Small Ruminant Research最新文献

{"title":"The impact of small ruminant lentiviruses infections on milk yield and milk quality traits in intensively reared dairy sheep","authors":"Aphrodite I. Kalogianni,&nbsp;Athanasios I. Gelasakis","doi":"10.1016/j.smallrumres.2024.107424","DOIUrl":"10.1016/j.smallrumres.2024.107424","url":null,"abstract":"<div><div>Despite small ruminant lentiviruses (SRLV) are widespread causing severe clinical manifestations, their effects on milk production in dairy sheep remain controversial. This study aimed to assess the impact of SRLV infections on milk yield and quality in intensively reared dairy sheep. A total of 527 purebred Chios and Lacaune ewes from four infected intensive dairy sheep farms in Greece were randomly selected and their infection status was determined at pre-lambing using both ELISA (serological testing) and real-time PCR (molecular testing). Their milk production was recorded bimonthly for 4 months for one milking period, initiating at the post-weaning, and physicochemical analyses (fat, protein, lactose, solids-not-fat) and estimation of SCC were performed in individual milk samples. Mixed linear regression models were generated for the assessment of the effects of serological, molecular, and infection status (consideration of both serological and molecular status) on milk production. Prevalence rates of SRLV infections exceeded 60 % in all farms. While serological status had no significant association, molecular status revealed a 10 % reduction in milk yield among infected ewes. Infected seronegative ewes had approximately 20 % less milk, protein, lactose and solids-not-fat yields, and 12 % less fat yield compared to the uninfected ones and approximately 15 % decreased milk, protein, lactose, and solids-not-fat yields, and 7 % decreased fat yield compared to the infected seropositive ones. Our findings underpin the significance of prospective study design and molecular diagnosis to efficiently elucidate the impact of SRLV infections on sheep productivity.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"243 ","pages":"Article 107424"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143161704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of antioxidants on quality and DNA methylation of frozen Anglo-Nubian goat semen","authors":"Fareeda Suwor,&nbsp;Pramote Paengkoum,&nbsp;Chatsirin Nakharuthai,&nbsp;Satoshi Kubota,&nbsp;Samorn Ponchunchoovong","doi":"10.1016/j.smallrumres.2025.107447","DOIUrl":"10.1016/j.smallrumres.2025.107447","url":null,"abstract":"<div><div>This study investigated the effects of different concentrations of antioxidants (glutathione, cysteine, and vitamin E) on the cryopreservation of Anglo-Nubian goat sperm. The antioxidants were administered in a tris-based extender designed to achieve iso-osmolality with seminal fluid and contained combinations of soybean lecithin (3 %) and glycerol (5 %). Sperm with motility higher than 70 % was pooled and used for cryopreservation. The sperm was diluted with a tris-based extender and different concentrations of each antioxidant were supplemented at different concentrations (1, 3, and 5 mM for glutathione, 6, 9, and 12 mM for cysteine, and 1, 2, and 3 mM for vitamin E). A sperm sample without antioxidants was also examined, and Andromed was used as a control. Straws were frozen in liquid nitrogen vapor. After storage for 10 days, the frozen straws were thawed at 37 ºC in a water bath for 30 s and evaluated for sperm motility, viability, acrosome integrity, membrane integrity, mitochondrial activity, lipid peroxidation, and DNA methylation. The results showed that the supplementation of 5 mM glutathione in iso-osmolality of the tris-based semen extender improved plasma membrane damage and increased the pregnancy rate compared to the control group (<em>P</em> &lt; 0.05). Furthermore, adding 5 mM glutathione or 6 mM cysteine to the semen extender decreased the lipid peroxidation of the thawed semen (2.76 and 2.80 nmol/mL, respectively). These results were significantly better than those of the other groups (<em>P</em> &lt; 0.05). Nevertheless, an increased concentration of 3 mM of vitamin E in the semen extender increased the MDA concentration (3.78 nmol/mL) in comparison to those obtained with concentrations of 1 mM (2.99 nmol/mL) and 2 mM (3.31 nmol/mL) of vitamin E (<em>P</em> &lt; 0.05). The highest motility rate was 56.10 ± 0.30 % with a viability rate of 58.00 ± 0.45 %, which was achieved with 5 mM glutathione. These results were significantly higher than those of the other treatments (<em>P</em> &lt; 0.05) but lower than those of the control group, which had a motility rate of 68.70 ± 0.470 % and a viability rate of 67.30 ± 0.56 %. In addition, the supplementation of 5 mM glutathione and 6 mM of cysteine did not show a significant difference in sperm DNA methylation compared to fresh sperm (<em>P</em> &gt; 0.05). In summary, this study demonstrated that the addition of 5 mM glutathione to the diluent with iso-osmolality of Anglo-Nubian goat semen has a good effect on frozen semen quality.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"244 ","pages":"Article 107447"},"PeriodicalIF":1.6,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between attributes extracted from carcass image: Prediction of carcass tissue characteristics and meat quality of hair sheep lambs","authors":"Carolina Sarmanho Freitas ,&nbsp;Alinne Andrade Pereira ,&nbsp;Samanta do Nascimento Monteiro ,&nbsp;Gabriel Xavier Serrão ,&nbsp;Jonas Carneiro Araújo ,&nbsp;Manuela Paula de Mesquita Nunes ,&nbsp;Hugo Andrey Santos dos Santos ,&nbsp;Thomaz Cyro Guimarães de Carvalho Rodrigues ,&nbsp;Jamile Andréa Rodrigues da Silva ,&nbsp;Luciara Celi Chaves Daher ,&nbsp;André Guimarães Maciel e Silva ,&nbsp;Welligton Conceição da Silva ,&nbsp;Andréia Santana Bezerra da Silva ,&nbsp;José de Brito Lourenço-Júnior","doi":"10.1016/j.smallrumres.2025.107448","DOIUrl":"10.1016/j.smallrumres.2025.107448","url":null,"abstract":"<div><div>The objective of this work was to propose a methodology for extracting characteristics from shape and texture descriptors of hair lambs carcasses, obtained by digital image processing. Information from 88 cold carcasses of castrated male lambs was divided into three different databases by the views of the images considered and their respective delimitations, with the purpose of evaluating the explanatory quality of these types of captures, as follows: DB1 – “dorsal view database”, aggregating the dorsal view delimitations (dorsal whole carcass + carcass transverse segmentation in dorsal view) with 64 Manifest Variables (MVs) in the IMAGE Latent Variable (LV); DB2 – “sideview database”, with the side view delimitations (sidewhole carcass + carcass transverse segmentation in side view) and 64 MV's in the IMAGE LV; and DB3 – “complete database”, accounting for all dorsal and side delimitations and totaling 128 MV's in IMAGE LV. Regarding the validation of the structural model, referring to the part of the model that evaluates the relationships between the LVs, only the DB1 III and DB3 III models met all the necessary requirements. The PLS-PM was effective in explaining the causal relationships between the variables studied. All models studied obtained sufficient performance to validate the measurement model, with models DB1 and DB2 obtaining better predictive and explanatory performance, with the set of characteristics obtained from the image of the lateral carcass, plus the weight of the cold carcass and the carcass scores, latent variables PRIMAL CUTS and QUALITY.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"244 ","pages":"Article 107448"},"PeriodicalIF":1.6,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing anthelmintic resistance on small ruminant farms in a tropical production system","authors":"Gurdeep Kour ,&nbsp;Tiago Alves Correa Carvalho da Silva ,&nbsp;Stephen W. Walkden-Brown ,&nbsp;Alice Baleiverata ,&nbsp;Shayna Mala ,&nbsp;Ritesh Rao ,&nbsp;Divesh Prasad ,&nbsp;Frances C. Cowley","doi":"10.1016/j.smallrumres.2025.107446","DOIUrl":"10.1016/j.smallrumres.2025.107446","url":null,"abstract":"<div><div>Imidazothiazoles and benzimidazole are the only classes of anthelmintic drugs that have been used over the past 40 years in Fiji. Recently, concerns have arisen that anthelminthic resistance could be widespread and affect animal health and productivity in Fiji. The present study was designed to evaluate the current anthelmintic resistance status in Fiji's small ruminant farms. The study included 11 farms from the two (Western and Northern divisions) most relevant areas of small ruminant production in Fiji. The anthelmintic treatments tested were levamisole (LEV), albendazole (ALB), levamisole + albendazole combination (LEV+ALB), ivermectin (IVM), moxidectin (MOX), closantel (CLO) and a negative control (CON). The anthelmintic’s efficacy was tested using faecal egg count reduction (FECR) tests and copro-cultures on days 14, 28, and 42 after treatment administration. The lowest mean FECR on day 14 was observed for ALB (65.2 %) followed by LEV (91.6 %), ALB + LEV (94.3 %), IVM (97.4 %) and MOX (98.8 %). The most relevant genera of GIN encountered were <em>Haemonchus</em> and <em>Trichostrongylus</em> spp., with no distinct pattern of resistance to drug groups between the two populations. None of the tested drugs (MOX and CLO) presented FECR over 95 % on days 28 and 42. Overall, the level of anthelmintic resistance observed was lower than hypothesised for this study. The combination of LEV+ALB has proven effective and presents an appealing treatment option for managing anthelmintic resistance and worm burden reduction in Fiji.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"244 ","pages":"Article 107446"},"PeriodicalIF":1.6,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “A randomized control trial investigating the effectiveness of a commercial pneumonia vaccine (Part II): Weaned lambs” [Small Rumin. Sci. 234 (2024) 107269]","authors":"M.D. Gardner ,&nbsp;J. Van Donkersgoed ,&nbsp;M.T. Spinato ,&nbsp;C.A. Bauman","doi":"10.1016/j.smallrumres.2025.107440","DOIUrl":"10.1016/j.smallrumres.2025.107440","url":null,"abstract":"","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"245 ","pages":"Article 107440"},"PeriodicalIF":1.6,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143601025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of probiotic supplementation in Kamieniec lambs on meat quality","authors":"Tomasz Daszkiewicz ,&nbsp;Jan Miciński ,&nbsp;Roman Wójcik ,&nbsp;Dawid Tobolski ,&nbsp;Grzegorz Zwierzchowski ,&nbsp;Tulegen Kobzhassarov ,&nbsp;Katarzyna Ząbek ,&nbsp;Klaudia Charkiewicz","doi":"10.1016/j.smallrumres.2025.107444","DOIUrl":"10.1016/j.smallrumres.2025.107444","url":null,"abstract":"<div><div>The aim of this study was to evaluate changes in the quality of the <em>Longissimus thoracis et lumborum</em> (LTL) muscle in young suckling rams of the Kamieniec breed administered a probiotic preparation. Beginning at 11 days of age, lambs in the experimental group received an aqueous solution of the tested probiotic (<em>Lactobacillus plantarum</em> - 2 serotypes, <em>Bifidobacterium animalis</em>, <em>Lactobacillus raminosus</em>) <em>per os</em> for 30 days. The dose of the probiotic was adjusted for lamb age: 11–20 days - 1 g, 21–30 days - 2 g, 31–40 days - 3 g. The viable count per g of the probiotic was 10⁹ CFU. At the end of the experiment, lambs of the control (C, n = 6) and experimental (E, n = 6) groups were slaughtered, and samples of their LTL muscles were subjected to analyses. In group E, the LTL muscle had a lower (<em>P</em> ≤ 0.05) pH value, and a lower (<em>P</em> ≤ 0.05) contribution of yellowness (b*) to the color. A sensory analysis of lamb meat in group E revealed its lower (<em>P</em> ≤ 0.05) juiciness, and a tendency (<em>P</em> &gt; 0.05) towards higher desirability of aroma and taste, lower intensity of these attributes, and higher tenderness. In group E, intramuscular fat (IMF) was characterized by higher (<em>P</em> ≤ 0.05) proportions of fatty acid C18:1 cis9 and total MUFAs, a higher (<em>P</em> ≤ 0.05) MUFA/SFA ratio, and higher (<em>P</em> ≤ 0.05) nutritional value. In group C, IMF had higher (<em>P</em> ≤ 0.05) concentrations of fatty acids C18:3 and C20:1. The results of this study indicate that 30-day supplementation with the tested probiotic improves meat quality in very young lambs of the Kamieniec breed.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"244 ","pages":"Article 107444"},"PeriodicalIF":1.6,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143094966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The pharmacokinetics of sulpiride and its effect on sexual behaviours and LH concentrations in anestrous does (Capra hircus)","authors":"Murat Yuksel ,&nbsp;Orhan Corum ,&nbsp;Mehmet Bugra Kivrak ,&nbsp;Duygu Durna Corum ,&nbsp;Erdinc Turk ,&nbsp;Abdurrahman Takci ,&nbsp;Sara Busra Yardımci ,&nbsp;Mehmet Akif Demirel ,&nbsp;Kamil Uney","doi":"10.1016/j.smallrumres.2025.107442","DOIUrl":"10.1016/j.smallrumres.2025.107442","url":null,"abstract":"<div><div>The aim of this study was to investigate the pharmacokinetics of sulpiride and its effect on sexual behaviours and LH concentrations in anestrous does (<em>Capra hircus</em>). This study was carried out in two stages: pharmacokinetics (stage I) and effect on LH pulsatility, concentration, and estrus display (stage II). In the stage I, sulpiride was administered via intravenous (IV), intramuscular (IM), subcutaneous (SC) and oral routes to does at a dose of 0.6 mg/kg. In the stage II, sulpiride was administered intramuscularly at a dose of 0.6 mg/kg every 12 hours for 10 days. Plasma concentrations of sulpiride were measured using HPLC and pharmacokinetic parameters were calculated by non-compartmental analysis. LH concentrations were quantified using ELISA. The terminal elimination half-life, apparent volume of distribution, and total body clearance of sulpiride following IV administration were 1.76 h, 0.38 L/kg, and 0.15 L/h/kg, respectively. The peak plasma concentration of IM and SC administration was 1.39 and 0.83 μg/mL at 0.53 and 0.78 h, respectively. The bioavailability of sulpiride was 103.30 % for the IM route and 72.21 % for the SC route. Sulpiride showed erratic and low absorption after oral administration. While LH concentrations decreased significantly after sulpiride administration, the LH plus frequency increased significantly. In conclusion, sulpiride with distinctive effect on LH pulse frequency has the potential to be used in protocols for hastening cyclicity. However, more studies are needed on the use of sulpiride in estrus induction protocols.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"244 ","pages":"Article 107442"},"PeriodicalIF":1.6,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic estimation of fat traits in sheep and association of its NR1H4 gene polymorphisms with tail fat deposition","authors":"Kunchao Han ,&nbsp;Fadi Li ,&nbsp;Xiaoxue Zhang ,&nbsp;Huibin Tian ,&nbsp;Deyin Zhang ,&nbsp;Kai Huang ,&nbsp;Yuan zhao ,&nbsp;Yukun Zhang ,&nbsp;Liming Zhao ,&nbsp;Dan Xu ,&nbsp;Jiangbo Cheng ,&nbsp;Zongwu Ma ,&nbsp;Xiaobin Yang ,&nbsp;Mengru Pu ,&nbsp;Weimin Wang","doi":"10.1016/j.smallrumres.2024.107420","DOIUrl":"10.1016/j.smallrumres.2024.107420","url":null,"abstract":"<div><div>Previously sheep tail fat provided warmth and energy, but now excessive fat harms their health and performance, affecting their economic value. This study analysed the correlation between fat traits and their genetic characteristics. As well, the effect of the <em>NR1H4</em> g. 182138945 T &gt; A variant on fat deposition in sheep was investigated. Phenotypic correlations showed significant positive correlations between tail fat, perirenal fat and mesenteric fat weights and carcass fat content. The relative weight of tail fat (relative to either carcass weight or live weight before slaughter) negatively correlates with eye muscle area, while those of the relative weights of perirenal fat and mesenteric fat (relative to either carcass weight or live weight before slaughter) show low positive correlations with eye muscle area. Genetically, intramuscular fat had lower heritability compared to most fat traits, while the carcass fat content had higher heritabilities. A significant positive genetic correlation existed between the tail fat weight, carcass fat content, and intramuscular fat. There was also a significant positive correlation between perirenal fat weight and mesenteric fat weight. Eye muscle area is genetically negatively correlated with the relative weight of tail fat (relative to either carcass weight or live weight before slaughter). The <em>NR1H4</em> g.182138945 T &gt; A association with fat deposition traits showed that the SNP was significantly (<em>P</em> &lt; 0.05) associated with tail fat weight and its relative weight (relative to either carcass weight or live weight before slaughter). The highest levels of <em>NR1H4</em> were expressed in liver, which may affect the deposition of fat in the tail of sheep by regulating energy expenditure. This SNP could be a potential marker for tail fat improvement in sheep.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"242 ","pages":"Article 107420"},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143175985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of early weaning on the development of gastrointestinal tract, rumen microbiota and host gene regulation in goat kids","authors":"Tao Zhong ,&nbsp;Yuqin Wu ,&nbsp;Yaxuan Wang,&nbsp;Aiai Lei,&nbsp;Xinlu Wang,&nbsp;Dunying Hou,&nbsp;Siyuan Zhan,&nbsp;Linjie Wang,&nbsp;Jiaxue Cao,&nbsp;Jiazhong Guo,&nbsp;Li Li,&nbsp;Hongping Zhang,&nbsp;Lili Niu","doi":"10.1016/j.smallrumres.2024.107409","DOIUrl":"10.1016/j.smallrumres.2024.107409","url":null,"abstract":"<div><div>Early weaning (EW) could shorten ewes’ reproductive cycles and promote rumen development in goat kids. During the transition from breastfeeding to a solid diet, significant changes occur in rumen development. However, few studies have focused on goat kids, which are more sensitive to stress than lambs. Here, we assessed the effects of EW on hematological parameters, rumen microbiota, and host gene expression in Chengdu Brown goat kids. The EW kids (weaned at day 42) did not display differences in growth performance and blood biochemical parameters compared to the control group (Control group, CON). However, these younger kids had higher levels of butyric acid than the CON group. EW altered the composition of rumen microbiota and host gene expression, with genes such as <em>RNF183, SCD, SAMD8, MME,</em> and <em>TULP1</em> being up-regulated, all of which are related to protein generation or binding. Conversely, <em>ADRA1A</em>, <em>GLI1</em>, <em>GRIA4</em>, and <em>CACNA1B</em> were down-regulated, involving neuroactive ligand-receptor interaction, MAPK, and cAMP signaling pathways. Furthermore, <em>Christensenellaceae_R_7_group</em> and <em>Prevotella</em> showed significant correlations with the expressions of differently expressed genes <em>DMGDH</em> and <em>FABP</em>. These results could be beneficial to understanding the interactions among indicators in goat kids around weaning and facilitating management of weaning practices in intensive production systems.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"242 ","pages":"Article 107409"},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143175986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of breed traceability for autochthonous attappady black goats of South India","authors":"Marykutty Thomas ,&nbsp;Radhika Gopalakrishnan ,&nbsp;Stephy Thomas ,&nbsp;R. Thirupathy Venkattachalapathy ,&nbsp;Aravindakshan Thazhathuveetil","doi":"10.1016/j.smallrumres.2024.107408","DOIUrl":"10.1016/j.smallrumres.2024.107408","url":null,"abstract":"<div><div>Attappady Black goats, a meat-type breed raised by the tribal communities of the Attappady hill ranges in Kerala, India, play a pivotal role in the region’s economy, heavily contributing to the livelihoods of its socially disadvantaged population. This study aimed to develop and validate a set of breed-specific genetic markers to enable the precise differentiation of Attappady Black goats from Malabari goats. Breed genetic traceability for Attappady Black goats was developed by adopting deterministic approach using breed specific markers. High throughput genome-wide SNP genotypic data of reference goat population (24 each from Attappady Black and Malabari) obtained by Illumina Goat SNP50 BeadChip genotyping was used to identify candidate breed specific markers. Among the 605 numbers of putative Attappady breed specific markers identified in the reference population, nine putative Attappady breed specific markers were selected and were validated for breed specificity by converting them into polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP) markers and genotyped in large sample goat population of 447 goats (246 Attappady Black and 201 Malabari). On validation, four markers retained their breed specificity with probability of identification (<em>Pi</em>) ranging from 0.24 to 0.66. Panel of four Attappady Black breed specific markers yielded <em>Pi</em> of 0.92 with a probability of misjudgement (<em>Pm)</em> of 0.04. These breed-specific markers hold significant potential for facilitating breed traceability thus ensuring their conservation and enhancing the socioeconomic status of the tribal communities that rely on them. Future research should focus on the broader application of these markers in routine breed authentication and in marketing niche livestock products.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"242 ","pages":"Article 107408"},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143176002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}