ama Creek) was established close to the entrance of Buo-Ama creek, in the Nun River estuary. Station 2 (Erewei- Kongho) was established along one of the tributaries of the Nun River estuary around Erewei- Kongho. Station 3 was established in Ogbokiri. Sediment samples were collected with the aid of a soil auger and a galvanized metal core cylinder from the three (3) sampling stations along a transect running from the low intertidal level (LIL), through the mid intertidal level (MIL) to the high intertidal level (HIL). Sediment samples were analysed in the laboratory using the method of Atomic Absorption Spectrophotometer (AAS). Means and standard deviations were calculated for all the measured parameters. A one-way ANOVA was employed at the 95% confidence limit to test for differences across stations in the heavy metals. Turkey HSD post HOC test was also employed to separate means and identify where variability and similarities exist. This was aided by the SPSS 20.0 statistical tool kit. The result shows that Pb has the highest concentration, followed by Zn, Ni, Cd, and Mn respectively. The lowest values of all metals were observed at station 2, while the highest concentrations of metals were recorded at sampling stations 1. The results show that the value of Pb was highest in station 1(1.347±0.001), followed by Station 3 (1.24±0.001). Station 2 had the least mean value of Pb (0.884±0.001). Cd showed similar patterns across the three sampling stations 1, 2 and 3, with the values 0.068±0.002, 0.046±0.001, and 0.054±0.002 respectively. Ni also presented slight difference across the three sampling stations. Concentration of Zn were higher in station 1 and 2 (1.265±0.002 and 1.038±0.002), than in station 3 (0.725±0.001). All metal concentration was however lower than the international permissible limited. The concentrations of Mn were also observed to present a similar pattern across the sampling stations. There is a significant difference (P<0.05) in all heavy metal parameters across all stations. Heavy Metal Concentrations were found to decrease with increasing distance from the river mouth, suggesting that anthropogenic inputs, related to agricultural and domestic discharge into the river, are the major sources of heavy metals in the river sediments. Land based activities therefore have a profound effect on aquatic ecosystem stability and health.","PeriodicalId":219819,"journal":{"name":"Haya: The Saudi Journal of Life Sciences","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125494188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulation of Wound Healing Transdermal Patch from Tubers Extract of Momordica Cymbalaria and its In-vitro Evaluation","authors":"P. Saundharya, Jerrine Joseph, G. Rajalakshmi, Mary Shamya","doi":"10.36348/sjls.2022.v07i07.004","DOIUrl":"https://doi.org/10.36348/sjls.2022.v07i07.004","url":null,"abstract":"Developing wound infection in type Ⅰ or Ⅱ diabetic people had risk complications like gangrene. Although there are enough antibiotic ointments or creams, still it needs physician dressing and continuous monitoring of wound. So, to make it simple and more effective, a combination of transdermal drug delivery route with herbal usage contribute in controlled release of medication with minimum undesirable side effects. And also help to treat wound infections by self-medication. This work is focused, to formulate wound healing transdermal patch for diabetics with Momordica cymbalaria tuber extract. A sequence of techniques was performed to analyse antioxidant content then Starch assay was performed In vitro to evaluate its healing efficacy, determine cytotoxicity using zebra fish embryos and artemia salina using hexane extract of tuber. Optimized combination is used to prepare Patch by solvent evaporation method then evaluated the patch by organoleptic and physico-chemical properties. The result proofs, the tuber extract had Strong Antioxidant activity whereas weak cytotoxicity showed in two concentration (25 and 50µg/ml) towards brine shrimp lethality assay and fish embryo toxicity. High migration rate found in 25µg/ml and 50µg/ml in scratch assay using Human Epidermal Keratinocytes. Evaluated the patch for its organoleptic and physico-chemical properties, it shows good result. As a final observation of the study, bio compounds present in tuber extract proved to enhance wound healing and could pave way as alternate to the synthetic wound healing patches owing to its ecofriendly, economical and herbal nature be natural replacement to hasten wound healing especially in diabetic patients.","PeriodicalId":219819,"journal":{"name":"Haya: The Saudi Journal of Life Sciences","volume":"110 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126762991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}