South African Journal of Botany最新文献

{"title":"Corrigendum to “Formulation and evaluation of physicochemical properties of Hypnea pannosa incorporated bathing bar for unveiling its potential impact on bioactive properties” [South African Journal of Botany, 167 (2024) 1-11]","authors":"B. Deepika , P. Ganesan , B. Sivaraman , N. Neethiselvan , P. Padmavathy","doi":"10.1016/j.sajb.2024.11.003","DOIUrl":"10.1016/j.sajb.2024.11.003","url":null,"abstract":"","PeriodicalId":21919,"journal":{"name":"South African Journal of Botany","volume":"175 ","pages":"Page 770"},"PeriodicalIF":2.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to 'Co-application of fungal metabolites and nanoparticles control bacterial wilt disease by regulating rhizosphere soil microbial communities'","authors":"Guoxing Luo , Guoyu Luo","doi":"10.1016/j.sajb.2024.10.038","DOIUrl":"10.1016/j.sajb.2024.10.038","url":null,"abstract":"","PeriodicalId":21919,"journal":{"name":"South African Journal of Botany","volume":"175 ","pages":"Page 771"},"PeriodicalIF":2.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction notice:In vitro propagation and assessment of genetic fidelity in Dioscorea deltoidea, a potent diosgenin yielding endangered plant","authors":"Romaan Nazir , Suphla Gupta , Abhijit Dey , Vijay Kumar , Muzamil Yousuf , Shahnawaz Hussain , Padmanabh Dwivedi , Devendra Kumar Pandey","doi":"10.1016/j.sajb.2024.10.043","DOIUrl":"10.1016/j.sajb.2024.10.043","url":null,"abstract":"","PeriodicalId":21919,"journal":{"name":"South African Journal of Botany","volume":"175 ","pages":"Page 772"},"PeriodicalIF":2.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142743499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of antihyperglycemic potentials of seven Centaurea L. species through in vitro studies and characterization of possible bioactive phenolics by LC–MS/MS and molecular docking approach","authors":"Çiğdem Kahraman ,&nbsp;Zekiye Ceren Arıtuluk Aydın ,&nbsp;Mustafa Abdullah Yilmaz ,&nbsp;Oguz Cakir ,&nbsp;Nadire Özenver","doi":"10.1016/j.sajb.2024.11.004","DOIUrl":"10.1016/j.sajb.2024.11.004","url":null,"abstract":"<div><div>The taxon <em>Centaurea</em> L., a natural wealth with a high endemism rate in Türkiye, has been traditionally used in treating diabetes. This study aimed to determine the diabetes-associated biological activities and phytochemical content of different solvent extracts of <em>Centaurea benedicta</em> (L.) L, <em>C. cariensis</em> Boiss. subsp. <em>microlepis</em> (Boiss.) Wagenitz, <em>C. drabifolia</em> Sm. subsp<em>. cappadocica</em> (DC.) Wagenitz, <em>C. iberica</em> Trev. ex. Sprengel, <em>C. solstitialis</em> L. subsp. <em>solstitialis, C. urvillei DC.</em> subsp<em>. stepposa</em> Wagenitz, and <em>C. virgata</em> Lam. via <em>in vitro</em> and <em>in silico</em> methods. The antihyperglycemic potentials were estimated by determining the extracts’ <em>α</em>-amylase, <em>α</em>-glucosidase, and DPP-4 enzyme inhibitory abilities. The antioxidant capacities along with the total phenolic and flavonoid contents of the plant extracts were estimated by various methods. An LC-MS/MS analysis was used for the quantitative determination of the phytochemical components of the active extracts. <em>In silico</em> docking approach pointed out possible substances accounting for biological activity. Evaluating the <em>α</em>-glucosidase inhibitory activity of the plant extracts, the methanol and ethyl acetate extracts of <em>C. urvillei</em> were identified as the most active plant extracts with the lowest IC₅₀ values on <em>α</em>-glucosidase (17.9 and 17.6 µg/mL, respectively). None of the tested extracts inhibited <em>α</em>-amylase and DPP-4. The most abundant compounds in the active extracts were achieved by the LC-MS/MS method. Among those, cosmosiin and cynaroside were identified as promising antihyperglycemic agents based on molecular docking studies, suggesting their potential accounting for <em>α</em>-glucosidase inhibitory activity. Alcoholic and hydroalcoholic plant extracts generally exerted high antioxidant capacity. To conclude, specifically <em>C. urvillei</em> extracts along with cosmosiin and cynaroside were determined as potential and promising antihyperglycemic agents. The traditional usage of <em>Centaurea</em> species was verified, leading to the design of forthcoming projects associated with the antihyperglycemic potential of <em>Centaurea</em> species.</div></div>","PeriodicalId":21919,"journal":{"name":"South African Journal of Botany","volume":"176 ","pages":"Pages 248-260"},"PeriodicalIF":2.7,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142744379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of the Chenopodium quinoa gibberellin oxidase gene family and its role in seed germination","authors":"Yang Feng ,&nbsp;Shiyi Wang ,&nbsp;Fenggen Guo ,&nbsp;Zhengjie Liu ,&nbsp;Wenhong Long","doi":"10.1016/j.sajb.2024.11.027","DOIUrl":"10.1016/j.sajb.2024.11.027","url":null,"abstract":"<div><div>Pre-harvest sprouting (PHS) is an urgent problem in the quinoa breeding process. Gibberellin oxidases are key enzymes in the biosynthesis and degradation of gibberellins, playing a significant role in the regulation of active gibberellins (GAs). In this study, 18 <em>CqGAox</em> genes were identified in quinoa and characterized according to phylogenetic relationships, gene structure, conserved motifs, codon use pattern, and expression patterns. Based on their phylogenetic relationships, the <em>CqGAox</em> genes were classified into four groups: <em>C19CqGA2ox, C20CqGA2ox, CqGA3ox</em>, and <em>CqGA20ox</em>. Evolutionary analyses showed that homologous <em>GAox</em> genes from Arabidopsis, rice, and quinoa have been subject to purifying selection during evolution. According to the codon use patterns, mutational stress may cause codon bias in <em>CqGAox</em> genes. Prediction of promoter cis-regulatory elements suggested that the <em>CqGAox</em> genes contained multiple elements that responded to phytohormones and stress. We further evaluated the transcriptional responses of <em>CqGAox</em> genes during quinoa seed germination and under low-temperature stress treatments of seedlings. Moreover, qRT-PCR was used to confirm the changes in <em>CqGAox</em> gene expression during quinoa seed germination under GA₃ and paclobutrazol (PAC) treatments. Compared with the control, expression of <em>CqGAox</em> genes was significantly altered by treatment with GA₃ or PAC. These findings indicated that the <em>CqGAox</em> genes are essential for regulating GA biosynthesis and degradation. This study lays the foundation for further investigations of the roles of <em>CqGAox</em> genes in GA-regulated quinoa seed germination and provides potential targets for addressing the challenge of quinoa PHS.</div></div>","PeriodicalId":21919,"journal":{"name":"South African Journal of Botany","volume":"176 ","pages":"Pages 219-230"},"PeriodicalIF":2.7,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142705617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-catalytic activity of novel poly(ethylene glycol) methacrylate hybridized silver nanomaterials","authors":"Natasha Anwar ,&nbsp;Mohib Shah ,&nbsp;Saad Haroon Anwar ,&nbsp;Muhammad Ishaq Ali Shah ,&nbsp;Amirzada ,&nbsp;Hammad Ahmad Jan ,&nbsp;Abeer Hashem ,&nbsp;Graciela Dolores Avila-Quezada ,&nbsp;Elsayed Fathi Abd-Allah","doi":"10.1016/j.sajb.2024.11.017","DOIUrl":"10.1016/j.sajb.2024.11.017","url":null,"abstract":"<div><div>The widespread extensive use of common antibiotics and increase in drug-resistant bacterial strains have attained focus on the search for novel and effective antiproliferative agents. This study uses Periploca aphylla Dcne's as reducing and stabilizing agents to produce stable poly (ethylene glycol) methacrylate hybridized silver nanomaterials (PEGMA-AgNPs) with controlled physicochemical and biological properties in an environment friendly manner.</div><div>The study revealed that poly (ethylene glycol) methacrylate (PEGMA) coating had no effect on the crystal structure of Periploca aphylla Dcne's capped silver nanoparticles (Pe-AgNPs) which shows that only physical interactions took place during capping.</div><div>Characterization techniques like UV–visible spectrophotometer (UV), Fourier transform infrared spectroscopy(FTIR), scanning electron microscope (SEM), energy dispersive X-ray (EDX), transmission electron microscope (TEM), Zeta potential (ZP), and dynamic light scattering (DLS) were used. Relationships between the physicochemical properties of Pe-AgNPs, including synthesis, temperature, pH, and salt were studied. The produced PEGMA-AgNPs, which had a spherical shape and a negatively-charged Zeta potential of -20.2 mV, were stable in colloidal form for six months, as evidenced by a single symmetric surface plasmon resonance (SPR) band at 425 nm.</div><div>Pe-AgNPs and PEGMA-AgNPs used against PC-3 human prostate adenocarcinoma cell line and in addition, they also exhibited up-regulated anti-proliferative activity to this cell line. These results showed that gels and films which can be loaded with PEGMA-AgNPs may have potential as antiproliferative systems. Catalytic activity of the Pe-AgNPs and hybrid system were tested by choosing the catalytic reduction of 4-nitrophenol (4-NP) as a model reaction. Both synthesized Pe-AgNPs and PEGMA-AgNPs exhibits catalytic activity for the reduction of 4-NP to 4-aminophenol. Surprisingly however among various kinds of Pe-Ag nano-composites investigated only those stabilized by PEGMA had high catalytic activity. The study revealed that PEGMA-AgNPs exhibited strong antioxidant, cytotoxic, anti-diabetic and antileishmanial properties.</div></div>","PeriodicalId":21919,"journal":{"name":"South African Journal of Botany","volume":"176 ","pages":"Pages 197-206"},"PeriodicalIF":2.7,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142706576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LED light modifies plant architecture, physiological parameters and cannabinoid content in three varieties of Cannabis sativa L.","authors":"Julián Eduardo Carranza-Ramírez ,&nbsp;Ana María Borda ,&nbsp;Liz Patricia Moreno-Fonseca","doi":"10.1016/j.sajb.2024.11.023","DOIUrl":"10.1016/j.sajb.2024.11.023","url":null,"abstract":"<div><div>Changes in the light spectrum elicit responses in development and secondary metabolite biosynthesis and storage. The aim of this study was to determine the effect of two light sources on the physiological and yield parameters of three varieties of <em>C. sativa</em> cultivated under greenhouse conditions. The non-psychoactive varieties Calotoweed, Higthcol, and Souce Cauca were treated with two light sources [LED1(100% white) and LED2 (90% red, 8% blue, &lt;1% far red)] during the vegetative phase. Under LED2, reductions in plant height (4%-26.7%), leaf area (21%-55%), and shoot dry mass (1.9%-30.3%) were observed; however, there was a higher distribution of biomass towards the inflorescences (40.1%-51.6%). No effect of the light type on the relative chlorophyll content was observed. In all three varieties grown under LED2, reductions in stomatal conductance (4.7%-27.4%), quantum efficiency of PSII (1%-11.7%), and electron transport rate (9.2%-15.8%) were observed compared to plants under LED1. There were no significant differences in dry flower yield between light types used or varieties. Under LED1, higher CBD contents (11.9%-13.4%) and CBD per gram of inflorescence (12.9 CBD g/inflorescence – 13.8 CBD g/inflorescence) were observed compared to LED2. Under LED2, the Calotoweed (0.5%) and Soucecauca (0.6%) varieties showed higher THC concentrations compared to plants under LED1. These results demonstrate that LED2 modified canopy architecture, resulting in more compact plants with greater biomass accumulation in the inflorescence; however, these plants exhibited limitations in photosynthetic performance, which decreased CBD production and increased THC production. In conclusion, the type of light not only modified plant architecture but also affected photosynthetic performance and the production of CBD and THC.</div></div>","PeriodicalId":21919,"journal":{"name":"South African Journal of Botany","volume":"176 ","pages":"Pages 231-240"},"PeriodicalIF":2.7,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142705618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional traits of Ziziphus mucronata below mistletoe-infected trees in a semi-arid African savanna","authors":"Tsitsi S. Maponga ,&nbsp;Hilton G.T. Ndagurwa ,&nbsp;Ed T.F. Witkowski","doi":"10.1016/j.sajb.2024.11.032","DOIUrl":"10.1016/j.sajb.2024.11.032","url":null,"abstract":"<div><div>Mistletoes are increasingly associated with elevated leaf litter, soil nutrients, and soil moisture, resources which influence variations in plant functional traits. Despite this recognition, variations in the functional traits of understorey plants with overstorey (host) tree mistletoe infection are yet to be examined. Here, we measured the different functional traits (height, stem diameter, canopy area, leaf area, specific leaf area, whole-leaf thickness, leaf dry matter content, and chlorophyll content) of <em>Ziziphus mucronata</em>, a dominant woody plant beneath mistletoe-infected <em>Vachellia karroo</em> trees in semi-arid savanna. Two-sample <em>t</em>-tests were used to compare host size, mistletoe infection intensity, and trait variables between low and high mistletoe-infected trees. The relationships between <em>Ziziphus mucronata</em> functional traits <em>vs.</em> host tree diameter and the number of mistletoes per tree were explored using regression analysis and visualized using a regression biplot based on a redundancy analysis (RDA). Host tree height, canopy area, and canopy volume were strongly positively (<em>r</em> &gt; 0.7, <em>p</em> &lt; 0.05) related to mistletoe infection intensity. While most of the traits did not vary with mistletoe infection, the chlorophyll content and leaf area of understorey <em>Z. mucronata</em> increased with host tree size, being greater beneath high than low mistletoe-infected trees. These variations are linked to changes in limiting resources such as light, soil nutrients, and soil moisture due to accumulation of mistletoes and increase in size as the host tree ages. As a result, the understorey plants shifted from being resource conservative to being acquisitive as limiting resources increased. The general lack of trait plasticity in understorey <em>Z. mucronata</em> suggests that plastic allocation responses may not be a general consequence of mistletoe infection.</div></div>","PeriodicalId":21919,"journal":{"name":"South African Journal of Botany","volume":"176 ","pages":"Pages 241-247"},"PeriodicalIF":2.7,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142724173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Allium sativum and Allium cepa offer excellent potential for introgression and production of allicin and high total soluble solids into closely related wild Alliums","authors":"Kuldip Jayaswall ,&nbsp;Deepesh Kumar ,&nbsp;Deepanshu Jayaswal ,&nbsp;Himanshu Sharma ,&nbsp;Sanjay Kumar ,&nbsp;Chibuikem Unamba","doi":"10.1016/j.sajb.2024.11.011","DOIUrl":"10.1016/j.sajb.2024.11.011","url":null,"abstract":"<div><div>High total soluble solids (HTSS) and high sulfur-containing compounds like allicin are highly demanded by industry due to their variety of pharmaceuticals, and ecologically friendly alternatives to synthetic preservation agents. Compared to wild Alliums, cultivated Alliums are a rich source of HTSS and allicin. For introgression of HTSS trait-related genes in wild Alliums and identification of HTSS containing <em>Allium cepa</em> genotypes, we mined 1178 AcSSR markers and evaluated 200 AcSSR in ten Alliums for polymorphism and cross-transferability. Out of 200 AcSSRs, 72 were polymorphic and cross-transferable in Alliums. Moreover, 96 <em>Allium cepa</em> has been used to identify HTSS-containing genotypes and validate the aforementioned 10 AcSSR markers. Using DNA genotyping based Jaccard dissimilarity-based NJ tree, these 96 <em>Allium cepa</em> were grouped into high total soluble solids (HTSS) and low total soluble solids (LTSS) groups. In addition, 96 <em>Allium cepa</em> populations have been divided into six (<em>K</em> = 6) and three (<em>K</em> = 3) groups based on a Bayesian model. From 96 <em>Allium cepa</em> populations, we identified diverse 9 subsamples of <em>Alliums cepa</em>, by application of the bioinformatics tool “power core”. Surprisingly, out of 9 <em>Alliums cepa</em> subsamples, 6 genotypes have LTSS, and 3 genotypes have HTSS traits. Since we were particularly interested in identifying genes coding for sulphur metabolism also, so we selected 50 differentially expressed genes based on their high expression in <em>Allium sativum</em> compared to <em>Allium cepa</em>. Of the 50 selected genes, 27 were found to be allinase and cystine metabolism genes, 15 were related to glutathione metabolis and 8 were involved in sulphur metabolism. Among 27 genes associated with allinase and cystine metabolism, 25, 18, and 24, as well as genes related to sulphur metabolism, 8, 7, and 8, and glutathione metabolism, 15, 11, and 10 transcripts of <em>Allium sativum</em> showed upregulation in relation to <em>Allium cepa, Allium fragrance</em>, and <em>Allium fistulosum</em> respectively. Real-time PCR expression profiling with a selected 10 sulfur metabolism-related coding genes confirms the authenticity of the RNA-Seq results. Therefore, <em>Allium cepa</em> genotypes which have HTSS, and <em>Allium sativum</em> which has high sulphur-containing compound-like allicin traits might be introduced into wild Allium using these AcSSR markers and genetic engineering approach.</div></div>","PeriodicalId":21919,"journal":{"name":"South African Journal of Botany","volume":"176 ","pages":"Pages 207-218"},"PeriodicalIF":2.7,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142706577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Antioxidant & Antidiabetic Activities of Iridoid glycoside Isolated from Stachytarpheta cayennensis (Rich) Vahl Leaf Extract: In vitro combined with molecular docking approach","authors":"O.S. Ajayi ,&nbsp;O.S. Balogun ,&nbsp;E.G. Fakola ,&nbsp;I.J. Olawuni ,&nbsp;N. October ,&nbsp;A.H. Adewole","doi":"10.1016/j.sajb.2024.11.008","DOIUrl":"10.1016/j.sajb.2024.11.008","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;div&gt;&lt;em&gt;Stachytarpheta cayennensis&lt;/em&gt; is a medicinal plant widely used in the treatment of inflammations, ulcer, fever, pain, hepatic, renal disorders, syphilis, gonorrhoea, pain, diabetes, and hypertension.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Purpose&lt;/h3&gt;&lt;div&gt;This study aimed at investigating the antioxidant and antidiabetic potentials of the chemical constituents of &lt;em&gt;Stachytarpheta cayennensis&lt;/em&gt; leaf and its solvent extracts using both in vitro and &lt;em&gt;in silico&lt;/em&gt; methods&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;The crude methanolic extract of &lt;em&gt;S. cayennensis&lt;/em&gt; and its solvents fractions were tested for the presence of secondary metabolites using standard methods while the total phenolic content (TPC) and total flavonoid content (TFC) were determined by colorimetric assays using the Folin-Ciocalteu's and aluminum chloride reagents respectively. Thereafter, the aqueous methanolic fraction was purified on column chromatography to give compound &lt;strong&gt;1&lt;/strong&gt; which was characterised using spectroscopic techniques. The pure isolate and solvents fractions of the extract were then evaluated for antioxidant and antidiabetic properties. Furthermore, &lt;em&gt;in silico&lt;/em&gt; antidiabetic activity of the isolated compound was established using Autodock.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;The phytochemical screening of the crude methanolic extract and the solvent fractions revealed the presence of terpenoids, flavonoids, tannins, alkaloids, glycosides, sterols and phenols. The highest TPC and TFC were observed in ethyl acetate fraction (117.88 ± 9.83 GAE mg/g and 869.16 ± 7.26 QUE mg/g) while n-hexane fraction had the lowest (69.73 ± 1.47 GAE mg/g and 139.19 ± 2.69 QUE mg/g). The aqueous methanolic fraction, fractionated on column chromatography led to the isolation of compound &lt;strong&gt;1&lt;/strong&gt;, an iridoid glycoside known as ipolamiide. The ethyl acetate fraction demonstrated the highest DPPH free radical scavenging antioxidant activity (IC&lt;sub&gt;50&lt;/sub&gt; of 25.78 ± 0.05 µg/mL) while n-hexane fraction exhibited the least (4.67 ± 0.06 µg/mL) respectively, while the isolated ipolamiide had IC&lt;sub&gt;50&lt;/sub&gt; value of 49.4 ± 2.18 µg/mL. The &lt;em&gt;i&lt;/em&gt;n vitro α-amylase and α-glucosidase inhibitory activity of ipolamiide expressed in IC&lt;sub&gt;50&lt;/sub&gt; were 0.041 ± 0.021 mg/mL and 0.129 ± 0.030 mg/mL respectively compared to the standard drug acarbose (0.0163 ± 0.001 mg/mL and 0.0735 ± 0.0223 mg/mL) for α-amylase and α-glucosidase respectively. The docking analysis showed that ipolamiide exhibited docking scores that were comparable to those of established antidiabetic drugs (rosiglitazone, acarbose, gliclazide, and metformin) when interacting with the selected protein targets. Remarkably, ipolamiide displayed the most favourable docking score of -7.1 when bound to the insulin-like growth factor 1 kinase target. The docking results were visualized using Discovery Studio, which unveiled key molecular interactions contributing to the docking scor","PeriodicalId":21919,"journal":{"name":"South African Journal of Botany","volume":"176 ","pages":"Pages 184-196"},"PeriodicalIF":2.7,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142724174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}