Soil Biology & Biochemistry最新文献

{"title":"Plant organ rather than cover crop species determines residue incorporation into SOC pools","authors":"Tine Engedal ,&nbsp;Veronika Hansen ,&nbsp;Jim Rasmussen ,&nbsp;Jakob Magid ,&nbsp;Carsten W. Mueller ,&nbsp;Sune Tjalfe Thomsen ,&nbsp;Helle Sørensen ,&nbsp;Lars Stoumann Jensen","doi":"10.1016/j.soilbio.2024.109616","DOIUrl":"10.1016/j.soilbio.2024.109616","url":null,"abstract":"<div><div>The implementation of cover crops has emerged as a promising approach to improve soil organic carbon (SOC) stocks, with particular emphasis on the perceived higher carbon use efficiency displayed by high-quality residues such as from leguminous plants. In this study, we explored how different cover crop residues, specifically from a legume and a grass cover crop, affects SOC formation and its distribution across various soil carbon pools. Over a 7-month period, we incubated ¹⁴C-labeled winter rye and hairy vetch residues in microcosms containing soils of varying soil fertility levels from a long-term field trial. We tracked the fate of carbon into free and occluded particulate organic matter (fPOM, oPOM), mineral-associated organic matter (MAOM), and carbon deposited outside the detritusphere.</div><div>Despite notable differences in C:N ratio, chemical composition, and turnover rate, similar SOC formation efficiency between vetch and rye within each plant organ (shoots and roots) was observed. Interestingly, the plant organ appeared to exert a greater influence on the fate of cover crop carbon than whether the crop was leguminous or non-leguminous. This phenomenon seemed to be closely related to the lignin content.</div><div>At medium soil fertility, we found that the largest proportion of cover crop residue C remained as MAOM (20% for shoots, 15–18% for roots), followed by fPOM (5–6% for shoots, 10–12% for roots) and oPOM (2.7–3.0% for shoots, 1.5–1.6% for roots). Notably, fPOM and oPOM exhibited opposite responses to residue quality, indicating functional distinctions between these often-pooled POM pools.</div><div>Soil fertility exerted minimal influence on overall respiration rate patterns or SOC formation, although it did affect oPOM formation efficiency, likely due to differences in soil aggregation.</div><div>In conclusion, our findings challenge the assumption regarding the superiority of N rich leguminous cover crop residues for enhancing SOC accrual in C pools believed to have longer persistence.</div></div>","PeriodicalId":21888,"journal":{"name":"Soil Biology & Biochemistry","volume":"200 ","pages":"Article 109616"},"PeriodicalIF":9.8,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142377460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhizosphere priming and effects on mobilization and immobilization of multiple soil nutrients","authors":"Jiayu Lu ,&nbsp;Jiangping Cai ,&nbsp;Feike A. Dijkstra ,&nbsp;Liming Yin ,&nbsp;Peng Wang ,&nbsp;Weixin Cheng","doi":"10.1016/j.soilbio.2024.109615","DOIUrl":"10.1016/j.soilbio.2024.109615","url":null,"abstract":"<div><div>Living roots and their rhizodeposition play a vital role in mediating soil organic carbon (SOC) decomposition and nutrient mobilization. It is virtually unknown how the rhizosphere effects on soil nutrient mobilization are connected with the rhizosphere priming on SOC decomposition. Here we investigated the rhizosphere effects of six grassland species (four grasses and two legumes) on soil nutrient mobilization and SOC decomposition with and without nitrogen (N) fertilization in a 95-day pot experiment. Plant nutrient acquisition, soil extractable nutrients, and net nutrient mobilization or immobilization were determined to evaluate the rhizosphere effect on soil nutrient dynamics. Primed SOC decomposition was measured as the difference in soil-derived CO₂–C between planted and unplanted treatments. Without N fertilization, all species consistently increased net phosphorus (P), sodium (Na), iron (Fe), and copper (Cu) mobilization and most species increased net N, sulfur (S), calcium (Ca), and zinc (Zn) mobilization and net potassium (K), magnesium (Mg), and manganese (Mn) immobilization compared to the unplanted soil. These results suggest that grassland species could induce both positive and negative rhizosphere effects on soil nutrient mobilization with different magnitude. With N fertilization, plant-induced net N mobilization increased, while plant-induced net P and S mobilization decreased. Further, plant biomass, plant N, P, and S acquisition, and plant-induced net N, P, and S mobilization (<em>i.e.</em>, net nutrient mobilization in excess of the unplanted control), were positively correlated with primed SOC decomposition across six species, indicating that the mobilization of organically bound nutrients (N, P, and S) was connected with the rhizosphere priming on SOC decomposition. In contrast, plant-induced net nutrient mobilization of base cations and micronutrients was not related to primed SOC decomposition. Overall, our results demonstrate that a substantial portion of nutrient availability stems from rhizosphere processes and is plant species-specific, and that nutrient release of N, P and S are closely connected with rhizosphere priming on SOC decomposition.</div></div>","PeriodicalId":21888,"journal":{"name":"Soil Biology & Biochemistry","volume":"199 ","pages":"Article 109615"},"PeriodicalIF":9.8,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142374610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extrinsic rather than intrinsic factors determine microbial colonization of deadwood","authors":"Julia Moll ,&nbsp;Claus Bässler ,&nbsp;François Buscot ,&nbsp;Björn Hoppe ,&nbsp;Nico Jehmlich ,&nbsp;Harald Kellner ,&nbsp;Sarah Muszynski ,&nbsp;Matthias Noll","doi":"10.1016/j.soilbio.2024.109608","DOIUrl":"10.1016/j.soilbio.2024.109608","url":null,"abstract":"<div><div>Deadwood decomposition is primarily attributed to wood-colonizing fungi and bacteria, driven mainly by intrinsic (e.g. tree species identity) rather than by extrinsic factors. A recent cross-ecosystem study, using gamma-sterilized wood blocks of different coniferous and deciduous tree species placed at 150 forest and 150 grassland sites, revealed that intrinsic factors most strongly influenced rate of decomposition. These results raised the question of whether the wood-colonizing microbial biodiversity follows similar assembly patterns. For this purpose, we used metabarcoding to analyse the fungal and bacterial communities colonizing the wood blocks. We discovered that the wood-colonizing communities were more strongly determined by extrinsic factors such as the ecosystem type and microclimate (air humidity, soil pH, soil moisture, soil temperature) than by intrinsic factors (tree species identity, wood pH, wood mass loss). Although overall these results seem to be more pronounced for fungi, both communities comprised highly specialized wood colonizers in both ecosystems. For instance, the fungal genus <em>Mycena</em> and the bacterial genus <em>Granulicella</em> were detected more frequently in forests, whereas <em>Exophiala</em> and <em>Sphingomonas</em> were more abundant in grasslands. Wood mass loss exhibited a stronger correlation with reduced fungal diversity, while bacterial richness displayed no association with mass loss, both within and across forest and grassland sites. However, the composition of both colonizers’ communities was consistently linked to wood mass loss. Our study suggests that the environment selects distinct wood-colonizing communities that differ greatly in their decomposition efficiency; this result highlights the importance of cross-ecosystem analyses to assess ecological patterns.</div></div>","PeriodicalId":21888,"journal":{"name":"Soil Biology & Biochemistry","volume":"199 ","pages":"Article 109608"},"PeriodicalIF":9.8,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142369586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil nematode community profiling using reference-free mito-metagenomics","authors":"Xue Qing ,&nbsp;Michał Karlicki ,&nbsp;Fan Guo ,&nbsp;Anna Karnkowska ,&nbsp;Hongmei Li","doi":"10.1016/j.soilbio.2024.109613","DOIUrl":"10.1016/j.soilbio.2024.109613","url":null,"abstract":"<div><div>Nematodes are ubiquitous and diverse components of soil ecosystems worldwide. The 18S-based metabarcoding is known to have low species-level resolution and introduce bias in PCR. The mito-metagenomics (MMG) approach involves directly sequencing pooled samples, yields numerous mitochondrial reads that can be assembled into full or partial mitogenomes. This method circumvents the challenges associated with PCR-based metabarcoding and hold significant promise in biodiversity and phylogeny study. However, a reference database is typically required to extract mito-reads/contigs and provide taxonomic or phylogenetic context, thereby limiting its applicability. In this study, we introduced a novel reference-free pipeline for MMG assembly and diversity estimation. This pipeline has been integrated into a snakemake workflow, enabling the generation of output that is readily useable for phylogeny reconstruction in a single run. The performance tests have indicated that this new approach surpasses reference-based methods in soil nematode community profiling. We demonstrated that assembly quality improves with increasing sequencing depth, recommending an average of 1–2 Gb per species to achieve acceptable MMG assembly. Our pipeline presents an opportunity to create high-resolution phylogenies and assess diversity for poorly understood taxa, including neglected microscopic eukaryotes.</div></div>","PeriodicalId":21888,"journal":{"name":"Soil Biology & Biochemistry","volume":"199 ","pages":"Article 109613"},"PeriodicalIF":9.8,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142369585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Very fine roots differ among switchgrass (Panicum virgatum L.) cultivars and differentially affect soil pores and carbon processes","authors":"Jin Ho Lee ,&nbsp;Tayler C. Ulbrich ,&nbsp;Maik Geers-Lucas ,&nbsp;G. Philip Robertson ,&nbsp;Andrey K. Guber ,&nbsp;Alexandra N. Kravchenko","doi":"10.1016/j.soilbio.2024.109610","DOIUrl":"10.1016/j.soilbio.2024.109610","url":null,"abstract":"<div><div>Switchgrass (<em>Panicum virgatum</em> L.) is a promising feedstock for biofuel production, with diverse cultivars representing several ecotypes adapted to different environmental conditions within the contiguous USA. Multiple field studies have demonstrated that monoculture switchgrass cultivation leads to slow to negligible soil carbon (C) gains, an outcome unexpected for such a deep-rooted perennial. We hypothesize that different switchgrass cultivars have disparate impacts on soil C gains, and one of the reasons is variations in physical characteristics of their roots, where roots directly and indirectly influence formation of soil pores. We tested this hypothesis at Great Lakes Bioenergy Research Center's research site in Michigan using two lowland cultivars (Alamo and Kanlow) and four upland cultivars (Southlow, Cave-in-Rock, Blackwell, and Trailblazer). Three types of soil samples were collected: 20 cm diameter (Ø) intact cores used for root analyses; 5 cm Ø intact cores subjected to X-ray computed tomography scanning used for pore characterization; and disturbed soil samples used for microbial biomass C (MBC) and soil C measurements. Path analysis was used to explore interactive relationships among roots, soil pores, and their impact on MBC, and ultimately, on soil C contents across six cultivars. The abundance of very fine roots (&lt;200 μm Ø) was positively associated with fractions of pores in the same size range, but negatively with distances to pores and particulate organic matter. Higher abundance of such roots also led to greater MBC, while greater volumes of medium pores (50–200 μm Ø) and shorter distances to pores increased MBC. Results suggest that the greater proportion of very fine roots is a trait that can potentially stimulate soil C gains, with pore characteristics serving as links for the relationship between such roots and C gains. However, at present, ten years of cultivation generated no differences in soil C among the studied cultivars.</div></div>","PeriodicalId":21888,"journal":{"name":"Soil Biology & Biochemistry","volume":"199 ","pages":"Article 109610"},"PeriodicalIF":9.8,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142369584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of wheat to arbuscular mycorrhizal fungi inoculation and biochar application: Implications for soil carbon sequestration","authors":"A.R.G. Mason ,&nbsp;A.J. Lowe ,&nbsp;C. Brien ,&nbsp;N. Jewell ,&nbsp;T.R. Cavagnaro ,&nbsp;M.J. Salomon","doi":"10.1016/j.soilbio.2024.109611","DOIUrl":"10.1016/j.soilbio.2024.109611","url":null,"abstract":"<div><div>The sequestration of atmospheric CO₂ in soil is suggested as an effective climate change mitigation strategy. Biochar application shows promise in this regard, while the role of fungi in soil carbon cycling and sequestration is also under investigation. Using a novel high-throughput plant phenomics approach, we explore the impact of arbuscular mycorrhizal fungi (AMF) inoculation and biochar application on wheat growth and soil carbon, guided by one of the leading global carbon credit schemes. Wheat was successfully colonised by AMF, achieving an average root length colonisation of 35.9%. We uncover an indirect fungal-mediated pathway to soil carbon sequestration, with mycorrhizal plants generating more biomass across all soil treatments without yield penalties, suggesting colonised plants deliver more plant derived carbon to the soil, potentially leading to long-term soil carbon gains. Conversely, fungal-driven carbon loss occurred, significantly reducing soil carbon accumulation in unamended soil, but not in biochar-amended soil, suggesting that biochar moderates fungal activity and positively impacts the soil carbon balance. While both biochar and AMF enhance plant growth, their direct effects on soil carbon are complex. Although biochar did not significantly increase soil carbon stocks beyond its own contribution, its ability to regulate fungal activity could play an important role in influencing soil carbon sequestration.</div></div>","PeriodicalId":21888,"journal":{"name":"Soil Biology & Biochemistry","volume":"199 ","pages":"Article 109611"},"PeriodicalIF":9.8,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142369588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of climate warming and exogenous nitrogen input on soil N2O emissions from mangroves","authors":"Weimin Song ,&nbsp;Yan Zhao ,&nbsp;Jian Zhou ,&nbsp;Jianxiang Feng ,&nbsp;Zhonglei Wang ,&nbsp;Guangxuan Han ,&nbsp;Elise Pendall ,&nbsp;Guanghui Lin","doi":"10.1016/j.soilbio.2024.109607","DOIUrl":"10.1016/j.soilbio.2024.109607","url":null,"abstract":"<div><div>The paucity of studies on nitrous oxide (N₂O) dynamics with rising temperatures and nitrogen (N)-based eutrophication makes it challenging to evaluate the role of mangroves in mitigating climate change. Here, a 3-year mesocosm experiment was conducted to investigate the effects of climate warming (+3 °C) and excessive N input (25 mg N L⁻¹) on soil N₂O emissions from two mangroves (<em>Avicennia marina</em> and <em>Bruguiera gymnorrhiza</em>). We found that warming and N input alone significantly increased soil N₂O emissions from both mangroves, while the interactive effects of warming and N input on soil N₂O emissions were affected by mangrove species. Warming mitigated the positive effect of N input on soil N₂O emissions from <em>A</em>. <em>marina</em>; and amplified the effect of N input on soil N₂O emissions from <em>B. gymnorrhiza</em>, suggesting that the response of soil N₂O emissions to these global change factors is species-dependent. Stable isotopic signature analysis revealed that both warming and N input significantly increased the relative contribution of nitrification to N₂O emissions from <em>A</em>. <em>marina</em>; whereas N input, rather than warming, significantly changed the relative contribution of nitrification in <em>B. gymnorrhiza</em>. This could be attributed to the differential changes in soil environmental conditions, plant growth and the microbial structure of the two mangroves. Overall, this study highlights the role of mangrove species in modifying the effects of warming and N input on soil N₂O emissions, which should be considered when accurately projecting N₂O emissions from mangroves. Furthermore, considering the low N₂O emissions from background sediments and the common N limitation across mangroves, our findings suggest that climate warming and exogenous N input may lead to a surge of N₂O emissions from mangroves, especially those that are seriously affected by human activities.</div></div>","PeriodicalId":21888,"journal":{"name":"Soil Biology & Biochemistry","volume":"199 ","pages":"Article 109607"},"PeriodicalIF":9.8,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142369589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the active bacteria involving glucose-triggered priming effect in soils with gradient N inputs","authors":"Shengxian Chen ,&nbsp;Junjie Guo ,&nbsp;Ruijia Guo ,&nbsp;Baiqing Huang ,&nbsp;Jian Huang ,&nbsp;Min Wang ,&nbsp;Qirong Shen ,&nbsp;Ning Ling ,&nbsp;Shiwei Guo","doi":"10.1016/j.soilbio.2024.109612","DOIUrl":"10.1016/j.soilbio.2024.109612","url":null,"abstract":"<div><div>The soil priming effect, which refers to the alteration of soil organic matter (SOM) decomposition due to labile carbon (C) inputs, is widely acknowledged for its impact on C storage in terrestrial ecosystems. However, the impact of chronic nitrogen (N) fertilizer on soil priming effect, particularly in agroecological systems, remains unclear. Here, we utilized soils subjected to varying levels of N fertilization (0, 140, 280, 470, and 660 kg N ha⁻¹ y⁻¹), which were collected from a long-term experimental site. Enzyme activity related to C, N, and phosphorus (P) acquisition was measured using the fluorometric method. Additionally, DNA-based stable isotope probing with ¹³C-labeled glucose was conducted to explore the role of active bacterial communities (16S rRNA gene analysis) on the priming effect in soils with different N fertilization histories. Glucose addition enhanced the decomposition of native SOM and induced positive priming effects in all soils, which were amplified by the historical N application level. Activity of C-related enzymes essential for soil C decomposition increased following glucose addition, which was positively correlated with the soil priming effect. Active bacterial taxa, primarily <em>Firmicutes</em>, <em>Actinobacteria</em>, and <em>Proteobacteria</em>, were capable of assimilating exogenous glucose-C or native SOM-C. Notably, bacteria assimilating glucose exhibited higher abundance-weighted average ribosomal RNA gene operon copy number than those assimilating SOM, indicating the role of r-strategists in accelerating SOC turnover and increasing C loss. These findings highlight the role of active microbial community attributes on the soil priming effects. This study provides new insights into the intricate processes of C transformation in soils subjected to long-term N management in agroecosystems.</div></div>","PeriodicalId":21888,"journal":{"name":"Soil Biology & Biochemistry","volume":"199 ","pages":"Article 109612"},"PeriodicalIF":9.8,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142369587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental evidence that poor soil phosphorus (P) solubility typical of drylands due to calcium co-precipitation favors autonomous plant P acquisition over collaboration with mycorrhizal fungi","authors":"Kurt O. Reinhart ,&nbsp;Lance T. Vermeire ,&nbsp;Chad J. Penn ,&nbsp;Ylva Lekberg","doi":"10.1016/j.soilbio.2024.109605","DOIUrl":"10.1016/j.soilbio.2024.109605","url":null,"abstract":"<div><div>Calcareous dryland soils are rich in precipitated phosphate and represent &gt;30% of Earth's land, yet the relative importance of phosphorus-acquisition strategies (P_AS) among plant species in these systems is not well known. No experiment has investigated potential interactions between varying amounts of added calcium carbonate (CaCO₃) and arbuscular mycorrhizal fungi (AMF) on plant performance and P_AS which could test for potential mechanisms without the limitations of <em>in-situ</em> comparisons along natural gradients. To fill this knowledge gap, we conducted an experiment with CaCO₃ addition, AMF inoculation, and three invasive and five native grassland plants. We expected an increase in soil [CaCO₃] of an alkaline subsoil to 1) reduce soil-available phosphorus (P), 2) reduce plant biomass and P uptake, and 3) shift P_AS toward increased root mining as Ca-bound P increases. The largest CaCO₃ addition reduced available P by as much as 57%. On average, the largest addition of CaCO₃ reduced total biomass of plants by 19% and plant uptake of P by 15%. The P_AS seemed to have changed, and CaCO₃ additions tended to increase an indicator of root exudation (shoot [Mn]) to mobilize Ca-bound P, suggesting plasticity for some inducible root mining, especially <em>Artemisia frigida</em> and <em>Poa secunda</em>. However, CaCO₃ and plant species interacted to affect shoot [Mn]. The invasive grass <em>Bromus tectorum</em> was superior at acquiring P (&gt; P uptake, &gt; shoot [Mn]) and thus tolerating low soluble P conditions. Rarely did AMF and CaCO₃ interact to affect plant biomass. When they did, mycorrhizal responsiveness did not increase where P was less available, suggesting AMF become less beneficial upon P and Ca coprecipitation. In dryland soils with less soluble P, plants are thus likely to rely more on root mining P_AS than mycorrhizal scavenging, except for the invasive forb <em>Euphorbia esula</em> that always benefitted from AMF inoculation.</div></div>","PeriodicalId":21888,"journal":{"name":"Soil Biology & Biochemistry","volume":"199 ","pages":"Article 109605"},"PeriodicalIF":9.8,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142369590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial-temporal patterns of foliar and bulk soil 15N isotopic signatures across a heterogeneous landscape: Linkages to soil N status, nitrate leaching, and N2O fluxes","authors":"Elizabeth Gachibu Wangari ,&nbsp;Ricky Mwangada Mwanake ,&nbsp;Tobias Houska ,&nbsp;David Kraus ,&nbsp;Hanna-Marie Kikowatz ,&nbsp;Benjamin Wolf ,&nbsp;Gretchen M. Gettel ,&nbsp;Lutz Breuer ,&nbsp;Per Ambus ,&nbsp;Ralf Kiese ,&nbsp;Klaus Butterbach-Bahl","doi":"10.1016/j.soilbio.2024.109609","DOIUrl":"10.1016/j.soilbio.2024.109609","url":null,"abstract":"<div><div>The natural abundance of plant and bulk soil ¹⁵N isotopic signatures provides valuable insights into the magnitude of nitrogen cycling and loss processes within terrestrial ecosystems. However, ¹⁵N isotopic signatures are highly variable in space due to natural and anthropogenic factors affecting N cycling processes and losses. To date, most studies on foliar and bulk soil ¹⁵N isotopic signatures have focused on N-limited forest ecosystems at relatively large spatial scales, while similar studies in N-enriched ecosystems at finer spatial scales are lacking. To address this gap and evaluate links between soil ¹⁵N isotopic signatures and ecosystem N cycling and loss processes (plant N uptake, N leaching, and gaseous loss), this study quantified foliar and bulk soil ¹⁵N isotopic signatures, soil physicochemical parameters, gaseous (N₂O), and hydrological (NO₃) N losses at 80 sites distributed across a heterogeneous landscape (∼5.8 km²). To account for the spatial-temporal heterogeneity, the measurements were performed in four campaigns (March, June, September 2022, and March 2023) at sites that considered different land uses, soil types, and topography. Results indicated that foliar and bulk soil ¹⁵N isotopic signatures were significantly (P &lt; 0.05) more enriched in arable and grassland ecosystems than forests, suggesting a more open N cycle with significant N cycling and losses due to higher N inputs from fertilizers. Similar to soil inorganic N, N₂O fluxes, and NO₃ leaching rates, landscape-scale foliar and soil ¹⁵N isotopic signatures varied widely spatially, particularly at grassland and arable land (−3 to 9.0‰), with bivariate and multivariate analyses also showing significant relationships between landscape-scale soil ¹⁵N isotopic signatures and the aforementioned parameters (r²: 0.29 to 0.82). Based on these relationships, our findings suggested that foliar and bulk ¹⁵N isotopic signatures may capture fine-scale areas with persistently high and low environmental N losses (N₂O fluxes and NO₃ leaching) within a heterogeneous landscape.</div></div>","PeriodicalId":21888,"journal":{"name":"Soil Biology & Biochemistry","volume":"199 ","pages":"Article 109609"},"PeriodicalIF":9.8,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142369626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}