Seminars in nephrology最新文献_第2页

Challenges in Spatial Metabolomics and Proteomics for Functional Tissue Unit and Single-Cell Resolution. 功能性组织单元和单细胞分辨率的空间代谢组学和蛋白质组学的挑战。

IF 2.8 3区医学

Seminars in nephrology Pub Date : 2025-04-21 DOI: 10.1016/j.semnephrol.2025.151583

Kevin J Zemaitis, Ljiliana Paša-Tolić

{"title":"Challenges in Spatial Metabolomics and Proteomics for Functional Tissue Unit and Single-Cell Resolution.","authors":"Kevin J Zemaitis, Ljiliana Paša-Tolić","doi":"10.1016/j.semnephrol.2025.151583","DOIUrl":"https://doi.org/10.1016/j.semnephrol.2025.151583","url":null,"abstract":"In the last decade, advanced developments of mass spectrometry-based assays have made spatial measurements of hundreds of metabolites and thousands of proteins not only possible, but routine. The information obtained from such mass spectrometry imaging experiments traces metabolic events and helps decipher feedback loops across anatomical regions, connecting genetic and metabolic networks that define phenotypes. Herein we overview developments in the field over the past decade, highlighting several case studies demonstrating direct measurement of metabolites, proteins, and proteoforms from thinly sliced tissues at the level of functional tissue units, approaching single-cell levels. Much of this work is feasible due to multidisciplinary team science, and we offer brief perspectives on paths forward and the challenges that persist with adoption and application of these spatial omics techniques at the single-cell level on mammalian kidneys. Data analysis and reanalysis still pose issues that plague spatial omics, but many mass spectrometry imaging platforms are commercially available. With greater harmonization across platforms and rigorous quality control, greater adoption of these platforms will undoubtedly provide major insights in complex diseases. Semin Nephrol 36:x-xx © 20xx Elsevier Inc. All rights reserved.","PeriodicalId":21756,"journal":{"name":"Seminars in nephrology","volume":" ","pages":"151583"},"PeriodicalIF":2.8,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-Omics Integration in Nephrology: Advances, Challenges, and Future Directions. 肾病学中的多组学整合：进展、挑战和未来方向。

IF 2.8 3区医学

Seminars in nephrology Pub Date : 2025-04-11 DOI: 10.1016/j.semnephrol.2025.151584

Afaf Saliba, Yuheng Du, Tianqing Feng, Lana Garmire

引用次数: 0

Overview: Spatial Metabolomics Review Series. 综述：空间代谢组学综述系列。

IF 2.8 3区医学

Seminars in nephrology Pub Date : 2025-04-07 DOI: 10.1016/j.semnephrol.2025.151576

Kumar Sharma, Ravi Iyengar

引用次数: 0

Integrating Metabolomics and Transcriptomics to Characterize Differential Functional Capabilities of Kidney Proximal Tubule Cell Subtypes. 整合代谢组学和转录组学来表征肾近端小管细胞亚型的不同功能能力。

IF 2.8 3区医学

Seminars in nephrology Pub Date : 2025-04-02 DOI: 10.1016/j.semnephrol.2025.151577

Jens Hansen, Mustafa M Siddiq, John Cijiang He, Ravi Iyengar

{"title":"Integrating Metabolomics and Transcriptomics to Characterize Differential Functional Capabilities of Kidney Proximal Tubule Cell Subtypes.","authors":"Jens Hansen, Mustafa M Siddiq, John Cijiang He, Ravi Iyengar","doi":"10.1016/j.semnephrol.2025.151577","DOIUrl":"https://doi.org/10.1016/j.semnephrol.2025.151577","url":null,"abstract":"The coupling between energy metabolism and transport processes is a key feature that defines the functional capability of proximal tubule cells. Recent studies using metabolomics and transcriptomics provide insights into the relationships between changes in single-cell transcriptomic profiles and energy metabolism during kidney development and in disease states. In this review, we describe insights from these studies and how mapping of metabolites to functional pathways within cells enables these insights. We also describe our analyses of fatty acid metabolism pathways from single-cell transcriptomic data obtained by the Kidney Precision Medicine Project, which indicate that proximal tubule cell subtypes can be divided into two major groups with high and low levels of mRNAs for fatty acid (beta) oxidation enzymes. On average, patients with CKD have higher levels of cells with low fatty acid oxidation capability. These cells also have lower levels of sodium transporters. Within each group of proximal tubule cell subtypes there is considerable variability between individual patients. Integrating these data with metabolomics analyses can provide insights into how the differential metabolic capabilities of proximal tubule cells are related to disease features in individual patients. Identifying such relationships can lead to development of precision medicine approaches in nephrology.","PeriodicalId":21756,"journal":{"name":"Seminars in nephrology","volume":" ","pages":"151577"},"PeriodicalIF":2.8,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of Mucosal Immunity: What Can We Learn From Animal and Human Studies? 粘膜免疫的作用：我们能从动物和人体研究中学到什么？

IF 2.8 3区医学

Seminars in nephrology Pub Date : 2024-09-01 Epub Date: 2025-03-12 DOI: 10.1016/j.semnephrol.2025.151566

Patrick J Gleeson, Renato C Monteiro

引用次数: 0

IgA Vasculitis and IgA Nephropathy: Two Sides of the Same Coin? IgA血管炎和IgA肾病：同一枚硬币的两面？

IF 2.8 3区医学

Seminars in nephrology Pub Date : 2024-09-01 Epub Date: 2025-03-11 DOI: 10.1016/j.semnephrol.2025.151571

Evangéline Pillebout

{"title":"IgA Vasculitis and IgA Nephropathy: Two Sides of the Same Coin?","authors":"Evangéline Pillebout","doi":"10.1016/j.semnephrol.2025.151571","DOIUrl":"10.1016/j.semnephrol.2025.151571","url":null,"abstract":"IgA vasculitis (IgAV) is considered a systemic form of IgA nephropathy (IgAN). The two diseases share similar geographic and ethnic distribution, along with common variants in genetic association studies. The pathophysiology of IgAN and IgA vasculitis nephritis (IgAVN) can be explained by the four-hit hypothesis. Key molecules involved at each step in both diseases were evaluated as diagnostic and prognostic biomarkers with many common factors, most prominently serum galactose-deficient IgA1. On kidney biopsy, the two diseases are indistinguishable, and the established histological Oxford classification for IgAN will soon be validated for IgAVN. Chronic lesions (segmental glomerulosclerosis and tubular atrophy / interstitial fibrosis) seem more frequent in IgAN, while proliferative lesions (endocapillary hypercellularity and crescents) are more frequent in IgAVN, which could explain the worse IgAN renal prognosis. Due to characteristic skin rash, IgAVN patients are diagnosed precociously. Conversely, the frequent absence of overt clinical signs in IgAN leads to a delayed diagnostic kidney biopsy in the disease evolution, which explains the chronic pathologic lesions. From a therapeutic perspective, while impressive advances have been made in recent years for IgAN, there is a glaring lack of evidence-based guidelines for the treatment of IgAVN. Large therapeutic clinical studies are required, and future IgAN trials should include IgAVN.","PeriodicalId":21756,"journal":{"name":"Seminars in nephrology","volume":" ","pages":"151571"},"PeriodicalIF":2.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143606465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IgA Nephropathy: Epidemiology and Disease Risk Across the World. IgA肾病：全球流行病学和疾病风险。

IF 2.8 3区医学

Seminars in nephrology Pub Date : 2024-09-01 Epub Date: 2025-03-12 DOI: 10.1016/j.semnephrol.2025.151564

Malak Ghaddar, Mark Canney, Sean J Barbour

{"title":"IgA Nephropathy: Epidemiology and Disease Risk Across the World.","authors":"Malak Ghaddar, Mark Canney, Sean J Barbour","doi":"10.1016/j.semnephrol.2025.151564","DOIUrl":"10.1016/j.semnephrol.2025.151564","url":null,"abstract":"Despite decades of research, our knowledge of the global epidemiology of IgA nephropathy remains limited. Much of what we know about IgA nephropathy incidence comes from biopsy registry studies that are subject to bias related to differences in screening programs, referral patterns, and access to healthcare. Fewer epidemiologic studies used an appropriate data infrastructure that includes a well-defined source population. Nonetheless, all these studies show considerable geographic variation in disease incidence with an increase from west to east and south to north across Eurasia. This pattern is partly explained by the distribution of genetic risk alleles in individuals of European and East Asian ancestry. Although historically thought to be an indolent disease, recent long-term follow-up studies have demonstrated an exceptionally high lifetime risk of kidney failure. The International IgA Nephropathy Prediction Tool, derived and validated in multiple ethnically diverse cohorts, has improved our ability to identify patients at high risk of progression who may benefit from therapies being tested in clinical trials. The earlier identification of high-risk patients, evaluation of novel risk factors, and accurate assessment of global disease burden require high-quality regional data infrastructures and broad collaborative efforts to ensure the impact of new treatments is maximized.","PeriodicalId":21756,"journal":{"name":"Seminars in nephrology","volume":" ","pages":"151564"},"PeriodicalIF":2.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Pathology of IgA Nephropathy: How Can It Inform Management? IgA肾病的病理：如何为管理提供信息？

IF 2.8 3区医学

Seminars in nephrology Pub Date : 2024-09-01 Epub Date: 2025-03-10 DOI: 10.1016/j.semnephrol.2025.151568

Mark Haas

{"title":"The Pathology of IgA Nephropathy: How Can It Inform Management?","authors":"Mark Haas","doi":"10.1016/j.semnephrol.2025.151568","DOIUrl":"10.1016/j.semnephrol.2025.151568","url":null,"abstract":"IgA nephropathy (IgAN), the world's most common form of primary glomerulonephritis (GN), has a variable clinical and pathologic presentation. While all cases of IgAN show dominant or codominant glomerular IgA deposits, their histologic appearance can range from essentially normal to severe crescentic GN. Oxford (MEST-C) scoring is widely used to classify IgAN on kidney biopsies and has been validated to correlate with clinical presentation and as an independent predictor of kidney outcomes in multiple studies. Components of MEST-C, most notably endocapillary hypercellularity (E score) and crescents (C score), have also been shown to correlate with response to immunosuppressive therapy. Furthermore, immunohistologic evidence of complement activation by the alternative pathway and sometimes the lectin pathway correlates with histologic lesions, proteinuria, and kidney survival, suggesting the complement cascade as a potential therapeutic target. Recent clinical trials have demonstrated the potential of newer classes of immunosuppressive agents as well as complement inhibitors to reduce proteinuria, a marker associated with disease progression, in patients with IgAN. While pathologic studies of kidney biopsies have generally not been part of these trials, this review presents an algorithm by which kidney biopsy findings can be used to guide the choice of therapeutic agents in patients with IgAN.","PeriodicalId":21756,"journal":{"name":"Seminars in nephrology","volume":" ","pages":"151568"},"PeriodicalIF":2.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143606466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Updates on IgA Nephropathy. IgA肾病的最新进展。

IF 2.8 3区医学

Seminars in nephrology Pub Date : 2024-09-01 Epub Date: 2025-03-12 DOI: 10.1016/j.semnephrol.2025.151574

Dana V Rizk

引用次数: 0

Post-transplant IgA Nephropathy. 移植后IgA肾病。

IF 2.8 3区医学

Seminars in nephrology Pub Date : 2024-09-01 Epub Date: 2025-03-13 DOI: 10.1016/j.semnephrol.2025.151570

Song C Ong, Bruce A Julian

引用次数: 0