Separations最新文献_第3页

The Development and Validation of Simultaneous Multi-Component Quantitative Analysis via HPLC–PDA Detection of 12 Secondary Metabolites Isolated from Drynariae Rhizoma 通过 HPLC-PDA 检测从黄连中分离出的 12 种次生代谢物的多组分同步定量分析方法的开发与验证

IF 2.6 4区工程技术

Separations Pub Date : 2023-12-18 DOI: 10.3390/separations10120601

Jin Sung Ahn, WanKyunn Whang

{"title":"The Development and Validation of Simultaneous Multi-Component Quantitative Analysis via HPLC–PDA Detection of 12 Secondary Metabolites Isolated from Drynariae Rhizoma","authors":"Jin Sung Ahn, WanKyunn Whang","doi":"10.3390/separations10120601","DOIUrl":"https://doi.org/10.3390/separations10120601","url":null,"abstract":"Drynariae Rhizoma (DR) is a functional food and traditional medicine that has been widely used for bone and joint disorders for thousands of years. In this study, 14 compounds were isolated from DR, and their structures were identified using UPLC/QTOF–MS, UPLC–ESI/LTQ–Orbitrap–HRMS, and 2D NMR and compared with those obtained in previous studies. An HPLC–PDA multi-component simultaneous quantitative determination method was developed for 12 of the 14 DR-derived compounds, excluding compounds with a content <1.5 mg. The developed HPLC method was validated based on linearity (r2 ≥ 0.999), limit of detection (0.01–0.65 μg/mL), limit of quantification (0.04–1.97 μg/mL), intra-day precision and accuracy ranges (0.06–2.85% and 95.03–104.75%, respectively), and inter-day precision and accuracy ranges (0.24–2.83% and 95.75–105.75%, respectively). The developed analysis method improved the resolution of compounds 4 and 5. In addition, this is the first quantitative analysis of compounds 7, 8, and 11 and the first simultaneous quantitative analysis of 12 compounds, including compounds 4, 7, 8, 10, 11, and 14. This study developed a rapid, accurate, and economical HPLC method for performing the simultaneous quantitative analysis of 12 secondary metabolites isolated from DR.","PeriodicalId":21833,"journal":{"name":"Separations","volume":"14 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139173449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green and Fast Extraction of Chitin from Waste Shrimp Shells: Characterization and Application in the Removal of Congo Red Dye 从废弃虾壳中绿色快速提取甲壳素：表征及在去除刚果红染料中的应用

IF 2.6 4区工程技术

Separations Pub Date : 2023-12-13 DOI: 10.3390/separations10120599

Fatma Zohra Gharbi, Nabil Bougdah, Y. Belhocine, N. Sbei, S. Rahali, Maamar Damous, M. Seydou

{"title":"Green and Fast Extraction of Chitin from Waste Shrimp Shells: Characterization and Application in the Removal of Congo Red Dye","authors":"Fatma Zohra Gharbi, Nabil Bougdah, Y. Belhocine, N. Sbei, S. Rahali, Maamar Damous, M. Seydou","doi":"10.3390/separations10120599","DOIUrl":"https://doi.org/10.3390/separations10120599","url":null,"abstract":"Due to their detrimental and carcinogenic effects, synthetic organic dyes pose significant environmental and health risks. Consequently, addressing the bioremediation of industrial wastewater containing these organic dyes has become an urgent environmental concern. The adsorption using low-cost and green materials is one of the best alternative techniques for the removal of dyes. This study aims to investigate the use of chitin to eliminate Congo red (CR), an anionic dye, from wastewater. The chitin was produced from shrimp shell in a quick and environmentally friendly manner by utilizing a co-solvent (glycerol/citric acid (GLC)). The resulting adsorbent was characterized through various techniques, including X-ray diffraction (XRD), scanning electron microscopy (SEM), and FT-IR spectroscopy. The effectiveness of CR removal with chitin was studied with respect to contact time, adsorbent dose, initial pH, equilibrium isotherms, and kinetic and thermodynamic parameters. It was observed that variations in the dye concentration and pH significantly influenced the removal of CR with chitin. Under optimal operating conditions (pH = 7, contact time = 130 min, temperature = 50 °C), the adsorption capacity reached 29.69 ± 0.2 mg/g. The experimental data revealed that CR adsorption onto a chitin adsorbent is better represented by a Langmuir isotherm.","PeriodicalId":21833,"journal":{"name":"Separations","volume":"30 37","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139006691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extraction and Analysis of Chemical Compositions of Natural Products and Plants 天然产品和植物化学成分的提取与分析

IF 2.6 4区工程技术

Separations Pub Date : 2023-12-09 DOI: 10.3390/separations10120598

Mengjie Zhang, Jinhua Zhao, Xiaofeng Dai, Xiumei Li

引用次数: 0

Study on Enhancement of Denitrification Performance of Alcaligenes faecalis 关于提高粪阿卡菌反硝化性能的研究

IF 2.6 4区工程技术

Separations Pub Date : 2023-12-08 DOI: 10.3390/separations10120597

Zicheng Luo, Shugen Hu, Yameng Zhang, Guanlong Yu, Yunhe Yang, Qing Li, Peng Duan

{"title":"Study on Enhancement of Denitrification Performance of Alcaligenes faecalis","authors":"Zicheng Luo, Shugen Hu, Yameng Zhang, Guanlong Yu, Yunhe Yang, Qing Li, Peng Duan","doi":"10.3390/separations10120597","DOIUrl":"https://doi.org/10.3390/separations10120597","url":null,"abstract":"Nitrogen pollution in water bodies presents a serious threat to ecosystems due to its role in eutrophication. In this study, the aerobic denitrifying bacterium Alcaligenes faecalis was used as a model microorganism to investigate the optimal operating conditions for nitrogen removal from nitrogen-containing wastewater by Alcaligenes faecalis under different aeration modes, microbial dosages and C/N ratios. The results showed that the optimal aeration mode for efficient bacterial denitrification was 10 min of aeration with a 30 min interval, and the total nitrogen removal reached 87.82%. At different bacterial doses, NO3−–N was completely denitrified and NO2−–N accumulation levels were reduced, all of which resulted in significant denitrification, and the final total nitrogen removal efficiencies reached 86.39–98.50%. With an increase in the C/N ratio, the pollutant removal performance of denitrifying bacteria increased. When the C/N ratio was 17, the final rates of NO3−–N, TN and COD removal were 100%, 98.50% and 96.13%, respectively. At lower C/N ratios, the growth and metabolism of microorganisms were inhibited and fewer electron acceptors were available during the denitrification process, which seriously affected denitrification performance. In this study, the denitrification performance of aerobic denitrifying bacterium Alcaligenes faecalis was explored in experiments using changes in aeration mode, microbial dosage and C/N ratio, and the optimal operating conditions of Alcaligenes faecalis for treating nitrogenous wastewater were indicated. This provides technical support for Alcaligenes faecalis in improving the remediation effect of nitrogenous wastewater and provides a theoretical basis for further in-depth research on the performance of Alcaligenes faecalis in the future.","PeriodicalId":21833,"journal":{"name":"Separations","volume":"29 12","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138587811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research Progress on Extraction, Separation, and Purification Methods of Plant Essential Oils 植物精油提取、分离和纯化方法的研究进展

IF 2.6 4区工程技术

Separations Pub Date : 2023-12-07 DOI: 10.3390/separations10120596

Weiwei Zhou, Jun Li, Xuefeng Wang, Ling Liu, Yun Li, Rui Song, Mengxue Zhang, Xiumei Li

引用次数: 0

The Effect of Paraburkholderia on the Nutrients in Eutrophic Lakes 副伞菌对富营养化湖泊中营养物质的影响

IF 2.6 4区工程技术

Separations Pub Date : 2023-12-06 DOI: 10.3390/separations10120595

Chengqi Tu, Yongsheng Chang, Xiaoshuang Dong, Wei Huang

{"title":"The Effect of Paraburkholderia on the Nutrients in Eutrophic Lakes","authors":"Chengqi Tu, Yongsheng Chang, Xiaoshuang Dong, Wei Huang","doi":"10.3390/separations10120595","DOIUrl":"https://doi.org/10.3390/separations10120595","url":null,"abstract":"Cyanobacterial blooms in freshwater bodies are mainly attributed to the excess loading of nutrients. The microbes in sediments may affect nutrient migration and transformation during the growth of cyanobacteria. This study focused on the role of Paraburkholderia disturbance in affecting the sediment nutrient conditions and further contributing to cyanobacterial community succession in Meiliang Bay, Lake Taihu. The dissolving phosphorus and fixing nitrogen of Paraburkholderia with different concentration and characteristic capabilities, as well as the impact on nutrients (nitrogen (N), phosphorus (P), iron (Fe), etc.) in eutrophic lakes were determined. The results indicated that the various forms of phosphorus in the sediments showed total phosphorus (TP) > inorganic phosphorus (IP) > iron/aluminum-bound phosphate (NaOH-P) > algal-available phosphorus (AAP) > organic phosphorus (OP) > calcium-bound phosphate (HCl-P). Additionally, it was observed that with higher values of Paraburkholderia (OD600), the higher the corresponding risk of endogenous nutrient release from the sediments into the overlying water (but more is not always better), especially for the solubilization of HCl-P. The diffusion fluxes of TP, total nitrogen (TN) and Fe at the sediment–water interface (SWI) were all positive in the bacteria only experiment, with maximum values of 0.64, 15.0 and 5.02 mg/(m2d), respectively. Additionally, it was interesting that Paraburkholderia were able to produce organic acids, causing a decrease in pH. Furthermore, glucose levels can seriously affect water quality, especially the reduction in dissolved oxygen (DO) (down to 0.01 mg/L), leading to a series of side effects that have a huge impact on cyanobacterial community succession. These results provide a theoretical basis for the microbial ecological factors in eutrophic lakes.","PeriodicalId":21833,"journal":{"name":"Separations","volume":"45 20","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138597460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-Dimensional Liquid Chromatography of Pulse Triacylglycerols with Triple Parallel Mass Spectrometry 脉冲三酰甘油的多维液相色谱法和三重平行质谱法

IF 2.6 4区工程技术

Separations Pub Date : 2023-12-05 DOI: 10.3390/separations10120594

W. Byrdwell, H. K. Kotapati

{"title":"Multi-Dimensional Liquid Chromatography of Pulse Triacylglycerols with Triple Parallel Mass Spectrometry","authors":"W. Byrdwell, H. K. Kotapati","doi":"10.3390/separations10120594","DOIUrl":"https://doi.org/10.3390/separations10120594","url":null,"abstract":"We analyzed ten pulses (the dried seeds of legumes), i.e., baby lima beans, black beans, black-eyed peas, butter beans, cranberry beans, garbanzo beans, green split peas, lentils, navy beans, and pinto beans, using three-dimensional liquid chromatography (3D-LC) with parallel second dimensions, LC × (LC + LC). We combined non-aqueous reversed-phase (NARP) chromatography as the first dimension separation, 1D, with argentation UHPLC for separation based on degree and location of unsaturation in the first second dimension, 2D(1), and multi-cycle NARP-UHPLC in the second second dimension, 2D(2). Pulses contained 1.9% to 2.7% lipids, except garbanzo beans, which contained 6.2% lipids. High-resolution, accurate-mass (HRAM) orbitrap mass spectrometry (MS) was used to perform lipidomic analysis of the 2D(2) and percent relative quantification, showing that the most abundant average triacylglycerol (TAG) molecular species across all pulses were PLL at 10.67% and PLLn at 10.45%. Common beans (Phaseolus vulgaris) were clustered together using principal component analysis (PCA), showing the highest levels of linolenic acid, C18:3, in molecular species such as PLnLn, LLnLn, and OLLn, with palmitic (P), C16:0, linoleic (L), 18:2, linolenic (Ln), 18:3, and oleic (O), 18:1, FAs. Calibration curves derived from interweaved sets of regioisomer standards allowed the absolute quantification of 1,2- and 1,3-regioisomers for a subset of TAGs.","PeriodicalId":21833,"journal":{"name":"Separations","volume":"118 4","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138599685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Self-Driven Sustainable Oil Separation from Water Surfaces by Biomimetic Adsorbing and Transporting Materials 利用仿生吸附和传输材料实现水表面的自驱动可持续油分离

IF 2.6 4区工程技术

Separations Pub Date : 2023-12-04 DOI: 10.3390/separations10120592

Leonie Beek, Wilhelm Barthlott, M. Mail, K. Klopp, Thomas Gries

引用次数: 0

Recent Advances in the Effects of Biochar on Constructed Wetlands: Treatment Performance and Microorganisms 生物碳对人工湿地影响的最新进展：处理性能和微生物

IF 2.6 4区工程技术

Separations Pub Date : 2023-12-04 DOI: 10.3390/separations10120593

Guanlong Yu, Jiajun Huang, Huifang Chen, Jundan Chen, Shiyong Ge, Jiaxin Liu, Dian Zhen

引用次数: 0

Resource and Energy Utilization of Swine Wastewater Treatment: Recent Progress and Future Directions 猪废水处理的资源和能源利用：最新进展与未来方向

IF 2.6 4区工程技术

Separations Pub Date : 2023-12-03 DOI: 10.3390/separations10120591

Xiangwei Meng, Ming Jin, Qianzi Feng, Aiqi Sha, Shunwen Bai, Xinyue Zhao

{"title":"Resource and Energy Utilization of Swine Wastewater Treatment: Recent Progress and Future Directions","authors":"Xiangwei Meng, Ming Jin, Qianzi Feng, Aiqi Sha, Shunwen Bai, Xinyue Zhao","doi":"10.3390/separations10120591","DOIUrl":"https://doi.org/10.3390/separations10120591","url":null,"abstract":"Livestock and poultry farming, as a crucial component of agricultural production, poses a substantial threat to the ecological environment due to the discharge of wastewater. In recent years, researchers have proposed various resource treatment technologies for livestock and poultry breeding wastewater. However, a comprehensive discussion regarding the limitations and avenues for optimizing resource utilization technologies for livestock and poultry farming wastewater treatment is notably absent in existing literature. This paper takes swine wastewater as an illustrative case and undertakes a review of the advantages, disadvantages, and optimization directions of resource treatment technologies, including physical and chemical technology, microbial metabolism, microbial electrochemistry, constructed wetlands, and microalgae-based techniques. Based on mass balance, the recovery rates of various treatment technologies are estimated, and it was found that microbial electrochemistry and constructed wetland techniques may become the mainstream for resource utilization in the future. Furthermore, this paper emphasizes that in addition to resource efficiency, the optimization of resource utilization technologies for swine wastewater should also focus on the following aspects: (1) striking a balance between environmental impact and economic benefits; (2) reducing the cost of resource and energy utilization; and (3) safeguarding environmental and ecological security.","PeriodicalId":21833,"journal":{"name":"Separations","volume":"23 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138605762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0