Serbian Journal of Experimental and Clinical Research最新文献_第4页

Pharmacological Treatment of Chronic Congestive Heart Failure 慢性充血性心力衰竭的药理学治疗

Serbian Journal of Experimental and Clinical Research Pub Date : 2022-05-16 DOI: 10.2478/sjecr-2021-0011

Mina Poskurica, Đorđe Stevanović, M. Poskurica, M. Petrovic

引用次数: 0

Antitumor Effect of the Synthesized Chalcone Analogues on HeLa Cell Line 合成查尔酮类似物对HeLa细胞的抗肿瘤作用

Serbian Journal of Experimental and Clinical Research Pub Date : 2022-04-18 DOI: 10.2478/sjecr-2021-0065

Marija Anđelković, I. Nikolic, Jovan Luković, M. Mitrovic, I. Zelen, Jovana Muskinja, Z. Ratković, S. Popovic, S. Stankovic, M. S. Pirkovic

{"title":"Antitumor Effect of the Synthesized Chalcone Analogues on HeLa Cell Line","authors":"Marija Anđelković, I. Nikolic, Jovan Luković, M. Mitrovic, I. Zelen, Jovana Muskinja, Z. Ratković, S. Popovic, S. Stankovic, M. S. Pirkovic","doi":"10.2478/sjecr-2021-0065","DOIUrl":"https://doi.org/10.2478/sjecr-2021-0065","url":null,"abstract":"Abstract Chalcones represent a type of flavonoids which are located at vegetative and reproductive organs of plants and they can be metabolic progenitor molecules for several flavonoids and isoflavonoids. Many studies indicated that molecular structure of chalcone accountable for their anti-tumor, anti-inflammatory and anti-oxidant effects. The aim of our research was to investigate anti-tumor effect and mechanism of action of three synthesized chalcone analogues on HeLa cells. The anti-tumor effectiveness of chalcone analogues was compared to effects of the dehydrozingerone and cisplatin that were used as referent substances. The viability of the treated cells was evaluated using MTT assay. Evaluation of cell death was determined by flow cytometry and cells were stained with Annexin V-FITC/7-AAD. The result of our research indicated that used chalcones have stronger antitumor effect relative to the dehydrozingerone and cisplatin. The IC50 values of the chalcones ranged between 1.69-6.18 μM, with CH1 being more cytotoxic after 24 h of treatment, while CH3 being more cytotoxic after 48 h of treatment on HeLa cells. All investigated chalcones induced apoptosis in HeLa cells via mitochondrial pathway, which was detected expression Bax and Bcl- 2 proteins. Our results provided evidence that chalcones induced apoptosis in HeLa cervical carcinoma through the intrinsic apoptotic pathway. These findings provide insights into the molecular mechanism of chalcones-induced cell death.","PeriodicalId":21582,"journal":{"name":"Serbian Journal of Experimental and Clinical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45297993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Antitumor Activity of Palladium(II) Complexes on DU-145 Cell Line in Vitro 钯（II）配合物对DU-145细胞株的体外抗肿瘤活性

Serbian Journal of Experimental and Clinical Research Pub Date : 2022-04-18 DOI: 10.2478/sjecr-2022-0003

D. Simić, M. Zarić, Stefan Jakovljević, Katarina Pantić, Djordje Petrovic, S. Milić, P. Čanović

{"title":"Antitumor Activity of Palladium(II) Complexes on DU-145 Cell Line in Vitro","authors":"D. Simić, M. Zarić, Stefan Jakovljević, Katarina Pantić, Djordje Petrovic, S. Milić, P. Čanović","doi":"10.2478/sjecr-2022-0003","DOIUrl":"https://doi.org/10.2478/sjecr-2022-0003","url":null,"abstract":"Abstract In the area of non-platinum complexes, various complexes containing gold, copper, ruthenium, and palladium have shown a strong cytotoxic effect on different cancer cell lines. The aim of our study was to examine the cytotoxicity of the Pd(II) complexes (C1-C5) and the corresponding ligands (L1-L5) on the DU-145 prostate cancer cell line. Also, due to its clinical application, the cytotoxicity of cisplatin has been examined. Our findings showed that C1- C5 complexes and cisplatin show dose-dependent and strong cytotoxic effects against the DU-145 cell line in vitro. Furthermore, the results demonstrated that early apoptosis was induced by all five Pd(II) complexes. Also, the results showed that complexes C1, C3, and C5 induced G0/G1 phase arrest on DU- 145 cells. Pd(II) complex C2 induced S phase arrest, while C4 complex induced G2/M phase arrest on cancer cells. Additionally, all tested complexes significantly reduced the amount of antiapoptotic protein Bcl-2. Also, there was a significant increase in the concentration of proapoptotic Bax protein in DU-145 cells treated C1-C5 complexes. The results of our research demonstrated that Pd(II) complexes induced apoptosis via the mitochondrial pathway. Thus, it is crucial to further investigate the cytotoxicity of these Pd(II) complexes in vivo. Complex C2 might be a good candidate for a new generation of anticancer drugs.","PeriodicalId":21582,"journal":{"name":"Serbian Journal of Experimental and Clinical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43169040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vitamin D Deficiency in Patients Receiving Antidepressant Medications 服用抗抑郁药物的患者维生素D缺乏

Serbian Journal of Experimental and Clinical Research Pub Date : 2022-04-18 DOI: 10.2478/sjecr-2022-0001

S. Ristić, B. Bajić, Svetlana Radević, S. Subotić, S. Kocić, Katarina M. Janicijevic, A. Arnaut, K. Taušanović

引用次数: 0

Methods for Determination of Meropenem Concentration in Biological Samples 生物样品中美罗培南浓度的测定方法

Serbian Journal of Experimental and Clinical Research Pub Date : 2022-04-11 DOI: 10.2478/sjecr-2022-0005

Aleksandar Rančić

引用次数: 0

Factors Associated with Death of Covid-19 Patients: Case Series 与Covid-19患者死亡相关的因素:病例系列

Serbian Journal of Experimental and Clinical Research Pub Date : 2022-04-11 DOI: 10.2478/sjecr-2021-0036

Z. Djordjevic, J. Gavrilović, R. Suša, A. Pejčić, Biljana Popovska-Jovičić, Predrag Čanović, Radica Živković-Zarić, Miloš N. Milosavljević, V. Opančina, Ivana Petrović, I. Čekerevac, Z. Lazic, M. Folic, Ljiljana Novkovic, S. Jankovic

{"title":"Factors Associated with Death of Covid-19 Patients: Case Series","authors":"Z. Djordjevic, J. Gavrilović, R. Suša, A. Pejčić, Biljana Popovska-Jovičić, Predrag Čanović, Radica Živković-Zarić, Miloš N. Milosavljević, V. Opančina, Ivana Petrović, I. Čekerevac, Z. Lazic, M. Folic, Ljiljana Novkovic, S. Jankovic","doi":"10.2478/sjecr-2021-0036","DOIUrl":"https://doi.org/10.2478/sjecr-2021-0036","url":null,"abstract":"Abstract This aim of this study was to identify potential factors associated with survival in patients with coronavirus disease 2019 (COVID- 19). This study was designed as a case series. It included patients hospitalized in Clinical Centre Kragujevac between March 10, 2020, and June 15, 2020, due to COVID-19. Variables with significant influence on the cure of patients were identified by multivariate logistic regression. A total of 234 patients were included. The average age of the patients was 57.2 ± 15.8 years. Thirty-five patients died (15.0%) and 199 (85.0%) were discharged as completely cured and with the sustained virological response. The study variables with significant influence (expressed as odds ratio – OR) on cure of COVID-19 patients after adjustment for effects of other variables were: stay in an intensive care unit – ICU (OR = 0.007; 95% confidence interval – CI 0.001 – 0.086; p = 0.000), previous hospitalization (OR = 7.802; 95% CI 1.198 – 50.924; p = 0.032), increased body temperature on admission (OR = 0.004; 95% CI 0.000 – 0.771; p = 0.040), higher score of the Charlson Comorbidity Index (OR = 0.428; 95% CI 0.235 – 0.780; p = 0.006), and increased values of C-reactive protein (CRP) serum level (OR = 0.978; 95% CI 0.966 – 0.990; p = 0.000). In conclusion, clinicians should pay attention to patients with high body temperature at admission, presence of multiple comorbidities, high CRP, and patients who stay in an ICU, considering that they could be at risk for fatal outcome.","PeriodicalId":21582,"journal":{"name":"Serbian Journal of Experimental and Clinical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47038339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological Characteristics of Maxillary Molars Interradicular Septum and Clinical Implications -What Do We Know So Far? 上颌磨牙根间隔的形态特征及其临床意义——目前所知的情况?

Serbian Journal of Experimental and Clinical Research Pub Date : 2022-04-05 DOI: 10.2478/sjecr-2022-0014

Zlata Rajkovic Pavlovic, M. Petrovic

{"title":"Morphological Characteristics of Maxillary Molars Interradicular Septum and Clinical Implications -What Do We Know So Far?","authors":"Zlata Rajkovic Pavlovic, M. Petrovic","doi":"10.2478/sjecr-2022-0014","DOIUrl":"https://doi.org/10.2478/sjecr-2022-0014","url":null,"abstract":"Abstract The interradicular septum describes area in the root furcation that separate alveoli of multi-rooted teeth. The shape and dimension depend of the topography of the extraction socket, the geometry of residual root and anatomy of molars alveoli. The clinical implications of this anatomic structure may be used in oral surgery resection procedures, periodontology and implantology. There is an opinion from the surgical and prosthodontic side that center of interradicular septum may be adequate place for immediate implantation. The aim of this study was to investigate morphological characteristics and clinical implications of upper molars interradicular septum. The studies showed that interradicular septum is important for success of resection surgery procedures: hemi-section, bisection and root amputation. It’s reported that furcation involvement in periodontal disease is clinical sign for severe bone loss. By reviewing the available literature for immediate implantation, it’s reported the clinical implications for diagnostical plan (pre-intervention), presurgical (pre-instrumentation) and intraoperative evaluation of interradicular septum for immediate implantation. The cone beam computed tomography analyze study presented mean values of septum height and wide for hypothetical plan of immediate implant placement. The presurgical studies evaluate how to assure preservation of septum and safe implant position in center of septum. The research showed varies therapy modalities that can be used, and how to choose right therapy according the socket type and initial septum width classification. According to observation of studies results, other researches should be considered for CBCT anatomical structure analyze and measures of interradicular septum for immediate implantation planning.","PeriodicalId":21582,"journal":{"name":"Serbian Journal of Experimental and Clinical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47269702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Plasma Exchange in the Treatment of Relapsing Multiple Sclerosis: Case Report 血浆置换治疗复发性多发性硬化1例

Serbian Journal of Experimental and Clinical Research Pub Date : 2022-04-05 DOI: 10.2478/sjecr-2021-0054

M. Vranes, A. Antonijević, Zivanka Djurovic, Maja Savić

{"title":"Plasma Exchange in the Treatment of Relapsing Multiple Sclerosis: Case Report","authors":"M. Vranes, A. Antonijević, Zivanka Djurovic, Maja Savić","doi":"10.2478/sjecr-2021-0054","DOIUrl":"https://doi.org/10.2478/sjecr-2021-0054","url":null,"abstract":"Abstract Multiple sclerosis is a chronic inflammatory demyelinating disease of the central nervous system characterized by multiple disseminated lesions. The pathogenesis of this disease is still insufficiently clarified, and the course of the disease is unpredictable. It happens that severe relapses do not respond to drug therapy and in such cases it is recommended therapeutic plasma exchange (TPE). We present a young man with severe motor relapse, weakness of the left leg, pronounced tremor of the right arm, unstable gait and bilaterally reduction of vision. Brain magnetic resonance imaging showed a large number of chronic demyelinating lesions of the white mass, numerous were merged, with signs of brain reduction. The patient underwent five cycles of TPE, which were conducted every other day. Although TPE led to a disturbance of the hemostasis status and some biochemical parameters, the patient’s response to the applied therapy was good. The improvement of the patient’s condition was reflected through the return of vision and a moderate reduction in neurological deficit. Despite the fact that therapeutic plasma exchange can cause some complications, it should be considered when drug therapy does not lead to improvement in these patients.","PeriodicalId":21582,"journal":{"name":"Serbian Journal of Experimental and Clinical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48924140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overview of Host Defense Peptides with Promising Anti-Breast Cancer Activity 具有抗乳腺癌活性的宿主防御肽综述

Serbian Journal of Experimental and Clinical Research Pub Date : 2022-03-26 DOI: 10.2478/sjecr-2021-0052

Sanja Zornic, Bojana Luković, Andra Jevtović, J. Dimitrijevic, B. S. Markovic, Jelena M. Pantić, G. Radosavljević, N. Arsenijević

{"title":"Overview of Host Defense Peptides with Promising Anti-Breast Cancer Activity","authors":"Sanja Zornic, Bojana Luković, Andra Jevtović, J. Dimitrijevic, B. S. Markovic, Jelena M. Pantić, G. Radosavljević, N. Arsenijević","doi":"10.2478/sjecr-2021-0052","DOIUrl":"https://doi.org/10.2478/sjecr-2021-0052","url":null,"abstract":"Abstract Breast cancer is the leading cause of death among women worldwide. The main limitations of conventional anti-cancer therapy, including breast cancer treatment, are side effects and the development of resistance to chemotherapeutics. Host defense peptides (HDPs) are bioactive compounds of innate immunity isolated from almost all living organisms, which exhibit wide range of biological activities. This review focuses on the anti-cancer effects of HDPs and their therapeutic potential against breast cancer. Numerous HDPs from different sources, including mammalian and amphibian origin, and their chemically modified analogues, exert the spectrum of anti-cancer activities. These effects include direct disruption of cancer cell membrane, induction of apoptosis, inhibition of angiogenesis and cancer cell proliferation, but also the modulation of anti-cancer immune response. Selected examples of HDPs of different origin and their anti-breast cancer capacities have been reviewed. Conclusively, due to their anti-cancer effects accompanied by substantial selectivity for cancer cells and low toxicity for normal cells, HDPs have been widely recognized as possible therapeutic agents.","PeriodicalId":21582,"journal":{"name":"Serbian Journal of Experimental and Clinical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47362648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of Hospitalized COVID-19 Patients in Sumadija District in 2020 2020年Sumadija区住院新冠肺炎患者特征

Serbian Journal of Experimental and Clinical Research Pub Date : 2022-03-26 DOI: 10.2478/sjecr-2021-0059

N. Mihailovic, Ivana Djordjević, Nikola Uskoković, Ana Miljanović, Ljiljana Zdravkovic, Slavica Bukumira, B. Bajić

{"title":"Characteristics of Hospitalized COVID-19 Patients in Sumadija District in 2020","authors":"N. Mihailovic, Ivana Djordjević, Nikola Uskoković, Ana Miljanović, Ljiljana Zdravkovic, Slavica Bukumira, B. Bajić","doi":"10.2478/sjecr-2021-0059","DOIUrl":"https://doi.org/10.2478/sjecr-2021-0059","url":null,"abstract":"Abstract Introduction:The number of hospitalized COVID patients varies in accordance with the waves of the pandemic. The aim of the study was to examine the characteristics of hospitalized COVID-19 patients and predictors of in-hospital died with special reference to the importance of comorbidity. Method: A retrospective cohort study that included all COVID patients hospitalized at the Clinical Center Kragujevac in 2020. The data contained in the Hospitalization Report are described, and the predictors of hospital mortality are defined by binary logistic regression. Results: 1336 COVID patients were hospitalized. The average age of the hospitalized patients was 58.1 ± 16.5 years, 2/3 of them were males. The largest number of hospitalized patients live in Kragujevac - 62.8%. During hospitalization, 19.4% (n = 206) of patients died, who were on average 13 years older (t = 14.13, df = 504.3, p <0.01), and stayed in the hospital 2 days shorter (Z = -5.36, p <0.01) when compared to discharged patients. 86.5% (n = 1155) of hospitalized patients had comorbidities, most often hypertension and other heart diseases, diabetes mellitus and renal failure. Statistically significant predictors of the lethal outcome of hospitalization were patients’ age (OR = 0.94 95% CI = 0.93-0.95), residence (OR = 0.84, 95% CI = 0.75-0.95), length of hospitalization (OR = 1.06, 95% CI = 1.04-1.09) and the presence of comorbidity as the strongest predictor (OR = 5.31, 95% CI = 2.37-11.89). Conclusion: COVID Patients with comorbidities require special attention because comorbidities affect the outcome of hospitalization.","PeriodicalId":21582,"journal":{"name":"Serbian Journal of Experimental and Clinical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41658468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0