Soil Biology & Biochemistry最新文献

{"title":"Hydrolase-oxidase responses mediate distinct carbon dynamics following wetland conversion to paddy versus upland systems","authors":"Lifei Su ,&nbsp;Yi Liu ,&nbsp;Lishan Tan ,&nbsp;Xiangling Zhou ,&nbsp;Ting Wang ,&nbsp;Yinyin She ,&nbsp;Jiafang Huang ,&nbsp;Yuanbin Cai ,&nbsp;Shihua Li ,&nbsp;Pingping Guo ,&nbsp;Min Luo","doi":"10.1016/j.soilbio.2025.109973","DOIUrl":"10.1016/j.soilbio.2025.109973","url":null,"abstract":"<div><div>Extensive conversion of natural wetlands to agricultural systems threatens their carbon storage capacity. Predicting post-conversion soil organic carbon (SOC) dynamics remains challenging, largely due to poorly characterized SOC decomposition patterns across conversion pathways. Given that hydrolases target labile polymers and oxidases degrade recalcitrant polymers, their differential responses may explain divergent SOC dynamics following wetland conversion. Through a global meta-analysis (424 observations from 113 studies), we assessed enzymatic responses following conversion to paddies or uplands. We found three key patterns: (i) Conversion to paddies increased hydrolase activities by 83 % (95 % CIs: 50–123 %) while maintaining stable oxidase levels (<em>p</em> &gt; 0.05), whereas conversion to uplands elevated both hydrolase [47 % (27–71 %)] and oxidase [54 % (27–88 %)] activities; (ii) Pre-conversion wetland biogeochemical properties (e.g., coastal salinity, peatland acidity) modulated enzymatic responses following conversion to uplands but not to paddies; and (iii) Conversion to uplands exhibited a rapid early-stage (0–30 years) increase in hydrolase activities, which subsequently reached a steady state. In contrast, conversion to paddies showed no discernible change in hydrolases initially, with a significant accumulation occurring only in the later period (30–150 years). Oxidase activities demonstrated a progressive increase in uplands over time but remained stable in paddies across all time periods. Critically, oxidase response negatively correlated with SOC contents, while hydrolase responses were positively linked to CO₂ emission fluxes. These relationships suggested that conversion to paddies may preserve SOC stocks despite increasing CO₂ emissions, while conversion to uplands may result in substantial SOC loss with relatively low greenhouse gas emissions. Our findings confirmed differential enzymatic responses across conversion pathways, providing enzyme-based insights for explaining post-conversion SOC dynamics in converted agricultural systems.</div></div>","PeriodicalId":21888,"journal":{"name":"Soil Biology & Biochemistry","volume":"211 ","pages":"Article 109973"},"PeriodicalIF":10.3,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145059702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined legacy effects of drought and diverse plant residues: Fate and priming effects of glucose","authors":"Rachel Wooliver ,&nbsp;Stephanie N. Kivlin ,&nbsp;Sindhu Jagadamma","doi":"10.1016/j.soilbio.2025.109983","DOIUrl":"10.1016/j.soilbio.2025.109983","url":null,"abstract":"<div><div>Building soil organic carbon (SOC) is critical for agroecosystem functioning and resilience to climate change. SOC storage is driven by the accumulation of C in microbial biomass and residues (characterized by C stabilization efficiency, or CSE), formation of mineral-associated organic carbon (MAOC), and priming of existing SOC, all of which are disrupted by drought. Ecosystem recovery after drought may be improved by plant diversity/composition. We used a two-phase microcosm study with stable carbon (C) isotope (¹³C as glucose) tracing in an agricultural soil (silt loam). First, we amended soils with cover crop residues of varying diversity/composition and imposed a 30-day dry-down. Second, we allowed soils to recover from drought, then added and tracked ¹³C-glucose. All cover crop residues marginally increased glucose-CSE by 4.19 % at 24 h after glucose addition, decreased the accumulation of glucose-C in the MAOC fraction by 27.9 % at six months, and strongly influenced bacterial and fungal diversity and composition at both timepoints. Drought history did not influence microbial communities, but intensified glucose-driven priming of existing SOC, leading to positive priming in soils with no or monoculture residues and negative priming in soils with five-species mixture residues. Overall, our results indicate that cover crop residues (regardless of diversity/composition) have the potential to improve stabilization of newly added simple C as microbial biomass and residues, but prevent accumulation of that C as MAOC in the longer-term. At the same time, diverse plant residues can reduce simple C-induced priming of existing SOC after drought. Though limited to a single agricultural soil, these results shed light on how plant diversity/composition influences soil responses to global change.</div></div>","PeriodicalId":21888,"journal":{"name":"Soil Biology & Biochemistry","volume":"211 ","pages":"Article 109983"},"PeriodicalIF":10.3,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145068048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Links between soil microstructure dynamics and carbon cycling in response to land use and climate change","authors":"Mengqi Wu ,&nbsp;Maxime Phalempin ,&nbsp;Thomas Reitz ,&nbsp;Evgenia Blagodatskaya ,&nbsp;Steffen Schlüter","doi":"10.1016/j.soilbio.2025.109982","DOIUrl":"10.1016/j.soilbio.2025.109982","url":null,"abstract":"<div><div>Land-use systems differ in the balance between organic carbon inputs and microbial mineralization, affecting long-term soil carbon storage. Perennial grasslands maintain continuous root growth without tillage, promoting the accumulation of stable soil microstructure and biopores. In contrast, annual croplands experience fallow periods and periodic plowing, which disturb soil microstructure and accelerate the mineralization of physically protected carbon. However, the strength of soil microstructural regulation on carbon cycling and its responses to climate change remains unclear. Here, we studied five land-use types (two croplands and three grasslands) under ambient and future climate scenarios over five years, starting from the fifth year after establishment. The future climate scenario reflected regional projections of increased temperature and modified precipitation regimes. Using deep-learning-based X-ray CT image segmentation, we found that grasslands consistently contained higher volumes of biopores, particulate organic matter (POM), and decaying roots due to sustained root activity and turnover. Croplands exhibited a higher relative amount of fresh root in spring probably due to the rapid early-season growth of annual species, reduced microbial activity during fallow periods, and lack of year-round root inputs. A typical grassland microstructure fully developed in topsoil (5–10 cm) after 4–5 years. Land-use differences in deep soil (35–40 cm) remained small even after 10 years. Microbial biomass carbon and extractable organic carbon were consistently greater in grasslands, whereas total organic carbon diverged more slowly. The future climate scenario primarily influenced heterotrophic respiration and labile carbon pools through soil moisture, but did not significantly alter topsoil microstructure or carbon pools. POM volume, rather than pore structure, was the key driver of carbon mineralization, as the aeration of these microbial hotspots was not limiting. These findings highlight the potential of microstructure characteristics like root channel density and root degradation indicators to quantify long-term ecosystem development including carbon storage.</div></div>","PeriodicalId":21888,"journal":{"name":"Soil Biology & Biochemistry","volume":"211 ","pages":"Article 109982"},"PeriodicalIF":10.3,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145043312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amino acid stable isotope fingerprinting places arbuscular mycorrhizal fungi close to other fungal functional groups","authors":"Andrey G. Zuev ,&nbsp;Maryline Calonne-Salmon ,&nbsp;Stéphane Declerck ,&nbsp;Kirstin K. Cavanaugh ,&nbsp;Melanie M. Pollierer","doi":"10.1016/j.soilbio.2025.109980","DOIUrl":"10.1016/j.soilbio.2025.109980","url":null,"abstract":"<div><div>Arbuscular mycorrhizal (AM) fungi are an important part of the soil microbial community. While their effects on soil properties and nutrient cycles are widely studied, their direct contribution to soil food webs and energy fluxes therein remains poorly explored. Biomass of extraradical mycelium and spores of AM fungi in soil can be an important source of nutrients in AM dominated systems, such as grasslands and forests in both temperate and tropical regions. The compound specific stable isotope analysis of carbon in individual amino acids (CSIA-AA of carbon) is able to distinguish ectomycorrhizal from saprotrophic fungi. Here, we tested whether the method can also separate AM fungi from the other fungal groups by their amino acid isotopic profiles. We measured the δ¹³C values of individual essential amino acids (δ¹³С_eAA values) for three species of AM fungi (<em>Rhizophagus aggregatus, R. intraradices</em> and <em>R. irregularis</em>), grown <em>in vitro</em> in bi-compartmented Petri plates separating the AM fungal-colonized host root from the hyphae and spores, allowing to obtain plant-free AM fungal material. The δ¹³С_eAA based fingerprinting showed a strong difference between AM fungi and plants, and a high overlap of two studied species (<em>R. aggregatus</em> and <em>R. intraradices</em>) with both ectomycorrhizal (ECM) and saprotrophic (SAP) fungi. While still clustering close to fungi in the fingerprinting approach, the δ¹³С_eAA values of one species, <em>R. irregularis</em>, were distinct from the other fungal taxa, suggesting differences in metabolic pathways. Our results highlight that AM fungi are isotopically similar to ECM and SAP fungi and can be differentiated from plants, allowing to integrate AM fungi in the quantification of fungal energy channels in soil food webs. Additionally our data indicate the need for further investigations on the amino acid metabolic processes and stable isotope fractionation across different taxa of AM fungi.</div></div>","PeriodicalId":21888,"journal":{"name":"Soil Biology & Biochemistry","volume":"211 ","pages":"Article 109980"},"PeriodicalIF":10.3,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145043736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methane sink function of grassland soil microbiomes - negative effects of intensive management persist three years after land-use extensification","authors":"Nils Volles ,&nbsp;Hauke Winter ,&nbsp;Verena Groß ,&nbsp;Milos Bielcik ,&nbsp;Tim Urich ,&nbsp;Steffen Kolb ,&nbsp;Sven Marhan","doi":"10.1016/j.soilbio.2025.109981","DOIUrl":"10.1016/j.soilbio.2025.109981","url":null,"abstract":"<div><div>Grassland soils are important methane (CH₄) sinks through CH₄ oxidation by methanotrophs, but intensive management with high nitrogen inputs and grazing densities reduces this potential. While long-term recovery of the CH₄ sink after land-use change is generally established, little is known about the short-term effects of reducing land-use intensity index (LUI) through extensive management in grassland. We did not find an effect on potential CH₄ oxidation rates (PMORs) and the abundances atmospheric CH₄-consuming methanotrophs after three years of LUI reduction (no fertilization, no grazing, and one mowing per year) in 45 intensively managed grassland sites located in three different pedoclimatic regions of Germany. However, we observed a decline in the abundance of CH₄ producing methanogens. Moreover, we found greater PMORs and higher abundance of Upland Soil Cluster γ (USCγ) methanotrophs on additional, historically low LUI sites. Soil bulk density (BD) decreased already after three years of LUI reduction and was even lower in historically low LUI sites. Strong correlations between the abundance of canonical methanotrophs and methanogens highlight a CH₄ filter function that was independent from LUI reduction across regions. Our study consistently shows that three years of LUI reduction are not sufficient to restore the CH₄ sink function of temperate grasslands. However, the lower BD and the decreased abundance of methanogens indicate that LUI reduction may affect the niche-determining boundary conditions for CH₄-cycling microorganisms in the long term.</div></div>","PeriodicalId":21888,"journal":{"name":"Soil Biology & Biochemistry","volume":"211 ","pages":"Article 109981"},"PeriodicalIF":10.3,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145043421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Similar mineral-associated organic carbon formation but distinct efficiencies by powdered wollastonite addition between two soils","authors":"Yongxue Yan ,&nbsp;Liming Yin ,&nbsp;Shaobin Yan ,&nbsp;Yunting Fang ,&nbsp;Ang Wang ,&nbsp;Feifei Zhu ,&nbsp;Yanfeng Bai ,&nbsp;Zhenhua Zhang ,&nbsp;Weidong Zhang","doi":"10.1016/j.soilbio.2025.109979","DOIUrl":"10.1016/j.soilbio.2025.109979","url":null,"abstract":"<div><div>Recent studies showed that application of silicates powders (<em>e.g.</em>, wollastonite) to soils can enhance soil organic carbon (SOC) sequestration. However, whether and how the formation of mineral-associated organic carbon (MAOC) is affected by this practice is largely unknown. Here, we added ¹³C-labeled glucose at a rate of 5 % SOC to a forest and a farmland soil with and without CaSiO₃ powder (analytical grade) addition (5 % of oven-dried soil mass) in a 60-day incubation experiment. Microbial carbon use efficiency (CUE) and biomass turnover were measured using a¹⁸O labelling method. We found that ¹³C-mineral associated organic carbon (¹³C-MAOC) was significantly increased by ∼ 171 % in the forest soil, and by ∼ 252 % in the farmland soil by CaSiO₃ addition. CaSiO₃ addition also significantly increased ¹³C-particulate organic carbon (¹³C-POC) by 156 % in the farmland soil. As such, the forest soil had a higher percentage of ¹³C-MAOC formed relative to ¹³C-POC than the farmland soil. The increased ¹³C-MAOC in the forest soil is likely related to the increased microbial CUE (128.5 %) due to increased pH (3.5 units). Despite no significant increase in microbial CUE, CaSiO₃ addition decreased glucose-derived CO₂ in the farmland soil relative to the forest soil. These results suggest that more glucose may have been utilized in the anabolic pathway by microorganisms, leading to higher MAOC formation efficiency in the farmland soil than the forest soil. Overall, our results highlight that CaSiO₃ addition can promote MAOC formation, but the efficiency may depend on soil type.</div></div>","PeriodicalId":21888,"journal":{"name":"Soil Biology & Biochemistry","volume":"211 ","pages":"Article 109979"},"PeriodicalIF":10.3,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145043422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distinct mechanisms of soil organic carbon formation in natural and legume-based grasslands on the Loess Plateau, China","authors":"Zi-Qiang Yuan ,&nbsp;Chao Fang ,&nbsp;Tian Ma ,&nbsp;Jiu-Ying Pei ,&nbsp;Xin Song ,&nbsp;Guang-Qian Yao ,&nbsp;Jordi Sardans ,&nbsp;Josep Penuelas ,&nbsp;Xiang-Wen Fang ,&nbsp;Feng-Min Li","doi":"10.1016/j.soilbio.2025.109978","DOIUrl":"10.1016/j.soilbio.2025.109978","url":null,"abstract":"<div><div>The conversion of degraded land into grassland, through either abandonment or the introduction of legumes, is recognized as an effective strategy for increasing soil organic carbon (SOC). However, the impacts of this land use change on plant-derived lignin phenol and microbial residue carbon (MRC) remain insufficiently understood. In this study, we investigated the effects of grassland development following cropland abandonment and legume establishment on these SOC sources. We analysed 30 abandoned-cropland grasslands (CGs) and 30 alfalfa-established grasslands (AGs) with revegetation ages ranging from 1 to 30 years located in the semiarid region of the Loess Plateau, China. Our findings revealed that CGs exhibited a linear increase in lignin phenol content and its contribution to SOC (from 7.2 to 12.5 mg g⁻¹ SOC); however, there was no corresponding increase in MRC. In contrast, AGs demonstrated increases in both lignin phenol and MRC contents, along with their contributions to SOC (from 4.8 to 11.8 mg g⁻¹ SOC and from 32.8 % to 44.4 % SOC, respectively). Enzymatic stoichiometry analysis indicated that both grassland establishment alleviated microbial carbon limitation; nevertheless, microbial nitrogen limitation persisted, with its intensity remaining unrelated to the duration of grassland establishment. Soil exchangeable magnesium and root biomass primarily influenced the lignin phenol contribution to SOC, whereas soil dissolved organic carbon and microbial biomass carbon mainly affected the MRC contribution across both grassland types. These results underscore the distinct mechanisms of SOC formation and regulation in natural and legume-based grasslands. To enhance soil carbon sequestration in this and comparable dryland regions, priority should be given to the introduction of leguminous plants and the alleviation of microbial nitrogen limitation, thereby increasing plant carbon inputs and improving microbial conversion efficiency.</div></div>","PeriodicalId":21888,"journal":{"name":"Soil Biology & Biochemistry","volume":"211 ","pages":"Article 109978"},"PeriodicalIF":10.3,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145043423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decadal nutrient addition reveals phosphorus limitation and its adaptive mechanisms in tropical rainforests","authors":"Qingshui Yu ,&nbsp;Mark A. Anthony ,&nbsp;Arthur Gessler ,&nbsp;Xiangping Tan ,&nbsp;Jiangling Zhu ,&nbsp;Chengjun Ji ,&nbsp;Zhiyao Tang ,&nbsp;Jingyun Fang","doi":"10.1016/j.soilbio.2025.109976","DOIUrl":"10.1016/j.soilbio.2025.109976","url":null,"abstract":"<div><div>Tropical rainforests on low-phosphorus soils are highly biodiverse and productive, playing a crucial role in climate change mitigation. However, the degree of phosphorus limitation and potential adaptation mechanisms of tropical rainforests remain unclear.</div><div>Here, we conducted a decade-long field experiment with nitrogen (N) and phosphorus (P) additions in primary and secondary tropical rainforests. We investigated growth responses of 2012 individual trees and explored how litter, soil, and microbes contribute to maintaining P availability for plants.</div><div>We found that the P addition alone enhanced tree growth in both rainforests. Adding P (alone or with N) increased the average leaf P concentrations of eight species but reduced P resorption efficiency (PRE), soil phosphatase activity, and fungal diversity in the two forests. Phosphorus addition triggered divergent responses in fungal community composition across both forests: characterized by an enrichment of ectomycorrhizal fungi (EMF) and a depletion of arbuscular mycorrhizal fungi (AMF). Crucially, EMF functional guilds differentiated: short-distance exploration types increased significantly, while long-distance types declined.</div><div>These findings reveal that tropical rainforests adapt to P limitation through microbially mediated strategies: enhanced soil phosphatase activity for organic P mineralization and shifts toward EMF functional groups specialized in P acquisition. Reduced PRE indicates lower reliance on internal P recycling under elevated P availability. This study underscores the importance of P availability in shaping the productivity of tropical rainforests, providing critical insights into their adaptive responses to nutrient limitations.</div></div>","PeriodicalId":21888,"journal":{"name":"Soil Biology & Biochemistry","volume":"211 ","pages":"Article 109976"},"PeriodicalIF":10.3,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145025439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low retention of added nitrogen at realistic levels of simulated nitrogen deposition in the Northern Chihuahuan Desert","authors":"Jennifer Holguin,&nbsp;Anthony J. Darrouzet-Nardi,&nbsp;Jennie R. McLaren","doi":"10.1016/j.soilbio.2025.109977","DOIUrl":"10.1016/j.soilbio.2025.109977","url":null,"abstract":"<div><div>Aridlands are expected to be sensitive to even low levels of nitrogen (N) deposition, yet most experiments apply N in amounts that greatly exceed deposition estimates. We performed a low, but realistic N addition (i.e., based on N deposition estimates) at three adjacent Chihuahuan Desert semi-arid grassland sites. For four years, we applied 2 and 4 kg N ha⁻¹ yr⁻¹. We also included two additional treatments: a labile carbon (C) addition to test whether pre-existing N deposition had already altered our study ecosystem, and a supplemental water treatment prompted by drought during the first two years. In the field, we measured plant responses (cover, community composition, diversity, foliar C and N), soil ecosystem properties (pH, C, N, and P pools), microbial biomass, and extracellular enzyme activities. Additionally, we performed two short-term laboratory incubations: one measuring net N mineralization, net nitrification, and net ammonification, and another measuring nitric oxide (NO) emissions. Overall, our N inputs rarely drove significant changes in our field measures (including plant and soil N pools). Supplemental water did not induce any N addition effects, presumably due to a concurrent drought. However, despite above-average summer rainfall in the final year, N addition responses were still not apparent. In contrast, our incubations demonstrated that N enrichment can suppress net N mineralization and nitrification, while increasing NO emissions. Our field and incubation findings suggest that aridlands may be insensitive to low N deposition levels. This insensitivity may be associated with N losses, which could limit N retention in these ecosystems.</div></div>","PeriodicalId":21888,"journal":{"name":"Soil Biology & Biochemistry","volume":"211 ","pages":"Article 109977"},"PeriodicalIF":10.3,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145026023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil amoeba richness drives local-scale bacterial and fungal community assembly","authors":"Haijing Hu ,&nbsp;Fenggang Luan ,&nbsp;Xiaodong Liu ,&nbsp;Yina Jiang ,&nbsp;Dianming Hu ,&nbsp;Haozhi Long ,&nbsp;Zhijun Zhai ,&nbsp;Junqing Yan ,&nbsp;Chaoyu Cui ,&nbsp;Haiyan Song ,&nbsp;Jianping Zhou ,&nbsp;Gang He ,&nbsp;Shuanglin Chen ,&nbsp;Danushka Sandaruwan Tennakoon ,&nbsp;Yang Gao","doi":"10.1016/j.soilbio.2025.109972","DOIUrl":"10.1016/j.soilbio.2025.109972","url":null,"abstract":"<div><div>Protists are the primary consumers of bacteria and fungi within the soil microbial food web. However, the role of protist diversity in shaping bacterial and fungal community assembly remains unclear. To address this gap, we investigated how different levels of myxomycete (amoeboid protist) diversity affect the assembly of bacterial and fungal communities through field observations and microcosm experiments. Results indicate that myxomycete species richness significantly influences the assembly processes of fungal communities at the local scale, rather than those of bacterial communities. Increased myxomycete richness promotes deterministic fungal assembly by enhancing homogeneous selection. Additionally, myxomycete richness influences bacterial-fungal co-occurrence in inter-domain networks, indirectly driving bacterial community turnover. These findings highlight the direct top-down control exerted by amoeboid protists on fungi and the indirect trophic interactions that shape bacterial communities. They underscore the role of protist diversity as a key driver of soil microbial community assembly and provide novel insights into the complexity of microbial food webs.</div></div>","PeriodicalId":21888,"journal":{"name":"Soil Biology & Biochemistry","volume":"211 ","pages":"Article 109972"},"PeriodicalIF":10.3,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145009493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}