Scientifica最新文献

{"title":"EGCG and Taxifolin Modulate Secretory Activity and Expression of Dentinogenesis Markers in Odontoblast-Like Cells.","authors":"Cristiane Duque, Rafaela Laruzo Rabelo, Gabriela Pacheco de Almeida Braga, Igor Paulino Mendes Soares, Maria Eduarda de Souza, Daniela Alvim Chrisostomo, Josimeri Hebling, Carlos Alberto de Souza Costa, Anuradha Prakki, Luís Octavio Regasini","doi":"10.1155/sci5/3233536","DOIUrl":"https://doi.org/10.1155/sci5/3233536","url":null,"abstract":"<p><p>Odontoblasts are cells specialized in dentin matrix deposition and the first line of defense when the dentin-pulp complex is injured by pathological processes, such as dental caries and trauma. Natural compounds, such as flavonoids, could be useful to stimulate odontoblast activity and reparative dentinogenesis in vital pulp therapies, especially in immature permanent teeth. This study evaluated the effect of flavonoids on odontoblast secretory activity and the expression of dentinogenesis markers. The effect of flavonoids was evaluated on phenotypic mineralization markers (alkaline phosphatase (ALP) activity and mineralized nodule deposition) by colorimetric assays and on the expression of <i>Alpl</i>, <i>Mmp2</i>, <i>Mmp9</i>, <i>Dmp1</i>, and <i>Dspp</i> genes in odontoblast-like cells by quantitative polymerase chain reaction. Most of the flavonoids did not show toxicity between 100 and 25 μM. In distinct concentrations, epigallocatechin gallate (EGCG), taxifolin, myricetin, quercetin, and kaempferol stimulated the activity of ALP and increased mineralized nodule deposition. However, the highest effect on those phenotypic markers was observed after EGCG and taxifolin treatments. Then, they were selected for evaluation of gene expression. mRNA levels of <i>Dmp1</i> and <i>Dspp</i> highly increased with taxifolin treatment, and <i>Alpl</i> expression was increased for both taxifolin and EGCG groups, without difference between them. <i>Mmp2</i> and <i>Mmp9</i> expression was not affected by these flavonoids. In conclusion, EGCG and taxifolin positively affect phenotypic mineralization markers; in particular, taxifolin highly stimulates early- and late-stage dentinogenesis genes.</p>","PeriodicalId":21726,"journal":{"name":"Scientifica","volume":"2025 ","pages":"3233536"},"PeriodicalIF":2.3,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11986927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144051779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extrarespiratory, Digestive, and Hepatic Manifestations of COVID-19 in a Moroccan Series.","authors":"Hakima Abid, Safae Karim, Nada Lahmidani, Wafae Hammoumi, Aicha Attar, Maryame El Khayari, Abdelilah Benslimane, Maria Lahlali, Asmae Lamine, Dafr Allah Benajah, Sidi Adil Ibrahimi, Mohammed El Abkari, Mohammed El Azami El Idrissi, Ikram Khoussar, Naoual Oubelkacem, Noufissa Alami Drideb, Zineb Khammar, Rhizlane Berrady, Mounia El Yousfi, Bahia Bennani","doi":"10.1155/sci5/3524776","DOIUrl":"https://doi.org/10.1155/sci5/3524776","url":null,"abstract":"<p><p><b>Background:</b> Coronavirus disease 2019 (COVID-19) has emerged as a global human health threat. While SARS-CoV-2 infection exhibits fever and respiratory symptoms, extrarespiratory manifestations were also reported in many cases. <b>Objectives:</b> This study aimed to determine the prevalence of digestive and hepatic symptoms at the onset of infection and to assess whether digestive symptoms are associated with severe disease progression. <b>Patients and Methods:</b> Prospective study was conducted during the first COVID-19 wave (from April to October 2020). It included consenting Moroccan patients diagnosed with COVID-19 based on PCR test and chest computed tomography. <b>Results:</b> A total of 211 patients participated in the study. The patients mean age was 42.3 years, with a sex ratio (F/M) of 1.7. Digestive symptoms were present in 28% of cases, with the most common being nausea or vomiting (12.8%), diarrhea (11.4%), abdominal pain (5.2%), and anorexia (16.6%). These symptoms were significantly associated with diabetes and hypertension. Patients with digestive symptoms reported a significantly higher frequency of anosmia and headache. Hepatic manifestations were present in 21.3%, and digestive symptoms were significantly associated with higher prevalence of liver function disturbances, particularly cholestasis. Nearly half of the patients with digestive symptoms (49.2%) experienced moderate COVID-19, with a higher percentage observed (61.8%) among those aged 42 years or older. However, this association was not statistically significant. <b>Conclusion:</b> Healthcare professionals need to recognize the range of gastrointestinal and hepatic symptoms to ensure timely diagnosis and effective patient management.</p>","PeriodicalId":21726,"journal":{"name":"Scientifica","volume":"2025 ","pages":"3524776"},"PeriodicalIF":2.3,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11986959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143983647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial and Temporal Dynamics of Bentho-Demersal Communities From Bottom Trawl Across the Moroccan Mediterranean Coast in Relation to Environmental Conditions: Implications for Fisheries Management.","authors":"Douaa Slimani, Souad Abdellaoui, Najib El Ouamari, Khaoula Kasmi, Rajae Mouedden, Imade Ouebdil, Nassir Kaddouri, Jamal Settih, Mostafa Layachi, Mohamed Fadili, Khalid Chaabane","doi":"10.1155/sci5/5574051","DOIUrl":"10.1155/sci5/5574051","url":null,"abstract":"<p><p>Understanding the temporal and spatial patterns, as well as habitat preferences, of marine communities is crucial for interpreting ecosystem functioning and effectively protecting marine organisms' resources. In this paper, we investigated the changes in marine communities in the Moroccan Mediterranean (20-620 m depth) over both space and time. Using data from trawl scientific surveys conducted by the National Fisheries Research Institute from 2018 to 2021, we conducted a quantitative analysis to (1) assess the seasonal spatial patterns of bentho-demersal communities, including both economically important species and those not directly targeted by fisheries, and (2) explore the interactions between these communities and environmental factors to gain deeper insights into the dynamics of communities structure. We selected several environmental characterizations including depth, sea surface temperature, bottom temperature, salinity, chlorophyll-a, dissolved oxygen, pH, and bottom seabed nature. Depth was the predominant factor responsible for most of the variation observed in both ecological parameters and the composition of bentho-demersal species. The study largely confirms that bentho-demersal communities along the Moroccan Mediterranean coast respond to their environment, displaying a structural pattern shaped by an offshore to inshore environmental gradient, and exhibiting low seasonal variations. These results have significant implications for fisheries management, offering crucial insights into the parameters influencing the distribution of bentho-demersal communities. This is particularly relevant for mixed-species fisheries, just like those operating in the Moroccan Mediterranean trawl fishery.</p>","PeriodicalId":21726,"journal":{"name":"Scientifica","volume":"2025 ","pages":"5574051"},"PeriodicalIF":2.3,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Genetic and Agromorphological Variation in 30 Soybean (<i>Glycine max</i>) Accessions in Northern and Southern Benin.","authors":"Vincent Ezin, Fatimata Bachabi, Florida Corolle Mintodè Dubogan, Wassiou Ahanchede, Gazali B T A Sanni, Mohamed Salim Moussa, Adam Ahanchede","doi":"10.1155/sci5/3449081","DOIUrl":"10.1155/sci5/3449081","url":null,"abstract":"<p><p>Soybean is a highly nutritious and versatile food that is of great importance in world trade. It is a cash crop for farmers in Benin, grown almost everywhere. However, soybean production faces several challenges, including low yields and disruptions due to weather conditions. Improving soybean production could enhance food security for the population and increase farmers' incomes. A study was conducted to assess the genetic and agromorphological variability of 30 soybean accessions. The study was carried out in a three-repeat alpha lattice design at two different sites in the South of Benin (E1) and North (E2). Qualitative and quantitative traits were collected during the experiments. The qualitative characteristics showed great variability, except for cotyledon color and the presence of pubescence and petiole. The results also showed that Accessions TGm-1199, TGX 1910-14, and TGm-1588 had the best yields in E1, whereas TGX 1951-3F, TGX 1910-14F, and TGm-1253 were found with the highest yields in E2. The phenotypic coefficient of variation was higher than the genotypic coefficient of variation for all traits, and heritability in the broad sense ranged from 20% to 100%. The genetic parameters showed that selection programs can be effective for days to flowering, plant height, hundred-seed weight, seed thickness, seed length and width, days to harvest, pod length and width, and grain yield. The high genetic variability observed within soybean accessions indicates that genotypes could be selected and used as a crop improvement tool.</p>","PeriodicalId":21726,"journal":{"name":"Scientifica","volume":"2025 ","pages":"3449081"},"PeriodicalIF":2.3,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Portulaca oleracea</i> L. Methanolic Extract Protects the Brain of Male Rats Against Alzheimer's Disease.","authors":"Haddad A El Rabey, Samar M Rezk, Seham A Mahmoud, Zienab Abdallah, Mennatallah Loutfy, Adel I Alalawy, Yasmene F Alenazi, Heba Sheta, Nadia Bakry","doi":"10.1155/sci5/7701263","DOIUrl":"https://doi.org/10.1155/sci5/7701263","url":null,"abstract":"<p><p>Alzheimer's disease (AD) occurs as a result of a chronic neurodegenerative disorder that is most frequently linked to a decline in cognitive function and memory. Twenty-four male rats were divided into four groups (<i>n</i> = 6); Group I was the negative control, Group II was the AlCl₃-positive control, and Group III and Group IV were treated with 100 mg and 200 mg/kg of <i>Portulaca oleracea</i> methanolic extract, respectively. Aluminum chloride intoxication in Group II increased lipid peroxidation and decreased antioxidant parameters and affected interleukin-6 (IL-6), the tumor necrosis factor-alpha (TNF-α), acetylcholinesterase (AChE), and amyloid beta (Aβ), which lead to the induction of AD through injuring brain cells of AD rats. Treating the AD rats in Group III (GIII) and Group IV (GIV) with <i>P. oleracea</i> ameliorated the altered parameters in the AD rats. It also increased folic acid and vitamin B12 levels. <i>P. oleracea</i> modulated the physiological, biochemical, and histological changes brought on by AlCl₃ intoxication in rats via oxidative stress and inflammatory pathways. The dose of <i>P. oleracea</i> in GIV successfully modified the behavioral changes brought on by AlCl₃ in the AD rats more than that of GIII.</p>","PeriodicalId":21726,"journal":{"name":"Scientifica","volume":"2025 ","pages":"7701263"},"PeriodicalIF":2.3,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11871972/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Variability and Association of Morpho-Agronomic Traits Among Ethiopian Barley (<i>Hordeum vulgare</i> L) Accessions.","authors":"Alemayehu Zewodu, Wassu Mohammed, Eleni Shiferaw","doi":"10.1155/sci5/3957883","DOIUrl":"10.1155/sci5/3957883","url":null,"abstract":"<p><p>Barley is considered to have Ethiopia as its center of diversity, and it is among the most prominent cereal crops cultivated across different agroecology in the country. However, the available germplasm in the country has not been studied much compared to the number of accessions under conservation and the expected diversity of crops in the country. This study was therefore conducted to estimate the phenotypic variability and association of morphoagronomic traits among 49 barley accessions. The experiment was conducted in 2021 using a 7 × 7 simple lattice design. The results of the analysis of variance indicated significant differences among the accessions for all traits. Moreover, with a mean of 4.02 t·ha^-1, the variation in accessions for grain yield ranged from 2.18 to 6.89 t·ha^-1. The phenotypic and genotypic coefficients of variation varied in the range between 7.25% (days to maturity) and 35.18% (weight of kernels per spike) and 6.61% (peduncle length) and 32.25% (weight of kernels per spike), respectively. Broad-sense heritability and genetic advance as a percentage of mean varied from 43.18 (number of fertile tillers) to 92.5% (days to heading) and 11.87% (peduncle length) to 60.99% (weight of kernel per spike), respectively. Grain yield had positive phenotypic and genotypic correlations with the majority of traits. Moreover, the number of spikelets per spike followed by the number of fertile tillers, thousand kernel weight, and number of kernels per spike had strong positive associations with grain yield and they had a direct, positive genotypic effect on grain yield. Consequently, while selecting accessions for high grain yields, these traits should be considered as well. The Euclidian distances of accessions estimated from quantitative traits ranged from 1.07 to 9.24, and the accessions were clustered into six distinct clusters. Clusters V (32.65%), II (26.5%), VI (24.49%), and IV (12.25%) consisted of the largest proportion of accessions, whereas Clusters I and III consisted of one accession each. From principal components' analysis, 79.65% of the variance was explained by three main components with eigenvalues greater than one. Thus, the current findings suggest that there is wide genetic variation among accessions which may be used for crop improvement and the information generated could also be utilized for genetic conservation.</p>","PeriodicalId":21726,"journal":{"name":"Scientifica","volume":"2025 ","pages":"3957883"},"PeriodicalIF":2.3,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11828655/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Xanthine Oxidase Inhibitors Febuxostat and Allopurinol on Kidney Dysfunction and Histological Damage in Two-Kidney, One-Clip (2K1C) Rats.","authors":"Asif Ul Haque Shuvo, Mirza Alimullah, Ishrat Jahan, Kaniz Fatima Mitu, Md Junaeid Rahman, Kazi Akramuddaula, Ferdous Khan, Pritesh Ranjan Dash, Nusrat Subhan, Md Ashraful Alam","doi":"10.1155/sci5/7932075","DOIUrl":"10.1155/sci5/7932075","url":null,"abstract":"<p><p>In chronic kidney disease (CKD), hyperuricemia is a common phenomenon, presumably due to reduced renal clearance of uric acid. This study investigated the effect of xanthine oxidase (XO) inhibitors allopurinol and febuxostat to prevent oxidative stress in the kidney of two-kidney, one-clip (2K1C) rats. In this investigation, 2K1C rats were used as an experimental animal model for kidney dysfunction. 2K1C rats were provided with food and drinking water and received febuxostat at a dose of 10 mg/kg or allopurinol at 100 mg/kg, respectively. After the treatment completion, all rats were sacrificed, and tissue samples were collected. 2K1C rats exhibited increased plasma creatinine, uric acid level, and glomerular injury assessed based on microscopic findings. Both allopurinol and febuxostat significantly normalized creatinine and uric acid levels. Furthermore, 2K1C rats showed increased lipid peroxidation (LPO), nitric oxide (NO), and advanced oxidation protein products (AOPP) alongside decreased superoxide dismutase (SOD) and catalase activity. Again, both drug treatments ameliorated these elevated oxidative stress parameters in 2K1C rats. The antioxidant genes such as Nrf-2, HO-1, and SOD were also restored in the kidneys of 2K1C rats by allopurinol and febuxostat treatment. 2K1C rats also showed increased IL-1β, IL-6, TNF-α, and NF-кB mRNA expression in the kidneys which were normalized by allopurinol and febuxostat treatment. Thus, the data suggest that XO inhibition protects kidney function potentially by restoring antioxidant enzyme function and suppressing inflammation.</p>","PeriodicalId":21726,"journal":{"name":"Scientifica","volume":"2025 ","pages":"7932075"},"PeriodicalIF":2.3,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11779995/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glucosamine Inhibits the Proliferation of Hepatocellular Carcinoma Cells by Eliciting Apoptosis, Autophagy, and the Anti-Warburg Effect.","authors":"Misako Samizu, Kaoruko Iida","doi":"10.1155/sci5/5685884","DOIUrl":"10.1155/sci5/5685884","url":null,"abstract":"<p><p>Although glucosamine (GlcN) exhibits antitumor effects, its mechanism of action remains controversial. Additionally, its impact on hepatocellular carcinoma (HCC) is not well understood. This study aimed to investigate the antitumor effects of GlcN and its underlying mechanism in a mouse HCC cell line, Hepa1-6. GlcN treatment significantly inhibited Hepa1-6 cell proliferation. Gene expression analysis revealed that GlcN upregulated <i>Chop</i> and <i>Bax</i> while downregulating <i>Bcl2</i>, indicating the involvement of endoplasmic reticulum (ER) stress-induced apoptosis in the antiproliferative effects of GlcN. GlcN also increased the expression of <i>FoxO1</i> and <i>FoxO3</i>, known tumor suppressors in various cancers. Furthermore, GlcN treatment elevated the levels of LC3II (an autophagy marker) and AMP-activated protein kinase activity, suggesting intracellular energy shortage. Indeed, GlcN treatment significantly suppressed glycolytic flux, lactate, and ATP production. Supplementing GlcN treatment with a high glucose concentration (20 mM) significantly attenuated its effect. We postulate that GlcN inhibits Hepa1-6 cell growth by inducing ER stress-induced apoptosis and autophagy and by inhibiting aerobic glycolysis (the Warburg effect), a key hallmark of cancer metabolism. Given that glucose transporter 2 (GLUT2), which is abundantly expressed in hepatocytes, has a high affinity for GlcN, these effects may result from GlcN competing with glucose for hepatocyte uptake by GLUT2. Our novel findings have potential implications for HCC treatment.</p>","PeriodicalId":21726,"journal":{"name":"Scientifica","volume":"2025 ","pages":"5685884"},"PeriodicalIF":2.3,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11735062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143011078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking Biochar's Potential: Innovative Strategies for Sustainable Remediation of Heavy Metal Stress in Tobacco Plants.","authors":"Abdul Ghaffar Shar, Leyi Zhang, Anzhi Lu, Munib Ahmad, Muhammad Saqib, Sadam Hussain, Usman Zulfiqar, Pingping Wang, Lixin Zhang, Mehdi Rahimi","doi":"10.1155/sci5/6302968","DOIUrl":"10.1155/sci5/6302968","url":null,"abstract":"<p><p>Tobacco, being a globally cultivated crop, holds significant social and economic importance. Tobacco plants are susceptible to the adverse effects of heavy metals (HMs), particularly cadmium (Cd), which hinders root development, disrupts water balance, and impedes nutrient absorption. Higher concentrations of HMs, especially Cd, naturally accumulate in tobacco leaves due to complex interactions within the plant-soil continuum. The uptake of Cd by plants from the soil is influenced by several factors, including soil type, pH, irrigation water quality, and the chemical composition of the metal involved. Different techniques, such as bioremediation, phytoremediation, and mycoremediation, have been employed to tackle the issue of HMs. The use of biochar offers a practical solution to mitigate this problem. With its large surface area and porous nature, biochar can effectively alleviate HMs contamination. Under biochar application, metal adsorption primarily occurs through physical adsorption, where metal ions are trapped within the pores of the biochar. Additionally, electrostatic attraction, in which negatively charged biochar surfaces attract positively charged metal ions, is another major mechanism of metal remediation facilitated by biochar. In this review, we documented, compiled, and interpreted novel and recent information on HMs stress on tobacco plants and explored biochar's role in alleviating HMs toxicity. By providing a comprehensive review of the persistent threat posed by Cd to tobacco crops and exploring biochar's potential as a remediation measure, this work aims to enhance our understanding of HMs stress in tobacco and contribute to the development of sustainable agricultural practices.</p>","PeriodicalId":21726,"journal":{"name":"Scientifica","volume":"2025 ","pages":"6302968"},"PeriodicalIF":2.3,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143011097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Diversity of Sangihe Nutmeg (<i>Myristica fragrans</i> Houtt.) Based on Morphological and ISSR Markers.","authors":"Tanti Agustina, Abdul Razaq Chasani, Budi Setiadi Daryono, Muhammad Said Rifqi","doi":"10.1155/sci5/5568104","DOIUrl":"https://doi.org/10.1155/sci5/5568104","url":null,"abstract":"<p><p>Sangihe nutmeg is an important crop because of its usefulness in the pharmacology, spices and cosmetics industries. Sangihe is the oldest active subduction zone island in the Indonesia-Philippines region, where frequent tectonic earthquakes and the geographic and reproductive isolation of Sangihe nutmeg occur. This isolation results in adaptation and speciation because of increasing variability. Using morphological and molecular approaches, we aimed to assess the genetic variety of Sangihe nutmeg based on morphological and intersimple sequence repeat (ISSR) markers. In total, 31 morphological characteristics were examined, and molecular data of ISSR markers using five primers were analysed based on numerical taxonomy using the unweighted pair group method with arithmetic mean (UPGMA) and principal component analysis (PCA) methods. Results showed five major groups of Sangihe nutmeg based on seed variation, that is, thick round, thin round, thick oval, thin oval, and twin seeds, whereas the presence of variation in molecular characters was indicated by DNA polymorphisms between 0% and 33.33%. The phenetic relationships within Sangihe nutmeg, based on morphological and ISSR markers, exhibit two groups with different member compositions. The major morphological characteristics influencing the phenetic relationship pattern were tree shape, leaf shape, fruit shape, number of fractions when ripe, fruit size index, number of fruit indentations, indentation, aryl density, seed shell, and number of seeds.</p>","PeriodicalId":21726,"journal":{"name":"Scientifica","volume":"2024 ","pages":"5568104"},"PeriodicalIF":2.3,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}