Seminars in cell & developmental biology最新文献

{"title":"The impact of antidepressants on human neurodevelopment: Brain organoids as experimental tools","authors":"Luciana Simões Rafagnin Marinho ,&nbsp;Gabrielly Maria Denadai Chiarantin ,&nbsp;Juliane Midori Ikebara ,&nbsp;Débora Sterzeck Cardoso ,&nbsp;Théo Henrique de Lima-Vasconcellos ,&nbsp;Guilherme Shigueto Vilar Higa ,&nbsp;Mariana Sacrini Ayres Ferraz ,&nbsp;Roberto De Pasquale ,&nbsp;Silvia Honda Takada ,&nbsp;Fabio Papes ,&nbsp;Alysson R. Muotri ,&nbsp;Alexandre Hiroaki Kihara","doi":"10.1016/j.semcdb.2022.09.007","DOIUrl":"10.1016/j.semcdb.2022.09.007","url":null,"abstract":"<div><p><span>The use of antidepressants during pregnancy benefits the mother's well-being, but the effects of such substances on neurodevelopment remain poorly understood. Moreover, the consequences of early exposure to antidepressants may not be immediately apparent at birth. </span><em>In utero</em><span><span><span> exposure to selective serotonin reuptake inhibitors<span><span><span> (SSRIs) has been related to developmental abnormalities, including a reduced white matter volume. Several reports have observed an increased incidence of autism spectrum disorder (ASD) and attention deficit hyperactivity disorder (ADHD) after prenatal exposure to SSRIs such as sertraline, the most widely prescribed SSRI. The advent of human-induced pluripotent stem cell (hiPSC) methods and assays now offers appropriate tools to test the consequences of such compounds for neurodevelopment in vitro. In particular, hiPSCs can be used to generate cerebral organoids – self-organized structures that recapitulate the morphology and complex physiology of the developing human brain, overcoming the limitations found in 2D cell culture and experimental </span>animal models for testing </span>drug efficacy and side effects. For example, single-cell </span></span>RNA sequencing (scRNA-seq) and electrophysiological measurements on organoids can be used to evaluate the impact of antidepressants on the </span>transcriptome<span><span><span> and neuronal activity signatures in developing neurons. While the analysis of large-scale </span>transcriptomic data depends on dimensionality reduction methods, </span>electrophysiological recordings rely on temporal data series to discriminate statistical characteristics of neuronal activity, allowing for the rigorous analysis of the effects of antidepressants and other molecules that affect the developing nervous system, especially when applied in combination with relevant human cellular models such as brain organoids.</span></span></p></div>","PeriodicalId":21735,"journal":{"name":"Seminars in cell & developmental biology","volume":"144 ","pages":"Pages 67-76"},"PeriodicalIF":7.3,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9195911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of stem cells in engineered vascular graft and vascularized organs","authors":"Shanlan Zhao ,&nbsp;Qiao Zhang ,&nbsp;Min Liu ,&nbsp;Jiahui Du ,&nbsp;Tingting Wang ,&nbsp;Yanzhao Li ,&nbsp;Wen Zeng","doi":"10.1016/j.semcdb.2022.10.003","DOIUrl":"10.1016/j.semcdb.2022.10.003","url":null,"abstract":"<div><p>Recent studies report that stem cell therapies<span> have been applied successfully to patients, This has increased anticipations that this regeneration strategy could be a potential method to treat a wide range of intractable diseases some day. Stem cells offer new prospects for the treatment of incurable diseases and for tissue regeneration<span> and repairation because of their unique biological properties. Angiogenesis<span> a key process in tissue regeneration and repairation. Vascularization of organs is one of the main challenges hindering the clinical application of engineered tissues. Efficient production of engineered vascular grafts and vascularized organs is of critical importance for regenerative medicine. In this review, we focus on the types of stem cells that are widely used in tissue engineering and regeneration, as well as their application of these stem cells in the construction of tissue-engineered vascular grafts and vascularization of tissue-engineered organs.</span></span></span></p></div>","PeriodicalId":21735,"journal":{"name":"Seminars in cell & developmental biology","volume":"144 ","pages":"Pages 31-40"},"PeriodicalIF":7.3,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9194933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organoids as a novel tool in modelling infectious diseases","authors":"Lucas Felipe de Oliveira ,&nbsp;Daniel Mendes Filho ,&nbsp;Bruno Lemes Marques ,&nbsp;Giovana Figueiredo Maciel ,&nbsp;Ricardo Cambraia Parreira ,&nbsp;José Rodrigues do Carmo Neto ,&nbsp;Priscilla Elias Ferreira Da Silva ,&nbsp;Rhanoica Oliveira Guerra ,&nbsp;Marcos Vinicius da Silva ,&nbsp;Helton da Costa Santiago ,&nbsp;Alexander Birbrair ,&nbsp;Alexandre H. Kihara ,&nbsp;Valdo José Dias da Silva ,&nbsp;Talita Glaser ,&nbsp;Rodrigo R. Resende ,&nbsp;Henning Ulrich","doi":"10.1016/j.semcdb.2022.09.003","DOIUrl":"10.1016/j.semcdb.2022.09.003","url":null,"abstract":"<div><p>Infectious diseases worldwide affect human health and have important societal impacts. A better understanding of infectious diseases is urgently needed. In vitro and in vivo infection models have brought notable contributions to the current knowledge of these diseases. Organoids are multicellular culture systems resembling tissue architecture and function, recapitulating many characteristics of human disease and elucidating mechanisms of host–infectious agent interactions in the respiratory and gastrointestinal systems, the central nervous system and the skin. Here, we discuss the applicability of the organoid technology for modeling pathogenesis, host response and features, which can be explored for the development of preventive and therapeutic treatments.</p></div>","PeriodicalId":21735,"journal":{"name":"Seminars in cell & developmental biology","volume":"144 ","pages":"Pages 87-96"},"PeriodicalIF":7.3,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9194366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulatory mechanisms of stem cell differentiation: Biotechnological applications for neurogenesis","authors":"Bruno L. Marques ,&nbsp;Giovana Figueiredo Maciel ,&nbsp;Marcello R. Brito Júnior ,&nbsp;Lucas D. Dias ,&nbsp;Sérgio Scalzo ,&nbsp;Anderson K. Santos ,&nbsp;Alexandre Hiroaki Kihara ,&nbsp;Helton da Costa Santiago ,&nbsp;Ricardo C. Parreira ,&nbsp;Alexander Birbrair ,&nbsp;Rodrigo R. Resende","doi":"10.1016/j.semcdb.2022.09.014","DOIUrl":"10.1016/j.semcdb.2022.09.014","url":null,"abstract":"<div><p>The world population’s life expectancy is growing, and neurodegenerative disorders common in old age require more efficient therapies. In this context, neural stem cells (NSCs) are imperative for the development and maintenance of the functioning of the nervous system and have broad therapeutic applicability for neurodegenerative diseases. Therefore, knowing all the mechanisms that govern the self-renewal, differentiation, and cell signaling<span><span> of NSC is necessary. This review will address some of these aspects, including the role of growth and transcription factors, epigenetic modulators, </span>microRNAs<span>, and extracellular matrix components. Furthermore, differentiation and transdifferentiation processes will be addressed as therapeutic strategies showing their significance for stem cell-based therapy.</span></span></p></div>","PeriodicalId":21735,"journal":{"name":"Seminars in cell & developmental biology","volume":"144 ","pages":"Pages 11-19"},"PeriodicalIF":7.3,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9194503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfluidic organoids-on-a-chip: The future of human models","authors":"Gloria Saorin ,&nbsp;Isabella Caligiuri ,&nbsp;Flavio Rizzolio","doi":"10.1016/j.semcdb.2022.10.001","DOIUrl":"10.1016/j.semcdb.2022.10.001","url":null,"abstract":"<div><p><span>Microfluidics opened the possibility to model the physiological environment by controlling fluids flows, and therefore nutrients supply. It allows to integrate external stimuli such as electricals or mechanicals and in situ monitoring important parameters such as pH, oxygen and metabolite concentrations. Organoids are self-organized 3D organ-like clusters, which allow to closely model original organ functionalities. Applying microfluidics to organoids allows to generate powerful human models for studying organ development, diseases, and drug testing. In this review, after a brief introduction on microfluidics, organoids and organoids-on-a-chip are described by organs (brain, heart, </span>gastrointestinal tract, liver, pancreas) highlighting the microfluidic approaches since this point of view was overlooked in previously published reviews. Indeed, the review aims to discuss from a different point of view, primary microfluidics, the available literature on organoids-on-a-chip, standing out from the published literature by focusing on each specific organ.</p></div>","PeriodicalId":21735,"journal":{"name":"Seminars in cell & developmental biology","volume":"144 ","pages":"Pages 41-54"},"PeriodicalIF":7.3,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9194510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brain organoids, consciousness, ethics and moral status","authors":"Jacob Jeziorski ,&nbsp;Reuven Brandt ,&nbsp;John H. Evans ,&nbsp;Wendy Campana ,&nbsp;Michael Kalichman ,&nbsp;Evan Thompson ,&nbsp;Lawrence Goldstein ,&nbsp;Christof Koch ,&nbsp;Alysson R. Muotri","doi":"10.1016/j.semcdb.2022.03.020","DOIUrl":"10.1016/j.semcdb.2022.03.020","url":null,"abstract":"<div><p>Advances in the field of human stem cells are often a source of public and ethical controversy. Researchers must frequently balance diverse societal perspectives on questions of morality with the pursuit of medical therapeutics and innovation. Recent developments in brain organoids make this challenge even more acute. Brain organoids are a new class of brain surrogate generated from human pluripotent stem cells (hPSCs). They have gained traction as a model for studying the intricacies of the human brain by using advancements in stem cell biology to recapitulate aspects of the developing human brain <em>in vitro</em><span>. However, recent observation of neural oscillations spontaneously emerging from these organoids raises the question of whether brain organoids are or could become conscious. At the same time, brain organoids offer a potentially unique opportunity to scientifically understand consciousness. To address these issues, experimental biologists, philosophers, and ethicists united to discuss the possibility of consciousness in human brain organoids and the consequent ethical and moral implications.</span></p></div>","PeriodicalId":21735,"journal":{"name":"Seminars in cell & developmental biology","volume":"144 ","pages":"Pages 97-102"},"PeriodicalIF":7.3,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9195442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retinal organoids from human-induced pluripotent stem cells: From studying retinal dystrophies to early diagnosis of Alzheimer’s and Parkinson’s disease","authors":"Marília Inês Móvio ,&nbsp;Théo Henrique de Lima-Vasconcellos ,&nbsp;Gabrieli Bovi dos Santos ,&nbsp;Marcela Bermudez Echeverry ,&nbsp;Elisabetta Colombo ,&nbsp;Leonardo S. Mattos ,&nbsp;Rodrigo Ribeiro Resende ,&nbsp;Alexandre Hiroaki Kihara","doi":"10.1016/j.semcdb.2022.09.011","DOIUrl":"10.1016/j.semcdb.2022.09.011","url":null,"abstract":"<div><p>Human-induced pluripotent stem cells (hiPSCs) have provided new methods to study neurodegenerative diseases. In addition to their wide application in neuronal disorders, hiPSCs technology can also encompass specific conditions, such as inherited retinal dystrophies. The possibility of evaluating alterations related to retinal disorders in 3D organoids increases the truthfulness of <em>in vitro</em><span><span> models. Moreover, both Alzheimer's (AD) and Parkinson’s disease (PD) have been described as causing early retinal alterations, generating beta-amyloid protein accumulation, or affecting dopaminergic </span>amacrine cells. This review addresses recent advances and future perspectives obtained from </span><em>in vitro</em><span> modeling of retinal diseases, focusing on retinitis pigmentosa (RP). Additionally, we depicted the possibility of evaluating changes related to AD and PD in retinal organoids obtained from potential patients long before the onset of the disease, constituting a valuable tool in early diagnosis. With this, we pointed out prospects in the study of retinal dystrophies and early diagnosis of AD and PD.</span></p></div>","PeriodicalId":21735,"journal":{"name":"Seminars in cell & developmental biology","volume":"144 ","pages":"Pages 77-86"},"PeriodicalIF":7.3,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9194376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modelling adult stem cells and their niche in health and disease with epithelial organoids","authors":"Lili Szabó ,&nbsp;Anna C. Seubert ,&nbsp;Kai Kretzschmar","doi":"10.1016/j.semcdb.2022.09.006","DOIUrl":"10.1016/j.semcdb.2022.09.006","url":null,"abstract":"<div><p>Adult stem cells<span> are responsible for homoeostasis<span> and regeneration of epithelial tissues. Stem cell function is regulated by both cell autonomous mechanisms as well as the niche. Deregulated stem cell function contributes to diseases such as cancer. Epithelial organoid cultures generated from tissue-resident adult stem cells have allowed unprecedented insights into the biology of epithelial tissues. The subsequent adaptation of organoid technology enabled the modelling of the communication of stem cells with their cellular and non-cellular niche as well as diseases. Starting from its first model described in 2009, the murine small intestinal organoid, we discuss here how epithelial organoid cultures have been become a prime in vitro research tool for cell and developmental biology, bioengineering, and biomedicine in the last decade.</span></span></p></div>","PeriodicalId":21735,"journal":{"name":"Seminars in cell & developmental biology","volume":"144 ","pages":"Pages 20-30"},"PeriodicalIF":7.3,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9200929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thrombospondin-1 in vascular development, vascular function, and vascular disease","authors":"Bo Liu ,&nbsp;Huan Yang ,&nbsp;Yong-Seok Song ,&nbsp;Christine M. Sorenson ,&nbsp;Nader Sheibani","doi":"10.1016/j.semcdb.2023.07.011","DOIUrl":"10.1016/j.semcdb.2023.07.011","url":null,"abstract":"<div><p>Angiogenesis is vital to developmental, regenerative and repair processes. It is normally regulated by a balanced production of pro- and anti-angiogenic factors. Alterations in this balance under pathological conditions are generally mediated through up-regulation of pro-angiogenic and/or downregulation of anti-angiogenic factors, leading to growth of new and abnormal blood vessels. The pathological manifestation of many diseases including cancer, ocular and vascular diseases are dependent on the growth of these new and abnormal blood vessels. Thrompospondin-1 (TSP1) was the first endogenous angiogenesis inhibitor identified and its anti-angiogenic and anti-inflammatory activities have been the subject of many studies. Studies examining the role TSP1 plays in pathogenesis of various ocular diseases and vascular dysfunctions are limited. Here we will discuss the recent studies focused on delineating the role TSP1 plays in ocular vascular development and homeostasis, and pathophysiology of various ocular and vascular diseases with a significant clinical relevance to human health.</p></div>","PeriodicalId":21735,"journal":{"name":"Seminars in cell & developmental biology","volume":"155 ","pages":"Pages 32-44"},"PeriodicalIF":7.3,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9951001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular cell types as functional units of the efferent vagus nerve","authors":"Tatiana C. Coverdell ,&nbsp;Stephen B.G. Abbott ,&nbsp;John N. Campbell","doi":"10.1016/j.semcdb.2023.07.007","DOIUrl":"10.1016/j.semcdb.2023.07.007","url":null,"abstract":"<div><p><span>The vagus nerve vitally connects the brain and body to coordinate digestive, cardiorespiratory, and immune functions. Its efferent neurons, which project their axons from the brainstem to the viscera, are thought to comprise “functional units” - neuron populations dedicated to the control of specific </span>vagal reflexes<span> or organ functions. Previous research indicates that these functional units differ from one another anatomically, neurochemically, and physiologically but have yet to define their identity in an experimentally tractable way. However, recent work with genetic technology and single-cell genomics suggests that genetically distinct subtypes of neurons may be the functional units of the efferent vagus. Here we review how these approaches are revealing the organizational principles of the efferent vagus in unprecedented detail.</span></p></div>","PeriodicalId":21735,"journal":{"name":"Seminars in cell & developmental biology","volume":"156 ","pages":"Pages 210-218"},"PeriodicalIF":7.3,"publicationDate":"2023-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9888172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}