Sijing Chang, Gaosen Zhang, Ximing Chen, Haozhi Long, Yilin Wang, Tuo Chen, Guangxiu Liu
{"title":"The complete genome sequence of the cold adapted crude-oil degrader: <i>Pedobacter steynii</i> DX4.","authors":"Sijing Chang, Gaosen Zhang, Ximing Chen, Haozhi Long, Yilin Wang, Tuo Chen, Guangxiu Liu","doi":"10.1186/s40793-017-0249-z","DOIUrl":"10.1186/s40793-017-0249-z","url":null,"abstract":"<p><p><i>Pedobacter steynii</i> DX4 was isolated from the soil of Tibetan Plateau and it can use crude oil as sole carbon and energy source at 15 °C. The genome of <i>Pedobacter steynii</i> DX4 has been sequenced and served as basis for analysis its metabolic mechanism. It is the first genome of crude oil degrading strain in <i>Pedobacter</i> genus. The 6.58 Mb genome has an average G + C content of 41.31% and encodes 5464 genes. In addition, annotation revealed that <i>Pedobacter steynii</i> DX4 has cold shock proteins, abundant response regulators for cell motility, and enzymes involved in energy conversion and fatty acid metabolism. The genomic characteristics could provide information for further study of oil-degrading microbes for recovery of crude oil polluted environment.</p>","PeriodicalId":21965,"journal":{"name":"Standards in Genomic Sciences","volume":"12 ","pages":"45"},"PeriodicalIF":0.0,"publicationDate":"2017-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5531107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35238961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Complete genome sequence of the sand-sediment actinobacterium <i>Nocardioides dokdonensis</i> FR1436<sup>T</sup>.","authors":"Min-Jung Kwak, Soon-Kyeong Kwon, Jihyun F Kim","doi":"10.1186/s40793-017-0257-z","DOIUrl":"https://doi.org/10.1186/s40793-017-0257-z","url":null,"abstract":"<p><p><i>Nocardioides dokdonensis</i>, belonging to the class <i>Actinobacteria</i>, was first isolated from sand sediment of a beach in Dokdo, Korea, in 2005. In this study, we determined the genome sequence of FR1436, the type strain of <i>N. dokdonensis</i>, and analyzed its gene contents. The genome sequence is the second complete one in the genus <i>Nocardioides</i> after that of <i>Nocardioides</i> sp. JS614. It is composed of a 4,376,707-bp chromosome with a G + C content of 72.26%. From the genome sequence, 4,104 CDSs, three rRNA operons, 51 tRNAs, and one tmRNA were predicted, and 71.38% of the genes were assigned putative functions. Through the sequence analysis, dozens of genes involved in steroid metabolism, especially its degradation, were detected. Most of the identified genes were located in large gene clusters, which showed high similarities with the gene clusters in <i>Pimelobacter simplex</i> VKM Ac-2033D. Genomic features of <i>N. dokdonensis</i> associated with steroid catabolism indicate that it could be used for research and application of steroids in science and industry.</p>","PeriodicalId":21965,"journal":{"name":"Standards in Genomic Sciences","volume":"12 ","pages":"44"},"PeriodicalIF":0.0,"publicationDate":"2017-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40793-017-0257-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35238960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Antonio E Serrano, Lorena V Escudero, Cinthya Tebes-Cayo, Mauricio Acosta, Olga Encalada, Sebastián Fernández-Moroso, Cecilia Demergasso
{"title":"First draft genome sequence of a strain from the genus <i>Fusibacter</i> isolated from Salar de Ascotán in Northern Chile.","authors":"Antonio E Serrano, Lorena V Escudero, Cinthya Tebes-Cayo, Mauricio Acosta, Olga Encalada, Sebastián Fernández-Moroso, Cecilia Demergasso","doi":"10.1186/s40793-017-0252-4","DOIUrl":"https://doi.org/10.1186/s40793-017-0252-4","url":null,"abstract":"<p><p><i>Fusibacter</i> sp<i>.</i> 3D3 (ATCC BAA-2418) is an arsenate-reducing halotolerant strain within the <i>Firmicutes</i> phylum, isolated from the Salar de Ascotán, a hypersaline salt flat in Northern Chile. This high-Andean closed basin is an athalassohaline environment located at the bottom of a tectonic basin surrounded by mountain range, including some active volcanoes. This landscape can be an advantageous system to explore the effect of salinity on microorganisms that mediate biogeochemical reactions. Since 2000, microbial reduction of arsenic has been evidenced in the system, and the phylogenetic analysis of the original community plus the culture enrichments has revealed the predominance of <i>Firmicutes</i> phylum. Here, we describe the first whole draft genome sequence of an arsenic-reducing strain belonging to the <i>Fusibacter</i> genus showing the highest 16S rRNA gene sequence similarity (98%) with <i>Fusibacter</i> sp. strain Vns02. The draft genome consists of 57 contigs with 5,111,250 bp and an average G + C content of 37.6%. Out of 4780 total genes predicted, 4700 genes code for proteins and 80 genes for RNAs. Insights from the genome sequence and some microbiological features of the strain 3D3 are available under Bioproject accession PRJDB4973 and Biosample SAMD00055724. The release of the genome sequence of this strain could contribute to the understanding of the arsenic biogeochemistry in extreme environments.</p>","PeriodicalId":21965,"journal":{"name":"Standards in Genomic Sciences","volume":"12 ","pages":"43"},"PeriodicalIF":0.0,"publicationDate":"2017-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40793-017-0252-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35238959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anish K Pandey, David W Cleary, Jay R Laver, Martin C J Maiden, Xavier Didelot, Andrew Gorringe, Robert C Read
{"title":"<i>Neisseria lactamica</i> Y92-1009 complete genome sequence.","authors":"Anish K Pandey, David W Cleary, Jay R Laver, Martin C J Maiden, Xavier Didelot, Andrew Gorringe, Robert C Read","doi":"10.1186/s40793-017-0250-6","DOIUrl":"https://doi.org/10.1186/s40793-017-0250-6","url":null,"abstract":"<p><p>We present the high quality, complete genome assembly of <i>Neisseria lactamica</i> Y92-1009 used to manufacture an outer membrane vesicle (OMV)-based vaccine, and a member of the <i>Neisseria</i> genus. The strain is available on request from the Public Health England Meningococcal Reference Unit. This Gram negative, dipplococcoid bacterium is an organism of worldwide clinical interest because human nasopharyngeal carriage is related inversely to the incidence of meningococcal disease, caused by <i>Neisseria meningitidis</i>. The organism sequenced was isolated during a school carriage survey in Northern Ireland in 1992 and has been the subject of a variety of laboratory and clinical studies. Four SMRT cells on a RSII machine by Pacific Biosystems were used to produce a complete, closed genome assembly. Sequence data were obtained for a total of 30,180,391 bases from 2621 reads and assembled using the HGAP algorithm. The assembly was corrected using short reads obtained from an Illumina HiSeq 2000instrument. This resulted in a 2,146,723 bp assembly with approximately 460 fold mean coverage depth and a GC ratio of 52.3%.</p>","PeriodicalId":21965,"journal":{"name":"Standards in Genomic Sciences","volume":"12 ","pages":"41"},"PeriodicalIF":0.0,"publicationDate":"2017-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40793-017-0250-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35238956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kurt C Showmaker, Mark A Arick, Chuan-Yu Hsu, Brigitte E Martin, Xiaoqiang Wang, Jiayuan Jia, Martin J Wubben, Robert L Nichols, Tom W Allen, Daniel G Peterson, Shi-En Lu
{"title":"The genome of the cotton bacterial blight pathogen <i>Xanthomonas citri</i> pv. <i>malvacearum</i> strain MSCT1.","authors":"Kurt C Showmaker, Mark A Arick, Chuan-Yu Hsu, Brigitte E Martin, Xiaoqiang Wang, Jiayuan Jia, Martin J Wubben, Robert L Nichols, Tom W Allen, Daniel G Peterson, Shi-En Lu","doi":"10.1186/s40793-017-0253-3","DOIUrl":"https://doi.org/10.1186/s40793-017-0253-3","url":null,"abstract":"<p><p><i>Xanthomonas citri</i> pv. <i>malvacearum</i> is a major pathogen of cotton, <i>Gossypium hirsutum</i> L.. In this study we report the complete genome of the <i>X. citri</i> pv. <i>malvacearum</i> strain MSCT1 assembled from long read DNA sequencing technology. The MSCT1 genome is the first <i>X. citri</i> pv. <i>malvacearum</i> genome with complete coding regions for <i>X. citri</i> pv. <i>malvacearum</i> transcriptional activator-like effectors. In addition functional and structural annotations are presented in this study that will provide a foundation for future pathogenesis studies with MSCT1.</p>","PeriodicalId":21965,"journal":{"name":"Standards in Genomic Sciences","volume":"12 ","pages":"42"},"PeriodicalIF":0.0,"publicationDate":"2017-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40793-017-0253-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35238958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Insights into <i>Cedecea neteri</i> strain M006 through complete genome sequence, a rare bacterium from aquatic environment.","authors":"Kok-Gan Chan, Wen-Si Tan","doi":"10.1186/s40793-017-0255-1","DOIUrl":"https://doi.org/10.1186/s40793-017-0255-1","url":null,"abstract":"<p><p><i>Cedecea neteri</i> M006 is a rare bacterium typically found as an environmental isolate from the tropical rainforest Sungai Tua waterfall (Gombak, Selangor, Malaysia). It is a Gram-reaction-negative, facultative anaerobic, bacillus. Here, we explore the features of <i>Cedecea neteri</i> M006, together with its genome sequence and annotation. The genome comprised 4,965,436 bp with 4447 protein-coding genes and 103 RNA genes.</p>","PeriodicalId":21965,"journal":{"name":"Standards in Genomic Sciences","volume":"12 ","pages":"40"},"PeriodicalIF":0.0,"publicationDate":"2017-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40793-017-0255-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35201329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Björn E Olsson, Ekaterina S Korsakova, Lyudmila N Anan'ina, Anna A Pyankova, Olga V Mavrodi, Elena G Plotnikova, Dmitri V Mavrodi
{"title":"Draft genome sequences of strains <i>Salinicola socius</i> SMB35<sup>T</sup>, <i>Salinicola</i> sp. MH3R3-1 and <i>Chromohalobacter</i> sp. SMB17 from the Verkhnekamsk potash mining region of Russia.","authors":"Björn E Olsson, Ekaterina S Korsakova, Lyudmila N Anan'ina, Anna A Pyankova, Olga V Mavrodi, Elena G Plotnikova, Dmitri V Mavrodi","doi":"10.1186/s40793-017-0251-5","DOIUrl":"https://doi.org/10.1186/s40793-017-0251-5","url":null,"abstract":"<p><p>Halomonads are moderately halophilic bacteria that are studied as models of prokaryotic osmoadaptation and sources of enzymes and chemicals for biotechnological applications. Despite the progress in understanding the diversity of these organisms, our ability to explain ecological, metabolic, and biochemical traits of halomonads at the genomic sequence level remains limited. This study addresses this gap by presenting draft genomes of <i>Salinicola socius</i> SMB35<sup>T</sup>, <i>Salinicola</i> sp. MH3R3-1 and <i>Chromohalobacter</i> sp. SMB17, which were isolated from potash mine tailings in the Verkhnekamsk salt deposit area of Russia. The analysis of these genomes confirmed the importance of ectoines and quaternary amines to the capacity of halomonads to tolerate osmotic stress and adapt to hypersaline environments. The study also revealed that <i>Chromohalobacter</i> and <i>Salinicola</i> share 75-90% of the predicted proteome, but also harbor a set of genus-specific genes, which in <i>Salinicola</i> amounted to approximately 0.5 Mbp. These genus-specific genome segments may contribute to the phenotypic diversity of the <i>Halomonadaceae</i> and the ability of these organisms to adapt to changing environmental conditions and colonize new ecological niches.</p>","PeriodicalId":21965,"journal":{"name":"Standards in Genomic Sciences","volume":"12 ","pages":"39"},"PeriodicalIF":0.0,"publicationDate":"2017-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40793-017-0251-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35188966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sujay Paul, Yvette Ludeña, Gretty K Villena, Fengan Yu, David H Sherman, Marcel Gutiérrez-Correa
{"title":"High-quality draft genome sequence of a biofilm forming lignocellulolytic <i>Aspergillus niger</i> strain ATCC 10864.","authors":"Sujay Paul, Yvette Ludeña, Gretty K Villena, Fengan Yu, David H Sherman, Marcel Gutiérrez-Correa","doi":"10.1186/s40793-017-0254-2","DOIUrl":"https://doi.org/10.1186/s40793-017-0254-2","url":null,"abstract":"<p><p>Filamentous fungus <i>Aspergillus niger</i> has high industrial value due to their lignocellulolytic enzyme activities and ATCC 10864 is one of the few type strains of <i>A. niger</i> which has a unique biofilm forming capability. Here we report the first draft genome sequence of <i>A. niger</i> ATCC 10864 strain. The genome of <i>A. niger</i> ATCC 10864 is 36,172,237 bp long and comprise of 310 scaffolds with 49.5% average GC content. A total of 10,804 protein-coding genes were predicted among which 10,761 genes were with putative functions. <i>A. niger</i> ATCC 10864 genome coded for 709 putative carbohydrate active enzyme families distributed in six functional categories and among them glycoside hydrolases (GHs) represent the most number of families (279). Genes that include <i>pepA</i>, <i>brlA</i>, <i>exgA</i>, <i>LaeA</i>, <i>rodA</i>, <i>GCN</i> have also been identified in this study, which may play a role in biofilm formation. This high-quality draft genome sequence will facilitate our understanding of the mechanisms behind fungal biofilm formation and higher lignocellulolytic enzyme production.</p>","PeriodicalId":21965,"journal":{"name":"Standards in Genomic Sciences","volume":"12 ","pages":"37"},"PeriodicalIF":0.0,"publicationDate":"2017-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40793-017-0254-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35184874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Byung Hee Chun, Se Hee Lee, Hye Hee Jeon, Dong-Woon Kim, Che Ok Jeon
{"title":"Complete genome sequence of <i>Leuconostoc suionicum</i> DSM 20241<sup>T</sup> provides insights into its functional and metabolic features.","authors":"Byung Hee Chun, Se Hee Lee, Hye Hee Jeon, Dong-Woon Kim, Che Ok Jeon","doi":"10.1186/s40793-017-0256-0","DOIUrl":"https://doi.org/10.1186/s40793-017-0256-0","url":null,"abstract":"<p><p>The genome of <i>Leuconostoc suionicum</i> DSM 20241<sup>T</sup> (=ATCC 9135<sup>T</sup> = LMG 8159<sup>T</sup> = NCIMB 6992<sup>T</sup>) was completely sequenced and its fermentative metabolic pathways were reconstructed to investigate the fermentative properties and metabolites of strain DSM 20241<sup>T</sup> during fermentation. The genome of <i>L. suionicum</i> DSM 20241<sup>T</sup> consists of a circular chromosome (2026.8 Kb) and a circular plasmid (21.9 Kb) with 37.58% G + C content, encoding 997 proteins, 12 rRNAs, and 72 tRNAs. Analysis of the metabolic pathways of <i>L. suionicum</i> DSM 20241<sup>T</sup> revealed that strain DSM 20241<sup>T</sup> performs heterolactic acid fermentation and can metabolize diverse organic compounds including glucose, fructose, galactose, cellobiose, mannose, sucrose, trehalose, arbutin, salcin, xylose, arabinose and ribose.</p>","PeriodicalId":21965,"journal":{"name":"Standards in Genomic Sciences","volume":"12 ","pages":"38"},"PeriodicalIF":0.0,"publicationDate":"2017-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40793-017-0256-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35184875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tsu Horng Moh, Nyok-Sean Lau, Go Furusawa, Al-Ashraf Abdullah Amirul
{"title":"Complete genome sequence of <i>Microbulbifer</i> sp. CCB-MM1, a halophile isolated from Matang Mangrove Forest, Malaysia.","authors":"Tsu Horng Moh, Nyok-Sean Lau, Go Furusawa, Al-Ashraf Abdullah Amirul","doi":"10.1186/s40793-017-0248-0","DOIUrl":"https://doi.org/10.1186/s40793-017-0248-0","url":null,"abstract":"<p><p><i>Microbulbifer</i> sp. CCB-MM1 is a halophile isolated from estuarine sediment of Matang Mangrove Forest, Malaysia. Based on 16S rRNA gene sequence analysis, strain CCB-MM1 is a potentially new species of genus <i>Microbulbifer</i>. Here we describe its features and present its complete genome sequence with annotation. The genome sequence is 3.86 Mb in size with GC content of 58.85%, harbouring 3313 protein coding genes and 92 RNA genes. A total of 71 genes associated with carbohydrate active enzymes were found using dbCAN. Ectoine biosynthetic genes, <i>ectABC</i> operon and <i>ask_ect</i> were detected using antiSMASH 3.0. Cell shape determination genes, <i>mreBCD</i> operon, <i>rodA</i> and <i>rodZ</i> were annotated, congruent with the rod-coccus cell cycle of the strain CCB-MM1. In addition, putative <i>mreBCD</i> operon regulatory gene, <i>bolA</i> was detected, which might be associated with the regulation of rod-coccus cell cycle observed from the strain.</p>","PeriodicalId":21965,"journal":{"name":"Standards in Genomic Sciences","volume":"12 ","pages":"36"},"PeriodicalIF":0.0,"publicationDate":"2017-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40793-017-0248-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35157177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}