{"title":"Plasma lipids of golden Syrian hamsters fed dietary rose hip, sunflower, olive and coconut oils.","authors":"I González, M Escobar, P Olivera","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Plasma lipids of male golden Syrian hamsters fed diets supplemented with 15% (w/w) rose hip, sunflower, olive, or coconut oils during four weeks were assessed. The results confirm the saturated fat hyperlipidemic effect on golden Syrian hamsters fed with the olive oil and coconut oil, reaching the highest triglyceride levels. The monounsaturated (olive oil) or polyunsaturated (rose hip and sunflower oils) fatty acid-rich-vegetable oils have a similar action on the HDL-cholesterol. No statistically significant difference was observed for total cholesterol, HDL-cholesterol and triglyceride plasma levels in the rose hip and sunflower groups, showing that the polyunsaturation degree of both oils does not affect those results. Compared with the plasma levels obtained in the olive and coconut oil groups, rose hip and sunflower oils present a marked hypolipidemic effect, which could be due to specific action of the series n-6 linoleic acid.</p>","PeriodicalId":21473,"journal":{"name":"Revista espanola de fisiologia","volume":"53 2","pages":"199-204"},"PeriodicalIF":0.0,"publicationDate":"1997-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20232683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of gonadoreline and naloxone on induction of ovulation and plasma LH in rabbit.","authors":"P G Rebollar, J M Alvariño, J C Illera, G Silván","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>To study the effect of an opiate antagonist receptor (naloxone) on LH secretion, two groups of seven does were randomly allocated to GnRH (gonadoreline, 20 microgram/animal), and GnRH + naloxone (1.8 mg/kg b.w.) treatment, respectively and another group of eight animals was treated with naloxone alone. Blood samples were obtained immediately before treatment (0 minutes) and at 60, 120 and 180 minutes after injection. Plasma levels of LH were determined by a direct, simple and precise sandwich enzyme immunoassay (EIA), adapted for measuring plasma LH in rabbit. Luteinizing hormone reached maximum values 60 minutes after administration of GnRH or GnRH + naloxone (p < 0.05) and all the females of these groups ovulated. Only 25% of naloxone treated rabbit does ovulated and these animals presented a high LH plasma level at 60 minutes post-treatment (p < 0.05). In non ovulated animals no variations in plasma LH concentrations were observed at 60 minutes, in relation to blood samples obtained at 0, 120 and 180 minutes. Results show a limited effect of this naloxone dose on the anterior pituitary of non lactating adult rabbi does in relation to induced ovulation. No synergic effect was detected when naloxone was administered in addition to gonadoreline.</p>","PeriodicalId":21473,"journal":{"name":"Revista espanola de fisiologia","volume":"53 2","pages":"205-10"},"PeriodicalIF":0.0,"publicationDate":"1997-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20232684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M C Tarrés, S M Martínez, S M Montenegro, N S Figueroa, A E D'Ottavio, J C Picena
{"title":"Influence of gonadectomy in eSS diabetic rats.","authors":"M C Tarrés, S M Martínez, S M Montenegro, N S Figueroa, A E D'Ottavio, J C Picena","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The influence of gonadectomy on some variables related to the diabetic syndrome was studied in the eSS line of rats, a nonobese model of spontaneous non-insulin-dependent diabetes, whose biochemical and histopathological manifestations are more severe in males than in females. Rats were gonadectomized at 90 days of age. Spayed animals showed higher body weight, impaired intolerance to glucose at 9 and 12 months of age, lower insulinemia and a decreased number of large pancreatic islets. Castrated rats revealed lower body weight when compared with controls. However, those males did not evidence impairment in the intolerance to glucose, changes insulinemia or remarkable modifications in endocrine pancreas histology. In kidneys, a lower cellular area in superficial proximal convoluted tubules was noticed. Despite the lower biomass registered in orchidectomized animals, their diabetic evolution was not modified. Conversely, ovariectomy appeared to be a worsening factor.</p>","PeriodicalId":21473,"journal":{"name":"Revista espanola de fisiologia","volume":"53 2","pages":"211-6"},"PeriodicalIF":0.0,"publicationDate":"1997-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20232685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Trout plasma metabolites, blood factors and spleen contraction: responses to exercise.","authors":"P Mendiola, M D Hernández, J de Costa, S Zamora","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Fish react to handling and capture with a burst of exercise that affects them deeply. The present study examines the effect of such severe exercise and the time course of recovery on the hematology (including spleen response) and metabolism of a population of cultured rainbow trout. Exercise was induced by continuous chasing for 5 min when the trout showed signs of exhaustion. Such exercise led to spleen contraction and an increase in haematocrit values. Carbohydrates were mobilized and anaerobic glycolysis produced lactate without significant effect on lipid metabolism. The conclusion is reached that the respiratory properties of rainbow trout blood do not change following severe exercise, while muscle anaerobic metabolism is slightly activated as deduced from the fast and short lactacidemia observed, which may have been related to a reduced stressing component, as the exercise was performed in the same environment in which the fish were reared.</p>","PeriodicalId":21473,"journal":{"name":"Revista espanola de fisiologia","volume":"53 2","pages":"217-23"},"PeriodicalIF":0.0,"publicationDate":"1997-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20232686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"International joint meeting of physiology. XXVIII National Congress of the Spanish Society of Physiological Sciences and the American Physiological Society. Malaga, Costa del Sol, Spain, 4-7 February 1997. Abstracts.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":21473,"journal":{"name":"Revista espanola de fisiologia","volume":"53 1","pages":"5-198"},"PeriodicalIF":0.0,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20127433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J Fuentes, J L Soengas, M Buceta, J Otero, P Rey, E Rebolledo
{"title":"Kidney ATPase response in seawater-transferred rainbow trout (Oncorhynchus mykiss). Effect of salinity and fish size.","authors":"J Fuentes, J L Soengas, M Buceta, J Otero, P Rey, E Rebolledo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Two sizes of domesticated rainbow trout (Oncorhynchus mykiss, 40 +/- 0.67 and 180 +/- 3.9 g) were directly transferred to brackish water (9 ppt) and seawater (28 ppt). Kidney Na(+)-K(+)-ATPase and Mg(2+)-ATPase activities were measured in fresh water, and after long-term seawater adaptation (up to 21 days). Renal Na(+)-K(+)-ATPase activity increased after saltwater loading in small trout, while large trout displayed an unmodified ATPase activity. The smallest trout showed a low but progressive increase in renal Mg(2+)-ATPase activity after the transfer to both salinities. However, ATPase activity remained unchanged or significantly decreased in large trout after the transfer to seawater or brackish water, respectively.</p>","PeriodicalId":21473,"journal":{"name":"Revista espanola de fisiologia","volume":"52 4","pages":"231-8"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20092978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative study of the effect of teicoplanin and vancomycin upon the phagocytic process of peritoneal macrophages.","authors":"C Barriga, I Pedrera, A B Rodríguez","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of Teicoplanin and Vancomycin upon the phagocytic process was compared by evaluating the different activities of the peritoneal macrophage phagocytic function from mice treated with these antibiotics. The results indicated that teicoplanin and vancomycin increased both the substrate adherence and chemotaxis of peritoneal macrophages and that neither antibiotic, at the concentrations of 10, 25, 50, 75 and 100 mg/l, had any chemoattractant capacity for peritoneal macrophages. There was an increase in the attachment of Candida albicans only in the macrophages from mice treated with vancomycin. The phagocytosis of C. albicans, Staphylococcus aureus, Escherichia coli and inert particles as well, as the nitroblue tetrazolium reduction capacity (microbicide capacity) increased in the presence of both antibiotics. The C. albicans digestion capacity increased only in the peritoneal macrophages from mice treated with teicoplanin.</p>","PeriodicalId":21473,"journal":{"name":"Revista espanola de fisiologia","volume":"52 4","pages":"215-22"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20092426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Follicular and luteal progesterone synergize to maintain 5-day cyclicity in rats.","authors":"J E Sánchez-Criado, A Ruiz, M Tébar, J A Mattheij","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The length of the ovarian cycle in rat is determined by the duration of progesterone secretion from the corpora lutea (CL) during diestrus. The action of progesterone secretion from the preovulatory follicles on proestrus is also responsible for the cycle length in 4-day cyclic rats. To study whether follicular and luteal progesterone participate in the maintenance of 5-day cyclicity, the effects of the antiprogestagen RU486 (5 mg on proestrus or estrus) on estrous cycle length and on the serum concentrations of LH in 5-day cyclic rats and in 4-day cycle experimentally induced by the dopamine agonist CB154 (1 mg on estrus) were investigated. Furthermore, serum concentrations of progesterone on the day of ensuing ovulation were measured to see whether activation of the CL function after treatment with RU486 had occurred. Both 5-day and CB154-injected rats had a 3-day estrous cycle after RU486 on proestrus, while RU486 on estrus shortened by 1-day the estrous cycle length in 5-day but not in CB154-injected rats. Basal serum concentrations of LH increased and the LH surge decreased after RU486 treatment in both cycle types. Serum concentrations of progesterone rose only in 5-day rats injected with RU486. These results indicate that the actions of both follicular and luteal progesterone synergize in maintaining the length of the estrous cycle in 5-day cyclic rats and that functionally active CL increase progesterone production only under the action of a complete surge of prolactin.</p>","PeriodicalId":21473,"journal":{"name":"Revista espanola de fisiologia","volume":"52 4","pages":"223-9"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20092977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Regulation of energy balance and adiposity: a model with new approaches.","authors":"J A Martínez, G Frühbeck","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Obesity etiology and treatment have been fraught with disappointment for researchers, because the mechanisms regulating fuel homeostasis and adiposity are incompletely understood. It can now be hypothesized in the light of new evidences that the control of body weight and composition depends upon an axis with three interrelated and self-controlled components: 1) food intake; 2) nutrient turnover and thermogenesis and 3) body fat stores, all of which underly complex feedback mechanisms. This approach considers two of the most relevant recent findings in the field (leptin and beta 3-adrenoceptors), adding new views to previous metabolic models of obesity. This perspective supplies some additional clues to the understanding of body composition regulation as well as the potential involvement of genetic and hypothalamic disorders in the onset of obesity.</p>","PeriodicalId":21473,"journal":{"name":"Revista espanola de fisiologia","volume":"52 4","pages":"255-8"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20092981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G Frühbeck, B Muguerza, I Castilla-Cortázar, S Santidrián
{"title":"Effect of aging on the rate of muscle protein turnover in rat.","authors":"G Frühbeck, B Muguerza, I Castilla-Cortázar, S Santidrián","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The urinary excretion of Nt-methylhistidine (e-methylhistidine, 3-MH, an index related to myofibrillar protein breakdown), as well as the rate of L-[14C]-tyrosine incorporation into gastrocnemius muscle (ks, fractional rate of muscle protein synthesis, evaluated by the constant infusion method) have been measured to assess the effect of aging on the rate of skeletal muscle protein turnover. In addition, nucleic acids, muscle protein and serum corticosterone levels were determined. Weaning rats were fed a 10% lactalbumin diet and killed in groups of seven when they were 35, 60, 120 and 300 days old. Apart from the rate of growth, no major differences were found between 35- and 60-day old animals. However, as compared to the youngest rats, 120-day old rats showed a significant reduction in the relative weight of the four muscles excised. Plasma corticosterone levels, increased as the animals became older. Finally, in the 300-day old rats, the reduced rate of growth was accompanied by a significant reduction in the relative organ weight (with the exception of soleus), 3-MH and Ks. It is concluded that aging caused a reduction in the rates of both protein breakdown and synthesis. The reduced muscle breakdown may not be due to a relative reduced muscle mass in elder rats since urinary 3-MH remained low even when expressed per creatinine output.</p>","PeriodicalId":21473,"journal":{"name":"Revista espanola de fisiologia","volume":"52 4","pages":"207-14"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20092425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}