South African Journal of Botany最新文献

{"title":"As nature's allies: Bee honey outperforms pomegranate and lemon juices in improving physio-biochemistry, antioxidants, RubisCO gene expression, and vase life of Calendula officinalis","authors":"Mostafa M. Rady ,&nbsp;Marwa S.M. Habib ,&nbsp;Ibrahim A.A. Mohamed ,&nbsp;Hesham F. Alharby ,&nbsp;Taia A.Abd El-Mageed ,&nbsp;El‑Sayed M. Desoky ,&nbsp;Alaa I.B. Abou-Sreea ,&nbsp;Esmat F. Ali","doi":"10.1016/j.sajb.2024.10.042","DOIUrl":"10.1016/j.sajb.2024.10.042","url":null,"abstract":"<div><div>Honey, pomegranate juice, and lemon juice are natural plant growth biostimulators that contain many bioactive substances that play essential roles in many plants’ physio-biochemical processes, and development. The objectives of this research were to explore how foliar spraying with a 5 % or 10 % diluted pomegranate juice solution (DPJS), a 5 % or 10 % diluted lemon juice solution (DLJS), or a 5 % or 10 % diluted honey solution (BHS), versus a control (distilled water; D-H₂O), positively influenced the growth, pharmaceutical, and ornamental qualities of calendula (<em>Calendula officinalis</em> L.) plants in both the 2021/2022 and 2022/2023 seasons. Foliar spraying with 5 %- or 10 %-DPJS, 5 %- or 10 %-DLJS, or 5 %- or 10 %-BHS significantly increased photosynthesis-related assessments (total chlorophyll and carotenoid contents, the maximum quantum efficiency of photosystem II (Fv/Fm), and net CO₂ assimilation), water use efficiency, RubisCO content and activity, <em>RubisCO</em> gene expression, and sugar fraction (glucose, sucrose, and starch contents). Also, osmoregulatory compounds (K⁺, total soluble sugars, and glycine betaine contents), antioxidant capacity (in terms of total flavonoid, anthocyanin, proline, ascorbate, and glutathione contents), nutrient and hormonal contents were significantly increased by foliar spraying of all the above-mentioned treatments. These positive findings were noticeably reflected in improved growth (fresh and dry plant weights), inflorescence traits (number of inflorescences on plant, fresh and dry petal weights, petal content of soluble sugars, ß-carotene, and total carotenoids), and longevity of inflorescence (vase life and water uptake by inflorescences), while electrolyte leakage and abscisic acid were noticeably decreased compared to foliar spraying with D-H₂O. The second-best treatment was 5 %-DPJS or 10 %-BHS, and they were preceded by 5 %-BHS as the best treatment, which the research results recommended applying in the field to obtain the best pharmaceutical and ornamental qualities of calendula.</div></div>","PeriodicalId":21919,"journal":{"name":"South African Journal of Botany","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142527683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic mechanism of Convolvulus pluricaulis against Alzheimer's disease: Network pharmacology and molecular dynamics approach","authors":"Lamya Ahmed Al-Keridis ,&nbsp;Laila Alshuraym ,&nbsp;Nawaf Alshammari ,&nbsp;Mohd Adnan ,&nbsp;Mitesh Patel","doi":"10.1016/j.sajb.2024.10.008","DOIUrl":"10.1016/j.sajb.2024.10.008","url":null,"abstract":"<div><div>Alzheimer disease (AD) is a type of dementia most prevalent in the elderly. A perennial herb, <em>Convolvulus pluricaulis</em> Choisy (<em>C. pluricaulis</em>) is used as a traditional folk medicine to treat AD and other neurodegenerative diseases, but the mechanism of action for its treatment has not been determined. This study attempts to clarify the mechanism of AD by investigating the phytochemical constituents of <em>C. pluricaulis</em> and their effect on it. In order to screen the active components of <em>C. pluricaulis</em>, values of oral bioavailability (OB) ≥ 30 % and Drug Like (DL) ≥ 0.18 was used. STRING and the Cytoscape platform have been used to construct an interactive network of targets and compounds. The network has been analyzed by Cytohubba, whereas GO functionality, KEGG pathways and metabolic pathway enrichment were performed using SRplot. The efficient targets were identified via Maximal Clique Centrality (MCC) and Degree using CytoHubba. The possible correlation between amyloid β-protein (Aβ), Tau pathology and identified efficient targets was determined via Alzdata database, and finally the core targets were analyzed. Molecular docking analysis and molecular dynamics (MD) simulation was further used in order to determine the binding affinity between the active ingredients and the core targets. Through a network pharmacological approach, we identified key genes in cytoscape. <em>C. pluricaulis</em> targets 30 signaling pathways and diseases associated with AD. Based on the overall results of the present study, it was found that all 4 bioactive compounds were identified to target GSK3β, and protein-ligand complex was shown to be stable for 100 ns in a molecular dynamics simulation and exhibited relativistic conformational dynamics on the trajectory. Thus, the present study suggests that modulating GSK3β with natural compounds is an attractive approach in treating AD and other neurodegenerative diseases after required validation.</div></div>","PeriodicalId":21919,"journal":{"name":"South African Journal of Botany","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142528571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The nitrogenous metabolites of pea (Pisum sativum L.): A phytochemical and pharmacological overview","authors":"John Refaat Fahim","doi":"10.1016/j.sajb.2024.10.027","DOIUrl":"10.1016/j.sajb.2024.10.027","url":null,"abstract":"<div><div><em>Pisum sativum</em> L. (pea) is a rich leguminous source of many primary and secondary metabolites that underpin its nutritional, health-promoting, and medicinal traits. This plant flourishes in a symbiotic relationship with rhizobia that inhabit its root nodules, giving rise to biological nitrogen fixation aptitude. In exchange for sugars produced by pea, such soil bacteria help convert atmospheric nitrogen into ammonium compounds that are then utilized by pea plants. This nitrogen-fixing capacity has therefore allowed different parts of <em>P. sativum</em> to accumulate structurally diverse nitrogenous principles, including proteinogenic and non-proteinogenic amino acids, oligopeptides, polypeptides, lectins, biogenic amines, alkaloids, nitrogenous bases, indoles, phospholipids, and cerebrosides. A considerable amount of research has also unveiled the protective and therapeutic potential of many of these metabolites against microbial infections, oxidative stress-related disorders, inflammation, and metabolic diseases (e.g. diabetes, hyperlipidemia, hypercholesterolemia, obesity, hypertension, and renal fibrosis), while some others revealed allelopathic, antifeedant, and insect repellent properties. Therefore, this review offers a comprehensive overview on the chemical diversity and pharmacological aspects of various nitrogen-containing metabolites produced by <em>P. sativum</em>; highlighting their contribution to the health outcomes of this valued herb and its possible application in future phytotherapy and drug development.</div></div>","PeriodicalId":21919,"journal":{"name":"South African Journal of Botany","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142527483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Domestication of ornamental plants: Breeding innovations and molecular breakthroughs to bring wild into limelight","authors":"Vipasha Verma ,&nbsp;Megha Katoch ,&nbsp;Payal Kapoor ,&nbsp;Aparna Misra ,&nbsp;Bhavya Bhargava","doi":"10.1016/j.sajb.2024.10.011","DOIUrl":"10.1016/j.sajb.2024.10.011","url":null,"abstract":"<div><div>Wild ornamental plants offer a rich reservoir of genetic resources, essential for developing new, improved cultivars. Domestication of wild ornamental plant species transforms them into distinct cultivars with enhanced attributes, imperative for enhancing the diversity, adaptability of cultivated plants and addressing the evolving demands of the floriculture industry. This review offers a novel perspective on the domestication of wild ornamentals by highlighting advancements in breeding methods, genomics, genetic engineering, and cutting-edge technologies like CRISPR-Cas9. The review underscores the importance of breeding techniques such as selection, hybridization, and QTL mapping in developing ornamental plants with desirable traits, providing a strong foundation for successful domestication. Also, it emphasizes the role of tissue culture techniques in accelerating the breeding process under controlled conditions, ensuring rapid propagation and consistency in plant quality. A significant focus is placed on the use of advanced genomic approaches, including <em>de novo</em> assembly and annotation, comparative genomics and transcriptomics, pangenomics, and epigenomics, in identifying and characterizing domestication related genes. The review also discusses the transformative potential of genetic engineering in developing superior ornamental cultivars with enhanced traits. Furthermore, the application of CRISPR-Cas9 is highlighted as a powerful tool for <em>de novo</em> domestication, enabling precise gene modifications that can accelerate the development of ornamental plants with improved aesthetics, stress resistance, and other desirable attributes. Taken together, integrating these innovative tools represent a significant leap forward, offering new possibilities for domestication of wild ornamentals.</div></div>","PeriodicalId":21919,"journal":{"name":"South African Journal of Botany","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142527484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genus Lepidium L. (Brassicaceae), A comprehensive review of botany, traditional uses, pharmacology, phytochemistry and the possible toxicities","authors":"Hossein Bakhshi Jouybari ,&nbsp;Tayebeh Amini ,&nbsp;Fatemeh Mirzaee","doi":"10.1016/j.sajb.2024.09.068","DOIUrl":"10.1016/j.sajb.2024.09.068","url":null,"abstract":"<div><div><em>Lepidium</em> L<em>.</em> an important weedy plant of Brassicaceae family, comprises approximately 265species which are endemic to all continents. Since ancient time, the therapeutic potential of <em>Lepidium</em> species were fully recognized and traditionally used in different parts of the world such as Asian, African and American countries. This review aimed to provide comprehensive information about the botanical characteristics, traditional uses, pharmacology, phytochemistry and toxicology of <em>Lepidium</em>, to discover therapeutic potentials of different <em>Lepidium</em> species for further research. The information available on <em>Lepidium</em> species were collected from scientific databases such as Science Direct, Google Scholar, Scopus, Web of Science, PubMed and some traditional texts. There are several promising bioactivities for this genus that some of them have long been recognized in traditional medicine such as coagulant, biofumigant, hepato-renal protective activities and fertility enhancement. To date, several types of phytochemicals isolated and identified from <em>Lepidium</em> species including glucosinolate, alkaloid, alkamide, macamide, mucilage, phytosterol, flavonoid and essential oil. A significant number of glucosinolates and their hydrolysis products (isothiocyanates), have been isolated from <em>Lepidium</em> species with potential pharmacological activities including anti-carcinogenic and chemoprotective activities with different mechanism of actions. Some studies on the safety assessment of L. <em>sativum</em> L., a common used <em>Lepidium</em> species, indicated some degree of toxicities in animal fetus which supposed to be related to its hydrolysis product constituents, named benzyl isothiocyanates (BITC). Moreover, exposure to goitrin, a glucosinolate degradation product from certain <em>Lepidium</em> species, may exert some adverse effects on thyroid glands, through the competitive inhibition of iodine uptake. It should be notice that despite the beneficial effects of Brassicaceae plants for reducing risk of different types of disease especially cancer, limit of the consumption of some <em>Lepidium</em> species as a routine meal should be considered.</div></div>","PeriodicalId":21919,"journal":{"name":"South African Journal of Botany","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142527486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Azospirillum brasilense and Azospirillum argentinense inoculation and salt stress modify antioxidant enzymes profile during in vitro rooting of jojoba","authors":"Ana J. Gonzalez ,&nbsp;Mauro E. Yarte ,&nbsp;Berta E. Llorente ,&nbsp;Ezequiel E. Larraburu","doi":"10.1016/j.sajb.2024.10.017","DOIUrl":"10.1016/j.sajb.2024.10.017","url":null,"abstract":"<div><div>Jojoba (<em>Simmondsia chinensis</em>) is an evergreen dioecious shrub widely grown in many countries to obtain a liquid wax with several uses. Salt stress is one of the main abiotic stresses that negatively affects plant growth and development and reduces crop yield. Salt stress causes oxidative stress that leads to the formation of ROS (reactive oxygen species), which causes severe damage to cell structures by oxidation of cell membranes. However, the antioxidant enzyme system is also triggered as a defense mechanism during stress conditions. The antioxidant enzymes, such as peroxidase (PO), phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO), and catalase (CAT), are regularly engaged in controlling the ROS quantities. This work analyzes the effect of bacterization with <em>Azospirillum brasilense</em> Cd and <em>Azospirillum argentinense</em> Az39 on the adventitious rooting of jojoba under salinity stress. PO, PAL, PPO, and CAT activities were determined on days 0, 3, 6, 9, 12, and 15 of culture. <em>A. brasilense</em> Cd and <em>A. argentinense</em> Az39 promoted jojoba shoots rhizogenesis in salt stress conditions and modified the activity of antioxidant enzymes during the <em>in vitro</em> rooting process. The changes in enzymatic profiles observed could explain the better rooting abilities of jojoba-inoculated plants under salt stress conditions and the higher abiotic stress tolerance.</div></div>","PeriodicalId":21919,"journal":{"name":"South African Journal of Botany","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142528172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of differential exposure of UV-C and melatonin stress on the therapeutic potential of bioactive compounds in Moringa oleifera","authors":"Muhammad Naeem Bajwa ,&nbsp;Gouhar Zaman ,&nbsp;Muhammad Asad Ullah ,&nbsp;Muhammad Uzair Javed ,&nbsp;Anisa Andleeb ,&nbsp;Christophe Hano ,&nbsp;Bilal Haider Abbasi","doi":"10.1016/j.sajb.2024.10.024","DOIUrl":"10.1016/j.sajb.2024.10.024","url":null,"abstract":"<div><h3>Introduction</h3><div><em>Moringa oleifera,</em> widely recognized as a miracle tree, boasts a rich repository of bioactive metabolites and nutraceuticals. This study explores a biotechnological approach utilizing UV-C and melatonin elicitation for enhancing pharmaceutically significant secondary metabolites in <em>M. oleifera</em> callus cultures.</div></div><div><h3>Methods</h3><div>callus cultures were exposed to different UV-C exposure durations and various melatonin concentrations to assess their combined impacts on biomass, secondary metabolite synthesis, and antioxidant ability. Biomass yields for fresh weight (F.W) were measured for 10 μM melatonin (176. 18 g/L) and UV-C 60 min (172.17 g/L). Total phenolic production (TPP: 25.83 mg/L D.W) and total flavonoids production (TFP: 157.07 mg/L D.W) peaked at 15 μM melatonin. High-performance liquid chromatography facilitated metabolite quantification.</div></div><div><h3>Results</h3><div>Significant increases in total phytochemical production were observed, reaching 12.98 mg g^-1 D.W with 15 μM melatonin—an approximately 2.5-fold rise. UV-C exposure for 60 min yielded a two-fold increase (10.847 mg g^-1 D.W) compared to the control (5.155 mg g^-1 D.W). Metabolic profiling identified four phytochemicals (p-coumaric acid, chlorogenic acid, rutin, and apigenin) through HPLC. Melatonin at 15 μM demonstrated superior DPPH free radical scavenging activity (93.32 %) compared to the control (82.05 %). Additionally, FRAP (382.52 TAEC μM) and ABTS (535.8 TAEC μM) exhibited maximum values under 15 μM melatonin concentration.</div></div><div><h3>Conclusions</h3><div>This study provides a comprehensive approach for enhanced phytochemical production, offering insights into the comparative impacts of UV-C and melatonin elicitation on secondary metabolite pathways in <em>M. oleifera</em> callus cultures.</div></div>","PeriodicalId":21919,"journal":{"name":"South African Journal of Botany","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142528166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro propagation, SEM analysis, and genetic fidelity assessment using SCoT markers in Decalepis salicifolia, an endangered medicinal plant","authors":"Adla Wasi,&nbsp;Anwar Shahzad,&nbsp;Sabaha Tahseen","doi":"10.1016/j.sajb.2024.10.036","DOIUrl":"10.1016/j.sajb.2024.10.036","url":null,"abstract":"<div><div><em>Decalepis salicifolia</em> (Bedd. ex Hook.f.) Venter is an important medicinal plant endemic to south Western Ghats of India. The immense pharmacological properties of the plant is due to presence of number of biologically active compound especially 2‑hydroxy-4-methoxybenzaldehyde (2H4MB) which is a vanillin isomer. 2H4MB is used in the preparation of various bakery products. Due to the multiple uses of the plant, its commercial demand has increased significantly which has led to its over-exploitation. Because of over-exploitation, the natural population of <em>D. salicifolia</em> is rapidly disappearing, and is listed as critically endangered plant by IUCN. Therefore to meet the demand of the plant for commercial purposes as well as ensure the natural population is conserved, the <em>in vitro</em> propagation approach provides an efficient alternative. In the present study, maximum <em>in vitro</em> shoot proliferation was obtained by culturing the shoot tip on Murashige and Skoog's (MS) medium supplemented with a combination of 5 μM benzyladenine, 1.0 μM indole butyric acid, and 20.0 μM adenine sulphate. Microshoots were rooted effectively on half strength MS medium augmented with 2.5 μM indole-3-butyric acid. Various physiological parameters along with scanning electron microscopic analysis of leaves confirmed the successful adaptation of the plantlets to the natural environmental condition. Presence of 2H4MB <em>in vitro</em> raised plant was confirmed with the help of FTIR. SCoT marker analysis of mother and <em>in vitro</em> regenerated plant produced a high percentage of monomorphic bands hence establishing a clonal fidelity between the both. Thus <em>in vitro</em> propagation protocol developed for <em>D. salicifolia</em> can help us to reduce the exploitation pressure on the natural population of the plant and contributes in the ecorestoration, conservation, and cultivation of the plant.</div></div>","PeriodicalId":21919,"journal":{"name":"South African Journal of Botany","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142528170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis and transcription factors response to transplanting and topping time of upper leaf in tobacco (Nicotiana tabacum L.)","authors":"Xianbo Zhao ,&nbsp;Feiyue Yuan ,&nbsp;Yiqiang Chen ,&nbsp;Ye Zhang ,&nbsp;Yiqi Zhang ,&nbsp;Yongan Zhang ,&nbsp;Shunhua Ji ,&nbsp;Zhuangyi Zhang ,&nbsp;Li Gu ,&nbsp;Lijuan Liao ,&nbsp;Tiedong Liu","doi":"10.1016/j.sajb.2024.10.013","DOIUrl":"10.1016/j.sajb.2024.10.013","url":null,"abstract":"<div><div>Tobacco, as an annual industrial crop, is significantly affected by agronomic practices such as transplanting and topping, which can influence the yield and quality of the upper leaves. However, the processes by which transcription factors integrate both agronomic practices remain unclear. In this study, eight treatments were designed based on varying transplanting and topping periods. RNA-seq was employed to analyze the transcriptional expression and identify key differentially expressed genes (DEGs) and transcription factors (TFs). Subsequently, a rigorous quality inspection and data cleaning process yielded 1,302,152,358 high-quality reads. The comparative analysis of 8 treatments identified a total of 4265 DEGs. Utilizing K-means analysis, these DEGs demonstrated 9 distinct expression patterns. GO and KEGG annotation revealed a significant association of these DEGs with photosynthesis and secondary metabolism. 10 hub genes including <em>AFC2, SIGB, PGSIP8, BIG5, OSP1, RL13, PDC1, NtORF</em> and <em>EBG</em> were identified through WGCNA analysis. 41 differentially expressed TFs were identified through transcription factor analysis. Among them, 10 TFs namely <em>ABR1, ERF26, HAT5, HSF24, LUH, AGL8, TIFY5A, TIFY10A, WRKY4</em>, and <em>WRKY40</em> are directly associated with plant stress resistance. 5 TFs such as <em>ERF110, IDD7, ATB40, NAC81</em>, and <em>NAC83</em> have been identified as positive regulators of growth and development, while 3 TFs such as <em>BH130, TCP4</em>, and <em>WRKY53</em> have been found to negatively impact growth and development. Furthermore, 4 TFs have been implicated in hormone and light signaling pathways, namely <em>CIGR1, BLH1, WRKY42</em>, and <em>EIN4</em>. The identified hub genes and TFs suggest that early transplanting and topping (B1D1C) contribute to enhanced stress resistance in tobacco. This may result in more developed stomata on tobacco leaves, as well as an increase in leaf thickness and a reduction in leaf size.</div></div>","PeriodicalId":21919,"journal":{"name":"South African Journal of Botany","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142528167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aqueous extracts of Physalis peruviana L. fruit promote apoptosis by overexpression of caspases 9/3 and balancing the redox profile of cutaneous melanoma cells","authors":"Alana Patrícia da Silva ,&nbsp;Gilnei Bruno da Silva ,&nbsp;Daiane Manica ,&nbsp;Filomena Marafon ,&nbsp;Rafael Antonio Narzetti ,&nbsp;Paula Dallagnol ,&nbsp;Letícia de Souza Matias ,&nbsp;Jullye Gavioli ,&nbsp;Greicy Cristine Kosvoski ,&nbsp;Sabine De Rocco Donassolo ,&nbsp;Francielle do Rocio da Luz ,&nbsp;Gilbrair Paulo de Carvalho ,&nbsp;Ariane Zamoner Pacheco de Souza ,&nbsp;Clevison Luiz Giacobbo ,&nbsp;João Paulo Bender ,&nbsp;Odinei Fogolari ,&nbsp;Luciano Tormen ,&nbsp;Margarete Dulce Bagatini","doi":"10.1016/j.sajb.2024.10.015","DOIUrl":"10.1016/j.sajb.2024.10.015","url":null,"abstract":"<div><div><em>Physalis peruviana</em> L. (PP), commonly known as golden berry, is a fruit renowned for its nutritional and medicinal properties. This study explores the antineoplastic potential of PP fruit extracts at varying stages of maturation, focusing on their impact on cutaneous melanoma cells. We analysed aqueous extracts from PP fruits at three distinct ripening phases, emphasizing their phenolic composition and antioxidant properties. Our <em>in vitro</em> assessments reveal that these extracts not only induce apoptosis in melanoma cells but also significantly upregulate caspase 9 and caspase 3 expression, leading to reduced cell viability. Moreover, the extracts inhibit melanoma cell migration and effectively modulate redox balance, suggesting an intricate interplay with cellular oxidative processes. These findings highlight the potential of PP fruit extracts as adjuvant agents in melanoma treatment or as a foundation for novel anticancer drug development, targeting specific pathways in cutaneous melanoma. Given these promising results, we advocate for subsequent <em>in vivo</em> studies and clinical trials to further elucidate the therapeutic efficacy and mechanisms of these PP extracts, thereby advancing future cancer therapeutic strategies.</div></div>","PeriodicalId":21919,"journal":{"name":"South African Journal of Botany","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142528168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}