Separations最新文献_第2页

Optimization and Component Identification of Ultrasound-Assisted Extraction of Polyphenols from Coriander (Coriandrum sativum L.) and Evaluation of Polyphenol Content Changes and Antioxidant Activity During Storage 香菜（Coriandrum sativum L.）中多酚的超声辅助提取工艺优化、成分鉴定及贮藏过程中多酚含量变化及抗氧化活性评价

4区工程技术

Separations Pub Date : 2025-08-16 DOI: 10.3390/separations12080217

Heng Yuan, Chunzhi Xie, Yue Ma, Yingchun Miao, Xuehong Chen, Hao Gong, Jun Wang

{"title":"Optimization and Component Identification of Ultrasound-Assisted Extraction of Polyphenols from Coriander (Coriandrum sativum L.) and Evaluation of Polyphenol Content Changes and Antioxidant Activity During Storage","authors":"Heng Yuan, Chunzhi Xie, Yue Ma, Yingchun Miao, Xuehong Chen, Hao Gong, Jun Wang","doi":"10.3390/separations12080217","DOIUrl":"https://doi.org/10.3390/separations12080217","url":null,"abstract":"Coriander (Coriandrum sativum L.) has significant value in the food industry due to its unique flavor and health benefits. However, its polyphenol content and antioxidant activity have not been systematically analyzed during storage. This study optimized the extraction process of coriander polyphenols using ultrasound-assisted extraction combined with response surface methodology. The polyphenol composition was systematically identified, and changes in polyphenol content and antioxidant activity during storage were investigated. The optimal process conditions for extracting coriander polyphenols were determined as 40% ethanol concentration, 1:121 g/mL material-to-liquid ratio, 81 °C extraction temperature, and 10 min extraction time. This optimized protocol yielded 16.231 mg GAE/g, a 119.28% increase over conventional methods using the same raw material. Fifty polyphenolic compounds were identified using high-resolution mass spectrometry. The main types of polyphenols identified were quercetin, kaempferol, and hydroxycinnamic acid derivatives. Notably, 41 of these compounds were reported in coriander for the first time. In vitro tests revealed that coriander polyphenols exhibit potent antioxidant properties, with IC50 values of 73.43 μg/mL for DPPH and 82.15 μg/mL for ABTS. Furthermore, the polyphenol content and antioxidant capacity of coriander increased significantly during storage, with total phenolic content rising by 40.5%, DPPH activity by 32.5%, and ABTS activity by 56.5%. Key individual polyphenols showed differential changes: rutin continuously accumulated, while chlorogenic acid and ferulic acid exhibited an initial increase followed by a decrease. This study provides strong technical support for the use of coriander polyphenols in functional foods and medicines.","PeriodicalId":21833,"journal":{"name":"Separations","volume":"12 8","pages":"217-217"},"PeriodicalIF":0.0,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2297-8739/12/8/217/pdf?version=1755329746","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147333854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Separation of Multiple Trace Impurities in Drugs Using an Improved Twin-Column Recycling Chromatography System 改进双柱循环色谱系统分离药物中多种微量杂质

4区工程技术

Separations Pub Date : 2025-08-11 DOI: 10.3390/separations12080210

Junwei Fu, Feng Wei

{"title":"The Separation of Multiple Trace Impurities in Drugs Using an Improved Twin-Column Recycling Chromatography System","authors":"Junwei Fu, Feng Wei","doi":"10.3390/separations12080210","DOIUrl":"https://doi.org/10.3390/separations12080210","url":null,"abstract":"The acquisition of high-purity impurities is pivotal for structural identification and an origin analysis, thereby laying a critical foundation for subsequent toxicological evaluation, quality standard development, and process optimization. This study investigated the feasibility of using a solvent gradient twin-column recycling chromatography technique for the separation and purification of multiple trace impurities in iohexol. In this approach, a modifier with a weaker elution strength than the mobile phase is introduced between two chromatographic columns to form a step gradient solvent system. This gradient slows down the leading edge of the elution band relative to the rear edge, inducing a band compression effect that counteracts band broadening and enhances the chromatographic resolution. By optimizing parameters such as the mobile phase composition, elution mode, and modifier flow rate, three trace impurities were successfully separated and purified from iohexol. Their respective purities were improved from initial concentrations of 0.36%, 0.35%, and 0.15% to 97.82%, 91.56%, and 81.56%, respectively. Leveraging the band compression effect on the target components, the impurities were simultaneously purified and concentrated. These results demonstrate that the proposed method is highly effective for the rapid isolation and preparation of trace pharmaceutical impurities.","PeriodicalId":21833,"journal":{"name":"Separations","volume":"12 8","pages":"210-210"},"PeriodicalIF":0.0,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2297-8739/12/8/210/pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147332509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the Adsorption Characteristics of Spirulina Dry Powder Biomass for Rare Earth Element Praseodymium(III): Adsorption Isotherms, Kinetics, and Thermodynamics Analysis 螺旋藻干粉生物质对稀土元素镨（III）的吸附特性研究：吸附等温线、动力学和热力学分析

4区工程技术

Separations Pub Date : 2025-07-25 DOI: 10.3390/separations12080195

Zhi‐Yi Hu, Caixia Zhang, Qing Shu

{"title":"Study on the Adsorption Characteristics of Spirulina Dry Powder Biomass for Rare Earth Element Praseodymium(III): Adsorption Isotherms, Kinetics, and Thermodynamics Analysis","authors":"Zhi‐Yi Hu, Caixia Zhang, Qing Shu","doi":"10.3390/separations12080195","DOIUrl":"https://doi.org/10.3390/separations12080195","url":null,"abstract":"Aimed at developing an economical and efficient biosorbent for the adsorption and separation of rare earth ions, this study employed Spirulina dry powder biomass as a biosorbent to investigate its removal performance for Pr3+ in aqueous solutions. Experimental results demonstrated that under optimized conditions (pH = 5, adsorbent dosage = 2.0 g/L, initial Pr3+ concentration = 100 mg/L, and adsorption time = 60 min), the removal efficiency of Pr3+ reached 79.0%. FT-IR and XPS characterization confirmed the participation of various functional groups on the Spirulina surface in the adsorption process. When 0.1 mol/L HNO3 was used as the desorption agent, the desorption rate of Pr3+ from Spirulina reached 91.7%, demonstrating excellent regeneration performance. At different temperatures (298–318 K), the adsorption data were fitted using Langmuir, Freundlich, Dubinin–Radushkevich, and Redlich–Peterson models. Among them, the Langmuir model (R2 ranged from 0.993 to 0.999) provided the best fit, and the adsorption capacity of Spirulina for Pr3+ was in the range of 51.10 to 55.31 mg/g. Kinetic studies revealed that the pseudo-second-order model (R2 = 0.999) best described the adsorption process, with a rate constant of 0.054 g/(mg·min) (R2 was 0.999) at an initial Pr3+ concentration of 300 mg/L, indicating chemisorption-controlled behavior. Thermodynamic parameter analysis showed that within the experimental temperature range, ΔG0 < 0 and ΔS0 > 0, confirming that the adsorption process was spontaneous and endothermic. This study provides a novel technical approach for the green recovery of rare earth elements and highlights the potential of Spirulina biomass in rare earth resource recycling.","PeriodicalId":21833,"journal":{"name":"Separations","volume":"12 8","pages":"195-195"},"PeriodicalIF":0.0,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2297-8739/12/8/195/pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147332742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Role of Freezing Temperature in Modulating Chitosan Gel Structure and Evaporation Performance for Seawater Desalination 冷冻温度对壳聚糖凝胶结构和海水淡化蒸发性能的调节作用

4区工程技术

Separations Pub Date : 2025-07-24 DOI: 10.3390/separations12080193

Jiaonan Cai, Yong Bai, Fang Li

引用次数: 0

Simultaneous Determination of 23 Trans Fatty Acids in Common Edible Oils by Gas Chromatography-Mass Spectrometry 气相色谱-质谱联用测定常见食用油中23种反式脂肪酸

4区工程技术

Separations Pub Date : 2025-06-28 DOI: 10.3390/separations12070171

Yanping Cao, Xia Li, Kun Wang, Xianpeng Wu, Jie Zhang, Fengen Wang

{"title":"Simultaneous Determination of 23 Trans Fatty Acids in Common Edible Oils by Gas Chromatography-Mass Spectrometry","authors":"Yanping Cao, Xia Li, Kun Wang, Xianpeng Wu, Jie Zhang, Fengen Wang","doi":"10.3390/separations12070171","DOIUrl":"https://doi.org/10.3390/separations12070171","url":null,"abstract":"Trans fatty acids (TFAs) pose significant health risks, including cardiovascular disease and metabolic disorders. However, the lack of high-resolution, high-sensitivity, and high-throughput quantitative methods for TFA analysis has led to fragmented data on TFA content in edible oils, which constrains research on the quality assessment of edible oils. In this study, we developed a high-resolution and high-sensitivity gas chromatography-mass spectrometry method to simultaneously determine 23 TFA isomers. The method validation demonstrated good sensitivity, linearity, accuracy, and precision. Based on the proposed method, we analyzed 170 samples of 11 common edible oils, establishing a comprehensive TFA profile for each type. Ruminant fats (beef tallow, mutton tallow, butter) had high TFA levels (0.8–4.8 g/100 g), dominated by vaccenic acid (C18:1 t11) and conjugated linoleic acid, while vegetable oils (soybean, corn, peanut and sesame oil) exhibited lower concentrations (0.5–2.2 g/100 g), especially monounsaturated TFAs. Particularly, soybean oil was rich in C18:3 isomers, while shortening presented the closest similarity to sesame oil. Cluster analysis distinguished oils by TFA composition, highlighting low-TFA clusters (sunflower oil, pork lard, cream). In conclusion, the high-resolution, high-sensitivity, and high-throughput TFA quantification method developed in this study provides technical support for establishing characteristic TFA profiles in edible oils, while offering data support to further quality assessment.","PeriodicalId":21833,"journal":{"name":"Separations","volume":"12 7","pages":"171-171"},"PeriodicalIF":0.0,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2297-8739/12/7/171/pdf?version=1751094042","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147331888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sponge-like Modified White-Rot Fungi Adsorbent for Rapid Removal of Pb(II) and Cd(II) from Solution: Selective Performance and Mechanistic Insights 海绵状改性白腐菌吸附剂对溶液中Pb（II）和Cd（II）的快速去除：选择性能和机理研究

4区工程技术

Separations Pub Date : 2025-06-28 DOI: 10.3390/separations12070172

Chunxiao Wang, Zhi‐Rong Chen, Nana Wang, Jianqiao Wang, Rui He, Yu Chen, H. Nuhu, Hang Chen, Zhenguo Lin, Fan Mei-hua, Mingdong Chang

{"title":"Sponge-like Modified White-Rot Fungi Adsorbent for Rapid Removal of Pb(II) and Cd(II) from Solution: Selective Performance and Mechanistic Insights","authors":"Chunxiao Wang, Zhi‐Rong Chen, Nana Wang, Jianqiao Wang, Rui He, Yu Chen, H. Nuhu, Hang Chen, Zhenguo Lin, Fan Mei-hua, Mingdong Chang","doi":"10.3390/separations12070172","DOIUrl":"https://doi.org/10.3390/separations12070172","url":null,"abstract":"Heavy metal pollution, especially from Pb(II) and Cd(II), poses significant risks due to its persistence and bioaccumulation potential. Traditional removal methods face challenges like high costs and secondary pollution. This study developed a novel three-dimensional porous adsorbent XBS, derived from xanthate-modified Phanerochaete sordida YK-624 (a white-rot fungus), for the rapid and efficient removal of Pb(II) and Cd(II) from wastewater. Characterization showed that XBS has a sponge-like structure with abundant functional groups, significantly enhancing its adsorption capacity and kinetics. XBS achieved 96% Pb(II) and 32% Cd(II) removal within 1 min at a 0.25 g/L dose, reaching over 95% of the maximum adsorption capacity within 30 min for Pb(II) and 240 min for Cd(II). The maximum capacities were 224.72 mg/g for Pb(II) and 82.99 mg/g for Cd(II). Kinetic and thermodynamic analyses indicated a chemisorption-driven process, which was both endothermic and spontaneous. XBS exhibited high selectivity for Pb(II) over Cd(II) and other metals (Tl(I), Cu(II)), attributed to stronger covalent interactions with sulfur- and nitrogen-containing groups. Mechanistic analyses (XRD, FTIR, and XPS) revealed that removal occurs via ion exchange, complexation, and precipitation, forming stable compounds like PbS/CdS and PbCO3/CdCO3. Given its cost-effectiveness, scalability, and high efficiency, XBS represents a promising adsorbent for heavy metal remediation, particularly in Pb(II)-contaminated wastewater treatment applications.","PeriodicalId":21833,"journal":{"name":"Separations","volume":"12 7","pages":"172-172"},"PeriodicalIF":0.0,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2297-8739/12/7/172/pdf?version=1751099315","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147333862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Purification of Mogroside V from Crude Extract of Siraitia grosvenorii Using Boronic Acid-Functionalized Silica Gel and Its Hypoglycemic Activity Determination 硼酸功能化硅胶纯化罗汉果粗提物中苦参苷V及其降糖活性测定

4区工程技术

Separations Pub Date : 2025-05-22 DOI: 10.3390/separations12060135

Yanmei Xu, Laiming Li, Pingyi Zheng, Ran Zhao, Mengqi Cheng, Yanfang Su, Jinpeng Bao, Youxin Li

{"title":"Purification of Mogroside V from Crude Extract of Siraitia grosvenorii Using Boronic Acid-Functionalized Silica Gel and Its Hypoglycemic Activity Determination","authors":"Yanmei Xu, Laiming Li, Pingyi Zheng, Ran Zhao, Mengqi Cheng, Yanfang Su, Jinpeng Bao, Youxin Li","doi":"10.3390/separations12060135","DOIUrl":"https://doi.org/10.3390/separations12060135","url":null,"abstract":"Mogroside V crude extract from Siraitia grosvenorii has many pharmacological effects, such as anti-diabetes, antioxidant, etc. It is being used as a kind of natural sweetener in more and more countries. The improvement of Mogroside V purity can greatly promote the utilization value of Siraitia grosvenorii and the quality of related products. For this paper, a boronic acid-functionalized silica gel adsorbent (SiO2-GP-APBA) was synthesized and applied for the first time in the purification of mogroside V from the crude extract of Siraitia grosvenorii. It was demonstrated that it was 30–100 μm with 163.1 μmol/g of boronic acid groups on the surface of silica gel and stable at below 380.20 °C. Its maximum adsorption capacity to mogroside V was up to 206.74 mg/g at room temperature. After the saturated absorption from the crude extract of Siraitia grosvenorii in a pH 3 solution, 96.36% mogroside V could be released from SiO2-GP-APBA using a pH 7 aqueous solution, which was better than ethanol. The purity of mogroside V was significantly increased from 35.67% to 76.34%. Semi-preparative HPLC could further improve the purity of mogroside V to 99.60%. Additionally, the direct inhibition effect of the mogroside V on α-glucosidase was determined for the first time. Its inhibitory constant was 46.11 μM, indicating mogroside V was beneficial for the treatment of diabetes.","PeriodicalId":21833,"journal":{"name":"Separations","volume":"12 6","pages":"135-135"},"PeriodicalIF":0.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147331948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Enhanced Selective Separation of Pu(IV) and U(VI) Using Novel Diethylene Glycolamide Ligand 新型二乙二醇酰胺配体增强对Pu（IV）和U（VI）的选择性分离

4区工程技术

Separations Pub Date : 2025-04-23 DOI: 10.3390/separations12050106

Xiaoyun Guo, Junli Wang, Yao Liu, Haojun Zhao, Hui Wang, Wentao Wang, Baole Li, Taihong Yan

引用次数: 0

Synthesis of Geopolymer-Based Fenton-like Catalytic Tubular Membrane for Dye Wastewater Treatment 染料废水处理用地聚合物类fenton催化管状膜的合成

4区工程技术

Separations Pub Date : 2025-04-17 DOI: 10.3390/separations12040099

Pei Xiao, Qing Yang, Xingfa Deng, Kunyu Chu, Xuemin Cui

{"title":"Synthesis of Geopolymer-Based Fenton-like Catalytic Tubular Membrane for Dye Wastewater Treatment","authors":"Pei Xiao, Qing Yang, Xingfa Deng, Kunyu Chu, Xuemin Cui","doi":"10.3390/separations12040099","DOIUrl":"https://doi.org/10.3390/separations12040099","url":null,"abstract":"Membrane technology is widely used in various aspects of wastewater treatment; however, single membrane technology has a series of disadvantages, such as high selectivity, poor recycling performance, and susceptibility to contamination. In this study, a treatment method combining an advanced oxidation process and membrane separation technology was proposed, and a geopolymer-based Fenton-like catalytic tubular membrane (GFM) was prepared by using H2O2 as a blowing agent by the direct foaming method. It was shown that the optimum conditions for the preparation of the membrane were a water glass modulus of 1.8 M, the addition of foaming agent of 1 mL, and a thickness of the membrane of 6.5 mm, with a flux of 6942 L·m−2·h−1. Due to the characteristics of the tubular membrane, the possibility of adding hydrogen peroxide directly inside the membrane allows an optimal Fenton-like removal, which is better than outside the membrane, thus reducing the consumption of hydrogen peroxide. The tubular membrane has a multi-stage porous structure, high flux, and a high specific surface area (68.74 m2/g). The GFM/H2O2 Fenton-like system formed is capable of almost completely degrading all kinds of synthetic dyes under various stringent conditions, and the XRD, FTIR, and TG analyses and cycling tests showed that the GFM has excellent stability and a significant advantage in terms of reusability.","PeriodicalId":21833,"journal":{"name":"Separations","volume":"12 4","pages":"99-99"},"PeriodicalIF":0.0,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2297-8739/12/4/99/pdf?version=1744891779","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147331597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening and Isolating Acetylcholinesterase Inhibitors from Olea europaea L. Fruit Using Ultrafiltration–Liquid Chromatography Coupled with High-Speed Counter-Current Chromatography 超滤-液相色谱-高速逆流色谱法筛选分离油橄榄果实中乙酰胆碱酯酶抑制剂

4区工程技术

Separations Pub Date : 2025-04-12 DOI: 10.3390/separations12040096

Xingcui Wang, Yingshan Zhang, Jules Muhire, Duolong Di, Xinyi Huang, Dong Pei

{"title":"Screening and Isolating Acetylcholinesterase Inhibitors from Olea europaea L. Fruit Using Ultrafiltration–Liquid Chromatography Coupled with High-Speed Counter-Current Chromatography","authors":"Xingcui Wang, Yingshan Zhang, Jules Muhire, Duolong Di, Xinyi Huang, Dong Pei","doi":"10.3390/separations12040096","DOIUrl":"https://doi.org/10.3390/separations12040096","url":null,"abstract":"Alzheimer’s disease (AD) is a progressive neurodegenerative condition and one of the most prevalent types of dementia in older adults. Currently, the primary drugs used to treat AD are acetylcholinesterase (AChE) inhibitors. The development of natural substances has become a research hotspot due to the high number of adverse effects of synthetic drugs. In this study, a new assay based on ultrafiltration–liquid chromatography–high-speed counter-current chromatography (UF-HPLC-HSCCC) was developed for the rapid screening and identification of AChE inhibitors from Olea europaea L. fruit. In this research, we screened and isolated two AChE inhibitors from O. europaea fruit extracts, identified by EI-MS and NMR as secologanoside and oleuroside-11-methyl ester. These compounds were identified for the first time from O. europaea and found to possess AChE inhibitory activity using an in vitro AChE inhibition assay and molecular docking. The IC50 values of the two compounds were 0.76 ± 0.04 mM and 1.08 ± 0.05 mM. The results demonstrated that secologanoside showed better AChE inhibition activity than oleuroside-11-methyl ester, suggesting that this compound is a promising AChE inhibitor. At the same time, the results showed that the combination of UF-HPLC- HSCCC provides a powerful tool for screening and isolating AChE inhibitors in complex samples.","PeriodicalId":21833,"journal":{"name":"Separations","volume":"12 4","pages":"96-96"},"PeriodicalIF":0.0,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147334012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1