Scientifica最新文献

{"title":"Exploring TMPRSS2 Drug Target to Combat Influenza and Coronavirus Infection.","authors":"Krishnaprasad Baby, Megh Pravin Vithalkar, Somasish Ghosh Dastidar, Chiranjay Mukhopadhyay, Rania Hamdy, Sameh S M Soliman, Yogendra Nayak","doi":"10.1155/sci5/3687892","DOIUrl":"https://doi.org/10.1155/sci5/3687892","url":null,"abstract":"<p><p>Respiratory viral infections, including influenza and coronaviruses, present significant health risks worldwide. The recent COVID-19 pandemic highlights the urgent need for novel and effective antiviral agents. The host cell protease, transmembrane serine protease 2 (TMPRSS2), facilitates viral pathogenesis by playing a critical role in viral invasion and disease progression. This protease is coexpressed with the viral receptors of angiotensin-converting enzyme 2 (ACE2) for SARS-CoV-2 in the human respiratory tract and plays a significant role in activating viral proteins and spreading. TMPRSS2 activates the coronavirus spike (S) protein and permits membrane fusion and viral entry by cleaving the virus surface glycoproteins. It also activates the hemagglutinin (HA) protein, an enzyme necessary for the spread of influenza virus. TMPRSS2 inhibitors can reduce viral propagation and morbidity by blocking viral entry into respiratory cells and reducing viral spread, inflammation, and disease severity. This review examines the role of TMPRSS2 in viral replication and pathogenicity. It also offers potential avenues to develop targeted antivirals to inhibit TMPRSS2 function, suggesting a possible focus on targeted antiviral development. Ultimately, the review seeks to contribute to improving public health outcomes related to these viral infections.</p>","PeriodicalId":21726,"journal":{"name":"Scientifica","volume":"2025 ","pages":"3687892"},"PeriodicalIF":2.3,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144015778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethnomedicinal Survey of Plants Used for Treatment of Mild COVID-19-Related Symptoms in Gorontalo Province, Indonesia.","authors":"Wiwied Ekasari, Retno Widyowati, Neny Purwitasari, A Mu'thi A Suryadi, Ram K Sahu","doi":"10.1155/sci5/5849854","DOIUrl":"https://doi.org/10.1155/sci5/5849854","url":null,"abstract":"<p><p>This study aims to document plant species used to fight several diseases related to mild COVID-19 symptoms such as cough, colds, fever, dizziness and diarrhoea in Gorontalo Province, eastern Indonesia. Data were collected from 105 local residents using semistructured questionnaires, open interviews and field surveys. Ethnobotanical indices including use reports (UR), relative frequency of citation (RFC) and fidelity level (FL) were used to analyse the data. A total of 82 plant species from 40 families were recorded. <i>Plectranthus scutellarioides</i> (L.) R.Br. is predominantly used to treat cough, <i>Zingiber officinale</i> Roscoe to treat colds, <i>Bryophyllum pinnatum</i> (Lam.) Oken to treat fever, <i>Allium sativum</i> L. to treat dizziness and <i>Psidium guajava</i> L. to treat diarrhoea. The current research reveals that <i>P. guajava</i> L. is the most popular plant species with a high index value (RFC 0.62, FL 62%). Leaves (46.46%) are the most widely used part of the plant for some of these diseases. Boiling (31.70%) is the main method in preparing medicinal plants, most of which are given orally (77.73%). In addition, no serious side effects caused by the consumption of these medicinal plants in the treatment of several diseases related to mild COVID-19 symptoms have been reported. Current studies reveal the wealth and wide use of plant species to manage cough, colds, fever, dizziness and diarrhoea in the study area. Traditional knowledge of medicinal plants used by local residents of Gorontalo Province may help in the treatment of several diseases related to mild COVID-19 symptoms. Further research on preclinical studies needs to be conducted to provide scientific evidence of this result.</p>","PeriodicalId":21726,"journal":{"name":"Scientifica","volume":"2025 ","pages":"5849854"},"PeriodicalIF":2.3,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12055319/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144013601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Robusta Coffee Husk Extract Increases the Number of Fibroblast and Collagen Density in Gingival Rat Periodontitis.","authors":"Nadie Fatimatuzzahro, Rendra C Prasetya, Amandia D P Shita, Nuzulul Hikmah, Hafiedz Maulana, Dwi K Apriyono, Kenanga D Prameiswari, Hanna A Pratiwi","doi":"10.1155/sci5/8952540","DOIUrl":"https://doi.org/10.1155/sci5/8952540","url":null,"abstract":"<p><p><b>Introduction:</b> Periodontitis is an infectious disease of periodontal tissue caused by the bacteria <i>Porphyromonas gingivalis</i> which can trigger the formation of free radicals. Periodontitis can be treated using metronidazole but long-term use can cause negative effects. Natural ingredients such as robusta coffee husk can be used as an alternative treatment for periodontitis because of its anti-inflammatory, antioxidant, and antibacterial benefits. <b>Aim:</b> To determine the effect of robusta coffee husk extract on the number of fibroblasts and collagen density in gingival Wistar rats induced by <i>P. gingivalis</i>. <b>Methods:</b> Robusta coffee husk extract was extracted using the maceration process with 96% ethanol. Thirty male Wistar rats divided into 6 treatment groups: healthy rat, negative control (<i>P. gingivalis</i> + Aquades sterile), positive control (<i>P. gingivalis</i> + metronidazole), and 3 treatment groups of extract concentration (<i>P. gingivalis</i> + 125, 250, and 500 mg/kgBW). <i>P. gingivalis</i> injection was carried out in the buccal area of the left mandibular first molar of mice every 3 days. Robusta coffee husk extract and metronidazole were given by using sondase once a day for 21 days. The gingival specimens were then processed histologically. Hematoxylin and Eosin (H&E) staining was performed to observe the number of fibroblast, and collagen density was observed by Thricome Mallory. The SPSS application was used to examine the data calculation which included the Shapiro-Wilk, Levene, One-way ANOVA, and post hoc LSD tests. <b>Result:</b> Robusta coffee husk extract at doses of 125, 250, and 500 mg/kgBW showed increased fibroblasts and collagen density in Wistar rats induced by <i>P. gingivalis.</i> <b>Conclusion:</b> The most effective dose of robusta coffee husk extract is 250 mg/kgBW, with an average value of fibroblast number in a field of view is 37 cells, and the average of collagen density in the field of view is 163 pixels.</p>","PeriodicalId":21726,"journal":{"name":"Scientifica","volume":"2025 ","pages":"8952540"},"PeriodicalIF":2.3,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12031600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combating Methotrexate Resistance in Cancer Treatment: A Review on Navigating Pathways and Enhancing Its Efficacy With Fat-Soluble Vitamins.","authors":"Muhsina Kakkadath, Disha Naidu, S K Kanthlal, Khan Sharun","doi":"10.1155/sci5/8259470","DOIUrl":"https://doi.org/10.1155/sci5/8259470","url":null,"abstract":"<p><p>Methotrexate (MTX), a potent analogue and antagonist of folic acid, is a first-line treatment for rheumatoid arthritis, IBD and cancer. The development of MTX resistance contributes to the reduced efficacy and development of adverse reactions, forcing clinicians to withdraw treatment early. This drawback requires combinational approaches to combat the resistance and enhance the efficacy and safety of MTX. To provide a brief overview of MTX resistance and strategies to mitigate its aftereffects in cancer therapy, a literature-based search was conducted using keywords such as cancer pathology, MTX mechanism and resistance, S100A4, folate uptake, folate efflux, P-glycoprotein, beta-catenin and anticancer properties of Vitamins A, D, E and K. Investigations encompassing in vitro studies, in vivo studies and clinical trials were reviewed to identify the mechanisms of resistance induced by MTX and the potential benefits of coadministering fat-soluble vitamins with existing anticancer drugs. Derivates of Vitamin A could target cancer stem cells and increase chemotherapy sensitivity in non-small cell lung cancer. Similarly, calcitriol and cytotoxic medications exhibit additive or synergistic effects. Existing research revealed that fat-soluble vitamins can inhibit drug transporters, such as P-glycoprotein, which inhibit drug efflux, improving chemotherapy efficacy in cancer. As personalised medicine continues to evolve, incorporating combination approaches with MTX and fat-soluble vitamins holds promise for enhancing treatment efficacy, which can counteract MTX resistance via multiple pathways and improve the safety profile.</p>","PeriodicalId":21726,"journal":{"name":"Scientifica","volume":"2025 ","pages":"8259470"},"PeriodicalIF":2.3,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12017957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144011221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silver Nanoparticles Synthesized From <i>Centella asiatica</i> Extract and Asiatic Acid for Enhanced Biofilm Eradication of <i>Streptococcus</i> Associated With Oral Diseases.","authors":"Sukanlaya Leejae, Wattana Pelyuntha, Lavanya Goodla, Auemphon Mordmuang","doi":"10.1155/sci5/4867529","DOIUrl":"https://doi.org/10.1155/sci5/4867529","url":null,"abstract":"<p><p>The biofilm-forming ability of <i>Streptococcus</i> species, particularly <i>Streptococcus mutans</i>, is a key factor in the pathogenesis of dental caries and periodontitis. Current treatments often exhibit limitations such as incomplete biofilm eradication and cytotoxicity to host tissues, highlighting the need for innovative and biocompatible therapeutic approaches. Therefore, this study aimed to investigate the potential of <i>Centella asiatica</i> ethanolic extract, its bioactive triterpenoids (asiatic acid and madecassic acid), and silver nanoparticles (AgNPs) synthesized from the extract as an alternative strategy for targeting <i>S. mutans</i> biofilms. The antibacterial and antibiofilm activities of the synthesized AgNPs against <i>Streptococcus</i> species were evaluated, alongside cytotoxicity assessments on human gingival fibroblast (HGF-1) cells using the MTT assay. The synthesized AgNPs exhibited superior antimicrobial efficacy compared to the extract, with significantly lower minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values (62.5/125 µg/mL) against <i>S. mutans</i> ATCC 25175 and <i>S. mitis</i> ATCC 49456, highlighting their potent bactericidal activity. Moreover, the AgNPs achieved rapid biofilm disruption, reducing biofilm biomass by 76% within 12 h at 1/2 × MIC, significantly outperforming the extract and triterpenoids. Scanning electron microscopy further revealed substantial extracellular polymeric substance degradation and biofilm structural disruption upon AgNP treatment, confirming their pronounced antibiofilm efficacy. In addition, the synthesized AgNPs demonstrated favorable biocompatibility, maintaining 68% cell viability in dental fibroblast cells, suggesting an optimal balance between antimicrobial potency and cytotoxicity. The synergistic interaction between AgNPs and <i>C. asiatica</i> phytochemicals significantly enhanced biofilm disruption compared to nonfunctionalized AgNPs. These findings underscore the potential of <i>C. asiatica</i>-based AgNPs as a novel, plant-derived nanotechnological approach for managing oral infections caused by biofilm-forming <i>Streptococcus</i> species. This study not only contributes to the development of alternative antibiofilm strategies but also paves the way for future clinical applications in oral healthcare.</p>","PeriodicalId":21726,"journal":{"name":"Scientifica","volume":"2025 ","pages":"4867529"},"PeriodicalIF":2.3,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12014262/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144028899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ecological and Statistical Evaluation of Genetic Algorithm (GARP), Maximum Entropy Method, and Logistic Regression in Predicting Spatial Distribution of <i>Astragalus</i> sp.","authors":"Amir Ghahremanian, Abbas Ahmadi, Hamid Toranjzar, Javad Varvani, Nourollah Abdi","doi":"10.1155/sci5/4003408","DOIUrl":"10.1155/sci5/4003408","url":null,"abstract":"<p><p>This study aims to evaluate the potential habitat of <i>Astragalus</i> sp. using three different species distribution modeling methods: the maximum entropy (MaxEnt) model, the Genetic Algorithm for Rule-Set Production (GARP), and logistic regression. The primary objective was to identify key environmental factors that influence the spatial distribution of <i>Astragalus</i> sp. in the Savar-Abad basin's rangelands. Vegetation sampling was carried out across diverse vegetation types within the study area, using 2-10 square meter plots to capture a representative sample of plant species distribution. Soil sampling was conducted at varying depths to capture essential soil properties, including physical (clay, gravel, silt, and sand) and chemical factors (organic matter, electrical conductivity, pH, and lime). Soil maps were generated using interpolation techniques to visualize soil variation across the area. The sampling strategy was designed to ensure comprehensive data collection, allowing for robust model training and validation. MaxEnt, which is a presence-only model, outperformed both the GARP and logistic regression models in predicting suitable habitats for <i>Astragalus</i> sp. Results revealed that soil salinity, elevation, and soil acidity significantly influenced species distribution. The findings also suggest that elevation and salinity have the most substantial effects on habitat suitability, while soil texture (clay, silt, and sand) plays a secondary role. These results are valuable for rangeland management, offering insights into areas where <i>Astragalus</i> sp. could thrive or where interventions might be necessary to improve habitat conditions. In terms of management, this study highlights the importance of considering both ecological and environmental factors when planning conservation and restoration activities for rangelands. The ability to predict species distribution can help optimize resource allocation for habitat restoration and enhance biodiversity conservation efforts.</p>","PeriodicalId":21726,"journal":{"name":"Scientifica","volume":"2025 ","pages":"4003408"},"PeriodicalIF":2.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11976046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143812188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cardio Protection and Antiatherosclerotic Effects of <i>Solanum incanum</i> (Lin.) Extracts in Animal Models.","authors":"Stephen Ngigi Mburu, Mathew Piero Ngugi, John K Mwonjoria","doi":"10.1155/sci5/5594777","DOIUrl":"10.1155/sci5/5594777","url":null,"abstract":"<p><p><i>Solanum incanum</i> is a bushy perennial herb which is used for treatment of various ailments in East Africa including stomach pain, toothaches, ulcers, and cardiovascular diseases. However, there is scarcity of data on its safety, and its effect on the physiology of the cardiovascular system. Hence, this study envisaged evaluating the effect of methanol extract of the herb on myocardial action atherosclerotic tendency and safety. Myocardial activity assay involved determination of the heart rate and force of contraction using an isolated rabbit heart in an organ bath and kymograph, while antiatherosclerotic effects were assayed on blood obtained from white Wistar rats fed on high lipid diet where the levels of low-density lipoproteins, high-density lipoproteins and total cholesterol were determined. Assays of the toxic effects of the extract were carried out on Swiss albino mice while qualitative phytochemical analyses were carried out using one-way Anova and <i>Tukey</i> as the <i>post hoc</i> test, a value of (<i>p</i> < 0.05) was taken as the limit of significance. The plant extracts exhibited an increase in the force of contraction of the heart and decrease in heart rate. It also caused significant (<i>p</i> < 0.05) reduction in serum low-density lipoproteins and total cholesterol as well as elevation of HDL but no effect on hematological parameters. Phytochemical analyses showed the presence of alkaloids, glycosides, saponins flavonoids, terpenoids, steroids, and phenolics. Hence, the <i>S. incanum</i> root extract may contain compounds with antiatherosclerotic effects that are cardioprotective and therefore a potential source of novel remedies for the ailment.</p>","PeriodicalId":21726,"journal":{"name":"Scientifica","volume":"2025 ","pages":"5594777"},"PeriodicalIF":2.3,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972130/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of <i>Spirulina</i> Supplementation on Obesity, Hypertension, Hyperglycemia, and Hyperlipidemia: A Systematic Review.","authors":"Muhamad Firdaus, Ahmad Faris Priambodo","doi":"10.1155/sci5/6637793","DOIUrl":"10.1155/sci5/6637793","url":null,"abstract":"<p><p><b>Aims:</b> This systematic review evaluates how <i>Spirulina</i> supplementation impacts human metabolic syndrome. This review encompasses a broad spectrum of <i>Spirulina</i> supplementation studies, including variations in dosage, trial duration, participants, and the subsequent effects on human metabolic syndrome. <b>Methods:</b> The method involves searching for articles from the Scopus and PubMed databases (up to 2023) to identify relevant studies on <i>Spirulina</i> supplementation. The journal database related to the study was examined using the systematic review approach. <b>Results:</b> Out of 527 articles related to <i>Spirulina</i> supplementation, 13 articles were determined to be suitable for human clinical studies. The treatment is performed at 20 mg to 6 g within 17-360 days. <i>Spirulina</i> contains macro- and micronutrients, vitamins, and minerals that are beneficial for health, as well as several bioactives that play a role in improving metabolic syndrome. This seaweed pigment can reduce obesity, body mass index (BMI), hypertension, hyperglycemia, and dyslipidemia. Its tripeptides lower blood pressure while gamma linoleic acid and glycolipids improve lipid profile. The findings show that <i>Spirulina</i> supplementation improves human metabolic syndrome. It included obesity, hypertension, hyperglycemia, and hyperlipidemia. <b>Conclusion:</b> <i>Spirulina</i> supplementation in humans has a positive effect on metabolic syndrome. It is due to phycocyanin, L-isoleucyl-L-glutaminyl-L-proline (Ile-Gln-Pro), gamma-linolenic acid, and the glycolipid H-b2. However, the form of use, dosage, and treatment should be further researched to determine the right amount for each metabolic syndrome problem.</p>","PeriodicalId":21726,"journal":{"name":"Scientifica","volume":"2025 ","pages":"6637793"},"PeriodicalIF":2.3,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11968159/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Antibacterial Activity of Acetic Acid in Comparison With Three Disinfectants Against Bacteria Isolated From Hospital High-Touch Surfaces.","authors":"Ayesha Muazzam, Sidrah Saleem, Hafiz Muhammad Faizan Nadem, Faiz Ul Haq, Ghaniya Ali, Nida Javed","doi":"10.1155/sci5/7598027","DOIUrl":"10.1155/sci5/7598027","url":null,"abstract":"<p><p>Acetic acid, a readily available and less toxic alternative to conventional disinfectants, is widely used for cleaning in household settings. This study evaluates the antibacterial efficacy of acetic acid against bacteria isolated from hospital high-touch surfaces, comparing its performance to commonly used disinfectants, including phenol, sodium hypochlorite, and didecyldimethylammonium chloride (DDAC). A total of 120 samples were collected from high-touch surfaces in specialized patient areas. The antibacterial activity of acetic acid, phenol, sodium hypochlorite, and DDAC was assessed using the standard broth microdilution method against the isolated bacterial strains. From the 120 samples, 140 bacterial isolates were obtained. Acetic acid demonstrated strong antibacterial activity, with mean minimum inhibitory concentrations (MICs) ranging from 0.05 ± 0.00 to 0.25 ± 0.06 μL/mL, effectively inhibiting coagulase-negative <i>Staphylococcus</i> (CONS), <i>Klebsiella pneumoniae</i>, <i>Proteus vulgaris</i>, <i>Enterococcus</i> species, and <i>Serratia marcescens</i>. Its performance surpassed phenol and DDAC against these strains. Phenol exhibited higher MICs (0.50 ± 0.00 to 0.83 ± 0.10 μL/mL), indicating lower efficacy, while DDAC (0.06 ± 0.00 to 0.17 ± 0.04 μL/mL) and sodium hypochlorite (0.06 ± 0.00 to 0.10 ± 0.00 μL/mL) demonstrated comparable antibacterial effects. Phenol and sodium hypochlorite were found nonsignificant, while DDAC is highly effective at a concentration of 8.5%. Hospital surfaces were found to be contaminated with diverse bacterial strains. Acetic acid demonstrated significant antibacterial efficacy against both gram-positive and gram-negative bacteria, with MICs ranging from 0.05 ± 0.00 to 0.25 ± 0.06 μL/mL, suggesting its potential as an effective, economical, and less toxic alternative to conventional disinfectants.</p>","PeriodicalId":21726,"journal":{"name":"Scientifica","volume":"2025 ","pages":"7598027"},"PeriodicalIF":2.3,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11964715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143773319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Antibiotic Resistance Genes in the Interior Bay of Puno-Peru, Lake Titicaca.","authors":"Pompeyo Ferro, Jhordan Rossel, Ana Lucia Ferro-Gonzales, Eli Morales-Rojas, Euclides Ticona, Romel Guevara, Lizbeth Córdova","doi":"10.1155/sci5/5571355","DOIUrl":"10.1155/sci5/5571355","url":null,"abstract":"<p><p>Water can serve as a source of genetic resistance and act as an amplifier and/or reservoir for genes acquired by human pathogens, which can be released into the environment as pollutants. The interior bay of Puno, part of Lake Titicaca, is a popular tourist attraction, being an active component of the dynamics of the city of Puno. Therefore, the determination of the presence of antibiotic resistance genes (ARGs) in water samples from the interior bay of Puno of six collection points was the main objective of this research work. DNA extraction was conducted, followed by the identification and quantification of 16S rRNA and <i>Escherichia coli uidA</i> gene, two ARGs (<i>bla</i> _TEM and qacEΔ1), and class 1 integron-integrase gene (<i>intI1</i>) by means of quantitative PCR. The <i>intI1</i> and qacEΔ1 genes were detected throughout the interior bay of Puno; however, the abundance of the <i>bla</i> _TEM gene was comparatively lower. The uidA gene was reported only in some sampled points with < LOQ. These findings should raise concerns regarding the potential risk of their dissemination in Lake Titicaca and their impact on public health.</p>","PeriodicalId":21726,"journal":{"name":"Scientifica","volume":"2025 ","pages":"5571355"},"PeriodicalIF":2.3,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11952920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}